FastQCFastQC Report
Thu 26 May 2016
SRR936740_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936740_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13214
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.5902830331466626No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.4994702588164069No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.46163160284546695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.4086574844861511No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT410.31027697896170725No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT350.2648705917965794No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.2270319358256395No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT230.1740578174663236RNA PCR Primer, Index 33 (95% over 22bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA220.1664900862721356No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.15892235507794764No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.15135462388375964No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA180.13621916149538368No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT180.13621916149538368No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.12108369910700773No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA160.12108369910700773No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT140.10594823671863177RNA PCR Primer, Index 33 (95% over 24bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG300.003851481531.71736154-55
GCCGTCT404.4337497E-429.62239646-47
CGTCTTC350.008181822527.1863148-49
ACATGGG700.008186961527.0833343
GTACATG700.008186961527.0833341
ATGCCGT350.00833528827.08333444-45
AGTACAT559.2749135E-525.85227212-13
GAGTACT801.7814145E-623.6979186
GTACTTT853.0174506E-622.303928
TACATGG850.001751490622.303922
AGTACTT900.00256624421.0648147
GGTATCA1404.5789583E-420.31251
TACTTTT950.003679531619.956149
GTATCAA1901.7245095E-417.4616221
ACTTTTT1102.7989663E-517.23484816-17
TATCAAC2053.082183E-416.1839452
AGAGTAC1854.474714E-815.37162110-11
ATCAACG2205.2771927E-415.0804933
TCAACGC2256.2596047E-414.745374
AACGCAG2307.3956285E-414.4248196
Produced by FastQC (version 0.11.5)