##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936740_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13214 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.97010746178296 31.0 30.0 33.0 28.0 34.0 2 31.11147268049039 31.0 31.0 34.0 28.0 34.0 3 31.18147419403663 31.0 31.0 34.0 27.0 34.0 4 34.963826244891784 35.0 35.0 37.0 32.0 37.0 5 34.654457393673376 35.0 35.0 37.0 30.0 37.0 6 34.69607991524141 35.0 35.0 37.0 30.0 37.0 7 34.56076888148933 35.0 35.0 37.0 30.0 37.0 8 34.553730891478736 35.0 35.0 37.0 30.0 37.0 9 35.92417133343424 38.0 35.0 39.0 30.0 39.0 10-11 35.80494172846981 37.0 35.0 39.0 30.0 39.0 12-13 35.642159830482825 37.0 35.0 39.0 30.0 39.0 14-15 36.4802103829272 38.0 35.0 40.0 30.0 41.0 16-17 36.38644619343121 38.0 35.0 40.0 30.0 41.0 18-19 36.575677311941874 38.0 35.0 40.0 30.0 41.0 20-21 36.57276373543212 38.0 35.0 40.0 30.0 41.0 22-23 36.39991675495686 38.0 34.5 40.0 29.5 41.0 24-25 36.19210685636446 38.0 34.0 40.0 29.5 41.0 26-27 35.786514303011955 38.0 34.0 40.0 27.0 41.0 28-29 35.539276524897836 38.0 34.0 40.0 27.0 41.0 30-31 35.35504010897533 38.0 34.0 40.0 27.0 41.0 32-33 35.06292568487967 38.0 33.0 40.0 25.5 41.0 34-35 34.728394127440595 38.0 33.0 40.0 25.0 41.0 36-37 34.374602694112305 37.0 33.0 40.0 24.0 41.0 38-39 34.15805206599062 37.0 33.0 40.0 24.0 41.0 40-41 33.80501740578174 37.0 33.0 40.0 23.0 41.0 42-43 33.45796125321628 37.0 32.0 40.0 21.5 41.0 44-45 33.13050552444377 37.0 31.0 40.0 19.5 41.0 46-47 32.569169063114884 37.0 31.0 40.0 16.0 41.0 48-49 32.0209626154079 36.0 30.0 40.0 11.0 41.0 50-51 31.607386105645528 36.0 30.0 39.5 8.0 41.0 52-53 31.22544271227486 35.5 29.0 39.0 7.0 41.0 54-55 30.918911760254275 35.0 29.0 39.0 2.0 40.5 56-57 30.525011351596792 35.0 28.0 39.0 2.0 40.0 58-59 30.011313758135312 35.0 28.0 38.5 2.0 40.0 60-61 29.354888754351443 34.0 26.5 38.0 2.0 40.0 62-63 28.85352656273649 34.0 26.0 38.0 2.0 40.0 64-65 28.361775389738156 34.0 25.5 37.5 2.0 40.0 66-67 27.83456939609505 33.5 24.5 37.0 2.0 39.5 68-69 27.36760254275768 33.0 23.0 36.0 2.0 39.0 70-71 27.03095202058423 33.0 22.5 36.0 2.0 39.0 72-73 26.523649159981836 32.5 21.5 35.5 2.0 38.5 74-75 26.02989253821704 32.0 20.5 35.0 2.0 37.5 76-77 24.61559709399122 30.5 19.5 34.0 2.0 36.0 78-79 25.302406538519755 31.5 19.5 35.0 2.0 36.0 80-81 25.085666717118208 32.0 18.0 35.0 2.0 36.0 82-83 24.744589072196156 31.5 16.0 35.0 2.0 36.0 84-85 24.326169214469502 31.0 9.5 34.0 2.0 35.0 86-87 23.81765551687604 31.0 4.5 34.0 2.0 35.0 88-89 23.227977902224914 30.5 2.0 34.0 2.0 35.0 90-91 22.615748448615108 30.0 2.0 34.0 2.0 35.0 92-93 21.95667473891327 29.5 2.0 34.0 2.0 35.0 94-95 21.26365975480551 29.0 2.0 34.0 2.0 35.0 96-97 20.41066293325261 29.0 2.0 34.0 2.0 35.0 98-99 19.548774027546543 27.5 2.0 34.0 2.0 35.0 100-101 16.94971242621462 21.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.20276549600997384 1101 2 0.08396325981344077 1101 3 -0.1786727858878585 1101 4 0.48336943302477664 1101 5 0.5159252087101436 1101 6 0.7257271621012364 1101 7 0.8037894626819195 1101 8 0.4044371282652577 1101 9 -1.5970983273602712 1101 10-11 -0.5907600569365172 1101 12-13 -1.8377980063067412 1101 14-15 -0.32513361927424 1101 16-17 -0.4102436978700794 1101 18-19 -1.3108485507037813 1101 20-21 -0.48211370218494665 1101 22-23 0.8833946694815324 1101 24-25 0.8160989231186875 1101 26-27 0.7875145118022004 1101 28-29 0.41262445300818484 1101 30-31 0.22752741582016256 1101 32-33 0.5796148351410437 1101 34-35 -0.338067589239472 1101 36-37 -0.6266701733383684 1101 38-39 -0.6393058305052932 1101 40-41 1.4705921172373309 1101 42-43 -0.09044999416094157 1101 44-45 -0.9620309314358053 1101 46-47 1.3570717855299606 1101 48-49 -0.5897539751744922 1101 50-51 -0.7083003789001019 1101 52-53 -0.7772816061763663 1101 54-55 -1.0530667351439398 1101 56-57 -1.5222765728280478 1101 58-59 -0.09432921211414325 1101 60-61 -0.4965961870373583 1101 62-63 -0.36774348931978196 1101 64-65 0.5824556821684723 1101 66-67 2.724850333490039 1101 68-69 -0.9546994965308464 1101 70-71 1.147478991596639 1101 72-73 0.2648971994100613 1101 74-75 0.7245583924007555 1101 76-77 2.4882161811160017 1101 78-79 1.5103671094020505 1101 80-81 -1.2298449002148395 1101 82-83 -0.0677784931231713 1101 84-85 0.37286923358843893 1101 86-87 -1.2864196635336427 1101 88-89 -0.5161509199882346 1101 90-91 0.8901604612197396 1101 92-93 2.2916961723257607 1101 94-95 1.301805298289679 1101 96-97 1.4964638528172607 1101 98-99 -0.09047960694330115 1101 100-101 3.271422113000707 1102 1 -0.3758424190868972 1102 2 1.1096042854544699 1102 3 0.9110708038557291 1102 4 1.1115745612299008 1102 5 1.297976490761421 1102 6 1.270598956973032 1102 7 0.7589176678101239 1102 8 -0.5122295384014066 1102 9 1.6785426982807579 1102 10-11 0.38039378921732947 1102 12-13 1.2679712244624852 1102 14-15 1.4889689448283292 1102 16-17 2.221166558540169 1102 18-19 2.4583822185269852 1102 20-21 2.5210914260201918 1102 22-23 1.3353177464046126 1102 24-25 0.9378937949135633 1102 26-27 2.2009760502637405 1102 28-29 1.4062141965979364 1102 30-31 2.044835108127856 1102 32-33 2.9001276556538613 1102 34-35 2.088214462042586 1102 36-37 4.13615033948215 1102 38-39 3.1074890412895755 1102 40-41 3.345592117237331 1102 42-43 2.377498723787774 1102 44-45 3.1213024018975233 1102 46-47 2.2224564009145737 1102 48-49 2.8910152555947377 1102 50-51 2.7404175698178435 1102 52-53 2.6361799322851738 1102 54-55 1.8668050597278487 1102 56-57 3.0706721451206676 1102 58-59 3.5178502750653458 1102 60-61 2.426480736039565 1102 62-63 1.238025741449448 1102 64-65 3.5183531180659138 1102 66-67 3.833824692464397 1102 68-69 2.144659477828128 1102 70-71 1.102607196724847 1102 72-73 0.7584869429998022 1102 74-75 2.170071212913573 1102 76-77 1.965780283680104 1102 78-79 0.26357223760717474 1102 80-81 2.4656679202979817 1102 82-83 3.355298429953752 1102 84-85 3.3600487207679244 1102 86-87 3.934734182620204 1102 88-89 4.214618310780995 1102 90-91 2.6401604612197396 1102 92-93 1.9423371979667898 1102 94-95 1.9364206829050659 1102 96-97 3.788130519483925 1102 98-99 4.5793921879284945 1102 100-101 3.7329605745391667 1103 1 0.8741575809131028 1103 2 1.6096042854544699 1103 3 1.2444041371890648 1103 4 1.4449078945632365 1103 5 -0.4520235092385789 1103 6 1.270598956973032 1103 7 0.8422510011434596 1103 8 1.1544371282652577 1103 9 1.0952093649474222 1103 10-11 1.1720604558839938 1103 12-13 1.1013045577958138 1103 14-15 1.447302278161665 1103 16-17 0.6794998918735118 1103 18-19 -0.37495111480634336 1103 20-21 0.812758092686849 1103 22-23 0.876984413071284 1103 24-25 1.3128937949135562 1103 26-27 0.9926427169304048 1103 28-29 1.989547529931265 1103 30-31 2.3781684414611917 1103 32-33 2.233460988987197 1103 34-35 0.9632144620425862 1103 36-37 2.2194836728154783 1103 38-39 1.3574890412895826 1103 40-41 1.2622587839040023 1103 42-43 2.877498723787774 1103 44-45 2.829635735230859 1103 46-47 3.1807897342479023 1103 48-49 3.432681922261409 1103 50-51 3.157084236484515 1103 52-53 4.344513265618502 1103 54-55 5.03347172639452 1103 56-57 4.15400547845401 1103 58-59 2.6428502750653386 1103 60-61 4.384814069372894 1103 62-63 4.988025741449448 1103 64-65 5.685019784732571 1103 66-67 6.500491359131061 1103 68-69 6.186326144494789 1103 70-71 6.435940530058179 1103 72-73 4.133486942999802 1103 74-75 4.461737879580241 1103 76-77 5.632446950346772 1103 78-79 7.22190557094051 1103 80-81 7.757334586964642 1103 82-83 7.0219650966204235 1103 84-85 5.9433820541012565 1103 86-87 6.018067515953536 1103 88-89 7.089618310780995 1103 90-91 6.848493794553075 1103 92-93 8.984003864633454 1103 94-95 10.311420682905066 1103 96-97 8.37146385281726 1103 98-99 9.579392187928491 1103 100-101 8.732960574539167 1104 1 0.14688485364037618 1104 2 -0.31463813878795577 1104 3 -0.16468677190184522 1104 4 -0.4187284690731303 1104 5 0.040400733185663285 1104 6 -0.4490980127239368 1104 7 0.614978273870733 1104 8 0.5862553100834376 1104 9 -0.3593360895980311 1104 10-11 -0.15369711987357704 1104 12-13 -1.5161196846284213 1104 14-15 -0.6852734794141 1104 16-17 -0.3962576838840661 1104 18-19 -0.3597995996548349 1104 20-21 -1.8842116042828394 1104 22-23 0.5057722918591594 1104 24-25 -0.14543953841977242 1104 26-27 0.1896124139001003 1104 28-29 1.671365711749452 1104 30-31 0.6471078354005826 1104 32-33 0.733460988987197 1104 34-35 0.5010932499213681 1104 36-37 -0.7805163271845217 1104 38-39 2.7627920715926066 1104 40-41 2.6978648445100646 1104 42-43 3.2032562995453517 1104 44-45 2.2652417958369213 1104 46-47 3.3815473100054803 1104 48-49 3.413742528322004 1104 50-51 3.361629691029968 1104 52-53 4.715725386830627 1104 54-55 3.8175626354854266 1104 56-57 3.998702448150979 1104 58-59 3.6504260326411035 1104 60-61 0.5628443724032017 1104 62-63 0.5448439232676279 1104 64-65 2.397140996853789 1104 66-67 5.189885298525006 1104 68-69 4.034810992979647 1104 70-71 3.8677587118763626 1104 72-73 2.0236384581513214 1104 74-75 2.832950000792362 1104 76-77 2.575628768528592 1104 78-79 -1.3462762472413097 1104 80-81 -1.0305442009141395 1104 82-83 -3.599247024591701 1104 84-85 -3.6551027943835876 1104 86-87 -1.4857203628343427 1104 88-89 -0.28538168921900464 1104 90-91 -1.700748629689354 1104 92-93 -0.7887234080938192 1104 94-95 -2.222670226185844 1104 96-97 1.0419183982718074 1104 98-99 0.7801497636860688 1104 100-101 2.7784151199937135 1105 1 0.32870303545855606 1105 2 -0.31463813878795577 1105 3 1.562585955370885 1105 4 0.3085442581996034 1105 5 0.7676734604583899 1105 6 0.6418110781851496 1105 7 1.614978273870733 1105 8 -0.23192650809837545 1105 9 0.36793663767469553 1105 10-11 0.9372119710355165 1105 12-13 1.6656984971897586 1105 14-15 1.1329083387677201 1105 16-17 1.6946514070250274 1105 18-19 1.9583822185269852 1105 20-21 2.2521520320807937 1105 22-23 0.09668138276825289 1105 24-25 1.0363786433984146 1105 26-27 1.3714305957182873 1105 28-29 2.989547529931265 1105 30-31 3.3289260172187625 1105 32-33 1.6880064435326503 1105 34-35 2.182911431739555 1105 36-37 2.4922109455427446 1105 38-39 2.535519344319887 1105 40-41 2.1978648445100646 1105 42-43 0.7941653904544452 1105 44-45 3.492514523109648 1105 46-47 3.3360927645509335 1105 48-49 3.550106164685644 1105 50-51 3.4525387819390616 1105 52-53 2.715725386830627 1105 54-55 2.0448353627581604 1105 56-57 0.5896115390600656 1105 58-59 1.4686078508229201 1105 60-61 -1.0280647185058882 1105 62-63 -2.4097015312778254 1105 64-65 -1.330131730418941 1105 66-67 -1.5373874287477207 1105 68-69 -0.19246173429308655 1105 70-71 -0.08678674266909425 1105 72-73 1.0236384581513178 1105 74-75 -0.3034136355712711 1105 76-77 1.075628768528592 1105 78-79 1.6537237527586903 1105 80-81 2.060364889994947 1105 82-83 0.5825711572264787 1105 84-85 0.5267153874345922 1105 86-87 0.2415523644383839 1105 88-89 2.1237092198719054 1105 90-91 0.07197864303791945 1105 92-93 -1.7432688626392725 1105 94-95 -0.6317611352767543 1105 96-97 0.08737294372635063 1105 98-99 1.0983315818678854 1105 100-101 2.50568784726644 1106 1 -0.3401281333726125 1106 2 0.4191280949782765 1106 3 0.6729755657604919 1106 4 0.8734793231346671 1106 5 0.4170241098090415 1106 6 -0.5984486620745884 1106 7 0.913679572572029 1106 8 1.2615799854081118 1106 9 1.3094950792331375 1106 10-11 0.21967950350304477 1106 12-13 0.10725693874820053 1106 14-15 1.3699213257807088 1106 16-17 0.941404653778271 1106 18-19 1.494096504241277 1106 20-21 1.1222819022106592 1106 22-23 0.5674606035474739 1106 24-25 0.6045604615802276 1106 26-27 1.3616903359780252 1106 28-29 -0.760452470068735 1106 30-31 1.9555493938421478 1106 32-33 2.233460988987197 1106 34-35 1.903690652518776 1106 36-37 1.2909122442440477 1106 38-39 0.3277271365276775 1106 40-41 2.6491635458087615 1106 42-43 1.6155939618830146 1106 44-45 3.1808262114213335 1106 46-47 1.9724564009145737 1106 48-49 1.605300969880446 1106 50-51 2.657084236484515 1106 52-53 4.0468942179994585 1106 54-55 4.444186012108812 1106 56-57 3.3980530975016237 1106 58-59 4.488088370303441 1106 60-61 3.2479093074681344 1106 62-63 3.2915971700208786 1106 64-65 2.137400737113527 1106 66-67 3.131443740083448 1106 68-69 2.2458499540186025 1106 70-71 2.3645119586296097 1106 72-73 3.1989631334759956 1106 74-75 3.4855474033897664 1106 76-77 3.007446950346772 1106 78-79 3.4719055709405104 1106 80-81 3.4597155393455985 1106 82-83 3.4624412870966097 1106 84-85 3.63385824457745 1106 86-87 3.5954484683344887 1106 88-89 2.3574754536381377 1106 90-91 3.31873188979117 1106 92-93 3.4125752932048847 1106 94-95 4.007849254333639 1106 96-97 5.175035281388691 1106 98-99 3.2639159974523047 1106 100-101 5.554389145967736 1107 1 0.040824247579770656 1107 2 -0.7237290478788658 1107 3 0.7444041371890648 1107 4 -0.22175877210342776 1107 5 0.13130982409475678 1107 6 -0.8127343763603037 1107 7 1.008917667810124 1107 8 0.9044371282652577 1107 9 1.0118760316140865 1107 10-11 0.17206045588400087 1107 12-13 -1.898695442204179 1107 14-15 -0.1360310551716779 1107 16-17 -0.4455001081264882 1107 18-19 0.5417155518603138 1107 20-21 -0.27057524064647964 1107 22-23 1.751984413071277 1107 24-25 -0.3954395384197724 1107 26-27 2.3259760502637405 1107 28-29 2.4062141965979293 1107 30-31 1.7115017747945274 1107 32-33 0.4417943223205256 1107 34-35 0.2548811287092434 1107 36-37 1.8444836728154783 1107 38-39 2.8574890412895755 1107 40-41 2.470592117237331 1107 42-43 2.4608320571211166 1107 44-45 1.0379690685641947 1107 46-47 0.6807897342479023 1107 48-49 -0.06731807773859799 1107 50-51 0.32375090315117916 1107 52-53 -3.5304867343814905 1107 54-55 -1.8415282736054799 1107 56-57 -1.0959945215459967 1107 58-59 -0.6488163916013221 1107 60-61 -0.19851926396043496 1107 62-63 -1.011974258550552 1107 64-65 -0.3983135486007576 1107 66-67 -1.6245086408689318 1107 68-69 -2.1053405221718755 1107 70-71 0.5609405300581791 1107 72-73 0.38348694299980224 1107 74-75 -0.5382621204197555 1107 76-77 -0.6592197163198925 1107 78-79 0.09690557094051044 1107 80-81 -0.8676654130353505 1107 82-83 1.4386317632870878 1107 84-85 2.9850487207679244 1107 86-87 -2.8569324840464603 1107 88-89 -1.077048355885669 1107 90-91 0.4318271278864074 1107 92-93 0.6923371979667898 1107 94-95 1.2280873495717302 1107 96-97 0.288130519483925 1107 98-99 1.037725521261823 1107 100-101 0.14962724120583104 1108 1 -0.45917575242022934 1108 2 0.04293761878779989 1108 3 0.34440413718906626 1108 4 0.14490789456323938 1108 5 0.5979764907614253 1108 6 0.7872656236396978 1108 7 0.7422510011434582 1108 8 1.4044371282652577 1108 9 1.5618760316140907 1108 10-11 0.25539378921732947 1108 12-13 0.9263045577958167 1108 14-15 1.1389689448283278 1108 16-17 0.9461665585401775 1108 18-19 1.225048885193651 1108 20-21 1.879424759353526 1108 22-23 0.4269844130712812 1108 24-25 0.42122712824689046 1108 26-27 0.992642716930412 1108 28-29 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2311 82-83 -0.5018444271891056 2311 84-85 -0.8661417554225501 2311 86-87 -1.7974086745226536 2311 88-89 -0.35681026064757404 2311 90-91 0.03301760407688192 2311 92-93 0.44828957891916943 2311 94-95 0.3292778257622082 2311 96-97 -4.217821861468455 2311 98-99 -3.486084002547699 2311 100-101 -2.7670394254608333 2312 1 -0.6258424190868972 2312 2 -0.7951576193074388 2312 3 0.1729755657604919 2312 4 -0.19794924829390936 2312 5 0.05988125266618738 2312 6 -2.1698772335031578 2312 7 -0.5148918559993945 2312 8 0.7615799854081118 2312 9 -0.33336206362400844 2312 10-11 0.5053937892173295 2312 12-13 1.1072569387481934 2312 14-15 -0.4515072456478677 2312 16-17 -0.4871667747931596 2312 18-19 0.24409650424126994 2312 20-21 1.265139045067805 2312 22-23 -0.14682511073824855 2312 24-25 -0.6097252527054877 2312 26-27 -0.35259537830769005 2312 28-29 1.0609761013598344 2312 30-31 -1.0087363204435746 2312 32-33 0.6263181318443358 2312 34-35 -0.1677379189098005 2312 36-37 -0.4590877557559523 2312 38-39 -0.1365585777580378 2312 40-41 -0.7436935970483844 2312 42-43 -1.2058346095455548 2312 44-45 -0.49774521715009 2312 46-47 -0.45611502765685685 2312 48-49 -0.5018418872624082 2312 50-51 0.9785128079130843 2312 52-53 0.11832278942803498 2312 54-55 -0.3058139878911952 2312 56-57 0.4337673832159119 2312 58-59 -2.3333402011251323 2312 60-61 -0.07351926396043496 2312 62-63 -1.7084028299791214 2312 64-65 -2.5768849771721882 2312 66-67 -3.2971276884879828 2312 68-69 -3.611292903124255 2312 70-71 -4.528345184227536 2312 72-73 -3.9438940093811468 2312 74-75 -3.121595453753091 2312 76-77 -4.135410192510374 2312 78-79 -3.992380143345205 2312 80-81 -3.325998746368686 2312 82-83 -2.9661301414748173 2312 84-85 -3.080427469708262 2312 86-87 -0.7616943888083654 2312 88-89 -2.6425245463618623 2312 90-91 -2.788410967351691 2312 92-93 -5.4445675639379765 2312 94-95 -4.6350078885235035 2312 96-97 -4.467821861468455 2312 98-99 -6.164655431119126 2312 100-101 -10.481325139746547 2313 1 -0.8591757524202315 2313 2 -0.9570623812122001 2313 3 -0.3222625294776016 2313 4 -1.588425438770095 2313 5 -3.268690175905242 2313 6 -1.2127343763603022 2313 7 -2.3910823321898746 2313 8 0.004437128265259105 2313 9 -1.5047906350525793 2313 10-11 -0.777939544116002 2313 12-13 -3.140362108870846 2313 14-15 -3.0276977218383436 2313 16-17 -1.020500108126491 2313 18-19 -1.7416177814730176 2313 20-21 -1.253908573979814 2313 22-23 -1.5730155869287188 2313 24-25 -1.9121062050864381 2313 26-27 -3.9740239497362566 2313 28-29 -3.9437858034020685 2313 30-31 -4.1801648918721455 2313 32-33 -3.399872344346136 2313 34-35 -4.220118871290751 2313 36-37 -3.7471829938511902 2313 38-39 -3.5008442920437517 2313 40-41 -6.162741216096002 2313 42-43 -3.139167942878885 2313 44-45 -3.2953642647691375 2313 46-47 -3.860876932418762 2313 48-49 -2.3589847444052623 2313 50-51 -2.4929157635154837 2313 52-53 -3.2721534010481577 2313 54-55 -1.9248616069388156 2313 56-57 -4.137661188212661 2313 58-59 -2.573816391601323 2313 60-61 -0.7735192639604378 2313 62-63 -2.2036409252172184 2313 64-65 -5.8483135486007605 2313 66-67 -4.782841974202267 2313 68-69 -1.847007188838539 2313 70-71 -0.0807261366084866 2313 72-73 -1.158179723666862 2313 74-75 -2.688262120419761 2313 76-77 -1.6758863829865618 2313 78-79 -2.994761095726158 2313 80-81 -3.2759987463686855 2313 82-83 -1.644701570046248 2313 84-85 0.5600487207679237 2313 86-87 0.8597341826202047 2313 88-89 0.41461831078099465 2313 90-91 -1.3431728721135947 2313 92-93 -2.1493294686998787 2313 94-95 -2.5135793170949334 2313 96-97 -2.936869480516073 2313 98-99 -1.0622744787381713 2313 100-101 -2.933706092127501 2314 1 0.707490914246435 2314 2 0.7762709521211342 2314 3 0.9944041371890648 2314 4 0.9449078945632365 2314 5 1.4646431574280925 2314 6 1.520598956973032 2314 7 1.4255843344767953 2314 8 0.821103794931922 2314 9 -0.15479063505257784 2314 10-11 0.7970604558840009 2314 12-13 -0.19036210887085048 2314 14-15 2.113968944828322 2314 16-17 1.0544998918735118 2314 18-19 1.4583822185269852 2314 20-21 1.4794247593535204 2314 22-23 0.876984413071284 2314 24-25 1.3128937949135633 2314 26-27 0.742642716930412 2314 28-29 2.0312141965979364 2314 30-31 0.8781684414611846 2314 32-33 2.9001276556538613 2314 34-35 0.5048811287092434 2314 36-37 -0.2805163271845217 2314 38-39 -1.2258442920437531 2314 40-41 -0.7794078827626691 2314 42-43 -1.1641679428788905 2314 44-45 -1.045364264769134 2314 46-47 -1.319210265752094 2314 48-49 -2.4839847444052623 2314 50-51 -0.7595824301821494 2314 52-53 -0.4471534010481619 2314 54-55 -1.924861606938812 2314 56-57 0.029005478454003253 2314 58-59 0.6011836083986779 2314 60-61 0.9681474027062293 2314 62-63 0.5713590747827837 2314 64-65 -0.3566468819340898 2314 66-67 -0.9578419742022675 2314 68-69 0.1863261444947959 2314 70-71 0.5609405300581791 2314 72-73 0.30015360966647364 2314 74-75 -0.24659545375309122 2314 76-77 0.6741136170134361 2314 78-79 0.13857223760717474 2314 80-81 -0.7843320797020219 2314 82-83 -1.4363682367129158 2314 84-85 0.27671538743459223 2314 86-87 0.3930675159535397 2314 88-89 0.7979516441143311 2314 90-91 -0.6515062054469283 2314 92-93 0.8590038646334541 2314 94-95 1.8947540162384016 2314 96-97 0.8297971861505964 2314 98-99 -2.087274478738177 2314 100-101 -2.3503727587941654 2315 1 -1.392509085753563 2315 2 0.04293761878779989 2315 3 -0.5222625294776009 2315 4 -1.588425438770095 2315 5 -0.20202350923857892 2315 6 0.520598956973032 2315 7 0.27558433447678965 2315 8 0.13777046159859196 2315 9 -0.771457301719245 2315 10-11 -0.8779395441160034 2315 12-13 -3.1070287755375148 2315 14-15 -0.6276977218383379 2315 16-17 -0.18716677479316246 2315 18-19 0.15838221852698808 2315 20-21 -1.1539085739798125 2315 22-23 -1.1063489202620502 2315 24-25 0.18789379491355618 2315 26-27 -0.3073572830695923 2315 28-29 -0.4437858034020721 2315 30-31 -1.2468315585388083 2315 32-33 -0.3665390110128044 2315 34-35 0.17988112870924766 2315 36-37 -2.1805163271845203 2315 38-39 -1.4341776253770817 2315 40-41 -2.7627412160960034 2315 42-43 -2.3391679428788876 2315 44-45 -4.028697598102472 2315 46-47 -5.7608769324187605 2315 48-49 -4.592318077738597 2315 50-51 -2.9595824301821523 2315 52-53 -4.772153401048158 2315 54-55 -4.9915282736054785 2315 56-57 -5.53766118821266 2315 58-59 -2.94048305826799 2315 60-61 -3.3401859306271007 2315 62-63 -5.503640925217219 2315 64-65 -5.548313548600756 2315 66-67 -3.982841974202266 2315 68-69 -4.180340522171871 2315 70-71 -2.2473928032751544 2315 72-73 -4.891513057000196 2315 74-75 -7.02159545375309 2315 76-77 -6.509219716319894 2315 78-79 -5.794761095726155 2315 80-81 -5.209332079702019 2315 82-83 -5.878034903379582 2315 84-85 -5.106617945898741 2315 86-87 -5.3735991507131295 2315 88-89 -5.352048355885671 2315 90-91 -4.60983953878026 2315 92-93 -5.082662802033212 2315 94-95 -7.813579317094934 2315 96-97 -6.170202813849407 2315 98-99 -6.795607812071509 2315 100-101 -5.8003727587941665 2316 1 -0.39856969181417057 2316 2 -1.3146381387879558 2316 3 -1.0737776809927517 2316 4 -2.327819378164037 2316 5 -1.050508357723423 2316 6 -0.3581889218148504 2316 7 -0.6577489988565404 2316 8 -0.41374468991656244 2316 9 -0.6320633623253045 2316 10-11 -1.2900607562372173 2316 12-13 -1.2888469573556947 2316 14-15 -3.821637115777733 2316 16-17 -2.7144395020658862 2316 18-19 -1.7234359632911946 2316 20-21 -1.1114843315555731 2316 22-23 -0.13059134450448084 2316 24-25 -0.09998499296522567 2316 26-27 -1.8558421315544393 2316 28-29 -0.010452470068734954 2316 30-31 -0.6710739827812375 2316 32-33 -1.9938117382855296 2316 34-35 -2.180724931896812 2316 36-37 -1.5986981453663347 2316 38-39 1.0809738897744339 2316 40-41 -0.029407882762669146 2316 42-43 -0.5694709731819216 2316 44-45 -0.9620309314358053 2316 46-47 -0.5275435990854263 2316 48-49 0.595560710140191 2316 50-51 0.7707206001208817 2316 52-53 0.21572538683062703 2316 54-55 2.8175626354854266 2316 56-57 1.362338811787339 2316 58-59 -1.1223012400861734 2316 60-61 1.4719352814941118 2316 62-63 1.2721166505403545 2316 64-65 -2.011949912237121 2316 66-67 2.326248934888639 2316 68-69 -1.1924617342930866 2316 70-71 -2.814059469941821 2316 72-73 -0.2036342691214088 2316 74-75 0.24204090988327565 2316 76-77 -0.37891668601686135 2316 78-79 -3.437185338150396 2316 80-81 -4.712362382732319 2316 82-83 -2.6901561155007947 2316 84-85 -1.1096482489290445 2316 86-87 -0.12208399919797586 2316 88-89 0.12370921987190542 2316 90-91 -1.5643849933257137 2316 92-93 -1.5614506808210926 2316 94-95 -1.6772156807312975 2316 96-97 1.7237365800899873 2316 98-99 -1.2198502363139347 2316 100-101 -1.9943121527335599 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 4.0 6 5.0 7 20.0 8 46.0 9 54.0 10 73.0 11 119.0 12 118.0 13 145.0 14 176.0 15 220.0 16 258.0 17 269.0 18 240.0 19 189.0 20 215.0 21 220.0 22 221.0 23 257.0 24 271.0 25 283.0 26 321.0 27 367.0 28 403.0 29 517.0 30 553.0 31 570.0 32 669.0 33 751.0 34 845.0 35 1026.0 36 1300.0 37 1564.0 38 879.0 39 44.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.08477141992128 22.434150772025433 16.061156524371782 21.4199212836815 2 15.370062055395792 22.99076736794309 38.595429090358714 23.04374148630241 3 18.548509156954747 27.803844407446647 30.861207809898595 22.786438625700015 4 11.9645830180112 19.0177084909944 35.976994097169666 33.040714393824736 5 12.388375964885727 39.45815044649614 33.403965491145755 14.749508097472377 6 28.636294838807324 38.13379748751324 17.156046617224156 16.073861056455275 7 25.518389586801877 35.10670500983805 20.463145149084305 18.911760254275766 8 26.873013470561524 34.01695171787498 20.016649008627212 19.09338580293628 9 26.645981534735885 16.815498713485695 19.638262448917814 36.9002573028606 10-11 24.133494778265476 27.70168003632511 26.53624943242016 21.628575752989253 12-13 26.123808082336915 24.83729377932496 27.54275768124716 21.496140457090966 14-15 22.775087028908732 26.316785227788706 26.275162706220673 24.632965037081885 16-17 21.14045709096413 30.24065385197518 26.26381110942939 22.3550779476313 18-19 21.113970031784472 28.0233086120781 29.50280006054185 21.35992129559558 20-21 21.81776903284395 26.42651733010443 29.64658695323142 22.10912668382019 22-23 21.99561071590737 27.30059028303315 29.434690479794156 21.269108521265327 24-25 21.836688360829424 27.614651127591948 29.64658695323142 20.902073558347205 26-27 21.24640532768276 27.682760708339636 30.7287725140003 20.342061449977297 28-29 21.348569698804297 27.016800363251097 30.562282427728167 21.07234751021644 30-31 21.212350537308915 27.04707128802785 30.687149992432268 21.053428182230967 32-33 21.357753727389692 27.813516990842352 30.364035419662454 20.464693862105502 34-35 21.42424701074618 26.92598758892084 30.7930982291509 20.85666717118208 36-37 21.533979113061903 27.209777508702892 29.945512335401848 21.310731042833357 38-39 21.129105494172848 26.354623883759647 31.466626305433632 21.049644316633874 40-41 21.01558952626003 26.755713637051613 31.13364613288936 21.095050703799 42-43 20.96340863510803 26.69996594392099 30.71479925833428 21.621826162636697 44-45 20.98910246708037 27.262751627062208 29.76767065233843 21.980475253518993 46-47 21.62479188739216 27.319509611018617 28.666565763584078 22.389132738005145 48-49 21.54911457545028 26.668684728318446 29.184955350385955 22.597245345845316 50-51 21.250189193279855 27.308158014227335 28.95413954896322 22.487513243529587 52-53 21.745875586499167 27.470864234902376 28.897381565006814 21.885878613591643 54-55 20.722016196170436 27.18156361159464 28.755770831756607 23.340649360478317 56-57 22.012412018466662 27.752970559297662 27.64701430409445 22.587603118141224 58-59 21.52507095553453 27.663197729422894 28.80605487228004 22.005676442762535 60-61 21.497767350336787 28.604404752894872 28.649814576553396 21.24801332021494 62-63 21.09286308938167 28.683871944297284 28.097328388708092 22.125936577612958 64-65 21.066374025580867 28.672519488382655 28.203284643911296 22.05782184212518 66-67 20.91800052976123 28.561698263139974 27.95625685851591 22.56404434858289 68-69 21.310731042833357 27.60708339639776 28.30709853186015 22.775087028908732 70-71 21.515059785076435 27.678976842742546 27.921144240956565 22.88481913122446 72-73 21.079915241410625 27.76600575147571 27.87195398819434 23.282125018919327 74-75 20.712880278492506 27.610867261994855 27.724383229907673 23.951869229604963 76-77 21.06477977902225 27.013016497654004 28.155743907976387 23.76645981534736 78-79 20.993680705339237 27.47947175237447 28.160593332576532 23.366254209709766 80-81 21.017975402081362 27.57615894039735 27.59886471144749 23.807000946073796 82-83 20.65168029064487 27.630184680593402 27.864819860732666 23.853315168029066 84-85 20.935690222945606 27.69219122601158 27.116847723229494 24.255270827813316 86-87 21.327480511617345 28.24869446756982 26.451222281086807 23.972602739726025 88-89 21.19548758328286 27.53634161114476 26.033464566929133 25.234706238643245 90-91 21.45506851389204 27.030812324929972 26.659853130441363 24.854266030736618 92-93 21.341509576803695 28.011204481792717 26.917253387841622 23.730032553561966 94-95 22.268064650440973 26.730761951625727 26.303039479162727 24.69813391877058 96-97 21.794920322495173 26.961656383663275 26.1705590673379 25.072864226503654 98-99 21.461290933181903 27.40488358886996 25.606662880938863 25.527162597009273 100-101 21.547024079963652 27.570801150991976 25.62471603816447 25.2574587308799 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 39.0 1 24.5 2 17.5 3 22.0 4 16.5 5 14.0 6 16.0 7 30.5 8 39.0 9 37.5 10 29.5 11 18.0 12 19.0 13 19.5 14 20.5 15 23.0 16 30.5 17 35.0 18 29.5 19 26.5 20 31.5 21 34.0 22 45.0 23 49.5 24 42.5 25 50.5 26 66.5 27 81.5 28 92.0 29 103.5 30 120.0 31 130.0 32 163.5 33 203.0 34 238.5 35 284.5 36 345.5 37 379.0 38 372.0 39 382.0 40 379.5 41 369.5 42 389.5 43 433.5 44 468.5 45 492.5 46 499.0 47 533.5 48 638.0 49 612.5 50 543.0 51 498.0 52 429.5 53 411.5 54 388.5 55 361.5 56 319.0 57 281.5 58 260.0 59 233.5 60 199.5 61 165.0 62 143.0 63 115.5 64 81.5 65 70.5 66 52.0 67 34.0 68 26.0 69 14.5 70 10.0 71 10.5 72 12.5 73 12.5 74 5.5 75 4.5 76 4.5 77 2.5 78 2.0 79 2.0 80 1.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015135462388375966 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.007567731194187983 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0037838655970939914 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.007567731194187983 56-57 0.007567731194187983 58-59 0.011351596791281972 60-61 0.007567731194187983 62-63 0.007567731194187983 64-65 0.007567731194187983 66-67 0.0037838655970939914 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0037838655970939914 80-81 0.011351596791281972 82-83 0.015135462388375966 84-85 0.03405479037384592 86-87 0.007567731194187983 88-89 0.04540638716512789 90-91 0.03783865597093991 92-93 0.03783865597093991 94-95 0.03405479037384592 96-97 0.03405479037384592 98-99 0.04919025276222188 100-101 0.06054184955350386 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 13214.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.43862570001514 #Duplication Level Percentage of deduplicated Percentage of total 1 93.74015058658729 81.02769789617072 2 4.062335843109787 7.022854548206448 3 0.9718087900542812 2.520054487664598 4 0.4465067413762913 1.5438171636143483 5 0.21012081947119596 0.908127743302558 6 0.13132551216949745 0.6810958074769184 7 0.10506040973559798 0.6356894203117905 8 0.07004027315706532 0.4843347964280309 9 0.026265102433899495 0.20432874224307554 >10 0.20136578532656277 3.011957015286817 >50 0.03502013657853266 1.9600423792946873 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 78 0.5902830331466626 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 66 0.4994702588164069 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 61 0.46163160284546695 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 54 0.4086574844861511 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 41 0.31027697896170725 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 35 0.2648705917965794 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30 0.2270319358256395 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 23 0.1740578174663236 RNA PCR Primer, Index 33 (95% over 22bp) GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 22 0.1664900862721356 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21 0.15892235507794764 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20 0.15135462388375964 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 18 0.13621916149538368 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18 0.13621916149538368 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.12108369910700773 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 16 0.12108369910700773 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 14 0.10594823671863177 RNA PCR Primer, Index 33 (95% over 24bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.015135462388375966 0.0 24-25 0.0 0.0 0.0 0.015135462388375966 0.0 26-27 0.0 0.0 0.0 0.03405479037384592 0.0 28-29 0.0 0.0 0.0 0.09459663992734978 0.0 30-31 0.0 0.0 0.0 0.23459966701982748 0.0 32-33 0.0 0.0 0.0 0.45028000605418494 0.0 34-35 0.0 0.0 0.0 0.7000151354623885 0.0 36-37 0.0 0.0 0.0 0.9270470712880279 0.0 38-39 0.0 0.0 0.0 1.1124564855456334 0.0 40-41 0.0 0.0 0.0 1.267594975026487 0.0 42-43 0.0 0.0 0.0 1.4340850612986227 0.0 44-45 0.0 0.0 0.0 1.6081428787649463 0.0 46-47 0.0 0.0 0.0 1.7670652338428938 0.0 48-49 0.0 0.0 0.0 1.9865294384743453 0.0 50-51 0.0 0.0 0.0 2.217345239897079 0.0 52-53 0.0 0.0 0.0 2.4027546541546845 0.0 54-55 0.0 0.0 0.0 2.690328439533828 0.0 56-57 0.0 0.0 0.0 3.1216891176025428 0.0 58-59 0.0 0.0 0.0 3.534130467685788 0.0 60-61 0.0 0.0 0.0 3.9465718177690325 0.0 62-63 0.0 0.0 0.0 4.207658543968519 0.0 64-65 0.0 0.0 0.0 4.650370818828515 0.0 66-67 0.0 0.0 0.0 5.123354018465264 0.0 68-69 0.0 0.0 0.0 5.592553352504918 0.0 70-71 0.0 0.0 0.0 6.250945966399273 0.0 72-73 0.0 0.0 0.0 7.1136673225367035 0.0 74-75 0.0 0.0 0.0 7.885575904343877 0.0 76-77 0.0 0.0 0.0 8.490994399878916 0.0 78-79 0.0 0.0 0.0 8.771000454063872 0.0 80-81 0.0 0.0 0.0 8.963977599515665 0.0 82-83 0.0 0.0 0.0 9.134251551384896 0.0 84-85 0.0 0.0 0.0 9.304525503254125 0.0 86-87 0.0 0.0 0.0 9.448312395943695 0.0 88-89 0.0 0.0 0.0 9.686695928560617 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGCTTG 30 0.0038514815 31.717361 54-55 GCCGTCT 40 4.4337497E-4 29.622396 46-47 CGTCTTC 35 0.0081818225 27.18631 48-49 ACATGGG 70 0.0081869615 27.083334 3 GTACATG 70 0.0081869615 27.083334 1 ATGCCGT 35 0.008335288 27.083334 44-45 AGTACAT 55 9.2749135E-5 25.852272 12-13 GAGTACT 80 1.7814145E-6 23.697918 6 GTACTTT 85 3.0174506E-6 22.30392 8 TACATGG 85 0.0017514906 22.30392 2 AGTACTT 90 0.002566244 21.064814 7 GGTATCA 140 4.5789583E-4 20.3125 1 TACTTTT 95 0.0036795316 19.95614 9 GTATCAA 190 1.7245095E-4 17.461622 1 ACTTTTT 110 2.7989663E-5 17.234848 16-17 TATCAAC 205 3.082183E-4 16.183945 2 AGAGTAC 185 4.474714E-8 15.371621 10-11 ATCAACG 220 5.2771927E-4 15.080493 3 TCAACGC 225 6.2596047E-4 14.74537 4 AACGCAG 230 7.3956285E-4 14.424819 6 >>END_MODULE