FastQCFastQC Report
Thu 26 May 2016
SRR936739_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936739_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654434
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45130.6896035352686444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35290.5392445991497996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21450.3277641442834571No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9910.1514285626969259No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9030.13798182857247637No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8370.12789677797913923No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6730.10283695529266512No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33250.038.728251
GTATCAA52750.035.6716271
TATTCCG801.0731152E-535.6432955
ATACGGC650.005829792529.2435463
ATCAACG64400.028.925683
TATCAAC64950.028.5322272
TCAACGC65300.028.4564114
CAACGCA65850.028.2187395
AACGCAG67100.027.763886
GTACACG700.00837423327.1526431
TCGGTAA350.00838349327.14849512-13
GTACATG42500.025.715151
GTATAGG1501.0556196E-625.3424681
ACGCAGA74200.025.039357
GAGTACT39600.025.01466212-13
CGCAGAG74700.024.8717488
TACATGG43450.024.8248312
GTAGGGA1951.5599653E-824.3696213
ACATGGG43050.024.284713
ATGGGAG9650.023.6390765
Produced by FastQC (version 0.11.5)