##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936739_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 654434 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.4502440276636 31.0 31.0 34.0 30.0 34.0 2 31.484803662401404 31.0 31.0 34.0 28.0 34.0 3 31.484656970756408 31.0 31.0 34.0 28.0 34.0 4 35.15849573830211 37.0 35.0 37.0 32.0 37.0 5 35.17358511324289 37.0 35.0 37.0 33.0 37.0 6 35.1478529538502 37.0 35.0 37.0 32.0 37.0 7 35.0949247746908 37.0 35.0 37.0 32.0 37.0 8 35.05954458356381 37.0 35.0 37.0 32.0 37.0 9 36.553038197893144 39.0 37.0 39.0 32.0 39.0 10-11 36.44853415317664 39.0 37.0 39.0 32.0 39.0 12-13 36.33906474908089 39.0 36.0 39.0 32.0 39.0 14-15 37.398670454163444 40.0 37.0 41.0 32.0 41.0 16-17 37.327505141847766 40.0 37.0 41.0 32.0 41.0 18-19 37.350771047959 40.0 37.0 41.0 32.0 41.0 20-21 37.24321092730512 39.5 37.0 41.0 31.0 41.0 22-23 37.29936785069235 40.0 37.0 41.0 31.5 41.0 24-25 37.311343084252954 40.0 37.0 41.0 31.0 41.0 26-27 37.20901649364183 39.5 36.5 41.0 31.0 41.0 28-29 37.047528092978055 39.0 36.0 41.0 31.0 41.0 30-31 36.7840232934108 39.0 36.0 41.0 30.0 41.0 32-33 36.49529364305644 39.0 35.0 41.0 30.0 41.0 34-35 36.24642744722921 39.0 35.0 40.0 30.0 41.0 36-37 36.01660518860572 38.0 35.0 40.0 29.5 41.0 38-39 35.77958969124465 38.0 35.0 40.0 28.5 41.0 40-41 35.51394869459716 38.0 35.0 40.0 27.0 41.0 42-43 35.22297588450478 38.0 34.5 40.0 26.5 41.0 44-45 34.843322626880635 38.0 34.0 40.0 25.0 41.0 46-47 34.52326361405429 38.0 34.0 40.0 23.5 41.0 48-49 34.26189195549131 38.0 34.0 40.0 22.5 41.0 50-51 33.223296008459215 37.0 32.0 39.5 20.0 40.5 52-53 33.30087755220542 37.5 33.0 39.5 20.0 40.5 54-55 33.70963382098118 38.0 33.0 40.0 19.5 41.0 56-57 33.579489299150104 38.0 33.0 40.0 18.5 41.0 58-59 33.3338571956836 37.5 33.0 40.0 17.5 41.0 60-61 33.04190491325328 37.0 33.0 40.0 14.0 41.0 62-63 32.69000464523542 36.5 32.0 40.0 12.0 41.0 64-65 32.30423617966059 36.0 32.0 39.0 8.0 41.0 66-67 31.82361857727441 35.5 31.0 39.0 7.0 41.0 68-69 31.44324255769107 35.0 31.0 39.0 4.0 40.0 70-71 31.036246588655235 35.0 30.5 38.0 2.0 40.0 72-73 30.65872417997842 35.0 30.0 37.0 2.0 39.5 74-75 30.246457243969598 35.0 30.0 37.0 2.0 39.0 76-77 29.783476255818005 34.5 29.0 36.0 2.0 39.0 78-79 29.303756375738423 34.0 29.0 36.0 2.0 38.5 80-81 28.88287588970011 34.0 29.0 35.0 2.0 37.0 82-83 28.47556514484272 34.0 28.0 35.0 2.0 37.0 84-85 28.133184553369784 34.0 27.0 35.0 2.0 36.0 86-87 27.79862828031551 34.0 27.0 35.0 2.0 36.0 88-89 27.38788250610451 34.0 26.0 35.0 2.0 36.0 90-91 26.97347784497749 33.0 25.5 35.0 2.0 35.0 92-93 26.50338918821455 33.0 25.0 35.0 2.0 35.0 94-95 25.968889146957522 33.0 23.5 35.0 2.0 35.0 96-97 25.39447675395838 32.5 19.5 35.0 2.0 35.0 98-99 24.671001201037843 32.0 9.5 35.0 2.0 35.0 100-101 22.07909659339215 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6198811733011631 1101 2 0.5937818954221861 1101 3 0.6924040294442371 1101 4 0.6760924032032776 1101 5 0.6266428510084694 1101 6 0.6213736828906349 1101 7 0.7551938149754704 1101 8 0.6987385889837512 1101 9 0.7802708335566422 1101 10-11 0.898968337557875 1101 12-13 0.9473118384667956 1101 14-15 1.0608117779878796 1101 16-17 1.0367167024968609 1101 18-19 0.9627995644945813 1101 20-21 1.0008682591366735 1101 22-23 0.891548900190422 1101 24-25 0.9935174771561179 1101 26-27 0.8473999443762423 1101 28-29 0.8620909868362219 1101 30-31 1.0337050347876726 1101 32-33 0.7066587588080679 1101 34-35 0.6712290641335628 1101 36-37 0.5858837695740036 1101 38-39 0.578543795550857 1101 40-41 0.7355743279386999 1101 42-43 0.7688481357897174 1101 44-45 0.7301984969038315 1101 46-47 0.9232496837015987 1101 48-49 0.8641166354765559 1101 50-51 0.7650469838419411 1101 52-53 1.0029645464864743 1101 54-55 1.0301905646750171 1101 56-57 0.7665903828155152 1101 58-59 0.8518326608781379 1101 60-61 0.8979313494901433 1101 62-63 1.13586952115039 1101 64-65 0.9545650576656435 1101 66-67 0.9568556241196688 1101 68-69 0.872151725184267 1101 70-71 0.9388898184898835 1101 72-73 0.7577916783007055 1101 74-75 0.8244271600677138 1101 76-77 0.7129327398621257 1101 78-79 0.8433532332777887 1101 80-81 0.9136291768883886 1101 82-83 0.7395377402821879 1101 84-85 0.7576226280228369 1101 86-87 0.8131745084486468 1101 88-89 1.0515321018358144 1101 90-91 1.1493505291173065 1101 92-93 0.8146554979534848 1101 94-95 1.0382113558090538 1101 96-97 1.0473740828331728 1101 98-99 1.2784042158078854 1101 100-101 1.2023852200160405 1102 1 0.3092259273339586 1102 2 0.46016082267632896 1102 3 0.41294018017969236 1102 4 0.5169747300041507 1102 5 0.4773138726500292 1102 6 0.5282649862724043 1102 7 0.46479253514610974 1102 8 0.5145553689686437 1102 9 0.48722101797650197 1102 10-11 0.6399712038423715 1102 12-13 0.6388951829097564 1102 14-15 0.6435307487917754 1102 16-17 0.6077050151662817 1102 18-19 0.40037844286636215 1102 20-21 0.27207209862474 1102 22-23 0.2825439897340374 1102 24-25 0.5667054965313127 1102 26-27 0.12974285421048393 1102 28-29 0.027115094732941714 1102 30-31 0.3446024706851034 1102 32-33 0.3885545060417783 1102 34-35 0.45986391282040273 1102 36-37 0.2201747529984388 1102 38-39 0.1144862254490917 1102 40-41 0.41190581707348173 1102 42-43 0.09504797581104896 1102 44-45 0.4232360918911624 1102 46-47 0.5375499992150878 1102 48-49 0.8507695262022352 1102 50-51 0.8298700074387924 1102 52-53 0.9098686259425506 1102 54-55 0.8448002833791861 1102 56-57 1.04064106494679 1102 58-59 0.9837422951491277 1102 60-61 1.034151520134067 1102 62-63 1.0502320505909069 1102 64-65 1.000265630922538 1102 66-67 0.8364966719799511 1102 68-69 0.9773699183140714 1102 70-71 0.9852569695364117 1102 72-73 0.6508970420299853 1102 74-75 0.725153729858409 1102 76-77 0.6238318422040336 1102 78-79 0.6713850512576158 1102 80-81 0.7552564043691703 1102 82-83 0.795254200309742 1102 84-85 0.5707308802558728 1102 86-87 0.6229370956592426 1102 88-89 0.7367490729063384 1102 90-91 0.6610078636949126 1102 92-93 0.34495423589953944 1102 94-95 0.2934645442728119 1102 96-97 0.4436862412120526 1102 98-99 0.6977133079289359 1102 100-101 0.467688735102918 1103 1 0.32209150091381744 1103 2 0.25531606426014264 1103 3 0.37791343443866054 1103 4 0.4161912542206778 1103 5 0.5234531830750555 1103 6 0.34833737420193245 1103 7 0.3331395320645356 1103 8 0.34932919302818277 1103 9 0.2607252914807745 1103 10-11 0.530953964467983 1103 12-13 3.057300345901126E-4 1103 14-15 0.47026340766728936 1103 16-17 0.5509371798270237 1103 18-19 0.5943352136088507 1103 20-21 0.7224354918452818 1103 22-23 0.33370612125330723 1103 24-25 0.5896515980488459 1103 26-27 0.3890566224528271 1103 28-29 0.4011637312815779 1103 30-31 0.43430972110664356 1103 32-33 0.3847694022566728 1103 34-35 0.7470637500202386 1103 36-37 0.7046883875120713 1103 38-39 0.679239263416413 1103 40-41 0.7269481160443547 1103 42-43 0.5664314864802762 1103 44-45 0.9598646749483137 1103 46-47 1.0890471792836962 1103 48-49 1.22236380493937 1103 50-51 1.1248864328123602 1103 52-53 1.2246798068251508 1103 54-55 1.4541751016825728 1103 56-57 1.1853435185778167 1103 58-59 1.0837212183423333 1103 60-61 1.1943665035633302 1103 62-63 1.4030607034195626 1103 64-65 1.291317436260055 1103 66-67 1.228376287073857 1103 68-69 1.299166243681828 1103 70-71 1.0300735286386846 1103 72-73 0.920264447111677 1103 74-75 0.9828344089676584 1103 76-77 1.1389628091207165 1103 78-79 1.2028926970509737 1103 80-81 1.3427593115149357 1103 82-83 1.1005633752617712 1103 84-85 1.012188232427512 1103 86-87 0.9179898603548651 1103 88-89 1.0078731208161003 1103 90-91 1.001072693045458 1103 92-93 1.078525228399105 1103 94-95 1.2092874118813945 1103 96-97 1.1868973290659994 1103 98-99 1.0652339486638631 1103 100-101 0.78008335106896 1104 1 0.5359367031604876 1104 2 0.5686714171194005 1104 3 0.6349762095308371 1104 4 0.5233128452650959 1104 5 0.5917750813234619 1104 6 0.5850235813750473 1104 7 0.6816447076252956 1104 8 0.5878734496018367 1104 9 0.7381279352821295 1104 10-11 0.8264880230360347 1104 12-13 0.7655255728842079 1104 14-15 0.7665396530707724 1104 16-17 0.7770738831685904 1104 18-19 0.6441938851946603 1104 20-21 0.4930857848313579 1104 22-23 0.6298778589730532 1104 24-25 0.6713225300872523 1104 26-27 0.5282660218252957 1104 28-29 0.4942815965055516 1104 30-31 0.5827404045625926 1104 32-33 0.6544894553368508 1104 34-35 0.7293873689998094 1104 36-37 0.41967800965603175 1104 38-39 0.38931823962997925 1104 40-41 0.4794845539850243 1104 42-43 0.44313520476644186 1104 44-45 0.419575443857525 1104 46-47 0.4779366757753962 1104 48-49 0.7713153255261673 1104 50-51 0.46765857203603645 1104 52-53 0.6569159917600089 1104 54-55 0.7004571800730588 1104 56-57 0.5957764841899333 1104 58-59 0.4126245195699383 1104 60-61 0.44287544626153164 1104 62-63 0.6687125518688362 1104 64-65 0.7339864652864598 1104 66-67 0.6068147113896316 1104 68-69 0.6067767119491627 1104 70-71 0.8359186069343814 1104 72-73 0.6018596110793695 1104 74-75 0.7988799232791344 1104 76-77 0.6773080150130042 1104 78-79 0.8240480902550615 1104 80-81 0.9180552748448889 1104 82-83 0.8231738556811656 1104 84-85 0.6841264610951825 1104 86-87 0.6355092368085522 1104 88-89 0.5488574495243626 1104 90-91 0.536442771766481 1104 92-93 0.3911915596690143 1104 94-95 0.491223015704918 1104 96-97 0.945447515843103 1104 98-99 0.9683240342872494 1104 100-101 1.1256984638521956 1105 1 0.4297299666736123 1105 2 0.2539349709025309 1105 3 0.34303326061523975 1105 4 0.4241138237870814 1105 5 0.324925393549222 1105 6 0.4720742591912668 1105 7 0.6422073577664165 1105 8 0.7695869811920346 1105 9 0.8242424440390224 1105 10-11 0.7497051342954819 1105 12-13 0.4742306237246581 1105 14-15 0.5115233726063124 1105 16-17 0.340388541685428 1105 18-19 0.40791708002828386 1105 20-21 0.47540894442734327 1105 22-23 0.3294135015008024 1105 24-25 0.12095558434304365 1105 26-27 0.023468705984278415 1105 28-29 0.14958172627492416 1105 30-31 0.272377321405159 1105 32-33 -0.01934854980648737 1105 34-35 0.3194178292236316 1105 36-37 0.23701704312839666 1105 38-39 0.08048113236865362 1105 40-41 0.18609355855574705 1105 42-43 0.21981966530876917 1105 44-45 0.6379094322494367 1105 46-47 0.9109606068722655 1105 48-49 0.9080754622752991 1105 50-51 0.8732797369786667 1105 52-53 0.7826952855842748 1105 54-55 0.8228605222270957 1105 56-57 0.7757438046728211 1105 58-59 0.8615917858410782 1105 60-61 0.9736448464424541 1105 62-63 1.0672324018378418 1105 64-65 1.0634549530159738 1105 66-67 0.9692460572978305 1105 68-69 1.232542029308096 1105 70-71 1.124140004309858 1105 72-73 0.7986139370071541 1105 74-75 0.7787364485096191 1105 76-77 0.844533457939896 1105 78-79 1.0130429367511162 1105 80-81 1.256051100540578 1105 82-83 1.0233583450234747 1105 84-85 0.9279929104631073 1105 86-87 0.8427166882128034 1105 88-89 0.7404591185684382 1105 90-91 0.7665839086545212 1105 92-93 0.8782129292609717 1105 94-95 1.1592311406900961 1105 96-97 1.3620520648860932 1105 98-99 1.4250008035383495 1105 100-101 1.4052984997674756 1106 1 0.3045892507008148 1106 2 0.32467337137615715 1106 3 0.3728681497366395 1106 4 0.36501994156526507 1106 5 0.4315269740362808 1106 6 0.3643866678887875 1106 7 0.42058668210633954 1106 8 0.5678148597723052 1106 9 0.7746632577155594 1106 10-11 0.6585937915020565 1106 12-13 0.6288109208820387 1106 14-15 0.5137332494713007 1106 16-17 0.779305087649746 1106 18-19 0.5397358316495442 1106 20-21 0.3191772902529024 1106 22-23 0.5128885127022329 1106 24-25 0.36832278383764105 1106 26-27 0.5204570428487045 1106 28-29 0.4671610764308767 1106 30-31 0.4742232915603495 1106 32-33 0.416206947828492 1106 34-35 0.5298240469148112 1106 36-37 0.7155040997412101 1106 38-39 0.7870478363941018 1106 40-41 0.6155605692706345 1106 42-43 0.8128585221551603 1106 44-45 0.7717388553232567 1106 46-47 0.7099791013261694 1106 48-49 0.7816973145505202 1106 50-51 0.40633666504502486 1106 52-53 0.46344409935017694 1106 54-55 0.5834441630566332 1106 56-57 0.7177011809203364 1106 58-59 0.7891796875564765 1106 60-61 0.6011636158122329 1106 62-63 0.7090976843682029 1106 64-65 0.7955360848502373 1106 66-67 0.7597831175720557 1106 68-69 0.7661916089908836 1106 70-71 0.5687398000157096 1106 72-73 0.5650539687310818 1106 74-75 0.7648882591689095 1106 76-77 0.8223651845977997 1106 78-79 0.649069204560142 1106 80-81 0.8675049773509578 1106 82-83 0.6577655258511044 1106 84-85 0.73815635819372 1106 86-87 0.7437432476933097 1106 88-89 0.7368566655492685 1106 90-91 0.7613816065598229 1106 92-93 0.7584327626731202 1106 94-95 0.5012395284170523 1106 96-97 0.6354469098720159 1106 98-99 0.9676655141443895 1106 100-101 0.6837007854789263 1107 1 0.33783201580060407 1107 2 0.46768201993856096 1107 3 0.29140835874747495 1107 4 0.5278925969977166 1107 5 0.4933573908847819 1107 6 0.5110696649934141 1107 7 0.4504601212121031 1107 8 0.3286349480880588 1107 9 0.43823746500888205 1107 10-11 0.602596344306157 1107 12-13 0.5371891863830527 1107 14-15 0.19738967207300107 1107 16-17 0.3741929097418293 1107 18-19 0.384798455125015 1107 20-21 0.18133760071893334 1107 22-23 0.0701435913276569 1107 24-25 0.30086118148381047 1107 26-27 0.21609615894028167 1107 28-29 0.20536491596084971 1107 30-31 0.14857887276907888 1107 32-33 0.24649709466165604 1107 34-35 0.3076573544385397 1107 36-37 0.24302182624391833 1107 38-39 0.2969422985924197 1107 40-41 0.31460655259294157 1107 42-43 0.2827152554106007 1107 44-45 0.10223547895827778 1107 46-47 0.35292439905163775 1107 48-49 0.36374838001774634 1107 50-51 0.36218817068325393 1107 52-53 0.19583373984590935 1107 54-55 0.44573117519653493 1107 56-57 0.20073019560364713 1107 58-59 0.07102521475275836 1107 60-61 0.41788964455943756 1107 62-63 0.44974170428382365 1107 64-65 0.4620901278067997 1107 66-67 0.43873515394950857 1107 68-69 0.6356419583590203 1107 70-71 0.28848508272468365 1107 72-73 0.20170407032705384 1107 74-75 0.3826621576855622 1107 76-77 0.03118831375373432 1107 78-79 0.1000192324814364 1107 80-81 0.3065473942557766 1107 82-83 0.31283898935867427 1107 84-85 0.29673711174019246 1107 86-87 0.14620172943184429 1107 88-89 0.15228564456840843 1107 90-91 0.20018704440796142 1107 92-93 0.3947614174298302 1107 94-95 0.2566489859931096 1107 96-97 0.2916137618985388 1107 98-99 0.551157224570062 1107 100-101 0.3474134260825892 1108 1 0.07135970470542574 1108 2 0.11064948229062566 1108 3 0.0747605188676026 1108 4 0.21899575651583802 1108 5 0.26417339117338656 1108 6 0.14348961732895305 1108 7 0.3784358245109658 1108 8 0.17281904926118585 1108 9 0.2892104829540827 1108 10-11 0.32852905939937216 1108 12-13 -0.02278258471027783 1108 14-15 -0.05969179267355429 1108 16-17 0.19574428206802708 1108 18-19 0.44746008929011083 1108 20-21 0.503241834378521 1108 22-23 0.3920162591171703 1108 24-25 0.13955169391061872 1108 26-27 0.29154197048753616 1108 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-0.8548100724602143 2316 94-95 -0.43469122078642286 2316 96-97 -0.987692728697283 2316 98-99 -1.951864671936466 2316 100-101 -3.214280506790402 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1512.0 3 599.0 4 552.0 5 621.0 6 731.0 7 937.0 8 1138.0 9 1474.0 10 1949.0 11 2430.0 12 3221.0 13 4096.0 14 4637.0 15 6141.0 16 7694.0 17 10018.0 18 7667.0 19 5266.0 20 4901.0 21 5344.0 22 5629.0 23 6219.0 24 7034.0 25 7907.0 26 9182.0 27 10676.0 28 12623.0 29 15303.0 30 18047.0 31 22629.0 32 27864.0 33 35413.0 34 46825.0 35 63198.0 36 87233.0 37 110110.0 38 84829.0 39 12782.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.28930191900066 18.277957854423697 14.684167977125426 21.74857224945022 2 15.806814934355407 20.051592632156453 40.38269629575293 23.75889613773521 3 19.055726040125684 25.628262809775897 30.047345127333635 25.268666022764783 4 12.511367370488397 16.639079300304914 37.152311301649895 33.697242027556804 5 13.18171812228276 37.679391460141005 33.713823613939184 15.425066803637053 6 31.203079943367207 35.97558551643788 17.5550961870717 15.266238353123212 7 28.03847288208893 33.88718838967394 20.464773687580514 17.60956504065662 8 27.096593723058866 33.08371700570464 19.698442687602032 20.121246583634463 9 27.445102151885575 16.350263864343763 20.473488207128923 35.73114577664174 10-11 25.05054767304886 26.62939325210542 26.557307874887915 21.7627511999578 12-13 26.73762090564105 24.34385163048255 27.28861815027267 21.629909313603722 14-15 23.2568964054543 25.372917588594916 26.741231077064416 24.628954928886372 16-17 21.58317589008933 28.750664930205254 27.317549265978197 22.348609913727216 18-19 21.453408573323838 26.263561081034815 31.04688947241319 21.236140873228155 20-21 22.538865649004354 25.22383678262146 31.120040119379617 21.117257448994568 22-23 21.975430711381538 25.30658122864782 31.307313248878472 21.41067481109217 24-25 21.695387149665883 25.71335139537691 31.359573723546823 21.231687731410386 26-27 21.178696156100035 25.85217744085852 31.27486432777401 21.694262075267428 28-29 21.029425472126253 26.21498304639283 31.21625896004633 21.539332521434584 30-31 21.19379356924779 25.823709005559138 31.141029128171905 21.84146829702117 32-33 20.861201394205278 26.25870423258351 30.922889323877676 21.957205049333535 34-35 21.799753680279448 26.057937087620754 30.663901936635323 21.47840729546448 36-37 21.884583707067666 25.69598628426832 30.66251445912556 21.756915549538455 38-39 21.34091648801329 26.047280623073714 30.648973997338153 21.962828891574844 40-41 21.54355232184565 26.084176120887708 30.686609858570606 21.68566169869603 42-43 21.445811484922782 26.255148686344814 30.171397485844214 22.127642342888183 44-45 21.48055276047886 26.34979283324509 30.21364572995051 21.956008676325535 46-47 22.061352674352243 26.785620147013546 29.316756044768766 21.836271133865452 48-49 21.981722900467663 26.98588303787062 28.71235916082121 22.320034900840508 50-51 21.83686473700135 27.279825920594607 28.751295051756227 22.132014290647817 52-53 22.290009497009933 27.092837665309478 28.486751174519593 22.130401663161 54-55 21.650158006369153 27.2253073025226 28.63422289594807 22.490311795160174 56-57 21.920049020057274 27.32341013346047 28.28249488865051 22.47404595783175 58-59 22.10237500725858 26.836038618699938 28.33015993325163 22.731426440789857 60-61 21.672353644988913 26.97186359198006 29.09939962012105 22.25638314290997 62-63 21.742120081139003 27.058023554759274 29.02178605127343 22.17807031282829 64-65 21.335038632826556 27.238721300428388 28.8826697410955 22.543570325649554 66-67 21.509252877339964 27.283036111361575 28.56311044614625 22.644600565152206 68-69 21.734175444604023 26.986415184908036 28.83301141405749 22.44639795643045 70-71 21.68777113153821 27.006249163911555 28.502073499340685 22.803906205209547 72-73 21.633385397403725 27.206864510134153 28.450823906272447 22.708926186189675 74-75 21.54268734466282 27.83076692621159 28.07793073299278 22.548614996132812 76-77 21.70265191030935 27.39043740967387 28.23288264844056 22.67402803157622 78-79 21.668740979034666 27.50331788047068 28.18248954179612 22.645451598698536 80-81 21.863008838188804 27.798007851749666 27.77024991320865 22.568733396852878 82-83 21.870215207222856 27.590983204923482 27.84668176651396 22.692119821339706 84-85 21.697922167041234 27.606193686034864 27.734114408809873 22.961769738114032 86-87 21.9031214848144 27.611477233824033 27.553858756785836 22.931542524575733 88-89 21.710589662977675 27.578180284247484 27.377045923131828 23.334184129643017 90-91 21.6739250397822 27.516979090076692 27.72181348758077 23.08728238256034 92-93 21.87863712262763 27.316314560717736 27.777357466824093 23.027690849830538 94-95 21.56581367431632 27.300416371523518 27.88420760655527 23.24956234760489 96-97 21.673599175893667 27.180225116138317 27.86563031830954 23.280545389658474 98-99 21.73373902938748 27.497477141371824 27.27707103758295 23.491712791657747 100-101 22.03416011329608 27.295362018285203 27.09057874597392 23.579899122444797 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 263.0 1 207.0 2 257.0 3 338.0 4 285.5 5 277.5 6 413.5 7 750.5 8 1238.0 9 1500.0 10 1325.0 11 1100.5 12 1079.5 13 1149.5 14 1220.0 15 1298.5 16 1396.5 17 1445.0 18 1496.0 19 1655.5 20 1850.5 21 2042.5 22 2292.0 23 2715.0 24 3167.0 25 3738.5 26 4475.5 27 5298.5 28 6190.5 29 7200.0 30 8412.0 31 9929.0 32 12031.0 33 13147.5 34 13252.5 35 13946.0 36 15296.0 37 16580.0 38 17307.5 39 17596.0 40 17762.5 41 18686.5 42 19315.0 43 19796.5 44 20540.5 45 21585.0 46 21946.0 47 22333.0 48 24521.0 49 24648.0 50 22510.0 51 21207.0 52 20854.0 53 21259.5 54 21288.0 55 19873.0 56 17950.5 57 16377.0 58 15701.0 59 14223.5 60 11730.5 61 9906.0 62 8583.0 63 6584.5 64 4699.5 65 3682.0 66 2688.5 67 2128.5 68 1663.0 69 1158.0 70 909.5 71 713.0 72 561.0 73 485.0 74 400.5 75 333.0 76 253.0 77 167.0 78 107.0 79 75.5 80 62.5 81 45.0 82 28.0 83 18.0 84 13.0 85 7.5 86 5.5 87 4.0 88 2.0 89 2.0 90 2.5 91 3.0 92 1.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012682715140105801 2 0.011765892358893333 3 0.013752341718187013 4 0.022767765733442944 5 0.042479455529511 6 0.060510303560022856 7 0.007792993640305974 8 0.03514487327981126 9 0.04736917702931082 10-11 0.053252123208757496 12-13 0.03919417389683299 14-15 0.03583249036572061 16-17 0.03514487327981126 18-19 0.026282253061424073 20-21 0.05898226559133542 22-23 0.01230070564793394 24-25 0.003514487327981126 26-27 0.0011460284765155845 28-29 1.528037968687446E-4 30-31 0.0024448607498999135 32-33 0.0016808417655561905 34-35 0.0 36-37 4.5841139060623376E-4 38-39 1.528037968687446E-4 40-41 0.001298832273384329 42-43 0.0029796740389405195 44-45 0.0014516360702530736 46-47 6.876170859093507E-4 48-49 0.001298832273384329 50-51 0.003056075937374892 52-53 0.0026740664452030304 54-55 0.004584113906062338 56-57 0.0012224303749499568 58-59 0.005500936687274805 60-61 0.002292056953031169 62-63 2.2920569530311688E-4 64-65 5.348132890406061E-4 66-67 0.014821968296268225 68-69 0.04347268020915784 70-71 0.05294651561502 72-73 0.017114025249299395 74-75 0.032394404936173855 76-77 0.0317067878502645 78-79 0.06142712634123532 80-81 0.08663975282457818 82-83 0.05248810422441377 84-85 0.018718465116421213 86-87 0.019864493592936797 88-89 0.022156550545967966 90-91 0.037742537826579915 92-93 0.02391379420995853 94-95 0.013828743616621387 96-97 0.022844167631877316 98-99 0.06326077190366027 100-101 0.0874037718089219 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 654434.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.75049116948852 #Duplication Level Percentage of deduplicated Percentage of total 1 83.35917334037788 52.308290705912455 2 9.91744226469561 12.446487465093885 3 2.8248338109275206 5.3177912732363986 4 1.2401035863385723 3.1126843657515852 5 0.6455084876606211 2.025298732738885 6 0.3896645915287062 1.4670986705870657 7 0.2554949069037207 1.1222701629657852 8 0.20163900722923966 1.0122357394050268 9 0.1513461516760944 0.8547340818858147 >10 0.9167254456207389 10.587795868612249 >50 0.060650829039287486 2.649528522071239 >100 0.03350462866851074 4.229110835662631 >500 0.0031792713334578347 1.3033543191446606 >1k 7.336780000287311E-4 1.5633192569323744 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4513 0.6896035352686444 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3529 0.5392445991497996 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2145 0.3277641442834571 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 991 0.1514285626969259 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 903 0.13798182857247637 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 837 0.12789677797913923 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 673 0.10283695529266512 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 7.64018984343723E-5 0.0 20-21 0.0 0.0 0.0 2.292056953031169E-4 0.0 22-23 0.0 0.0 0.0 3.056075937374892E-4 0.0 24-25 0.0 0.0 0.0 7.64018984343723E-4 0.0 26-27 0.0 0.0 0.0 0.0016044398671218182 0.0 28-29 0.0 0.0 0.0 0.002826870242071775 0.0 30-31 0.0 0.0 0.0 0.0058065442810122945 0.0 32-33 0.0 0.0 0.0 0.009703041101165282 0.0 34-35 0.0 0.0 0.0 0.013828743616621387 0.0 36-37 0.0 0.0 0.0 0.01894767081172433 0.0 38-39 0.0 0.0 0.0 0.02788669292854589 0.0 40-41 0.0 0.0 0.0 0.04859160740426078 0.0 42-43 0.0 0.0 0.0 0.08045119905139403 0.0 44-45 0.0 0.0 0.0 0.11315121158130537 0.0 46-47 0.0 0.0 0.0 0.1792388537270374 0.0 48-49 0.0 0.0 0.0 0.24693093573989128 0.0 50-51 0.0 0.0 0.0 0.2971269830112738 0.0 52-53 0.0 0.0 0.0 0.35717887518069047 0.0 54-55 0.0 0.0 0.0 0.4146331028033384 0.0 56-57 0.0 0.0 0.0 0.5290831466580281 0.0 58-59 0.0 0.0 0.0 0.6775320353160135 0.0 60-61 0.0 0.0 0.0 0.837288404942286 0.0 62-63 0.0 0.0 0.0 0.9965863631779521 0.0 64-65 0.0 0.0 0.0 1.186597884584236 0.0 66-67 0.0 0.0 0.0 1.4363556905661992 0.0 68-69 0.0 0.0 0.0 1.7317254299134826 0.0 70-71 0.0 0.0 0.0 1.9802608055204955 0.0 72-73 0.0 0.0 0.0 2.1915884565899693 0.0 74-75 0.0 0.0 0.0 2.4042913418312617 0.0 76-77 0.0 0.0 0.0 2.6687183123126244 0.0 78-79 0.0 0.0 0.0 2.944681969457577 0.0 80-81 0.0 0.0 0.0 3.2968947212400335 0.0 82-83 0.0 0.0 0.0 3.6244296598281873 0.0 84-85 0.0 0.0 0.0 4.079708572598612 0.0 86-87 0.0 0.0 0.0 4.609632140139418 0.0 88-89 0.0 0.0 0.0 5.293734738720787 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3325 0.0 38.72825 1 GTATCAA 5275 0.0 35.671627 1 TATTCCG 80 1.0731152E-5 35.643295 5 ATACGGC 65 0.0058297925 29.243546 3 ATCAACG 6440 0.0 28.92568 3 TATCAAC 6495 0.0 28.532227 2 TCAACGC 6530 0.0 28.456411 4 CAACGCA 6585 0.0 28.218739 5 AACGCAG 6710 0.0 27.76388 6 GTACACG 70 0.008374233 27.152643 1 TCGGTAA 35 0.008383493 27.148495 12-13 GTACATG 4250 0.0 25.71515 1 GTATAGG 150 1.0556196E-6 25.342468 1 ACGCAGA 7420 0.0 25.03935 7 GAGTACT 3960 0.0 25.014662 12-13 CGCAGAG 7470 0.0 24.871748 8 TACATGG 4345 0.0 24.824831 2 GTAGGGA 195 1.5599653E-8 24.369621 3 ACATGGG 4305 0.0 24.28471 3 ATGGGAG 965 0.0 23.639076 5 >>END_MODULE