FastQCFastQC Report
Thu 26 May 2016
SRR936739_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936739_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654434
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52350.7999278766078779No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45580.6964797061277379No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28500.43549082107592213No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12330.18840708153916208No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12270.1874902587579496No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10920.1668617461806691No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC10410.15906875254036312No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA9490.14501080322843862No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8670.13248089188520157No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA8540.1304944425259079No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT6640.10146172112084641No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6620.10115611352710893No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAG3300.037.4220432
TAGTACG651.3280183E-436.536324
GGTATCA41150.034.168281
TAAGGTG4000.033.248055
GTAAGGT3900.032.8826874
GTATCAA61150.031.3824041
GGTAAGG4150.029.757293
CTATATG908.9424866E-426.3893571
AAGGTAA4900.025.2045291
ATCAACG76150.025.136413
TCAACGC76850.024.8456464
TATCAAC77200.024.7945292
CAACGCA77200.024.7330045
AACGCAG78800.024.228966
AAGGTGT6100.024.1360846
AGGTGTG6150.023.9398567
GAGTACT49600.023.22369812-13
GTGTAAC2303.61797E-922.7177931
AGTACGG1050.002195967822.6177185
GTACTTT52300.021.88853314-15
Produced by FastQC (version 0.11.5)