##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936739_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 654434 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.820924646335612 31.0 31.0 34.0 30.0 34.0 2 31.946850255335146 31.0 31.0 34.0 30.0 34.0 3 32.01149542963844 33.0 31.0 34.0 30.0 34.0 4 35.79357582277204 37.0 35.0 37.0 35.0 37.0 5 35.64245745178276 37.0 35.0 37.0 33.0 37.0 6 35.70360800325167 37.0 35.0 37.0 33.0 37.0 7 35.623208757491206 37.0 35.0 37.0 33.0 37.0 8 35.58908155749854 37.0 35.0 37.0 33.0 37.0 9 37.17150545356751 39.0 37.0 39.0 33.0 39.0 10-11 37.07388445588096 39.0 37.0 39.0 33.0 39.0 12-13 36.940327825265804 39.0 37.0 39.0 33.0 39.0 14-15 37.974988310509545 40.0 37.0 41.0 33.0 41.0 16-17 37.94717954751739 40.0 37.0 41.0 33.0 41.0 18-19 38.12260747455053 40.0 37.0 41.0 33.0 41.0 20-21 38.14079953058673 40.0 37.0 41.0 33.0 41.0 22-23 38.0366240140335 40.0 37.0 41.0 33.0 41.0 24-25 37.894398060003 40.0 37.0 41.0 33.0 41.0 26-27 37.64791331134996 39.5 37.0 41.0 32.0 41.0 28-29 37.630205643349825 40.0 37.0 41.0 32.0 41.0 30-31 37.4268833831983 39.0 36.0 41.0 32.0 41.0 32-33 37.1421923371952 39.0 35.5 41.0 31.5 41.0 34-35 36.897107271321474 39.0 35.0 41.0 31.0 41.0 36-37 36.6407918903969 39.0 35.0 41.0 30.5 41.0 38-39 36.3878389570224 39.0 35.0 40.0 30.0 41.0 40-41 36.09499200836142 38.5 35.0 40.0 30.0 41.0 42-43 35.74446926657234 38.0 35.0 40.0 28.5 41.0 44-45 35.31088314482438 38.0 34.5 40.0 27.0 41.0 46-47 34.76502443332712 38.0 34.0 40.0 25.0 41.0 48-49 34.20171476420846 38.0 33.5 40.0 23.0 41.0 50-51 33.82513973907224 38.0 33.0 40.0 21.5 41.0 52-53 33.54183233144977 38.0 33.0 40.0 20.0 41.0 54-55 33.184910166647825 37.0 33.0 40.0 18.5 41.0 56-57 32.83259732837841 37.0 32.0 40.0 16.5 41.0 58-59 32.425682345354915 36.0 31.5 39.0 13.5 40.5 60-61 32.019474079891936 36.0 31.0 39.0 10.0 40.0 62-63 31.69964962089378 35.0 31.0 39.0 8.0 40.0 64-65 31.391310659287264 35.0 30.5 38.5 7.0 40.0 66-67 30.937565896637402 35.0 30.0 38.0 4.0 40.0 68-69 30.45673742501154 34.5 29.0 37.5 2.0 40.0 70-71 29.92523768630603 34.0 29.0 37.0 2.0 39.0 72-73 29.378449927723803 34.0 28.0 36.0 2.0 39.0 74-75 28.871559240504006 34.0 27.5 36.0 2.0 38.5 76-77 27.490024204121426 31.5 26.0 34.5 2.0 36.5 78-79 28.26368663608553 33.0 27.0 35.0 2.0 37.0 80-81 28.107074204579835 33.0 27.0 35.0 2.0 37.0 82-83 27.696948813784125 33.0 26.0 35.0 2.0 36.0 84-85 27.245723021725645 33.0 26.0 35.0 2.0 36.0 86-87 26.819600601435745 33.0 25.0 35.0 2.0 35.0 88-89 26.323054731263962 32.0 24.5 34.5 2.0 35.0 90-91 25.749900677532036 32.0 23.5 34.0 2.0 35.0 92-93 25.128183285098267 31.5 20.0 34.0 2.0 35.0 94-95 24.371405672688155 31.0 16.5 34.0 2.0 35.0 96-97 23.572342665570552 31.0 4.5 34.0 2.0 35.0 98-99 22.361272488898805 30.5 2.0 34.0 2.0 35.0 100-101 18.927690187245773 24.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4939189103026429 1101 2 0.6148882485734113 1101 3 0.5457971426602732 1101 4 0.3282520230947341 1101 5 0.38476901372245464 1101 6 0.3776748648644528 1101 7 0.3953873103844998 1101 8 0.31809316536480026 1101 9 0.3998290424398121 1101 10-11 0.5411010588224556 1101 12-13 0.5903702649950233 1101 14-15 0.6243771180038848 1101 16-17 0.5900057311933367 1101 18-19 0.600439227413041 1101 20-21 0.5038809064510161 1101 22-23 0.5887070244314074 1101 24-25 0.6636799509946556 1101 26-27 0.5309342537054107 1101 28-29 0.6382031210630856 1101 30-31 0.95724160645225 1101 32-33 0.7474474541616161 1101 34-35 0.7534769950003124 1101 36-37 0.8543710977968502 1101 38-39 0.9889259928764957 1101 40-41 0.9533974587074354 1101 42-43 1.127059465079249 1101 44-45 1.106343649290416 1101 46-47 1.2598967351783443 1101 48-49 1.286358835942039 1101 50-51 1.3033987897764305 1101 52-53 1.3374316714124532 1101 54-55 1.2290098346365852 1101 56-57 1.4480086702703119 1101 58-59 1.380489428297782 1101 60-61 1.4462375921229835 1101 62-63 1.453737400418202 1101 64-65 1.4761687370427552 1101 66-67 1.498112130229515 1101 68-69 1.5946140811397989 1101 70-71 1.3158959826778975 1101 72-73 1.6637668332831872 1101 74-75 1.7058987700635875 1101 76-77 1.716529445822399 1101 78-79 1.6548781809154853 1101 80-81 1.8498584411856314 1101 82-83 1.9107638738610078 1101 84-85 1.630667680819137 1101 86-87 1.577822452079424 1101 88-89 1.3795468540884563 1101 90-91 1.62334880306123 1101 92-93 1.7532309381576248 1101 94-95 1.7101701252217723 1101 96-97 1.7701318655126101 1101 98-99 1.3072845267638407 1101 100-101 0.8197594449034931 1102 1 0.32173964531575194 1102 2 0.5273054818312026 1102 3 0.5158755766468524 1102 4 0.2796451706750531 1102 5 0.19561645628566993 1102 6 0.39094531769296736 1102 7 0.39430856532828784 1102 8 0.315507954504028 1102 9 0.4261910830566151 1102 10-11 0.520803765128278 1102 12-13 0.43521817416269215 1102 14-15 0.6817250271715594 1102 16-17 0.6012309011706733 1102 18-19 0.5681907494323255 1102 20-21 0.529398059719469 1102 22-23 0.3879454593067635 1102 24-25 0.5295911628330785 1102 26-27 0.6505560819060889 1102 28-29 0.611039520654252 1102 30-31 0.6691622392567567 1102 32-33 0.7226074328311256 1102 34-35 0.7526576598005619 1102 36-37 0.8337993962459436 1102 38-39 0.7057987431764516 1102 40-41 0.9359197890633837 1102 42-43 0.8845962379539785 1102 44-45 0.968320385725562 1102 46-47 1.0831603000363685 1102 48-49 1.2305373899126195 1102 50-51 1.157892635041513 1102 52-53 1.2123761232633186 1102 54-55 1.250873030654887 1102 56-57 1.506734729018035 1102 58-59 1.5861964451399864 1102 60-61 1.4592569673174083 1102 62-63 1.2150447816562568 1102 64-65 1.3833899964303953 1102 66-67 1.2941754329002677 1102 68-69 1.45893772687592 1102 70-71 1.140516588819299 1102 72-73 1.2395978335942566 1102 74-75 1.2242740977976716 1102 76-77 1.3858940860925877 1102 78-79 1.311937239707646 1102 80-81 1.166094609696497 1102 82-83 1.4164142315910837 1102 84-85 1.1663540337498581 1102 86-87 1.1976742496175312 1102 88-89 1.0048062639449178 1102 90-91 1.095030356631863 1102 92-93 1.1210985558086044 1102 94-95 1.2968107953546415 1102 96-97 1.0927227422846002 1102 98-99 1.1191673868269447 1102 100-101 0.6280333417172521 1103 1 0.3104104982723186 1103 2 0.3644399082513381 1103 3 0.3523617095615599 1103 4 0.19989634806908896 1103 5 0.18814509167145133 1103 6 0.3173581905344136 1103 7 0.3226808544148625 1103 8 0.25252609509360013 1103 9 0.33453313782724337 1103 10-11 0.3795891596279617 1103 12-13 0.33906650836816965 1103 14-15 0.46868938556448825 1103 16-17 0.4657404656802342 1103 18-19 0.23437410311567675 1103 20-21 0.36386445311443083 1103 22-23 0.4103501049256906 1103 24-25 0.4959089720080314 1103 26-27 0.40193406899836503 1103 28-29 0.7142526435102283 1103 30-31 0.7683511455885252 1103 32-33 0.6047619185570383 1103 34-35 0.5522353968783023 1103 36-37 0.6046596206775945 1103 38-39 0.5286380070871459 1103 40-41 0.700522382237402 1103 42-43 0.7110658001378241 1103 44-45 0.8593569285478182 1103 46-47 0.9661941973559749 1103 48-49 1.1341916721383285 1103 50-51 0.83556139485313 1103 52-53 0.6551998339441667 1103 54-55 0.6387974739364779 1103 56-57 0.673609983393284 1103 58-59 0.6982189464481969 1103 60-61 0.5542813873418275 1103 62-63 0.7590240827784207 1103 64-65 0.6413642972618376 1103 66-67 0.5672836077941596 1103 68-69 0.7089595304691549 1103 70-71 0.5472880580907677 1103 72-73 0.9114668376061168 1103 74-75 0.8100348978441865 1103 76-77 0.5582289602131745 1103 78-79 0.45770734262060486 1103 80-81 0.7602185413204303 1103 82-83 0.8020500243697342 1103 84-85 1.0656999259528952 1103 86-87 0.9416520971668056 1103 88-89 0.9966655290184683 1103 90-91 0.982102279418072 1103 92-93 1.1876939974040432 1103 94-95 1.286096509640359 1103 96-97 1.4272094856999118 1103 98-99 1.2158067263234287 1103 100-101 0.995939179265946 1104 1 0.2909374357354082 1104 2 0.5337672676338236 1104 3 0.4375488390757418 1104 4 0.28097669017378024 1104 5 0.21798021257225741 1104 6 0.28793806235452024 1104 7 0.1378632689312198 1104 8 0.24468801881270963 1104 9 0.4358229570775549 1104 10-11 0.3062250694402522 1104 12-13 0.3197748152707831 1104 14-15 0.5165630187619641 1104 16-17 0.593322170030433 1104 18-19 0.5179341330583469 1104 20-21 0.4699114985849988 1104 22-23 0.4893831293537616 1104 24-25 0.4534265985656205 1104 26-27 0.5482764215878433 1104 28-29 0.48220882562580414 1104 30-31 0.6849467227261314 1104 32-33 0.7943816928690524 1104 34-35 0.8445294134488393 1104 36-37 0.6311981925193422 1104 38-39 0.8941603146168049 1104 40-41 0.8490432947110023 1104 42-43 0.9109030233443605 1104 44-45 1.075867380531399 1104 46-47 1.301412010812193 1104 48-49 1.1256709540976289 1104 50-51 1.1776469428987113 1104 52-53 1.1873019209383102 1104 54-55 1.1261131576233154 1104 56-57 1.4965803469585026 1104 58-59 1.6036999491386617 1104 60-61 1.4142058501601866 1104 62-63 1.19795969632359 1104 64-65 1.3502174348012375 1104 66-67 1.377452083902 1104 68-69 1.2220983336860485 1104 70-71 0.9403918876897741 1104 72-73 0.927708972187709 1104 74-75 1.1780110093320069 1104 76-77 0.9022381787630458 1104 78-79 1.3052157457675015 1104 80-81 1.1930516960185358 1104 82-83 1.0559599484679936 1104 84-85 1.0593975657805252 1104 86-87 1.3060962135057324 1104 88-89 1.444093303272151 1104 90-91 1.4722770381389942 1104 92-93 1.481206561415 1104 94-95 1.5431398414425317 1104 96-97 1.5407677171382552 1104 98-99 1.33390891539484 1104 100-101 1.0847476215424656 1105 1 0.19527318939724125 1105 2 0.218164280566711 1105 3 0.2439841119471673 1105 4 0.13746041479166848 1105 5 0.19544083282237779 1105 6 0.2112386088766698 1105 7 0.17318984386709957 1105 8 0.1358714950730473 1105 9 0.1866318979494821 1105 10-11 0.2588613696242348 1105 12-13 0.27258645656522873 1105 14-15 0.386150738446581 1105 16-17 0.43442373573336823 1105 18-19 0.3443366925303266 1105 20-21 0.22489770847253965 1105 22-23 0.2675160599390054 1105 24-25 0.2556536847860116 1105 26-27 0.47048934499004247 1105 28-29 0.4190347788207376 1105 30-31 0.5614523692181237 1105 32-33 0.7817135094029553 1105 34-35 0.7055686187046746 1105 36-37 0.9059191714479127 1105 38-39 0.7440539240686164 1105 40-41 0.8190248118944368 1105 42-43 1.0311891427660527 1105 44-45 0.971810902058543 1105 46-47 1.0943650769945634 1105 48-49 0.8759009304816345 1105 50-51 1.1619056311777953 1105 52-53 1.251232814517266 1105 54-55 1.2679480218737211 1105 56-57 1.1659856949470822 1105 58-59 1.3830966910128346 1105 60-61 1.3557181545320134 1105 62-63 1.406193359106208 1105 64-65 1.3555361151518497 1105 66-67 1.2262750114039562 1105 68-69 1.42052009428021 1105 70-71 1.1356210847799595 1105 72-73 1.2689884201766226 1105 74-75 1.2529007223147062 1105 76-77 0.9877864289095832 1105 78-79 0.8937075242176569 1105 80-81 1.0156335981053495 1105 82-83 1.0357152502071756 1105 84-85 1.0197260042451184 1105 86-87 1.3635593918725348 1105 88-89 1.499919541078743 1105 90-91 1.8794525554376236 1105 92-93 1.3555119734411996 1105 94-95 1.5208799909991804 1105 96-97 1.518833560689938 1105 98-99 1.597671074919674 1105 100-101 1.1753506933154263 1106 1 0.0579406057216616 1106 2 0.2547336778989724 1106 3 0.06830603782289657 1106 4 -0.030747146077686693 1106 5 0.06528212630469454 1106 6 -0.06732006239220567 1106 7 0.13954957284844483 1106 8 0.17537250102935076 1106 9 0.14375246749079196 1106 10-11 0.2028052147975714 1106 12-13 0.1871310580985508 1106 14-15 0.17166794644310102 1106 16-17 0.16982744008523554 1106 18-19 0.12364626270586143 1106 20-21 -0.199109460439999 1106 22-23 -0.12333364733454033 1106 24-25 0.09886723422928867 1106 26-27 0.06954221944346273 1106 28-29 0.1989698912259641 1106 30-31 0.13050590148382213 1106 32-33 0.17169006398088982 1106 34-35 0.03007113266455974 1106 36-37 0.2726029660565743 1106 38-39 0.2185773514939271 1106 40-41 -0.04557880656948754 1106 42-43 0.28890447388765494 1106 44-45 0.3959105370347942 1106 46-47 0.36262029822427877 1106 48-49 0.2689400355590834 1106 50-51 0.42781879251183597 1106 52-53 0.35909851159347994 1106 54-55 0.24813111709141822 1106 56-57 0.4358606237357989 1106 58-59 0.4502922139177308 1106 60-61 0.5124394444398135 1106 62-63 0.5543308195288681 1106 64-65 0.4162521872431171 1106 66-67 0.3918978665325952 1106 68-69 0.39808559314392866 1106 70-71 0.3554470499953446 1106 72-73 0.27569686412791583 1106 74-75 0.40140556468178445 1106 76-77 0.2351250818341839 1106 78-79 0.026968498201799918 1106 80-81 0.1602970650738662 1106 82-83 0.2804760237447539 1106 84-85 0.4625894238015036 1106 86-87 0.3400159185493017 1106 88-89 0.49796223930053785 1106 90-91 0.3427199461942436 1106 92-93 0.10321552599977935 1106 94-95 -0.030073774727810587 1106 96-97 -0.0073033064605319 1106 98-99 0.3819018255331166 1106 100-101 0.24960963056385665 1107 1 0.19717635999549543 1107 2 0.3314351171361345 1107 3 0.20205138622904073 1107 4 0.1880525667318551 1107 5 0.09340989790381116 1107 6 0.1712216482681015 1107 7 0.18803877270391212 1107 8 0.09429281506179876 1107 9 0.039974411282173605 1107 10-11 0.10786577350502569 1107 12-13 0.19006162088860634 1107 14-15 0.39272717243665056 1107 16-17 0.2772141476718488 1107 18-19 0.2569293846351073 1107 20-21 0.20212893110571883 1107 22-23 0.3451129432172806 1107 24-25 0.3061464800258449 1107 26-27 0.09405899333728485 1107 28-29 0.3155330848589699 1107 30-31 0.444517229551991 1107 32-33 0.3411512064545832 1107 34-35 0.19946657797402167 1107 36-37 0.2853036356983907 1107 38-39 0.3000512204468535 1107 40-41 0.43820807182975585 1107 42-43 0.3641089563113127 1107 44-45 0.6203085867675497 1107 46-47 0.22191808939176383 1107 48-49 0.34290331084427805 1107 50-51 0.2596880176137901 1107 52-53 0.3670267515195249 1107 54-55 0.34654546910621065 1107 56-57 0.3552853981364308 1107 58-59 0.47570763116511117 1107 60-61 0.6212832212759309 1107 62-63 0.28179317270744164 1107 64-65 0.436909252738495 1107 66-67 0.6179125357708379 1107 68-69 0.6717659013574853 1107 70-71 0.5814898751611111 1107 72-73 0.44689428879907567 1107 74-75 0.6441331232343863 1107 76-77 0.27177236783740355 1107 78-79 0.709127963990003 1107 80-81 0.9857608166635039 1107 82-83 0.8479751673352887 1107 84-85 0.9630493039511059 1107 86-87 0.8032442771995107 1107 88-89 0.7413843107321263 1107 90-91 0.881040163558005 1107 92-93 0.7189762756982745 1107 94-95 0.8301109281567634 1107 96-97 0.8625881524388532 1107 98-99 1.1870088415209885 1107 100-101 0.7521765126233717 1108 1 -0.14622648589316256 1108 2 -0.0413082049913136 1108 3 -0.23877049883963153 1108 4 0.0252200259273323 1108 5 0.10169368409464852 1108 6 0.030615989409476185 1108 7 -0.06996486638996657 1108 8 0.06852153684698692 1108 9 -0.08307385969729353 1108 10-11 -0.11130846333369249 1108 12-13 -0.17577666715897067 1108 14-15 -0.14431650345401437 1108 16-17 0.028630666091657986 1108 18-19 -0.22496420681685692 1108 20-21 -0.03017107092351523 1108 22-23 -0.19664870289775394 1108 24-25 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86-87 -0.26499469748742754 2316 88-89 -0.3197536392755076 2316 90-91 -0.5472884597650456 2316 92-93 -0.10920485372474076 2316 94-95 0.03782670280222078 2316 96-97 0.013913436983969518 2316 98-99 0.46969300004236203 2316 100-101 0.3760094527356621 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 15.0 5 29.0 6 73.0 7 188.0 8 415.0 9 806.0 10 1263.0 11 2054.0 12 2927.0 13 3790.0 14 5081.0 15 6643.0 16 8891.0 17 11327.0 18 9771.0 19 6271.0 20 5576.0 21 5633.0 22 5876.0 23 6492.0 24 7426.0 25 8734.0 26 10213.0 27 12572.0 28 15154.0 29 18277.0 30 22116.0 31 26937.0 32 32741.0 33 40580.0 34 52548.0 35 70463.0 36 92178.0 37 106992.0 38 52404.0 39 1972.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.95590792807607 19.45494668056324 15.327695237163756 21.261450154196936 2 16.22050757759837 21.049784294038627 39.03536721972361 23.694340908639393 3 19.325666188870922 25.65778414838767 30.621613278850358 24.39493638389105 4 13.047783348333597 17.508438602576582 36.39394712674942 33.049830922340405 5 13.018031784841074 38.45048899755501 33.20125305623472 15.330226161369193 6 31.037507219979403 36.929468823441326 16.58165682100869 15.451367135570585 7 27.98485397193584 34.630953090404 19.844475107307286 17.53971783035287 8 27.44418807206271 33.55454364867136 19.105939519887535 19.89532875937839 9 27.417779971364524 16.381814486738904 20.231712031636544 35.968693510260024 10-11 25.354275603040183 27.00356338454298 25.929047084961965 21.71311392745487 12-13 26.984462909934386 24.81006488049215 27.088675099398866 21.11679711017459 14-15 23.229787279559062 25.709895832537473 26.715049959735772 24.345266928167693 16-17 21.243537963841632 29.047110402063574 27.579122217485054 22.130229416609744 18-19 21.264255846167128 26.133495918933352 31.77394794968709 20.82830028521243 20-21 22.153417595019782 25.007124526018465 32.19155004473362 20.647907834228132 22-23 21.746508228897905 25.104231688114037 32.255094689054545 20.894165393933513 24-25 21.74559140261433 25.37752232280995 32.26991671397236 20.606969560603364 26-27 21.135990134979338 25.782222334278426 32.085460172805945 20.99632735793629 28-29 20.94315159599868 26.15590430502106 31.97207966890448 20.928864430075784 30-31 21.126536736903308 25.6716335806476 31.995146925456563 21.20668275699253 32-33 20.871978282678175 25.8464705149765 31.782823725547576 21.49872747679775 34-35 21.55581430529515 25.789657854404375 31.569964044991895 21.08456379530858 36-37 21.936898563237055 25.42233801557862 31.532610813350704 21.108152607833624 38-39 21.564574912705627 25.760893649859796 31.413672170478073 21.2608592669565 40-41 21.357921501633474 26.0024693093574 31.32057625367875 21.319032935330377 42-43 21.50845714582598 26.061440946674676 30.82606350385152 21.604038403647827 44-45 21.445670087890274 26.34752225527223 30.662038476731578 21.544769180105913 46-47 22.166864802256605 26.82906144851887 29.692146190448543 21.31192755877598 48-49 22.443199399770634 26.865230323501926 28.845696891811258 21.84587338491618 50-51 22.12804191813037 27.31073464457377 28.88755437078872 21.673669066507138 52-53 22.589320573051367 27.313915313637203 28.560961391386197 21.53580272192523 54-55 21.822196415201013 27.367021682991304 28.80483779777822 22.00594410402946 56-57 22.17691004577829 27.45543474855563 28.420209689164583 21.947445516501503 58-59 22.33490980494029 26.93099944224995 28.441356020262372 22.29273473254739 60-61 21.935496687426127 27.073099730211087 29.232786344142465 21.758617238220328 62-63 21.810227655606433 27.192991168670822 29.330800855398927 21.665980320323825 64-65 21.565250611246945 27.30187958435208 29.01818459657702 22.11468520782396 66-67 21.879639102202166 27.220828714127776 28.61251162282528 22.287020560844777 68-69 22.084686322899078 27.06898527692672 28.766149920157698 22.080178480016503 70-71 21.991726608260862 27.195604435907196 28.497586290681205 22.315082665150744 72-73 21.98538209635347 27.16070983782165 28.404668071509455 22.449239994315427 74-75 21.84461605582642 27.75772079301879 28.001231689883106 22.396431461271682 76-77 21.95778090353359 27.36891244836072 28.31280241496471 22.360504233140972 78-79 22.04779278332792 27.53590189299154 27.965005444022083 22.45129987965846 80-81 22.033358292202138 28.0099631729344 27.627863266148132 22.32881526871533 82-83 22.168780562347187 27.859031173594133 27.465770171149146 22.506418092909534 84-85 21.647464936053233 27.865418012978072 27.545665074651044 22.941451976317655 86-87 21.80505299316651 27.90560859626175 27.21889783993252 23.07044057063922 88-89 21.542119554836333 27.814783064465605 27.034233837756393 23.60886354294167 90-91 21.635245403718592 27.83908869804947 27.312391258335428 23.213274639896508 92-93 21.664389799635703 27.423411694233568 27.561016980232516 23.351181525898216 94-95 21.274123220865903 27.449912251414027 27.58972932789244 23.686235199827635 96-97 21.412816181397414 27.444167104069873 27.536537493286144 23.606479221246566 98-99 21.197451801052225 27.67881018851721 27.000273523393894 24.12346448703667 100-101 21.017674604817902 27.550088743584904 27.037110328561376 24.395126323035818 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 472.0 1 365.0 2 328.0 3 400.0 4 357.0 5 315.5 6 484.0 7 904.0 8 1392.0 9 1648.0 10 1501.5 11 1279.0 12 1245.5 13 1314.0 14 1419.0 15 1466.5 16 1563.0 17 1656.0 18 1739.5 19 1943.0 20 2127.0 21 2286.5 22 2529.0 23 2938.5 24 3352.5 25 3829.0 26 4534.0 27 5474.0 28 6381.0 29 7453.0 30 8736.5 31 10162.5 32 11890.0 33 12885.0 34 13081.0 35 13746.5 36 15328.0 37 16776.0 38 17270.0 39 17379.0 40 17600.5 41 18394.5 42 19085.5 43 19625.0 44 20310.0 45 21334.0 46 21728.0 47 22111.5 48 24527.5 49 24308.5 50 21900.0 51 20742.0 52 20442.0 53 20682.0 54 20762.5 55 19744.0 56 17717.5 57 16322.0 58 15985.5 59 14573.5 60 11733.5 61 9801.5 62 8635.0 63 6688.0 64 4564.5 65 3392.0 66 2593.5 67 2141.5 68 1685.0 69 1181.5 70 908.5 71 691.5 72 587.5 73 571.0 74 453.0 75 338.0 76 248.0 77 166.5 78 116.5 79 88.0 80 73.0 81 59.0 82 47.0 83 30.5 84 11.5 85 6.0 86 3.5 87 2.0 88 3.0 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011613088562024589 2 0.010849069577680866 3 0.006112151874749784 4 1.528037968687446E-4 5 0.005195329093537316 6 0.0 7 7.64018984343723E-4 8 6.112151874749784E-4 9 1.528037968687446E-4 10-11 0.0 12-13 0.0 14-15 0.0010696265780812122 16-17 0.005348132890406061 18-19 0.0014516360702530736 20-21 6.876170859093507E-4 22-23 3.820094921718615E-4 24-25 3.820094921718615E-4 26-27 7.64018984343723E-5 28-29 7.64018984343723E-5 30-31 5.348132890406061E-4 32-33 0.003896496820152987 34-35 7.64018984343723E-4 36-37 0.001757243663990563 38-39 0.005959348077881039 40-41 0.0 42-43 0.002597664546768658 44-45 0.005882946179446667 46-47 0.0 48-49 0.0026740664452030304 50-51 0.0023684588514655412 52-53 0.0011460284765155845 54-55 6.112151874749784E-4 56-57 0.012911920835408918 58-59 0.0029032721405061472 60-61 0.005118927195102944 62-63 3.820094921718615E-4 64-65 0.005195329093537316 66-67 0.0016044398671218184 68-69 0.0029032721405061472 70-71 0.0071817784528309956 72-73 0.0047369177029310825 74-75 0.007410984148134112 76-77 0.0022156550545967967 78-79 0.007105376554396623 80-81 0.00366729112484987 82-83 0.005195329093537316 84-85 0.003438085429546753 86-87 0.001528037968687446 88-89 0.003514487327981126 90-91 0.01123107906985273 92-93 0.00397289871858736 94-95 0.0011460284765155845 96-97 5.348132890406061E-4 98-99 0.0016808417655561905 100-101 0.003132477835809264 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 654434.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.52934505286191 #Duplication Level Percentage of deduplicated Percentage of total 1 81.81932103909256 48.70650594127026 2 10.609078714120306 12.631030149316805 3 3.187840054678701 5.693100917649079 4 1.3456920404446695 3.2043266324208215 5 0.7418605561664942 2.2081236514571643 6 0.4535057201292663 1.6198139098213036 7 0.31163549557353176 1.2986019862701477 8 0.2338779779401824 1.1138082279253396 9 0.17696688578530406 0.9481250526159394 >10 1.0027250819896125 11.030312597728456 >50 0.07224620955148041 2.8671278520683887 >100 0.04010815355880823 4.723186525368531 >500 0.0033423461298833883 1.3179312757562567 >1k 0.00154262129071541 1.8367777540762786 >5k 2.5710354845256836E-4 0.8012275262552554 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5235 0.7999278766078779 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4558 0.6964797061277379 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2850 0.43549082107592213 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1233 0.18840708153916208 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1227 0.1874902587579496 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1092 0.1668617461806691 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1041 0.15906875254036312 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 949 0.14501080322843862 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 867 0.13248089188520157 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 854 0.1304944425259079 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 664 0.10146172112084641 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 662 0.10115611352710893 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 7.64018984343723E-5 0.0 20-21 0.0 0.0 0.0 2.292056953031169E-4 0.0 22-23 0.0 0.0 0.0 3.056075937374892E-4 0.0 24-25 0.0 0.0 0.0 7.64018984343723E-4 0.0 26-27 0.0 0.0 0.0 0.0016044398671218182 0.0 28-29 0.0 0.0 0.0 0.002826870242071775 0.0 30-31 0.0 0.0 0.0 0.0061121518747497845 0.0 32-33 0.0 0.0 0.0 0.010085050593337143 0.0 34-35 0.0 0.0 0.0 0.014134351210358876 0.0 36-37 0.0 0.0 0.0 0.01932968030389619 0.0 38-39 0.0 0.0 0.0 0.028039496725414635 0.0 40-41 0.0 0.0 0.0 0.04866800930269515 0.0 42-43 0.0 0.0 0.0 0.08129161993417212 0.0 44-45 0.0 0.0 0.0 0.11429724005782096 0.0 46-47 0.0 0.0 0.0 0.18000287271138113 0.0 48-49 0.0 0.0 0.0 0.24861177750544744 0.0 50-51 0.0 0.0 0.0 0.2998774513549113 0.0 52-53 0.0 0.0 0.0 0.3586305112509436 0.0 54-55 0.0 0.0 0.0 0.41616114077202593 0.0 56-57 0.0 0.0 0.0 0.5312223998141905 0.0 58-59 0.0 0.0 0.0 0.6795184846753073 0.0 60-61 0.0 0.0 0.0 0.8391984524031453 0.0 62-63 0.0 0.0 0.0 0.9997952429121959 0.0 64-65 0.0 0.0 0.0 1.1910291946934297 0.0 66-67 0.0 0.0 0.0 1.4429262538315553 0.0 68-69 0.0 0.0 0.0 1.7369971609054542 0.0 70-71 0.0 0.0 0.0 1.9848449194265578 0.0 72-73 0.0 0.0 0.0 2.196707383785072 0.0 74-75 0.0 0.0 0.0 2.410861905096618 0.0 76-77 0.0 0.0 0.0 2.676664109749799 0.0 78-79 0.0 0.0 0.0 2.954461412457177 0.0 80-81 0.0 0.0 0.0 3.3041529015912987 0.0 82-83 0.0 0.0 0.0 3.629395783226421 0.0 84-85 0.0 0.0 0.0 4.082535442840684 0.0 86-87 0.0 0.0 0.0 4.60512442813179 0.0 88-89 0.0 0.0 0.0 5.280899219783813 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTAAG 330 0.0 37.422043 2 TAGTACG 65 1.3280183E-4 36.53632 4 GGTATCA 4115 0.0 34.16828 1 TAAGGTG 400 0.0 33.24805 5 GTAAGGT 390 0.0 32.882687 4 GTATCAA 6115 0.0 31.382404 1 GGTAAGG 415 0.0 29.75729 3 CTATATG 90 8.9424866E-4 26.389357 1 AAGGTAA 490 0.0 25.204529 1 ATCAACG 7615 0.0 25.13641 3 TCAACGC 7685 0.0 24.845646 4 TATCAAC 7720 0.0 24.794529 2 CAACGCA 7720 0.0 24.733004 5 AACGCAG 7880 0.0 24.22896 6 AAGGTGT 610 0.0 24.136084 6 AGGTGTG 615 0.0 23.939856 7 GAGTACT 4960 0.0 23.223698 12-13 GTGTAAC 230 3.61797E-9 22.717793 1 AGTACGG 105 0.0021959678 22.617718 5 GTACTTT 5230 0.0 21.888533 14-15 >>END_MODULE