FastQCFastQC Report
Thu 26 May 2016
SRR936738_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936738_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753084
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52950.7031088165463614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40970.5440296168820477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24320.3229387425572712No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10610.1408873379330858No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10150.13477912158537428No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9610.12760860674240854No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA7860.10437082715872334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37400.037.1054571
GTATCAA59300.035.183291
TCGCGAT300.00395767231.65630752-53
ATCAACG72850.028.441643
TCAACGC73250.028.2233264
CAACGCA73700.028.1173535
TATCAAC74150.027.8807642
AACGCAG75050.027.6749066
TACGGGT700.00836784127.1574214
GTACATG46100.025.3606741
ACGCAGA83200.025.074887
GAGTACT45300.024.86267512-13
CGCAGAG84200.024.7770798
TCTACAC3650.024.737653
TAAGGTG3500.024.4433025
TACATGG48050.024.133652
GCAGAGT86750.023.7748589
GTAAGGT4000.023.7627434
GGTAAGG3600.023.7611663
ACATGGG47250.023.5348683
Produced by FastQC (version 0.11.5)