##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936738_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 753084 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.357188573917384 31.0 31.0 34.0 30.0 34.0 2 31.39233471963287 31.0 31.0 34.0 28.0 34.0 3 31.39318588630219 31.0 31.0 34.0 28.0 34.0 4 35.07571532524924 37.0 35.0 37.0 32.0 37.0 5 35.037485858151285 37.0 35.0 37.0 32.0 37.0 6 35.1301289099224 37.0 35.0 37.0 32.0 37.0 7 34.98368176723978 37.0 35.0 37.0 32.0 37.0 8 34.96059403731854 37.0 35.0 37.0 32.0 37.0 9 36.49198894147266 39.0 37.0 39.0 32.0 39.0 10-11 36.34426571272262 39.0 36.0 39.0 32.0 39.0 12-13 36.24502379548629 39.0 35.0 39.0 32.0 39.0 14-15 37.27077789463061 39.5 36.5 41.0 32.0 41.0 16-17 37.237576419098005 39.0 36.5 41.0 32.0 41.0 18-19 37.23758173059048 39.5 36.5 41.0 31.0 41.0 20-21 37.167159971530396 39.0 36.0 41.0 31.0 41.0 22-23 37.052067896808325 39.0 36.0 41.0 31.0 41.0 24-25 36.883584699714774 39.0 36.0 41.0 30.5 41.0 26-27 36.68755942232208 39.0 36.0 41.0 30.0 41.0 28-29 36.72682994194539 39.0 36.0 41.0 30.0 41.0 30-31 36.58442218929097 39.0 35.5 40.5 30.0 41.0 32-33 36.34092611182816 39.0 35.0 40.0 30.0 41.0 34-35 36.11893825921146 38.5 35.0 40.0 30.0 41.0 36-37 35.831664196822665 38.0 35.0 40.0 28.5 41.0 38-39 35.61622674230232 38.0 35.0 40.0 27.5 41.0 40-41 35.418021761184676 38.0 35.0 40.0 27.0 41.0 42-43 35.06945573136596 38.0 34.0 40.0 26.0 41.0 44-45 34.70125709748182 38.0 34.0 40.0 24.5 41.0 46-47 34.21049179108838 38.0 33.0 40.0 23.5 41.0 48-49 33.86255384525498 38.0 33.0 40.0 21.5 41.0 50-51 33.11092056131852 37.0 32.0 39.5 19.5 40.5 52-53 33.25813853434676 37.5 33.0 39.5 20.0 40.5 54-55 33.600095739651884 38.0 33.0 40.0 19.5 41.0 56-57 33.503546749101034 38.0 33.0 40.0 18.5 41.0 58-59 33.24610202845898 37.0 33.0 40.0 17.0 41.0 60-61 32.93231166775553 37.0 32.5 40.0 13.5 41.0 62-63 32.60431306467804 36.0 32.0 40.0 12.5 41.0 64-65 32.27784948292621 36.0 31.5 39.0 8.5 41.0 66-67 31.907084070302915 35.5 31.0 39.0 7.5 41.0 68-69 31.522737171417795 35.0 31.0 39.0 6.5 40.0 70-71 31.09153958920917 35.0 30.5 38.0 2.0 40.0 72-73 30.694831519458653 35.0 30.0 37.0 2.0 39.5 74-75 30.282902040144258 35.0 30.0 37.0 2.0 39.0 76-77 29.8730951660107 34.5 29.0 36.0 2.0 39.0 78-79 29.418495147951624 34.0 29.0 36.0 2.0 38.5 80-81 29.026700872678212 34.0 29.0 35.0 2.0 37.0 82-83 28.630537230906512 34.0 28.5 35.0 2.0 37.0 84-85 28.245706986206052 34.0 27.5 35.0 2.0 36.0 86-87 27.926128426576582 34.0 27.0 35.0 2.0 36.0 88-89 27.52343032118595 34.0 26.0 35.0 2.0 36.0 90-91 27.158506222413436 33.0 25.5 35.0 2.0 35.0 92-93 26.773492067285986 33.0 25.0 35.0 2.0 35.0 94-95 26.331911845159368 33.0 24.5 35.0 2.0 35.0 96-97 25.735734659081857 33.0 22.0 35.0 2.0 35.0 98-99 25.066298713025375 32.5 18.0 35.0 2.0 35.0 100-101 23.145088064545256 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4180096189819551 1101 2 0.5298034073510465 1101 3 0.44166873360169845 1101 4 0.483897646478745 1101 5 0.5844749303353751 1101 6 0.5429618068805198 1101 7 0.5119033077268043 1101 8 0.40623927617891553 1101 9 0.7437609856037781 1101 10-11 0.6463181391480006 1101 12-13 0.782979013884308 1101 14-15 0.8208704378089351 1101 16-17 0.8364814972642662 1101 18-19 0.8199952482838739 1101 20-21 0.7661136155803376 1101 22-23 0.619440022216871 1101 24-25 0.5967868294293126 1101 26-27 0.6270507248971597 1101 28-29 0.8916295596045813 1101 30-31 0.7229905542506003 1101 32-33 0.7248167304112414 1101 34-35 0.7438813041529428 1101 36-37 0.711213386244367 1101 38-39 0.6561103651015614 1101 40-41 0.658105733815205 1101 42-43 0.42116717642851853 1101 44-45 0.6378317188560487 1101 46-47 0.6306946814908443 1101 48-49 0.6604554841022008 1101 50-51 0.9192134049384961 1101 52-53 0.9273378680609881 1101 54-55 1.0354393839476757 1101 56-57 1.0242221613629425 1101 58-59 1.0541632710910491 1101 60-61 0.8144801523021741 1101 62-63 0.8615900732494381 1101 64-65 0.8394134870307788 1101 66-67 0.7848158360639879 1101 68-69 0.8675230183721254 1101 70-71 0.6837051509257925 1101 72-73 0.7654526999110338 1101 74-75 0.7321515371486775 1101 76-77 0.8109452452437615 1101 78-79 0.9191528071248349 1101 80-81 0.8107451488978548 1101 82-83 0.7173728272745201 1101 84-85 0.8293567823227121 1101 86-87 0.746111097885116 1101 88-89 0.6509026978052539 1101 90-91 0.6857193451839692 1101 92-93 0.6225834221863913 1101 94-95 0.4676500616220878 1101 96-97 0.3880657612073968 1101 98-99 0.2612632968690427 1101 100-101 0.5507063775296963 1102 1 0.1657723915169349 1102 2 0.2275799258604252 1102 3 0.1929072739475366 1102 4 0.17957860521838143 1102 5 0.20001405306167896 1102 6 0.1970976619923377 1102 7 -0.004245406884329839 1102 8 0.07856750760501541 1102 9 0.2570917754642963 1102 10-11 0.34908598927760437 1102 12-13 0.4460181927166218 1102 14-15 0.4057420111338885 1102 16-17 0.16264471528102575 1102 18-19 0.2547250136485104 1102 20-21 -0.01165816197052294 1102 22-23 -0.3827249687500611 1102 24-25 -0.09672703436772423 1102 26-27 0.04385531885791494 1102 28-29 0.03741122864639834 1102 30-31 -0.007764380273677318 1102 32-33 -0.2757232900113493 1102 34-35 -0.09628387196384125 1102 36-37 -0.20644401918894317 1102 38-39 -0.16447591473281165 1102 40-41 -0.16447862158275228 1102 42-43 0.1733391806476945 1102 44-45 0.22214643995302907 1102 46-47 0.4550905599427679 1102 48-49 0.48588371328406055 1102 50-51 0.4809678962252306 1102 52-53 0.42050766170304854 1102 54-55 0.18155137717006653 1102 56-57 0.3425649018052894 1102 58-59 0.06028093102124643 1102 60-61 -0.1233529771792945 1102 62-63 0.14685410816981914 1102 64-65 0.28491630916348143 1102 66-67 0.2002114676494351 1102 68-69 0.2258504138185593 1102 70-71 0.3798461790124641 1102 72-73 0.015774608188916517 1102 74-75 0.04010624837100352 1102 76-77 0.2166527701828862 1102 78-79 0.09988782401275031 1102 80-81 0.2345356434162973 1102 82-83 0.07769970093498912 1102 84-85 -0.19261012634791186 1102 86-87 -0.12170101989263671 1102 88-89 -0.19055712467530483 1102 90-91 -0.39281505341562806 1102 92-93 -0.5299758538215542 1102 94-95 -0.43208848433435065 1102 96-97 -0.7570138812850544 1102 98-99 -0.6904083819352245 1102 100-101 -0.5808797676502344 1103 1 0.17784342104606665 1103 2 0.3902722229680542 1103 3 0.30361055159841754 1103 4 0.25117917763768105 1103 5 0.23348200583708234 1103 6 0.028966693557961776 1103 7 0.11486871921304243 1103 8 0.02281166645137489 1103 9 0.3192770136512735 1103 10-11 0.3970777057098829 1103 12-13 0.23666374683501346 1103 14-15 0.4709751068765158 1103 16-17 0.5803302011494296 1103 18-19 0.38894082673159147 1103 20-21 0.24029232926430666 1103 22-23 0.28688348819464693 1103 24-25 0.4352393497586533 1103 26-27 0.4894204453130371 1103 28-29 0.424370457531559 1103 30-31 0.20923722965559932 1103 32-33 0.4345565212003635 1103 34-35 0.45686615418031096 1103 36-37 0.5396515583215873 1103 38-39 0.4415936562279157 1103 40-41 0.40101591566577355 1103 42-43 0.5388498859893573 1103 44-45 0.4854403965262222 1103 46-47 0.6659035742257302 1103 48-49 0.6011536172387082 1103 50-51 0.2763623976975538 1103 52-53 0.49428232664512706 1103 54-55 0.6777209151066046 1103 56-57 0.653551293086899 1103 58-59 0.568050215772324 1103 60-61 0.713724244385503 1103 62-63 0.5733890450110621 1103 64-65 0.6947275896764573 1103 66-67 0.6379625890958955 1103 68-69 0.5110882567885362 1103 70-71 0.8919258085911324 1103 72-73 0.7941547716586577 1103 74-75 0.6612207459905086 1103 76-77 0.4810165729183886 1103 78-79 0.6703824300616823 1103 80-81 1.0786391054522397 1103 82-83 1.2393771921973382 1103 84-85 0.6867586988165222 1103 86-87 0.5303082956810208 1103 88-89 0.43811509647668956 1103 90-91 0.6277218648770209 1103 92-93 0.7191159809474144 1103 94-95 0.6890672935229283 1103 96-97 0.8674047553662909 1103 98-99 0.8792864195068333 1103 100-101 0.6651814096621465 1104 1 0.32092436720082773 1104 2 0.16773923830146842 1104 3 0.32590224302834514 1104 4 0.2615417118109846 1104 5 0.34283241591672464 1104 6 0.23427039021789398 1104 7 0.39637769341962326 1104 8 0.3967181207482966 1104 9 0.6416667676034891 1104 10-11 0.5870298630853767 1104 12-13 0.5687278143495789 1104 14-15 0.5430017323371175 1104 16-17 0.29739605161893934 1104 18-19 0.39339732263079696 1104 20-21 0.3469460199810612 1104 22-23 0.563193960083396 1104 24-25 0.36314824076067254 1104 26-27 0.2739622258907204 1104 28-29 0.31551362881409517 1104 30-31 0.3148446229754569 1104 32-33 0.23511310030413313 1104 34-35 0.23906305183869136 1104 36-37 0.3739752240769647 1104 38-39 0.41604420894395133 1104 40-41 0.3811680611549022 1104 42-43 0.20122121607375476 1104 44-45 0.134247896823382 1104 46-47 0.2191265624582357 1104 48-49 0.13961314416132353 1104 50-51 0.07163816139659218 1104 52-53 0.35751452681153495 1104 54-55 0.6074451514075747 1104 56-57 0.5440312051277871 1104 58-59 0.6272663099123506 1104 60-61 0.5494522843236922 1104 62-63 0.5883094063566006 1104 64-65 0.5454838597346985 1104 66-67 0.5673855060101936 1104 68-69 0.6902223350008256 1104 70-71 0.6100248814545566 1104 72-73 0.33338780387071054 1104 74-75 0.2030949288028232 1104 76-77 0.32736626981604644 1104 78-79 0.2684883375857474 1104 80-81 0.3404000706251864 1104 82-83 0.3389605750571967 1104 84-85 0.4122436625631032 1104 86-87 0.210992356063759 1104 88-89 0.24787647767245957 1104 90-91 0.04983823240347007 1104 92-93 -0.027457773955710252 1104 94-95 0.2881986973027928 1104 96-97 0.42593470900946073 1104 98-99 0.5686320750399396 1104 100-101 0.7442729941147768 1105 1 0.17648667723122102 1105 2 0.15699061734008168 1105 3 0.24788355564795594 1105 4 0.318540473490593 1105 5 0.3959180851726032 1105 6 0.3809190449516535 1105 7 0.339802212163292 1105 8 0.055391992188127404 1105 9 0.4424502866848812 1105 10-11 0.341461924325948 1105 12-13 0.3069491359751524 1105 14-15 0.3879122355454925 1105 16-17 0.3266316702162655 1105 18-19 0.25971725958903136 1105 20-21 0.021330799897747 1105 22-23 0.13013080574364722 1105 24-25 0.14588380307070992 1105 26-27 -0.05120945034479263 1105 28-29 0.24008865977210547 1105 30-31 0.047401191702235224 1105 32-33 0.08000559157959941 1105 34-35 -0.04913462912494282 1105 36-37 -0.04079906388881227 1105 38-39 -0.16817733783079092 1105 40-41 0.022818404507376044 1105 42-43 0.19499809325488826 1105 44-45 0.26586109056423624 1105 46-47 -0.0033590842827351253 1105 48-49 -0.2084854711713291 1105 50-51 -0.11758619974266082 1105 52-53 0.27705071953921134 1105 54-55 0.38026739614470983 1105 56-57 0.4299121012214471 1105 58-59 0.2889504256390225 1105 60-61 0.4575036183324741 1105 62-63 0.20212230740354187 1105 64-65 0.16694057480843227 1105 66-67 -0.30819256446149623 1105 68-69 -0.06501576023726585 1105 70-71 0.1158022089340136 1105 72-73 0.08224742336862789 1105 74-75 0.04088621553027849 1105 76-77 0.04952770176471333 1105 78-79 0.13126667823643245 1105 80-81 0.03148191234532405 1105 82-83 -0.08279108541786684 1105 84-85 -0.33408978334481176 1105 86-87 -0.16812731071548015 1105 88-89 -0.1492371100794294 1105 90-91 0.28010503415963584 1105 92-93 0.18843775683343367 1105 94-95 0.1882910084589362 1105 96-97 0.38083733199719205 1105 98-99 0.17252265254753496 1105 100-101 0.4449754594980959 1106 1 -0.003234393255020507 1106 2 0.18828153886846977 1106 3 0.21746795346454562 1106 4 0.23040265606518062 1106 5 0.5471584699222234 1106 6 0.3716576916269787 1106 7 0.5142713665796776 1106 8 0.5314624086279309 1106 9 0.36801044918435366 1106 10-11 0.3926841605086011 1106 12-13 0.506594540897602 1106 14-15 0.44332958278342005 1106 16-17 0.2957511855099213 1106 18-19 0.5161767533836183 1106 20-21 0.2841918516770079 1106 22-23 0.3296931460711505 1106 24-25 0.20845447777858084 1106 26-27 0.39619879468878594 1106 28-29 -0.009726364449250013 1106 30-31 0.07964561101789513 1106 32-33 0.2748862996529198 1106 34-35 0.1547673127717175 1106 36-37 0.13073770091971681 1106 38-39 0.19885788434955742 1106 40-41 0.21675050094606974 1106 42-43 0.19317765238431406 1106 44-45 0.1359752869317319 1106 46-47 0.27886439437873634 1106 48-49 0.13034687795125421 1106 50-51 0.3383658321988108 1106 52-53 0.14943278875505683 1106 54-55 0.3277887207108918 1106 56-57 0.19367360113070475 1106 58-59 0.09081484188337896 1106 60-61 0.13585098190411315 1106 62-63 0.2732221105613917 1106 64-65 0.2822014255574068 1106 66-67 0.2571143557266886 1106 68-69 0.46544660940715943 1106 70-71 0.7443408304805494 1106 72-73 0.6887529866024273 1106 74-75 0.43548980894653866 1106 76-77 0.25666846484215 1106 78-79 0.2660749316462656 1106 80-81 0.293337812723653 1106 82-83 0.5581869827369808 1106 84-85 0.667148507339391 1106 86-87 0.5732856250246314 1106 88-89 0.817392561431511 1106 90-91 0.9855935796217956 1106 92-93 0.6964545372776563 1106 94-95 0.5096560599031399 1106 96-97 0.5566201634528447 1106 98-99 0.5212599311001149 1106 100-101 1.0799149410074982 1107 1 0.1885607955814912 1107 2 0.3852875335031065 1107 3 0.4375251398502087 1107 4 0.5306256608895907 1107 5 0.31644231532026623 1107 6 0.3616611139782435 1107 7 0.6297205225836962 1107 8 0.35511747977972163 1107 9 0.5891660999278869 1107 10-11 0.7088735379718756 1107 12-13 0.7231852166463781 1107 14-15 0.6005010646615219 1107 16-17 0.6584928200690356 1107 18-19 1.0315055751011926 1107 20-21 1.0912761187970403 1107 22-23 0.6671866601635159 1107 24-25 0.744060730745538 1107 26-27 0.762958932024425 1107 28-29 0.7212823459867153 1107 30-31 0.4860277441363223 1107 32-33 0.6102960434181739 1107 34-35 0.42154235389513417 1107 36-37 0.44778542616011663 1107 38-39 0.7218538768866196 1107 40-41 0.7182712392907504 1107 42-43 0.7280635556090473 1107 44-45 0.488723103802819 1107 46-47 0.7819334706750922 1107 48-49 0.8740376077952661 1107 50-51 0.4012046173745105 1107 52-53 0.4560892619668806 1107 54-55 0.7751590301576812 1107 56-57 0.9729264245705025 1107 58-59 0.7449365671900168 1107 60-61 0.6959242864602544 1107 62-63 1.0290732272414829 1107 64-65 0.6342758120181493 1107 66-67 0.8489879274470304 1107 68-69 0.9829420002727929 1107 70-71 0.8175046286280647 1107 72-73 0.7440901986005244 1107 74-75 0.4347694592043183 1107 76-77 0.5604794185741682 1107 78-79 0.638239072028906 1107 80-81 0.3962070678654612 1107 82-83 0.5276038582406777 1107 84-85 0.6963102520761453 1107 86-87 0.7769569247453241 1107 88-89 0.6707724093024758 1107 90-91 0.2891340572053913 1107 92-93 0.34395235690870507 1107 94-95 0.6220471794535989 1107 96-97 0.7577605792133255 1107 98-99 0.5445788390388664 1107 100-101 1.1165368595113776 1108 1 -0.05779903705449385 1108 2 0.04151303449407706 1108 3 -0.3017349350231662 1108 4 -0.23516601495273903 1108 5 0.06233969475211154 1108 6 -0.07860754931178349 1108 7 0.16255910439387122 1108 8 0.07555350708648234 1108 9 0.15185713714436133 1108 10-11 -0.02636319402548537 1108 12-13 -0.07165292473217022 1108 14-15 0.2500342071411481 1108 16-17 0.2839805980915031 1108 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-1.675149383031215 2316 68-69 -1.5470178083683486 2316 70-71 -1.148167751810142 2316 72-73 -1.1469469784064152 2316 74-75 -1.3342801951200016 2316 76-77 -1.7842664610617085 2316 78-79 -1.3109930930555969 2316 80-81 -1.3138415216877881 2316 82-83 -1.2875871256977796 2316 84-85 -1.235386933029055 2316 86-87 -1.0401873892271745 2316 88-89 -1.3639751199787291 2316 90-91 -1.4838222571870112 2316 92-93 -1.6325214512225479 2316 94-95 -1.847329404580833 2316 96-97 -2.7152114817935598 2316 98-99 -2.8506724319378733 2316 100-101 -3.443247786789666 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1837.0 3 642.0 4 609.0 5 710.0 6 874.0 7 981.0 8 1415.0 9 1770.0 10 2205.0 11 2813.0 12 3755.0 13 4834.0 14 5425.0 15 6746.0 16 8661.0 17 10866.0 18 9080.0 19 5826.0 20 5819.0 21 5873.0 22 6423.0 23 7197.0 24 8260.0 25 9367.0 26 10833.0 27 12642.0 28 14840.0 29 18022.0 30 21680.0 31 26426.0 32 32535.0 33 41535.0 34 54698.0 35 73783.0 36 99131.0 37 123826.0 38 95201.0 39 15941.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.32787233590446 18.249984063237076 14.662220309810673 21.75992329104779 2 15.805024706555704 20.039732337900162 40.276931208078196 23.878311747465936 3 19.1463000047807 25.52932958668204 29.945765628900915 25.378604779636348 4 12.510942408724704 16.524863676516496 37.191703030665316 33.77249088409349 5 13.23287154388594 37.72073163603127 33.68445804836833 15.361938771714467 6 31.050767100643924 36.04363158971742 17.553091124593728 15.352510185044926 7 28.060140828045526 33.92585075308867 20.500429710174767 17.51357870869104 8 27.288980985209243 32.982791713987794 19.68505715063726 20.043170150165707 9 27.454477839905433 16.439376834499885 20.326457970860503 35.77968735473417 10-11 25.024346497492278 26.67678612947155 26.47098681369781 21.827880559338357 12-13 26.748881530219442 24.424257923947376 27.223969629435267 21.602890916397914 14-15 23.310165570038542 25.290737056290624 26.663669423068235 24.735427950602592 16-17 21.564465742944577 28.73683749149949 27.294672836620197 22.404023928935736 18-19 21.56376228496741 26.203325757686446 30.992321189717487 21.240590767628653 20-21 22.61009025043 25.176084632191316 31.039440566870987 21.1743845505077 22-23 22.030947236170896 25.26100358718071 31.205616773290444 21.50243240335795 24-25 21.751018362055515 25.620694577699282 31.27651310176317 21.351773958482035 26-27 21.250093784588252 25.919603535197606 31.19092815868153 21.63937452153261 28-29 21.171199583568757 26.12973240707482 31.19954373158776 21.499524277768668 30-31 21.288423155017934 25.755050602300837 31.071446119604957 21.88508012307627 32-33 20.937633203510398 26.081131145951876 30.980497346220904 22.000738304316823 34-35 21.80646120811857 26.050990387928774 30.636128294625887 21.506420109326772 36-37 21.946801708517558 25.67052276042576 30.617335756366938 21.765339774689746 38-39 21.407627748857962 26.041312546478274 30.55767289918198 21.99338680548178 40-41 21.59743768329176 26.07398928517127 30.608636531736487 21.719936499800482 42-43 21.567178498418826 26.19743333871125 30.02843657276556 22.206951590104364 44-45 21.50133055006693 26.341505194722416 30.125140756793506 22.03202349841715 46-47 22.13460937069465 26.681521604173902 29.286757172896465 21.89711185223498 48-49 22.05209818903221 26.925099392094843 28.70366681804541 22.319135600827536 50-51 21.93804888445366 27.228254266085838 28.67567921079487 22.158017638665633 52-53 22.370813000033195 27.01450718719915 28.447166616870827 22.167513195896824 54-55 21.685312688811724 27.117313910485812 28.63783338755619 22.559540013146275 56-57 21.973330571313234 27.38622601822933 28.165633564918096 22.474809845539337 58-59 22.181374176362638 26.85741678640006 28.232934340067615 22.728274697169688 60-61 21.785008847031015 27.054586015383542 28.92391553326185 22.23648960432359 62-63 21.789338938780496 27.076049328289137 28.982162514590144 22.152449218340223 64-65 21.504086983599613 27.13775700922169 28.727135831535673 22.631020175643023 66-67 21.636230243436056 27.27672302467558 28.40310859844172 22.683938133446645 68-69 21.826833825327963 26.911840670759318 28.820593925825005 22.440731578087714 70-71 21.85643771438133 26.956771163246522 28.489493904034184 22.69729721833796 72-73 21.74173141352427 27.16360294849952 28.446433954221504 22.6482316837547 74-75 21.54653727510182 27.83664846101853 28.073348444950817 22.54346581892883 76-77 21.7816378522187 27.416605859649657 28.172317016248922 22.629439271882728 78-79 21.803166000856752 27.595944702909307 28.04716790032444 22.553721395909506 80-81 21.89148357577589 27.848630844271195 27.753269737126086 22.506615842826832 82-83 21.99365124050458 27.584038732611788 27.84238450961111 22.579925517272518 84-85 21.830076903361515 27.635942062858636 27.643710749870852 22.890270283908993 86-87 22.030112530243997 27.725943572868445 27.443953989286126 22.799989907601432 88-89 21.871412512955622 27.643129321168608 27.38730352439375 23.098154641482026 90-91 21.795089728589776 27.58651142839309 27.655436371918462 22.96296247109867 92-93 21.96557047759757 27.442487425090057 27.75869453344179 22.833247563870582 94-95 21.611974309653267 27.33725916959865 27.86717402866095 23.183592492087133 96-97 21.665927458442777 27.277912192686355 27.92002740824744 23.13613294062343 98-99 21.76684978376598 27.584448151650708 27.206370668678474 23.442331395904837 100-101 21.770506395119053 27.347993450441564 27.41348903480366 23.468011119635722 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 351.0 1 269.5 2 290.0 3 378.0 4 325.5 5 323.5 6 492.0 7 904.5 8 1458.5 9 1730.5 10 1535.0 11 1288.0 12 1288.5 13 1378.5 14 1436.5 15 1485.0 16 1570.5 17 1671.0 18 1780.5 19 1888.0 20 2067.5 21 2335.5 22 2686.0 23 3145.0 24 3653.0 25 4288.5 26 5090.0 27 6013.0 28 7020.0 29 8196.0 30 9687.0 31 11316.5 32 13546.0 33 14880.5 34 15049.5 35 15804.0 36 17558.5 37 19286.5 38 19742.5 39 19898.0 40 20387.0 41 21389.0 42 22137.0 43 22709.0 44 23469.0 45 24754.0 46 25337.0 47 25865.0 48 28351.5 49 28205.0 50 25722.0 51 24378.5 52 23778.0 53 24256.5 54 24509.0 55 23033.5 56 20735.5 57 18997.5 58 18418.5 59 16682.0 60 13615.5 61 11569.5 62 10107.0 63 7819.0 64 5512.0 65 4292.0 66 3285.5 67 2568.5 68 1953.0 69 1360.0 70 1064.0 71 803.0 72 637.5 73 586.5 74 497.0 75 401.0 76 294.0 77 178.0 78 118.0 79 97.0 80 79.5 81 58.0 82 41.0 83 30.0 84 19.0 85 12.5 86 10.0 87 6.0 88 3.0 89 5.5 90 5.0 91 2.5 92 1.5 93 1.0 94 1.5 95 1.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01434102968593145 2 0.005975429035804771 3 0.0074360894667792705 4 0.03704766002198958 5 0.047803432286438165 6 0.06772152907245407 7 0.03332961528859994 8 0.033064040664786395 9 0.020449246033642995 10-11 0.054310010569870025 12-13 0.050658359492433774 14-15 0.022573843024151358 16-17 0.024964014638473267 18-19 0.030939443674278033 20-21 0.02376892883131231 22-23 0.016598413988346584 24-25 0.004846736884597203 26-27 0.0037844383893430216 28-29 0.0024565652702752947 30-31 0.007303302154872498 32-33 9.295111833474088E-4 34-35 0.00272213989408884 36-37 0.004448374948876886 38-39 0.005842641723897998 40-41 0.0020582033345549764 42-43 5.975429035804771E-4 44-45 0.0015934477428812721 46-47 0.0031205018298091584 48-49 7.967238714406361E-4 50-51 0.0029877145179023853 52-53 0.0011950858071609542 54-55 0.002522958926228681 56-57 0.0015934477428812721 58-59 0.002921320861948999 60-61 8.631175273940224E-4 62-63 9.295111833474088E-4 64-65 0.0029877145179023853 66-67 0.001527054086927886 68-69 0.0035852574214828626 70-71 0.005975429035804771 72-73 0.003054108173855772 74-75 0.0027885335500422263 76-77 0.0024565652702752947 78-79 0.0320681358254856 80-81 0.020781214313409926 82-83 0.0031205018298091584 84-85 0.008100026026313134 86-87 0.005577067100084453 88-89 0.004315587636970112 90-91 0.012083645383516315 92-93 0.012813975599003564 94-95 0.005112311508410749 96-97 0.0034524701095760895 98-99 0.026026313133727445 100-101 0.048002613254298325 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 753084.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.2705955666293 #Duplication Level Percentage of deduplicated Percentage of total 1 82.61851140215096 50.62085398438142 2 10.248931539214711 12.55916278658592 3 2.9791383730125016 5.476007471696249 4 1.275064193927991 3.1249577019060846 5 0.7183311123184765 2.200628753289617 6 0.41458191273809064 1.524100842276909 7 0.2976341906160236 1.27653568840248 8 0.21369981084014464 1.0474811746121349 9 0.16481878483710755 0.9088690596783938 >10 0.9625079250466387 10.916974575405195 >50 0.06606143905975208 2.7821039372430785 >100 0.036387474086224406 4.407872115417071 >500 0.0032488816135314742 1.309820796923466 >1k 8.663684302750598E-4 1.1419460949874798 >5k 2.1659210756876495E-4 0.7026850171945038 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5295 0.7031088165463614 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4097 0.5440296168820477 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2432 0.3229387425572712 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1061 0.1408873379330858 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1015 0.13477912158537428 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 961 0.12760860674240854 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 786 0.10437082715872334 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.327873119067727E-4 0.0 20-21 0.0 0.0 0.0 3.319682797669317E-4 0.0 22-23 0.0 0.0 0.0 7.303302154872497E-4 0.0 24-25 0.0 0.0 0.0 9.295111833474088E-4 0.0 26-27 0.0 0.0 0.0 0.0015270540869278858 0.0 28-29 0.0 0.0 0.0 0.0025893525821820674 0.0 30-31 0.0 0.0 0.0 0.004846736884597204 0.0 32-33 0.0 0.0 0.0 0.007236908498919111 0.0 34-35 0.0 0.0 0.0 0.010822165920401973 0.0 36-37 0.0 0.0 0.0 0.014540210653791609 0.0 38-39 0.0 0.0 0.0 0.024233684422986013 0.0 40-41 0.0 0.0 0.0 0.037645202925570054 0.0 42-43 0.0 0.0 0.0 0.060351833261628185 0.0 44-45 0.0 0.0 0.0 0.08544863521200823 0.0 46-47 0.0 0.0 0.0 0.13730208051160295 0.0 48-49 0.0 0.0 0.0 0.18968667505882478 0.0 50-51 0.0 0.0 0.0 0.2255392492736534 0.0 52-53 0.0 0.0 0.0 0.27480334199106604 0.0 54-55 0.0 0.0 0.0 0.32559448879540664 0.0 56-57 0.0 0.0 0.0 0.42166610895995665 0.0 58-59 0.0 0.0 0.0 0.5498058649499923 0.0 60-61 0.0 0.0 0.0 0.6757546302935662 0.0 62-63 0.0 0.0 0.0 0.8038943862836019 0.0 64-65 0.0 0.0 0.0 0.9617121064848011 0.0 66-67 0.0 0.0 0.0 1.1678644082200658 0.0 68-69 0.0 0.0 0.0 1.41126355094518 0.0 70-71 0.0 0.0 0.0 1.611971572892267 0.0 72-73 0.0 0.0 0.0 1.776627839656665 0.0 74-75 0.0 0.0 0.0 1.959276787184431 0.0 76-77 0.0 0.0 0.0 2.1853471857057114 0.0 78-79 0.0 0.0 0.0 2.4232356549866947 0.0 80-81 0.0 0.0 0.0 2.712512813975599 0.0 82-83 0.0 0.0 0.0 3.0111514784539306 0.0 84-85 0.0 0.0 0.0 3.422858539020879 0.0 86-87 0.0 0.0 0.0 3.9044117256507906 0.0 88-89 0.0 0.0 0.0 4.508593994826606 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3740 0.0 37.105457 1 GTATCAA 5930 0.0 35.18329 1 TCGCGAT 30 0.003957672 31.656307 52-53 ATCAACG 7285 0.0 28.44164 3 TCAACGC 7325 0.0 28.223326 4 CAACGCA 7370 0.0 28.117353 5 TATCAAC 7415 0.0 27.880764 2 AACGCAG 7505 0.0 27.674906 6 TACGGGT 70 0.008367841 27.157421 4 GTACATG 4610 0.0 25.360674 1 ACGCAGA 8320 0.0 25.07488 7 GAGTACT 4530 0.0 24.862675 12-13 CGCAGAG 8420 0.0 24.777079 8 TCTACAC 365 0.0 24.73765 3 TAAGGTG 350 0.0 24.443302 5 TACATGG 4805 0.0 24.13365 2 GCAGAGT 8675 0.0 23.774858 9 GTAAGGT 400 0.0 23.762743 4 GGTAAGG 360 0.0 23.761166 3 ACATGGG 4725 0.0 23.534868 3 >>END_MODULE