FastQCFastQC Report
Thu 26 May 2016
SRR936738_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936738_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753084
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59170.785702524552374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51100.6785431638436085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32850.43620631961374823No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14150.18789404634808335No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13500.17926287107414313No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12120.1609382220310085No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC11320.1503152370784667No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA10440.13862995363067068No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA9570.12707745749478144No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9110.12096924114706992No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA7560.10038720780152016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAG3700.035.9510462
ATCCGCT550.002581469534.5388877
GGTATCA44800.034.2633671
GTATCAA67150.032.074391
GTAAGGT4800.028.6962724
TGAACCG1503.3396645E-828.4945835
TAAGGTG5100.027.0046675
GGTAAGG5350.025.746193
ATCAACG86150.024.8099523
TATCAAC87200.024.6250532
TCAACGC87300.024.4831354
CAACGCA89000.024.0656495
AACGCAG90700.023.6145846
AAGGTAA5100.023.5140761
GGTGTGC8750.022.252918
GTACATG49150.021.7640361
GAGTACT56250.021.5292412-13
AGGCGAC2901.05501385E-1021.2890575
ACGCAGA101900.021.065677
AGTACTT57300.021.05184412-13
Produced by FastQC (version 0.11.5)