##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936738_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 753084 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.419172363242346 31.0 31.0 34.0 30.0 34.0 2 31.64995671133632 31.0 31.0 34.0 30.0 34.0 3 31.855198092111902 31.0 31.0 34.0 30.0 34.0 4 35.69752245433445 37.0 35.0 37.0 33.0 37.0 5 35.49949673608787 37.0 35.0 37.0 33.0 37.0 6 35.6076838705908 37.0 35.0 37.0 33.0 37.0 7 35.5356972130599 37.0 35.0 37.0 33.0 37.0 8 35.49849020826362 37.0 35.0 37.0 33.0 37.0 9 37.10495774707735 39.0 37.0 39.0 33.0 39.0 10-11 36.99443090013863 39.0 37.0 39.0 33.0 39.0 12-13 36.85832788905354 39.0 37.0 39.0 32.5 39.0 14-15 37.918263434092346 40.0 37.0 41.0 33.0 41.0 16-17 37.84526958480063 40.0 37.0 41.0 33.0 41.0 18-19 37.93019437406717 39.5 37.0 41.0 33.0 41.0 20-21 38.011379872630414 40.0 37.0 41.0 33.0 41.0 22-23 37.907852244902294 40.0 37.0 41.0 33.0 41.0 24-25 37.77548786058395 40.0 37.0 41.0 32.0 41.0 26-27 37.52767685942072 39.0 36.5 41.0 32.0 41.0 28-29 37.4016404544513 39.0 36.0 41.0 32.0 41.0 30-31 37.31204752723468 39.0 36.0 41.0 32.0 41.0 32-33 37.04984038965109 39.0 35.5 41.0 31.0 41.0 34-35 36.84066916837962 39.0 35.0 41.0 31.0 41.0 36-37 36.55798755517313 39.0 35.0 41.0 30.0 41.0 38-39 36.32745882265458 39.0 35.0 40.0 30.0 41.0 40-41 36.02845964062442 38.5 35.0 40.0 30.0 41.0 42-43 35.70436232877076 38.0 35.0 40.0 28.5 41.0 44-45 35.30820731817433 38.0 34.5 40.0 27.0 41.0 46-47 34.75745999118293 38.0 34.0 40.0 25.0 41.0 48-49 34.103455922579684 38.0 33.0 40.0 23.0 41.0 50-51 33.876511783546064 38.0 33.0 40.0 22.0 41.0 52-53 33.58488229732673 38.0 33.0 40.0 20.0 41.0 54-55 33.27735020263344 37.0 33.0 40.0 18.5 41.0 56-57 32.984485794413374 37.0 32.0 40.0 18.0 41.0 58-59 32.57567681692879 36.5 31.5 39.5 14.0 41.0 60-61 32.181386804128095 36.0 31.0 39.0 12.0 40.5 62-63 31.869901631159337 35.5 31.0 39.0 8.5 40.0 64-65 31.43344035990673 35.0 30.5 38.5 7.5 40.0 66-67 31.01457274354521 35.0 30.0 38.0 6.5 40.0 68-69 30.546927699964414 34.5 29.0 37.5 2.0 40.0 70-71 30.05163501017151 34.0 29.0 37.0 2.0 39.0 72-73 29.635887231703236 34.0 29.0 36.0 2.0 39.0 74-75 29.265073351711095 34.0 28.5 36.0 2.0 39.0 76-77 27.8787884220087 32.0 26.5 34.5 2.0 36.5 78-79 28.671260443722083 33.5 28.0 35.0 2.0 37.0 80-81 28.519819834175205 34.0 28.0 35.0 2.0 37.0 82-83 28.174528339468107 33.5 27.5 35.0 2.0 36.5 84-85 27.72722564813487 33.0 26.5 35.0 2.0 36.0 86-87 27.312589963403816 33.0 26.0 35.0 2.0 35.5 88-89 26.875665264432655 33.0 25.0 35.0 2.0 35.0 90-91 26.412029069798322 32.5 25.0 35.0 2.0 35.0 92-93 25.9198967180288 32.0 24.0 34.0 2.0 35.0 94-95 25.364209703034454 32.0 21.5 34.0 2.0 35.0 96-97 24.673991214791442 32.0 17.0 34.0 2.0 35.0 98-99 23.782478448619276 31.0 2.0 34.0 2.0 35.0 100-101 21.19953418210983 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.48380764661031606 1101 2 0.4799154997127282 1101 3 0.41082348402937185 1101 4 0.29011835378911144 1101 5 0.46474727116639514 1101 6 0.2504940922455816 1101 7 0.32678087512839227 1101 8 0.2889395122353804 1101 9 0.5828289436017613 1101 10-11 0.5423132690747252 1101 12-13 0.3748941128893648 1101 14-15 0.6345779813187136 1101 16-17 0.46885376384570776 1101 18-19 0.5306092617981761 1101 20-21 0.3756854604460358 1101 22-23 0.4244009927570218 1101 24-25 0.3177644730614233 1101 26-27 0.3507113244742115 1101 28-29 0.4592883353777921 1101 30-31 0.34232388974432126 1101 32-33 0.4393013386526263 1101 34-35 0.6047184782463546 1101 36-37 0.5790209801383597 1101 38-39 0.7654205483868779 1101 40-41 0.6903272548531376 1101 42-43 0.8363501433784393 1101 44-45 1.0855944396250123 1101 46-47 1.1879147895073672 1101 48-49 1.095221946370863 1101 50-51 1.070912151616895 1101 52-53 1.0164838564546557 1101 54-55 0.9800471509558193 1101 56-57 0.9327946271298018 1101 58-59 1.0443805133980675 1101 60-61 1.205181853849144 1101 62-63 1.1820883279362207 1101 64-65 1.2406927190470647 1101 66-67 0.9760315812300213 1101 68-69 0.7940300104663329 1101 70-71 0.8460537855085271 1101 72-73 0.832996629751058 1101 74-75 0.8104255457020777 1101 76-77 1.2151541381484634 1101 78-79 1.1549019943726826 1101 80-81 0.9922414851455557 1101 82-83 0.8879215580332023 1101 84-85 1.041673239704398 1101 86-87 1.074183128089441 1101 88-89 1.0119758345910306 1101 90-91 0.9121512419801441 1101 92-93 1.0589630600592557 1101 94-95 0.8862070870076977 1101 96-97 1.0570510170626406 1101 98-99 0.9812870190686098 1101 100-101 0.7748712533916269 1102 1 0.38125846968559785 1102 2 0.5501700527160835 1102 3 0.2896877860144045 1102 4 0.12831420867650678 1102 5 0.17987457572107957 1102 6 0.1205778024243358 1102 7 0.16762773936304853 1102 8 0.058636932480681025 1102 9 0.20540796892801438 1102 10-11 0.20341258233706583 1102 12-13 0.23342769795169716 1102 14-15 0.41815462774980716 1102 16-17 0.6080289492760969 1102 18-19 0.340306064877268 1102 20-21 0.38586519607447656 1102 22-23 0.20729735372069769 1102 24-25 0.2063940274674323 1102 26-27 0.04541884380274297 1102 28-29 0.14291281218917362 1102 30-31 0.28399747615320337 1102 32-33 0.4457514267390792 1102 34-35 0.5902480348966037 1102 36-37 0.5768146109831989 1102 38-39 0.739667886156333 1102 40-41 0.7360284117592286 1102 42-43 0.7787243536862363 1102 44-45 0.8094188782843048 1102 46-47 0.8611119628861843 1102 48-49 0.9504922651652521 1102 50-51 0.9726873460242444 1102 52-53 0.8179471014023108 1102 54-55 0.9148898296815418 1102 56-57 0.8729561563554213 1102 58-59 0.9549075452775071 1102 60-61 1.0729638146422538 1102 62-63 0.960665226844398 1102 64-65 0.8968554040566588 1102 66-67 1.002736643114961 1102 68-69 0.8306945918593307 1102 70-71 0.846079033216597 1102 72-73 1.0346690098805524 1102 74-75 0.5227671864664707 1102 76-77 0.626350935616955 1102 78-79 1.066410881565922 1102 80-81 1.0501457009517416 1102 82-83 0.9802685569223009 1102 84-85 1.1496303354324837 1102 86-87 0.8522368383804029 1102 88-89 0.9205461690951076 1102 90-91 0.959105667061781 1102 92-93 1.153043033577216 1102 94-95 0.9282823925049648 1102 96-97 1.0275245238009738 1102 98-99 0.6185041044731925 1102 100-101 0.25658139287118686 1103 1 0.46631295959945973 1103 2 0.5692071223696047 1103 3 0.4182791667007564 1103 4 0.2534380493898283 1103 5 0.16941347546514152 1103 6 0.21433554246891617 1103 7 0.25354911427017157 1103 8 0.22727702384760562 1103 9 0.08967538230831451 1103 10-11 0.21885483128441763 1103 12-13 0.2657638842081269 1103 14-15 0.21575934195296753 1103 16-17 0.22803060048561719 1103 18-19 0.46281377052164885 1103 20-21 0.11796333295974648 1103 22-23 0.14303606357566423 1103 24-25 0.09300753436122733 1103 26-27 0.2993232662922125 1103 28-29 0.5250302131854099 1103 30-31 0.5072238019368314 1103 32-33 0.4120237524676682 1103 34-35 0.45807043355361543 1103 36-37 0.5082842562483592 1103 38-39 0.7468386074596864 1103 40-41 0.7536464732392645 1103 42-43 0.7329806902027372 1103 44-45 0.9041415438868583 1103 46-47 0.864232060857951 1103 48-49 0.9525665971134032 1103 50-51 0.6065608771357844 1103 52-53 0.8009912437365685 1103 54-55 0.8964375633964892 1103 56-57 0.7216279647050428 1103 58-59 0.6030898388075201 1103 60-61 0.6955007604550651 1103 62-63 0.5358554599401444 1103 64-65 0.6313047944851498 1103 66-67 0.7166336351616351 1103 68-69 0.5920346171778768 1103 70-71 0.5079130453805085 1103 72-73 0.6505391972928294 1103 74-75 0.974706669637893 1103 76-77 0.8424495403449228 1103 78-79 0.9566948896018097 1103 80-81 0.9593240178188474 1103 82-83 0.913234610806775 1103 84-85 1.156274733704219 1103 86-87 0.9962945481528145 1103 88-89 0.8211106763466702 1103 90-91 0.5562191464354207 1103 92-93 0.6942613509947222 1103 94-95 0.61680751840969 1103 96-97 0.579671784169193 1103 98-99 0.2636127402989352 1103 100-101 0.5039549377428081 1104 1 0.2761180231915219 1104 2 0.42016219190484705 1104 3 0.09050845470741109 1104 4 0.0032948186754566677 1104 5 -0.06786989550835187 1104 6 0.03195292033650077 1104 7 -0.012542054159638383 1104 8 -0.11763442272317803 1104 9 -0.04389765941282775 1104 10-11 -0.06133089318961282 1104 12-13 0.0911011598529683 1104 14-15 0.169629839991714 1104 16-17 0.01541025102644511 1104 18-19 0.09113511843560218 1104 20-21 0.014560563436390339 1104 22-23 -0.07832522252916618 1104 24-25 0.054847543870323534 1104 26-27 0.21712726011130457 1104 28-29 0.07038294110648025 1104 30-31 0.2763662006055583 1104 32-33 0.27030676685016886 1104 34-35 0.29205890377826904 1104 36-37 0.14088231877064317 1104 38-39 0.23880372097448088 1104 40-41 0.22065318903624132 1104 42-43 0.3091711097914711 1104 44-45 0.7091225257007139 1104 46-47 0.8886701328893238 1104 48-49 0.845832376264724 1104 50-51 0.6431789087535194 1104 52-53 0.6806994334429746 1104 54-55 0.8485236206887734 1104 56-57 1.033985922627302 1104 58-59 0.9411330457491545 1104 60-61 0.6328411859869796 1104 62-63 0.5563053589060303 1104 64-65 0.603251484132123 1104 66-67 0.7287292015469902 1104 68-69 0.7554364952237584 1104 70-71 0.43767739816786033 1104 72-73 0.6810512025228306 1104 74-75 0.6689180092313798 1104 76-77 0.7320578845740897 1104 78-79 0.7641330804968511 1104 80-81 0.8562142472257186 1104 82-83 0.733614380211268 1104 84-85 0.8372262373295634 1104 86-87 0.7539633345548076 1104 88-89 0.6804515773332405 1104 90-91 0.5361329702794713 1104 92-93 0.7643730828383006 1104 94-95 0.8245107752740672 1104 96-97 0.8602551075972222 1104 98-99 0.6096774615636456 1104 100-101 0.6993020196398696 1105 1 0.3674790498716973 1105 2 0.37257016218515915 1105 3 0.1687883151149343 1105 4 0.21026093372667987 1105 5 -0.06804551185418006 1105 6 0.08940648332198009 1105 7 0.16438015680512308 1105 8 0.1269045843690222 1105 9 -0.04734244158101575 1105 10-11 0.20379800470524145 1105 12-13 0.10091766328649499 1105 14-15 0.203818740138054 1105 16-17 0.16824150173003005 1105 18-19 0.06053959890390814 1105 20-21 0.2193832584718507 1105 22-23 0.19172993901535307 1105 24-25 -0.06792179081390515 1105 26-27 0.08903314776187443 1105 28-29 -0.04071015807172529 1105 30-31 0.004732743440186482 1105 32-33 0.30523190475403794 1105 34-35 0.17707297560085067 1105 36-37 0.4324887580366621 1105 38-39 0.21853579347251184 1105 40-41 0.14327617676561033 1105 42-43 0.288058872934549 1105 44-45 -0.014324507959081245 1105 46-47 0.31348607344994406 1105 48-49 0.656367105522861 1105 50-51 0.52098145293904 1105 52-53 0.5949221059635192 1105 54-55 0.7056214479993699 1105 56-57 0.40441483177961857 1105 58-59 0.585957992276235 1105 60-61 0.717855257809564 1105 62-63 0.47416869336510814 1105 64-65 0.46772294647592716 1105 66-67 0.44940239753933753 1105 68-69 0.2561119427077152 1105 70-71 0.4778980443459524 1105 72-73 0.14730584622428466 1105 74-75 0.22997846178119374 1105 76-77 0.18344818064523594 1105 78-79 0.2027979459702287 1105 80-81 0.2599066934713541 1105 82-83 0.6674903230233795 1105 84-85 0.46686487292564394 1105 86-87 0.5525584037881757 1105 88-89 0.39461796256345494 1105 90-91 0.11513330800321242 1105 92-93 0.514637633102847 1105 94-95 0.5688178787301048 1105 96-97 0.37819274127953406 1105 98-99 0.07999379613529811 1105 100-101 0.4585860453068733 1106 1 0.28467132695843134 1106 2 0.4322342740920142 1106 3 0.05891130655718513 1106 4 -0.09441022820042377 1106 5 -0.06401652998878404 1106 6 -0.0814608785744042 1106 7 -0.1433734694181581 1106 8 0.028311796330321215 1106 9 -0.037441700281860335 1106 10-11 -0.08220665808042327 1106 12-13 0.172937459184908 1106 14-15 0.2099599359890192 1106 16-17 0.14787811157771813 1106 18-19 -0.08806714957091799 1106 20-21 -0.16223503528364347 1106 22-23 0.10049081070498289 1106 24-25 0.07701057087307817 1106 26-27 0.13303923451201882 1106 28-29 0.14426213046257175 1106 30-31 0.030202163104860347 1106 32-33 0.09791244809420618 1106 34-35 -0.1858488040755475 1106 36-37 0.1951062002055508 1106 38-39 0.4993527581944832 1106 40-41 0.38257201236751825 1106 42-43 0.16159686626146197 1106 44-45 0.227554008325761 1106 46-47 0.5812311838071977 1106 48-49 0.2625690292709493 1106 50-51 0.350155274329218 1106 52-53 0.5963307139684417 1106 54-55 0.5302504494093725 1106 56-57 0.35024764159660293 1106 58-59 0.09135928501261503 1106 60-61 0.5381788607189186 1106 62-63 0.19205340808359495 1106 64-65 0.42476947660953 1106 66-67 0.4834302625698399 1106 68-69 0.39998261322746487 1106 70-71 0.6665035363815228 1106 72-73 0.4271630309627348 1106 74-75 0.726493936924637 1106 76-77 0.7417895412293412 1106 78-79 0.7108691721153342 1106 80-81 0.33601270902328295 1106 82-83 0.4170910545502302 1106 84-85 0.7576091037754118 1106 86-87 0.7752915779725384 1106 88-89 0.7023311035219741 1106 90-91 0.3552179082461251 1106 92-93 0.7266422121258991 1106 94-95 0.5347802413943157 1106 96-97 0.5162726228950341 1106 98-99 0.3588842206721594 1106 100-101 0.7602361688567321 1107 1 0.4381637812258816 1107 2 0.4325489096116044 1107 3 0.3107577202642524 1107 4 0.11980688134794093 1107 5 -0.14859276562319224 1107 6 0.01603197312739013 1107 7 0.039981759603378464 1107 8 0.37362703904126704 1107 9 0.3267958329756695 1107 10-11 0.1358550767204747 1107 12-13 0.16650399079750144 1107 14-15 0.3240097919190674 1107 16-17 0.18503546245704428 1107 18-19 -0.00848920276087739 1107 20-21 0.1389787954314059 1107 22-23 0.2537321085489026 1107 24-25 0.1739955898064025 1107 26-27 0.14641952397669655 1107 28-29 0.3127411713210293 1107 30-31 0.5036679009527489 1107 32-33 0.4340867009728484 1107 34-35 0.1188398171584879 1107 36-37 0.1931604279133481 1107 38-39 0.1904248373280808 1107 40-41 0.3186154431090671 1107 42-43 0.3733934902775431 1107 44-45 0.3396415321994297 1107 46-47 0.5736109323195748 1107 48-49 0.9196577123281102 1107 50-51 0.7178179188131608 1107 52-53 0.6411276360602614 1107 54-55 0.8969167480812743 1107 56-57 1.1777694176865054 1107 58-59 1.0146378975251764 1107 60-61 0.8895384173588319 1107 62-63 0.8083333122577585 1107 64-65 0.9230859005836507 1107 66-67 0.7616536206692182 1107 68-69 0.6441839358006298 1107 70-71 0.7721410321035833 1107 72-73 0.4311704938964631 1107 74-75 0.8484648800584047 1107 76-77 0.7086313734016514 1107 78-79 0.5492403537097061 1107 80-81 0.7814924453918373 1107 82-83 0.9197240570562748 1107 84-85 0.658509954947295 1107 86-87 0.9864230514603562 1107 88-89 1.1513711891911562 1107 90-91 1.0025942896064528 1107 92-93 1.1815278945537777 1107 94-95 1.102862951896519 1107 96-97 0.7937314718810278 1107 98-99 0.8144019340999193 1107 100-101 0.6344012807455393 1108 1 0.23598364469208022 1108 2 0.16842646962041385 1108 3 -0.18407161248183712 1108 4 0.02936424756683209 1108 5 -0.16000875329110187 1108 6 -0.1935342017239634 1108 7 -0.1847704976086888 1108 8 0.049711601912882486 1108 9 -0.09496148920006675 1108 10-11 -0.14986392790405745 1108 12-13 -0.12621127015823674 1108 14-15 -0.1960364534013408 1108 16-17 -0.2626412607316766 1108 18-19 -0.27878649410639866 1108 20-21 -0.030792180124635138 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-0.8536894262576027 2316 100-101 -1.9212142619127874 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 16.0 5 40.0 6 100.0 7 217.0 8 538.0 9 1034.0 10 1521.0 11 2497.0 12 3340.0 13 4580.0 14 5763.0 15 7731.0 16 10020.0 17 12374.0 18 10472.0 19 6843.0 20 5969.0 21 5954.0 22 6304.0 23 7029.0 24 8088.0 25 9381.0 26 11248.0 27 14096.0 28 16891.0 29 20498.0 30 24830.0 31 29883.0 32 36384.0 33 45196.0 34 58982.0 35 76799.0 36 103239.0 37 125860.0 38 74730.0 39 4630.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.87479133005719 19.511125711489083 15.403294471000745 21.210788487452987 2 16.11465543343727 21.162968589808287 39.13684389458755 23.585532082166893 3 19.42312008084446 25.698259480433595 30.47628912251627 24.402331316205675 4 13.038200784865769 17.577540355513648 36.41445162318475 32.969807236435834 5 12.994566343196775 38.44285630819404 33.201209957986094 15.36136739062309 6 31.089227762108873 36.86202335994391 16.643296099771074 15.40545277817614 7 28.000993249093064 34.52629454350378 19.993785553802763 17.478926653600396 8 27.55363279527915 33.46001242889239 19.02377424032379 19.96258053550467 9 27.44012238458719 16.462825150457615 20.158398801650918 35.93865366330428 10-11 25.443859661935658 26.985591864891116 25.924352145152945 21.646196328020274 12-13 26.936455253633895 24.777464915909057 27.083450905510475 21.20262892494657 14-15 23.219501183469273 25.664253252465702 26.72960797787759 24.386637586187437 16-17 21.400600729799066 28.948032357612163 27.474757132006523 22.176609780582247 18-19 21.310504538670322 26.20789978275996 31.534861980868005 20.946733697701717 20-21 22.27422324350487 24.995169858322484 32.02201349518347 20.708593402989177 22-23 21.868599181359155 25.014025780879123 32.182411504792 20.934963532969714 24-25 21.77539291765593 25.36616101258293 32.15278773682617 20.70565833293497 26-27 21.222798519155898 25.768705748628307 31.96894370349124 21.03955202872455 28-29 21.042008593994826 26.13128150378975 31.83927689341428 20.987433008801144 30-31 21.248227289386044 25.655703746195645 31.877386851931522 21.218682112486785 32-33 21.013880266517052 25.792529687702302 31.644086196421874 21.54950384935877 34-35 21.689857125879143 25.67372062960487 31.493389441032143 21.143032803483848 36-37 22.041301961919547 25.454626336291692 31.34330637456204 21.16076532722672 38-39 21.547729071391768 25.699656346436782 31.30480796298952 21.447806619181925 40-41 21.490334339239293 25.90104939562647 31.230823880141156 21.37779238499308 42-43 21.545907705833176 26.05656482218662 30.71948068677792 21.67804678520228 44-45 21.603504620463358 26.213079118214544 30.580310013903215 21.603106247418875 46-47 22.258946290717848 26.736722733209085 29.607728963095408 21.39660201297766 48-49 22.3771162355841 26.92599350852869 28.810618495912287 21.886271759974925 50-51 22.135828862803418 27.22466404631646 28.8923487544484 21.74715833643172 52-53 22.590302450049233 27.194610303339743 28.59521702266666 21.619870223944364 54-55 21.85223870388975 27.27308342790058 28.80762930258841 22.06704856562126 56-57 22.32572839611097 27.345836849629134 28.39557979208317 21.93285496217672 58-59 22.38352502657457 26.924739350086547 28.358205982316377 22.33352964102251 60-61 22.064443989981754 26.97078188535323 29.1603477170775 21.80442640758752 62-63 21.99155825659474 27.143916652889246 29.134173523840257 21.73035156667576 64-65 21.741099592915536 27.211903070067674 28.87231626412012 22.174681072896675 66-67 21.874790435422902 27.288445181890435 28.563593761100698 22.273170621585965 68-69 22.204207374358052 27.03920221224102 28.684772252708036 22.071818160692885 70-71 22.150950712220823 27.237417122248086 28.255852400915195 22.355779764615896 72-73 22.129167701302514 27.24971882249367 28.25916930803112 22.3619441681727 74-75 21.970922234438657 27.77552039347536 27.960625906273407 22.29293146581258 76-77 22.09349728444899 27.441008140013544 28.16125990943737 22.304234666100097 78-79 22.149319539298723 27.55542127473082 27.927076658488083 22.36818252748238 80-81 22.18330833149057 28.0114271370599 27.555018995423964 22.250245536025563 82-83 22.260227353468306 27.735954425716347 27.529770501687658 22.474047719127693 84-85 21.85159362549801 27.892895086321378 27.48990703851262 22.765604249667994 86-87 22.00620065061014 27.782208289535454 27.218120402052893 22.993470657801513 88-89 21.7310334888126 27.792931637606454 27.01321075642888 23.462824117152067 90-91 21.83766365728965 27.823698412339997 27.341077929892187 22.99756000047817 92-93 21.834919644250274 27.36656895389794 27.663757415602397 23.13475398624939 94-95 21.335098697490597 27.413453774727493 27.634633363788964 23.616814163992945 96-97 21.567932173407225 27.375199042735588 27.667305462702146 23.389563321155038 98-99 21.387781446135783 27.565983962517233 27.11033140058381 23.935903190763174 100-101 21.075481307799933 27.458831607179263 27.285054141939735 24.180632943081072 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 563.0 1 420.5 2 378.5 3 460.0 4 398.5 5 389.5 6 550.0 7 1032.0 8 1616.5 9 1879.5 10 1722.5 11 1464.0 12 1426.0 13 1481.0 14 1632.0 15 1760.5 16 1801.5 17 1911.5 18 2057.0 19 2214.5 20 2369.5 21 2650.0 22 3012.5 23 3359.5 24 3761.0 25 4370.0 26 5201.5 27 6172.0 28 7278.5 29 8574.0 30 9964.0 31 11528.0 32 13543.5 33 14824.5 34 14982.5 35 15506.5 36 17267.0 37 19127.5 38 19763.5 39 19839.5 40 20192.0 41 21207.0 42 21862.5 43 22352.0 44 23300.5 45 24565.0 46 24732.0 47 25091.0 48 27992.5 49 27982.0 50 25450.0 51 24040.5 52 23389.5 53 23858.5 54 24308.5 55 23051.5 56 20561.0 57 19033.0 58 18658.5 59 16969.5 60 13666.0 61 11349.5 62 10149.5 63 7923.5 64 5457.0 65 4081.0 66 3050.5 67 2459.0 68 1912.0 69 1328.5 70 1048.5 71 832.0 72 673.5 73 601.0 74 515.0 75 428.5 76 309.5 77 199.5 78 137.0 79 106.5 80 88.0 81 61.5 82 47.0 83 39.5 84 23.5 85 11.5 86 7.5 87 4.5 88 3.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.967886716488466 2 0.010622984952541815 3 0.004381981292923498 4 0.01181807075970277 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0063737909715250885 10-11 3.319682797669317E-4 12-13 6.639365595338635E-5 14-15 1.9918096786015902E-4 16-17 0.0 18-19 0.0 20-21 6.639365595338635E-5 22-23 8.631175273940224E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.004846736884597203 34-35 0.0011286921512075679 36-37 0.00272213989408884 38-39 0.0 40-41 0.0041828003250633395 42-43 0.011884464415656154 44-45 0.0027885335500422263 46-47 0.00272213989408884 48-49 0.012747581943050177 50-51 5.311492476270908E-4 52-53 0.002323777958368522 54-55 0.012282826351376471 56-57 0.005643460756037839 58-59 0.0019254160226482037 60-61 0.007701664090592815 62-63 0.005377886132224294 64-65 0.022441055712244584 66-67 0.004581162260783657 68-69 1.9918096786015902E-4 70-71 0.0022573843024151358 72-73 1.327873119067727E-4 74-75 0.0 76-77 0.0018590223666948176 78-79 0.013477912158537428 80-81 0.01852383001099479 82-83 0.015336934525232247 84-85 0.011154134200168905 86-87 0.013079550222817109 88-89 0.033263221632646556 90-91 0.028549272059956127 92-93 0.003518863765529476 94-95 0.010091835704914724 96-97 0.013942667750211133 98-99 0.013013156566863722 100-101 0.014540210653791609 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 753084.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.678806777034524 #Duplication Level Percentage of deduplicated Percentage of total 1 81.26948773224319 47.68796567508875 2 10.879257890837888 12.767637433080093 3 3.308413789027837 5.824013203945232 4 1.4368143720608872 3.3724221165050796 5 0.7709769443326425 2.262000357302181 6 0.46865719056120353 1.6500146837765226 7 0.32194269125607716 1.322384908144568 8 0.22354970030469462 1.049410373539452 9 0.1798394628398055 0.9497488581776363 >10 1.0139845982161972 11.120188592527034 >50 0.08115591517872342 3.2235708114422645 >100 0.040918556265037376 4.765821548497336 >500 0.0031825543755553305 1.2749586418464123 >1k 0.001363951875237999 1.2589516281383595 >5k 4.5465062507933304E-4 1.4709111679891598 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5917 0.785702524552374 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5110 0.6785431638436085 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3285 0.43620631961374823 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1415 0.18789404634808335 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1350 0.17926287107414313 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1212 0.1609382220310085 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1132 0.1503152370784667 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1044 0.13862995363067068 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 957 0.12707745749478144 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 911 0.12096924114706992 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 756 0.10038720780152016 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.327873119067727E-4 0.0 20-21 0.0 0.0 0.0 3.319682797669317E-4 0.0 22-23 0.0 0.0 0.0 7.303302154872497E-4 0.0 24-25 0.0 0.0 0.0 9.295111833474088E-4 0.0 26-27 0.0 0.0 0.0 0.0015934477428812721 0.0 28-29 0.0 0.0 0.0 0.0028549272059956127 0.0 30-31 0.0 0.0 0.0 0.005245098820317521 0.0 32-33 0.0 0.0 0.0 0.007967238714406362 0.0 34-35 0.0 0.0 0.0 0.011552496135889222 0.0 36-37 0.0 0.0 0.0 0.01527054086927886 0.0 38-39 0.0 0.0 0.0 0.025229589262286807 0.0 40-41 0.0 0.0 0.0 0.03923865066845132 0.0 42-43 0.0 0.0 0.0 0.06300757949976364 0.0 44-45 0.0 0.0 0.0 0.08836995607395723 0.0 46-47 0.0 0.0 0.0 0.1404889759973655 0.0 48-49 0.0 0.0 0.0 0.19274078323268057 0.0 50-51 0.0 0.0 0.0 0.22879253841536934 0.0 52-53 0.0 0.0 0.0 0.2781230247887354 0.0 54-55 0.0 0.0 0.0 0.3286485969692624 0.0 56-57 0.0 0.0 0.0 0.4243218551980921 0.0 58-59 0.0 0.0 0.0 0.5539222716191023 0.0 60-61 0.0 0.0 0.0 0.6806013671781634 0.0 62-63 0.0 0.0 0.0 0.8083427612324787 0.0 64-65 0.0 0.0 0.0 0.9658285131539112 0.0 66-67 0.0 0.0 0.0 1.1724455704808494 0.0 68-69 0.0 0.0 0.0 1.4145832337428494 0.0 70-71 0.0 0.0 0.0 1.6151584683780296 0.0 72-73 0.0 0.0 0.0 1.780013916110288 0.0 74-75 0.0 0.0 0.0 1.9633268001975874 0.0 76-77 0.0 0.0 0.0 2.1910570401177027 0.0 78-79 0.0 0.0 0.0 2.429144690366546 0.0 80-81 0.0 0.0 0.0 2.7222062877447932 0.0 82-83 0.0 0.0 0.0 3.0217744634064725 0.0 84-85 0.0 0.0 0.0 3.4348757907484426 0.0 86-87 0.0 0.0 0.0 3.9152338915711926 0.0 88-89 0.0 0.0 0.0 4.519482554402962 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTAAG 370 0.0 35.951046 2 ATCCGCT 55 0.0025814695 34.538887 7 GGTATCA 4480 0.0 34.263367 1 GTATCAA 6715 0.0 32.07439 1 GTAAGGT 480 0.0 28.696272 4 TGAACCG 150 3.3396645E-8 28.494583 5 TAAGGTG 510 0.0 27.004667 5 GGTAAGG 535 0.0 25.74619 3 ATCAACG 8615 0.0 24.809952 3 TATCAAC 8720 0.0 24.625053 2 TCAACGC 8730 0.0 24.483135 4 CAACGCA 8900 0.0 24.065649 5 AACGCAG 9070 0.0 23.614584 6 AAGGTAA 510 0.0 23.514076 1 GGTGTGC 875 0.0 22.25291 8 GTACATG 4915 0.0 21.764036 1 GAGTACT 5625 0.0 21.52924 12-13 AGGCGAC 290 1.05501385E-10 21.289057 5 ACGCAGA 10190 0.0 21.06567 7 AGTACTT 5730 0.0 21.051844 12-13 >>END_MODULE