FastQCFastQC Report
Thu 26 May 2016
SRR936737_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936737_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833295
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55140.6617104386801793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44020.5282643001578072No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26850.32221482188180656No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12060.1447266574262416No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11050.13260609988059452No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10000.12000552025393167No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA8610.10332475293863518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61800.034.5990751
GGTATCA39250.033.6546861
GTACATG53900.028.0335961
TAAGGTG3950.027.6709025
ATCAACG76250.027.606053
TCAACGC76900.027.4377964
CAACGCA77150.027.4104825
TACATGG54300.027.1270352
TATCAAC78600.026.9015872
AACGCAG79150.026.77796
ACATGGG53650.025.950063
GTAAGGT3150.025.6466674
GTATAGG2600.025.5855711
CATGGGG20300.023.8779344
ACGCAGA88150.023.8282727
CATGGGT6000.023.7608834
CGCAGAG88750.023.667188
GAGTACT45800.023.13971712-13
GGTAAGG3500.023.079233
AGTCCGT1050.002189878622.6294146
Produced by FastQC (version 0.11.5)