##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936737_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 833295 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.393140484462286 31.0 31.0 34.0 30.0 34.0 2 31.426650825937994 31.0 31.0 34.0 28.0 34.0 3 31.437624130710013 31.0 31.0 34.0 28.0 34.0 4 35.108432187880645 37.0 35.0 37.0 32.0 37.0 5 35.0905021630995 37.0 35.0 37.0 32.0 37.0 6 35.203484960308174 37.0 35.0 37.0 32.0 37.0 7 35.08958892109037 37.0 35.0 37.0 32.0 37.0 8 35.03842456753011 37.0 35.0 37.0 32.0 37.0 9 36.541358102472714 39.0 37.0 39.0 32.0 39.0 10-11 36.41659676345112 39.0 37.0 39.0 32.0 39.0 12-13 36.31051308360185 39.0 35.0 39.0 32.0 39.0 14-15 37.31206115481312 39.5 37.0 41.0 32.0 41.0 16-17 37.19960698192117 39.0 36.0 41.0 31.0 41.0 18-19 37.2180044282037 39.0 36.5 41.0 31.0 41.0 20-21 37.16240587067006 39.0 36.5 41.0 31.0 41.0 22-23 37.01462627280855 39.0 36.0 41.0 31.0 41.0 24-25 37.08162355468352 39.0 36.0 41.0 31.0 41.0 26-27 37.02550657330237 39.0 36.0 41.0 31.0 41.0 28-29 36.901620674551026 39.0 36.0 41.0 30.5 41.0 30-31 36.65149496876856 39.0 36.0 41.0 30.0 41.0 32-33 36.40282372989158 39.0 35.0 40.0 30.0 41.0 34-35 36.13115343305792 38.5 35.0 40.0 30.0 41.0 36-37 35.91041227896483 38.0 35.0 40.0 29.0 41.0 38-39 35.67950545725104 38.0 35.0 40.0 28.0 41.0 40-41 35.421630995025765 38.0 35.0 40.0 27.0 41.0 42-43 35.13424657534246 38.0 34.0 40.0 26.0 41.0 44-45 34.76736209865654 38.0 34.0 40.0 24.5 41.0 46-47 34.3610078063591 38.0 33.0 40.0 23.5 41.0 48-49 34.1531192434852 38.0 33.0 40.0 22.5 41.0 50-51 33.17990627568868 37.0 32.0 39.5 20.5 40.5 52-53 33.288496870856065 37.5 33.0 39.5 20.0 40.5 54-55 33.679969878614415 38.0 33.0 40.0 20.0 41.0 56-57 33.55758464889385 38.0 33.0 40.0 18.5 41.0 58-59 33.29278946831555 37.0 33.0 40.0 17.5 41.0 60-61 32.9504263196107 37.0 32.5 40.0 14.5 41.0 62-63 32.62816109541039 36.0 32.0 39.5 13.0 41.0 64-65 32.276834134370176 36.0 31.5 39.0 9.5 41.0 66-67 31.890905981675157 35.0 31.0 39.0 7.5 41.0 68-69 31.47702134298178 35.0 31.0 38.5 7.0 40.0 70-71 31.026004596211425 35.0 30.5 38.0 2.0 40.0 72-73 30.656231586652986 35.0 30.0 37.0 2.0 39.5 74-75 30.240757474843843 35.0 30.0 37.0 2.0 39.0 76-77 29.796918258239877 34.0 29.0 36.0 2.0 39.0 78-79 29.359178322202823 34.0 29.0 36.0 2.0 38.5 80-81 28.90927222652242 34.0 29.0 35.0 2.0 37.0 82-83 28.446709748648438 34.0 27.5 35.0 2.0 37.0 84-85 28.104820021720997 34.0 27.0 35.0 2.0 36.0 86-87 27.77822199821192 34.0 27.0 35.0 2.0 36.0 88-89 27.354592311246318 33.5 26.0 35.0 2.0 35.5 90-91 26.92064814981489 33.0 25.0 35.0 2.0 35.0 92-93 26.565839228604517 33.0 25.0 35.0 2.0 35.0 94-95 26.10854439304208 33.0 24.0 35.0 2.0 35.0 96-97 25.51196695047972 32.5 21.5 35.0 2.0 35.0 98-99 24.799600981645156 32.0 10.0 35.0 2.0 35.0 100-101 22.507442142338547 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.550693143037357 1101 2 0.6947463201800232 1101 3 0.6529776493011923 1101 4 0.73674845298995 1101 5 0.6480885656473703 1101 6 0.6124233811192425 1101 7 0.6328359906527794 1101 8 0.5410754658806525 1101 9 0.9218600746699863 1101 10-11 0.8683501133931557 1101 12-13 0.9356924847523374 1101 14-15 1.2076246448161143 1101 16-17 1.1319102653858124 1101 18-19 1.1402631056686445 1101 20-21 1.0114430190434263 1101 22-23 1.0672722712561509 1101 24-25 1.0915163990833676 1101 26-27 0.9844610855090963 1101 28-29 1.1490915360429383 1101 30-31 1.0780775064812715 1101 32-33 0.9619027106984674 1101 34-35 0.9497958721461472 1101 36-37 1.085543952020629 1101 38-39 0.9646160625443088 1101 40-41 0.87793129280751 1101 42-43 1.0260564268905341 1101 44-45 0.9412405271459079 1101 46-47 1.1010636579389512 1101 48-49 1.1564243909379641 1101 50-51 1.109011617656023 1101 52-53 1.2174356106760058 1101 54-55 1.3054348885467775 1101 56-57 1.0866631719398683 1101 58-59 1.1835704103676434 1101 60-61 1.1870809192459078 1101 62-63 1.1015726880487833 1101 64-65 1.0441715309933741 1101 66-67 1.043736282657381 1101 68-69 1.1552391551248817 1101 70-71 1.1214551326555693 1101 72-73 0.974375895655637 1101 74-75 0.9856405848882197 1101 76-77 1.0754233677893943 1101 78-79 1.0990588452892034 1101 80-81 1.3090681585177002 1101 82-83 1.105365120297595 1101 84-85 1.1994848340462667 1101 86-87 1.0862178444244854 1101 88-89 1.1311866592496465 1101 90-91 1.0012174308319146 1101 92-93 1.0731541848947757 1101 94-95 1.2222733408519275 1101 96-97 1.1849849500029066 1101 98-99 1.2014367734486875 1101 100-101 1.5639246053201745 1102 1 0.4811671308758285 1102 2 0.6073158331990953 1102 3 0.4198383460771744 1102 4 0.460851063734367 1102 5 0.5978551231721028 1102 6 0.4881185113099775 1102 7 0.6352245532708025 1102 8 0.4537814063203385 1102 9 0.7255886577304622 1102 10-11 0.5643844064825672 1102 12-13 0.5469187290570403 1102 14-15 0.714882367041227 1102 16-17 0.6210512923594678 1102 18-19 0.6332879831005229 1102 20-21 0.8370649548402511 1102 22-23 0.5187842335588684 1102 24-25 0.5608776155828608 1102 26-27 0.4810471517218673 1102 28-29 0.6585147728809417 1102 30-31 0.5413417165881569 1102 32-33 0.5126537111662159 1102 34-35 0.2911383612752658 1102 36-37 0.2818565137378002 1102 38-39 0.2437757995642187 1102 40-41 0.558118349959777 1102 42-43 0.5640774497063958 1102 44-45 0.3748804973484283 1102 46-47 0.6015292435807282 1102 48-49 0.8423749652324446 1102 50-51 0.755548743238549 1102 52-53 0.8323031678022801 1102 54-55 0.9704416882626603 1102 56-57 0.8559925553745487 1102 58-59 0.9322884257168127 1102 60-61 0.725601093180039 1102 62-63 0.866822241952768 1102 64-65 0.8519431298361155 1102 66-67 0.9577707663120769 1102 68-69 0.8437558642356322 1102 70-71 0.6989090938149012 1102 72-73 0.9022805004059116 1102 74-75 0.753477928754613 1102 76-77 0.9420536538105644 1102 78-79 0.7448098890672448 1102 80-81 0.6253827125083085 1102 82-83 0.7002352327311137 1102 84-85 0.6052957759585027 1102 86-87 0.564588684470916 1102 88-89 0.7820115037656983 1102 90-91 0.9071756277378249 1102 92-93 0.6468942884929945 1102 94-95 0.9096672306358933 1102 96-97 0.47715076778784393 1102 98-99 0.591382072549564 1102 100-101 0.5444977737279153 1103 1 0.3562099728296708 1103 2 0.5246890272700213 1103 3 0.6024834979524201 1103 4 0.5401426600401606 1103 5 0.6924551606979037 1103 6 0.4372176836576358 1103 7 0.48094890542957813 1103 8 0.560316732690552 1103 9 0.8633946493497007 1103 10-11 0.7929190240321375 1103 12-13 0.6396649921128841 1103 14-15 0.7567897200130673 1103 16-17 0.908864789138498 1103 18-19 0.8333294699560625 1103 20-21 0.9254467589972748 1103 22-23 0.7655928728317036 1103 24-25 0.9594305790785924 1103 26-27 0.8098440329110161 1103 28-29 0.5460213920149286 1103 30-31 0.5633993812326068 1103 32-33 0.7384810507955422 1103 34-35 0.5900674166675159 1103 36-37 0.7557635748423053 1103 38-39 0.7146659949068521 1103 40-41 0.7154834134409143 1103 42-43 0.8386360003766455 1103 44-45 0.7667605398776658 1103 46-47 1.0740003174440034 1103 48-49 1.1568019671295744 1103 50-51 1.2133939284700688 1103 52-53 1.302106574310919 1103 54-55 1.3748824496194274 1103 56-57 1.4360536390362313 1103 58-59 1.2037542251205622 1103 60-61 1.3943181278080132 1103 62-63 1.3249688844361387 1103 64-65 1.3387302960128693 1103 66-67 1.202787965636606 1103 68-69 1.2783417253484828 1103 70-71 1.2914794025688323 1103 72-73 1.2603819034538404 1103 74-75 1.0964168242452672 1103 76-77 1.0323486482859323 1103 78-79 1.1221182468965871 1103 80-81 1.240806441321869 1103 82-83 1.0077011989161981 1103 84-85 1.1315302705068362 1103 86-87 1.2011052479416335 1103 88-89 1.2569861738513843 1103 90-91 1.119832454306092 1103 92-93 1.1553177879822236 1103 94-95 1.2878393072719128 1103 96-97 1.5580324155873733 1103 98-99 1.5056018486456288 1103 100-101 1.7182474349533408 1104 1 0.16391069536326697 1104 2 0.26168139677504954 1104 3 0.2389829239771366 1104 4 0.20950858616616586 1104 5 0.30493675972552126 1104 6 0.1619202294068458 1104 7 0.3404856736110915 1104 8 0.31280778542875254 1104 9 0.588029668594757 1104 10-11 0.5774730114722004 1104 12-13 0.36736976623673456 1104 14-15 0.49084738764761227 1104 16-17 0.32472001231359116 1104 18-19 0.4308458451333266 1104 20-21 0.4271403237214244 1104 22-23 0.15407015016305792 1104 24-25 0.2962811429249044 1104 26-27 -0.06904718189078807 1104 28-29 0.5301688699000024 1104 30-31 0.5440408830220562 1104 32-33 0.2697174610865858 1104 34-35 0.13170696018834605 1104 36-37 0.4092715767330475 1104 38-39 0.23210268880920637 1104 40-41 0.4860310533895955 1104 42-43 0.3425255270158516 1104 44-45 0.27702321797882234 1104 46-47 0.13845166459776692 1104 48-49 0.3890904403553179 1104 50-51 0.25053215522406447 1104 52-53 0.2668361306617939 1104 54-55 0.35924196072753745 1104 56-57 0.20348695661338212 1104 58-59 0.19567130257346577 1104 60-61 0.3369948784326411 1104 62-63 0.45865515629785136 1104 64-65 0.17902741263114308 1104 66-67 0.21520744028040895 1104 68-69 0.3966238510828326 1104 70-71 0.2726975807037526 1104 72-73 0.27763492526490197 1104 74-75 -0.19285996689525575 1104 76-77 0.2735155062776933 1104 78-79 0.34152536419012236 1104 80-81 0.37896836987882665 1104 82-83 0.4376329971470021 1104 84-85 0.5983153336441198 1104 86-87 0.6256254721296308 1104 88-89 0.5933410931572105 1104 90-91 0.6203105419554937 1104 92-93 0.6064285989755405 1104 94-95 0.5359816531426667 1104 96-97 0.6488082951200909 1104 98-99 1.062846767276195 1104 100-101 1.288589794329397 1105 1 0.33106196856659764 1105 2 0.3686385579882092 1105 3 0.4580086466442772 1105 4 0.29420186174539253 1105 5 0.44925441809811417 1105 6 0.5158149951713682 1105 7 0.4325781556100736 1105 8 0.5829563998958278 1105 9 0.7234854115458731 1105 10-11 0.6227289047348563 1105 12-13 0.525684175940718 1105 14-15 0.29285841049334493 1105 16-17 0.40945611859839914 1105 18-19 0.2235023994434684 1105 20-21 0.3148729631434577 1105 22-23 0.24077525671454225 1105 24-25 0.19052041302503397 1105 26-27 0.39170534552489755 1105 28-29 0.17006890464944036 1105 30-31 0.045885027741356055 1105 32-33 0.07056720612266076 1105 34-35 0.319008868175068 1105 36-37 0.3874323615855175 1105 38-39 0.19266853899870995 1105 40-41 -0.034280398571318926 1105 42-43 0.46268626338073915 1105 44-45 0.5241810370069686 1105 46-47 0.646722839532913 1105 48-49 0.7270457186336827 1105 50-51 0.8704981330975627 1105 52-53 1.0489381485537095 1105 54-55 0.9121358617966919 1105 56-57 0.8553925189482214 1105 58-59 0.8486419756620407 1105 60-61 0.7713903593891374 1105 62-63 0.7264929329801717 1105 64-65 0.9751084030710047 1105 66-67 0.9512102848857644 1105 68-69 0.9879773013790114 1105 70-71 1.0214268017505965 1105 72-73 1.030793083706186 1105 74-75 1.1152568558745166 1105 76-77 0.7059836054833077 1105 78-79 1.000677397032387 1105 80-81 0.7775125299525278 1105 82-83 0.9964274340040369 1105 84-85 0.8764922810270015 1105 86-87 1.1376253428293843 1105 88-89 1.0170006132916818 1105 90-91 1.170968356943007 1105 92-93 0.9013022483940922 1105 94-95 0.7243975635376039 1105 96-97 1.268009381242134 1105 98-99 1.2838814924727693 1105 100-101 1.25049551828954 1106 1 0.120893291295328 1106 2 0.08555432611034064 1106 3 0.3618545951870331 1106 4 0.1903745958117966 1106 5 0.30319111074250316 1106 6 0.16567887901891964 1106 7 0.21033784387739018 1106 8 0.28791262921151173 1106 9 0.6161644976993941 1106 10-11 0.24275399281001597 1106 12-13 0.40249505455713575 1106 14-15 0.7129248672030783 1106 16-17 0.5913246464087933 1106 18-19 0.6451648847642701 1106 20-21 0.5802594262586425 1106 22-23 0.4524372082465149 1106 24-25 0.21542299656298525 1106 26-27 0.14385384557827763 1106 28-29 0.471799714940893 1106 30-31 0.3716777041585644 1106 32-33 0.26878435141998125 1106 34-35 0.23590595368803946 1106 36-37 0.08898168367370118 1106 38-39 0.20215930445189656 1106 40-41 0.28834023770496486 1106 42-43 0.1410891671919856 1106 44-45 0.45785777448961795 1106 46-47 0.5467054913958336 1106 48-49 0.5866659278789044 1106 50-51 0.44741043159227445 1106 52-53 0.6661074663715851 1106 54-55 0.6888052863723289 1106 56-57 0.8221308022833327 1106 58-59 0.7220526194113788 1106 60-61 0.8661178601898172 1106 62-63 0.7345384651676383 1106 64-65 0.6408511702323096 1106 66-67 0.5727299816937013 1106 68-69 0.5785959626366193 1106 70-71 0.8099249868685661 1106 72-73 0.8324158017589234 1106 74-75 0.4013343086342047 1106 76-77 0.4858477562252723 1106 78-79 0.7410745359242412 1106 80-81 0.6957774493504125 1106 82-83 0.933305570013431 1106 84-85 0.8654441866531357 1106 86-87 1.0261208590031892 1106 88-89 1.1420619990074918 1106 90-91 1.037200875358721 1106 92-93 1.1659667621124647 1106 94-95 1.0388071930881502 1106 96-97 1.131883887487465 1106 98-99 1.4830527853093223 1106 100-101 1.8011867748284516 1107 1 -0.051966095978830396 1107 2 0.1414459207686285 1107 3 -0.05477934039819843 1107 4 0.12144293830983344 1107 5 0.2186074500983537 1107 6 0.06872286959237073 1107 7 0.0466104283235822 1107 8 0.3574530848281654 1107 9 0.40620770272688844 1107 10-11 0.3024358466984651 1107 12-13 0.39152686916828827 1107 14-15 0.3777029504709404 1107 16-17 0.2551207972336158 1107 18-19 0.5102179091161929 1107 20-21 0.5799648465257334 1107 22-23 0.4857345373902646 1107 24-25 0.39351608375859115 1107 26-27 0.4202285509985444 1107 28-29 0.5508354572818135 1107 30-31 0.2949298644099443 1107 32-33 0.16968772926759357 1107 34-35 0.3587064250156047 1107 36-37 0.10167807016231478 1107 38-39 0.11244995645503764 1107 40-41 -0.09588010793525115 1107 42-43 0.28178044763189547 1107 44-45 0.22839980119817938 1107 46-47 -0.00848893122788752 1107 48-49 -0.1597500187039671 1107 50-51 -0.06430402245328537 1107 52-53 0.16117816550748643 1107 54-55 0.1625778708185166 1107 56-57 0.08119403873266862 1107 58-59 0.41014177887720393 1107 60-61 0.47088023253859745 1107 62-63 0.39389628134988186 1107 64-65 0.4718115001616212 1107 66-67 0.5102987169647157 1107 68-69 0.31852133577094577 1107 70-71 0.29885860823541677 1107 72-73 0.1577889360872824 1107 74-75 0.19908568333962506 1107 76-77 0.19282929589534348 1107 78-79 0.24417744244427197 1107 80-81 0.23583047370233956 1107 82-83 0.5414654286151581 1107 84-85 0.42507694391943573 1107 86-87 0.3577685416426206 1107 88-89 0.2532953061559624 1107 90-91 0.4413249916195241 1107 92-93 0.4780347118375232 1107 94-95 0.5942470988226383 1107 96-97 0.44477666046467945 1107 98-99 0.7114404522378379 1107 100-101 1.0180956514977382 1108 1 0.1452353965584834 1108 2 0.14288515317801043 1108 3 0.1400500839088359 1108 4 0.2660324905486391 1108 5 0.18326629871242517 1108 6 0.2878593301467376 1108 7 -0.1291358403331344 1108 8 0.04740511041452322 1108 9 0.12659682852645915 1108 10-11 0.047265271005500153 1108 12-13 0.22204392673758377 1108 14-15 -0.010383403473618102 1108 16-17 0.39179457122082084 1108 18-19 0.0899487193507369 1108 20-21 0.1845357420481193 1108 22-23 0.20802931350966958 1108 24-25 0.41382525220208066 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-0.3081789789572369 2316 60-61 -0.03147147754566504 2316 62-63 0.12081978192816933 2316 64-65 -0.3685365749609275 2316 66-67 -0.4785760971565587 2316 68-69 -0.9738948122711335 2316 70-71 -1.3542889381654213 2316 72-73 -0.6875103975030683 2316 74-75 -0.2391419974446478 2316 76-77 -0.019815964885623316 2316 78-79 -1.0709957689893344 2316 80-81 -0.655982601144828 2316 82-83 -0.43106858030701645 2316 84-85 -0.7012847898471541 2316 86-87 -1.2908848702646303 2316 88-89 -1.1847781641309787 2316 90-91 -1.222898173000182 2316 92-93 -1.2916050965008594 2316 94-95 -1.5528173942103543 2316 96-97 -2.5336118398382332 2316 98-99 -3.8246695879670405 2316 100-101 -4.388466555915379 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1905.0 3 791.0 4 762.0 5 839.0 6 949.0 7 1144.0 8 1389.0 9 1871.0 10 2397.0 11 3080.0 12 3935.0 13 5041.0 14 6082.0 15 7701.0 16 9610.0 17 12031.0 18 9926.0 19 6641.0 20 6286.0 21 6808.0 22 7193.0 23 8066.0 24 9049.0 25 10297.0 26 12019.0 27 14039.0 28 16721.0 29 19875.0 30 24313.0 31 29431.0 32 36474.0 33 46289.0 34 60550.0 35 82128.0 36 111054.0 37 137324.0 38 103019.0 39 16259.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.3108531505468 18.299340960109483 14.616280326038677 21.773525563305043 2 15.820561941251595 19.987228607918265 40.32627065748663 23.86593879334351 3 19.143118468780983 25.534800759559918 29.90563116212187 25.416449609537228 4 12.512847605782623 16.526343595408484 37.13018106719178 33.83062773161712 5 13.239509001204322 37.699514788679124 33.60133424107835 15.45964196903821 6 31.225100438736575 35.981922228119004 17.4907485897753 15.302228743369122 7 28.246745059803484 33.89352446113224 20.295361132357762 17.564369346706517 8 27.23289914599284 32.99915835931694 19.666297273252276 20.101645221437945 9 27.505865007575885 16.388163850422497 20.382492141970403 35.72347900003122 10-11 25.111023723684962 26.56920633872367 26.478430148261772 21.841339789329595 12-13 26.757883905732317 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21.91211629492362 38-39 21.553343407223196 25.865992460185534 30.527727001585497 22.052937131005777 40-41 21.706472476956872 25.977093982835186 30.548516725280805 21.767916814927133 42-43 21.59199394673632 26.126181265484938 29.986396958006985 22.29542782977176 44-45 21.565710781475296 26.230968952082534 30.090432167473825 22.11288809896835 46-47 22.240869802620328 26.600023401146654 29.217111638470282 21.94199515776273 48-49 22.127062855785056 26.833401237638466 28.62073455457076 22.41880135200572 50-51 21.986890549771267 27.23781099542537 28.5691570301887 22.20614142461466 52-53 22.458514222623588 27.038136012745007 28.347679466677867 22.155670297953538 54-55 21.813346326897758 27.031874637706384 28.521433980651274 22.63334505474459 56-57 22.03921408591187 27.379524200183763 28.05389701306316 22.52736470084121 58-59 22.313150922505905 26.810971054108144 28.154598127083474 22.721279896302477 60-61 21.854244228890472 26.929364067777083 28.92785903058322 22.288532672749227 62-63 21.88392978228283 26.999341123833915 28.92112328185138 22.19560581203187 64-65 21.541050888340862 27.147708794604554 28.691159793350497 22.62008052370409 66-67 21.668805884439035 27.20694966747615 28.38216930483523 22.742075143249583 68-69 21.96820755826654 26.96878368224842 28.629401047105336 22.433607712379704 70-71 21.882896533101306 26.950539312020883 28.34731994179331 22.819244213084506 72-73 21.76695674714635 27.176818683399045 28.39424463367114 22.661979935783467 74-75 21.701152515197755 27.784875030677537 28.003773111659026 22.510199342465686 76-77 21.859384985583254 27.43435955750646 28.065395912905462 22.640859544004822 78-79 21.90946813202639 27.513478969207643 27.97546932615655 22.601583572609414 80-81 22.08209862540852 27.821105074094476 27.553880073404365 22.542916227092643 82-83 22.056140830939025 27.652221606142874 27.639559975324822 22.652077587593283 84-85 21.82964659791909 27.72160171494861 27.531358605860092 22.917393081272216 86-87 22.146941405975753 27.746337985995805 27.284112040736847 22.82260856729159 88-89 21.972296329899777 27.623561801002584 27.21527950295651 23.188862366141134 90-91 21.85036771412387 27.617922943249134 27.556477241087972 22.97523210153902 92-93 22.054690524130024 27.375088895357035 27.659494024131526 22.910726556381412 94-95 21.727963437371915 27.27890825797632 27.782088464901307 23.211039839750455 96-97 21.805421786158995 27.224867605802842 27.742742697691163 23.226967910347003 98-99 21.837006734619038 27.557981882968512 27.159642460809753 23.445368921602697 100-101 21.96055620386778 27.180129950584202 27.341907862826737 23.51740598272128 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 369.0 1 278.5 2 286.5 3 379.5 4 336.0 5 336.0 6 526.0 7 941.5 8 1435.0 9 1767.5 10 1636.5 11 1365.5 12 1373.0 13 1430.5 14 1465.5 15 1609.5 16 1752.0 17 1749.0 18 1833.5 19 2019.5 20 2218.0 21 2481.5 22 2837.0 23 3374.5 24 3956.5 25 4611.5 26 5552.0 27 6730.0 28 7851.0 29 9087.0 30 10587.5 31 12465.5 32 15077.0 33 16482.0 34 16555.5 35 17460.0 36 19565.0 37 21225.5 38 21786.0 39 21905.5 40 22393.0 41 23650.0 42 24271.5 43 25005.0 44 25971.5 45 27377.0 46 27922.0 47 28416.0 48 31182.0 49 31114.0 50 28615.0 51 27252.0 52 26660.0 53 27293.0 54 27528.0 55 25571.5 56 23039.5 57 21091.0 58 20328.5 59 18610.0 60 15316.5 61 13055.5 62 11408.0 63 8854.0 64 6254.0 65 4784.0 66 3627.0 67 2844.0 68 2174.0 69 1548.5 70 1204.5 71 916.5 72 720.0 73 657.0 74 561.0 75 464.0 76 354.0 77 221.0 78 142.0 79 105.0 80 76.5 81 66.0 82 49.5 83 29.0 84 19.5 85 9.0 86 3.5 87 3.5 88 5.0 89 5.0 90 2.5 91 3.0 92 3.0 93 2.0 94 1.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.031801462867291894 2 0.021961010206469497 3 0.021720999165961635 4 0.05460251171553891 5 0.05544255035731644 6 0.05388247859401533 7 0.058082671802902934 8 0.047762197061064805 9 0.046682147378779425 10-11 0.05730263592125238 12-13 0.04938227158449289 14-15 0.04506207285535135 16-17 0.051482368188936686 18-19 0.05556255587757037 20-21 0.012540576866535862 22-23 0.022621040567866124 24-25 0.007380339495616799 26-27 0.012660582386789793 28-29 0.007680353296251627 30-31 0.011580532704504407 32-33 0.006360292573458379 34-35 0.009480436100060604 36-37 0.012360568586154962 38-39 0.014160651389963938 40-41 0.0024601131652055995 42-43 0.0030601407664752577 44-45 0.0022201021246977364 46-47 3.0001380063482923E-4 48-49 0.0017400800436820094 50-51 3.600165607617951E-4 52-53 0.0033001518069831213 54-55 0.007440342255743765 56-57 0.01806083079821672 58-59 0.012300565826027997 60-61 0.011160513383615647 62-63 0.0069603201747280375 64-65 0.0 66-67 0.005580256691807823 68-69 0.016920778355804366 70-71 0.006900317414601071 72-73 0.0024601131652055995 74-75 0.0036601683677449162 76-77 0.0075003450158707295 78-79 0.006300289813331413 80-81 0.01224056306590103 82-83 0.008280380897521285 84-85 0.01806083079821672 86-87 0.00534024565129996 88-89 0.005760264972188721 90-91 0.0046802152899033354 92-93 0.01830084183872458 94-95 0.011340521663996544 96-97 0.009900455420949363 98-99 0.02538116753370655 100-101 0.0435620038521772 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 833295.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.277048706271664 #Duplication Level Percentage of deduplicated Percentage of total 1 82.0150808384842 49.43627022350119 2 10.597195423533087 12.775353293883661 3 3.1095735215946174 5.623077438506743 4 1.3372542245943828 3.2242295211417265 5 0.7057536697263718 2.1270374162363237 6 0.4569358474179481 1.6525646598271906 7 0.26663898431657895 1.1250547731248866 8 0.22614529511724213 1.0905096774780945 9 0.16131146680204667 0.8751041227176382 >10 1.0052798949505126 11.158186572150024 >50 0.07491631667018024 3.0586964455726053 >100 0.03914551951171969 4.511102805563961 >500 0.0037754561809247673 1.5567215312155986 >1k 7.94832880194688E-4 1.1256496845706085 >5k 1.98708220048672E-4 0.6604418345097185 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5514 0.6617104386801793 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4402 0.5282643001578072 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2685 0.32221482188180656 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1206 0.1447266574262416 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1105 0.13260609988059452 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1000 0.12000552025393167 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 861 0.10332475293863518 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.000276012696584E-5 0.0 14-15 0.0 0.0 0.0 1.2000552025393168E-4 0.0 16-17 0.0 0.0 0.0 1.2000552025393168E-4 0.0 18-19 0.0 0.0 0.0 1.2000552025393168E-4 0.0 20-21 0.0 0.0 0.0 1.2000552025393168E-4 0.0 22-23 0.0 0.0 0.0 1.800082803808975E-4 0.0 24-25 0.0 0.0 0.0 3.000138006348292E-4 0.0 26-27 0.0 0.0 0.0 7.800358816505558E-4 0.0 28-29 0.0 0.0 0.0 0.0021600993645707702 0.0 30-31 0.0 0.0 0.0 0.005700262212061755 0.0 32-33 0.0 0.0 0.0 0.009000414019044877 0.0 34-35 0.0 0.0 0.0 0.011760540984885305 0.0 36-37 0.0 0.0 0.0 0.015600717633011118 0.0 38-39 0.0 0.0 0.0 0.023281070929262744 0.0 40-41 0.0 0.0 0.0 0.037321716798972754 0.0 42-43 0.0 0.0 0.0 0.058502691123791695 0.0 44-45 0.0 0.0 0.0 0.08262380069483197 0.0 46-47 0.0 0.0 0.0 0.13548623236668886 0.0 48-49 0.0 0.0 0.0 0.18324842942775366 0.0 50-51 0.0 0.0 0.0 0.2214701876286309 0.0 52-53 0.0 0.0 0.0 0.26989241505109235 0.0 54-55 0.0 0.0 0.0 0.3159745348286021 0.0 56-57 0.0 0.0 0.0 0.405678661218416 0.0 58-59 0.0 0.0 0.0 0.5190238750982545 0.0 60-61 0.0 0.0 0.0 0.642809569240185 0.0 62-63 0.0 0.0 0.0 0.7688153655068133 0.0 64-65 0.0 0.0 0.0 0.9247025363166705 0.0 66-67 0.0 0.0 0.0 1.12247163369515 0.0 68-69 6.000276012696584E-5 0.0 0.0 1.34040165847629 0.0 70-71 1.2000552025393168E-4 0.0 0.0 1.531870466041438 0.0 72-73 1.2000552025393168E-4 0.0 0.0 1.6918978273000558 0.0 74-75 1.2000552025393168E-4 0.0 0.0 1.8573854397302276 0.0 76-77 1.2000552025393168E-4 0.0 0.0 2.0691351802182902 0.0 78-79 1.2000552025393168E-4 0.0 0.0 2.2901253457659054 0.0 80-81 1.2000552025393168E-4 0.0 0.0 2.5607977966986484 0.0 82-83 1.2000552025393168E-4 0.0 0.0 2.843290791376403 0.0 84-85 1.2000552025393168E-4 0.0 0.0 3.225688381665557 0.0 86-87 1.2000552025393168E-4 0.0 0.0 3.6722289225304365 0.0 88-89 1.2000552025393168E-4 0.0 0.0 4.2158539292807475 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6180 0.0 34.599075 1 GGTATCA 3925 0.0 33.654686 1 GTACATG 5390 0.0 28.033596 1 TAAGGTG 395 0.0 27.670902 5 ATCAACG 7625 0.0 27.60605 3 TCAACGC 7690 0.0 27.437796 4 CAACGCA 7715 0.0 27.410482 5 TACATGG 5430 0.0 27.127035 2 TATCAAC 7860 0.0 26.901587 2 AACGCAG 7915 0.0 26.7779 6 ACATGGG 5365 0.0 25.95006 3 GTAAGGT 315 0.0 25.646667 4 GTATAGG 260 0.0 25.585571 1 CATGGGG 2030 0.0 23.877934 4 ACGCAGA 8815 0.0 23.828272 7 CATGGGT 600 0.0 23.760883 4 CGCAGAG 8875 0.0 23.66718 8 GAGTACT 4580 0.0 23.139717 12-13 GGTAAGG 350 0.0 23.07923 3 AGTCCGT 105 0.0021898786 22.629414 6 >>END_MODULE