FastQCFastQC Report
Thu 26 May 2016
SRR936737_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936737_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833295
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64210.7705554455504953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56760.6811513329613162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35850.43021979011034506No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14760.17712814789480316No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14160.16992781667956725No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC13300.15960734193772913No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12690.1522870052022393No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA11080.1329661164413563No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10270.12324566930078784No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA9870.11844544849063057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47050.037.980061
GTATCAA72900.033.052771
AGGTAAG4650.028.6020952
GTAAGGT5350.026.6322654
ATCAACG90850.026.4017773
TCAACGC92350.026.0228164
TATCAAC93350.025.8997922
CAACGCA93500.025.7027475
TAAGGTG5950.025.5431065
AACGCAG95350.025.1542476
GTACATG50800.024.8854431
GGTGTGC8800.024.8265198
GAGTACT61050.024.77938512-13
TACATGG51900.024.436372
ACATGGG50950.023.7718353
AAGGTAA5600.023.762791
GGTAAGG6000.023.7485313
GTACTTT63900.023.5255414-15
ACGCAGA107200.022.2850637
CGCAGAG107750.022.1272328
Produced by FastQC (version 0.11.5)