##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936737_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 833295 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.764929586760992 31.0 31.0 34.0 30.0 34.0 2 31.895808807205132 31.0 31.0 34.0 30.0 34.0 3 31.973254369701007 31.0 31.0 34.0 30.0 34.0 4 35.76618724461325 37.0 35.0 37.0 35.0 37.0 5 35.52588339063597 37.0 35.0 37.0 33.0 37.0 6 35.641359902555514 37.0 35.0 37.0 33.0 37.0 7 35.57779777869782 37.0 35.0 37.0 33.0 37.0 8 35.551793782514 37.0 35.0 37.0 33.0 37.0 9 37.15434029965378 39.0 37.0 39.0 33.0 39.0 10-11 37.01925668580755 39.0 37.0 39.0 33.0 39.0 12-13 36.90344295837609 39.0 37.0 39.0 33.0 39.0 14-15 37.93304952027793 40.0 37.0 41.0 33.0 41.0 16-17 37.86722949255666 40.0 37.0 41.0 33.0 41.0 18-19 38.0515129695966 40.0 37.0 41.0 33.0 41.0 20-21 38.081755560755795 40.0 37.0 41.0 33.0 41.0 22-23 37.9866763871138 40.0 37.0 41.0 33.0 41.0 24-25 37.83003738171956 40.0 37.0 41.0 32.5 41.0 26-27 37.58026989241505 39.0 36.5 41.0 32.0 41.0 28-29 37.45471891707018 39.0 36.0 41.0 32.0 41.0 30-31 37.34043706010476 39.0 36.0 41.0 32.0 41.0 32-33 37.11610894101129 39.0 35.5 41.0 31.0 41.0 34-35 36.8939025195159 39.0 35.0 41.0 31.0 41.0 36-37 36.597397080265694 39.0 35.0 41.0 30.5 41.0 38-39 36.36194684955508 39.0 35.0 40.0 30.0 41.0 40-41 36.054572510335475 38.5 35.0 40.0 30.0 41.0 42-43 35.72817909623843 38.0 35.0 40.0 29.0 41.0 44-45 35.35445130476002 38.0 35.0 40.0 27.5 41.0 46-47 34.823033259529936 38.0 34.0 40.0 25.0 41.0 48-49 34.31015726723429 38.0 33.5 40.0 23.0 41.0 50-51 33.92067095086374 38.0 33.0 40.0 22.0 41.0 52-53 33.59799710786696 38.0 33.0 40.0 20.5 41.0 54-55 33.30759034915606 37.0 33.0 40.0 19.5 41.0 56-57 32.96375773285571 37.0 32.0 40.0 17.5 41.0 58-59 32.5887050804337 36.5 31.5 39.5 14.5 41.0 60-61 32.22770687451623 36.0 31.0 39.0 12.5 40.0 62-63 31.797300475821885 35.5 31.0 39.0 8.5 40.0 64-65 31.374590631169035 35.0 30.5 38.5 7.5 40.0 66-67 30.90612928194697 35.0 30.0 38.0 6.5 40.0 68-69 30.60706472497735 35.0 29.5 37.5 2.0 40.0 70-71 30.156147582788808 34.0 29.0 37.0 2.0 39.0 72-73 29.711775541674918 34.0 29.0 36.5 2.0 39.0 74-75 29.1775511673537 34.0 28.0 36.0 2.0 39.0 76-77 27.793079881674558 32.0 26.5 34.5 2.0 36.5 78-79 28.56173863997744 33.5 28.0 35.0 2.0 37.0 80-81 28.38030289393312 34.0 27.5 35.0 2.0 37.0 82-83 28.024494926766632 33.0 27.0 35.0 2.0 36.0 84-85 27.58041209895655 33.0 26.0 35.0 2.0 36.0 86-87 27.19702806329091 33.0 26.0 35.0 2.0 35.5 88-89 26.76715928932731 33.0 25.0 35.0 2.0 35.0 90-91 26.26057758656898 32.0 24.5 34.5 2.0 35.0 92-93 25.74800100804637 32.0 23.5 34.0 2.0 35.0 94-95 25.12015252701624 32.0 19.5 34.0 2.0 35.0 96-97 24.334737997947904 31.0 12.5 34.0 2.0 35.0 98-99 23.365017790818378 31.0 2.0 34.0 2.0 35.0 100-101 20.385461931248837 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4172257031465456 1101 2 0.5505347247866439 1101 3 0.5134590511419006 1101 4 0.40856073610214594 1101 5 0.28819365055774426 1101 6 0.5008344618310829 1101 7 0.5154304156747784 1101 8 0.3743878077401632 1101 9 0.5142095601297356 1101 10-11 0.47657835523675374 1101 12-13 0.4207848486155825 1101 14-15 0.6620098190029466 1101 16-17 0.8726413611450923 1101 18-19 0.6923032930595312 1101 20-21 0.5997025344946749 1101 22-23 0.6338685217028157 1101 24-25 0.6258405730367187 1101 26-27 0.5830524678883933 1101 28-29 0.5671661037651461 1101 30-31 0.7176619643326916 1101 32-33 0.820299572306169 1101 34-35 0.7862995087300959 1101 36-37 0.7676623068260682 1101 38-39 0.8427748163789843 1101 40-41 0.9218733622315014 1101 42-43 1.0604566869494008 1101 44-45 1.0300860881006528 1101 46-47 1.20892323377619 1101 48-49 1.2561130024087035 1101 50-51 1.162478352499896 1101 52-53 1.1773752132433515 1101 54-55 1.3045834748687284 1101 56-57 1.4584361571220157 1101 58-59 1.379491760615636 1101 60-61 1.383844950427207 1101 62-63 1.4639770384274193 1101 64-65 1.3947872109467845 1101 66-67 1.3179927665423499 1101 68-69 1.29380042014159 1101 70-71 1.3820229526107113 1101 72-73 1.3113431570474106 1101 74-75 1.4996790576273504 1101 76-77 1.4137909131177864 1101 78-79 1.3684922150751895 1101 80-81 1.4729391094087418 1101 82-83 1.7320503951683541 1101 84-85 1.7789719453997677 1101 86-87 1.5671966581388794 1101 88-89 1.5482987912284436 1101 90-91 1.8217392811309345 1101 92-93 1.563535517513344 1101 94-95 1.4061813052993521 1101 96-97 1.2166645264665057 1101 98-99 1.713847879838525 1101 100-101 2.076302934894194 1102 1 0.3705826838994497 1102 2 0.40946444084436706 1102 3 0.3600410028769119 1102 4 0.2753144564562504 1102 5 0.12980791177077577 1102 6 0.2590850725062026 1102 7 0.30922578320591754 1102 8 0.33720159080757384 1102 9 0.385114571996759 1102 10-11 0.4743836061765876 1102 12-13 0.42454309922625555 1102 14-15 0.5648117783605713 1102 16-17 0.6026861439872846 1102 18-19 0.45164207482763175 1102 20-21 0.3534391299592201 1102 22-23 0.5282845257220075 1102 24-25 0.5125657519082196 1102 26-27 0.3126262513022837 1102 28-29 0.38201352160552204 1102 30-31 0.5924096818802838 1102 32-33 0.6978732864236008 1102 34-35 0.6571557965183246 1102 36-37 0.8323072490501389 1102 38-39 0.7540320275426424 1102 40-41 0.7850063421528475 1102 42-43 0.8748764156537234 1102 44-45 0.8261660735484 1102 46-47 0.8418663413762886 1102 48-49 0.8896407989542752 1102 50-51 0.9534823716950172 1102 52-53 1.025189342793098 1102 54-55 1.042034317687019 1102 56-57 0.9847114286775493 1102 58-59 0.732002891360743 1102 60-61 0.80452323291426 1102 62-63 0.6804786928805875 1102 64-65 0.9346056108913494 1102 66-67 0.8722020938468944 1102 68-69 0.8775148840467963 1102 70-71 0.9697143841024882 1102 72-73 0.9728093403712172 1102 74-75 0.7830810377739077 1102 76-77 0.7863806953536425 1102 78-79 0.6894333910362462 1102 80-81 1.101555518812276 1102 82-83 0.9107454127523305 1102 84-85 0.8852229198813433 1102 86-87 1.1132690642854755 1102 88-89 1.0952590583663273 1102 90-91 1.2530145093761753 1102 92-93 1.1329684125465214 1102 94-95 1.1996020388024604 1102 96-97 1.36466445717004 1102 98-99 1.3133673521459208 1102 100-101 1.435338761167948 1103 1 0.2167327454000656 1103 2 0.308734772530741 1103 3 0.3609095166467995 1103 4 0.1447034113627481 1103 5 0.13088323438841343 1103 6 0.2409617794088632 1103 7 0.23968839291676147 1103 8 0.26627449509593504 1103 9 0.24829008849768996 1103 10-11 0.3332778459664425 1103 12-13 0.3038522075715804 1103 14-15 0.43182660105116355 1103 16-17 0.5655843865290322 1103 18-19 0.2864174412253675 1103 20-21 0.2879453529875562 1103 22-23 0.22733116195107783 1103 24-25 0.20957953221552117 1103 26-27 0.5586041110808821 1103 28-29 0.5567027871422425 1103 30-31 0.5423148249995151 1103 32-33 0.5548374492855643 1103 34-35 0.4710784933724099 1103 36-37 0.6392816272684954 1103 38-39 0.848700653889928 1103 40-41 0.7981664313808565 1103 42-43 0.8102736679313338 1103 44-45 0.807118394936019 1103 46-47 0.97862682254042 1103 48-49 0.9277740989313443 1103 50-51 1.138593177040363 1103 52-53 0.9736245169753488 1103 54-55 0.9424910900513481 1103 56-57 0.8390550399102708 1103 58-59 1.1185002749786506 1103 60-61 1.0779018056830374 1103 62-63 0.8219801417933787 1103 64-65 0.9450352812897478 1103 66-67 1.1858637779213588 1103 68-69 1.0629609198630838 1103 70-71 0.9872272575363077 1103 72-73 0.8150856970330871 1103 74-75 1.0593649412909123 1103 76-77 1.0196387686123387 1103 78-79 0.9837223813837745 1103 80-81 1.1087786096872492 1103 82-83 1.1382199264805202 1103 84-85 1.1832350949183486 1103 86-87 0.9365121272245815 1103 88-89 0.9522000803189208 1103 90-91 1.0197754074936292 1103 92-93 1.0369002823736935 1103 94-95 1.2507826421339203 1103 96-97 1.0657637546036902 1103 98-99 1.1222048862839742 1103 100-101 1.4445726094386373 1104 1 0.14725067368691924 1104 2 0.29472977556942936 1104 3 0.13101418272823295 1104 4 0.17687573900134623 1104 5 0.08003847782146067 1104 6 0.14885832880450067 1104 7 0.02126716258385386 1104 8 0.06341947450034979 1104 9 0.07678087112986987 1104 10-11 0.1736073753067089 1104 12-13 0.13480365601509448 1104 14-15 0.14373152373331521 1104 16-17 0.23245048147129665 1104 18-19 0.05382644926101676 1104 20-21 0.09148914392549301 1104 22-23 0.06648032799875381 1104 24-25 0.16245818708755877 1104 26-27 0.17908219873028486 1104 28-29 0.27431099836515216 1104 30-31 0.2680559283825943 1104 32-33 0.21698209105746002 1104 34-35 0.21898449647863316 1104 36-37 0.38512672146796945 1104 38-39 0.38071226844864015 1104 40-41 0.26416008388947176 1104 42-43 0.472323772597413 1104 44-45 0.5949987379706556 1104 46-47 0.518401284016548 1104 48-49 0.461477927684129 1104 50-51 0.23434440939905699 1104 52-53 0.13070602146586197 1104 54-55 0.08168212253159624 1104 56-57 0.37219029549809335 1104 58-59 0.27037143440872313 1104 60-61 0.17505218720778615 1104 62-63 0.15548004050362252 1104 64-65 0.3548023148504207 1104 66-67 0.23260427356590085 1104 68-69 0.3737192425047198 1104 70-71 0.3137395135968113 1104 72-73 0.3160756355839709 1104 74-75 0.43319031775117267 1104 76-77 0.3004760972638323 1104 78-79 0.474685984598036 1104 80-81 0.5152314424241027 1104 82-83 0.3478802681646336 1104 84-85 0.3331304288369097 1104 86-87 0.524789526103639 1104 88-89 0.3604396643556562 1104 90-91 0.23131973864360944 1104 92-93 0.4327474023682889 1104 94-95 0.5409534841894228 1104 96-97 0.6480610062749754 1104 98-99 0.9433460842041583 1104 100-101 1.3082338964938032 1105 1 0.04103294823982395 1105 2 0.16151092682647317 1105 3 0.05719101697340179 1105 4 0.12332685138750321 1105 5 0.12810261074211127 1105 6 0.04284990812615774 1105 7 0.1583634023711582 1105 8 0.10851535871198337 1105 9 0.1306829100330802 1105 10-11 0.0563971088673938 1105 12-13 -0.06949396081979842 1105 14-15 0.15252313214752888 1105 16-17 0.25163863756901605 1105 18-19 -0.019238693817783314 1105 20-21 0.11126642425650601 1105 22-23 0.15579164257816558 1105 24-25 0.04360297911619426 1105 26-27 0.08795502385978438 1105 28-29 0.10834888333641857 1105 30-31 0.23070310842510366 1105 32-33 0.3245283765039062 1105 34-35 0.35371575400431254 1105 36-37 0.37937892159241926 1105 38-39 0.5008160318938479 1105 40-41 0.532162718381926 1105 42-43 0.6232846350300392 1105 44-45 0.4609503473546184 1105 46-47 0.5179265096858785 1105 48-49 0.3612051849835254 1105 50-51 0.6553253941820429 1105 52-53 0.725300867234921 1105 54-55 0.6292735014378792 1105 56-57 0.466790798152779 1105 58-59 0.6829252758381372 1105 60-61 0.5165805968956043 1105 62-63 0.6589210432270072 1105 64-65 0.5280335279291393 1105 66-67 0.8028143056485249 1105 68-69 0.9490633023707318 1105 70-71 0.7837879203753779 1105 72-73 0.7736696004951185 1105 74-75 0.7017873292993002 1105 76-77 0.4040084182840147 1105 78-79 0.614871692072402 1105 80-81 0.7722908316420742 1105 82-83 0.8913409083624622 1105 84-85 0.8649657649771356 1105 86-87 0.8547487965270193 1105 88-89 1.0620244004619401 1105 90-91 1.039675362800125 1105 92-93 1.0173073370716672 1105 94-95 0.8530180050108029 1105 96-97 1.0493241080261804 1105 98-99 1.2497323040307364 1105 100-101 1.241823271246318 1106 1 0.11883800855796167 1106 2 0.05429677074661754 1106 3 -0.007426790222069712 1106 4 0.010457202620550277 1106 5 0.008010799062809326 1106 6 -0.09116578526553099 1106 7 -0.005226861320522858 1106 8 -0.15332407760701017 1106 9 -0.18696414879045165 1106 10-11 0.18425688254092876 1106 12-13 -0.08760408145607812 1106 14-15 0.1152900265641037 1106 16-17 0.21309107698398577 1106 18-19 0.10624571954828355 1106 20-21 0.08722724415614636 1106 22-23 0.13217282118390727 1106 24-25 -0.10761939731168724 1106 26-27 0.08149884792298678 1106 28-29 0.07718896020201527 1106 30-31 0.22914577950442094 1106 32-33 0.1993735777422998 1106 34-35 0.2176529804375349 1106 36-37 0.009993045644947074 1106 38-39 0.27099324978645 1106 40-41 0.14789881318282738 1106 42-43 0.26055243688762175 1106 44-45 0.1614828017156782 1106 46-47 0.009686144574317268 1106 48-49 0.31918132462269 1106 50-51 -0.009537955876680826 1106 52-53 -0.1789133956027058 1106 54-55 0.11252454232610631 1106 56-57 -0.04249045518339756 1106 58-59 -4.8723617210555403E-4 1106 60-61 0.2030690670568127 1106 62-63 0.048641604772857505 1106 64-65 -0.008468286952894033 1106 66-67 0.10990035240842744 1106 68-69 -0.10989367424931373 1106 70-71 -0.2897308156126641 1106 72-73 -0.2781212610400594 1106 74-75 -0.014167618923181635 1106 76-77 -0.2840253705491236 1106 78-79 -0.15342525465547396 1106 80-81 -0.23718258567403439 1106 82-83 -0.11766544372465049 1106 84-85 -0.4685601771601533 1106 86-87 -0.2152162403044109 1106 88-89 -0.2507838625891985 1106 90-91 -0.16695296003536342 1106 92-93 -0.021407032523722336 1106 94-95 0.25033438165220545 1106 96-97 0.03786232730663741 1106 98-99 0.552441282358906 1106 100-101 0.44445218124952035 1107 1 -0.1516254635551597 1107 2 0.06413573382595672 1107 3 0.030770381812494918 1107 4 -0.10890650515635514 1107 5 -0.11964041853342167 1107 6 0.04879886516651766 1107 7 -0.010450741917537698 1107 8 -0.08792737690001218 1107 9 0.08318510494089537 1107 10-11 0.15158799016072777 1107 12-13 0.0397034597850876 1107 14-15 0.12093613811162385 1107 16-17 0.0628750518465111 1107 18-19 -0.03777431187354807 1107 20-21 0.062030857667544126 1107 22-23 -0.162279260512868 1107 24-25 -0.0490569464083066 1107 26-27 0.13890261854356822 1107 28-29 0.09892894449109946 1107 30-31 0.17145724847535604 1107 32-33 0.030893609164138525 1107 34-35 0.16407874634484187 1107 36-37 0.26880097965909044 1107 38-39 0.23104823800326102 1107 40-41 0.3617636992786615 1107 42-43 0.133951887005459 1107 44-45 0.46316190619328523 1107 46-47 0.6236786818877533 1107 48-49 0.6152241368772025 1107 50-51 0.6553677786087917 1107 52-53 0.38524607022604584 1107 54-55 0.515362327086514 1107 56-57 0.5322247844081218 1107 58-59 0.6047562830737689 1107 60-61 0.8094221699633302 1107 62-63 0.9849829244900583 1107 64-65 0.9069578717273892 1107 66-67 0.7451124498161228 1107 68-69 0.5041675983351297 1107 70-71 0.8938905512372948 1107 72-73 1.1934616140581333 1107 74-75 0.9275055939597721 1107 76-77 0.20598837650507917 1107 78-79 0.5190904562636156 1107 80-81 0.5092137222599789 1107 82-83 0.672426857924993 1107 84-85 0.5259115431854866 1107 86-87 0.7938862734426451 1107 88-89 0.43847379648385854 1107 90-91 0.31789582236840985 1107 92-93 0.3965132345619047 1107 94-95 0.6866364240716862 1107 96-97 0.5584730892862098 1107 98-99 0.9066243145662938 1107 100-101 0.8121466038732414 1108 1 -0.08576725459993284 1108 2 0.09771782337819701 1108 3 0.0663514052666514 1108 4 0.04072036051528727 1108 5 -0.05618093026049564 1108 6 0.0998116583434907 1108 7 -0.03736971846338122 1108 8 0.03304810284411985 1108 9 0.1916072797809818 1108 10-11 0.07626816073641862 1108 12-13 0.09303501628828315 1108 14-15 -0.10848816892461599 1108 16-17 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-0.8124014055416566 2316 100-101 -1.2233328255479208 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 27.0 5 41.0 6 94.0 7 251.0 8 596.0 9 1030.0 10 1701.0 11 2680.0 12 3562.0 13 4877.0 14 6522.0 15 8345.0 16 10924.0 17 13886.0 18 11859.0 19 7563.0 20 6771.0 21 6898.0 22 6978.0 23 7919.0 24 8913.0 25 10622.0 26 12590.0 27 15441.0 28 19094.0 29 22785.0 30 27328.0 31 33018.0 32 40928.0 33 50718.0 34 65382.0 35 86030.0 36 115544.0 37 139433.0 38 78804.0 39 4134.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.77539064375786 19.54335257339877 15.344592453156364 21.336664329687004 2 16.156019278589053 21.145722323031578 39.012140326622315 23.686118071757058 3 19.345880641758466 25.67573892349806 30.47392631226501 24.504454122478467 4 13.047495841803732 17.4565039469863 36.436119784853844 33.05988042635612 5 13.143219219262464 38.44860546581078 33.10232104347951 15.305854271447242 6 31.11515129695966 36.9675805087034 16.565442010332475 15.351826184004464 7 28.007128327903086 34.44302437912144 19.955957974066806 17.59388931890867 8 27.583508841406708 33.54106288889289 19.00167407700754 19.873754192692864 9 27.4537734852776 16.39385182553931 20.130375092405025 36.021999596778066 10-11 25.392780424582078 26.987963374095454 25.95836983523143 21.660886366091038 12-13 26.987310995517117 24.737168336472532 27.07635664101104 21.199164026999313 14-15 23.273131372044283 25.632786637360738 26.671454981737796 24.422627008857184 16-17 21.413188858613307 28.972129732026115 27.450416820928115 22.164264588432463 18-19 21.42068535152617 26.04761819043676 31.570932262884092 20.96076419515298 20-21 22.357568448148616 25.008670398838344 31.84328479110039 20.79047636191265 22-23 21.851259148319023 25.094460875466456 32.01991163850392 21.034368337710603 24-25 21.846479527363453 25.407754628379553 32.004073046011975 20.741692798245015 26-27 21.271252650768258 25.72670354239849 31.888067072387727 21.113976734445526 28-29 21.050866130770505 26.075432705347517 31.8362764417711 21.037424722110877 30-31 21.273204901576054 25.61968742912072 31.81980191603573 21.287305753267496 32-33 21.02349230883698 25.78159689137327 31.589031002263308 21.60587979752644 34-35 21.685890246471075 25.709730245798983 31.415595972979578 21.188783534750364 36-37 22.106875231415778 25.38525288155773 31.260828157921427 21.24704372910507 38-39 21.621039379226456 25.72518493048342 31.19019800928568 21.46357768100444 40-41 21.560625834420893 25.874263084469646 31.14417292951112 21.42093815159834 42-43 21.632131891579228 25.999396368973365 30.639554873120478 21.72891686632693 44-45 21.685682826652425 26.070570304788593 30.524101970220187 21.7196448983388 46-47 22.29913913988392 26.680663318910664 29.523775807952653 21.496421733252767 48-49 22.519558040487752 26.798565683694374 28.729948433256645 21.951927842561226 50-51 22.219362070062125 27.100081724347735 28.839334252582237 21.8412219530079 52-53 22.653491178371333 27.1488224927379 28.515255360092745 21.682430968798023 54-55 21.990039302751192 27.254747832348265 28.637124598721908 22.11808826617863 56-57 22.310928378429463 27.362018924113336 28.363128401111037 21.963924296346164 58-59 22.46447164091696 26.775457890403924 28.34678222633983 22.41328824233929 60-61 22.052954501132596 26.95783141567033 29.093172919698173 21.896041163498897 62-63 22.04889072374785 27.162146592962944 29.05306476118697 21.735897922102236 64-65 21.84210921114615 27.175446545107583 28.713067951668414 22.269376292077855 66-67 21.998480653535 27.236385587570645 28.45270284055383 22.312430918340528 68-69 22.221148786053703 26.941140961304093 28.698289296114567 22.139420956527637 70-71 22.16006595511308 27.006554709382286 28.40918091333911 22.424198422165524 72-73 22.227969294632203 27.11237413361383 28.268355745915663 22.391300825838304 74-75 21.974278441392602 27.790132628286752 27.851760196152085 22.383828734168556 76-77 22.171645136069095 27.37661134456177 28.051601428826018 22.400142090543117 78-79 22.225789185660247 27.536651049367304 27.858397818698517 22.37916194627393 80-81 22.28270881526086 27.921703465677762 27.425269354359628 22.370318364701753 82-83 22.34261512834176 27.760775955182392 27.455823264253926 22.44078565222192 84-85 21.960042101372135 27.806048051731402 27.472465486596086 22.761444360300374 86-87 22.134626428073805 27.771973839674096 27.100923916272073 22.992475815980026 88-89 21.786071129594124 27.762985366614757 26.992819829632232 23.458123674158887 90-91 21.882784599403127 27.755256845646564 27.283387473587496 23.07857108136281 92-93 21.884145020638456 27.511222292293837 27.57194657942851 23.0326861076392 94-95 21.474612457291396 27.42627477759123 27.57736752981952 23.521745235297857 96-97 21.689408122119815 27.338229646697386 27.58028513824885 23.392077092933945 98-99 21.40357025202026 27.570412837017617 27.091382922100482 23.934633988861638 100-101 21.248211033535057 27.43802000042005 27.184069272381954 24.129699693662936 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 650.0 1 462.5 2 394.5 3 513.0 4 426.5 5 386.0 6 589.0 7 1098.5 8 1697.0 9 2046.5 10 1856.0 11 1529.5 12 1501.5 13 1536.5 14 1672.5 15 1798.5 16 1911.0 17 2061.5 18 2203.5 19 2370.5 20 2546.5 21 2812.0 22 3176.5 23 3673.5 24 4257.0 25 4827.0 26 5607.5 27 6731.0 28 7896.0 29 9276.0 30 10901.5 31 12807.5 32 15125.0 33 16352.5 34 16509.5 35 17177.5 36 19113.0 37 21078.5 38 21914.0 39 22043.0 40 22210.0 41 23182.5 42 23894.5 43 24715.5 44 25864.5 45 27218.5 46 27535.5 47 27971.0 48 30953.0 49 30885.5 50 28322.0 51 26942.0 52 26257.5 53 26587.5 54 26871.5 55 25451.0 56 22935.5 57 21261.5 58 20699.0 59 18815.5 60 15325.5 61 12793.0 62 11243.5 63 8790.5 64 6108.5 65 4647.5 66 3445.0 67 2734.5 68 2091.0 69 1476.0 70 1197.0 71 902.0 72 769.5 73 702.0 74 588.5 75 487.5 76 356.0 77 236.0 78 160.5 79 119.5 80 100.0 81 89.0 82 64.5 83 39.5 84 23.5 85 15.5 86 7.5 87 2.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.037321716798972754 2 0.0046802152899033354 3 0.002640121445586497 4 1.2000552025393168E-4 5 7.200331215235902E-4 6 0.0 7 0.0 8 0.0 9 8.400386417775218E-4 10-11 6.000276012696585E-4 12-13 0.0017400800436820094 14-15 0.0019800910841898728 16-17 0.0028201297259673945 18-19 0.0 20-21 0.0 22-23 0.0016200745234280778 24-25 0.0014400662430471803 26-27 0.005520253931680858 28-29 0.005880270492442653 30-31 0.0014400662430471803 32-33 1.2000552025393168E-4 34-35 0.008820405738663979 36-37 0.013020598947551587 38-39 0.009180422299425773 40-41 9.000414019044876E-4 42-43 5.400248411426926E-4 44-45 0.001500069003174146 46-47 6.000276012696584E-5 48-49 7.200331215235902E-4 50-51 7.200331215235902E-4 52-53 0.0036601683677449162 54-55 0.002400110405078634 56-57 0.0023401076449516677 58-59 0.002040093844316839 60-61 0.0039001794082527794 62-63 0.005880270492442653 64-65 0.01110051062348868 66-67 0.00504023185066513 68-69 0.004980229090538165 70-71 1.2000552025393168E-4 72-73 0.0024601131652055995 74-75 0.007680353296251627 76-77 0.0028201297259673945 78-79 0.0037201711278718823 80-81 0.006300289813331413 82-83 0.006120281532950515 84-85 0.008160375377267355 86-87 0.005820267732315687 88-89 0.011700538224758339 90-91 0.014340659670344834 92-93 0.0024601131652055995 94-95 0.0038401766481258137 96-97 0.0018000828038089753 98-99 0.005820267732315687 100-101 0.0075003450158707295 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 833295.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.326776217370835 #Duplication Level Percentage of deduplicated Percentage of total 1 80.5046661744301 46.150729822356965 2 11.264858954567252 12.915560968174455 3 3.4777992742279746 5.981130621678054 4 1.5236255605983335 3.4937816620594737 5 0.7617989071251807 2.1835737735701453 6 0.48558386504798606 1.6702174539823134 7 0.335625423646227 1.3468226487959274 8 0.24381883340773813 1.1181878160276657 9 0.18204012959779053 0.9392196394830349 >10 1.0828216552153749 11.531681758997236 >50 0.09010473619620996 3.553521642597955 >100 0.04179573646114071 4.883415349406991 >500 0.0035704900437329877 1.425809426266398 >1k 0.0014702017827135832 1.3498352877784654 >5k 4.200576522038809E-4 1.4565121288249125 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6421 0.7705554455504953 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5676 0.6811513329613162 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3585 0.43021979011034506 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1476 0.17712814789480316 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1416 0.16992781667956725 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1330 0.15960734193772913 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1269 0.1522870052022393 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1108 0.1329661164413563 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1027 0.12324566930078784 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 987 0.11844544849063057 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.000276012696584E-5 0.0 14-15 0.0 0.0 0.0 1.2000552025393168E-4 0.0 16-17 0.0 0.0 0.0 1.2000552025393168E-4 0.0 18-19 0.0 0.0 0.0 1.2000552025393168E-4 0.0 20-21 0.0 0.0 0.0 1.2000552025393168E-4 0.0 22-23 0.0 0.0 0.0 4.2001932088876087E-4 0.0 24-25 0.0 0.0 0.0 4.800220810157267E-4 0.0 26-27 0.0 0.0 0.0 9.600441620314533E-4 0.0 28-29 0.0 0.0 0.0 0.0023401076449516677 0.0 30-31 0.0 0.0 0.0 0.00606027877282355 0.0 32-33 0.0 0.0 0.0 0.009420433339933636 0.0 34-35 0.0 0.0 0.0 0.012420571346281929 0.0 36-37 0.0 0.0 0.0 0.016380753514661672 0.0 38-39 0.0 0.0 0.0 0.02448112613180206 0.0 40-41 0.0 0.0 0.0 0.03894179132240083 0.0 42-43 0.0 0.0 0.0 0.060722793248489434 0.0 44-45 0.0 0.0 0.0 0.08490390557965666 0.0 46-47 0.0 0.0 0.0 0.13812635381227537 0.0 48-49 0.0 0.0 0.0 0.18660858399486374 0.0 50-51 0.0 0.0 0.0 0.22513035599637582 0.0 52-53 0.0 0.0 0.0 0.27337257513845636 0.0 54-55 0.0 0.0 0.0 0.31939469215583915 0.0 56-57 0.0 0.0 0.0 0.40933882958616097 0.0 58-59 0.0 0.0 0.0 0.5240041041887926 0.0 60-61 0.0 0.0 0.0 0.6480898121313581 0.0 62-63 0.0 0.0 0.0 0.7741556111581134 0.0 64-65 0.0 0.0 0.0 0.9316628564913987 0.0 66-67 0.0 0.0 0.0 1.1305120035521634 0.0 68-69 0.0 0.0 0.0 1.3497020862959697 0.0 70-71 0.0 0.0 0.0 1.5427309656244188 0.0 72-73 0.0 0.0 0.0 1.7043183986463377 0.0 74-75 0.0 0.0 0.0 1.8718461049208264 0.0 76-77 0.0 0.0 0.0 2.0840758674899043 0.0 78-79 0.0 0.0 0.0 2.3039859833552345 0.0 80-81 0.0 0.0 0.0 2.576818533652548 0.0 82-83 0.0 0.0 0.0 2.8600315614518266 0.0 84-85 0.0 0.0 0.0 3.2437492124637735 0.0 86-87 0.0 0.0 0.0 3.6894497146868757 0.0 88-89 0.0 0.0 0.0 4.233074721437186 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4705 0.0 37.98006 1 GTATCAA 7290 0.0 33.05277 1 AGGTAAG 465 0.0 28.602095 2 GTAAGGT 535 0.0 26.632265 4 ATCAACG 9085 0.0 26.401777 3 TCAACGC 9235 0.0 26.022816 4 TATCAAC 9335 0.0 25.899792 2 CAACGCA 9350 0.0 25.702747 5 TAAGGTG 595 0.0 25.543106 5 AACGCAG 9535 0.0 25.154247 6 GTACATG 5080 0.0 24.885443 1 GGTGTGC 880 0.0 24.826519 8 GAGTACT 6105 0.0 24.779385 12-13 TACATGG 5190 0.0 24.43637 2 ACATGGG 5095 0.0 23.771835 3 AAGGTAA 560 0.0 23.76279 1 GGTAAGG 600 0.0 23.748531 3 GTACTTT 6390 0.0 23.52554 14-15 ACGCAGA 10720 0.0 22.285063 7 CGCAGAG 10775 0.0 22.127232 8 >>END_MODULE