FastQCFastQC Report
Thu 26 May 2016
SRR936736_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936736_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880524
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57790.6563137404545475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45300.5144663859247448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26610.30220641345380705No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12040.13673676129213969No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11970.13594178012183655No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10500.11924717554547065No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9540.10834457663845619No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA8970.10187115853741635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69250.033.290121
GGTATCA44700.032.8582421
ACCGATG1056.866341E-527.1646888
ATCAACG84800.027.1311113
TCAACGC85400.026.8848324
TATCAAC85450.026.8660492
CAACGCA86500.026.4894945
AACGCAG88100.026.1702946
GTACATG54600.025.942811
TACATGG55050.024.8658832
GAGTACT50800.023.71961412-13
ACATGGG54200.023.5923423
ACGCAGA98250.023.418317
CGCAGAG99550.023.160258
TAAGGTG5600.022.9202065
AGGTAAG4850.022.5400472
TAGCCCT4450.022.4324844
GTACTTT53700.022.34570514-15
GTGTAAG3900.021.9382131
CATGGGA32250.021.8147414
Produced by FastQC (version 0.11.5)