FastQCFastQC Report
Thu 26 May 2016
SRR936736_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936736_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880524
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67700.7688603604217489No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59570.6765289759279701No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37650.42758630088447336No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16340.1855713188964753No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16040.18216425673803327No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC13870.15751984045863598No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12390.14071166714365535No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA12030.13662319255352495No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11360.12901408706633777No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA10960.12447133752174842No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA8850.10050833367403955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49500.034.845671
GTATCAA76300.032.695061
AGGTAAG5400.029.0248622
GTAAGGT6100.028.0236034
TAAGGTG6350.027.6680985
GTATAGG1955.329639E-1026.8043611
ATCAACG95250.026.0274053
TATCAAC96800.025.7592932
TCAACGC97150.025.5140294
CAACGCA97350.025.510395
GGTAAGG6350.025.4290713
AACGCAG99650.024.9692386
ATGGGAG12400.024.8910375
AAGGTAA6200.024.524811
GAGTACT61300.023.58997512-13
GTACATG53850.023.4716321
AAGGTGT8850.023.0715376
AGTACTT63050.022.55861312-13
CATGGGA32000.022.5551094
ACGCAGA110600.022.497157
Produced by FastQC (version 0.11.5)