##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936736_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 880524 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77989356337817 31.0 31.0 34.0 30.0 34.0 2 31.93107399684733 31.0 31.0 34.0 30.0 34.0 3 31.977412313576917 31.0 31.0 34.0 30.0 34.0 4 35.74260781080357 37.0 35.0 37.0 35.0 37.0 5 35.563405426768604 37.0 35.0 37.0 33.0 37.0 6 35.6502014709423 37.0 35.0 37.0 33.0 37.0 7 35.582764353952875 37.0 35.0 37.0 33.0 37.0 8 35.54816336635912 37.0 35.0 37.0 33.0 37.0 9 37.139208017044396 39.0 37.0 39.0 33.0 39.0 10-11 37.03253176517619 39.0 37.0 39.0 33.0 39.0 12-13 36.91930430062099 39.0 37.0 39.0 33.0 39.0 14-15 37.97660654337644 40.0 37.0 41.0 33.0 41.0 16-17 37.88831650244627 40.0 37.0 41.0 33.0 41.0 18-19 38.055600983051 40.0 37.0 41.0 33.0 41.0 20-21 38.071849830328304 40.0 37.0 41.0 33.0 41.0 22-23 37.984066305972355 40.0 37.0 41.0 33.0 41.0 24-25 37.833517314689885 40.0 37.0 41.0 32.5 41.0 26-27 37.587951606089106 39.0 36.5 41.0 32.0 41.0 28-29 37.47380764181328 39.0 36.0 41.0 32.0 41.0 30-31 37.36517460057875 39.0 36.0 41.0 32.0 41.0 32-33 37.12021307766739 39.0 35.5 41.0 31.0 41.0 34-35 36.89998285112047 39.0 35.0 41.0 31.0 41.0 36-37 36.61527226969395 39.0 35.0 41.0 30.5 41.0 38-39 36.404410328395365 39.0 35.0 40.0 30.0 41.0 40-41 36.16375078930274 39.0 35.0 40.0 30.0 41.0 42-43 35.8196778282023 38.0 35.0 40.0 29.0 41.0 44-45 35.40652952105792 38.0 35.0 40.0 27.5 41.0 46-47 34.86894281132598 38.0 34.0 40.0 25.0 41.0 48-49 34.342256429126294 38.0 33.5 40.0 23.5 41.0 50-51 33.97663323203002 38.0 33.0 40.0 22.5 41.0 52-53 33.578359022582006 38.0 33.0 40.0 20.0 41.0 54-55 33.355954522534304 37.0 33.0 40.0 19.5 41.0 56-57 33.02787090414344 37.0 32.0 40.0 18.0 41.0 58-59 32.64530949752647 36.5 32.0 39.5 16.0 41.0 60-61 32.210038567943634 36.0 31.0 39.0 13.0 40.5 62-63 31.85756152018571 35.5 31.0 39.0 9.0 40.0 64-65 31.427109880025984 35.0 30.5 38.5 8.0 40.0 66-67 30.999632037286887 35.0 30.0 38.0 7.0 40.0 68-69 30.629242360230954 35.0 29.5 37.5 4.0 40.0 70-71 30.253983991350605 34.0 29.0 37.0 2.0 39.0 72-73 29.80437046576811 34.0 29.0 36.5 2.0 39.0 74-75 29.30193725554329 34.0 28.5 36.0 2.0 39.0 76-77 27.833548546093006 32.0 26.5 34.5 2.0 36.5 78-79 28.595650430879793 33.5 28.0 35.0 2.0 37.0 80-81 28.37829917185676 34.0 27.0 35.0 2.0 37.0 82-83 28.070459181123965 33.0 27.0 35.0 2.0 36.0 84-85 27.70107799446693 33.0 26.5 35.0 2.0 36.0 86-87 27.307788884800416 33.0 26.0 35.0 2.0 35.5 88-89 26.828205136941186 33.0 25.0 35.0 2.0 35.0 90-91 26.31505217347852 32.0 24.5 34.5 2.0 35.0 92-93 25.59296339452417 32.0 21.5 34.0 2.0 35.0 94-95 25.045106095915614 32.0 19.5 34.0 2.0 35.0 96-97 24.360152590957203 31.0 12.5 34.0 2.0 35.0 98-99 23.52472561792751 31.0 2.0 34.0 2.0 35.0 100-101 20.549348456146568 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4364863099217082 1101 2 0.41313264849505416 1101 3 0.42243097917469186 1101 4 0.3963926936116877 1101 5 0.24440874064598006 1101 6 0.3110731399497624 1101 7 0.3104841217420997 1101 8 0.35168875653408094 1101 9 0.3260339659646334 1101 10-11 0.42237702357897433 1101 12-13 0.5298756844802028 1101 14-15 0.6512083467646406 1101 16-17 0.5957646051242804 1101 18-19 0.660341747531433 1101 20-21 0.6349208451674642 1101 22-23 0.5634333221983567 1101 24-25 0.6238326326931016 1101 26-27 0.7691465362213492 1101 28-29 0.6929807838193724 1101 30-31 0.6853143596849378 1101 32-33 0.8338765407880189 1101 34-35 0.9334266988694893 1101 36-37 0.9617680274206677 1101 38-39 0.8424687432714961 1101 40-41 0.9711254305771817 1101 42-43 1.1097958033530233 1101 44-45 1.1979878406175715 1101 46-47 1.0958604215493395 1101 48-49 1.2484640391461213 1101 50-51 1.1341823229832713 1101 52-53 1.4203702557315907 1101 54-55 1.3124204368589432 1101 56-57 1.1299481528049498 1101 58-59 0.9212039586591629 1101 60-61 1.1469722358835064 1101 62-63 1.1437557369614986 1101 64-65 1.1710762651734576 1101 66-67 0.9677185672387516 1101 68-69 0.899142191300303 1101 70-71 1.031202761909352 1101 72-73 1.3195337023850975 1101 74-75 1.294648455492215 1101 76-77 1.0125186678182239 1101 78-79 1.0770894818457037 1101 80-81 1.1482550392384674 1101 82-83 1.1033156446499568 1101 84-85 1.1753728477515857 1101 86-87 1.1508543018137551 1101 88-89 1.2558049184333093 1101 90-91 1.054405084327822 1101 92-93 0.9439068158121202 1101 94-95 0.9505979804351412 1101 96-97 1.3351064567530884 1101 98-99 1.065150578816315 1101 100-101 1.3383258744363609 1102 1 0.2764317454790799 1102 2 0.29237650234126633 1102 3 0.27874914929283534 1102 4 0.30264776083639333 1102 5 0.2596007764144943 1102 6 0.21001269384121457 1102 7 0.24942030787806146 1102 8 0.3600143130358333 1102 9 0.455779581362421 1102 10-11 0.3143048141386018 1102 12-13 0.32314142079968633 1102 14-15 0.5589868414346242 1102 16-17 0.5222332923686466 1102 18-19 0.3631128036572875 1102 20-21 0.32746206048496873 1102 22-23 0.2952222126873423 1102 24-25 0.39360076216005524 1102 26-27 0.4620535839017421 1102 28-29 0.4121405911882903 1102 30-31 0.5810072045085164 1102 32-33 0.6125673796993141 1102 34-35 0.6210996932123365 1102 36-37 0.5050125026574577 1102 38-39 0.5608080145889929 1102 40-41 0.6603445308535214 1102 42-43 0.6353597039622798 1102 44-45 0.7936715006534314 1102 46-47 0.7887600987210632 1102 48-49 1.1113677587583624 1102 50-51 1.0993273292368642 1102 52-53 1.2471467184090343 1102 54-55 1.076656999571199 1102 56-57 0.9907868543254281 1102 58-59 1.1694788344093965 1102 60-61 1.216275994956316 1102 62-63 1.3189834856793148 1102 64-65 0.9574820422915451 1102 66-67 0.8843120485341025 1102 68-69 1.0304669381800302 1102 70-71 1.0876617087430382 1102 72-73 1.3201555181836113 1102 74-75 1.1094545033914507 1102 76-77 0.946520440142077 1102 78-79 1.0185405705832267 1102 80-81 1.0275758582040346 1102 82-83 0.8808429353757035 1102 84-85 0.8545134861283508 1102 86-87 1.0261689658376234 1102 88-89 1.1308061940982732 1102 90-91 1.0338864386417796 1102 92-93 1.0110175650753526 1102 94-95 0.62158740318824 1102 96-97 0.8695533794523271 1102 98-99 0.8802634950090962 1102 100-101 1.8492370620747671 1103 1 0.21569918504390273 1103 2 0.305661129101491 1103 3 0.29799751680727127 1103 4 0.25209813563446914 1103 5 0.23198448471145383 1103 6 0.29702830287411786 1103 7 0.22556938028852613 1103 8 0.18290654725948485 1103 9 0.32140202429837217 1103 10-11 0.318668507957689 1103 12-13 0.3560233243293851 1103 14-15 0.33112861034246777 1103 16-17 0.31132478730201996 1103 18-19 0.2964511140176569 1103 20-21 0.33985299530102253 1103 22-23 0.14628762758514569 1103 24-25 0.34036618090703996 1103 26-27 0.16690599901186687 1103 28-29 0.28930901254153696 1103 30-31 0.363501086179852 1103 32-33 0.2571933700863056 1103 34-35 0.29563316677160145 1103 36-37 0.22810706699893757 1103 38-39 0.35560948398121184 1103 40-41 0.4083488568316156 1103 42-43 0.4088028981830476 1103 44-45 0.3376778356534942 1103 46-47 0.5007396993255071 1103 48-49 0.510186247720533 1103 50-51 0.6441517695230985 1103 52-53 0.6649641544350899 1103 54-55 0.6197992874970808 1103 56-57 0.5308687362500706 1103 58-59 0.45910688596850235 1103 60-61 0.5883430153333684 1103 62-63 0.6537763502988021 1103 64-65 0.4984860193603815 1103 66-67 0.3706820823199415 1103 68-69 0.534301680601807 1103 70-71 0.4620515831729577 1103 72-73 0.6096860986518955 1103 74-75 0.5087578644678317 1103 76-77 0.3943009240705244 1103 78-79 0.4454181617931958 1103 80-81 0.37638624526980635 1103 82-83 0.35265997655071857 1103 84-85 0.7830204587259395 1103 86-87 1.0027645738026862 1103 88-89 0.8857700801278696 1103 90-91 0.9127183873061782 1103 92-93 0.672006902855518 1103 94-95 0.7467084660080481 1103 96-97 0.8568442667124181 1103 98-99 0.8166817676723639 1103 100-101 1.0001018231091798 1104 1 0.1989113330090717 1104 2 0.23153941930181077 1104 3 0.2447571109624036 1104 4 0.2195395968299252 1104 5 0.11303868086043423 1104 6 0.07633082129243007 1104 7 0.09822465524690926 1104 8 0.06732304997963467 1104 9 0.2528660281553883 1104 10-11 0.1467583278317477 1104 12-13 0.11282462263039861 1104 14-15 0.17054766800119125 1104 16-17 0.21728473193329734 1104 18-19 0.2573176322598414 1104 20-21 0.19991654870441522 1104 22-23 0.18174318182921212 1104 24-25 0.1422047006485201 1104 26-27 0.1929095755558663 1104 28-29 0.38346007175876196 1104 30-31 0.25303949147636473 1104 32-33 0.31687366420210594 1104 34-35 0.17507022185429122 1104 36-37 0.1880579884973841 1104 38-39 0.2931825846406113 1104 40-41 0.27556438819408413 1104 42-43 0.3905090939368563 1104 44-45 0.35503125229644894 1104 46-47 0.4558911844274931 1104 48-49 0.44802908773180405 1104 50-51 0.47604291618915084 1104 52-53 0.5209645969704368 1104 54-55 0.6163964645212019 1104 56-57 0.5308267397940512 1104 58-59 0.5419398077858943 1104 60-61 0.60404561974266 1104 62-63 0.5507042817901855 1104 64-65 0.5338438902177671 1104 66-67 0.605761407520145 1104 68-69 0.5248937344283142 1104 70-71 0.7233687030375044 1104 72-73 0.7956554712100399 1104 74-75 0.6439019049117114 1104 76-77 0.5730568965400877 1104 78-79 0.4485199793332093 1104 80-81 0.7093704098307931 1104 82-83 0.7988486409117108 1104 84-85 0.7974439287876436 1104 86-87 0.8998014740720208 1104 88-89 0.8080318649439491 1104 90-91 0.749170867113655 1104 92-93 0.6968162629960339 1104 94-95 0.48157373878705556 1104 96-97 0.6643258520989868 1104 98-99 0.6608567852202256 1104 100-101 1.6383502711828726 1105 1 0.07083810000737856 1105 2 0.22050460699513863 1105 3 0.13867984272296852 1105 4 0.22194062466815723 1105 5 0.3327169100776146 1105 6 0.21817372466156826 1105 7 0.26536169042846325 1105 8 0.3063860966738403 1105 9 0.13895673459585822 1105 10-11 0.24860558468695615 1105 12-13 0.35653343390677605 1105 14-15 0.3779610598342984 1105 16-17 0.31540650016635396 1105 18-19 0.3062797130184265 1105 20-21 0.38820552952122256 1105 22-23 0.33590852836339735 1105 24-25 0.41795531105731243 1105 26-27 0.2360112124571856 1105 28-29 0.2138792765817783 1105 30-31 0.3054616965217818 1105 32-33 0.20790194155788555 1105 34-35 0.34238951794942807 1105 36-37 0.2869772662719896 1105 38-39 0.44104363949688974 1105 40-41 0.32425520238570016 1105 42-43 0.5258094915583982 1105 44-45 0.47257443184280845 1105 46-47 0.4666495362033345 1105 48-49 0.5905500394737402 1105 50-51 0.717382807767514 1105 52-53 0.6469106656828032 1105 54-55 0.7264506564513908 1105 56-57 0.6631712998015686 1105 58-59 0.609683497946115 1105 60-61 0.697073289876851 1105 62-63 0.7000727666117719 1105 64-65 0.6281320409447098 1105 66-67 0.551768264496797 1105 68-69 0.5048004130631334 1105 70-71 0.7399820686174827 1105 72-73 0.7941768441352295 1105 74-75 0.6165249935920336 1105 76-77 0.5514426881214298 1105 78-79 0.677966874121914 1105 80-81 0.752550664457786 1105 82-83 0.8973165160091554 1105 84-85 1.0965352684195224 1105 86-87 1.0673981750550503 1105 88-89 0.9447377019677496 1105 90-91 0.9164911075658502 1105 92-93 0.8959209652803892 1105 94-95 1.0688970200690022 1105 96-97 1.0072086814683026 1105 98-99 0.7191465168008939 1105 100-101 0.9575963153891713 1106 1 0.08814060331251028 1106 2 0.15095333871573047 1106 3 0.02783422140523939 1106 4 0.200924793048884 1106 5 0.10950683333881983 1106 6 -0.12367292602588975 1106 7 -0.12580877083254904 1106 8 0.037119664277526 1106 9 0.020607238351843193 1106 10-11 -0.029585788410066982 1106 12-13 -0.019394820330731477 1106 14-15 0.014345387757991546 1106 16-17 -0.1002630229546142 1106 18-19 0.0542317156189398 1106 20-21 0.028116055932052575 1106 22-23 0.009197909539700788 1106 24-25 0.04779569958989782 1106 26-27 0.16109015882596367 1106 28-29 -0.25179469904588103 1106 30-31 -0.05737899029761451 1106 32-33 0.03941753124725267 1106 34-35 0.0782067272925886 1106 36-37 -0.09503317438917946 1106 38-39 0.31476245404656567 1106 40-41 0.34568608235670695 1106 42-43 0.34178458741183704 1106 44-45 0.5901520564709628 1106 46-47 0.6222721180718551 1106 48-49 0.4383141649733986 1106 50-51 0.5746208088846885 1106 52-53 0.18951897524348027 1106 54-55 0.4195919902231253 1106 56-57 0.32639178855603035 1106 58-59 0.3492339629061334 1106 60-61 0.37797458932380223 1106 62-63 0.32701279850790854 1106 64-65 0.11672988742601476 1106 66-67 0.2968980628511737 1106 68-69 0.36609175209691713 1106 70-71 0.3484448204409851 1106 72-73 0.4810915185386726 1106 74-75 0.3274361881902408 1106 76-77 0.12156593226140089 1106 78-79 0.14818178385434777 1106 80-81 0.29791485771027837 1106 82-83 0.37959398252661813 1106 84-85 0.7668470746613778 1106 86-87 0.933152607167397 1106 88-89 0.9024033928193127 1106 90-91 0.9173364611104482 1106 92-93 0.9875660077099759 1106 94-95 0.8541133900990836 1106 96-97 0.9268863947106816 1106 98-99 0.7687655005458183 1106 100-101 1.1605390798166972 1107 1 0.14553449394155038 1107 2 0.20030341582522482 1107 3 0.1149056889761475 1107 4 0.20669124227634939 1107 5 0.12012016513953938 1107 6 -0.057655703308768125 1107 7 0.05996285095734777 1107 8 0.14441680883148678 1107 9 0.05310098817223974 1107 10-11 0.12702671003336974 1107 12-13 0.10315779133435399 1107 14-15 0.15772594467054546 1107 16-17 0.22199240584123459 1107 18-19 0.010899531743923774 1107 20-21 0.23867526740365008 1107 22-23 0.27508198666834716 1107 24-25 0.28544860149321494 1107 26-27 0.3366016677773658 1107 28-29 0.26401873429032463 1107 30-31 0.3292604725222006 1107 32-33 0.2532910142017357 1107 34-35 0.45797218990167465 1107 36-37 0.45535859932079603 1107 38-39 0.33294784251557985 1107 40-41 0.28872323859682325 1107 42-43 0.3035639009936517 1107 44-45 0.5113985961224827 1107 46-47 0.2032047598144331 1107 48-49 0.36781644952180415 1107 50-51 0.4621235627952416 1107 52-53 0.39173144303470053 1107 54-55 0.4099497112304391 1107 56-57 0.3144613690995328 1107 58-59 0.43571633308919644 1107 60-61 0.3595425462574333 1107 62-63 0.5283140811118159 1107 64-65 0.4739348358440729 1107 66-67 0.7167161610723873 1107 68-69 0.7786826311451698 1107 70-71 0.6957823876124571 1107 72-73 0.7236942753228703 1107 74-75 0.5398512959040644 1107 76-77 0.23695119237423867 1107 78-79 0.7638975132335446 1107 80-81 0.8491806986827193 1107 82-83 0.7774861633364125 1107 84-85 1.044881884090767 1107 86-87 0.6524800303692153 1107 88-89 0.7140227401185655 1107 90-91 0.8925524634859947 1107 92-93 0.9028593111573926 1107 94-95 1.199950674695387 1107 96-97 1.6185022936349789 1107 98-99 1.405901864617583 1107 100-101 1.8333062570344332 1108 1 -0.08907294769098684 1108 2 0.049226199261816816 1108 3 -0.06252439258078724 1108 4 -0.018631660170356668 1108 5 -0.023623928108385428 1108 6 -0.0801757425120968 1108 7 0.03754344530786824 1108 8 -0.04277472744909261 1108 9 -0.10903279319917658 1108 10-11 -0.08284456652513228 1108 12-13 -0.06428331615978777 1108 14-15 -0.12303948289079614 1108 16-17 -0.12522398255178047 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-0.6685550657090005 2316 94-95 -1.0399457382492336 2316 96-97 -1.0327829664247474 2316 98-99 -1.731415434742921 2316 100-101 -2.47954150973894 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 14.0 5 42.0 6 95.0 7 228.0 8 612.0 9 1133.0 10 1866.0 11 2779.0 12 3747.0 13 5122.0 14 6523.0 15 8792.0 16 11370.0 17 14370.0 18 12360.0 19 8031.0 20 7130.0 21 7244.0 22 7425.0 23 8161.0 24 9421.0 25 11256.0 26 13408.0 27 16111.0 28 19613.0 29 24134.0 30 28883.0 31 35006.0 32 43000.0 33 53327.0 34 69439.0 35 90676.0 36 122166.0 37 148425.0 38 83890.0 39 4716.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.82658833471913 19.531416835916378 15.32879880412015 21.313196025244334 2 16.298946531504672 21.00428770196212 38.960502030269474 23.736263736263734 3 19.413859919721773 25.667565461736093 30.409977761068923 24.50859685747321 4 12.997260722024611 17.398957893254472 36.42422012347193 33.179561261248985 5 13.123186389474103 38.34365896455971 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21.3283741003109 25.76732300790733 31.73401854033872 21.17028435144305 28-29 21.06878560460827 26.0510457646984 31.705477771821595 21.174690858871735 30-31 21.361386110502245 25.523136102268353 31.753750963933282 21.361726823296124 32-33 21.036329634170787 25.775293622565027 31.52183974889386 21.66653699437033 34-35 21.76356048805889 25.68322736237072 31.328147314875437 21.22506483469495 36-37 22.082752472267728 25.354919124264992 31.23827749022592 21.32405091324136 38-39 21.669716966614406 25.733761340670707 31.10721061379455 21.489311078920338 40-41 21.56667561970448 25.827280568709078 31.122273700612308 21.483770110974135 42-43 21.670962674984963 25.946470543807077 30.61426956217059 21.768297219037365 44-45 21.646021176628253 26.132965511601945 30.438592207373322 21.782421104396484 46-47 22.366341688060288 26.653129203839327 29.528647576799834 21.451881531300558 48-49 22.52554184137483 26.812289612000235 28.714015418232037 21.948153128392896 50-51 22.228134122789733 27.178723063548137 28.756651597945083 21.83649121571705 52-53 22.794717224555434 27.074678429386207 28.434383240850313 21.696221105208046 54-55 22.055266074884383 27.274890290106573 28.565060465369836 22.104783169639205 56-57 22.43896067016896 27.259633678830042 28.270850489848076 22.03055516115292 58-59 22.490001584750946 26.80501144826356 28.28814521089968 22.416841756085812 60-61 22.094501990493114 26.981930786842582 28.994064019529496 21.929503203134807 62-63 22.08679374516186 27.17587799283146 29.01878689126048 21.7185413707462 64-65 21.772356800045436 27.2225030528498 28.712123363530512 22.29301678357425 66-67 22.006080684343885 27.184202486561503 28.46053290599698 22.34918392309763 68-69 22.31114134601079 26.905905988895114 28.616045418977347 22.16690724611675 70-71 22.255365422235094 27.068019272647774 28.32477307507758 22.35184223003955 72-73 22.277643766666213 27.13134451758271 28.195029323448313 22.395982392302766 74-75 22.07051480074867 27.6749127764351 27.910518616779633 22.344053806036598 76-77 22.20572243059913 27.292989923138734 28.11032587246197 22.39096177380016 78-79 22.27180207133592 27.529594569728687 27.83087876165098 22.36772459728441 80-81 22.35849726380239 27.93693256202947 27.420988211773462 22.283581962394678 82-83 22.381442203979674 27.738772700396513 27.40587425742518 22.473910838198634 84-85 21.98366703287839 27.80694469218333 27.43622633020633 22.77316194473195 86-87 22.160070924040056 27.783050131278763 27.085225275265127 22.971653669416053 88-89 21.862091493985144 27.765370904287806 26.98791347697027 23.38462412475678 90-91 21.970913313766463 27.771149434299293 27.1981764858947 23.05976076603955 92-93 22.006806334173852 27.39353528904198 27.508053480449824 23.09160489633434 94-95 21.559400330271664 27.38340654715006 27.577740803206602 23.479452319371678 96-97 21.790740168360575 27.267231504881728 27.584328012217963 23.357700314539738 98-99 21.468222898240775 27.534946459558018 27.10119103761524 23.895639604585966 100-101 21.311793056971386 27.42646315117174 27.244233367767773 24.017510424089107 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 656.0 1 480.0 2 425.5 3 528.5 4 448.0 5 395.5 6 594.5 7 1145.0 8 1783.5 9 2135.0 10 1941.0 11 1599.5 12 1526.0 13 1618.0 14 1751.5 15 1880.0 16 2042.0 17 2162.5 18 2309.5 19 2462.5 20 2642.5 21 2926.0 22 3276.0 23 3726.5 24 4275.0 25 5020.5 26 5949.0 27 7073.5 28 8367.0 29 9777.0 30 11315.5 31 13301.0 32 15676.5 33 17040.5 34 17340.5 35 18173.5 36 20256.5 37 22250.5 38 23269.0 39 23359.5 40 23420.5 41 24658.5 42 25446.5 43 26200.0 44 27399.0 45 28665.5 46 28996.5 47 29531.0 48 32817.0 49 32909.5 50 29853.5 51 28341.5 52 27890.0 53 28174.5 54 28484.0 55 27043.0 56 24259.0 57 22560.5 58 22117.5 59 19931.5 60 16166.5 61 13642.5 62 12148.5 63 9533.0 64 6512.0 65 4785.5 66 3598.5 67 2926.0 68 2269.0 69 1615.0 70 1235.5 71 964.0 72 795.5 73 744.5 74 621.0 75 480.0 76 368.0 77 238.0 78 154.5 79 117.5 80 91.0 81 77.0 82 65.5 83 50.0 84 27.0 85 13.0 86 5.5 87 4.0 88 2.5 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019079548087275307 2 0.011243305122858661 3 0.00976691152086712 4 0.0 5 0.0032934934198272842 6 0.0 7 1.1356873861473394E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0014763936019915414 14-15 3.4070621584420186E-4 16-17 0.0 18-19 0.0018738841871431102 20-21 0.0 22-23 5.678436930736697E-5 24-25 0.0 26-27 0.0013060404940694406 28-29 0.002271374772294679 30-31 0.001930668556450477 32-33 0.011300089492166028 34-35 0.001760315448528376 36-37 7.381968009957707E-4 38-39 0.0010789030168399725 40-41 3.974905851515688E-4 42-43 0.0063030649931177345 44-45 0.002839218465368349 46-47 5.110593237663028E-4 48-49 9.085499089178716E-4 50-51 2.839218465368349E-4 52-53 2.271374772294679E-4 54-55 0.002271374772294679 56-57 0.01692174205359536 58-59 0.029471087670523463 60-61 0.02436049443286043 62-63 0.016978526422902727 64-65 0.022600178984332055 66-67 0.003520630897056752 68-69 0.0012492561247620735 70-71 1.7035310792210093E-4 72-73 0.0 74-75 0.0031799246812125505 76-77 0.004713102652511459 78-79 0.014309661065456478 80-81 0.022316257137795222 82-83 0.025609750557622508 84-85 0.03872693986762428 86-87 0.01652425146844379 88-89 0.046733535939963024 90-91 0.05280946345585128 92-93 0.03571736829433383 94-95 0.03855658675970218 96-97 0.057692919216284846 98-99 0.060759275158882665 100-101 0.06711912452130776 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 880524.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.45951384323098 #Duplication Level Percentage of deduplicated Percentage of total 1 79.87433689326743 45.096662295443274 2 11.571201776105026 13.066088537216414 3 3.596208698607297 6.091205844064991 4 1.552174712967221 3.505401187755436 5 0.829430038544381 2.341460837159404 6 0.5180559103203433 1.754951090417943 7 0.35743330727959405 1.4126357532268852 8 0.25869804828726417 1.1684772830793304 9 0.1892836442417086 0.9618176279125762 >10 1.110377028389365 11.69143608718968 >50 0.09279172093516021 3.5548661184269483 >100 0.04378229797941525 4.853481753001978 >500 0.004217560793296153 1.5770442267473268 >1k 0.0016066898260175821 1.481342089317449 >5k 4.0167245650439554E-4 1.4431292690403532 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6770 0.7688603604217489 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5957 0.6765289759279701 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3765 0.42758630088447336 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1634 0.1855713188964753 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1604 0.18216425673803327 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1387 0.15751984045863598 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1239 0.14071166714365535 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1203 0.13662319255352495 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1136 0.12901408706633777 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1096 0.12447133752174842 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 885 0.10050833367403955 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.1356873861473396E-4 0.0 24-25 0.0 0.0 0.0 5.678436930736698E-4 0.0 26-27 0.0 0.0 0.0 0.0011924717554547067 0.0 28-29 0.0 0.0 0.0 0.0026120809881388807 0.0 30-31 0.0 0.0 0.0 0.004599533913896725 0.0 32-33 0.0 0.0 0.0 0.00806338044164611 0.0 34-35 0.0 0.0 0.0 0.010675461429784991 0.0 36-37 0.0 0.0 0.0 0.01561570155952592 0.0 38-39 0.0 0.0 0.0 0.024246925694245698 0.0 40-41 0.0 0.0 0.0 0.041225452117148426 0.0 42-43 0.0 0.0 0.0 0.06427990605593942 0.0 44-45 0.0 0.0 0.0 0.08733435999473041 0.0 46-47 0.0 0.0 0.0 0.13702068313867652 0.0 48-49 0.0 0.0 0.0 0.18477633772617214 0.0 50-51 0.0 0.0 0.0 0.2245821806106364 0.0 52-53 0.0 0.0 0.0 0.26643226079016585 0.0 54-55 0.0 0.0 0.0 0.30873661592415425 0.0 56-57 0.0 0.0 0.0 0.39379960114658996 0.0 58-59 0.0 0.0 0.0 0.5083904584088565 0.0 60-61 0.0 0.0 0.0 0.6261612403523357 0.0 62-63 0.0 0.0 0.0 0.7390485665353812 0.0 64-65 0.0 0.0 0.0 0.8778863494918935 0.0 66-67 0.0 0.0 0.0 1.0649340619903602 0.0 68-69 0.0 0.0 0.0 1.2798628998187445 0.0 70-71 0.0 0.0 0.0 1.4646960219142238 0.0 72-73 0.0 0.0 0.0 1.6313581458313458 0.0 74-75 0.0 0.0 0.0 1.8027333724009793 0.0 76-77 0.0 0.0 0.0 2.006873180060964 0.0 78-79 0.0 0.0 0.0 2.224186961400257 0.0 80-81 0.0 0.0 0.0 2.4839186666121535 0.0 82-83 0.0 0.0 0.0 2.7568811298726668 0.0 84-85 0.0 0.0 0.0 3.1264338053250107 0.0 86-87 0.0 0.0 0.0 3.552543712607493 0.0 88-89 0.0 0.0 0.0 4.057299971380678 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4950 0.0 34.84567 1 GTATCAA 7630 0.0 32.69506 1 AGGTAAG 540 0.0 29.024862 2 GTAAGGT 610 0.0 28.023603 4 TAAGGTG 635 0.0 27.668098 5 GTATAGG 195 5.329639E-10 26.804361 1 ATCAACG 9525 0.0 26.027405 3 TATCAAC 9680 0.0 25.759293 2 TCAACGC 9715 0.0 25.514029 4 CAACGCA 9735 0.0 25.51039 5 GGTAAGG 635 0.0 25.429071 3 AACGCAG 9965 0.0 24.969238 6 ATGGGAG 1240 0.0 24.891037 5 AAGGTAA 620 0.0 24.52481 1 GAGTACT 6130 0.0 23.589975 12-13 GTACATG 5385 0.0 23.471632 1 AAGGTGT 885 0.0 23.071537 6 AGTACTT 6305 0.0 22.558613 12-13 CATGGGA 3200 0.0 22.555109 4 ACGCAGA 11060 0.0 22.49715 7 >>END_MODULE