##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936735_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 271543 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.59772853654854 31.0 31.0 34.0 30.0 34.0 2 31.54948939946896 31.0 31.0 34.0 30.0 34.0 3 31.55802211804392 33.0 31.0 34.0 28.0 34.0 4 35.20874778580188 37.0 35.0 37.0 33.0 37.0 5 35.26093841491035 37.0 35.0 37.0 33.0 37.0 6 35.288488379372694 37.0 35.0 37.0 33.0 37.0 7 35.27182435194426 37.0 35.0 37.0 33.0 37.0 8 35.26575164891012 37.0 35.0 37.0 32.0 37.0 9 36.75807883097705 39.0 37.0 39.0 32.0 39.0 10-11 36.61537399233271 39.0 37.0 39.0 32.0 39.0 12-13 36.51306975322509 39.0 37.0 39.0 32.0 39.0 14-15 37.48079493855485 39.5 37.0 41.0 32.0 41.0 16-17 37.43373241070475 39.0 37.0 41.0 32.0 41.0 18-19 37.56783824293022 40.0 37.0 41.0 32.0 41.0 20-21 37.44594594594595 40.0 37.0 41.0 32.0 41.0 22-23 37.49728403972851 40.0 37.0 41.0 32.0 41.0 24-25 37.515999307660294 40.0 37.0 41.0 32.0 41.0 26-27 37.30996195814291 39.5 36.5 41.0 31.5 41.0 28-29 37.125556541689534 39.0 36.0 41.0 31.5 41.0 30-31 36.86696582125114 39.0 35.5 41.0 31.0 41.0 32-33 36.540794643942206 39.0 35.0 41.0 30.0 41.0 34-35 36.19713636514291 38.5 35.0 40.0 30.0 41.0 36-37 35.87262238393183 38.0 35.0 40.0 30.0 41.0 38-39 35.5628261453987 38.0 35.0 40.0 28.5 41.0 40-41 35.31188062295843 38.0 35.0 40.0 27.0 41.0 42-43 35.06907193335862 38.0 35.0 40.0 26.5 41.0 44-45 34.67427626563749 38.0 34.0 40.0 24.5 41.0 46-47 34.165574144794746 38.0 33.5 40.0 22.0 41.0 48-49 33.82613066807099 38.0 33.0 40.0 18.5 41.0 50-51 32.763904795925505 37.0 32.0 39.5 14.0 40.5 52-53 32.79629377299359 37.5 32.5 39.5 12.5 40.0 54-55 33.241716781504216 38.0 33.0 40.0 9.5 41.0 56-57 33.12991312609789 38.0 33.0 40.0 8.0 41.0 58-59 32.91582180354493 38.0 33.0 40.0 6.5 41.0 60-61 32.54857610028614 37.0 32.0 40.0 2.0 41.0 62-63 32.15654795004843 36.5 31.5 40.0 2.0 41.0 64-65 31.702726639979673 36.0 31.0 39.0 2.0 41.0 66-67 31.211281454502604 35.5 30.5 39.0 2.0 40.5 68-69 30.86617589111117 35.0 30.0 39.0 2.0 40.0 70-71 30.386401048820996 35.0 29.5 37.5 2.0 40.0 72-73 29.905002154354925 35.0 29.0 37.0 2.0 39.5 74-75 29.475219394350066 35.0 29.0 37.0 2.0 39.0 76-77 28.949906644619823 34.0 28.5 36.0 2.0 39.0 78-79 28.434540754134705 34.0 27.5 36.0 2.0 38.0 80-81 27.96075023108679 34.0 26.0 35.0 2.0 37.0 82-83 27.539859248811425 34.0 26.0 35.0 2.0 37.0 84-85 27.16130594417827 34.0 26.0 35.0 2.0 36.0 86-87 26.753965301996367 33.0 25.0 35.0 2.0 36.0 88-89 26.263545736770972 33.0 23.5 35.0 2.0 35.5 90-91 25.748934791174875 33.0 21.0 35.0 2.0 35.0 92-93 25.30234622140876 32.5 19.5 35.0 2.0 35.0 94-95 24.815224476418102 32.0 14.0 35.0 2.0 35.0 96-97 24.145873765849238 32.0 2.0 35.0 2.0 35.0 98-99 23.398804240948948 32.0 2.0 34.5 2.0 35.0 100-101 20.7887940399863 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27866900680455586 1101 2 0.48612235376124957 1101 3 0.25049369494059803 1101 4 0.3147034655752492 1101 5 0.25380020677645376 1101 6 0.3442620897577413 1101 7 0.36433285380282143 1101 8 0.43359350267836305 1101 9 0.417796517102488 1101 10-11 0.566056704275006 1101 12-13 0.6780532864123288 1101 14-15 0.5530322233423846 1101 16-17 0.5148192346254632 1101 18-19 0.5190874067013524 1101 20-21 0.4170144253794206 1101 22-23 0.5883742805421193 1101 24-25 0.42785989314278083 1101 26-27 0.3607147289255934 1101 28-29 0.31138840413350266 1101 30-31 0.15211094722933183 1101 32-33 0.24586157996418478 1101 34-35 0.28770835858505706 1101 36-37 0.09458479000529252 1101 38-39 0.488108779826085 1101 40-41 0.5817348915697735 1101 42-43 0.42958025447134673 1101 44-45 0.5424148729037199 1101 46-47 0.8071001240123437 1101 48-49 0.9125718188117133 1101 50-51 0.8711156115774941 1101 52-53 0.8609581156782085 1101 54-55 0.7277235017629451 1101 56-57 0.7989041297719197 1101 58-59 1.021426274230663 1101 60-61 0.8790413730749194 1101 62-63 0.8118904994087082 1101 64-65 0.6311529346009728 1101 66-67 0.6213698498459728 1101 68-69 0.8424210132281438 1101 70-71 0.7465299925137785 1101 72-73 0.5852800411761159 1101 74-75 0.9198262408266018 1101 76-77 0.8708938120404817 1101 78-79 0.8365669210342652 1101 80-81 0.4755447223421818 1101 82-83 0.5041956352675285 1101 84-85 0.581338872785178 1101 86-87 0.8342218059843383 1101 88-89 0.8952533096521407 1101 90-91 0.9480378080347478 1101 92-93 0.8773774008831339 1101 94-95 1.0282831737903386 1101 96-97 1.0626370228346325 1101 98-99 1.1546557348238444 1101 100-101 1.4109800661465073 1102 1 0.39782202436395053 1102 2 0.4252460333348935 1102 3 0.2510101476482838 1102 4 0.517040088582128 1102 5 0.25202951177866595 1102 6 0.3821367349677516 1102 7 0.5166169635511935 1102 8 0.622662933018006 1102 9 0.6590623904283532 1102 10-11 0.44713153349841406 1102 12-13 0.37727166344884466 1102 14-15 0.36334001179641007 1102 16-17 0.2519317865962307 1102 18-19 0.24517217438444305 1102 20-21 0.48609539995605644 1102 22-23 0.4080172771829993 1102 24-25 0.27352733231853676 1102 26-27 0.18918733089245166 1102 28-29 0.0492756351657917 1102 30-31 0.4745249947429784 1102 32-33 0.28964071180284634 1102 34-35 -0.07546639338744399 1102 36-37 0.18385081956895277 1102 38-39 0.27351182993501766 1102 40-41 0.0961543379671852 1102 42-43 0.11470902045316933 1102 44-45 0.10378377637481151 1102 46-47 0.07418429278387606 1102 48-49 0.46778234923901607 1102 50-51 0.31733726092780046 1102 52-53 0.4152095196483856 1102 54-55 0.24146157778130828 1102 56-57 0.42210414018776987 1102 58-59 0.32053919075628556 1102 60-61 0.35182561736139206 1102 62-63 0.5954729452245751 1102 64-65 0.7403588126139731 1102 66-67 0.8495616577776453 1102 68-69 0.8331812836931256 1102 70-71 0.8881487028575918 1102 72-73 0.9602420667123148 1102 74-75 0.7213131038320952 1102 76-77 1.037308762261386 1102 78-79 0.7102660244029231 1102 80-81 0.6847167488331642 1102 82-83 0.8118972905201538 1102 84-85 0.4368644827635677 1102 86-87 0.4103595209198012 1102 88-89 0.5830598178242461 1102 90-91 0.5842728243963151 1102 92-93 0.3129271409208023 1102 94-95 0.2612684440383255 1102 96-97 0.47845523148152935 1102 98-99 0.5443453424065865 1102 100-101 0.48867798905183335 1103 1 0.2600509959323283 1103 2 0.2756884629010017 1103 3 0.38329733232727037 1103 4 0.36735844928505657 1103 5 0.5365144523000538 1103 6 0.42686107876753 1103 7 0.5739318155866613 1103 8 0.03444748991846325 1103 9 0.22193304615711185 1103 10-11 0.4511836225699355 1103 12-13 0.18620065139430864 1103 14-15 0.08956717692214511 1103 16-17 0.3452787178769654 1103 18-19 0.7166661123797837 1103 20-21 0.6265612040959851 1103 22-23 0.2624547241435664 1103 24-25 0.5114889305240098 1103 26-27 0.5817213629816251 1103 28-29 0.7100738446039614 1103 30-31 0.4615746868040631 1103 32-33 0.5200291465996969 1103 34-35 0.5459101754633338 1103 36-37 0.25471647284114596 1103 38-39 0.3878809893932882 1103 40-41 0.3793439995569585 1103 42-43 0.12229459074811189 1103 44-45 0.6092477691093308 1103 46-47 0.7602851061793459 1103 48-49 0.9164401722771203 1103 50-51 1.0938942010918709 1103 52-53 1.4393756528189385 1103 54-55 1.2419876297611978 1103 56-57 1.2211944672829276 1103 58-59 1.1428676159253897 1103 60-61 1.4312743545885027 1103 62-63 1.3713996155636856 1103 64-65 1.5258611178134522 1103 66-67 1.5147916070086715 1103 68-69 1.3799701333762506 1103 70-71 1.5025923036337652 1103 72-73 1.2636599157552446 1103 74-75 1.4042303101983187 1103 76-77 1.455770705378292 1103 78-79 1.2853276866279266 1103 80-81 1.7717585004870031 1103 82-83 1.6732007083691975 1103 84-85 1.5433155673735683 1103 86-87 1.5617830870559501 1103 88-89 1.2482649532826322 1103 90-91 1.5294070917200528 1103 92-93 1.447504636814127 1103 94-95 1.5585175892038556 1103 96-97 1.850723855458579 1103 98-99 2.064898590281288 1103 100-101 2.5153552760131923 1104 1 0.40284847939040347 1104 2 0.32708232340647925 1104 3 0.31873501537315363 1104 4 0.4120758806767455 1104 5 0.3956803054294582 1104 6 0.4528183409434732 1104 7 0.45617500899158614 1104 8 0.5744680995290494 1104 9 0.5897657828964569 1104 10-11 0.7415215117119232 1104 12-13 0.6808041875695992 1104 14-15 0.6036061182389858 1104 16-17 0.611299978317362 1104 18-19 0.4994516639580482 1104 20-21 0.18739480860840274 1104 22-23 0.19716293544630759 1104 24-25 0.44550834694661035 1104 26-27 0.5552081836780118 1104 28-29 0.584040652283754 1104 30-31 0.45164139685937954 1104 32-33 0.4242812346786664 1104 34-35 0.34365125367138205 1104 36-37 0.6271592540538578 1104 38-39 0.46906116077846605 1104 40-41 0.3170460279765237 1104 42-43 0.6720152762888318 1104 44-45 0.6743253263281161 1104 46-47 0.01784286732480922 1104 48-49 0.6590288478384565 1104 50-51 0.3207219481360184 1104 52-53 0.29309622988803596 1104 54-55 -0.005799548891580741 1104 56-57 0.03445241283016287 1104 58-59 0.1525731154372636 1104 60-61 0.31030678760726715 1104 62-63 0.6484063408050282 1104 64-65 0.5569165468187656 1104 66-67 0.5394309388233971 1104 68-69 0.41568984267227194 1104 70-71 0.2546830943919858 1104 72-73 0.7526012326008917 1104 74-75 0.6622328050459139 1104 76-77 0.3342119679881179 1104 78-79 0.274567300468906 1104 80-81 0.15510268098380564 1104 82-83 -0.05918892174252832 1104 84-85 -0.003308252997403116 1104 86-87 0.34970748232658977 1104 88-89 0.4722832849888867 1104 90-91 0.4754804185450858 1104 92-93 0.2387130108243234 1104 94-95 0.003316374321549631 1104 96-97 0.3143017923280915 1104 98-99 0.15629678965215277 1104 100-101 0.1340639212024719 1105 1 0.10171614985190658 1105 2 0.045309768626211167 1105 3 0.29794911746643393 1105 4 0.22273201973905543 1105 5 0.22403750949360557 1105 6 0.3479952291794888 1105 7 0.3612551901643144 1105 8 0.4560998782045331 1105 9 0.5828432709610922 1105 10-11 0.4444992323631638 1105 12-13 0.5287353908992571 1105 14-15 -0.005302560913911236 1105 16-17 0.3205116321305539 1105 18-19 0.2482581274174649 1105 20-21 0.4303774258198132 1105 22-23 0.14154719300537266 1105 24-25 0.15239107433062316 1105 26-27 0.25764116201073506 1105 28-29 0.4376212619054982 1105 30-31 0.15537808433642653 1105 32-33 0.15401559628963923 1105 34-35 0.044700953757065065 1105 36-37 0.47982787934459026 1105 38-39 0.3454352579751827 1105 40-41 0.35096388817972723 1105 42-43 0.17744739772352602 1105 44-45 0.08166404587763765 1105 46-47 0.40758763412612353 1105 48-49 0.3491775808535138 1105 50-51 0.5080306762545135 1105 52-53 0.44832330786575625 1105 54-55 0.18184275738826017 1105 56-57 -0.026347562687035975 1105 58-59 0.1624611066234607 1105 60-61 0.24661478124173897 1105 62-63 0.3415174927208078 1105 64-65 0.37576830345972567 1105 66-67 0.4970573312826936 1105 68-69 0.1683768300636288 1105 70-71 0.13774405656605637 1105 72-73 0.04687837790631377 1105 74-75 0.29599764774392057 1105 76-77 0.33735188719487397 1105 78-79 0.280528862471602 1105 80-81 0.25602690671522765 1105 82-83 -0.12603920941543478 1105 84-85 0.20823232724497487 1105 86-87 0.21926005914137292 1105 88-89 0.1546220045384068 1105 90-91 0.6188455795195011 1105 92-93 0.1626143451369657 1105 94-95 0.5690404531561697 1105 96-97 1.0831872128201923 1105 98-99 0.969358363896248 1105 100-101 1.1345382754686 1106 1 0.34761864179062485 1106 2 -0.18560691413825126 1106 3 0.09065904510065081 1106 4 0.0953266376269255 1106 5 0.13128448929953862 1106 6 0.2339634001232156 1106 7 0.019538620332077983 1106 8 0.36289364315690875 1106 9 0.2239936937139646 1106 10-11 0.495615924043328 1106 12-13 0.14440176532902882 1106 14-15 0.43956125173747296 1106 16-17 0.4707412114580194 1106 18-19 0.36423283654269056 1106 20-21 0.15807331122331902 1106 22-23 0.26872225281487516 1106 24-25 0.3498367256969601 1106 26-27 0.17046417046578455 1106 28-29 0.20241389753561378 1106 30-31 0.3782929245224693 1106 32-33 -0.17215421811516762 1106 34-35 -0.5193063571186016 1106 36-37 -0.41167917148976585 1106 38-39 -0.24310509300628524 1106 40-41 -0.13835584375483023 1106 42-43 -0.004250894919529458 1106 44-45 -0.16297167339662622 1106 46-47 0.09375120753950483 1106 48-49 -0.04066149072471603 1106 50-51 -0.44369451208969224 1106 52-53 -0.4385029993990557 1106 54-55 -0.28684826895764814 1106 56-57 -0.22269732567795586 1106 58-59 0.08256898661811363 1106 60-61 0.18538248262515822 1106 62-63 -0.12108856698467463 1106 64-65 -0.12815089056080708 1106 66-67 0.425943462908176 1106 68-69 0.32957643777274015 1106 70-71 0.15168970050263653 1106 72-73 0.020740511433810838 1106 74-75 0.5996330252496023 1106 76-77 0.3906529258741642 1106 78-79 0.058327278448533804 1106 80-81 0.676751456100785 1106 82-83 0.4032525866527372 1106 84-85 0.2866793605451221 1106 86-87 0.30597640608106147 1106 88-89 0.5935122968248372 1106 90-91 0.5592403965247179 1106 92-93 0.5128528401664632 1106 94-95 0.19965548800170296 1106 96-97 0.5882282993470831 1106 98-99 0.8375657134066259 1106 100-101 0.349209806146046 1107 1 -0.06222853064209488 1107 2 0.3982424194923908 1107 3 0.2832114884549668 1107 4 0.47866483079685196 1107 5 0.2675257201532162 1107 6 0.30192106808774355 1107 7 0.3545500266544366 1107 8 0.43339843724277216 1107 9 0.4430722686955235 1107 10-11 0.5628617688830388 1107 12-13 0.5545147994107751 1107 14-15 0.2722828174052978 1107 16-17 0.33479157080005706 1107 18-19 0.1717704431017566 1107 20-21 0.08120927801634537 1107 22-23 0.38456932233341234 1107 24-25 0.630166394141753 1107 26-27 0.4555579081375285 1107 28-29 0.6552148786508596 1107 30-31 0.62695932818621 1107 32-33 0.5724513632642214 1107 34-35 0.9149915108424906 1107 36-37 1.0999372673363226 1107 38-39 0.609770500894335 1107 40-41 0.9052154500480256 1107 42-43 1.3970929928353968 1107 44-45 1.5228699073652763 1107 46-47 1.3851913200590147 1107 48-49 1.813363311594287 1107 50-51 1.921912424326493 1107 52-53 1.6159943505606336 1107 54-55 1.7518731937959942 1107 56-57 1.6302168610426833 1107 58-59 1.6754747686801679 1107 60-61 1.7736528381936765 1107 62-63 1.8012712553168306 1107 64-65 1.915274608144184 1107 66-67 2.208224212809551 1107 68-69 1.8809434812196777 1107 70-71 1.8985894106276788 1107 72-73 1.756748470130919 1107 74-75 1.8671112846276579 1107 76-77 1.5778109506078728 1107 78-79 1.6197842002852134 1107 80-81 1.8196992614557566 1107 82-83 1.9835127880214962 1107 84-85 1.7545658461971705 1107 86-87 1.8664342026883212 1107 88-89 1.7634836523761983 1107 90-91 2.231231442990474 1107 92-93 2.485434785587639 1107 94-95 2.494513209138564 1107 96-97 3.0708829707935372 1107 98-99 3.2081125307853995 1107 100-101 2.496064062505287 1108 1 0.3017572095491339 1108 2 0.035812482136638124 1108 3 0.24522311061124924 1108 4 0.46366318226404957 1108 5 0.24855728955644452 1108 6 0.45668738856252133 1108 7 0.5585559613725408 1108 8 0.2437141312750839 1108 9 0.33669038607105506 1108 10-11 0.24152151171191605 1108 12-13 0.07614148915690322 1108 14-15 0.32994540395327476 1108 16-17 0.3356055338729149 1108 18-19 0.362546902053289 1108 20-21 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-2.1634012912230887 2316 80-81 -1.731700763798365 2316 82-83 -1.768712731266337 2316 84-85 -1.059311292511083 2316 86-87 -1.8405154153431198 2316 88-89 -1.796384394849003 2316 90-91 -1.6518498752745714 2316 92-93 -2.4123254896822637 2316 94-95 -2.2521800795791584 2316 96-97 -2.9455006392828516 2316 98-99 -4.766372916528194 2316 100-101 -5.228271438473312 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 609.0 3 177.0 4 190.0 5 198.0 6 244.0 7 297.0 8 353.0 9 536.0 10 725.0 11 1172.0 12 1854.0 13 2409.0 14 2437.0 15 2914.0 16 3691.0 17 5085.0 18 3808.0 19 2446.0 20 2290.0 21 2440.0 22 2572.0 23 2766.0 24 3069.0 25 3565.0 26 3997.0 27 4607.0 28 5512.0 29 6380.0 30 7689.0 31 9467.0 32 11645.0 33 14967.0 34 19594.0 35 27015.0 36 33859.0 37 42590.0 38 33218.0 39 5153.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.366651565544394 17.130197456381698 13.091936369027229 18.411214609046677 2 14.312863935531157 22.14127604406451 41.44362475185168 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46-47 20.162922289676864 27.984591617088427 29.850795737652398 22.001690355582316 48-49 20.803748987257862 28.02331148265449 29.97440524416292 21.198534285924726 50-51 21.003776683994115 28.6773872196719 30.96751978109596 19.351316315238027 52-53 19.872244595069873 29.508036731980653 30.479736460744398 20.13998221220508 54-55 18.556928833433997 28.627380934843778 31.281948352312067 21.53374187941016 56-57 20.586063589041046 29.391011653878518 28.978181891835657 21.04474286524478 58-59 20.736935145287813 28.32744079843848 29.445549294737226 21.49007476153648 60-61 20.50232472494591 27.540763246328776 31.332872991759885 20.624039036965428 62-63 21.98610159754292 27.528522291210937 30.156476714467743 20.3288993967784 64-65 20.722945253404628 29.089054363597526 30.793947161028495 19.394053221969347 66-67 19.91775367723567 28.617521450157735 30.400937011397726 21.06378786120887 68-69 19.016374116039046 30.39149408052403 29.867794992898837 20.724336810538084 70-71 20.182708798871104 29.798777151636514 28.24912357621433 21.769390473278055 72-73 19.814590415716722 28.826512760260325 28.96868220708858 22.39021461693437 74-75 19.72365626847556 29.999244861837344 29.135072115694534 21.142026753992564 76-77 20.77227686300512 28.07768814233617 29.727041662062103 21.422993332596604 78-79 19.802501888391458 28.344295215460857 30.661581827225998 21.19162106892168 80-81 19.967641058999785 30.051062998582918 29.430419984410218 20.550875958007087 82-83 19.62558628795443 29.625365221011673 29.033090036881337 21.71595845415256 84-85 19.909758927420395 28.828707711007567 28.83275935100094 22.428774010571097 86-87 20.619847800623237 28.922117530922403 29.39580678193351 21.06222788652085 88-89 21.120638849599246 27.62516206977841 29.16243075200377 22.091768328618578 90-91 19.716636551011497 27.931671538587636 30.509829491975605 21.841862418425258 92-93 19.826212835865427 29.83076194878841 29.360379989060025 20.982645226286138 94-95 19.25546020404405 29.993186254649924 28.737247246878567 22.01410629442746 96-97 18.61064301960459 29.04831625183016 28.73670543385176 23.604335294713483 98-99 19.912416612472068 29.585093506412157 27.822371899623615 22.68011798149216 100-101 20.63397592048675 29.101974932836068 28.462213148283954 21.801835998393226 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 444.0 1 314.0 2 706.0 3 1002.0 4 614.0 5 495.0 6 684.0 7 1014.0 8 1316.5 9 1382.5 10 1181.5 11 975.0 12 947.5 13 982.5 14 1009.5 15 1005.5 16 972.0 17 987.5 18 1055.0 19 1108.5 20 1153.0 21 1240.5 22 1374.0 23 1527.0 24 1686.5 25 1900.0 26 2244.0 27 2634.0 28 2962.5 29 3282.5 30 3696.5 31 4154.0 32 4615.5 33 5136.0 34 5623.0 35 5957.5 36 6422.5 37 6876.5 38 6848.5 39 6644.5 40 6669.0 41 6768.0 42 6846.0 43 7096.0 44 7592.0 45 7984.0 46 8727.0 47 12092.5 48 22489.0 49 22298.0 50 12514.5 51 9056.0 52 6746.5 53 5688.5 54 5092.5 55 4521.0 56 4032.5 57 3567.5 58 3216.5 59 2917.0 60 2481.0 61 2199.0 62 1892.0 63 1349.0 64 887.0 65 672.0 66 516.5 67 362.5 68 284.0 69 220.5 70 164.5 71 127.0 72 108.5 73 114.5 74 90.0 75 61.0 76 47.5 77 31.0 78 26.5 79 17.5 80 7.5 81 5.0 82 3.0 83 3.0 84 2.5 85 1.0 86 0.5 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01473063198093856 2 0.011784505584750849 3 0.013257568782844707 4 0.02099115057283745 5 0.03609004835329948 6 0.048979351336620715 7 0.006260518591898889 8 0.027988200763783267 9 0.032407390358064836 10-11 0.04419189594281568 12-13 0.03572178255377601 14-15 0.029645396861638855 16-17 0.032775656157588304 18-19 0.02522620726735729 20-21 0.054135072529949216 22-23 0.013441701682606437 24-25 0.00368265799523464 26-27 0.0011047973985703922 28-29 0.0 30-31 0.00184132899761732 32-33 9.2066449880866E-4 34-35 0.0 36-37 5.523986992851961E-4 38-39 0.0 40-41 9.2066449880866E-4 42-43 0.00276199349642598 44-45 0.0012889302983321242 46-47 9.2066449880866E-4 48-49 0.0011047973985703922 50-51 0.0031302592959494445 52-53 0.0031302592959494445 54-55 0.0040509237947581045 56-57 9.2066449880866E-4 58-59 0.004787455393805032 60-61 0.0020254618973790522 62-63 3.6826579952346403E-4 64-65 3.6826579952346403E-4 66-67 0.015283030680223757 68-69 0.03995683924829585 70-71 0.04695388943924166 72-73 0.013257568782844707 74-75 0.02559447306688075 76-77 0.026883403365212874 78-79 0.05450333832947268 80-81 0.07788821659921265 82-83 0.048611085537097254 84-85 0.017676758377126275 86-87 0.02099115057283745 88-89 0.020254618973790522 90-91 0.03295978905735003 92-93 0.02264834667069304 94-95 0.012152771384274313 96-97 0.018597422875934935 98-99 0.05321440803114055 100-101 0.0707070335085051 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 271543.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.2812024168686 #Duplication Level Percentage of deduplicated Percentage of total 1 90.51664327061398 59.09035311444437 2 6.025561543144734 7.867118055466609 3 1.3693485306101978 2.681781558180177 4 0.5990697471053203 1.5643197369041877 5 0.36373585905129246 1.1872557120500502 6 0.22519557468808749 0.8820622736757652 7 0.14138323335384215 0.6460767232446475 8 0.1077390195214479 0.5626666193259677 9 0.0912712440607467 0.5362466902532118 >10 0.4705647061872281 5.449809932256533 >50 0.03770847296495592 1.703317919422601 >100 0.03770847296495592 5.413067926413542 >500 0.006753756351932405 3.0142407702638088 >1k 0.007316569381260105 9.401682968098562 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3530 1.2999782723178281 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2825 1.040350883653786 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2671 0.9836379505271725 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2387 0.8790504634625086 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2342 0.8624785024839527 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2176 0.8013463797630578 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2005 0.7383729280445455 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1746 0.6429920859679682 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1327 0.4886887159676368 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1206 0.4441285542252977 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1198 0.44118242782910994 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1097 0.40398758207724006 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1079 0.3973587976858177 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 984 0.3623735467310886 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 917 0.33769973816301657 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 810 0.2982952976140059 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 788 0.2901934500244897 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 668 0.24600155408167398 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 666 0.24526502248262708 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 628 0.23127092210073544 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 584 0.215067226921703 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 584 0.215067226921703 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 540 0.1988635317426706 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 522 0.19223474735124824 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 513 0.18892035515553707 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 495 0.1822915707641147 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 493 0.18155503916506777 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 491 0.18081850756602086 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 467 0.1719801283774577 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 444 0.16351001498841805 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 439 0.16166868599080073 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 406 0.1495159146065264 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 394 0.14509672501224485 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 392 0.14436019341319792 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 364 0.1340487510265409 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 361 0.13294395362797054 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 352 0.12962956143225934 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 351 0.1292612956327359 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 294 0.10827014505989843 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 288 0.10606055026275765 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.8413289976173202E-4 0.0 20-21 0.0 0.0 0.0 3.6826579952346403E-4 0.0 22-23 0.0 0.0 0.0 3.6826579952346403E-4 0.0 24-25 0.0 0.0 0.0 5.523986992851961E-4 0.0 26-27 0.0 0.0 0.0 0.0025778605966642484 0.0 28-29 0.0 0.0 0.0 0.00552398699285196 0.0 30-31 0.0 0.0 0.0 0.009574910787610066 0.0 32-33 0.0 0.0 0.0 0.015098897780462027 0.0 34-35 0.0 0.0 0.0 0.019518087374743594 0.0 36-37 0.0 0.0 0.0 0.02522620726735729 0.0 38-39 0.0 0.0 0.0 0.031670858759017914 0.0 40-41 0.0 0.0 0.0 0.038115510250678525 0.0 42-43 0.0 0.0 0.0 0.05910666082351598 0.0 44-45 0.0 0.0 0.0 0.07825648239873612 0.0 46-47 0.0 0.0 0.0 0.09188231698110429 0.0 48-49 0.0 0.0 0.0 0.1137941320527504 0.0 50-51 0.0 0.0 0.0 0.13975687091915462 0.0 52-53 0.0 0.0 0.0 0.19444434214838902 0.0 54-55 0.0 0.0 0.0 0.24250302898620107 0.0 56-57 0.0 0.0 0.0 0.3106322018980419 0.0 58-59 0.0 0.0 0.0 0.4257152642491244 0.0 60-61 0.0 0.0 0.0 0.5382204660035428 0.0 62-63 0.0 0.0 0.0 0.6825806594167407 0.0 64-65 0.0 0.0 0.0 0.7965589243692528 0.0 66-67 0.0 0.0 0.0 0.9471796363743495 0.0 68-69 0.0 0.0 0.0 1.1373889218282187 0.0 70-71 0.0 0.0 0.0 1.2889302983321242 0.0 72-73 0.0 0.0 0.0 1.5078643161488237 0.0 74-75 0.0 0.0 0.0 1.7262459352662378 0.0 76-77 0.0 0.0 0.0 1.8788921091687136 0.0 78-79 0.0 0.0 0.0 2.0536342310425972 0.0 80-81 0.0 0.0 0.0 2.3445642126661337 0.0 82-83 0.0 0.0 0.0 2.587803773251382 0.0 84-85 0.0 0.0 0.0 2.983505374839344 0.0 86-87 0.0 0.0 0.0 3.4690638315110314 0.0 88-89 0.0 0.0 0.0 4.028827846786697 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 695 0.0 60.825333 2 ACCTGGG 705 0.0 58.61509 3 TAACGCA 50 4.2308056E-7 56.99812 4 TATAACG 50 4.2308056E-7 56.99812 2 GTACCTG 790 0.0 53.51089 1 ATAACGC 55 8.169245E-7 51.816475 3 CCTGGGA 510 0.0 42.841724 4 GTGTATC 45 9.613624E-4 42.220833 1 GTAAGCG 30 8.282083E-5 39.585674 94-95 ATGGGTA 105 4.636422E-8 36.18928 5 CCTGGGG 370 0.0 35.94476 4 GTACCAG 110 6.9810994E-8 34.544315 1 TGGGGAG 290 0.0 29.481785 6 TCACGCA 65 0.0058372538 29.229805 4 TTCAACG 65 0.0058372538 29.229805 2 GTCTTAG 65 0.0058372538 29.229805 1 TGGGAGT 165 2.6302587E-9 28.786928 6 CATGGGT 215 1.8189894E-12 28.719984 4 GAGTAGG 85 6.3957146E-4 27.940254 1 ATGGGAG 445 0.0 25.617134 5 >>END_MODULE