##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936735_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 271543 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77857650537852 31.0 31.0 34.0 30.0 34.0 2 31.823703796452126 31.0 31.0 34.0 30.0 34.0 3 31.931778760638277 31.0 31.0 34.0 30.0 34.0 4 35.68672364966138 37.0 35.0 37.0 33.0 37.0 5 35.570753066733445 37.0 35.0 37.0 33.0 37.0 6 35.67854814891196 37.0 35.0 37.0 33.0 37.0 7 35.621765245283434 37.0 35.0 37.0 33.0 37.0 8 35.622115097792985 37.0 35.0 37.0 33.0 37.0 9 37.174642690108016 39.0 37.0 39.0 33.0 39.0 10-11 37.04366527584949 39.0 37.0 39.0 33.0 39.0 12-13 36.913671131275706 39.0 37.0 39.0 33.0 39.0 14-15 37.77695797718962 39.0 37.0 41.0 32.5 41.0 16-17 37.80067797733692 39.0 37.0 41.0 32.5 41.0 18-19 38.10829776499486 40.0 37.0 41.0 33.0 41.0 20-21 38.127974206663396 40.0 37.0 41.0 33.0 41.0 22-23 37.985849386653314 40.0 37.0 41.0 33.0 41.0 24-25 37.825743988981486 40.0 36.5 41.0 33.0 41.0 26-27 37.45421903713224 39.0 36.0 41.0 32.0 41.0 28-29 37.38753714881253 39.0 36.0 41.0 32.0 41.0 30-31 37.16699012679392 39.0 36.0 41.0 32.0 41.0 32-33 36.799109165030956 39.0 35.0 41.0 31.0 41.0 34-35 36.47165274008168 39.0 35.0 40.0 30.5 41.0 36-37 36.12555470036053 38.0 35.0 40.0 30.0 41.0 38-39 35.822127618830166 38.0 35.0 40.0 30.0 41.0 40-41 35.54599823968948 38.0 35.0 40.0 29.0 41.0 42-43 35.26030499773516 38.0 35.0 40.0 27.5 41.0 44-45 34.8004625418442 38.0 34.0 40.0 25.0 41.0 46-47 34.153572362388275 38.0 33.5 40.0 22.0 41.0 48-49 33.53404249050794 38.0 33.0 40.0 16.5 41.0 50-51 33.14888618008934 38.0 33.0 40.0 12.0 41.0 52-53 32.77891530991408 37.0 32.5 40.0 8.5 41.0 54-55 32.500565288002264 37.0 32.0 40.0 7.5 41.0 56-57 32.19141167328931 37.0 31.5 40.0 3.5 41.0 58-59 31.860204461171897 36.0 31.0 39.0 2.0 40.5 60-61 31.38589100068866 36.0 30.5 39.0 2.0 40.0 62-63 31.016428337316743 35.0 30.0 39.0 2.0 40.0 64-65 30.62050209359107 35.0 29.5 38.5 2.0 40.0 66-67 30.18810464640959 35.0 29.0 38.0 2.0 40.0 68-69 29.7635991353119 34.5 28.5 37.5 2.0 39.5 70-71 29.196495582651735 34.0 28.0 37.0 2.0 39.0 72-73 28.582097494687766 34.0 26.5 36.0 2.0 39.0 74-75 28.05561550104403 33.0 26.0 35.5 2.0 38.0 76-77 26.65262039529651 31.5 25.0 34.0 2.0 36.0 78-79 27.36378952873026 33.0 26.0 35.0 2.0 37.0 80-81 27.19809938020866 33.0 26.0 35.0 2.0 36.5 82-83 26.785337865457773 33.0 25.0 35.0 2.0 36.0 84-85 26.353378654577725 32.5 24.5 35.0 2.0 36.0 86-87 25.859959564415213 32.0 23.0 35.0 2.0 35.0 88-89 25.217503305185552 32.0 20.0 34.0 2.0 35.0 90-91 24.530553172057466 31.0 17.5 34.0 2.0 35.0 92-93 23.980544517811175 31.0 9.0 34.0 2.0 35.0 94-95 23.27132166912791 31.0 2.0 34.0 2.0 35.0 96-97 22.35025207793977 30.0 2.0 34.0 2.0 35.0 98-99 21.214704485109174 29.0 2.0 34.0 2.0 35.0 100-101 17.797722644295746 22.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1686210354657014 1101 2 0.22013056115067187 1101 3 0.14609735534891044 1101 4 0.05809343255862842 1101 5 0.07413151256533013 1101 6 0.13481755461226896 1101 7 0.2966761539049898 1101 8 0.2488645132071028 1101 9 0.31466025541636355 1101 10-11 0.31449485930806276 1101 12-13 0.39716527189757755 1101 14-15 0.6300904798450873 1101 16-17 0.29236229950228676 1101 18-19 0.2789196286259852 1101 20-21 0.394318549398065 1101 22-23 0.2024086927551778 1101 24-25 0.3830025960446761 1101 26-27 0.338396500454742 1101 28-29 0.3297787169276347 1101 30-31 0.8089635913444368 1101 32-33 0.7035273850099557 1101 34-35 0.5713317910111613 1101 36-37 0.5143912660970358 1101 38-39 0.3364964985965031 1101 40-41 0.3188988641450692 1101 42-43 0.8330568963286709 1101 44-45 0.8180491520685536 1101 46-47 1.1618733369235628 1101 48-49 0.8628581037387661 1101 50-51 1.0169790686987952 1101 52-53 1.007319379639732 1101 54-55 1.0556930006144896 1101 56-57 1.1390104749492025 1101 58-59 1.0857380350122092 1101 60-61 1.2321891803168192 1101 62-63 1.1530458195311653 1101 64-65 1.2662672994605195 1101 66-67 1.1909806631747948 1101 68-69 1.0572544638767845 1101 70-71 0.7768264359951722 1101 72-73 1.0152299492212116 1101 74-75 1.38763441463745 1101 76-77 1.4058016113254368 1101 78-79 1.541671518637493 1101 80-81 1.344601691500845 1101 82-83 1.1781045011334221 1101 84-85 1.0889552364389168 1101 86-87 1.0514839925143384 1101 88-89 1.2125159107990058 1101 90-91 1.5964618510575903 1101 92-93 1.7776561158295259 1101 94-95 1.1487667741766252 1101 96-97 1.4324612890288009 1101 98-99 0.8835476273271148 1101 100-101 1.192468248635425 1102 1 0.2433244006541848 1102 2 0.4559888708313373 1102 3 0.3223813262192401 1102 4 0.16530293876040503 1102 5 0.2929555627697411 1102 6 0.16471105251608265 1102 7 0.14312044211163766 1102 8 0.14778734041678376 1102 9 0.49845232027240627 1102 10-11 0.33741624565808337 1102 12-13 0.31697839413629936 1102 14-15 0.43897476839892846 1102 16-17 0.4627331038665332 1102 18-19 0.07750090265839304 1102 20-21 0.25519964375700965 1102 22-23 0.2767865625396553 1102 24-25 0.44063481227778567 1102 26-27 0.5143267663193853 1102 28-29 0.34449327915801575 1102 30-31 0.7108397468531251 1102 32-33 0.7129971312971364 1102 34-35 0.8480896831897198 1102 36-37 0.7839253302760483 1102 38-39 0.762020980190826 1102 40-41 1.0032030417700781 1102 42-43 0.7048355768137 1102 44-45 0.6407756751700475 1102 46-47 1.096355452036093 1102 48-49 1.4300468650334537 1102 50-51 1.1340424057644753 1102 52-53 1.256776887299722 1102 54-55 1.2998731427908865 1102 56-57 1.4063940994924309 1102 58-59 1.5330707086748596 1102 60-61 1.2406368710354165 1102 62-63 1.4150218804291796 1102 64-65 1.3640222491606302 1102 66-67 1.0552165301643512 1102 68-69 0.8856402670692418 1102 70-71 1.0273298688866994 1102 72-73 0.9905639075057202 1102 74-75 0.5849844480549784 1102 76-77 0.6955489400931505 1102 78-79 1.1988771188944156 1102 80-81 1.5968497797578358 1102 82-83 1.6231652168761173 1102 84-85 0.9162473750739188 1102 86-87 1.3221681404541705 1102 88-89 1.3971412438893047 1102 90-91 1.7439763681526088 1102 92-93 1.881029116202761 1102 94-95 1.6690928771720515 1102 96-97 1.5181251694548088 1102 98-99 1.4155872249279966 1102 100-101 1.3728838411672584 1103 1 0.039002834011835574 1103 2 0.18136988631017203 1103 3 0.26426250795035244 1103 4 0.2307467827115488 1103 5 0.3002657001884188 1103 6 0.28957891917679035 1103 7 0.20320947392785627 1103 8 0.109231700494405 1103 9 0.050355288495893546 1103 10-11 0.22993787086093675 1103 12-13 0.17447289788565712 1103 14-15 0.3789651406551471 1103 16-17 0.365393636568605 1103 18-19 0.40963821215066076 1103 20-21 0.34431582680325334 1103 22-23 0.2605633179900053 1103 24-25 0.17849715539270505 1103 26-27 0.03511078227960951 1103 28-29 0.18733028741107915 1103 30-31 0.5063494465568539 1103 32-33 0.4944547915568904 1103 34-35 0.20964868289691907 1103 36-37 0.24883617439095929 1103 38-39 0.34856893776919406 1103 40-41 0.638177558025582 1103 42-43 0.5577866787933488 1103 44-45 0.10984778895608827 1103 46-47 0.4382371300426584 1103 48-49 0.47809873574377804 1103 50-51 0.7249104411588405 1103 52-53 0.7711093223744925 1103 54-55 0.48963150627294993 1103 56-57 0.67743990075396 1103 58-59 0.77101741861674 1103 60-61 0.6744089118689693 1103 62-63 0.8895803193451179 1103 64-65 0.5856390153305249 1103 66-67 0.8406394312913896 1103 68-69 1.118462932961716 1103 70-71 0.8809906447637452 1103 72-73 0.8816934800636709 1103 74-75 1.262390515518664 1103 76-77 1.2330847339601796 1103 78-79 1.0109152725512232 1103 80-81 1.483488059468609 1103 82-83 1.217455071517037 1103 84-85 1.6749756195987615 1103 86-87 1.7381065228939612 1103 88-89 1.2435489540247353 1103 90-91 1.0777712376569824 1103 92-93 1.2416724876996774 1103 94-95 1.536130757877359 1103 96-97 1.760513544698604 1103 98-99 1.3650589397085682 1103 100-101 0.788780040193565 1104 1 0.3734208836918427 1104 2 0.4100660572614636 1104 3 0.40999414912618093 1104 4 0.27166770688987896 1104 5 0.1444028083346396 1104 6 0.2381470935991814 1104 7 0.11226143184086368 1104 8 0.008369350998798097 1104 9 -0.0015321179473204438 1104 10-11 0.2096618105507062 1104 12-13 0.3390216558854391 1104 14-15 0.500233716422585 1104 16-17 0.2660010777227413 1104 18-19 0.135896483112802 1104 20-21 0.12052799732066433 1104 22-23 0.20074392326171875 1104 24-25 0.02074685709571611 1104 26-27 0.3819271398021087 1104 28-29 0.22041142419380577 1104 30-31 0.27559231454686994 1104 32-33 0.5885729171670491 1104 34-35 0.6928220205691176 1104 36-37 0.5231799210012227 1104 38-39 0.636102835155036 1104 40-41 0.5559886004379919 1104 42-43 0.7432731740748295 1104 44-45 0.9720237299475158 1104 46-47 0.8872907150301899 1104 48-49 0.6907844934181426 1104 50-51 0.9442509336200615 1104 52-53 1.0338466040753218 1104 54-55 0.5239627786452274 1104 56-57 1.127176857039892 1104 58-59 0.9232356635456931 1104 60-61 0.5979120033105545 1104 62-63 0.7520355747958192 1104 64-65 0.800724707921912 1104 66-67 0.7770886123305516 1104 68-69 0.7454208459674696 1104 70-71 0.7204282193379932 1104 72-73 0.9850316946205666 1104 74-75 1.0075879338937597 1104 76-77 0.8894954075690329 1104 78-79 1.1520205985084857 1104 80-81 1.166091921058804 1104 82-83 1.1118362408412565 1104 84-85 1.059781455372704 1104 86-87 1.0423206459007979 1104 88-89 1.0625396254641544 1104 90-91 0.860036125388838 1104 92-93 1.3065737785121314 1104 94-95 1.4376113956905705 1104 96-97 1.7691289042820735 1104 98-99 1.2211116569230178 1104 100-101 0.8880098915874264 1105 1 0.12394114320953165 1105 2 0.19647198209926842 1105 3 0.258629232979775 1105 4 0.02919616814584458 1105 5 0.22285794360210787 1105 6 0.15021099370935787 1105 7 0.04884485698470087 1105 8 -0.17545363835118621 1105 9 -0.10951963171889645 1105 10-11 -0.05497584399697786 1105 12-13 0.034305656375096305 1105 14-15 0.28934805110247197 1105 16-17 0.32375600488640544 1105 18-19 0.22230546830071063 1105 20-21 -0.014910856884263524 1105 22-23 -0.054645353271510544 1105 24-25 0.26255123951706594 1105 26-27 0.245521214964306 1105 28-29 -0.19851684119975488 1105 30-31 -0.04417142642509475 1105 32-33 -0.051887971558620904 1105 34-35 -0.21930002619303224 1105 36-37 0.15753847161941792 1105 38-39 0.23947840131980058 1105 40-41 0.07296742281526747 1105 42-43 -0.03215833528984291 1105 44-45 -0.2127479679347175 1105 46-47 -0.18371858843413236 1105 48-49 0.11277616926586376 1105 50-51 0.3862756612489022 1105 52-53 0.08588173689451395 1105 54-55 -0.10420468371246727 1105 56-57 0.24305701372981758 1105 58-59 0.30005045115740003 1105 60-61 0.3685050991607177 1105 62-63 0.23548214108300058 1105 64-65 0.14169361476485776 1105 66-67 0.5335000457985366 1105 68-69 0.445362699315492 1105 70-71 0.4499514167917802 1105 72-73 0.7616965250771734 1105 74-75 0.5305742235252957 1105 76-77 0.48327065545739245 1105 78-79 0.2977116255619805 1105 80-81 0.7936870979470392 1105 82-83 0.09906846020715676 1105 84-85 0.3045145928436348 1105 86-87 0.23370882070799226 1105 88-89 0.7377722120720627 1105 90-91 1.014865357853033 1105 92-93 1.067734875341607 1105 94-95 0.49223864504789816 1105 96-97 0.7910807955784378 1105 98-99 0.5932563502759329 1105 100-101 0.6563658715115288 1106 1 0.026737701748544396 1106 2 -0.031153320663111828 1106 3 -0.07734413879013857 1106 4 0.11291461027551009 1106 5 -0.09264646224064421 1106 6 0.024150396654505357 1106 7 -0.25791473110986374 1106 8 -0.04435566964529869 1106 9 0.1942977747033794 1106 10-11 -0.05747967031910406 1106 12-13 0.08514744724244139 1106 14-15 0.2160126869703376 1106 16-17 0.07679105845491563 1106 18-19 0.05500603444778562 1106 20-21 -0.21997571861658116 1106 22-23 -0.395436919017385 1106 24-25 -0.10226442834331806 1106 26-27 0.05840663333362528 1106 28-29 0.04870071678946175 1106 30-31 0.036107040668532875 1106 32-33 -0.29939157498819924 1106 34-35 -0.04723440662603906 1106 36-37 0.041560047618347085 1106 38-39 -0.09681073489726799 1106 40-41 0.1510615429097797 1106 42-43 0.21189414847615495 1106 44-45 0.461550292017435 1106 46-47 0.3511566660348677 1106 48-49 0.2517533896471562 1106 50-51 0.23052949128589972 1106 52-53 0.21559034203293237 1106 54-55 0.3472493186947716 1106 56-57 0.3794821144029541 1106 58-59 0.38831961620190114 1106 60-61 0.16254866722467298 1106 62-63 0.30135431963479675 1106 64-65 0.6879735380898175 1106 66-67 0.6083919429113358 1106 68-69 0.3483041380126082 1106 70-71 0.350024971333589 1106 72-73 0.7003564950609729 1106 74-75 1.1873085504640848 1106 76-77 0.9061758369662236 1106 78-79 0.37189398138764673 1106 80-81 0.5728563031122 1106 82-83 0.6204104219587947 1106 84-85 0.5140410204704224 1106 86-87 0.5504131314499254 1106 88-89 0.3514399832951476 1106 90-91 0.5530171328207132 1106 92-93 0.1484331234061571 1106 94-95 -0.27048108985856345 1106 96-97 -0.30938059200198964 1106 98-99 -0.4321088220200089 1106 100-101 0.0964189199386496 1107 1 0.05266350061992142 1107 2 0.15627914190185166 1107 3 -0.03885423295661994 1107 4 -0.07222801163491965 1107 5 0.010687957409103888 1107 6 -0.011895297244393532 1107 7 0.005768567632870258 1107 8 0.028653369650768923 1107 9 0.005398784977643345 1107 10-11 0.11778318966614876 1107 12-13 -0.057251482450013214 1107 14-15 0.006037729422445182 1107 16-17 0.012984262688114256 1107 18-19 -0.20875873802609846 1107 20-21 0.088565301263003 1107 22-23 -0.06696485164290067 1107 24-25 -0.023989613160040335 1107 26-27 0.008265983945108246 1107 28-29 0.4299034405849298 1107 30-31 0.33070747633858844 1107 32-33 0.00710831352143515 1107 34-35 -0.09799682922599828 1107 36-37 0.5108830437933705 1107 38-39 0.5979758039271061 1107 40-41 0.6729308075879104 1107 42-43 0.6059268408827947 1107 44-45 0.3380404036793294 1107 46-47 0.8232840737980212 1107 48-49 0.06188100323868895 1107 50-51 -0.028166757491995043 1107 52-53 0.03327786025732138 1107 54-55 0.13311920085802598 1107 56-57 -0.18579120852461273 1107 58-59 -0.2566993371608213 1107 60-61 -0.30343390597304065 1107 62-63 -0.33655069057085996 1107 64-65 -0.051207608031173635 1107 66-67 0.2366230195652541 1107 68-69 0.23690381897862878 1107 70-71 -0.29353603105058923 1107 72-73 0.020848424873499027 1107 74-75 0.22838417523896126 1107 76-77 0.14174212227865368 1107 78-79 0.5437049280266422 1107 80-81 0.2858071958927688 1107 82-83 0.571084509881814 1107 84-85 0.31609415849549904 1107 86-87 0.5107182720135519 1107 88-89 0.5478688610159423 1107 90-91 0.6219267570124067 1107 92-93 0.6726222453766191 1107 94-95 1.0392434431683135 1107 96-97 1.2577151696487512 1107 98-99 1.4884071211027532 1107 100-101 1.294481560373633 1108 1 0.2087383440093049 1108 2 0.15887558107099053 1108 3 0.09600605388808958 1108 4 -0.09341165818948838 1108 5 0.09708137976321041 1108 6 -0.21641639846431104 1108 7 -0.12791713958770856 1108 8 0.22186141449085994 1108 9 -0.1005400544552586 1108 10-11 -0.05586398310008889 1108 12-13 -0.3050259631621799 1108 14-15 -0.23409168040281259 1108 16-17 -0.20761003338837014 1108 18-19 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sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 13.0 6 62.0 7 126.0 8 235.0 9 412.0 10 693.0 11 1199.0 12 1832.0 13 2461.0 14 2530.0 15 3238.0 16 4311.0 17 5506.0 18 4720.0 19 2833.0 20 2433.0 21 2477.0 22 2745.0 23 2953.0 24 3381.0 25 3695.0 26 4527.0 27 5485.0 28 6491.0 29 7849.0 30 9236.0 31 11268.0 32 13543.0 33 17348.0 34 22262.0 35 28875.0 36 36702.0 37 40368.0 38 19018.0 39 711.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.827587730929025 17.88834379327617 13.712837738295178 17.571230737499633 2 14.689330043092335 23.779234650657436 40.39482891974513 21.136606386505104 3 22.06831783445355 31.396372341405026 29.773317374090784 16.761992450050638 4 11.553971194249161 17.38177747170798 38.836942951945 32.22730838209786 5 11.15019021909262 41.133359604314826 32.595413381259455 15.121036795333096 6 24.581373852391703 37.19300442287225 20.86594020099947 17.359681523736572 7 25.780542236560088 35.97270403841763 21.919997643090202 16.326756081932075 8 25.28669598073226 30.56912006245811 21.35875849776462 22.78542545904501 9 27.252773962134913 19.165288738800115 22.89066556677948 30.691271732285497 10-11 24.037445266495546 26.781209605845113 27.6026264716822 21.578718655977138 12-13 29.93172352076835 24.868805308919768 27.24743410804182 17.952037062270065 14-15 24.4848355395808 25.599958753698814 31.19067391668616 18.72453179003423 16-17 18.802054273629395 28.69888024041409 32.03109031548826 20.467975170468254 18-19 18.214292948171444 29.168868617641262 34.53972626450296 18.077112169684337 20-21 20.839946822026874 25.90308645839855 33.2874225255118 19.969544194062774 22-23 19.41114190228049 26.36419713304547 32.918603901783335 21.30605706289071 24-25 20.929543127766607 26.1865567757474 33.93195159496506 18.951948501520942 26-27 21.567197707905223 24.06607449308026 36.10620088236811 18.26052691664641 28-29 18.773488496275906 26.54427944060322 36.10263586731359 18.579596195807284 30-31 19.1947779332695 27.518781763276127 34.742763497090664 18.543676806363703 32-33 19.337648347803672 27.01720788487566 34.164786903961776 19.480356863358896 34-35 20.563599168448167 25.573533229849694 34.09878084484635 19.764086756855793 36-37 21.47186960252487 25.042995665447688 35.57058102151072 17.91455371051672 38-39 21.778601101912145 25.054690492324916 35.218467340384784 17.948241065378156 40-41 19.328062222189487 26.796124370725817 35.5904957962459 18.2853176108388 42-43 18.93603348303476 28.29817283241358 32.89012136311158 19.87567232144008 44-45 19.764337168363213 28.021190872530678 31.702524932604632 20.51194702650148 46-47 20.732996247371503 27.32845258393698 30.769712347583994 21.16883882110752 48-49 21.418733289631653 27.571831566852524 30.882970339326356 20.12646480418947 50-51 21.373514475007088 28.455054007372844 31.615949207658623 18.55548230996144 52-53 20.129666865530933 29.08997503148684 31.334747479211316 19.44561062377091 54-55 18.680098547927955 28.37432745458362 31.740092729328317 21.205481268160106 56-57 21.19308105798748 28.87091160541479 29.699225274431846 20.23678206216588 58-59 21.372633145083626 27.78558315364601 30.05176099714035 20.790022704130013 60-61 20.648681295815834 27.366403287967874 32.0492164008021 19.93569901541419 62-63 22.354102296873794 27.15058020276715 30.980360384911414 19.514957115447647 64-65 20.880956195253273 28.749201298545856 31.3768600306775 18.99298247552337 66-67 20.206636603514404 27.90883094356438 31.183492641033812 20.701039811887405 68-69 19.65279069296393 30.02966452023945 30.273462315379334 20.044082471417287 70-71 20.790197659901224 29.253444408843322 28.92345880888457 21.03289912237089 72-73 20.26357656868017 28.243608535211063 29.66866027827086 21.824154617837912 74-75 20.218209941902753 30.010219959304308 29.356694994061378 20.414875104731564 76-77 21.305740048869502 27.551608513678488 30.212547345782102 20.930104091669904 78-79 20.12606411481668 27.961521534571876 31.2876917156026 20.624722635008848 80-81 20.304674150020624 29.616699074892466 30.122893465323198 19.955733309763716 82-83 20.026737180148196 28.930475966000323 29.798513619020962 21.24427323483052 84-85 19.987110317270336 28.688014436444657 29.281860533632866 22.043014712652145 86-87 20.69378232712984 28.2889661026624 29.750442383364543 21.266809186843215 88-89 21.32045592649198 27.283775572209844 29.35864621503674 22.03712228626144 90-91 20.101981250034527 27.640822700311762 30.731197091932284 21.525998957721423 92-93 19.776676628568378 29.449296498235032 29.856427083851223 20.917599789345367 94-95 19.40395521838403 29.89080798409074 28.89426972085144 21.810967076673787 96-97 18.748411853739555 28.776473707663243 28.949926899238793 23.52518753935841 98-99 20.11950357221772 29.200670251160048 28.079104367680635 22.600721808941593 100-101 20.448192682342974 29.155173366232713 28.40112692654722 21.995507024877085 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 552.0 1 457.5 2 1191.0 3 1555.5 4 805.0 5 517.0 6 653.5 7 1038.5 8 1371.0 9 1459.0 10 1283.5 11 1003.0 12 916.0 13 932.0 14 975.5 15 997.0 16 983.0 17 1061.0 18 1108.5 19 1165.0 20 1236.5 21 1305.5 22 1475.5 23 1638.0 24 1779.0 25 2024.5 26 2378.5 27 2740.0 28 3052.0 29 3369.5 30 3793.5 31 4203.0 32 4595.0 33 4993.0 34 5549.5 35 5926.0 36 6182.0 37 6549.5 38 6610.0 39 6476.5 40 6502.0 41 6694.5 42 6825.5 43 6941.0 44 7477.5 45 7931.5 46 8592.0 47 12155.0 48 23390.5 49 22852.0 50 12375.0 51 8961.5 52 6379.0 53 5272.5 54 4749.0 55 4262.0 56 3743.5 57 3361.0 58 3097.5 59 2846.5 60 2513.5 61 2182.5 62 1808.0 63 1296.0 64 852.0 65 652.5 66 495.5 67 352.0 68 263.5 69 202.5 70 171.0 71 133.0 72 121.5 73 118.5 74 93.0 75 72.5 76 57.0 77 40.0 78 27.0 79 18.5 80 12.5 81 7.5 82 8.5 83 7.0 84 2.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011416239785227385 2 0.012152771384274313 3 0.006628784391422353 4 0.0 5 0.005155721193328497 6 0.0 7 3.6826579952346403E-4 8 3.6826579952346403E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012889302983321242 16-17 0.004235056694519837 18-19 0.0012889302983321242 20-21 7.365315990469281E-4 22-23 1.8413289976173202E-4 24-25 3.6826579952346403E-4 26-27 3.6826579952346403E-4 28-29 1.8413289976173202E-4 30-31 0.0011047973985703922 32-33 0.0038667908949963723 34-35 9.2066449880866E-4 36-37 0.0014730631980938561 38-39 0.00552398699285196 40-41 0.0 42-43 9.2066449880866E-4 44-45 0.0040509237947581045 46-47 0.0 48-49 0.00184132899761732 50-51 0.0022095947971407844 52-53 3.6826579952346403E-4 54-55 0.0 56-57 0.010127309486895261 58-59 0.002393727696902516 60-61 0.00276199349642598 62-63 0.0 64-65 0.0031302592959494445 66-67 0.0012889302983321242 68-69 0.002393727696902516 70-71 0.0058922527923754246 72-73 0.0033143921957111766 74-75 0.005708119892613692 76-77 0.0012889302983321242 78-79 0.005339854093090229 80-81 0.0025778605966642484 82-83 0.0040509237947581045 84-85 0.0029461263961877123 86-87 9.2066449880866E-4 88-89 0.0029461263961877123 90-91 0.008285980489277941 92-93 0.0031302592959494445 94-95 0.0011047973985703922 96-97 0.0 98-99 0.0011047973985703922 100-101 0.0029461263961877123 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 271543.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.283324634857514 #Duplication Level Percentage of deduplicated Percentage of total 1 90.03102935429702 56.074318284840686 2 6.242606447447434 7.776205678680463 3 1.4507613548006402 2.7107472128626195 4 0.6445859680184056 1.6058782840465702 5 0.37714184470882994 1.174482397369454 6 0.2518473440966804 0.9411533934480129 7 0.1709553683129941 0.7453368091896893 8 0.13167548614026345 0.6560949639781368 9 0.09735060981140344 0.5456987670856488 >10 0.5012187079893136 5.66786986923925 >50 0.04186405567701966 1.8307114595442078 >100 0.040095151916018826 4.975054792867679 >500 0.011792691740005537 5.220741044910823 >1k 0.007075615044003323 10.075707041936786 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4200 1.546716357998549 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3301 1.2156454042269549 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2935 1.080860121601367 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2914 1.0731265398113743 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2743 1.010153088092862 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2617 0.9637515973529054 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2026 0.7461065098345382 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1821 0.670612020932228 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1669 0.6146356194046615 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1090 0.40140972148057585 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1074 0.3955174686882004 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1046 0.38520602630154344 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 984 0.3623735467310886 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 965 0.3553764965401428 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 919 0.3384362697620635 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 915 0.3369632065639696 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 810 0.2982952976140059 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 806 0.29682223441591205 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 786 0.28945691842544274 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 720 0.2651513756568941 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 689 0.25373513587166674 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 689 0.25373513587166674 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 679 0.2500524778764321 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 664 0.2445284908835801 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 630 0.23200745369978235 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 626 0.23053439050168847 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 613 0.22574693510788346 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 581 0.2139624295231326 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 554 0.2040192529359991 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 545 0.20070486074028793 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 534 0.19665393694552982 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 507 0.1867107603583963 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 496 0.18265983656363816 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 426 0.15688123059699569 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 388 0.14288713021510405 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 361 0.13294395362797054 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 360 0.13257568782844706 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 341 0.12557863763750124 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 335 0.12336904284036046 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 334 0.12300077704083699 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 298 0.10974320825799229 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 294 0.10827014505989843 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 286 0.10532401866371072 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 284 0.1045874870646638 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 281 0.1034826896660934 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 280 0.10311442386656992 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 280 0.10311442386656992 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.8413289976173202E-4 0.0 20-21 0.0 0.0 0.0 3.6826579952346403E-4 0.0 22-23 0.0 0.0 0.0 3.6826579952346403E-4 0.0 24-25 0.0 0.0 0.0 5.523986992851961E-4 0.0 26-27 0.0 0.0 0.0 0.0025778605966642484 0.0 28-29 0.0 0.0 0.0 0.006076385692137157 0.0 30-31 0.0 0.0 0.0 0.010495575286418726 0.0 32-33 0.0 0.0 0.0 0.016019562279270688 0.0 34-35 0.0 0.0 0.0 0.02099115057283745 0.0 36-37 0.0 0.0 0.0 0.027251669164736342 0.0 38-39 0.0 0.0 0.0 0.03388045355615869 0.0 40-41 0.0 0.0 0.0 0.03995683924829585 0.0 42-43 0.0 0.0 0.0 0.06057972402160984 0.0 44-45 0.0 0.0 0.0 0.07972954559682996 0.0 46-47 0.0 0.0 0.0 0.09390777887848334 0.0 48-49 0.0 0.0 0.0 0.11692439134869984 0.0 50-51 0.0 0.0 0.0 0.14417606051343618 0.0 52-53 0.0 0.0 0.0 0.19775873434410018 0.0 54-55 0.0 0.0 0.0 0.24416022508405666 0.0 56-57 0.0 0.0 0.0 0.3121052650961358 0.0 58-59 0.0 0.0 0.0 0.42737246034698007 0.0 60-61 0.0 0.0 0.0 0.5396935292016366 0.0 62-63 0.0 0.0 0.0 0.6857109187126901 0.0 64-65 0.0 0.0 0.0 0.799873316564964 0.0 66-67 0.0 0.0 0.0 0.9490209653719669 0.0 68-69 0.0 0.0 0.0 1.138677852126551 0.0 70-71 0.0 0.0 0.0 1.291139893129265 0.0 72-73 0.0 0.0 0.0 1.5095215122466792 0.0 74-75 0.0 0.0 0.0 1.7295603274619489 0.0 76-77 0.0 0.0 0.0 1.8838636974622804 0.0 78-79 0.0 0.0 0.0 2.0575010219375938 0.0 80-81 0.0 0.0 0.0 2.3473262061625597 0.0 82-83 0.0 0.0 0.0 2.588356171950667 0.0 84-85 0.0 0.0 0.0 2.98203231164125 0.0 86-87 0.0 0.0 0.0 3.465933572215082 0.0 88-89 0.0 0.0 0.0 4.019621201798611 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 25 5.260991E-5 76.02063 3 TACCTGG 460 0.0 57.841785 2 GTACCCG 25 0.0050081136 57.025978 1 ACCTGGG 525 0.0 48.870407 3 GTACCTG 585 0.0 47.115482 1 TATAACG 45 9.5992297E-4 42.23369 2 TAACGCA 45 9.616655E-4 42.218132 4 TAAGGGT 50 0.0016114913 38.010315 3 CCTGGGG 225 0.0 35.88541 4 CCATATG 55 0.0025773959 34.542107 4 AAGGGTA 55 0.0025773959 34.542107 4 GTCGGGA 70 2.0459513E-4 33.937782 2 GCTTATA 470 0.0 32.355167 1 CCTGGGA 500 0.0 32.296867 4 GTATTAC 60 0.0039368887 31.6811 1 ATACTCT 485 0.0 29.378597 5 TCGGGAG 85 6.384338E-4 27.948761 3 TGGGAGT 175 4.9312803E-9 27.140228 6 ACGTGAC 35 0.008391707 27.140226 22-23 TATACTC 535 0.0 26.632935 4 >>END_MODULE