##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936734_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 293428 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50735444470194 31.0 31.0 34.0 30.0 34.0 2 31.444115762640237 31.0 31.0 34.0 28.0 34.0 3 31.459785705522307 31.0 31.0 34.0 28.0 34.0 4 35.12396908270513 37.0 35.0 37.0 32.0 37.0 5 35.12259225431792 37.0 35.0 37.0 32.0 37.0 6 35.265932358193496 37.0 35.0 37.0 32.0 37.0 7 35.157650258325724 37.0 35.0 37.0 32.0 37.0 8 35.180054391537276 37.0 35.0 37.0 32.0 37.0 9 36.67655438472129 39.0 37.0 39.0 32.0 39.0 10-11 36.509053328244065 39.0 37.0 39.0 32.0 39.0 12-13 36.422311435854795 39.0 37.0 39.0 32.0 39.0 14-15 37.33596487042818 39.0 37.0 41.0 32.0 41.0 16-17 37.33119197895225 39.0 36.5 41.0 32.0 41.0 18-19 37.44786114481236 39.5 37.0 41.0 32.0 41.0 20-21 37.37377653121038 39.0 37.0 41.0 32.0 41.0 22-23 37.22984684488188 39.0 36.0 41.0 31.5 41.0 24-25 37.04073571710948 39.0 36.0 41.0 31.0 41.0 26-27 36.746333001622205 39.0 35.5 40.5 30.5 41.0 28-29 36.804076298103794 39.0 36.0 41.0 30.5 41.0 30-31 36.677707989694234 39.0 35.5 40.0 30.0 41.0 32-33 36.37619961285222 39.0 35.0 40.0 30.0 41.0 34-35 36.068270580858 38.0 35.0 40.0 30.0 41.0 36-37 35.70653277805799 38.0 35.0 40.0 29.0 41.0 38-39 35.449534468421554 38.0 35.0 40.0 27.5 41.0 40-41 35.26587612634104 38.0 35.0 40.0 27.0 41.0 42-43 34.96728669383971 38.0 34.5 40.0 26.0 41.0 44-45 34.56668756901182 38.0 34.0 40.0 24.5 41.0 46-47 33.99882253908966 38.0 33.0 40.0 22.0 41.0 48-49 33.54211765748327 38.0 33.0 40.0 17.0 41.0 50-51 32.721146243712255 37.0 32.0 39.5 14.5 40.5 52-53 32.82744830077566 37.5 32.5 39.5 13.0 40.5 54-55 33.180703613833714 38.0 33.0 40.0 10.5 41.0 56-57 33.10503258039451 38.0 33.0 40.0 8.0 41.0 58-59 32.89068527884183 38.0 33.0 40.0 7.5 41.0 60-61 32.50728287688973 37.0 32.0 40.0 4.5 41.0 62-63 32.16531312621836 36.0 31.0 40.0 2.0 41.0 64-65 31.785178987690337 36.0 31.0 39.0 2.0 41.0 66-67 31.389785569202665 35.5 31.0 39.0 2.0 41.0 68-69 31.049148343034748 35.0 30.5 39.0 2.0 40.0 70-71 30.56984848071759 35.0 30.0 38.0 2.0 40.0 72-73 30.08930810965552 35.0 29.5 37.0 2.0 39.5 74-75 29.672383003667 35.0 29.0 37.0 2.0 39.0 76-77 29.19024087680794 34.0 29.0 36.0 2.0 39.0 78-79 28.71326185640089 34.0 28.5 36.0 2.0 38.5 80-81 28.290125686710198 34.0 27.0 35.0 2.0 37.0 82-83 27.86746833976308 34.0 26.0 35.0 2.0 37.0 84-85 27.458492372916012 34.0 26.0 35.0 2.0 36.0 86-87 27.077555652493967 34.0 25.5 35.0 2.0 36.0 88-89 26.603671087967065 33.0 24.5 35.0 2.0 35.5 90-91 26.151123955450743 33.0 23.5 35.0 2.0 35.0 92-93 25.796461142085963 33.0 22.0 35.0 2.0 35.0 94-95 25.394125645814306 33.0 19.5 35.0 2.0 35.0 96-97 24.723388701828046 32.0 12.0 35.0 2.0 35.0 98-99 24.049814605286475 32.0 2.0 35.0 2.0 35.0 100-101 22.15456602641875 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.005033371398706521 1101 2 0.03429088147792214 1101 3 0.35252272775635873 1101 4 0.38422318376365183 1101 5 0.33206878999116185 1101 6 0.12323841833381266 1101 7 0.21127422868617174 1101 8 0.21441173031711713 1101 9 0.41154221353571074 1101 10-11 0.1588196473816339 1101 12-13 0.5133696984181952 1101 14-15 0.40357109731946395 1101 16-17 0.4317222945162271 1101 18-19 0.5358878488680574 1101 20-21 0.4949002556529365 1101 22-23 0.2659951394918849 1101 24-25 0.4496828212475279 1101 26-27 0.6456983172329132 1101 28-29 0.6136952530254192 1101 30-31 0.8583770847169419 1101 32-33 0.44608335341114014 1101 34-35 0.6323069022088177 1101 36-37 0.832697873247433 1101 38-39 0.6138583350636893 1101 40-41 0.45231116177595965 1101 42-43 0.5729245000853425 1101 44-45 0.7005854062118644 1101 46-47 0.9259031121700829 1101 48-49 0.7136578928009669 1101 50-51 0.46676288032966795 1101 52-53 0.8433011517441784 1101 54-55 0.7738447455398401 1101 56-57 0.44829492665604675 1101 58-59 0.5660170622592133 1101 60-61 0.27275732229726657 1101 62-63 0.5602239823652688 1101 64-65 0.5243307953592371 1101 66-67 0.5605398337098109 1101 68-69 0.32411145458144475 1101 70-71 0.4442493234899061 1101 72-73 0.43856891875755366 1101 74-75 0.4513876254415585 1101 76-77 0.5272157165618161 1101 78-79 0.7513940324757691 1101 80-81 0.6883376435371851 1101 82-83 0.6069710457151487 1101 84-85 0.7575952095803338 1101 86-87 0.7793133335442697 1101 88-89 0.5749565005945314 1101 90-91 0.7051387102168398 1101 92-93 0.6692642347537152 1101 94-95 0.33340011091365795 1101 96-97 0.41323147461110565 1101 98-99 0.4126769610294154 1101 100-101 0.7648824828137641 1102 1 0.06425292068019317 1102 2 0.08925679167997203 1102 3 0.3898037219162056 1102 4 0.39251673825843625 1102 5 0.2938455484824942 1102 6 0.45436724843595044 1102 7 0.08097297620832933 1102 8 0.41372815653292605 1102 9 0.6383127051932647 1102 10-11 0.450807433722602 1102 12-13 0.5814026237406509 1102 14-15 0.15074657755449294 1102 16-17 0.10794032699709533 1102 18-19 0.3395492798395452 1102 20-21 0.16504923740356503 1102 22-23 -0.16186071444199968 1102 24-25 0.15607943626837795 1102 26-27 0.39574054058108743 1102 28-29 0.3345744764713672 1102 30-31 0.16270520013335243 1102 32-33 0.06938886378031128 1102 34-35 0.407700912715768 1102 36-37 0.3013348146769843 1102 38-39 0.18916923224316662 1102 40-41 -0.16764537039822613 1102 42-43 0.12117689792706443 1102 44-45 0.15857984161903005 1102 46-47 0.3887621217436674 1102 48-49 0.6754257621663697 1102 50-51 0.5584519920393092 1102 52-53 0.6265731501085767 1102 54-55 0.7570398529649296 1102 56-57 0.8340652138637097 1102 58-59 0.8670606456436616 1102 60-61 0.38237135644929054 1102 62-63 0.6272679924277647 1102 64-65 0.7932890786912346 1102 66-67 0.937729892111367 1102 68-69 0.806357647813261 1102 70-71 1.2615577850488826 1102 72-73 0.5477540525833469 1102 74-75 0.8036125736623987 1102 76-77 1.099919877561664 1102 78-79 1.1768964903190877 1102 80-81 1.1663170563215246 1102 82-83 0.8934186843188456 1102 84-85 0.795694003325945 1102 86-87 0.7470991411658048 1102 88-89 0.7721106469273202 1102 90-91 0.5681106116897681 1102 92-93 0.8838921803878215 1102 94-95 0.715014731747992 1102 96-97 0.6261504857847378 1102 98-99 0.22215410264207947 1102 100-101 0.6520861415779997 1103 1 0.13722553522829983 1103 2 0.12679219573616152 1103 3 0.17061590754239475 1103 4 0.11726026606542206 1103 5 0.025305190226603713 1103 6 -6.61311625435701E-4 1103 7 0.16304881430167484 1103 8 -0.12803678895193116 1103 9 0.3317206830651358 1103 10-11 0.04848710634757225 1103 12-13 0.20706215749645906 1103 14-15 0.08789076086934955 1103 16-17 0.16672290109695354 1103 18-19 0.16750688022602844 1103 20-21 0.22319477530840004 1103 22-23 -0.20819148068523674 1103 24-25 0.1611188898855076 1103 26-27 0.03699680437564723 1103 28-29 0.03723013591039859 1103 30-31 -0.08687563478664373 1103 32-33 -0.31527206245610984 1103 34-35 -0.13141642508865914 1103 36-37 0.1368823816249929 1103 38-39 -0.279900173915955 1103 40-41 -0.4148746181745224 1103 42-43 -0.13746690174518506 1103 44-45 0.0448132289465093 1103 46-47 -0.06795020313058586 1103 48-49 -0.12112524335618957 1103 50-51 -0.41369129137311944 1103 52-53 0.19635584735298295 1103 54-55 0.33901622815793786 1103 56-57 0.5003103472860886 1103 58-59 0.2631692780761483 1103 60-61 0.5600002556317989 1103 62-63 0.4409005670012611 1103 64-65 0.4657103845917554 1103 66-67 0.461664928628462 1103 68-69 0.5119829852766742 1103 70-71 0.7624458466590447 1103 72-73 0.3508932911711611 1103 74-75 0.43645001339261924 1103 76-77 0.2548025587025542 1103 78-79 0.3052202089053857 1103 80-81 0.36775074298497046 1103 82-83 0.03127426418755164 1103 84-85 -0.1353545250967656 1103 86-87 0.024259617451008353 1103 88-89 0.0403881389466072 1103 90-91 0.1577243167301674 1103 92-93 -0.03644750909847616 1103 94-95 -0.41721882608731775 1103 96-97 -0.30068034203076976 1103 98-99 -0.13814551750436976 1103 100-101 -0.011778986729144236 1104 1 0.23926183333616535 1104 2 0.26557968447345104 1104 3 0.11062877921185077 1104 4 -0.06786523271176748 1104 5 0.16784605777712613 1104 6 0.34280116745810574 1104 7 0.2827707862414357 1104 8 0.4698677032607108 1104 9 0.1455294100570299 1104 10-11 0.26406182638876174 1104 12-13 0.28906241492985174 1104 14-15 0.5581211637526096 1104 16-17 0.22580128956393963 1104 18-19 0.3254010751031018 1104 20-21 0.3329000140778646 1104 22-23 0.40806543783708804 1104 24-25 0.5307674933093764 1104 26-27 0.3719768532879897 1104 28-29 0.542435439038222 1104 30-31 0.3908641000569659 1104 32-33 0.3603386982595538 1104 34-35 0.3971432350992643 1104 36-37 0.7299342544528926 1104 38-39 0.8256423669515982 1104 40-41 0.772637288119725 1104 42-43 0.9118084232644676 1104 44-45 0.8403776516521333 1104 46-47 1.049915674073695 1104 48-49 0.919011196340044 1104 50-51 0.8217598606412935 1104 52-53 0.9272581513818139 1104 54-55 1.5396932779712955 1104 56-57 1.9498482543526308 1104 58-59 1.6533198766087835 1104 60-61 1.1800311476384948 1104 62-63 1.2518389117045636 1104 64-65 1.4410405429132886 1104 66-67 1.114773436801972 1104 68-69 1.2216135683633063 1104 70-71 1.2215654244682845 1104 72-73 0.9243570574216413 1104 74-75 0.7493294058498208 1104 76-77 1.3730545859904915 1104 78-79 1.4810497880017977 1104 80-81 1.322336918811974 1104 82-83 1.3157716254953158 1104 84-85 1.1982353835143833 1104 86-87 1.2993374910512152 1104 88-89 1.2491949351880827 1104 90-91 1.5927120886441841 1104 92-93 1.0888221523766184 1104 94-95 1.3846913296598835 1104 96-97 1.322017559887108 1104 98-99 1.3323629134391233 1104 100-101 1.3740647512036688 1105 1 0.19070979713707104 1105 2 0.22994943237261367 1105 3 0.04115237326485399 1105 4 0.2531560988977972 1105 5 0.4505222051591531 1105 6 0.33024137431007716 1105 7 0.47649412172947336 1105 8 0.1453298881346612 1105 9 0.3344499013304585 1105 10-11 0.37151172942689925 1105 12-13 0.16590145823948177 1105 14-15 0.5677462445541579 1105 16-17 0.35307019712696075 1105 18-19 0.3208180111082797 1105 20-21 -0.3442331468788211 1105 22-23 -0.1794264820077629 1105 24-25 -0.371559591370648 1105 26-27 -0.04777427793372624 1105 28-29 0.04536368726058271 1105 30-31 0.2491187865469371 1105 32-33 0.13399092838237436 1105 34-35 0.16406954408439844 1105 36-37 -0.119910606568439 1105 38-39 -0.506843088769152 1105 40-41 -0.9483646511175081 1105 42-43 0.007374034124197237 1105 44-45 0.3178372508764369 1105 46-47 -0.024324791343239838 1105 48-49 0.5142536009942091 1105 50-51 0.1307546893420124 1105 52-53 0.20695110548653162 1105 54-55 0.28280575373083394 1105 56-57 0.24228199708048948 1105 58-59 0.4424019709849958 1105 60-61 0.48610096017889504 1105 62-63 0.1326792478390182 1105 64-65 -0.0357985003963428 1105 66-67 -0.26669068730921097 1105 68-69 0.41275125420687075 1105 70-71 0.19961972311081766 1105 72-73 0.5639045687338609 1105 74-75 0.6213365163863429 1105 76-77 0.389796667438457 1105 78-79 0.11816355658615052 1105 80-81 0.23687150187597084 1105 82-83 0.6324296733298347 1105 84-85 0.05451851215045167 1105 86-87 0.14320303726970351 1105 88-89 0.2982511730678503 1105 90-91 0.8765937951729512 1105 92-93 0.9191485906442303 1105 94-95 0.7473157640490236 1105 96-97 0.8200815547158093 1105 98-99 0.3139789444669212 1105 100-101 0.706281945773803 1106 1 0.03528543426589792 1106 2 -0.32324205698909125 1106 3 -0.12718992273701346 1106 4 0.34305556113768176 1106 5 0.27855587361835177 1106 6 0.16616608789436782 1106 7 0.3257459337472426 1106 8 0.19852137749636256 1106 9 0.406112281503475 1106 10-11 0.3158944743415546 1106 12-13 0.23715467310972116 1106 14-15 0.12707521345759432 1106 16-17 0.11028904211176638 1106 18-19 -0.0014288909258581839 1106 20-21 0.3690881063360507 1106 22-23 0.1721634174544704 1106 24-25 0.014926263200308654 1106 26-27 0.2800548078740803 1106 28-29 -0.06809200598234355 1106 30-31 0.35585247838701406 1106 32-33 0.379373205679542 1106 34-35 -0.30451240588052997 1106 36-37 0.3319832442615862 1106 38-39 0.1560339278312668 1106 40-41 0.2622385754431633 1106 42-43 0.565475507119281 1106 44-45 0.5325321304649364 1106 46-47 0.4635756061316201 1106 48-49 0.4297317398766083 1106 50-51 0.7041414090053166 1106 52-53 0.6599847739457303 1106 54-55 1.067438674002048 1106 56-57 0.9386521163229489 1106 58-59 0.8703304255091879 1106 60-61 0.8701902751192634 1106 62-63 0.7314634423678932 1106 64-65 0.4187841509948207 1106 66-67 0.8166329039930993 1106 68-69 0.725879615207571 1106 70-71 0.5724862183177386 1106 72-73 0.8110404116143854 1106 74-75 0.2902341081562767 1106 76-77 0.526925496056645 1106 78-79 0.5572271525646428 1106 80-81 0.709077487379826 1106 82-83 0.6244217238325227 1106 84-85 0.600520382620406 1106 86-87 0.8924431588502486 1106 88-89 0.8533715845712386 1106 90-91 0.9509742020001646 1106 92-93 0.7202650273989697 1106 94-95 0.17775766257602754 1106 96-97 -0.20011367558580773 1106 98-99 0.037733912902741196 1106 100-101 0.17039230350118117 1107 1 0.19872261764989219 1107 2 0.35852086094404 1107 3 0.14440328901577004 1107 4 0.28012496336666004 1107 5 0.15510095973790783 1107 6 -0.0601890286203286 1107 7 0.4459080411433973 1107 8 0.1977108405156116 1107 9 0.34415686103741905 1107 10-11 0.18955202246718983 1107 12-13 0.4586212384592656 1107 14-15 0.48118488924280456 1107 16-17 0.5414630542698191 1107 18-19 0.5871412711815367 1107 20-21 0.9192970729014007 1107 22-23 0.6311046535233658 1107 24-25 1.0683900423289785 1107 26-27 0.9140846964252489 1107 28-29 0.3368472037441066 1107 30-31 0.7618935118216612 1107 32-33 0.7398975217889685 1107 34-35 0.08302558804044224 1107 36-37 0.40312052896269535 1107 38-39 1.1329462885202233 1107 40-41 0.4811912096883546 1107 42-43 0.6153868546370163 1107 44-45 0.0047649065541008895 1107 46-47 0.43435194858349035 1107 48-49 0.4051876669282777 1107 50-51 -0.4730685707312503 1107 52-53 -0.25989871136328446 1107 54-55 -0.12935083967575878 1107 56-57 0.17467668572517425 1107 58-59 0.3960649746479916 1107 60-61 0.11792330450123956 1107 62-63 0.02583400974378236 1107 64-65 -0.10351828061612522 1107 66-67 0.46465088778236563 1107 68-69 0.4747018036574211 1107 70-71 0.22413895388005045 1107 72-73 -0.3029223543430639 1107 74-75 -0.3335627510129271 1107 76-77 0.28947615461794385 1107 78-79 0.3231086115312074 1107 80-81 0.2927780952825607 1107 82-83 0.9100853509855114 1107 84-85 0.9872795011614421 1107 86-87 0.37713160869827433 1107 88-89 0.6202978763645497 1107 90-91 0.6524179709971278 1107 92-93 0.6612731327687733 1107 94-95 0.9261619178951754 1107 96-97 1.3489048147890692 1107 98-99 1.0264560506940263 1107 100-101 1.364775535517392 1108 1 0.49893842110274633 1108 2 0.06091129919105143 1108 3 0.10564166882984694 1108 4 0.09438459152071488 1108 5 0.33325501020271275 1108 6 0.16668680139295589 1108 7 0.38561587646876205 1108 8 0.40116369576129784 1108 9 0.7596283085805808 1108 10-11 0.31212838368897167 1108 12-13 0.37540410698516524 1108 14-15 0.8117044775562121 1108 16-17 0.8531413411224165 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2316 70-71 0.012119723110821212 2316 72-73 -0.31590312357383254 2316 74-75 -0.3223448022949782 2316 76-77 -0.5260687171769298 2316 78-79 -0.7018913884687912 2316 80-81 -1.1168372893328247 2316 82-83 -0.558504392604231 2316 84-85 -0.6992589603770192 2316 86-87 -1.066618391301727 2316 88-89 -1.1162405851739088 2316 90-91 -1.2432551059259502 2316 92-93 -1.114848662103018 2316 94-95 -1.0555688513355932 2316 96-97 -1.0288195441852928 2316 98-99 -1.2777106159726408 2316 100-101 -2.5845834388415803 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 589.0 3 214.0 4 203.0 5 223.0 6 255.0 7 349.0 8 400.0 9 560.0 10 839.0 11 1330.0 12 1959.0 13 2582.0 14 2579.0 15 2990.0 16 3938.0 17 5036.0 18 4313.0 19 2472.0 20 2429.0 21 2494.0 22 2700.0 23 3007.0 24 3271.0 25 3721.0 26 4246.0 27 5037.0 28 6003.0 29 6998.0 30 8570.0 31 10298.0 32 12811.0 33 16246.0 34 21218.0 35 28906.0 36 37023.0 37 45115.0 38 36214.0 39 6281.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.27652757231383 16.986849550403242 13.16953785952402 18.567085017758902 2 14.192915130157388 22.137321327544015 41.48063827901872 22.18912526327987 3 21.547910375525728 30.825965739838175 29.603751848998982 18.02237203563711 4 11.065693928340096 16.51825588927147 39.607609177377014 32.80844100501142 5 11.410091614869772 39.99413555727549 32.97453399842478 15.621238829429956 6 24.8061407517033 35.95961180410156 22.03993807416097 17.19430937003417 7 25.935407775065794 35.79230129402621 21.83890805459727 16.433382876310727 8 25.232150649067307 30.147880986145957 21.595123813679503 23.024844551107233 9 27.771453112644178 18.86980885119843 22.993073923595013 30.365664112562374 10-11 23.68602300120359 26.362052446562945 27.777720951007367 22.174203601226093 12-13 29.208688773875036 24.577221801717027 27.2698756895717 18.94421373483624 14-15 24.75278742616005 25.06996260707432 30.57050628726084 19.60674367950479 16-17 19.28960398149016 28.824045746231135 31.226404642799316 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18.911071275210787 28.847477728626636 31.72043377205837 20.521017224104206 44-45 19.405195212388897 28.600762037188503 30.86523256448007 21.128810185942527 46-47 20.19538478840983 28.07689292859066 29.8051264224442 21.92259586055531 48-49 20.893037585349603 27.980015370170623 30.03914111077599 21.087805933703784 50-51 20.883879048130254 28.824987858804285 30.87868176435004 19.41245132871542 52-53 19.89261328684794 29.4705129581444 30.457475577276007 20.179398177731656 54-55 18.693276895655046 28.47751183453127 31.323422658909895 21.505788610903785 56-57 20.57381127651453 29.491623043786465 28.965933256993292 20.96863242270571 58-59 20.821575603904247 28.324540596542885 29.383588254539504 21.47029554501336 60-61 20.477359747671052 27.653603202164767 31.3037506837317 20.56528636643248 62-63 21.967586320888948 27.75133722188426 30.072710108698804 20.20836634852799 64-65 20.784171386895146 29.05701081733227 30.617753512054474 19.54106428371811 66-67 19.840231201265063 28.730437864660015 30.35232291837068 21.077008015704237 68-69 19.11800541554695 30.454186824441194 29.88587816893848 20.54192959107338 70-71 20.04434113705535 29.95310268671163 28.33845136619099 21.664104810042023 72-73 19.85028108507872 28.90975797202952 28.895784661053487 22.344176281838273 74-75 19.65012073322643 30.221988954230433 29.13615674700212 20.991733565541015 76-77 20.76654886016243 28.18716579362618 29.52774019405696 21.518545152154427 78-79 19.792964615119754 28.424325379306346 30.74320350865829 21.039506496915607 80-81 20.005453817245993 30.186878456569723 29.441239379969154 20.366428346215137 82-83 19.698482700329905 29.694018049458787 29.062321073152113 21.545178177059192 84-85 19.867009084758703 29.126782741173844 28.77436704061131 22.231841133456147 86-87 20.581403869323015 29.181811830183307 29.205498780718482 21.031285519775196 88-89 21.024935798795894 27.894933259717085 29.166176743742195 21.91395419774482 90-91 19.652208885987978 28.203153092508547 30.433085828125613 21.71155219337786 92-93 19.80030130337569 30.035583792332442 29.38781561869444 20.776299285597428 94-95 19.173558225650062 30.280200876234815 28.736650710854438 21.80959018726068 96-97 18.54103757830233 29.36175506281227 28.654733584627863 23.442473774257532 98-99 19.774475432104868 29.89581078570564 27.818332733957025 22.511381048232465 100-101 20.46933358789947 29.3572148191586 28.575276286652834 21.598175306289093 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 499.0 1 376.0 2 799.5 3 1096.5 4 675.5 5 559.5 6 760.5 7 1136.5 8 1468.5 9 1511.0 10 1250.0 11 1037.5 12 1053.5 13 1073.5 14 1102.0 15 1119.0 16 1086.0 17 1077.0 18 1130.0 19 1175.0 20 1189.0 21 1291.5 22 1470.0 23 1656.0 24 1797.0 25 2067.0 26 2508.0 27 2835.5 28 3133.5 29 3581.5 30 4059.0 31 4526.0 32 5081.0 33 5575.5 34 6122.0 35 6460.5 36 6786.0 37 7306.5 38 7496.0 39 7350.5 40 7238.5 41 7292.5 42 7362.0 43 7543.5 44 8160.5 45 8826.0 46 9507.5 47 12923.0 48 24178.5 49 23893.5 50 13337.5 51 9756.5 52 7277.5 53 6143.0 54 5453.0 55 4846.0 56 4397.0 57 3895.5 58 3500.5 59 3143.5 60 2763.5 61 2437.5 62 2036.0 63 1464.5 64 951.0 65 715.0 66 531.5 67 394.5 68 294.5 69 222.0 70 187.5 71 140.5 72 120.5 73 122.0 74 93.5 75 78.5 76 57.0 77 26.5 78 25.5 79 23.5 80 15.0 81 8.0 82 5.0 83 2.5 84 1.0 85 1.0 86 0.5 87 0.0 88 0.5 89 1.5 90 1.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018403151710129913 2 0.004771187480404051 3 0.00886077674932181 4 0.03339831236282836 5 0.04600787927532478 6 0.0592990443993075 7 0.02726392845945172 8 0.02862712488242431 9 0.014995160652698448 10-11 0.04737107569829736 12-13 0.04430388374660905 14-15 0.01942554902735935 16-17 0.02402633695489183 18-19 0.027604727565194866 20-21 0.019595948580230928 22-23 0.012779966465367994 24-25 0.0022151941873304526 26-27 0.0015335959758441594 28-29 0.0011927968701010128 30-31 0.0056231852447619175 32-33 0.001874395081587306 34-35 0.0011927968701010128 36-37 0.004771187480404051 38-39 0.005452785691890344 40-41 0.003748790163174612 42-43 6.81598211486293E-4 44-45 0.001363196422972586 46-47 0.0023855937402020256 48-49 8.519977643578662E-4 50-51 0.001874395081587306 52-53 8.519977643578662E-4 54-55 0.0023855937402020256 56-57 0.001363196422972586 58-59 0.001363196422972586 60-61 5.111986586147198E-4 62-63 8.519977643578662E-4 64-65 0.003407991057431465 66-67 0.001363196422972586 68-69 0.004600787927532478 70-71 0.007156781220606078 72-73 0.003919189716046185 74-75 0.004259988821789331 76-77 0.003067191951688319 78-79 0.032887113704213634 80-81 0.018914350368744632 82-83 0.004089589268917758 84-85 0.00834957809070709 86-87 0.0059639843505050645 88-89 0.004941587033275625 90-91 0.011416770042395408 92-93 0.012268767806753275 94-95 0.004600787927532478 96-97 0.004771187480404051 98-99 0.02300393963766239 100-101 0.040384694030562863 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 293428.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.14211648987606 #Duplication Level Percentage of deduplicated Percentage of total 1 90.17412914042315 58.74133624838572 2 6.289016855660437 8.193597372364524 3 1.4029445570904795 2.7417233330047672 4 0.6178703762393355 1.6099753609850536 5 0.37390756047857526 1.2178564930570364 6 0.23244298796189578 0.9085096919441202 7 0.1698048559341041 0.7743013394064221 8 0.10501084992742829 0.5472503214938741 9 0.0827757721053343 0.4852970090122607 >10 0.46741875415123835 5.541822498608258 >50 0.03606667908602222 1.6722024824630997 >100 0.03449856260402126 5.008775914687884 >500 0.006795171422004188 2.996412511845913 >1k 0.00731787691600451 9.560939422741066 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3660 1.2473247270199164 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2932 0.9992229780389056 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2852 0.9719590495794539 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2570 0.8758537017598865 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2496 0.8506345679348937 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2331 0.7944027154872746 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2054 0.7000013631964229 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1770 0.6032144171653694 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1512 0.5152882478836375 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1347 0.45905639543601834 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1194 0.406914132257317 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1173 0.3997573510367109 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1115 0.3799910029036084 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1073 0.3656774404623962 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 980 0.3339831236282836 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 850 0.28967923988167454 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 825 0.2811592622380959 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 709 0.24162656597189086 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 708 0.24128576686614772 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 700 0.23855937402020255 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 665 0.22663140531919246 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 627 0.2136810393009529 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 583 0.19868587864825443 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 565 0.1925514947448778 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 562 0.19152909742764834 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 514 0.1751707403519773 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 512 0.17448914214049102 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 499 0.17005875376583013 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 490 0.1669915618141418 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 487 0.16596916449691235 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 424 0.14449882083509413 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 402 0.1370012405087449 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 401 0.13666044140300176 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 394 0.13427484766279973 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 377 0.12848126286516623 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 374 0.12745886554793678 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 374 0.12745886554793678 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 342 0.11655329416415611 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 321 0.10939651294355004 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 308 0.10496612456888912 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.7039955287157326E-4 0.0 18-19 0.0 0.0 0.0 3.407991057431465E-4 0.0 20-21 0.0 0.0 0.0 3.407991057431465E-4 0.0 22-23 0.0 0.0 0.0 6.81598211486293E-4 0.0 24-25 0.0 0.0 0.0 8.519977643578662E-4 0.0 26-27 0.0 0.0 0.0 0.0017039955287157325 0.0 28-29 0.0 0.0 0.0 0.002726392845945172 0.0 30-31 0.0 0.0 0.0 0.0059639843505050645 0.0 32-33 0.0 0.0 0.0 0.00937197540793653 0.0 34-35 0.0 0.0 0.0 0.014313562441212154 0.0 36-37 0.0 0.0 0.0 0.021129544556075082 0.0 38-39 0.0 0.0 0.0 0.02964952219965375 0.0 40-41 0.0 0.0 0.0 0.03459110923292937 0.0 42-43 0.0 0.0 0.0 0.050267868097114116 0.0 44-45 0.0 0.0 0.0 0.06781902204288616 0.0 46-47 0.0 0.0 0.0 0.08196218493122674 0.0 48-49 0.0 0.0 0.0 0.09951333887699879 0.0 50-51 0.0 0.0 0.0 0.12353967583189063 0.0 52-53 0.0 0.0 0.0 0.16988835421295856 0.0 54-55 0.0 0.0 0.0 0.2009010728355849 0.0 56-57 0.0 0.0 0.0 0.2591777199176629 0.0 58-59 0.0 1.7039955287157326E-4 0.0 0.34522949411780746 0.0 60-61 0.0 3.407991057431465E-4 0.0 0.44917322136946713 0.0 62-63 0.0 3.407991057431465E-4 0.0 0.5687937074853116 0.0 64-65 0.0 3.407991057431465E-4 0.0 0.6645582561991358 0.0 66-67 0.0 3.407991057431465E-4 0.0 0.8049674877653121 0.0 68-69 0.0 3.407991057431465E-4 0.0 0.9724702482380686 0.0 70-71 0.0 3.407991057431465E-4 0.0 1.1086194909824556 0.0 72-73 0.0 3.407991057431465E-4 0.0 1.3108837602410133 0.0 74-75 0.0 3.407991057431465E-4 0.0 1.508547241572038 0.0 76-77 0.0 3.407991057431465E-4 0.0 1.647082078056627 0.0 78-79 0.0 3.407991057431465E-4 0.0 1.792092097550336 0.0 80-81 0.0 3.407991057431465E-4 0.0 2.0504178197036413 0.0 82-83 0.0 3.407991057431465E-4 0.0 2.2627356625816217 0.0 84-85 0.0 3.407991057431465E-4 0.0 2.611884346415475 0.0 86-87 0.0 3.407991057431465E-4 0.0 3.0656583557124746 0.0 88-89 0.0 3.407991057431465E-4 0.0 3.5616914541216245 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 35 3.3988035E-6 67.89699 2 TACCTGG 675 0.0 61.962284 2 ACCTGGG 705 0.0 55.280663 3 GTACCTG 770 0.0 54.31758 1 ATAACGC 50 2.812031E-5 47.52789 3 GAGTACG 80 2.244542E-7 41.5869 1 CCTGGGA 615 0.0 40.18618 4 AGTACTC 110 1.6025297E-9 38.886456 3 CCTGGGG 275 0.0 36.29402 4 GCTTATG 90 2.397245E-5 31.685255 1 AGTACGC 90 2.397245E-5 31.685255 2 ACGCACG 30 0.0039495802 31.666353 72-73 TAACGCA 80 4.4701772E-4 29.70493 4 GTACGCA 80 4.4701772E-4 29.70493 3 CGCACGG 40 4.489007E-4 29.689737 74-75 TACGCAG 65 0.0058203507 29.24793 4 TCATAGG 65 0.0058203507 29.24793 2 CTAAGGG 70 0.008357594 27.158794 3 TATACCA 125 7.3328556E-6 26.615616 5 GTACCAG 130 9.942518E-6 25.591938 1 >>END_MODULE