##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936733_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 314229 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7012147192016 31.0 31.0 34.0 30.0 34.0 2 31.753246835906296 31.0 31.0 34.0 30.0 34.0 3 31.851258158858666 31.0 31.0 34.0 30.0 34.0 4 35.63098568241633 37.0 35.0 37.0 33.0 37.0 5 35.429861661399805 37.0 35.0 37.0 33.0 37.0 6 35.584007204936526 37.0 35.0 37.0 33.0 37.0 7 35.54896269917799 37.0 35.0 37.0 33.0 37.0 8 35.554242288267474 37.0 35.0 37.0 33.0 37.0 9 37.126461911535856 39.0 37.0 39.0 33.0 39.0 10-11 36.955723373717895 39.0 37.0 39.0 33.0 39.0 12-13 36.84382090768197 39.0 37.0 39.0 32.0 39.0 14-15 37.699031597974724 39.0 37.0 41.0 32.0 41.0 16-17 37.69288798933262 39.0 36.5 41.0 32.5 41.0 18-19 38.001236359470326 40.0 37.0 41.0 33.0 41.0 20-21 38.04512791626489 40.0 37.0 41.0 33.0 41.0 22-23 37.91108713708792 40.0 37.0 41.0 33.0 41.0 24-25 37.733116612406874 39.5 36.5 41.0 32.5 41.0 26-27 37.36286752654911 39.0 36.0 41.0 32.0 41.0 28-29 37.20680618275207 39.0 36.0 41.0 32.0 41.0 30-31 37.046046036489315 39.0 35.5 41.0 31.5 41.0 32-33 36.73014425785017 39.0 35.0 41.0 31.0 41.0 34-35 36.40843938656203 39.0 35.0 40.0 30.0 41.0 36-37 35.99184034573511 38.0 35.0 40.0 30.0 41.0 38-39 35.73525995372802 38.0 35.0 40.0 30.0 41.0 40-41 35.467692351756206 38.0 35.0 40.0 28.5 41.0 42-43 35.169419436143706 38.0 35.0 40.0 27.0 41.0 44-45 34.75975960207364 38.0 34.0 40.0 25.0 41.0 46-47 34.151940145562634 38.0 33.5 40.0 22.5 41.0 48-49 33.58521492287472 38.0 33.0 40.0 17.5 41.0 50-51 33.18070738219579 38.0 33.0 40.0 13.0 41.0 52-53 32.80576744985345 37.0 32.5 40.0 8.5 41.0 54-55 32.58725006285225 37.0 32.0 40.0 7.5 41.0 56-57 32.297836927845616 37.0 31.5 40.0 4.5 41.0 58-59 31.992900082423965 36.5 31.0 39.5 2.0 41.0 60-61 31.54512791626489 36.0 31.0 39.0 2.0 40.0 62-63 31.104210623462507 35.5 30.0 39.0 2.0 40.0 64-65 30.605997536828237 35.0 29.0 38.5 2.0 40.0 66-67 30.133954854580576 35.0 29.0 38.0 2.0 40.0 68-69 29.896685220014703 35.0 29.0 37.5 2.0 39.5 70-71 29.409548450333993 34.0 28.5 37.0 2.0 39.0 72-73 28.90242625601074 34.0 27.5 36.0 2.0 39.0 74-75 28.361764827562062 34.0 26.0 36.0 2.0 38.5 76-77 26.957293884396414 31.5 25.5 34.5 2.0 36.5 78-79 27.68631316651232 33.0 26.0 35.0 2.0 37.0 80-81 27.47427990414634 33.0 26.0 35.0 2.0 37.0 82-83 27.11151580535215 33.0 25.5 35.0 2.0 36.0 84-85 26.681946924058572 33.0 25.0 35.0 2.0 36.0 86-87 26.22778451384182 32.0 24.0 35.0 2.0 35.0 88-89 25.696759369758997 32.0 21.5 34.5 2.0 35.0 90-91 25.08817932145028 32.0 19.5 34.0 2.0 35.0 92-93 24.612686925777062 31.0 18.0 34.0 2.0 35.0 94-95 24.03913228887213 31.0 6.0 34.0 2.0 35.0 96-97 23.134406754309754 31.0 2.0 34.0 2.0 35.0 98-99 22.16334106654701 30.5 2.0 34.0 2.0 35.0 100-101 19.193067794506554 26.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26087074290702006 1101 2 0.28676177422928006 1101 3 0.29969567128148 1101 4 0.18665762470262592 1101 5 0.2484125167307809 1101 6 0.21641257032221972 1101 7 0.18548257457177186 1101 8 0.1960457682128549 1101 9 0.3169027355842928 1101 10-11 0.23161279515329625 1101 12-13 0.260485290052749 1101 14-15 0.4020780083286155 1101 16-17 0.4262775211974983 1101 18-19 0.29111294380919617 1101 20-21 0.15738676886930847 1101 22-23 0.10451448241091299 1101 24-25 0.15202374984597355 1101 26-27 0.19444709139403926 1101 28-29 0.44306977780228607 1101 30-31 0.2792434721263959 1101 32-33 0.16323926232880837 1101 34-35 -0.17912786492846777 1101 36-37 -0.16234674906482383 1101 38-39 0.178093784041927 1101 40-41 0.3085904617463555 1101 42-43 0.6721480349746756 1101 44-45 0.7204003478628707 1101 46-47 0.5362098599859522 1101 48-49 0.6218841237153896 1101 50-51 0.6389381142813377 1101 52-53 0.9061661763177611 1101 54-55 0.6029481668584324 1101 56-57 0.6054649871666982 1101 58-59 0.4868552103846788 1101 60-61 0.4305565269921168 1101 62-63 0.3810020309205626 1101 64-65 0.33076321135216347 1101 66-67 0.3682783881669991 1101 68-69 0.46707405834195015 1101 70-71 0.2721392218762553 1101 72-73 0.21283936760340794 1101 74-75 0.057485007016548906 1101 76-77 0.07791523497203912 1101 78-79 0.2973123583606636 1101 80-81 0.29885719511198516 1101 82-83 0.4195303531936041 1101 84-85 0.43179682501718375 1101 86-87 0.6588555168039569 1101 88-89 0.32208261766831825 1101 90-91 0.4026859651024459 1101 92-93 0.20048861612070112 1101 94-95 0.3667701694252514 1101 96-97 0.4779628408869705 1101 98-99 0.8134610694692341 1101 100-101 0.8469676203264811 1102 1 0.22500948482354133 1102 2 0.31545333673726716 1102 3 0.23597356408111025 1102 4 0.2723105654280289 1102 5 0.09084535674816152 1102 6 0.005679753309209445 1102 7 0.23316552322090445 1102 8 0.1879814582161785 1102 9 0.4765530691476698 1102 10-11 0.3277455048125759 1102 12-13 0.07852071678382799 1102 14-15 0.31531957978274505 1102 16-17 0.38609016337728974 1102 18-19 0.12888739873341137 1102 20-21 0.24636895326211317 1102 22-23 0.36089180521334896 1102 24-25 0.22230210273303186 1102 26-27 0.021435052439976232 1102 28-29 0.009686601665485739 1102 30-31 0.11391233698849845 1102 32-33 0.15863838816271425 1102 34-35 0.17970836951162994 1102 36-37 0.3656674625242644 1102 38-39 0.25825762628306137 1102 40-41 0.02997558047447768 1102 42-43 0.16597519546850492 1102 44-45 0.12092561433016868 1102 46-47 0.46683251162310313 1102 48-49 0.036180062165918514 1102 50-51 0.21529345512943365 1102 52-53 0.2849011915262025 1102 54-55 0.34680727786729904 1102 56-57 0.1943704903234078 1102 58-59 0.12218481189785635 1102 60-61 0.5450160520796601 1102 62-63 0.35784429761790193 1102 64-65 0.9177708539153571 1102 66-67 0.47043696496325893 1102 68-69 0.3873255727963638 1102 70-71 0.30704835461147795 1102 72-73 0.46480112922700556 1102 74-75 0.07648278326070113 1102 76-77 -0.2803842308153506 1102 78-79 -0.2001379905389591 1102 80-81 0.20402167636342128 1102 82-83 -0.03005812417265119 1102 84-85 0.24780019899157324 1102 86-87 0.31062685335790263 1102 88-89 0.18586479183696625 1102 90-91 0.39461526500275923 1102 92-93 0.4048057697132208 1102 94-95 0.8365449821952353 1102 96-97 0.8849932322168748 1102 98-99 0.9026221767462843 1102 100-101 1.0219861516252031 1103 1 -0.024594569658560772 1103 2 -0.058374320229305 1103 3 -0.06484403472164146 1103 4 -0.17774521124493248 1103 5 -0.22584678815183423 1103 6 0.11852766891428246 1103 7 -0.09679366569904602 1103 8 -0.15748502298730926 1103 9 -0.0734232888880797 1103 10-11 -0.22173276523833607 1103 12-13 -0.17677347647431674 1103 14-15 -0.1985511376934852 1103 16-17 0.08823813912017187 1103 18-19 -0.21437326425153458 1103 20-21 -0.19720020292633933 1103 22-23 -0.20918940378619766 1103 24-25 -0.25924339312847877 1103 26-27 0.17392342656143 1103 28-29 0.11835241446703293 1103 30-31 0.11854662105014313 1103 32-33 0.24766071260391698 1103 34-35 0.04528199701526603 1103 36-37 0.29484485988830045 1103 38-39 0.1678718720585053 1103 40-41 -0.17365977321389892 1103 42-43 -0.043928721752493516 1103 44-45 -0.06645875810192337 1103 46-47 0.10138053694524984 1103 48-49 -0.0206341028136805 1103 50-51 0.35829290883938114 1103 52-53 0.2801613219931127 1103 54-55 0.006450215346923471 1103 56-57 -0.20019093976294045 1103 58-59 0.02916630689631816 1103 60-61 0.045071140151716094 1103 62-63 0.17369864475538677 1103 64-65 -0.0470163913596906 1103 66-67 0.36346740571373815 1103 68-69 0.5238751314031873 1103 70-71 0.6798545869086929 1103 72-73 1.0331746079928479 1103 74-75 0.4718659437552084 1103 76-77 0.3517123753003446 1103 78-79 0.6843055969799217 1103 80-81 0.690805129742845 1103 82-83 0.9680941300771622 1103 84-85 1.0552536151406393 1103 86-87 0.31753581572816003 1103 88-89 0.15300475685604198 1103 90-91 0.2974123618613618 1103 92-93 0.5023965846953402 1103 94-95 0.48579693208345276 1103 96-97 0.3621821797133542 1103 98-99 0.5431495532227579 1103 100-101 1.2576758772407004 1104 1 0.12487597347507773 1104 2 0.3410962229043335 1104 3 0.07141598973076668 1104 4 0.1278425791274529 1104 5 0.25223929262401157 1104 6 0.14829019041375346 1104 7 0.1907708327156854 1104 8 0.010754817864793154 1104 9 0.032980963344762415 1104 10-11 0.13547706960428485 1104 12-13 0.10896227170729844 1104 14-15 0.24518500421338985 1104 16-17 0.21887203679305145 1104 18-19 -0.013860903350526144 1104 20-21 0.03997782305952313 1104 22-23 0.06880912492466251 1104 24-25 -0.08568826123052276 1104 26-27 0.28848193620770246 1104 28-29 0.08419415763382432 1104 30-31 -0.14839392643519744 1104 32-33 0.007951965686373796 1104 34-35 -0.1343940085383366 1104 36-37 -0.18743232269660837 1104 38-39 0.09025100876405645 1104 40-41 0.19479195399666338 1104 42-43 0.05187862225977824 1104 44-45 -0.32296817642465214 1104 46-47 0.024109363851188448 1104 48-49 0.5121383447068908 1104 50-51 -0.05302565634286793 1104 52-53 0.2721184473842513 1104 54-55 0.13991789879181482 1104 56-57 -0.26478519551858426 1104 58-59 -0.4694349323021392 1104 60-61 -0.40435847627698607 1104 62-63 -0.09387393538842659 1104 64-65 0.1103053443470472 1104 66-67 -0.011884874784424682 1104 68-69 0.042561539592668396 1104 70-71 -0.24231214537885037 1104 72-73 0.23071848763725455 1104 74-75 0.3532044024244172 1104 76-77 0.3470731519752306 1104 78-79 0.26259734739954865 1104 80-81 0.346191913007452 1104 82-83 0.15296368268093374 1104 84-85 -0.026522085793939 1104 86-87 -0.041127176169389656 1104 88-89 0.2909251621858395 1104 90-91 0.7476294288153795 1104 92-93 0.5826854547038351 1104 94-95 0.6956421358880824 1104 96-97 0.7844654754010243 1104 98-99 1.2093016140636728 1104 100-101 1.602395296185101 1105 1 0.21519487195549303 1105 2 0.2959189381786409 1105 3 0.3172246506425189 1105 4 -0.04875845295264014 1105 5 0.08237412889425855 1105 6 0.2792561603074546 1105 7 0.12684845274850431 1105 8 0.05419611533690727 1105 9 0.21625246699508693 1105 10-11 0.1562821634189291 1105 12-13 0.14838463978416172 1105 14-15 0.44270408818285745 1105 16-17 0.36081872058178277 1105 18-19 0.2071356246749687 1105 20-21 0.37832887297511064 1105 22-23 0.31377060027602255 1105 24-25 0.1031122429192024 1105 26-27 0.14353294569617958 1105 28-29 0.20885528340966886 1105 30-31 -0.07318168415796578 1105 32-33 0.035023260067006845 1105 34-35 0.11439615102981548 1105 36-37 -0.005968148241777271 1105 38-39 -0.013272094134805457 1105 40-41 -0.19955360258939692 1105 42-43 -0.2907678070760298 1105 44-45 -0.06442200629790307 1105 46-47 0.25430900238534804 1105 48-49 0.38715915277328605 1105 50-51 0.1548885430816398 1105 52-53 0.37639361325427245 1105 54-55 0.5464659000284087 1105 56-57 0.33366371176067133 1105 58-59 0.08428554996939042 1105 60-61 0.4160589929865246 1105 62-63 0.6755548534632076 1105 64-65 0.5655353032065271 1105 66-67 0.8304167351653966 1105 68-69 0.4503461344893438 1105 70-71 0.4698350092666992 1105 72-73 0.7844003263569022 1105 74-75 0.7151280189852578 1105 76-77 0.44987986287390314 1105 78-79 0.6768948670780333 1105 80-81 0.5940932708824107 1105 82-83 0.8350818249367649 1105 84-85 0.7435487805350398 1105 86-87 0.9259604079546477 1105 88-89 0.8765622457272535 1105 90-91 0.6511780959100975 1105 92-93 1.122220780855411 1105 94-95 1.1032543210917645 1105 96-97 1.5516678011483336 1105 98-99 1.7002530777625147 1105 100-101 1.562113852224197 1106 1 -0.04952467666714355 1106 2 0.07611586261718628 1106 3 -0.017856663662161765 1106 4 0.10378054472409559 1106 5 -0.13399936374873533 1106 6 -0.09618360072806098 1106 7 -0.143646070981994 1106 8 -0.13458582956850051 1106 9 -0.012887924641155735 1106 10-11 -0.12691288028059233 1106 12-13 0.1683757892475981 1106 14-15 0.1354023955177368 1106 16-17 0.25151459401911325 1106 18-19 0.3166720778224388 1106 20-21 0.10806667582940577 1106 22-23 0.2668959460001048 1106 24-25 -0.10192149975144815 1106 26-27 0.04068971082506323 1106 28-29 0.1119640343776993 1106 30-31 0.17871564961789943 1106 32-33 -0.07428086649566978 1106 34-35 0.23664418731701176 1106 36-37 0.012872431468366585 1106 38-39 0.4296861977116393 1106 40-41 -0.059051334639399045 1106 42-43 -0.2775030654010635 1106 44-45 -0.18880523453110953 1106 46-47 0.1653832362607801 1106 48-49 0.3115423810064968 1106 50-51 -0.48325726950825043 1106 52-53 -0.4614756200680077 1106 54-55 -0.3337310244101346 1106 56-57 -0.6054224703391178 1106 58-59 -0.1649787491787471 1106 60-61 -0.0843835338416632 1106 62-63 -0.32248696532205656 1106 64-65 -0.6441339079894028 1106 66-67 -0.39664378037835846 1106 68-69 -0.12530382678955831 1106 70-71 -0.15257897458107195 1106 72-73 -0.09793179002765129 1106 74-75 0.21913288678036835 1106 76-77 -0.6242765703598607 1106 78-79 -0.5407177006838886 1106 80-81 -0.4876967501376157 1106 82-83 0.1699418758273481 1106 84-85 -0.04619566022168087 1106 86-87 -0.2328514075116601 1106 88-89 -0.00916626406365495 1106 90-91 -0.1950327681235322 1106 92-93 -0.5496455760424759 1106 94-95 -0.6684239619041428 1106 96-97 -0.7829156704746332 1106 98-99 -0.4530714050342546 1106 100-101 -0.41072606369570863 1107 1 -0.009598161051709297 1107 2 -0.0067581933125957505 1107 3 -0.27578685349682175 1107 4 -0.4485362546227094 1107 5 -0.5156690922647584 1107 6 -0.33353816288359894 1107 7 -0.2633031438540101 1107 8 -0.4858820124629659 1107 9 -0.17741129675791 1107 10-11 -0.29411026750246805 1107 12-13 -0.4314813244490736 1107 14-15 -0.39178682677252397 1107 16-17 -0.544402940171544 1107 18-19 -0.21827382950558416 1107 20-21 -0.40630360381950226 1107 22-23 -0.35861946530832967 1107 24-25 -0.14166430440545952 1107 26-27 -0.2938071252639247 1107 28-29 -0.5874031834218485 1107 30-31 -0.3287348504841603 1107 32-33 -0.4016537997473506 1107 34-35 -0.1949540980473472 1107 36-37 -0.3928226083356776 1107 38-39 -0.5348575867341694 1107 40-41 -0.11328689291659799 1107 42-43 -0.502099921902392 1107 44-45 -0.4200360979726341 1107 46-47 -0.2832389315285653 1107 48-49 -0.8652079762092626 1107 50-51 -0.30309805334168516 1107 52-53 -0.60115718773487 1107 54-55 -0.7194015694193183 1107 56-57 -0.7543304107351183 1107 58-59 -0.42092894309587336 1107 60-61 -0.5631547116330502 1107 62-63 -0.7582085411538593 1107 64-65 -0.7046972882710918 1107 66-67 -0.6421632741525976 1107 68-69 -0.5441239941706542 1107 70-71 -1.03790250999646 1107 72-73 -1.080703784312199 1107 74-75 -1.0889503955221436 1107 76-77 -1.651363731004892 1107 78-79 -1.3775007176261198 1107 80-81 -0.8476477605479005 1107 82-83 -0.9028280772242923 1107 84-85 -1.2912252580987982 1107 86-87 -0.7106019688507104 1107 88-89 -0.9515422591646931 1107 90-91 -1.3162411780031036 1107 92-93 -0.755605976124123 1107 94-95 -0.5740169400629505 1107 96-97 0.1268210104806613 1107 98-99 0.2970204504464533 1107 100-101 0.6901108417952102 1108 1 0.0675253307523569 1108 2 -0.01062302050098296 1108 3 0.21879565864085038 1108 4 -0.3167764122922776 1108 5 0.036520495775427264 1108 6 -0.0751715811405802 1108 7 -0.1808919531603621 1108 8 -0.058501346212906924 1108 9 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0.7085555019330947 2316 86-87 0.35653196683890087 2316 88-89 0.6808255324908998 2316 90-91 0.9709366056801301 2316 92-93 0.6128168248325565 2316 94-95 0.49010078515628663 2316 96-97 0.6150949940044477 2316 98-99 0.31151021924794264 2316 100-101 0.19021049632616993 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 8.0 5 26.0 6 60.0 7 126.0 8 258.0 9 511.0 10 873.0 11 1430.0 12 2188.0 13 2873.0 14 2996.0 15 3734.0 16 4849.0 17 6252.0 18 5334.0 19 3043.0 20 2660.0 21 2782.0 22 2893.0 23 3186.0 24 3601.0 25 4284.0 26 5066.0 27 6111.0 28 7270.0 29 8797.0 30 10639.0 31 12796.0 32 15844.0 33 19555.0 34 24916.0 35 32233.0 36 41514.0 37 47857.0 38 26231.0 39 1432.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.567470941985846 17.869365483122316 13.916011117018398 17.647152457873442 2 14.662300215776508 23.762467617610927 40.41831363338362 21.156918533228946 3 21.7903959315259 31.36136668141213 29.798453954382424 17.049783432679547 4 11.604912340999716 17.323671589827804 38.56582301442579 32.5055930547467 5 11.12956916359563 41.04529860673528 32.74458510753407 15.080547122135023 6 24.841834591443156 37.00975088152552 20.820550682943594 17.32786384408773 7 25.89321800343062 35.74940568820828 21.907589687775474 16.449786620585623 8 25.576888193005736 30.6006129287876 21.262200497089704 22.560298381116954 9 27.363687572201904 19.06798674826477 22.899342186918208 30.668983492615116 10-11 24.03414081494965 26.750957903178584 27.64171238718383 21.573188894687934 12-13 29.679257982748002 24.742341860676046 27.486558177354087 18.09184197922186 14-15 24.339483935027243 25.544675136208568 31.25493278680177 18.860908141962422 16-17 18.80448100822685 28.61623410721958 31.976831150645257 20.60245373390831 18-19 18.275047815446698 29.066063285056437 34.58401356972144 18.07487532977542 20-21 20.962100888205732 25.798064468906436 33.21065210403877 20.029182538849057 22-23 19.555282570921197 26.090789314561043 32.99132460489717 21.362603509620588 24-25 21.026686334133714 26.146115277452903 33.91858368777737 18.908614700636008 26-27 21.64130178795343 24.033461271617433 36.006034104145584 18.31920283628355 28-29 18.85499596605439 26.42162767716341 36.00077655442166 18.722599802360534 30-31 19.239747378068863 27.413521224474856 34.7702041056633 18.576527291792967 32-33 19.43550722562208 26.819930687492246 34.192738416887046 19.55182366999863 34-35 20.57637988240068 25.4175253220455 34.240179502072706 19.765915293481115 36-37 21.6086023832561 25.07574985995824 35.31741737536284 17.998230381422825 38-39 21.700129373928846 25.126549344298553 35.188753856950306 17.98456742482229 40-41 19.442053188256132 26.662232455561092 35.51803095567327 18.377683400509508 42-43 18.916360095217485 27.938312308260244 33.089190014893646 20.056137581628626 44-45 19.828783744003058 27.723189777943976 31.824582899060378 20.623443578992593 46-47 20.7531768232722 27.273898971768993 30.882732020278205 21.090192184680596 48-49 21.311344983085476 27.473450721930327 31.023591225451664 20.191613069532536 50-51 21.451382689764802 28.337542107633233 31.541143354512457 18.6699318480895 52-53 20.194260036025945 28.98942772215468 31.242958710194834 19.57335353162454 54-55 18.8632468704202 27.996989365714093 31.881057668286022 21.258706095579683 56-57 21.215245787645458 28.781651335271434 29.6657326211037 20.337370255979405 58-59 21.403009631648708 27.65172671131627 30.127981745535436 20.81728191149958 60-61 20.83944637338926 27.152877451713614 31.993450427886284 20.01422574701084 62-63 22.399353289487294 27.091028005556872 30.866741670658215 19.64287703429762 64-65 20.944777133617983 28.604938723481453 31.233598724340805 19.21668541855976 66-67 20.238943911194845 27.882571224889887 30.971051760572344 20.907433103342925 68-69 19.692502072635325 29.777523881055394 30.347359359161846 20.18261468714743 70-71 20.87569893294381 29.081020529613756 28.953088352761842 21.090192184680596 72-73 20.487042641690774 28.095810936818754 29.659827574684222 21.757318846806246 74-75 20.370903755663093 29.71590249056748 29.47800086249286 20.435192891276568 76-77 21.22460540616233 27.524421933354652 30.25312245894935 20.99785020153366 78-79 20.299225203405985 27.9418480864004 31.094979018909235 20.663947691284374 80-81 20.345345584280427 29.763022466081278 29.918493222620757 19.97313872701754 82-83 20.122848754405926 28.915605133550283 29.715395081275908 21.246151030767884 84-85 20.05903835902006 28.634718056030746 29.373572775518973 21.932670809430224 86-87 20.70652822532522 28.546604606754983 29.67848191908342 21.06838524883638 88-89 21.357254935660567 27.33240396826155 29.490154140926116 21.82018695515177 90-91 20.240724943980446 27.58071908739051 30.818713078019965 21.359842890609084 92-93 19.96502472774026 29.487432452215312 29.74743970823176 20.800103111812668 94-95 19.488827042328825 29.742201684496617 28.915865870405465 21.853105402769092 96-97 19.00348159274912 28.73507434187077 29.01162864707979 23.249815418300322 98-99 20.134301876884642 29.08176661070755 28.144358605106333 22.63957290730147 100-101 20.441684754587754 28.979873076898592 28.565972642151966 22.012469526361684 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 677.0 1 537.5 2 1423.0 3 1841.5 4 926.5 5 623.0 6 722.5 7 1123.5 8 1568.0 9 1669.5 10 1374.5 11 1062.0 12 1009.0 13 1020.5 14 1083.5 15 1146.0 16 1166.0 17 1177.5 18 1212.0 19 1295.5 20 1386.5 21 1539.0 22 1701.5 23 1860.5 24 2041.5 25 2288.0 26 2691.0 27 3107.5 28 3488.5 29 3919.0 30 4438.5 31 4859.5 32 5327.5 33 5873.0 34 6480.5 35 6905.5 36 7234.5 37 7730.5 38 7766.0 39 7495.5 40 7438.0 41 7582.5 42 7754.0 43 8156.5 44 8878.0 45 9201.0 46 9779.0 47 13946.0 48 26812.5 49 26101.5 50 14079.0 51 10293.5 52 7541.5 53 6284.5 54 5631.5 55 5035.0 56 4502.0 57 4049.0 58 3745.5 59 3430.0 60 2992.5 61 2578.5 62 2109.5 63 1507.5 64 1026.5 65 737.0 66 531.5 67 424.0 68 341.5 69 238.0 70 188.5 71 160.0 72 154.5 73 147.0 74 111.0 75 93.5 76 68.0 77 38.0 78 24.0 79 20.0 80 16.5 81 10.5 82 4.5 83 4.0 84 4.0 85 2.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03691575252443282 2 0.004773588688504244 3 0.0025459139672022635 4 0.0 5 9.547177377008488E-4 6 3.1823924590028294E-4 7 0.0 8 0.0 9 0.0019094354754016975 10-11 3.1823924590028294E-4 12-13 0.001432076606551273 14-15 0.0015911962295014144 16-17 0.00445534944260396 18-19 0.0 20-21 0.0 22-23 0.00222767472130198 24-25 0.001432076606551273 26-27 0.004773588688504244 28-29 0.0065239045409558 30-31 0.001432076606551273 32-33 0.0 34-35 0.008433340016357498 36-37 0.011774852098310468 38-39 0.007478622278656648 40-41 0.001750315852451556 42-43 3.1823924590028294E-4 44-45 0.002068555098351839 46-47 0.0 48-49 6.364784918005659E-4 50-51 0.001432076606551273 52-53 0.003500631704903112 54-55 0.0030232728360526876 56-57 0.001432076606551273 58-59 0.001432076606551273 60-61 0.003182392459002829 62-63 0.007478622278656648 64-65 0.01225221096716089 66-67 0.004137110196703678 68-69 0.004932708311454385 70-71 0.0 72-73 0.0019094354754016975 74-75 0.007478622278656648 76-77 0.004296229819653819 78-79 0.0055691868032549515 80-81 0.007001263409806224 82-83 0.006842143786856083 84-85 0.008433340016357498 86-87 0.005250947557354668 88-89 0.011456612852410184 90-91 0.016866680032714996 92-93 0.00222767472130198 94-95 0.0033415120819529705 96-97 0.0015911962295014144 98-99 0.0036597513278532534 100-101 0.007319502655706507 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 314229.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.03455124832343 #Duplication Level Percentage of deduplicated Percentage of total 1 89.31212112084582 55.40437354767579 2 6.844282593326648 8.491639985874599 3 1.5259552771015188 2.839858525200113 4 0.6626189709770729 1.6442108205275425 5 0.3882979057420255 1.2043943166685172 6 0.24089489749145346 0.8966284118335915 7 0.16350661609878622 0.710014168907407 8 0.13178627919354105 0.6540242152366066 9 0.10935932865190677 0.6105651189965059 >10 0.5233967173031727 5.83523378923501 >50 0.03890734182393631 1.6517277564628938 >100 0.04197897407319443 5.119689129386329 >500 0.0087029580395647 3.8601712900993013 >1k 0.008191019331355012 11.077468923895823 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4618 1.4696288375675066 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3562 1.1335681938968076 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3335 1.0613278850774435 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3319 1.0562360571430391 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3247 1.0333228314382188 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3177 1.011046084225199 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2039 0.6488898223906769 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1979 0.6297954676366598 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1932 0.6148382230793465 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1260 0.40098144983435646 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1151 0.36629337203122564 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1092 0.34751725652310894 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1065 0.3389247968838013 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1055 0.3357424044247985 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1029 0.3274681840313911 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1021 0.3249222700641889 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 884 0.2813234933758501 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 846 0.26923040203163934 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 845 0.2689121627857391 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 807 0.2568190714415283 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 785 0.2498178080317221 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 772 0.2456806978350184 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 771 0.24536245858911812 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 735 0.23390584573670792 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 717 0.22817753931050283 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 696 0.2214945151465969 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 683 0.21735740494989325 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 647 0.20590079209748305 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 625 0.19889952868767682 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 603 0.1918982652778706 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 589 0.18744291583526665 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 580 0.1845787626221641 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 570 0.18139637016316126 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 491 0.1562554697370389 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 417 0.13270576554041796 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 401 0.12761393760601344 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 399 0.12697745911421288 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 372 0.11838499947490524 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 372 0.11838499947490524 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 339 0.1078831043601959 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 333 0.10597366888479422 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 326 0.10374599416349223 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 326 0.10374599416349223 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 323 0.10279127642579139 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.5911962295014147E-4 0.0 16-17 0.0 0.0 0.0 6.364784918005659E-4 0.0 18-19 0.0 0.0 0.0 9.547177377008488E-4 0.0 20-21 0.0 0.0 0.0 0.0011138373606509903 0.0 22-23 0.0 0.0 0.0 0.001750315852451556 0.0 24-25 0.0 0.0 0.0 0.0022276747213019806 0.0 26-27 0.0 0.0 0.0 0.003341512081952971 0.0 28-29 0.0 0.0 0.0 0.004932708311454386 0.0 30-31 0.0 0.0 0.0 0.007796861524556931 0.0 32-33 0.0 0.0 0.0 0.01432076606551273 0.0 34-35 0.0 0.0 0.0 0.020208192114667962 0.0 36-37 0.0 0.0 0.0 0.024981780803172208 0.0 38-39 0.0 0.0 0.0 0.030073608737576737 0.0 40-41 0.0 0.0 0.0 0.03818870950803395 0.0 42-43 0.0 0.0 0.0 0.05441891104894838 0.0 44-45 0.0 0.0 0.0 0.07319502655706507 0.0 46-47 0.0 0.0 0.0 0.08847051036027864 0.0 48-49 0.0 0.0 0.0 0.11122461644214887 0.0 50-51 0.0 0.0 0.0 0.1377975934748225 0.0 52-53 0.0 0.0 0.0 0.18569259998281507 0.0 54-55 0.0 0.0 0.0 0.22881401780230343 0.0 56-57 0.0 0.0 0.0 0.2926209866053101 0.0 58-59 0.0 0.0 0.0 0.3861833248999933 0.0 60-61 0.0 0.0 0.0 0.4900884386864357 0.0 62-63 0.0 0.0 0.0 0.610701112882643 0.0 64-65 0.0 0.0 0.0 0.7074458436363289 0.0 66-67 0.0 0.0 0.0 0.8547906144881599 0.0 68-69 0.0 0.0 0.0 1.022343577454659 0.0 70-71 0.0 0.0 0.0 1.1614141279130825 0.0 72-73 0.0 0.0 0.0 1.3440834550598448 0.0 74-75 0.0 0.0 0.0 1.5413917875180203 0.0 76-77 0.0 0.0 0.0 1.682849132320696 0.0 78-79 0.0 0.0 0.0 1.8384681235659344 0.0 80-81 0.0 0.0 0.0 2.0889224100894572 0.0 82-83 0.0 0.0 0.0 2.3051659776786995 0.0 84-85 0.0 0.0 0.0 2.661275693841116 0.0 86-87 0.0 0.0 0.0 3.10474208300316 0.0 88-89 0.0 0.0 0.0 3.5891022152633907 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 30 1.3003855E-4 63.32718 2 TACCTGG 635 0.0 59.836704 2 TCACGCA 35 2.7861557E-4 54.28044 4 ATAACGC 35 2.7861557E-4 54.28044 3 GTACCTG 735 0.0 51.057583 1 ACCTGGG 750 0.0 48.761932 3 TATCACG 40 5.384746E-4 47.495388 2 CCTGGGA 520 0.0 47.495388 4 CTAGACG 50 0.0016147994 37.99631 3 TGGGACG 50 0.0016147994 37.99631 6 TACGTGG 65 1.3267467E-4 36.534912 2 TAAGGTG 80 1.0758355E-5 35.62154 5 ATCACGC 55 0.0025780138 34.542095 3 GTACGTG 70 2.049203E-4 33.93067 1 GTGTAGC 80 4.4846628E-4 29.68934 1 CCTATAC 65 0.005840467 29.22793 3 ACGTGGG 85 6.400377E-4 27.938461 3 GAACGCA 90 8.9380937E-4 26.386326 9 ATGGGAG 490 0.0 26.170927 5 GGGAACG 115 1.2797024E-4 24.780201 7 >>END_MODULE