FastQCFastQC Report
Thu 26 May 2016
SRR936731_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936731_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2129622
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT136040.638798810305303No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT88770.4168345368332972No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84390.3962675066279368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67390.31644113368475724No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC52020.24426870120612953No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT50210.23576954032217923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40530.19031546443453345No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT39440.18519718522817663No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT32490.15256228570140618No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC31940.14997966775324448No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC28390.1333100428151099No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG26710.1254213189007251No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC24510.11509084710807833No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA24100.11316562281944871No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA21990.10325776123650114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG17950.028.846232
GTACCTG18900.027.897661
GTAAGCG2200.024.83962494-95
GTACATG101250.024.3488771
TACATGG100400.023.8937682
ACATGGG103000.022.8299623
GAGTACT85350.022.42996212-13
GTATAGG6200.020.6861021
GTACTTT92400.020.6693614-15
AGTACTT89100.020.25969512-13
CATGGGG46900.019.6510834
GTGTAGC13200.019.0725381
AGAGTAC155300.018.40038510-11
CATGGGA54050.018.369964
ACTTTTT110250.017.67032416-17
GTACGCT1350.00939752117.594363
TATACCG1650.001462692517.2756795
ATGGGGA26700.017.081575
TACTTTT108750.016.60073314-15
GTATAGC8850.016.1021771
Produced by FastQC (version 0.11.5)