##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936731_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2129622 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.304407073180123 31.0 31.0 34.0 28.0 34.0 2 31.366441556294966 31.0 31.0 34.0 28.0 34.0 3 31.371712914310613 31.0 31.0 34.0 28.0 34.0 4 35.001873102362765 37.0 35.0 37.0 32.0 37.0 5 35.01478900950497 37.0 35.0 37.0 32.0 37.0 6 35.02136952003689 37.0 35.0 37.0 32.0 37.0 7 34.93255845403551 37.0 35.0 37.0 32.0 37.0 8 34.89075807819416 37.0 35.0 37.0 32.0 37.0 9 36.359953080875385 39.0 37.0 39.0 32.0 39.0 10-11 36.22872533247684 39.0 35.0 39.0 32.0 39.0 12-13 36.12811052853511 39.0 35.0 39.0 31.0 39.0 14-15 37.16181087535722 39.0 36.0 41.0 31.0 41.0 16-17 37.07257132955989 39.0 36.0 41.0 31.0 41.0 18-19 37.060560277833346 39.0 36.0 41.0 31.0 41.0 20-21 36.92283372354343 39.0 36.0 41.0 30.5 41.0 22-23 37.02178884327829 39.0 36.0 41.0 31.0 41.0 24-25 37.05074632963033 39.0 36.0 41.0 31.0 41.0 26-27 36.94727632415518 39.0 36.0 41.0 30.0 41.0 28-29 36.80342779141087 39.0 36.0 41.0 30.0 41.0 30-31 36.59008124446498 39.0 36.0 41.0 30.0 41.0 32-33 36.361412260016095 39.0 35.0 41.0 30.0 41.0 34-35 36.146277602316275 39.0 35.0 40.0 29.5 41.0 36-37 35.91825873323998 38.0 35.0 40.0 28.5 41.0 38-39 35.69962228038591 38.0 35.0 40.0 27.5 41.0 40-41 35.493745838463354 38.0 35.0 40.0 27.0 41.0 42-43 35.23387812485032 38.0 34.0 40.0 26.0 41.0 44-45 34.92846336110352 38.0 34.0 40.0 25.0 41.0 46-47 34.70245776010954 38.0 34.0 40.0 24.0 41.0 48-49 34.50077337668375 38.0 34.0 40.0 23.5 41.0 50-51 33.51955581788693 37.5 32.0 39.5 22.5 40.5 52-53 33.632300004413935 37.5 33.0 39.5 22.5 40.5 54-55 34.06952172733001 38.0 33.0 40.0 22.5 41.0 56-57 33.94485171546876 38.0 33.0 40.0 22.0 41.0 58-59 33.70232393354314 38.0 33.0 40.0 20.5 41.0 60-61 33.383098033359914 37.0 33.0 40.0 19.5 41.0 62-63 33.024308774045345 37.0 32.0 40.0 18.0 41.0 64-65 32.67691942513741 36.0 32.0 39.0 17.0 41.0 66-67 32.19275650796244 36.0 31.0 39.0 13.0 41.0 68-69 31.783063379322716 35.0 31.0 39.0 9.5 40.0 70-71 31.368072831704406 35.0 30.5 38.0 8.0 40.0 72-73 30.96382315734905 35.0 30.0 37.0 7.0 39.5 74-75 30.540734458979106 35.0 30.0 37.0 4.0 39.0 76-77 30.07577917583496 34.0 29.0 36.0 2.0 39.0 78-79 29.578388793879853 34.0 29.0 36.0 2.0 38.5 80-81 29.090537193924554 34.0 29.0 35.0 2.0 37.0 82-83 28.653574202370187 34.0 28.0 35.0 2.0 37.0 84-85 28.260024783740967 34.0 27.0 35.0 2.0 36.0 86-87 27.91330550679886 34.0 27.0 35.0 2.0 36.0 88-89 27.482321041011033 33.5 26.0 35.0 2.0 36.0 90-91 27.039335619185 33.0 25.5 35.0 2.0 35.0 92-93 26.56719643204287 33.0 25.0 35.0 2.0 35.0 94-95 26.04929607226071 33.0 24.0 35.0 2.0 35.0 96-97 25.430342098269083 32.0 21.0 35.0 2.0 35.0 98-99 24.668917066033316 32.0 10.0 35.0 2.0 35.0 100-101 21.824103995920403 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4805002023403375 1101 2 0.36070376250925307 1101 3 0.4814317989975194 1101 4 0.6213258930827195 1101 5 0.5040622278377569 1101 6 0.5922503167149245 1101 7 0.5125169637795324 1101 8 0.6161485863776051 1101 9 0.7215804508469361 1101 10-11 0.7892671386691603 1101 12-13 0.7902736831756201 1101 14-15 0.8602252755060391 1101 16-17 0.8715444815720943 1101 18-19 0.7525929990777982 1101 20-21 0.7729304012715303 1101 22-23 0.745843886764483 1101 24-25 0.7716518702930983 1101 26-27 0.6317428684314166 1101 28-29 0.8611164984744946 1101 30-31 0.7481491077207991 1101 32-33 0.5867887194695172 1101 34-35 0.5877187013856684 1101 36-37 0.6209180758716073 1101 38-39 0.7304733960557783 1101 40-41 0.6740353957359915 1101 42-43 0.7498128808475641 1101 44-45 0.7267149740906333 1101 46-47 0.8296705061803493 1101 48-49 0.853914713976117 1101 50-51 0.7057300509153706 1101 52-53 0.8057432452103157 1101 54-55 0.7704921344651225 1101 56-57 0.7531209870012674 1101 58-59 0.7002978611701209 1101 60-61 0.7933766080089057 1101 62-63 0.7758759709095528 1101 64-65 0.6603828376311967 1101 66-67 0.7533929984403116 1101 68-69 0.6488403422282012 1101 70-71 0.6214424189192798 1101 72-73 0.43923638232360673 1101 74-75 0.4749569678498169 1101 76-77 0.49949511405991487 1101 78-79 0.5905150737225142 1101 80-81 0.5724757213482548 1101 82-83 0.5077964995081636 1101 84-85 0.4100956607322601 1101 86-87 0.5824012066791333 1101 88-89 0.5672996307515312 1101 90-91 0.5137393832767891 1101 92-93 0.5080034542877137 1101 94-95 0.6624164363672413 1101 96-97 0.43958434566336635 1101 98-99 0.5704003919242489 1101 100-101 0.6060665798363267 1102 1 0.2757799658191402 1102 2 0.3178733112722405 1102 3 0.3524202373767267 1102 4 0.33126338143222966 1102 5 0.36944325845632875 1102 6 0.35332713263791504 1102 7 0.4606612665636547 1102 8 0.3703214485759716 1102 9 0.35659752378153087 1102 10-11 0.5830984482492241 1102 12-13 0.5526916601159613 1102 14-15 0.5701400047594944 1102 16-17 0.43091718308401283 1102 18-19 0.2982620153501898 1102 20-21 0.4270362144504176 1102 22-23 0.45755965527774123 1102 24-25 0.44363459216538814 1102 26-27 0.20167253921206907 1102 28-29 0.08465543675905707 1102 30-31 0.4715000125213109 1102 32-33 0.3460574516505872 1102 34-35 0.45616859741634386 1102 36-37 0.2328303312474489 1102 38-39 0.19368638795931759 1102 40-41 0.0861101438325349 1102 42-43 0.14568586197123778 1102 44-45 0.3346714818193348 1102 46-47 0.44727127117498355 1102 48-49 0.5562313370753671 1102 50-51 0.7122456549851321 1102 52-53 0.7506844761667324 1102 54-55 0.7851695838857395 1102 56-57 0.7264363081357317 1102 58-59 0.8277173698337563 1102 60-61 0.8453078076227953 1102 62-63 0.7150481322722655 1102 64-65 0.8936095644685835 1102 66-67 0.832051685695653 1102 68-69 0.797832222649749 1102 70-71 0.8521143469109411 1102 72-73 0.5914524680191917 1102 74-75 0.754676373483381 1102 76-77 0.7104332801247288 1102 78-79 0.8469190498449706 1102 80-81 0.99730640089874 1102 82-83 0.9162196770825766 1102 84-85 0.6103616170261255 1102 86-87 0.7676429082792033 1102 88-89 0.8708546738059617 1102 90-91 0.7426017825045719 1102 92-93 0.590921885687461 1102 94-95 0.45654090477978215 1102 96-97 0.5149500084732672 1102 98-99 0.6404097195410756 1102 100-101 1.0516167069260085 1103 1 0.4549054274504911 1103 2 0.31098149407297626 1103 3 0.367115340651889 1103 4 0.4650164223022273 1103 5 0.5218483181823643 1103 6 0.4389015270163057 1103 7 0.4876833791013695 1103 8 0.5539991498014771 1103 9 0.27206820292969525 1103 10-11 0.6089192631143661 1103 12-13 0.32487188029079306 1103 14-15 0.5443092443571729 1103 16-17 0.6007968156800345 1103 18-19 0.6583884204873698 1103 20-21 0.7060572919481629 1103 22-23 0.5453283168020704 1103 24-25 0.5516763070928405 1103 26-27 0.5277736774039852 1103 28-29 0.5388419798311546 1103 30-31 0.5167963895293397 1103 32-33 0.35054667350471647 1103 34-35 0.6111035201580037 1103 36-37 0.7009731033943112 1103 38-39 0.6566981430561825 1103 40-41 0.6615853292771945 1103 42-43 0.6338328059629035 1103 44-45 0.6862133041233847 1103 46-47 0.7517273119006376 1103 48-49 0.43515410337813165 1103 50-51 0.5618860314017056 1103 52-53 0.659549822394645 1103 54-55 0.7045401457997897 1103 56-57 0.6498624488800431 1103 58-59 0.6924534874480912 1103 60-61 0.6799168687992889 1103 62-63 0.8019302327697204 1103 64-65 0.7116084343739146 1103 66-67 0.8928697897418161 1103 68-69 0.7082891188676292 1103 70-71 0.7392963097551224 1103 72-73 0.5257592904816732 1103 74-75 0.5608448948900495 1103 76-77 0.7684937419497082 1103 78-79 0.5919281504515581 1103 80-81 0.6079234818747743 1103 82-83 0.4499794069864329 1103 84-85 0.5275594751270738 1103 86-87 0.4867193287307501 1103 88-89 0.5844404536240901 1103 90-91 0.6148992367991077 1103 92-93 0.5858699928669076 1103 94-95 0.6142454395926826 1103 96-97 0.6698866034733904 1103 98-99 0.6005760828541895 1103 100-101 0.6193787322246394 1104 1 0.3578673328154167 1104 2 0.3907274049884073 1104 3 0.26412578976674084 1104 4 0.4107074220801721 1104 5 0.40650817572767295 1104 6 0.4757112696543473 1104 7 0.5250845575139635 1104 8 0.4991190466390947 1104 9 0.5221135505007695 1104 10-11 0.6434486070505017 1104 12-13 0.5880904966057514 1104 14-15 0.4862164642077218 1104 16-17 0.46060138860578803 1104 18-19 0.5106000175155714 1104 20-21 0.3712551491528089 1104 22-23 0.5175881775578262 1104 24-25 0.42147651916074125 1104 26-27 0.3083274617635823 1104 28-29 0.31720971697397715 1104 30-31 0.575663422603192 1104 32-33 0.4829127108939488 1104 34-35 0.49771265441624024 1104 36-37 0.45969807416586406 1104 38-39 0.451439340856318 1104 40-41 0.2661585225755658 1104 42-43 0.41528506972969836 1104 44-45 0.5485298738672384 1104 46-47 0.2456324556618057 1104 48-49 0.47064693828354365 1104 50-51 0.1684785961836326 1104 52-53 0.2044629129051856 1104 54-55 0.06862950682263858 1104 56-57 0.13647523948127827 1104 58-59 0.24047607472990506 1104 60-61 0.25433869841548784 1104 62-63 0.4283244810746467 1104 64-65 0.3575263949549665 1104 66-67 0.41155245394951834 1104 68-69 0.5255615048276248 1104 70-71 0.39354359804171324 1104 72-73 0.4597898557255462 1104 74-75 0.5037341549621566 1104 76-77 0.32242185824638625 1104 78-79 0.26343186163970245 1104 80-81 0.5019074599581188 1104 82-83 0.47268957759496644 1104 84-85 0.3392612887623798 1104 86-87 0.35320038396917397 1104 88-89 0.21617019815272798 1104 90-91 0.06028979219761155 1104 92-93 -0.10607535104782428 1104 94-95 -0.09733375278357315 1104 96-97 0.14403331557383936 1104 98-99 0.33481657569355505 1104 100-101 0.2594526081889974 1105 1 0.2571909362927478 1105 2 0.05573432425510205 1105 3 0.20891363090577997 1105 4 0.12607697204708757 1105 5 0.1659838751351117 1105 6 0.23943039504766972 1105 7 0.26593101091890503 1105 8 0.34923447436491983 1105 9 0.3781860510533903 1105 10-11 0.31521895847248516 1105 12-13 0.20121028983090383 1105 14-15 0.1869531155669435 1105 16-17 0.13831744066482798 1105 18-19 0.22053987995471402 1105 20-21 0.05258196029840434 1105 22-23 -0.015581301475059206 1105 24-25 -0.08321708561728514 1105 26-27 -0.135792352073679 1105 28-29 -0.15442333302598144 1105 30-31 -0.22580675276307716 1105 32-33 -0.468218502141994 1105 34-35 -0.4216579872808879 1105 36-37 -0.2040237876606099 1105 38-39 -0.29081912982582736 1105 40-41 -0.34713665113819303 1105 42-43 -0.35763140295820506 1105 44-45 -0.15111913415797318 1105 46-47 -0.09309603401899835 1105 48-49 -0.0786138338454947 1105 50-51 -0.019751045286760416 1105 52-53 0.05367478461835873 1105 54-55 0.022528693071386385 1105 56-57 -0.2024434678419169 1105 58-59 -0.058823611610890225 1105 60-61 -0.03352749497855001 1105 62-63 -0.06051353700855344 1105 64-65 -0.1363598092284306 1105 66-67 -0.035287178984624745 1105 68-69 -0.07389575741720122 1105 70-71 -0.14794668122372556 1105 72-73 -0.18008274731893437 1105 74-75 -0.08882258693391165 1105 76-77 -0.12395951097947133 1105 78-79 -0.08877693744995696 1105 80-81 0.13819826746177455 1105 82-83 -0.0834485262771949 1105 84-85 -0.29174820275147795 1105 86-87 -0.4920959895105952 1105 88-89 -0.42123615008788917 1105 90-91 -0.33848812386283456 1105 92-93 -0.304427840597846 1105 94-95 -0.19979318427359516 1105 96-97 -0.14895542737984613 1105 98-99 -0.009265638841384316 1105 100-101 -0.15838145521522407 1106 1 0.2359633331751354 1106 2 0.24507664845161514 1106 3 0.32628905782582507 1106 4 0.37973963953110257 1106 5 0.2548991917127452 1106 6 0.32388735274974323 1106 7 0.5019455480937864 1106 8 0.5199914583389997 1106 9 0.4930672140273771 1106 10-11 0.48894783140356424 1106 12-13 0.45425898060943126 1106 14-15 0.5910078489061235 1106 16-17 0.49838784963234417 1106 18-19 0.4194063306071385 1106 20-21 0.15700905549062583 1106 22-23 0.27347027922377976 1106 24-25 0.3529944040052655 1106 26-27 0.28808968663373946 1106 28-29 0.2888628566578717 1106 30-31 0.40226348105775145 1106 32-33 0.20356326508805722 1106 34-35 0.2243280009842863 1106 36-37 0.17207254301633412 1106 38-39 0.10840015382422763 1106 40-41 0.12628019797293888 1106 42-43 0.2404105446729332 1106 44-45 0.13526329862948216 1106 46-47 0.1492761526873636 1106 48-49 -0.06421607572306698 1106 50-51 -0.11745382136172822 1106 52-53 -0.03434623298388573 1106 54-55 0.11867397724688544 1106 56-57 0.1458562957100611 1106 58-59 0.2090103493314004 1106 60-61 0.4027662260127798 1106 62-63 0.27799088544525574 1106 64-65 0.23232483916459756 1106 66-67 0.18303526606310072 1106 68-69 0.43885982538339263 1106 70-71 0.32854276618848033 1106 72-73 0.09230362626895072 1106 74-75 0.24217881423957266 1106 76-77 0.11184590105310477 1106 78-79 0.017205372734828472 1106 80-81 0.25116971855206316 1106 82-83 0.12463274178480432 1106 84-85 0.20617061675985937 1106 86-87 0.2279095276099916 1106 88-89 0.18984073930698386 1106 90-91 -0.030323485978609455 1106 92-93 -0.07445656465424477 1106 94-95 0.10208062253961003 1106 96-97 0.08756729790257722 1106 98-99 0.18708789474299437 1106 100-101 -0.07759390814455003 1107 1 0.13192764778384358 1107 2 0.20566965568927742 1107 3 0.3706788857400234 1107 4 0.2846755064106006 1107 5 0.24100183094481054 1107 6 0.3123104170577946 1107 7 0.3945701299056097 1107 8 0.46038093639641886 1107 9 0.4068735245690007 1107 10-11 0.5635346724155283 1107 12-13 0.2954258404261978 1107 14-15 0.10083375142728102 1107 16-17 0.26383494472778324 1107 18-19 0.3538805177367834 1107 20-21 0.472112022713425 1107 22-23 0.3023277781851448 1107 24-25 0.24865250646957548 1107 26-27 0.24798542931408463 1107 28-29 0.3277992920546282 1107 30-31 0.3143491085040111 1107 32-33 0.10657133365999272 1107 34-35 0.27277992847646715 1107 36-37 0.21122790129153657 1107 38-39 0.2824665828798345 1107 40-41 0.29025579546271985 1107 42-43 0.2859004097594138 1107 44-45 0.2140087996899922 1107 46-47 0.35222290885905494 1107 48-49 0.3959777449316064 1107 50-51 0.19635187693054235 1107 52-53 0.3443449528119302 1107 54-55 0.4917272210751946 1107 56-57 0.2135968769096337 1107 58-59 0.31538928795340126 1107 60-61 0.38425407942279577 1107 62-63 0.4169753386847077 1107 64-65 0.376950820403529 1107 66-67 0.2109479767971436 1107 68-69 0.28321090665949455 1107 70-71 0.34271292777858164 1107 72-73 0.23322681084026442 1107 74-75 0.24867171870680593 1107 76-77 0.25217599092256293 1107 78-79 0.2482555115842331 1107 80-81 0.24143993918049134 1107 82-83 0.2335352848062584 1107 84-85 -0.0801766598237883 1107 86-87 0.17404960151723614 1107 88-89 0.011862386695081995 1107 90-91 0.03232440504100609 1107 92-93 0.20862252484349497 1107 94-95 0.25601314391367325 1107 96-97 0.09739180099662192 1107 98-99 0.029292604689860724 1107 100-101 -0.2746029825547147 1108 1 0.21458317670287386 1108 2 0.13059407377167176 1108 3 0.20619926408471656 1108 4 0.20337424123807324 1108 5 0.23616181617931176 1108 6 0.11313539684647367 1108 7 0.28171973017060026 1108 8 0.16665237583544013 1108 9 0.10681401345838992 1108 10-11 0.23567880579901868 1108 12-13 0.27096452826234696 1108 14-15 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44-45 -1.6104383997058136 2316 46-47 -1.8446575954209266 2316 48-49 -1.927256936661358 2316 50-51 -1.5307237053368326 2316 52-53 -1.5132169240886455 2316 54-55 -1.439507290619268 2316 56-57 -1.6840713989258944 2316 58-59 -1.3349358420255868 2316 60-61 -1.6628372715490123 2316 62-63 -1.836741921011786 2316 64-65 -1.8694710242959722 2316 66-67 -1.46196422948087 2316 68-69 -1.461279404480404 2316 70-71 -1.3320430754123258 2316 72-73 -1.3879620539059303 2316 74-75 -1.1977976444791132 2316 76-77 -1.2480256308117 2316 78-79 -1.9576653183699335 2316 80-81 -1.9298035452784426 2316 82-83 -1.988187167413109 2316 84-85 -1.7726893126162935 2316 86-87 -1.8801758936107902 2316 88-89 -2.160277976011667 2316 90-91 -2.2790398135387235 2316 92-93 -2.825590918109768 2316 94-95 -2.698530778166628 2316 96-97 -2.951421099608776 2316 98-99 -3.9659923137655007 2316 100-101 -4.906573898055431 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5348.0 3 2193.0 4 2330.0 5 2507.0 6 2721.0 7 3355.0 8 4245.0 9 5493.0 10 6756.0 11 8655.0 12 10775.0 13 12931.0 14 14584.0 15 17077.0 16 20070.0 17 24847.0 18 20394.0 19 15966.0 20 15919.0 21 17075.0 22 18921.0 23 21340.0 24 23954.0 25 27358.0 26 31711.0 27 37232.0 28 43406.0 29 51809.0 30 62332.0 31 76242.0 32 94084.0 33 118184.0 34 152828.0 35 205020.0 36 279888.0 37 357935.0 38 273278.0 39 40854.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.90725673707707 18.507513714130873 14.521269876156031 22.06395967263603 2 15.335742181262832 20.405828572931362 40.35401047820672 23.904418767599086 3 20.348940966514817 24.560653735969566 29.610247499178133 25.480157798337483 4 12.48989080662205 16.512950569449337 38.20025830691558 32.79690031701303 5 13.36006925411384 37.70676068391726 34.11981656866158 14.81335349330733 6 30.845575281521743 36.27160447428134 17.777000014093705 15.105820230103213 7 28.088334807082827 32.9283692003105 20.85871305275585 18.12458293985083 8 26.027625674366874 33.47057867558714 19.87859282660587 20.623202823440113 9 25.96917431468466 17.58394343676516 21.165930909955215 35.28095133859496 10-11 24.968673275949744 26.382280688856753 27.16878991588349 21.480256119310013 12-13 26.70469290644742 23.969236745400412 27.392185464463186 21.93388488368898 14-15 23.074231328265416 25.487109405365445 27.02852753164787 24.41013173472127 16-17 21.565349194293475 28.64420918543486 27.46682599021795 22.32361563005372 18-19 21.335341377251034 27.685572824430565 29.656003101774996 21.323082696543405 20-21 22.77491658374372 26.040013493184343 29.40908121374764 21.7759887093243 22-23 21.94548030246924 26.13769380707313 29.65908928139957 22.257736609058064 24-25 22.361498093608194 26.22025393669497 29.764013136130156 21.654234833566676 26-27 21.972675555680777 25.9597951723215 30.431067879103775 21.636461392893956 28-29 21.312187458839478 27.241110442499235 29.837888881088855 21.608813217572433 30-31 21.79188273252819 26.268725121022708 29.59807640924717 22.341315737201928 32-33 21.74924674096459 26.359804834710328 29.80412330227634 22.086825122048744 34-35 22.703301337044792 26.2105904240283 29.465510780786445 21.62059745814046 36-37 22.315496710323195 25.948850415086472 30.01469982975808 21.72095304483225 38-39 21.878822569837546 26.66404022124124 29.830237399048936 21.626899809872274 40-41 22.04850325579255 26.751640809564663 29.447912249734575 21.751943684908216 42-43 21.680409853771238 26.798839185927477 28.843294796059244 22.677456164242045 44-45 22.137463904343782 26.441214236861697 28.904722963564016 22.5165988952305 46-47 22.799054189246203 27.02646481043519 28.426656348617335 21.74782465170127 48-49 22.44569835253757 26.92349321483268 28.474693822831753 22.156114609797996 50-51 21.778938073085925 27.348629477475008 28.882509299567584 21.989923149871483 52-53 22.40194051616396 27.649303029086376 27.79926523917294 22.149491215576724 54-55 21.93479690068091 27.10577600375675 28.20392110824137 22.755505987320966 56-57 22.505093658037694 27.168015349296514 27.822008641022833 22.504882351642955 58-59 23.084395070088362 26.832099039652913 27.82752494082505 22.255980949433678 60-61 22.340966048871106 27.171233883415407 28.769896706410382 21.717903361303105 62-63 21.998836872685455 27.308017666199362 28.620116373775684 22.0730290873395 64-65 21.793466993707508 27.90571381547205 28.04052777010159 22.260291420718854 66-67 22.11826959858935 27.36996052795649 27.588842625392253 22.922927248061917 68-69 21.96750952980352 27.316930178349253 28.014885965322296 22.70067432652493 70-71 22.614008509866593 27.105073807694303 27.76150761779409 22.51941006464501 72-73 22.35216350951841 26.82413662738136 28.48013074750093 22.343569115599294 74-75 21.795699518189153 28.20668428883286 27.639432160727306 22.358184032250676 76-77 22.185818118878824 27.299335590539087 27.63455812226534 22.88028816831675 78-79 22.267443963801934 27.48279107179521 27.731052560114378 22.518712404288475 80-81 22.65550800244183 27.390546512004953 27.4565031559091 22.497442329644116 82-83 22.464159783555182 27.309174968564715 27.94910878358079 22.277556464299312 84-85 22.258526443139747 27.700237973463633 27.700731109711548 22.340504473685073 86-87 22.19641926196593 27.816543535743975 27.557663314812814 22.42937388747728 88-89 22.092194767516954 27.727402315413435 27.09751855576216 23.08288436130745 90-91 22.295652059539 27.221291724710216 27.440101522484962 23.04295469326582 92-93 22.664446283021885 27.498266254873332 27.602208766632625 22.23507869547216 94-95 22.22918321140905 27.33868494599467 27.7745033994309 22.657628443165375 96-97 21.682593611406347 27.420755485273585 28.25926251208787 22.637388391232204 98-99 21.911534708516303 27.762114988623665 27.293014322956427 23.033335979903605 100-101 21.950466534814254 27.22461006974645 27.52139547406957 23.303527921369724 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 246.0 1 210.5 2 319.0 3 456.0 4 388.0 5 361.5 6 601.5 7 1171.0 8 1808.0 9 2171.0 10 2098.0 11 1959.0 12 2048.5 13 2228.5 14 2378.0 15 2545.0 16 2764.5 17 2995.0 18 3258.5 19 3626.5 20 4028.0 21 4568.0 22 5525.5 23 6804.0 24 8256.5 25 10166.5 26 13068.0 27 16656.5 28 19776.5 29 22820.5 30 26747.0 31 31220.5 32 36084.5 33 39939.0 34 43634.5 35 48089.5 36 53009.5 37 56886.0 38 58187.5 39 59299.0 40 60896.5 41 62447.5 42 63625.0 43 65602.5 44 68456.0 45 71788.5 46 74565.5 47 83970.0 48 105085.0 49 104262.5 50 83291.0 51 73282.0 52 69455.0 53 67371.5 54 65532.0 55 60829.5 56 54796.5 57 49319.5 58 45835.5 59 41438.0 60 34563.5 61 28645.5 62 23645.0 63 18265.5 64 12938.0 65 9476.5 66 7230.5 67 5681.5 68 4414.5 69 3374.5 70 2689.0 71 2069.5 72 1620.5 73 1386.5 74 1074.0 75 767.0 76 550.5 77 357.0 78 240.0 79 170.5 80 110.0 81 69.5 82 50.5 83 33.0 84 19.0 85 10.0 86 7.5 87 7.5 88 8.0 89 5.5 90 3.5 91 2.5 92 1.5 93 1.5 94 1.0 95 1.0 96 1.0 97 0.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011692215801677482 2 0.011739172491644058 3 0.015120054169237547 4 0.017467888667566357 5 0.030568805168241127 6 0.04752017024617514 7 0.006245239765554638 8 0.027610533700346822 9 0.03512360409499902 10-11 0.03984275143663993 12-13 0.030686196893157568 14-15 0.028573145844661633 16-17 0.029160104469243837 18-19 0.024347043747669776 20-21 0.0535306265618969 22-23 0.013218308225591209 24-25 0.003545230092476505 26-27 0.0012678306290975583 28-29 1.1739172491644058E-4 30-31 0.0029582714678943025 32-33 0.0019956593235794895 34-35 0.0 36-37 2.5826179481616923E-4 38-39 4.695668996657623E-5 40-41 0.0015495707688970156 42-43 0.003850448577259251 44-45 0.0017608758737466086 46-47 9.626121443148128E-4 48-49 6.339153145487791E-4 50-51 0.004108710372075421 52-53 0.004014796992142268 54-55 0.005728716175922299 56-57 6.10436969565491E-4 58-59 0.0061748047306047745 60-61 0.0028643580879611497 62-63 8.686987643816602E-4 64-65 9.626121443148128E-4 66-67 0.018477457501847747 68-69 0.042519282764734774 70-71 0.05407062849651252 72-73 0.015354837619070425 74-75 0.031672287382455665 76-77 0.02763401204533011 78-79 0.05733411844918957 80-81 0.08024898315287878 82-83 0.04895234929015572 84-85 0.018453979156864458 86-87 0.021224423864892455 88-89 0.023431388293321538 90-91 0.039091444397174706 92-93 0.026319224726265977 94-95 0.013852223540139987 96-97 0.019768766475928592 98-99 0.050760181853868894 100-101 0.0697306846003657 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2129622.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.422002470860114 #Duplication Level Percentage of deduplicated Percentage of total 1 81.5982492821275 50.11927869024695 2 11.294016113631995 13.874021712748766 3 3.215975977862006 5.925950533754007 4 1.2891765725151594 3.1673522648960435 5 0.6624896605862496 2.0345720784723946 6 0.39825596575367056 1.4677007347534041 7 0.2710469991821241 1.1653774617438553 8 0.20487958521248137 1.006729151131986 9 0.14464843589780876 0.7996136928409135 >10 0.8235346004513073 9.46103888221574 >50 0.061492367495823305 2.5836463038854425 >100 0.032330169815214235 3.4502056724093353 >500 0.0019138575826128366 0.7573702439851594 >1k 0.0015310860660902252 1.9356741456442752 >5k 3.827715165225563E-4 1.6117922160130804 >10k+ 7.655430330451125E-5 0.6396762152588233 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13604 0.638798810305303 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8877 0.4168345368332972 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8439 0.3962675066279368 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6739 0.31644113368475724 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5202 0.24426870120612953 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5021 0.23576954032217923 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4053 0.19031546443453345 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3944 0.18519718522817663 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3249 0.15256228570140618 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3194 0.14997966775324448 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2839 0.1333100428151099 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2671 0.1254213189007251 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2451 0.11509084710807833 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2410 0.11316562281944871 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2199 0.10325776123650114 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.6956689966576225E-5 0.0 8 0.0 0.0 0.0 4.6956689966576225E-5 0.0 9 0.0 0.0 0.0 4.6956689966576225E-5 0.0 10-11 0.0 0.0 0.0 4.6956689966576225E-5 0.0 12-13 0.0 0.0 0.0 4.6956689966576225E-5 0.0 14-15 0.0 0.0 0.0 4.6956689966576225E-5 0.0 16-17 0.0 0.0 0.0 4.6956689966576225E-5 0.0 18-19 0.0 0.0 0.0 4.6956689966576225E-5 0.0 20-21 0.0 0.0 0.0 9.391337993315245E-5 0.0 22-23 0.0 0.0 0.0 1.408700698997287E-4 2.3478344983288113E-5 24-25 0.0 0.0 0.0 2.5826179481616923E-4 4.6956689966576225E-5 26-27 0.0 0.0 0.0 3.052184847827455E-4 4.6956689966576225E-5 28-29 0.0 0.0 0.0 5.165235896323385E-4 4.6956689966576225E-5 30-31 0.0 0.0 0.0 0.0011269605591978295 4.6956689966576225E-5 32-33 0.0 0.0 0.0 0.0018782675986630491 4.6956689966576225E-5 34-35 0.0 0.0 0.0 0.0030052281578608784 4.6956689966576225E-5 36-37 0.0 0.0 0.0 0.003967840302175691 4.6956689966576225E-5 38-39 0.0 0.0 0.0 0.005259149276256538 9.391337993315245E-5 40-41 0.0 0.0 0.0 0.007113938529936298 9.391337993315245E-5 42-43 0.0 0.0 0.0 0.010142645032780465 9.391337993315245E-5 44-45 0.0 0.0 0.0 0.014603530579605206 9.391337993315245E-5 46-47 0.0 0.0 0.0 0.024863567337302113 9.391337993315245E-5 48-49 0.0 0.0 0.0 0.04507842236791318 1.408700698997287E-4 50-51 0.0 0.0 0.0 0.06174804730604774 1.408700698997287E-4 52-53 0.0 0.0 0.0 0.07806549706943297 1.408700698997287E-4 54-55 0.0 4.6956689966576225E-5 0.0 0.10163775543265424 1.408700698997287E-4 56-57 0.0 4.6956689966576225E-5 0.0 0.12016216962446857 1.408700698997287E-4 58-59 0.0 4.6956689966576225E-5 0.0 0.14434486495725535 1.408700698997287E-4 60-61 0.0 4.6956689966576225E-5 0.0 0.18524414191814323 1.408700698997287E-4 62-63 0.0 4.6956689966576225E-5 0.0 0.21423989797250403 1.878267598663049E-4 64-65 0.0 4.6956689966576225E-5 0.0 0.24804871474843893 1.878267598663049E-4 66-67 0.0 4.6956689966576225E-5 0.0 0.3101254588842527 1.878267598663049E-4 68-69 0.0 4.6956689966576225E-5 0.0 0.38490398765602535 1.878267598663049E-4 70-71 0.0 4.6956689966576225E-5 0.0 0.4489529127704353 2.1130510484959302E-4 72-73 0.0 4.6956689966576225E-5 0.0 0.5172044616368539 2.5826179481616923E-4 74-75 0.0 4.6956689966576225E-5 0.0 0.5741629265663108 2.817401397994574E-4 76-77 0.0 4.6956689966576225E-5 0.0 0.6225987522668343 2.817401397994574E-4 78-79 0.0 4.6956689966576225E-5 0.0 0.6899346456789046 2.817401397994574E-4 80-81 0.0 4.6956689966576225E-5 0.0 0.7874167340495168 3.052184847827455E-4 82-83 0.0 4.6956689966576225E-5 0.0 0.899079742790035 3.521751747493217E-4 84-85 0.0 4.6956689966576225E-5 0.0 1.0313802167708634 3.756535197326098E-4 86-87 0.0 4.6956689966576225E-5 0.0 1.2425209732055738 3.756535197326098E-4 88-89 0.0 4.6956689966576225E-5 0.0 1.5043514764592025 3.756535197326098E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1795 0.0 28.84623 2 GTACCTG 1890 0.0 27.89766 1 GTAAGCG 220 0.0 24.839624 94-95 GTACATG 10125 0.0 24.348877 1 TACATGG 10040 0.0 23.893768 2 ACATGGG 10300 0.0 22.829962 3 GAGTACT 8535 0.0 22.429962 12-13 GTATAGG 620 0.0 20.686102 1 GTACTTT 9240 0.0 20.66936 14-15 AGTACTT 8910 0.0 20.259695 12-13 CATGGGG 4690 0.0 19.651083 4 GTGTAGC 1320 0.0 19.072538 1 AGAGTAC 15530 0.0 18.400385 10-11 CATGGGA 5405 0.0 18.36996 4 ACTTTTT 11025 0.0 17.670324 16-17 GTACGCT 135 0.009397521 17.59436 3 TATACCG 165 0.0014626925 17.275679 5 ATGGGGA 2670 0.0 17.08157 5 TACTTTT 10875 0.0 16.600733 14-15 GTATAGC 885 0.0 16.102177 1 >>END_MODULE