FastQCFastQC Report
Thu 26 May 2016
SRR936731_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936731_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2129622
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT157780.7408826542926397No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92770.4356172128199277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83920.39406054219950765No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT79080.3713335042556848No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50080.23515910335261378No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT48210.226378202328864No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC46320.2175033879251811No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40050.18806154331613778No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC38830.1823328271402155No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC36420.17101626485827062No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT29660.1392735424408651No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG28920.13579874738333847No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA27450.12889611395825176No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA26030.12222826398299792No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24600.11551345731777751No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC23050.10823517037295821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG19550.025.0273442
GTACCTG20700.025.0150221
GAGTACT107750.021.88824712-13
GTACATG95300.021.6840841
TACATGG93950.021.0339532
GTACTTT113000.020.59807614-15
AGTACTT112350.019.99848412-13
ACATGGG94700.019.9625783
TATACCG1559.6296944E-418.3866715
AGAGTAC174800.018.16619310-11
ACTTTTT131900.017.80758316-17
TATACGA1350.0093991317.5938362
CTAGACG1350.00940421517.5921844
CATGGGG44350.017.4573174
TACTTTT128700.017.34715714-15
GTATAGC8400.016.9662821
TAGACTC8450.016.3013975
TCTAGAC9150.016.0925243
AGTACAT76200.016.0214732
ATACCGT1900.00375766214.9985956
Produced by FastQC (version 0.11.5)