##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936731_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2129622 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.852020217672433 31.0 31.0 34.0 30.0 34.0 2 31.968229103568614 31.0 31.0 34.0 30.0 34.0 3 32.084805660347236 33.0 31.0 34.0 30.0 34.0 4 35.82218581513527 37.0 35.0 37.0 35.0 37.0 5 35.685538560364236 37.0 35.0 37.0 33.0 37.0 6 35.734921502501386 37.0 35.0 37.0 33.0 37.0 7 35.66725926009404 37.0 35.0 37.0 33.0 37.0 8 35.633360756040275 37.0 35.0 37.0 33.0 37.0 9 37.22882558500992 39.0 37.0 39.0 33.0 39.0 10-11 37.12306291914716 39.0 37.0 39.0 33.0 39.0 12-13 37.000944299035226 39.0 37.0 39.0 33.0 39.0 14-15 38.04947521203293 40.0 37.0 41.0 33.0 41.0 16-17 38.0456508244186 40.0 37.0 41.0 33.0 41.0 18-19 38.217789119383625 40.0 37.5 41.0 33.0 41.0 20-21 38.22982505815586 40.0 38.0 41.0 33.0 41.0 22-23 38.12378652173954 40.0 37.0 41.0 33.0 41.0 24-25 38.0020423812301 40.0 37.0 41.0 33.0 41.0 26-27 37.783809990693186 40.0 37.0 41.0 32.5 41.0 28-29 37.80149904537049 40.0 37.0 41.0 33.0 41.0 30-31 37.66915983212044 40.0 37.0 41.0 33.0 41.0 32-33 37.45451117616178 39.0 36.0 41.0 32.0 41.0 34-35 37.24576028046292 39.0 36.0 41.0 31.5 41.0 36-37 37.04652257536783 39.0 36.0 41.0 31.0 41.0 38-39 36.83254375659155 39.0 35.0 41.0 31.0 41.0 40-41 36.6236346168475 39.0 35.0 40.5 30.0 41.0 42-43 36.34158620637841 39.0 35.0 40.0 30.0 41.0 44-45 36.03117572038606 39.0 35.0 40.0 29.5 41.0 46-47 35.63323209470977 38.0 35.0 40.0 28.5 41.0 48-49 35.20234529883707 38.0 34.5 40.0 27.0 41.0 50-51 34.876429948601206 38.0 34.0 40.0 26.0 41.0 52-53 34.620327222389705 38.0 34.0 40.0 25.0 41.0 54-55 34.29743494385389 38.0 33.0 40.0 24.0 41.0 56-57 33.93762742871739 37.5 33.0 40.0 23.0 41.0 58-59 33.567303728079445 37.0 33.0 40.0 22.5 41.0 60-61 33.157753817344116 36.5 32.0 39.0 22.0 40.5 62-63 32.835924638269134 36.0 32.0 39.0 21.0 40.0 64-65 32.52151532055923 36.0 31.0 39.0 20.0 40.0 66-67 32.053218599357066 35.0 31.0 38.5 18.5 40.0 68-69 31.584721842655647 35.0 30.5 38.0 17.5 40.0 70-71 31.053940558465307 34.5 30.0 37.5 13.5 39.5 72-73 30.47417335095148 34.0 29.0 37.0 9.0 39.0 74-75 29.9355157863696 34.0 29.0 36.0 7.5 39.0 76-77 28.568793429068634 32.0 27.0 34.5 7.0 36.5 78-79 29.34608512684411 34.0 29.0 35.0 6.0 37.0 80-81 29.167720140006068 34.0 29.0 35.0 2.0 37.0 82-83 28.730457799553157 33.5 28.5 35.0 2.0 36.5 84-85 28.268972146230645 33.0 27.0 35.0 2.0 36.0 86-87 27.846330945116083 33.0 26.5 35.0 2.0 35.5 88-89 27.315224485847722 32.5 26.0 35.0 2.0 35.0 90-91 26.69765221245836 32.0 25.0 34.0 2.0 35.0 92-93 26.084077831652756 32.0 24.5 34.0 2.0 35.0 94-95 25.303412530486632 31.0 21.5 34.0 2.0 35.0 96-97 24.43049071619283 31.0 17.0 34.0 2.0 35.0 98-99 23.270404090491176 31.0 2.0 34.0 2.0 35.0 100-101 19.499442624090097 25.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31382899400718856 1101 2 0.3694483583804882 1101 3 0.34268955196295536 1101 4 0.27183261406545967 1101 5 0.2858687778553417 1101 6 0.31742433995988506 1101 7 0.31282748026384155 1101 8 0.2848734972041882 1101 9 0.3432712536218929 1101 10-11 0.2551998672194955 1101 12-13 0.3847829772449174 1101 14-15 0.3827607314625894 1101 16-17 0.36926195995216204 1101 18-19 0.4861007949891487 1101 20-21 0.342839604058355 1101 22-23 0.38682897297887564 1101 24-25 0.3934816377224095 1101 26-27 0.28923516188246623 1101 28-29 0.3991169857920909 1101 30-31 0.4175935215348616 1101 32-33 0.3531392465766743 1101 34-35 0.5614096591841999 1101 36-37 0.4943776936837523 1101 38-39 0.5417003146992769 1101 40-41 0.5957481492515413 1101 42-43 0.6417800582001192 1101 44-45 0.7153302049484722 1101 46-47 0.5533261582342206 1101 48-49 0.4960605507811948 1101 50-51 0.5238962168791588 1101 52-53 0.5258695283946295 1101 54-55 0.5654261805162548 1101 56-57 0.4436170212394259 1101 58-59 0.5079387244679836 1101 60-61 0.5413849992758699 1101 62-63 0.2587695371659109 1101 64-65 0.4581397904124884 1101 66-67 0.44393707766403523 1101 68-69 0.4380821400309607 1101 70-71 0.3255494311438021 1101 72-73 0.44042764851603167 1101 74-75 0.5108258686639751 1101 76-77 0.5265738885713773 1101 78-79 0.6386228245464913 1101 80-81 0.7507299997104155 1101 82-83 0.7299974852959714 1101 84-85 0.7187850937334481 1101 86-87 0.7624485756708559 1101 88-89 0.5430658066152709 1101 90-91 0.7940114505833762 1101 92-93 0.6662653853856639 1101 94-95 0.4583065811907723 1101 96-97 0.4380169359028194 1101 98-99 -0.14880564851433675 1101 100-101 0.18110397136572942 1102 1 0.31678081263959257 1102 2 0.43670726730219656 1102 3 0.32534495323832857 1102 4 0.23901941639078927 1102 5 0.09748993355501057 1102 6 0.1284266650002408 1102 7 0.23037235903372277 1102 8 0.16667395123008788 1102 9 0.3367993614667668 1102 10-11 0.39562674116011465 1102 12-13 0.39893823185055766 1102 14-15 0.5564559849989195 1102 16-17 0.5164748376989721 1102 18-19 0.4496799193347343 1102 20-21 0.3920012384360021 1102 22-23 0.29322875853760877 1102 24-25 0.3034484600179397 1102 26-27 0.33405251833035976 1102 28-29 0.4914011464727537 1102 30-31 0.6229250023500228 1102 32-33 0.5667725592461892 1102 34-35 0.5402494799436752 1102 36-37 0.5571595632820845 1102 38-39 0.5665310303753088 1102 40-41 0.6324620031106818 1102 42-43 0.595020050339194 1102 44-45 0.7403475090865754 1102 46-47 0.6326177651131033 1102 48-49 0.867387124301743 1102 50-51 0.8067527652129698 1102 52-53 0.8797700400768989 1102 54-55 0.9699841049444871 1102 56-57 0.8657373814545011 1102 58-59 0.9541954673017585 1102 60-61 0.8068819567821137 1102 62-63 0.783104790933443 1102 64-65 0.8070291465251458 1102 66-67 0.8002433703012208 1102 68-69 0.6123145804852221 1102 70-71 0.3619041970430139 1102 72-73 0.40482122337033744 1102 74-75 0.6938583122972766 1102 76-77 0.7122546962230558 1102 78-79 0.7918025592693603 1102 80-81 0.9654622515893081 1102 82-83 0.9565879487289699 1102 84-85 0.671367239619979 1102 86-87 0.788663261140151 1102 88-89 0.5430426862637283 1102 90-91 0.6550581377982532 1102 92-93 0.6025438902503311 1102 94-95 0.5032010656864081 1102 96-97 0.2479338688147834 1102 98-99 0.3002701515582018 1102 100-101 0.30795432200031314 1103 1 0.21569806929078084 1103 2 0.3037283940875497 1103 3 0.24235305401038687 1103 4 0.1371572245704229 1103 5 0.151203227481723 1103 6 0.16373296994272835 1103 7 0.22108375538714853 1103 8 0.2117328675361918 1103 9 0.12002731317898707 1103 10-11 0.20049318931833682 1103 12-13 0.23528459354422893 1103 14-15 0.35914039990208835 1103 16-17 0.3114444201976383 1103 18-19 0.24319551710552645 1103 20-21 0.2605716686817061 1103 22-23 0.320345045630944 1103 24-25 0.22330714537634577 1103 26-27 0.22129441843844688 1103 28-29 0.28822015881929985 1103 30-31 0.333611790099944 1103 32-33 0.29811816402285984 1103 34-35 0.3153300123907812 1103 36-37 0.2949494360848277 1103 38-39 0.07229372105678777 1103 40-41 0.37140687843061926 1103 42-43 0.47766843247314483 1103 44-45 0.5183826581992292 1103 46-47 0.45098972468317555 1103 48-49 0.5048011790180311 1103 50-51 0.5316764574188397 1103 52-53 0.5907588025000265 1103 54-55 0.48702045808323646 1103 56-57 0.46414706365498404 1103 58-59 0.3095405334351753 1103 60-61 0.3076445946554216 1103 62-63 0.3944921669662875 1103 64-65 0.36303484276555764 1103 66-67 0.24960315935021882 1103 68-69 0.25338859288457627 1103 70-71 0.21591031222817847 1103 72-73 0.5543330999259766 1103 74-75 0.5151147416910042 1103 76-77 0.5505385893815848 1103 78-79 0.24265954772549492 1103 80-81 0.46842628571042155 1103 82-83 0.3015536586290892 1103 84-85 0.4921288565354871 1103 86-87 0.5646961887894477 1103 88-89 0.37114683804142246 1103 90-91 0.4184215952542374 1103 92-93 0.48673576694679355 1103 94-95 0.3339397286541548 1103 96-97 0.4350667200682743 1103 98-99 0.46393663985063327 1103 100-101 0.37459074132528514 1104 1 0.25277784623602173 1104 2 0.273715168827394 1104 3 0.16166041779599993 1104 4 0.20027266066958305 1104 5 0.10192981417851854 1104 6 0.1434645230234679 1104 7 0.15360532112239866 1104 8 0.21424932330916135 1104 9 0.2728958034392903 1104 10-11 0.18067197557299153 1104 12-13 0.23250925085124408 1104 14-15 0.2918755495758276 1104 16-17 0.20581204675838904 1104 18-19 0.24916052857842175 1104 20-21 0.24743255553215704 1104 22-23 0.10322729622599525 1104 24-25 0.18477178720188192 1104 26-27 0.08000324779334989 1104 28-29 0.18693580145725264 1104 30-31 0.29078450950058254 1104 32-33 0.2322582411984584 1104 34-35 0.21054359187031224 1104 36-37 0.12700691379674112 1104 38-39 0.16949982911152972 1104 40-41 0.213141715085726 1104 42-43 0.19371014688778132 1104 44-45 0.3673051517538113 1104 46-47 0.3613935365545231 1104 48-49 0.42435262599348533 1104 50-51 0.34695372860979745 1104 52-53 0.36035506197195843 1104 54-55 0.16662314736809947 1104 56-57 0.4288110056029595 1104 58-59 0.3802565256551915 1104 60-61 0.2272964572946421 1104 62-63 0.3584914571006692 1104 64-65 0.32087230315889315 1104 66-67 0.39890582339739566 1104 68-69 0.45129011954154663 1104 70-71 0.18049372545111808 1104 72-73 0.3149672168165871 1104 74-75 0.37923647472624467 1104 76-77 0.26236543211157226 1104 78-79 0.36895507476949874 1104 80-81 0.36908826215108803 1104 82-83 0.4323604886778085 1104 84-85 0.5958245275145586 1104 86-87 0.5192862815267887 1104 88-89 0.5436910017599779 1104 90-91 0.630460327519863 1104 92-93 0.6413543969953324 1104 94-95 0.5497309389839522 1104 96-97 0.8824048577768515 1104 98-99 0.6798939016599519 1104 100-101 0.6312290103946729 1105 1 0.06332676051174602 1105 2 0.17930111734975895 1105 3 0.06070451417246403 1105 4 0.08496967355072371 1105 5 0.08494900261641192 1105 6 0.05228319416887928 1105 7 0.049769765829033474 1105 8 0.03307985706999261 1105 9 0.04897454448102678 1105 10-11 0.09163999057859229 1105 12-13 0.08893109085732931 1105 14-15 0.16037216741552385 1105 16-17 0.1665321480277342 1105 18-19 0.22350860003273 1105 20-21 0.08099309573960056 1105 22-23 0.0852003320533683 1105 24-25 0.1050466956735221 1105 26-27 0.3599010899914248 1105 28-29 0.20624613501068723 1105 30-31 0.19043088770691696 1105 32-33 0.17683960601758741 1105 34-35 0.028225534467757996 1105 36-37 0.1396980110069208 1105 38-39 0.11856663432937609 1105 40-41 0.038212116047809275 1105 42-43 0.1830377022010481 1105 44-45 0.10477841822340395 1105 46-47 0.10401556192729089 1105 48-49 0.0319663247910853 1105 50-51 0.12051699458897502 1105 52-53 0.13290211618389947 1105 54-55 0.1469656954920069 1105 56-57 0.1461507874417194 1105 58-59 0.26062579842497513 1105 60-61 0.23593043697111682 1105 62-63 0.15622800042409324 1105 64-65 -0.0031463479430442476 1105 66-67 0.1119147555416724 1105 68-69 -0.034588908079371095 1105 70-71 -0.06848304400829619 1105 72-73 0.03380173084382676 1105 74-75 0.1279191664666932 1105 76-77 0.010716997671181616 1105 78-79 0.11016108482962395 1105 80-81 0.047638037870182615 1105 82-83 0.17355734767900444 1105 84-85 0.113956950977947 1105 86-87 0.13103039517476844 1105 88-89 0.41978619693873753 1105 90-91 0.29079523139998287 1105 92-93 0.24065546932174797 1105 94-95 0.3203050071218492 1105 96-97 0.27978950290411575 1105 98-99 0.24443747527107007 1105 100-101 0.10016685142709747 1106 1 0.06734777811398729 1106 2 0.2063465679146077 1106 3 0.15560038946802734 1106 4 0.14507195921496674 1106 5 0.16432618100331098 1106 6 0.11177999774186986 1106 7 0.18126043926803703 1106 8 0.11810183797900464 1106 9 0.1483415643601731 1106 10-11 0.137108945828615 1106 12-13 0.19005308052367553 1106 14-15 0.14079076799350076 1106 16-17 0.21812896035806517 1106 18-19 0.09477666203465418 1106 20-21 0.025139518368547442 1106 22-23 0.001784008382294644 1106 24-25 0.11067380838041174 1106 26-27 -0.023323535632535197 1106 28-29 0.08032827880611393 1106 30-31 0.047863850313099476 1106 32-33 0.033513395316127514 1106 34-35 -0.031220476566481636 1106 36-37 0.053919659137235953 1106 38-39 0.0896597247926394 1106 40-41 -0.05285023617216922 1106 42-43 0.025497462250086755 1106 44-45 0.07167350535887351 1106 46-47 -0.08194511764184398 1106 48-49 -0.011975438936033811 1106 50-51 -0.06474239765203293 1106 52-53 -0.11760379501672702 1106 54-55 -0.15637916833144772 1106 56-57 -0.14276470428260524 1106 58-59 -0.2292982754869186 1106 60-61 -0.12601544270310683 1106 62-63 -0.020303569765943053 1106 64-65 0.07386486144481808 1106 66-67 -0.19462802403622703 1106 68-69 -0.23014552424540824 1106 70-71 -0.2031478132525386 1106 72-73 -0.0864786789994163 1106 74-75 0.035903665985042466 1106 76-77 -0.14903464432899938 1106 78-79 -0.4716316290682663 1106 80-81 -0.5034568040599368 1106 82-83 -0.21619779725488186 1106 84-85 -0.14003464198115978 1106 86-87 -0.09688737345646103 1106 88-89 -0.09585107427590245 1106 90-91 -0.006337442214171318 1106 92-93 -0.09213952148305182 1106 94-95 -0.15375349187106124 1106 96-97 -0.026957672855580483 1106 98-99 0.020452187592638893 1106 100-101 -0.15802677323355496 1107 1 0.03148500943491683 1107 2 0.15631215075413252 1107 3 0.011951120887353284 1107 4 0.08220147182074555 1107 5 0.006334009048707401 1107 6 0.02141181721577823 1107 7 0.03601862263672473 1107 8 -9.699919906012155E-4 1107 9 0.020838360984058113 1107 10-11 0.028670400358166148 1107 12-13 0.014077657962289436 1107 14-15 0.09513883175490179 1107 16-17 0.10877492716540615 1107 18-19 0.07011569511798399 1107 20-21 0.06962476971877862 1107 22-23 0.09826339963150588 1107 24-25 0.1539687595097945 1107 26-27 0.1667786662785531 1107 28-29 0.22122636115365424 1107 30-31 0.2766491038499339 1107 32-33 0.15468725226843816 1107 34-35 0.06532355072234708 1107 36-37 0.2051560967206001 1107 38-39 0.2638821899709498 1107 40-41 0.3189209574954148 1107 42-43 0.2863280222395943 1107 44-45 0.26963340302576455 1107 46-47 0.27539028567742463 1107 48-49 0.27189992458702505 1107 50-51 0.284936477558432 1107 52-53 0.1947956539588418 1107 54-55 0.30914113429466283 1107 56-57 0.5223312220725447 1107 58-59 0.29065615566782554 1107 60-61 0.32071711365663447 1107 62-63 0.1679794643669581 1107 64-65 0.24245620686279779 1107 66-67 0.2547254843712672 1107 68-69 0.2793279850367263 1107 70-71 0.10924551322943543 1107 72-73 0.249952869335484 1107 74-75 0.2029318513216687 1107 76-77 -0.11078593989237362 1107 78-79 0.1635008135880227 1107 80-81 0.19889300898535112 1107 82-83 0.3501186044681681 1107 84-85 0.366384355241852 1107 86-87 0.4776403417601216 1107 88-89 0.3080276128865371 1107 90-91 0.2873794799208298 1107 92-93 0.18631918448419427 1107 94-95 0.34057156880722417 1107 96-97 0.6948443519405174 1107 98-99 0.8234153870328704 1107 100-101 0.4941918872523061 1108 1 -0.02420823184333898 1108 2 0.12820876563864658 1108 3 -0.09926465320622668 1108 4 0.025829782828054704 1108 5 0.027818713009004625 1108 6 -0.04286509471901212 1108 7 0.06716335534201079 1108 8 0.10592438420944461 1108 9 0.012101877304701247 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5 64.0 6 150.0 7 376.0 8 900.0 9 1707.0 10 3029.0 11 4804.0 12 7004.0 13 9472.0 14 12018.0 15 15980.0 16 20228.0 17 25178.0 18 22787.0 19 16046.0 20 15148.0 21 15599.0 22 16637.0 23 19110.0 24 22174.0 25 26130.0 26 31941.0 27 38978.0 28 47553.0 29 58575.0 30 71483.0 31 86647.0 32 107248.0 33 133992.0 34 174499.0 35 232360.0 36 316413.0 37 377969.0 38 189660.0 39 7726.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.34619152411357 19.511200902421454 15.261235419478517 21.88137215398646 2 15.51943804073153 21.39501748608885 39.24665411544153 23.838890357738094 3 20.432897302292734 24.782626254277975 30.061206511163117 24.723269932266174 4 13.003377134657141 17.330777003929345 37.480888573339044 32.184957288074465 5 13.212126107232566 38.11124661367509 33.99942991874699 14.677197360345353 6 30.747616243633846 37.01145085841525 17.178118933782617 15.06281396416829 7 27.879298427509937 33.24965627606103 20.70030465586284 18.170740640566187 8 26.118507986089263 33.97466540319255 19.463856219401215 20.442970391316965 9 25.70525525927408 17.577839040692254 21.03404411775064 35.682861582283024 10-11 25.095016862147368 26.680697325628678 26.892824172552686 21.331461639671264 12-13 26.78987162980097 24.155202190811327 27.371125016552234 21.68380116283547 14-15 22.92388335345436 25.78433264635406 26.982226348167483 24.3095576520241 16-17 21.405928956674273 28.77715121847223 27.572238306876418 22.244681517977078 18-19 21.21123044309306 27.70664633721147 29.99608139890068 21.086041820794787 20-21 22.639265686282567 25.986901342148727 29.929083176125175 21.444749795443528 22-23 21.848557389796827 26.079445457670225 30.09240650409558 21.97959064843737 24-25 22.300235911518154 26.218179649626315 30.186367281927414 21.295217156928118 26-27 21.959379109450825 25.933072050228716 30.833691574335965 21.273857265984493 28-29 21.247011496654682 27.23185996371652 30.235823817353285 21.285304722275505 30-31 21.755414324390127 26.174073085367223 30.055590108120967 22.014922482121687 32-33 21.699532152263938 26.358639123303213 30.178644298132127 21.763184426300715 34-35 22.57971813446394 26.17112533498122 29.928667254884374 21.320489275670468 36-37 22.267989922966574 25.962213472514726 30.416065026448692 21.353731578070008 38-39 21.761331992174696 26.651865083949204 30.240121868868876 21.346681055007227 40-41 22.010776098370577 26.66482126745117 29.75269537459843 21.571707259579817 42-43 21.693555241358553 26.790472912404304 29.15482362575725 22.361148220479894 44-45 22.176711183740938 26.37371332506856 29.148681392989968 22.300894098200533 46-47 22.83931138953298 27.079030926615143 28.69551497871453 21.386142705137342 48-49 22.624056709760442 26.968339539172153 28.566400753575643 21.84120299749176 50-51 21.95996155151923 27.433634344053797 28.87470105930542 21.731703045121556 52-53 22.61065742930758 27.71019898920602 27.85409837374208 21.825045207744317 54-55 22.08072850572602 27.175793610079175 28.209546353233524 22.533931530961283 56-57 22.70108505507459 27.203065673891757 27.934873214566647 22.160976056467007 58-59 23.273693918413805 26.885586614424657 27.823871982028347 22.01684748513319 60-61 22.50921200982176 27.24463350828365 28.763940105853003 21.48221437604159 62-63 22.124802605536484 27.30662151304999 28.67681965647376 21.891756224939765 64-65 21.94767875267867 27.967949180004936 28.105818326876197 21.978553740440198 66-67 22.27779074315729 27.305793615054075 27.682107885836 22.734307755952635 68-69 22.156745443847722 27.35323134514435 28.046438920488566 22.44358429051936 70-71 22.793781339133684 27.168534218412926 27.744413545549573 22.29327089690382 72-73 22.539370679579466 26.886709833501364 28.46447188559928 22.109447601319893 74-75 22.01916482211926 28.23940836107688 27.5869938804151 22.154432936388766 76-77 22.367781570350502 27.38571685494137 27.61005465343038 22.63644692127774 78-79 22.42961534686686 27.52192204940462 27.731926665561545 22.316535938166975 80-81 22.828434089896827 27.408809363984687 27.479575039673627 22.283181506444866 82-83 22.62017544910025 27.34835096488083 27.912316303317557 22.11915728270136 84-85 22.29145075956256 27.80718366664616 27.640460729129344 22.26090484466193 86-87 22.268725259554568 27.876583036330594 27.388019402613622 22.466672301501216 88-89 22.211544557012548 27.804115844710797 26.959815925712878 23.024523672563774 90-91 22.384182340377972 27.30172512571215 27.363829839189886 22.950262694719996 92-93 22.729235347248945 27.58612107723867 27.467341480657286 22.2173020948551 94-95 22.384017208740264 27.40497052167398 27.59268046274081 22.618331806844942 96-97 21.75727207542758 27.510226007562856 28.052646975820554 22.679854941189014 98-99 22.016876803000738 27.79378097252102 27.099874413595543 23.0894678108827 100-101 21.826924905562297 27.451817343197256 27.257600506391288 23.463657244849156 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 530.0 1 393.0 2 437.0 3 584.5 4 452.5 5 376.0 6 595.5 7 1219.5 8 1858.0 9 2218.0 10 2227.0 11 2011.5 12 2024.0 13 2239.0 14 2535.0 15 2819.5 16 3011.5 17 3264.0 18 3589.5 19 3997.5 20 4480.0 21 5145.5 22 6128.5 23 7367.5 24 8775.0 25 10606.0 26 13540.5 27 17210.0 28 20278.5 29 23526.0 30 27611.0 31 31748.5 32 36372.5 33 40388.5 34 44004.0 35 47917.5 36 52665.5 37 56909.0 38 58289.0 39 59356.0 40 60825.0 41 62172.0 42 63098.0 43 64674.0 44 67843.0 45 71128.5 46 73398.5 47 83589.5 48 106389.5 49 105356.5 50 83106.5 51 72552.5 52 68695.0 53 67018.0 54 65085.0 55 60515.0 56 54507.5 57 48815.5 58 45440.0 59 40996.5 60 33946.5 61 28205.0 62 23330.0 63 17907.5 64 12650.0 65 9161.5 66 6953.5 67 5436.0 68 4254.5 69 3334.0 70 2604.0 71 1943.0 72 1556.0 73 1340.5 74 1033.5 75 739.0 76 560.0 77 357.5 78 234.0 79 172.5 80 106.0 81 64.0 82 35.0 83 25.0 84 16.0 85 7.5 86 2.0 87 0.5 88 1.0 89 2.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011269605591978295 2 0.010095688342813889 3 0.005165235896323385 4 2.817401397994574E-4 5 0.004742625686624199 6 0.0 7 2.817401397994574E-4 8 0.002066094358529354 9 1.408700698997287E-4 10-11 0.0 12-13 0.0 14-15 4.2261020969918604E-4 16-17 0.0042495804419751485 18-19 0.0016669624938134562 20-21 6.808720045153553E-4 22-23 4.6956689966576234E-4 24-25 4.6956689966576234E-4 26-27 1.643484148830168E-4 28-29 1.1739172491644058E-4 30-31 7.747853844485078E-4 32-33 0.003897405267225827 34-35 0.0011739172491644057 36-37 0.0012678306290975583 38-39 0.005634802795989147 40-41 7.043503494986434E-5 42-43 0.001455657388963863 44-45 0.004789582376590775 46-47 0.0 48-49 0.0019487026336129135 50-51 8.921771093649483E-4 52-53 4.2261020969918604E-4 54-55 1.1739172491644058E-4 56-57 0.011128735522078565 58-59 0.0021600077384625067 60-61 0.0030521848478274544 62-63 1.408700698997287E-4 64-65 0.003122619882777319 66-67 8.921771093649483E-4 68-69 0.0013617440090307107 70-71 0.004507842236791318 72-73 0.0025826179481616925 74-75 0.0069261117700699934 76-77 9.860904892981007E-4 78-79 0.006808720045153553 80-81 0.0027469663630447093 82-83 0.0027469663630447093 84-85 0.0019252242886296255 86-87 5.634802795989148E-4 88-89 0.0022069644284290826 90-91 0.007231330254852739 92-93 0.002230442773412371 94-95 3.052184847827455E-4 96-97 1.408700698997287E-4 98-99 5.869586245822029E-4 100-101 0.002042616013546066 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2129622.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.88705729342264 #Duplication Level Percentage of deduplicated Percentage of total 1 79.91245339344289 47.05809221437639 2 12.274045626205456 14.455648560248887 3 3.4882824822934566 6.162440711713722 4 1.4373109289973116 3.3855604409730886 5 0.7292161132846283 2.147069552113944 6 0.43897324910709073 1.5509905722269486 7 0.3042923002355401 1.2543214682542332 8 0.22431715319697096 1.0567501641765995 9 0.1559419962908582 0.8264668743027422 >10 0.9257412217623755 10.091129406490529 >50 0.0704897717833845 2.8538494051667427 >100 0.03462963322982468 3.711117246635524 >500 0.0022328082363996995 0.9067666111885173 >1k 0.0016746061772997542 2.362664695771033 >5k 3.1897260519995324E-4 1.4362225169964034 >10k+ 7.974315129998831E-5 0.7409095593646968 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15778 0.7408826542926397 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9277 0.4356172128199277 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8392 0.39406054219950765 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7908 0.3713335042556848 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5008 0.23515910335261378 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4821 0.226378202328864 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4632 0.2175033879251811 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4005 0.18806154331613778 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3883 0.1823328271402155 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3642 0.17101626485827062 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2966 0.1392735424408651 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2892 0.13579874738333847 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2745 0.12889611395825176 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2603 0.12222826398299792 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2460 0.11551345731777751 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2305 0.10823517037295821 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 4.6956689966576225E-5 16-17 0.0 0.0 0.0 0.0 4.6956689966576225E-5 18-19 0.0 0.0 0.0 4.6956689966576225E-5 7.043503494986433E-5 20-21 0.0 0.0 0.0 7.043503494986433E-5 9.391337993315245E-5 22-23 0.0 0.0 0.0 9.391337993315245E-5 1.1739172491644057E-4 24-25 0.0 0.0 0.0 2.1130510484959302E-4 1.408700698997287E-4 26-27 0.0 0.0 0.0 2.5826179481616923E-4 1.408700698997287E-4 28-29 0.0 0.0 0.0 4.695668996657623E-4 1.408700698997287E-4 30-31 0.0 0.0 0.0 0.0011034822142145413 1.408700698997287E-4 32-33 0.0 0.0 0.0 0.0019017459436463371 1.408700698997287E-4 34-35 0.0 0.0 0.0 0.0030756631928107432 1.408700698997287E-4 36-37 0.0 0.0 0.0 0.00410871037207542 1.408700698997287E-4 38-39 0.0 0.0 0.0 0.005470454381106131 1.408700698997287E-4 40-41 0.0 0.0 0.0 0.007301765289802603 1.408700698997287E-4 42-43 0.0 0.0 0.0 0.010424385172579922 1.408700698997287E-4 44-45 0.0 0.0 0.0 0.014861792374421375 1.408700698997287E-4 46-47 0.0 0.0 0.0 0.025074872442151706 1.408700698997287E-4 48-49 0.0 0.0 0.0 0.04526624912777948 1.408700698997287E-4 50-51 0.0 0.0 0.0 0.062100222480797065 1.408700698997287E-4 52-53 0.0 0.0 0.0 0.07841767224418231 1.408700698997287E-4 54-55 0.0 0.0 0.0 0.10198993060740356 1.878267598663049E-4 56-57 0.0 0.0 0.0 0.12056130148918447 1.878267598663049E-4 58-59 0.0 0.0 0.0 0.14519008537665368 1.878267598663049E-4 60-61 0.0 0.0 0.0 0.18618327571747476 1.878267598663049E-4 62-63 0.0 0.0 0.0 0.21520251011681885 1.878267598663049E-4 64-65 0.0 0.0 0.0 0.24882350013288745 1.878267598663049E-4 66-67 0.0 0.0 0.0 0.31101763599361765 1.878267598663049E-4 68-69 0.0 4.6956689966576225E-5 0.0 0.38605442656020644 1.878267598663049E-4 70-71 0.0 4.6956689966576225E-5 0.0 0.45066683195421536 1.878267598663049E-4 72-73 0.0 4.6956689966576225E-5 0.0 0.5190592508905336 1.878267598663049E-4 74-75 0.0 4.6956689966576225E-5 0.0 0.5762055425798569 1.878267598663049E-4 76-77 0.0 4.6956689966576225E-5 0.0 0.6241952797256978 1.878267598663049E-4 78-79 0.0 4.6956689966576225E-5 0.0 0.6913198680329184 1.878267598663049E-4 80-81 0.0 4.6956689966576225E-5 0.0 0.7888019564035307 2.3478344983288114E-4 82-83 0.0 4.6956689966576225E-5 0.0 0.900629313558932 2.817401397994574E-4 84-85 0.0 4.6956689966576225E-5 0.0 1.031591521875713 2.817401397994574E-4 86-87 0.0 4.6956689966576225E-5 0.0 1.240525313881994 2.817401397994574E-4 88-89 0.0 4.6956689966576225E-5 2.3478344983288113E-5 1.500313201122077 2.817401397994574E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1955 0.0 25.027344 2 GTACCTG 2070 0.0 25.015022 1 GAGTACT 10775 0.0 21.888247 12-13 GTACATG 9530 0.0 21.684084 1 TACATGG 9395 0.0 21.033953 2 GTACTTT 11300 0.0 20.598076 14-15 AGTACTT 11235 0.0 19.998484 12-13 ACATGGG 9470 0.0 19.962578 3 TATACCG 155 9.6296944E-4 18.386671 5 AGAGTAC 17480 0.0 18.166193 10-11 ACTTTTT 13190 0.0 17.807583 16-17 TATACGA 135 0.00939913 17.593836 2 CTAGACG 135 0.009404215 17.592184 4 CATGGGG 4435 0.0 17.457317 4 TACTTTT 12870 0.0 17.347157 14-15 GTATAGC 840 0.0 16.966282 1 TAGACTC 845 0.0 16.301397 5 TCTAGAC 915 0.0 16.092524 3 AGTACAT 7620 0.0 16.021473 2 ATACCGT 190 0.003757662 14.998595 6 >>END_MODULE