FastQCFastQC Report
Thu 26 May 2016
SRR936730_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936730_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203563
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT135190.6135063985009732No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT91760.4164165036352489No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89610.4066595781468467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72670.3297840815079941No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC54110.24555685496625237No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT51200.232350969770322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43750.1985420884267888No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT39750.1803896689134824No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT33680.15284337230203993No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC31800.1443117351307859No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC31180.14149811010622343No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG26730.12130354339767004No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC25270.11467791027531321No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA24750.11231809573858337No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA22320.10129050088424973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG19400.030.6223812
GTACCTG21150.029.4388711
GAGTACT89450.022.9459412-13
GTACATG102950.022.6219461
TACATGG101950.022.3761142
ACATGGG101150.021.0965333
GTACTTT96500.020.97226514-15
GTATTAG6800.019.5708351
CATGGGA52950.019.2975924
AGTACTT94500.019.20587312-13
GTGTAGC14050.018.6057261
ACCTGGG31850.018.5030333
AGAGTAC155450.018.37007310-11
ACTTTTT114200.018.22033116-17
CATGGGG45950.016.4453184
TACTTTT109550.016.30566414-15
GTATAGG6550.015.9639961
GTATTGA6750.015.490991
ATGGGAG26600.015.3655275
GTATCAA276650.015.3419661
Produced by FastQC (version 0.11.5)