##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936730_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2203563 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.17719075878475 31.0 31.0 34.0 28.0 34.0 2 31.216191685919576 31.0 31.0 34.0 28.0 34.0 3 31.248375471906183 31.0 31.0 34.0 27.0 34.0 4 34.88673752463624 37.0 35.0 37.0 32.0 37.0 5 34.81904896751307 37.0 35.0 37.0 32.0 37.0 6 34.93942265322117 37.0 35.0 37.0 32.0 37.0 7 34.785330394456615 37.0 35.0 37.0 32.0 37.0 8 34.765802929165176 37.0 35.0 37.0 32.0 37.0 9 36.23672751811498 39.0 35.0 39.0 32.0 39.0 10-11 36.09737865447913 39.0 35.0 39.0 31.0 39.0 12-13 36.00493995406531 39.0 35.0 39.0 30.0 39.0 14-15 37.00293683457201 39.0 36.0 41.0 31.0 41.0 16-17 36.94091932021004 39.0 36.0 41.0 31.0 41.0 18-19 36.927709350719724 39.0 36.0 41.0 31.0 41.0 20-21 36.83265919785366 39.0 36.0 41.0 30.0 41.0 22-23 36.71724361863038 39.0 36.0 41.0 30.0 41.0 24-25 36.55976434528988 39.0 36.0 41.0 30.0 41.0 26-27 36.344738952324036 39.0 35.5 40.0 29.5 41.0 28-29 36.453332625388974 39.0 36.0 41.0 30.0 41.0 30-31 36.37824151158827 39.0 35.5 40.5 30.0 41.0 32-33 36.19401215213724 39.0 35.0 40.0 30.0 41.0 34-35 35.992605158100766 38.5 35.0 40.0 29.0 41.0 36-37 35.73828000379386 38.0 35.0 40.0 27.5 41.0 38-39 35.54170132644268 38.0 35.0 40.0 27.0 41.0 40-41 35.4000076240162 38.0 34.5 40.0 27.0 41.0 42-43 35.11833040398663 38.0 34.0 40.0 25.5 41.0 44-45 34.82048936200145 38.0 34.0 40.0 24.5 41.0 46-47 34.44792592723694 38.0 33.0 40.0 23.5 41.0 48-49 34.15653443990483 38.0 33.0 40.0 23.0 41.0 50-51 33.44228007095781 37.5 32.0 39.5 22.5 40.5 52-53 33.63282216119984 37.5 33.0 39.5 22.0 40.5 54-55 33.996743002128824 38.0 33.0 40.0 22.0 41.0 56-57 33.902410096738784 38.0 33.0 40.0 22.0 41.0 58-59 33.65883367074143 38.0 33.0 40.0 20.5 41.0 60-61 33.31063395963719 37.0 32.5 40.0 19.5 41.0 62-63 33.011858521857555 37.0 32.0 40.0 18.0 41.0 64-65 32.713010474399866 36.0 32.0 39.0 18.0 41.0 66-67 32.319866507106894 36.0 31.0 39.0 14.5 41.0 68-69 31.9278704534429 35.0 31.0 39.0 12.5 40.0 70-71 31.51516884246105 35.0 31.0 38.0 9.5 40.0 72-73 31.095630576479998 35.0 30.5 37.5 7.5 40.0 74-75 30.673408248368666 35.0 30.0 37.0 7.0 39.0 76-77 30.26187383796152 34.5 30.0 36.5 3.5 39.0 78-79 29.797106549710627 34.0 29.0 36.0 2.0 38.5 80-81 29.360637748954762 34.0 29.0 35.5 2.0 37.0 82-83 28.939628683182647 34.0 29.0 35.0 2.0 37.0 84-85 28.53622927958039 34.0 28.0 35.0 2.0 36.5 86-87 28.21246703634069 34.0 27.0 35.0 2.0 36.0 88-89 27.821052767722094 34.0 26.5 35.0 2.0 36.0 90-91 27.43847078572294 33.5 26.0 35.0 2.0 35.0 92-93 27.056893767049093 33.0 25.5 35.0 2.0 35.0 94-95 26.615037101276435 33.0 25.0 35.0 2.0 35.0 96-97 26.021897490564147 33.0 24.0 35.0 2.0 35.0 98-99 25.36642905149524 32.5 20.5 35.0 2.0 35.0 100-101 23.32533174681187 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2892043757113889 1101 2 0.29701845771730007 1101 3 0.27819984273167364 1101 4 0.2055457919600414 1101 5 0.23612662270706863 1101 6 0.1949344609377448 1101 7 0.2628593767456451 1101 8 0.263382625940622 1101 9 0.35160471881707167 1101 10-11 0.24421344898139097 1101 12-13 0.4706158299625116 1101 14-15 0.4898533898628372 1101 16-17 0.5976177641662659 1101 18-19 0.6272488440356554 1101 20-21 0.5588295856727186 1101 22-23 0.5769175487777645 1101 24-25 0.6131103195561778 1101 26-27 0.5277225395724159 1101 28-29 0.7452332855320662 1101 30-31 0.5064116251085764 1101 32-33 0.5176756472056852 1101 34-35 0.42210235047707556 1101 36-37 0.5416341613125937 1101 38-39 0.3541095977298667 1101 40-41 0.39833266976526716 1101 42-43 0.1959264777819314 1101 44-45 0.35073021027800877 1101 46-47 0.5831915820843534 1101 48-49 0.4887395577935578 1101 50-51 0.6891405566406874 1101 52-53 0.7034514789299493 1101 54-55 0.6539731517238394 1101 56-57 0.5931055794600795 1101 58-59 0.7035872293748042 1101 60-61 0.6504438284513867 1101 62-63 0.6881348246683316 1101 64-65 0.6860154877299252 1101 66-67 0.4524930452153697 1101 68-69 0.6324967753086455 1101 70-71 0.45359027904512317 1101 72-73 0.5920915484831681 1101 74-75 0.4002312154885743 1101 76-77 0.4104194009565383 1101 78-79 0.3129778813502213 1101 80-81 0.19791461577664293 1101 82-83 0.41053622523992317 1101 84-85 0.46605424863215816 1101 86-87 0.6270446042460414 1101 88-89 0.3446703581797941 1101 90-91 0.40705574989694426 1101 92-93 0.3675065599306677 1101 94-95 0.268118566021581 1101 96-97 0.3092661128786425 1101 98-99 0.047186297972608315 1101 100-101 0.37898375783912996 1102 1 0.22375355237861427 1102 2 0.29492337978156513 1102 3 0.186708555853496 1102 4 0.37049108619666526 1102 5 0.44607382824886344 1102 6 0.31475738202120596 1102 7 0.12510783136045234 1102 8 0.2554439506034427 1102 9 0.4401708703382283 1102 10-11 0.4912521237367926 1102 12-13 0.5650432032748043 1102 14-15 0.5083818423011124 1102 16-17 0.3526243741679522 1102 18-19 0.30560715438028296 1102 20-21 0.06145861417186893 1102 22-23 -0.03392463353917918 1102 24-25 0.13905607339581394 1102 26-27 0.20271236704846274 1102 28-29 0.12067410916256449 1102 30-31 0.1422643563927508 1102 32-33 0.16975821695805848 1102 34-35 0.1466082430121034 1102 36-37 0.13013751525105732 1102 38-39 0.09760842237129452 1102 40-41 0.029889755505692506 1102 42-43 0.36934037682052434 1102 44-45 0.13339787212380827 1102 46-47 0.36939550072196425 1102 48-49 0.4602423741025845 1102 50-51 0.4039246304892785 1102 52-53 0.25179090208880694 1102 54-55 0.30944328317440295 1102 56-57 0.4729650771410121 1102 58-59 0.4041870061781694 1102 60-61 0.07716888831441793 1102 62-63 0.34825719611038153 1102 64-65 0.3748462788733846 1102 66-67 0.23778562890641552 1102 68-69 0.334076759066825 1102 70-71 0.4129437308759556 1102 72-73 0.32134276516494253 1102 74-75 0.17646837064514642 1102 76-77 0.28658641788656425 1102 78-79 0.25010911666251445 1102 80-81 0.40638393924305305 1102 82-83 0.43720398016987616 1102 84-85 0.14927372498587488 1102 86-87 0.18632064565069584 1102 88-89 0.11682686823579047 1102 90-91 0.060668304896164926 1102 92-93 0.1559884737135242 1102 94-95 0.3171381537340636 1102 96-97 0.04142013498681507 1102 98-99 0.022567335566627378 1102 100-101 0.2593759227541419 1103 1 0.17975510489407398 1103 2 0.32436243151919086 1103 3 0.27270722602676045 1103 4 0.18603943893892705 1103 5 0.2695306177577379 1103 6 -0.011981743110780485 1103 7 0.20252452262741372 1103 8 -0.06336549770431787 1103 9 0.38557662098426704 1103 10-11 0.37339120993973296 1103 12-13 0.36302959603101925 1103 14-15 0.46180405531930546 1103 16-17 0.607226953607011 1103 18-19 0.27421667234879266 1103 20-21 0.22778220412658357 1103 22-23 0.17800451294735353 1103 24-25 0.2611513918050221 1103 26-27 0.34124043769289614 1103 28-29 0.3623395022554661 1103 30-31 0.09911567715097647 1103 32-33 0.15316873632890804 1103 34-35 0.214005351941573 1103 36-37 0.2881003215870379 1103 38-39 0.2263980925347724 1103 40-41 0.006343326316148534 1103 42-43 0.2611820209071851 1103 44-45 0.37684137332163203 1103 46-47 0.3687566396138493 1103 48-49 0.5280912491013581 1103 50-51 -0.19113582817317365 1103 52-53 0.10143676561247617 1103 54-55 0.4180456890423656 1103 56-57 0.4990762432882079 1103 58-59 0.4015923174205156 1103 60-61 0.5998355144922272 1103 62-63 0.4244314269755378 1103 64-65 0.4689965678792092 1103 66-67 0.30405494055762716 1103 68-69 0.21212514292245999 1103 70-71 0.28833810315675024 1103 72-73 0.3308949832658179 1103 74-75 0.1535740775997212 1103 76-77 0.004188153199269351 1103 78-79 0.20588366606216368 1103 80-81 0.3725706801702735 1103 82-83 0.2879296019352289 1103 84-85 0.21001282257215692 1103 86-87 0.041386872316291345 1103 88-89 0.12774541941827167 1103 90-91 0.3421427100459482 1103 92-93 0.36458334599855036 1103 94-95 0.18690382777650427 1103 96-97 0.23202094486357083 1103 98-99 0.2397723962094851 1103 100-101 0.19679814971112464 1104 1 0.14164906513819275 1104 2 0.22943440497596868 1104 3 0.29805434233585615 1104 4 0.266498586637276 1104 5 0.2591148929244369 1104 6 0.17793607768470565 1104 7 0.4052130242620251 1104 8 0.30564754703968333 1104 9 0.4500213723352857 1104 10-11 0.48996376778858775 1104 12-13 0.44278893048974766 1104 14-15 0.5296368400295322 1104 16-17 0.26483239671663483 1104 18-19 0.4459317314487308 1104 20-21 0.24524317372395643 1104 22-23 0.4982476458850371 1104 24-25 0.5251378035714254 1104 26-27 0.37346595971283136 1104 28-29 0.41626113906994533 1104 30-31 0.35022956587702936 1104 32-33 0.2418001824848588 1104 34-35 0.38448480846522415 1104 36-37 0.3170135709096584 1104 38-39 0.3812672923730176 1104 40-41 0.18783573352465766 1104 42-43 0.35851849131150715 1104 44-45 0.14458882585902444 1104 46-47 0.4108915734097991 1104 48-49 0.35138915128449355 1104 50-51 0.16950736080256945 1104 52-53 0.371847764305258 1104 54-55 0.5488024643638951 1104 56-57 0.47738095647904544 1104 58-59 0.5172986369692154 1104 60-61 0.45687133678271863 1104 62-63 0.35516606196812717 1104 64-65 0.1250630345708501 1104 66-67 0.29947634702657666 1104 68-69 0.369284633108677 1104 70-71 0.16597188323787293 1104 72-73 0.015037026475393844 1104 74-75 -0.10176154296041773 1104 76-77 0.06834286127315536 1104 78-79 0.21203775016626736 1104 80-81 0.38716143846246354 1104 82-83 0.11250698991957009 1104 84-85 0.260284948245765 1104 86-87 0.138485462522155 1104 88-89 0.119453477073872 1104 90-91 0.08886413635342905 1104 92-93 -0.09974663001968054 1104 94-95 0.04389328288595351 1104 96-97 0.09351389523048681 1104 98-99 0.06312204747860051 1104 100-101 0.26094995983619285 1105 1 0.22804609685713828 1105 2 0.21986597459666157 1105 3 0.19970690975688044 1105 4 0.25379385044352887 1105 5 0.3111362167209464 1105 6 0.3303748160044009 1105 7 0.3141656542818225 1105 8 -0.046391470628719844 1105 9 0.26973455735286933 1105 10-11 0.2573104644633517 1105 12-13 0.08571704511386713 1105 14-15 0.1578840676544857 1105 16-17 0.12058763014839968 1105 18-19 0.040162657923993095 1105 20-21 -0.2032944679778197 1105 22-23 -0.334571571000275 1105 24-25 -0.2853217880486554 1105 26-27 -0.14250560811166935 1105 28-29 -0.13368534366981066 1105 30-31 -0.10106285752660682 1105 32-33 -0.2040547264252197 1105 34-35 -0.274614767820168 1105 36-37 -0.30728537643900466 1105 38-39 -0.38328139325379595 1105 40-41 -0.38710602414704454 1105 42-43 -0.2673913356236568 1105 44-45 -0.1807952786098852 1105 46-47 -0.2763603605170033 1105 48-49 0.09011014573371057 1105 50-51 0.07715892154951831 1105 52-53 0.06920715499265384 1105 54-55 0.13145027186529745 1105 56-57 0.1780800654853607 1105 58-59 0.028633606092064667 1105 60-61 0.22875962813108686 1105 62-63 0.17707221474397983 1105 64-65 -0.11278549784311309 1105 66-67 -0.294468059364128 1105 68-69 -0.0615180855815467 1105 70-71 0.021917794948134173 1105 72-73 0.005306234714840485 1105 74-75 -0.04860234905032357 1105 76-77 0.11634083988233712 1105 78-79 0.14233495359619752 1105 80-81 0.28467806332959356 1105 82-83 0.32878742974633113 1105 84-85 0.04377864346345817 1105 86-87 0.1867766634400887 1105 88-89 0.1885269347915539 1105 90-91 0.15715762272981593 1105 92-93 0.044890384219865354 1105 94-95 -0.04687443678950132 1105 96-97 -0.076009482797744 1105 98-99 -0.2099910753423373 1105 100-101 0.17871668490156978 1106 1 0.1136022213244452 1106 2 0.17214916298980398 1106 3 0.12276523379578208 1106 4 0.2170963963897634 1106 5 0.38774878384922573 1106 6 0.3206087181171853 1106 7 0.5520107885648429 1106 8 0.5285368531058836 1106 9 0.47124276886631833 1106 10-11 0.43488336785816273 1106 12-13 0.47623742075439424 1106 14-15 0.3268070317111764 1106 16-17 0.35717669328305135 1106 18-19 0.32242392336895165 1106 20-21 0.4524375163731378 1106 22-23 0.3490332853892042 1106 24-25 0.2117445126622286 1106 26-27 0.283955436648462 1106 28-29 0.21300798908137608 1106 30-31 0.24663830900709627 1106 32-33 0.23009522680494143 1106 34-35 0.22091381734922777 1106 36-37 0.2728126020720083 1106 38-39 0.2676206295582375 1106 40-41 0.26438277159908097 1106 42-43 0.3325117178447883 1106 44-45 0.1874396329904897 1106 46-47 0.2704136121673031 1106 48-49 0.22734670982426763 1106 50-51 0.42840553288620953 1106 52-53 0.26590938525902175 1106 54-55 0.5444967413709705 1106 56-57 0.48088764497754966 1106 58-59 0.39146253300543066 1106 60-61 0.4640904701601727 1106 62-63 0.45350445007763796 1106 64-65 0.30469385264488835 1106 66-67 0.42916784240730976 1106 68-69 0.34678971287551263 1106 70-71 0.34572632174453943 1106 72-73 0.4439476139156895 1106 74-75 0.3600993877181793 1106 76-77 0.09639353698710451 1106 78-79 0.33902792250847824 1106 80-81 0.2828932680915166 1106 82-83 0.18886159996208107 1106 84-85 0.3672744341780252 1106 86-87 0.511629573291394 1106 88-89 0.5060845437216166 1106 90-91 0.6516869739455053 1106 92-93 0.538957494637696 1106 94-95 0.5233922835930187 1106 96-97 0.5363479419636761 1106 98-99 0.5313894154829661 1106 100-101 0.7420405319622141 1107 1 0.4112474781953246 1107 2 0.4003005033207039 1107 3 0.5605669125213311 1107 4 0.48495770621608614 1107 5 0.4252207955968501 1107 6 0.3575410843881812 1107 7 0.5725676615388764 1107 8 0.5609797070936509 1107 9 0.6224364749500708 1107 10-11 0.7495358174296953 1107 12-13 0.6497601757134461 1107 14-15 0.7739373010635333 1107 16-17 0.7881222837161985 1107 18-19 0.9275732105849244 1107 20-21 0.9382489010001649 1107 22-23 0.9013969031783589 1107 24-25 1.0536468539188135 1107 26-27 0.8852803517328454 1107 28-29 0.6955848081753828 1107 30-31 0.6498234851277616 1107 32-33 0.7792976833402392 1107 34-35 0.7014676549322942 1107 36-37 0.897459407139273 1107 38-39 0.8126058770436657 1107 40-41 0.8071626752529824 1107 42-43 0.93704170813227 1107 44-45 0.8132338966895318 1107 46-47 0.749792430062584 1107 48-49 0.8034341496630688 1107 50-51 0.6106670718735288 1107 52-53 0.6193046868706773 1107 54-55 0.7152652281330276 1107 56-57 0.8305897650842198 1107 58-59 0.8710068091069445 1107 60-61 0.8114725924423283 1107 62-63 0.8524536756303362 1107 64-65 0.7964481359086051 1107 66-67 0.7102862039135545 1107 68-69 0.7769399831024799 1107 70-71 0.7951588579753839 1107 72-73 0.9370829816959372 1107 74-75 0.6420304328958935 1107 76-77 0.6321205664087124 1107 78-79 0.4947205116345046 1107 80-81 0.7258981985806976 1107 82-83 0.7799651299453885 1107 84-85 0.8617619473659914 1107 86-87 0.9195463396372112 1107 88-89 0.829805315681071 1107 90-91 0.6810977442917157 1107 92-93 0.6653180461674992 1107 94-95 0.7558621657897149 1107 96-97 0.8941832378279386 1107 98-99 0.9181126484609834 1107 100-101 1.1540267800977126 1108 1 0.0971111099110864 1108 2 0.15360680052487652 1108 3 -0.002295089709480891 1108 4 -0.02158772001570952 1108 5 0.37980572722321426 1108 6 0.3043493295632871 1108 7 0.3780383031356749 1108 8 0.022505006325069132 1108 9 0.21551946611032946 1108 10-11 0.05497071730127345 1108 12-13 0.2734023419434308 1108 14-15 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50-51 -2.3458502218388197 2316 52-53 -2.0441501624868224 2316 54-55 -1.9507521663110694 2316 56-57 -1.779443848306066 2316 58-59 -1.9332937179508178 2316 60-61 -2.0933620019919843 2316 62-63 -2.095351645577871 2316 64-65 -1.9550293681326423 2316 66-67 -2.668123263206674 2316 68-69 -2.2116494673207328 2316 70-71 -1.9369917661202045 2316 72-73 -1.8528985083724692 2316 74-75 -2.068469042769511 2316 76-77 -2.595222795722048 2316 78-79 -2.586037102059368 2316 80-81 -2.691697053436524 2316 82-83 -2.6114344463004784 2316 84-85 -2.627880710852825 2316 86-87 -2.7232187884686105 2316 88-89 -2.881891090174836 2316 90-91 -2.8507183596261285 2316 92-93 -3.1310186212732525 2316 94-95 -3.2041967503064086 2316 96-97 -3.255319570936308 2316 98-99 -3.6247700798641027 2316 100-101 -4.327805196319712 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5829.0 3 2323.0 4 2367.0 5 2596.0 6 3000.0 7 3627.0 8 4480.0 9 5714.0 10 6980.0 11 8859.0 12 11150.0 13 13135.0 14 14666.0 15 17177.0 16 20366.0 17 24151.0 18 21543.0 19 15912.0 20 16105.0 21 17436.0 22 19064.0 23 21559.0 24 24698.0 25 28460.0 26 32755.0 27 38801.0 28 45586.0 29 54026.0 30 65032.0 31 79668.0 32 98216.0 33 122482.0 34 158181.0 35 210514.0 36 286225.0 37 363924.0 38 288759.0 39 48184.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.9525636248353 18.483052666789817 14.483673562940853 22.08071014543403 2 15.295163613688162 20.424049501184975 40.3415687054046 23.939218179722268 3 20.357449203273138 24.69390348508435 29.5641715719868 25.38447573965571 4 12.550010509413756 16.5125815614492 38.13968395491712 32.797723974219934 5 13.410600611739646 37.758692001302116 34.01824504181704 14.812462345141201 6 30.837485486572724 36.26638138539717 17.763072755351182 15.13306037267893 7 28.093314068423116 32.99252054840536 20.863979474972187 18.050185908199342 8 26.03661063603116 33.5637744715098 19.788281636897697 20.61133325556134 9 25.942606860397827 17.60024509525809 21.187214197369762 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22.710025513746654 26.221387798965107 29.439264848301672 21.629321838986566 36-37 22.33980898888643 25.967014185143906 29.967727617643746 21.72544920832592 38-39 21.87589917119675 26.732898614788482 29.720983490195685 21.670218723819083 40-41 22.08874144535008 26.79341314647739 29.392370250694356 21.725475157478172 42-43 21.69218837429982 26.853203627421035 28.797771231772018 22.656836766507123 44-45 22.14840909219834 26.46841726088165 28.832275375390708 22.550898271529302 46-47 22.829350268082855 26.977038746930194 28.464320553079585 21.729290431907362 48-49 22.504723054085833 26.89862689937569 28.440046597472357 22.15660344906612 50-51 21.8134893495939 27.367325442664526 28.81173443895275 22.00745076878882 52-53 22.395425727176 27.681484555657338 27.805829842551184 22.117259874615478 54-55 21.988818717823975 27.101153435618908 28.158945933386097 22.751081913171024 56-57 22.562427147433286 27.160117603358465 27.805996663956595 22.471458585251657 58-59 23.125003545467646 26.89334212231126 27.777859717474445 22.20379461474665 60-61 22.41150290871944 27.198716433096276 28.715248642302615 21.674532015881667 62-63 22.0130181612562 27.35674508755696 28.604579818718722 22.025656932468117 64-65 21.84808888616594 27.916183059393212 28.003771285811048 22.2319567686298 66-67 22.12196003586112 27.410998999092577 27.535233143809133 22.931807821237165 68-69 22.008360624192168 27.3140348838898 28.00800027593418 22.669604215983853 70-71 22.632372360228644 27.109303854444583 27.724324826019775 22.533998959307 72-73 22.379841047430492 26.86123701632072 28.438827086607027 22.320094849641762 74-75 21.907938462264582 28.197904827039427 27.59554316815548 22.298613542540515 76-77 22.193921312528875 27.347331656608436 27.613725787405684 22.845021243457005 78-79 22.29514247178688 27.469632787918215 27.756473957763504 22.4787507825314 80-81 22.71756820826395 27.374883144984647 27.445330917289457 22.462217729461944 82-83 22.475317913264554 27.264785255804426 28.00713692332164 22.25275990760938 84-85 22.275355369684092 27.672554075578166 27.732711801829424 22.31937875290832 86-87 22.20102510153098 27.870943497785117 27.5122237695843 22.4158076310996 88-89 22.133950914799357 27.75454132050245 27.01821338873776 23.093294375960433 90-91 22.320369068856376 27.278738985995616 27.37659333868748 23.02429860646052 92-93 22.68481546310499 27.533988144232683 27.567643254918966 22.213553137743354 94-95 22.28575487203622 27.38910233267292 27.719816022072997 22.605326773217865 96-97 21.706833579006222 27.458461010623235 28.265301306302053 22.56940410406849 98-99 21.952587355387394 27.790926269642735 27.286055956289513 22.97043041868036 100-101 21.87222134069368 27.24919737393782 27.64316118102427 23.235420104344225 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 322.0 1 254.5 2 341.5 3 468.5 4 360.5 5 349.5 6 589.5 7 1160.5 8 1915.0 9 2412.5 10 2307.5 11 2050.5 12 2164.0 13 2332.0 14 2508.5 15 2662.0 16 2859.0 17 3068.5 18 3292.0 19 3742.0 20 4200.5 21 4859.0 22 5846.5 23 7040.5 24 8485.5 25 10532.0 26 13628.0 27 17430.5 28 20414.0 29 23410.5 30 27681.5 31 32218.5 32 37276.0 33 41649.0 34 45264.0 35 49617.5 36 54764.5 37 58773.5 38 60214.5 39 60929.5 40 62385.5 41 64197.5 42 65557.5 43 67727.0 44 70888.0 45 74244.5 46 77323.0 47 87035.0 48 108250.5 49 107224.5 50 85961.0 51 75758.5 52 71703.5 53 69925.5 54 67838.0 55 62815.5 56 56683.5 57 51217.5 58 47492.0 59 42882.5 60 35982.0 61 30041.5 62 24837.5 63 19113.0 64 13649.0 65 9962.5 66 7583.0 67 5961.0 68 4650.0 69 3575.0 70 2843.5 71 2178.0 72 1716.5 73 1453.0 74 1099.5 75 801.0 76 605.0 77 384.5 78 227.0 79 165.5 80 124.0 81 82.0 82 50.5 83 33.5 84 22.5 85 14.5 86 16.5 87 14.0 88 8.5 89 6.0 90 4.5 91 2.0 92 2.5 93 2.5 94 1.0 95 2.0 96 1.5 97 0.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013432790439846739 2 0.006398727878440508 3 0.00785092143950502 4 0.035215693855814426 5 0.04501800039299988 6 0.058632315027979685 7 0.02695634297726001 8 0.024596528440530178 9 0.015338794488743913 10-11 0.044654952002733755 12-13 0.04238589956357045 14-15 0.019513850976804387 16-17 0.02273590544041627 18-19 0.029679205904255972 20-21 0.023983884281956087 22-23 0.014226958793553894 24-25 0.0035170312807031156 26-27 0.0027682439757792266 28-29 0.0015656461830226775 30-31 0.006829847841881535 32-33 0.0011572167439732833 34-35 0.0020421471952469707 36-37 0.005944917390607847 38-39 0.006580252073573572 40-41 0.003766627049011079 42-43 6.353346829657242E-4 44-45 0.0011572167439732833 46-47 0.002110218768421869 48-49 4.5381048783266014E-4 50-51 0.0014521935610645124 52-53 9.756925488402193E-4 54-55 0.0026774818782126946 56-57 0.0024732671586879976 58-59 0.002064837719638604 60-61 7.260967805322562E-4 62-63 0.0011799072683649162 64-65 0.003199363939220254 66-67 0.0028363155489541255 68-69 0.006307965780873975 70-71 0.010142664403059954 72-73 0.0034262691831365836 74-75 0.0031539828904369877 76-77 0.0027682439757792266 78-79 0.035215693855814426 80-81 0.023257787501423828 82-83 0.004197747012452106 84-85 0.008554327695645642 86-87 0.00778284986633012 88-89 0.005854155293041315 90-91 0.013092432573972246 92-93 0.015043817671652682 94-95 0.006648323646748471 96-97 0.004129675439277208 98-99 0.023688907464864857 100-101 0.04308930581971108 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2203563.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.97059496643825 #Duplication Level Percentage of deduplicated Percentage of total 1 81.19568152610671 49.50549011352165 2 11.548188364451326 14.081998307301935 3 3.280216307917741 5.999902197370742 4 1.309618208638528 3.1939280543828845 5 0.6689398903612584 2.039283155605495 6 0.423808528777409 1.5503914890845694 7 0.2811947035502245 1.2001225863807892 8 0.19830653052028374 0.9672693721241475 9 0.1555640369474283 0.8536348699259116 >10 0.8379124861207482 9.519539338972526 >50 0.06545008120991555 2.727337464631189 >100 0.03131928364330674 3.4491995930868136 >500 0.00171374883072932 0.6620014352837831 >1k 0.0016392380120018783 2.0032221581689007 >5k 3.725540936367905E-4 1.6325158919106348 >10k+ 7.45108187273581E-5 0.6141639722482224 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13519 0.6135063985009732 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9176 0.4164165036352489 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8961 0.4066595781468467 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7267 0.3297840815079941 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5411 0.24555685496625237 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5120 0.232350969770322 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4375 0.1985420884267888 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3975 0.1803896689134824 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3368 0.15284337230203993 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3180 0.1443117351307859 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3118 0.14149811010622343 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2673 0.12130354339767004 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2527 0.11467791027531321 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2475 0.11231809573858337 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2232 0.10129050088424973 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.538104878326601E-5 0.0 8 0.0 0.0 0.0 4.538104878326601E-5 0.0 9 0.0 0.0 0.0 4.538104878326601E-5 0.0 10-11 0.0 0.0 0.0 9.076209756653203E-5 0.0 12-13 0.0 0.0 0.0 9.076209756653203E-5 0.0 14-15 0.0 0.0 0.0 9.076209756653203E-5 0.0 16-17 0.0 0.0 0.0 1.3614314634979803E-4 0.0 18-19 0.0 0.0 0.0 1.3614314634979803E-4 0.0 20-21 0.0 0.0 0.0 1.3614314634979803E-4 0.0 22-23 0.0 0.0 0.0 1.3614314634979803E-4 0.0 24-25 0.0 0.0 0.0 2.2690524391633004E-4 0.0 26-27 0.0 0.0 0.0 2.9497681709122906E-4 0.0 28-29 0.0 0.0 0.0 4.7650101222429314E-4 0.0 30-31 0.0 0.0 0.0 9.530020244485863E-4 0.0 32-33 0.0 0.0 0.0 0.0017017893293724755 0.0 34-35 0.0 0.0 0.0 0.0025640292562545295 0.0 36-37 0.0 0.0 0.0 0.004129675439277207 0.0 38-39 0.0 0.0 0.0 0.005105367988117426 6.807157317489902E-5 40-41 0.0 0.0 0.0 0.00664832364674847 9.076209756653203E-5 42-43 0.0 0.0 0.0 0.009325805524961164 9.076209756653203E-5 44-45 0.0 0.0 0.0 0.014068125122812462 9.076209756653203E-5 46-47 0.0 0.0 0.0 0.022894739111157702 9.076209756653203E-5 48-49 0.0 0.0 0.0 0.04054796708784818 9.076209756653203E-5 50-51 0.0 0.0 0.0 0.054321115393569416 9.076209756653203E-5 52-53 0.0 0.0 0.0 0.068752288906648 1.1345262195816503E-4 54-55 0.0 0.0 0.0 0.08833421145662729 1.3614314634979803E-4 56-57 0.0 0.0 0.0 0.10278807549409752 1.3614314634979803E-4 58-59 0.0 0.0 0.0 0.12386757265392456 1.3614314634979803E-4 60-61 0.0 0.0 0.0 0.15926479070487207 1.3614314634979803E-4 62-63 0.0 0.0 0.0 0.18436051068201817 1.3614314634979803E-4 64-65 0.0 0.0 0.0 0.21546921962294702 1.3614314634979803E-4 66-67 0.0 0.0 0.0 0.27519067982172507 1.3614314634979803E-4 68-69 0.0 0.0 0.0 0.3448278991796468 1.3614314634979803E-4 70-71 0.0 0.0 0.0 0.40044237446353925 1.8152419513306405E-4 72-73 0.0 0.0 0.0 0.4600503820403592 2.2690524391633004E-4 74-75 0.0 0.0 0.0 0.5126697081045561 2.7228629269959606E-4 76-77 0.0 0.0 0.0 0.5584138052780883 3.1766734148286205E-4 78-79 0.0 0.0 0.0 0.6216296062331779 3.1766734148286205E-4 80-81 0.0 0.0 0.0 0.7150238046291393 3.4035786587449505E-4 82-83 0.0 0.0 0.0 0.8221003892332555 3.630483902661281E-4 84-85 0.0 0.0 0.0 0.9435173852528835 3.630483902661281E-4 86-87 0.0 0.0 0.0 1.1408795664113076 3.630483902661281E-4 88-89 0.0 0.0 0.0 1.396011822670829 3.630483902661281E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1940 0.0 30.622381 2 GTACCTG 2115 0.0 29.438871 1 GAGTACT 8945 0.0 22.94594 12-13 GTACATG 10295 0.0 22.621946 1 TACATGG 10195 0.0 22.376114 2 ACATGGG 10115 0.0 21.096533 3 GTACTTT 9650 0.0 20.972265 14-15 GTATTAG 680 0.0 19.570835 1 CATGGGA 5295 0.0 19.297592 4 AGTACTT 9450 0.0 19.205873 12-13 GTGTAGC 1405 0.0 18.605726 1 ACCTGGG 3185 0.0 18.503033 3 AGAGTAC 15545 0.0 18.370073 10-11 ACTTTTT 11420 0.0 18.220331 16-17 CATGGGG 4595 0.0 16.445318 4 TACTTTT 10955 0.0 16.305664 14-15 GTATAGG 655 0.0 15.963996 1 GTATTGA 675 0.0 15.49099 1 ATGGGAG 2660 0.0 15.365527 5 GTATCAA 27665 0.0 15.341966 1 >>END_MODULE