FastQCFastQC Report
Thu 26 May 2016
SRR936730_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936730_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203563
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT161530.7330400809960959No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94800.4302123424653618No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83620.3794763299256704No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT77990.3539267994606916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52210.23693445569743185No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT48300.21919046562317485No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC47300.21465236074484823No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41640.18896668713351966No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC38480.17462627571800762No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35810.1625095356928756No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG30280.13741381571572947No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT29070.13192270881295431No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA27720.12579626722721338No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA27100.12298264220265089No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25710.11667467642177691No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC23550.10687236988459145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG18500.024.6445451
TACCTGG17550.024.6300242
GTACATG98350.022.1539121
GAGTACT111150.021.91795512-13
TACATGG96250.021.6652812
ACATGGG97650.020.6717783
AGTACTT115550.020.5696212-13
GTACTTT119200.020.33815814-15
GTGTAGC13250.019.5590081
AGAGTAC177400.018.72519110-11
TATACCG1559.639049E-418.3840525
ACTTTTT135850.018.14405416-17
TACTTTT130700.018.00363514-15
CATGGGA49300.017.2443774
ACTATAC7000.016.9630173
CATGGGG47550.015.5817244
GTATAGG6600.015.2701851
ATGGGAT21450.015.0557825
GTGTACG2951.9892406E-514.641631
TGTACGC1950.00446270314.615622
Produced by FastQC (version 0.11.5)