##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936730_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2203563 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42586120750802 31.0 31.0 34.0 30.0 34.0 2 31.639492040844758 31.0 31.0 34.0 30.0 34.0 3 31.907950442079485 31.0 31.0 34.0 30.0 34.0 4 35.719093577084024 37.0 35.0 37.0 33.0 37.0 5 35.521631103807785 37.0 35.0 37.0 33.0 37.0 6 35.63067994879202 37.0 35.0 37.0 33.0 37.0 7 35.566370010750774 37.0 35.0 37.0 33.0 37.0 8 35.549423819514125 37.0 35.0 37.0 33.0 37.0 9 37.16068975563667 39.0 37.0 39.0 33.0 39.0 10-11 37.04333073299924 39.0 37.0 39.0 33.0 39.0 12-13 36.92060744349038 39.0 37.0 39.0 33.0 39.0 14-15 37.99783736612024 40.0 37.0 41.0 33.0 41.0 16-17 37.92592292573437 40.0 37.0 41.0 33.0 41.0 18-19 38.00959899943864 40.0 37.0 41.0 33.0 41.0 20-21 38.093207228474974 40.0 37.0 41.0 33.0 41.0 22-23 38.00492815499262 40.0 37.0 41.0 33.0 41.0 24-25 37.890376630938164 40.0 37.0 41.0 33.0 41.0 26-27 37.66880819835875 39.5 37.0 41.0 32.0 41.0 28-29 37.58803537725039 39.0 37.0 41.0 32.0 41.0 30-31 37.5676241160339 39.0 37.0 41.0 32.0 41.0 32-33 37.377071815055885 39.0 36.0 41.0 32.0 41.0 34-35 37.19035738937348 39.0 36.0 41.0 31.0 41.0 36-37 36.9447270171082 39.0 35.5 41.0 31.0 41.0 38-39 36.78337288291735 39.0 35.0 41.0 31.0 41.0 40-41 36.562200173083326 39.0 35.0 40.5 30.0 41.0 42-43 36.306105838589595 39.0 35.0 40.0 30.0 41.0 44-45 36.01783021406695 39.0 35.0 40.0 29.5 41.0 46-47 35.60710971276973 38.0 35.0 40.0 27.5 41.0 48-49 35.13447085470213 38.0 34.0 40.0 26.0 41.0 50-51 34.92591294190363 38.0 34.0 40.0 26.0 41.0 52-53 34.671127850667304 38.0 34.0 40.0 25.5 41.0 54-55 34.4080130679268 38.0 33.5 40.0 24.0 41.0 56-57 34.11234577817834 38.0 33.0 40.0 23.5 41.0 58-59 33.74831897250044 37.0 33.0 40.0 23.0 41.0 60-61 33.3488763425416 37.0 32.0 39.5 22.0 41.0 62-63 33.0709975162952 36.0 32.0 39.0 22.0 40.5 64-65 32.64109376496157 36.0 31.0 39.0 20.5 40.0 66-67 32.21379012081796 35.0 31.0 38.5 19.5 40.0 68-69 31.746047877914087 35.0 31.0 38.0 18.0 40.0 70-71 31.25829622298069 35.0 30.0 37.5 17.0 39.5 72-73 30.824321110855465 34.0 30.0 37.0 13.0 39.0 74-75 30.460693204596375 34.0 29.5 36.0 11.0 39.0 76-77 29.046761313382007 32.5 28.0 35.0 8.0 37.0 78-79 29.847876598036905 34.0 29.0 35.0 7.5 37.5 80-81 29.67161410860502 34.0 29.0 35.0 7.0 37.0 82-83 29.30618457470923 34.0 29.0 35.0 2.0 37.0 84-85 28.846856885870746 34.0 29.0 35.0 2.0 36.0 86-87 28.476726556036745 33.0 28.5 35.0 2.0 36.0 88-89 28.031090556521416 33.0 27.0 35.0 2.0 35.0 90-91 27.54801814152806 33.0 26.5 35.0 2.0 35.0 92-93 27.06755196016633 33.0 25.5 34.5 2.0 35.0 94-95 26.507523270267292 32.0 25.0 34.0 2.0 35.0 96-97 25.7920687994852 32.0 23.5 34.0 2.0 35.0 98-99 24.899286292245783 32.0 19.0 34.0 2.0 35.0 100-101 22.077620199649388 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.30835733302526336 1101 2 0.3714169711563713 1101 3 0.20304568581004645 1101 4 0.1295933273370622 1101 5 0.2271438373154453 1101 6 0.18923589559571496 1101 7 0.18790755161193573 1101 8 0.09347717702088687 1101 9 0.14654534535643648 1101 10-11 0.17908781266397256 1101 12-13 0.19757566486715206 1101 14-15 0.32286567578803727 1101 16-17 0.27618112279981943 1101 18-19 0.24223310473212933 1101 20-21 0.16384096733516174 1101 22-23 0.10454956791662084 1101 24-25 0.043892330519788914 1101 26-27 0.20027369211723567 1101 28-29 0.19803034870504632 1101 30-31 0.036116075594357255 1101 32-33 0.14972668889999596 1101 34-35 0.22327131621484853 1101 36-37 0.2979930144190277 1101 38-39 0.18731799854852937 1101 40-41 0.1912427068979099 1101 42-43 0.3085355713482443 1101 44-45 0.352734892063431 1101 46-47 0.3902051803791622 1101 48-49 0.4593821284779054 1101 50-51 0.40128994265345597 1101 52-53 0.326898876755358 1101 54-55 0.24823754055609015 1101 56-57 0.29414150545488127 1101 58-59 0.20742482744430646 1101 60-61 0.32477781668383443 1101 62-63 0.38700892919799657 1101 64-65 0.30075294532505126 1101 66-67 0.060177258156059565 1101 68-69 0.18862408990248625 1101 70-71 0.17096923660968244 1101 72-73 -0.010464501604154464 1101 74-75 0.19097093857454084 1101 76-77 0.6195588908222192 1101 78-79 0.49394435120683156 1101 80-81 0.6148848028813916 1101 82-83 0.46150560955207354 1101 84-85 0.4573489407207134 1101 86-87 0.45677032229880865 1101 88-89 0.36122033654004326 1101 90-91 0.3362712817869742 1101 92-93 0.34480079648605155 1101 94-95 0.1335672218401207 1101 96-97 0.1646692747089169 1101 98-99 0.014531113346503588 1101 100-101 -0.051106547869352426 1102 1 0.46512833265668974 1102 2 0.5161255304840004 1102 3 0.33555628525489567 1102 4 0.11917731302948198 1102 5 0.21525729868740484 1102 6 0.166376206613144 1102 7 0.1763107031950284 1102 8 0.05783758107456549 1102 9 0.21861215924664634 1102 10-11 0.24630595161740132 1102 12-13 0.30412211608506823 1102 14-15 0.28835934520992623 1102 16-17 0.33288023887678975 1102 18-19 0.29005765615719525 1102 20-21 0.26212534422058553 1102 22-23 0.1860398691978702 1102 24-25 0.21745116021924815 1102 26-27 0.04607876226059915 1102 28-29 0.18219851344483828 1102 30-31 0.26736979938834793 1102 32-33 0.38821171689540535 1102 34-35 0.5972757520855154 1102 36-37 0.5383819898328355 1102 38-39 0.36771543366938175 1102 40-41 0.4185984461349648 1102 42-43 0.48443442377803336 1102 44-45 0.3326086945888065 1102 46-47 0.3940036641340967 1102 48-49 0.452749454638969 1102 50-51 0.4036587975164352 1102 52-53 0.14876292460882468 1102 54-55 0.26304989898347486 1102 56-57 0.37928176650802214 1102 58-59 0.3107461009039909 1102 60-61 0.4808403329607245 1102 62-63 0.2637205055473757 1102 64-65 0.2875653674055698 1102 66-67 0.31098490009142665 1102 68-69 0.26875440429764197 1102 70-71 0.0020140514971238588 1102 72-73 0.1719857471392956 1102 74-75 -0.24483333419351894 1102 76-77 0.09512079229880044 1102 78-79 0.408516538824788 1102 80-81 0.3761315590525811 1102 82-83 0.4652240135557868 1102 84-85 0.5611738781712425 1102 86-87 0.4374868444283706 1102 88-89 0.48347662117496526 1102 90-91 0.4546597828822563 1102 92-93 0.6312466654831077 1102 94-95 0.4421147599524353 1102 96-97 0.4230032154848047 1102 98-99 -0.04332274247640555 1102 100-101 -0.31666879841151996 1103 1 0.44627081212477293 1103 2 0.4514737819709289 1103 3 0.24337161423766673 1103 4 0.10986885777738564 1103 5 -1.913917219908967E-4 1103 6 0.11047270683243227 1103 7 0.14115824189549642 1103 8 0.24643717927241227 1103 9 0.029793191078688608 1103 10-11 0.11664743034689451 1103 12-13 0.19408127709615286 1103 14-15 0.262919812915996 1103 16-17 0.09139696268419328 1103 18-19 0.12720125670206528 1103 20-21 0.08658348628969037 1103 22-23 0.12360737483021467 1103 24-25 0.058509154039384725 1103 26-27 0.11816336937512517 1103 28-29 0.13921527433937086 1103 30-31 0.17156544089534265 1103 32-33 0.11094516628573814 1103 34-35 0.14044633513670846 1103 36-37 0.3866206995391579 1103 38-39 0.3498997545575335 1103 40-41 0.4132785584218581 1103 42-43 0.5379552205324245 1103 44-45 0.46570669472122717 1103 46-47 0.48245390158300694 1103 48-49 0.566092126960136 1103 50-51 0.29341875001836115 1103 52-53 0.3140548783273971 1103 54-55 0.3309258218681279 1103 56-57 0.46026363463415265 1103 58-59 0.5126585689671614 1103 60-61 0.4088986813902622 1103 62-63 0.20416595601829357 1103 64-65 0.23315315198292552 1103 66-67 0.2661931032671845 1103 68-69 0.3052975915574585 1103 70-71 0.35310282846639396 1103 72-73 0.18778785219494054 1103 74-75 0.2951602838329208 1103 76-77 0.19756897444025867 1103 78-79 0.2966569369331644 1103 80-81 0.28483955123822824 1103 82-83 0.42691051727470963 1103 84-85 0.41640836591830066 1103 86-87 0.21172594010636203 1103 88-89 0.24424503999704328 1103 90-91 0.08420646197435389 1103 92-93 0.2228965184869729 1103 94-95 0.21648693786645978 1103 96-97 -0.05882956585593746 1103 98-99 -0.023479965365421407 1103 100-101 0.045816099160788326 1104 1 0.2871060241569232 1104 2 0.2944264838031323 1104 3 0.04750124097855135 1104 4 -0.026732169520627735 1104 5 0.020266139748905232 1104 6 0.12368387589148 1104 7 -0.005149589157070977 1104 8 -0.052264672136011825 1104 9 -0.021680010181043485 1104 10-11 0.01752052436595619 1104 12-13 -0.08474834570351675 1104 14-15 0.061445137842298436 1104 16-17 0.00123953948065747 1104 18-19 2.4716981592831644E-4 1104 20-21 -0.058596297486893434 1104 22-23 -0.01829673320266778 1104 24-25 1.4761124429440997E-4 1104 26-27 0.07197163843194687 1104 28-29 -0.06747918614039605 1104 30-31 -0.1073849021721216 1104 32-33 -0.05067087238713697 1104 34-35 0.07741710758413234 1104 36-37 0.160070735755248 1104 38-39 -0.029632264796127572 1104 40-41 0.10439190730144787 1104 42-43 0.044883553761422945 1104 44-45 -0.035556396969539605 1104 46-47 -0.0022005486562406418 1104 48-49 0.07505142551742239 1104 50-51 0.01414069033916121 1104 52-53 -0.03711781392004099 1104 54-55 0.22043249824540823 1104 56-57 0.11456698926657793 1104 58-59 -0.009178965729567778 1104 60-61 -0.1001511987671364 1104 62-63 -0.30968094021228154 1104 64-65 -0.21556325540839083 1104 66-67 -0.027968407534544326 1104 68-69 -0.13939522090021228 1104 70-71 -0.060515527240756484 1104 72-73 -0.029447276111326914 1104 74-75 -0.09302070515918004 1104 76-77 -0.009689509677489383 1104 78-79 0.048942093771810136 1104 80-81 0.05275616118790083 1104 82-83 0.06168774118546949 1104 84-85 -0.08915097629037305 1104 86-87 -0.02037425804709514 1104 88-89 0.055638085648514135 1104 90-91 -0.0953882027528401 1104 92-93 -0.038157003481302354 1104 94-95 -0.0659355618743831 1104 96-97 -0.19690573189945937 1104 98-99 -0.20029251643141777 1104 100-101 -0.12010943779600325 1105 1 0.28494522707480385 1105 2 0.305753912923759 1105 3 0.062236751104940424 1105 4 0.002512985776604637 1105 5 -0.17854327714418616 1105 6 -0.07709867580612695 1105 7 -0.02674884708680736 1105 8 0.020419608869481465 1105 9 -0.012467467906766672 1105 10-11 -0.07374835412405645 1105 12-13 -0.10274951457141412 1105 14-15 0.023464471078220583 1105 16-17 0.11384193271087639 1105 18-19 -0.014768660837631842 1105 20-21 0.017211890218270298 1105 22-23 -0.02967560189402718 1105 24-25 -0.15669951504303015 1105 26-27 -0.017539916872792105 1105 28-29 -0.024200913812940428 1105 30-31 -0.04588613068981573 1105 32-33 -0.1147267099574023 1105 34-35 -0.11077699150958864 1105 36-37 -0.11644024717955404 1105 38-39 -0.13084238488657718 1105 40-41 -0.24452638722377174 1105 42-43 -0.10275454891932156 1105 44-45 -0.4397908287080554 1105 46-47 -0.12755459364960586 1105 48-49 -0.026638071158949117 1105 50-51 -0.19536262793204173 1105 52-53 -0.28235253251375525 1105 54-55 -0.10577614735572638 1105 56-57 -0.0049566233395026416 1105 58-59 -0.14741418296875963 1105 60-61 -0.0921668390775281 1105 62-63 -0.06055842891568375 1105 64-65 -0.11750368335881234 1105 66-67 -0.0848376176506207 1105 68-69 -0.3410361599622469 1105 70-71 -0.10116954368265496 1105 72-73 -0.39490850307410597 1105 74-75 -0.3967685219096495 1105 76-77 -0.29182784760631364 1105 78-79 -0.21405251361860067 1105 80-81 -0.3306140790600267 1105 82-83 -0.24707350682229645 1105 84-85 -0.06268305514606354 1105 86-87 -0.2957497166467853 1105 88-89 -0.27869767701768566 1105 90-91 -0.5851678135144667 1105 92-93 -0.4327057159170131 1105 94-95 -0.34179006194877815 1105 96-97 -0.4201650302302049 1105 98-99 -0.3780276271052472 1105 100-101 -0.30461446513437807 1106 1 0.39259523785766604 1106 2 0.37640349106908033 1106 3 0.1691604071170829 1106 4 0.022395789955858447 1106 5 -0.09403011345239776 1106 6 -0.018521272716270687 1106 7 0.011594481893688169 1106 8 0.05576225912503219 1106 9 0.08809859928568642 1106 10-11 0.0828462592077912 1106 12-13 0.09001862478285716 1106 14-15 0.17190032760132112 1106 16-17 0.17960383123554635 1106 18-19 0.017003902797789294 1106 20-21 0.10453819021470423 1106 22-23 0.12426811421106265 1106 24-25 0.0949513213233999 1106 26-27 0.011923096586599513 1106 28-29 0.0461503164256456 1106 30-31 -0.03604941497766134 1106 32-33 0.08298963858175767 1106 34-35 -0.12104783319467316 1106 36-37 0.005092864145886722 1106 38-39 0.06509264142228233 1106 40-41 0.09708080831913435 1106 42-43 0.04562077803954878 1106 44-45 -0.06112300172602403 1106 46-47 -0.11004005001606032 1106 48-49 -0.16093498223275304 1106 50-51 -0.2020769442654995 1106 52-53 -0.022003300081550492 1106 54-55 -0.225760958735016 1106 56-57 -0.13212808011081023 1106 58-59 -0.20748679860007258 1106 60-61 0.01851791898282329 1106 62-63 -0.4926774994991092 1106 64-65 -0.2948156150259038 1106 66-67 -0.18945767721434947 1106 68-69 -0.10270860835996842 1106 70-71 0.014237181912683639 1106 72-73 0.07692044236196338 1106 74-75 -0.08072613666113426 1106 76-77 -0.13663153336063516 1106 78-79 0.02027198305988165 1106 80-81 -0.048121610525477365 1106 82-83 0.17125243065827433 1106 84-85 0.11125857053911048 1106 86-87 0.06509794201674879 1106 88-89 -0.043472513654013056 1106 90-91 -0.25423895923658435 1106 92-93 0.20560549601055556 1106 94-95 0.3026179324520726 1106 96-97 0.3671021624813733 1106 98-99 0.2956566652412995 1106 100-101 0.2470167570071382 1107 1 0.5141540103396807 1107 2 0.5511628187410018 1107 3 0.3409905457565401 1107 4 0.17489974864220414 1107 5 0.03547322529392716 1107 6 0.151760485796693 1107 7 0.07487944373632871 1107 8 0.19408881060117977 1107 9 0.2653174755957082 1107 10-11 0.3278871065302056 1107 12-13 0.2578490111043408 1107 14-15 0.36227308537161207 1107 16-17 0.37360417519752076 1107 18-19 0.40406007333238136 1107 20-21 0.25404176047389626 1107 22-23 0.34325549528969646 1107 24-25 0.2756784444526019 1107 26-27 0.16110695859610757 1107 28-29 0.25196250216070126 1107 30-31 0.23492335433152078 1107 32-33 0.2151559265595111 1107 34-35 0.16311887297617034 1107 36-37 0.2597439760875133 1107 38-39 0.2073651092870037 1107 40-41 0.2487638150171989 1107 42-43 0.22421090875612748 1107 44-45 0.13467221007334018 1107 46-47 0.35835835129569915 1107 48-49 0.18703804909021216 1107 50-51 -0.007095151099093755 1107 52-53 -0.2349447960442248 1107 54-55 -0.005212243833561558 1107 56-57 0.19093563627343002 1107 58-59 -0.06248725611360584 1107 60-61 -0.05313228270898662 1107 62-63 -0.026191192382960082 1107 64-65 -0.02126607072039377 1107 66-67 0.05885457558593998 1107 68-69 0.17027988471540922 1107 70-71 -0.10168030175555387 1107 72-73 0.14823687966552157 1107 74-75 0.19869630119879034 1107 76-77 0.08855414547368667 1107 78-79 0.19758153524416855 1107 80-81 0.17778534114455624 1107 82-83 0.03029131783837613 1107 84-85 0.21996268275891495 1107 86-87 0.4528317470483678 1107 88-89 0.42921264243894797 1107 90-91 0.3207068097922168 1107 92-93 0.4333702465958602 1107 94-95 0.566262951930387 1107 96-97 0.70737656402979 1107 98-99 0.7835738064174826 1107 100-101 0.6983781078075673 1108 1 0.37032818229959474 1108 2 0.36445817766391286 1108 3 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-0.5116483596072499 2316 56-57 -0.608635289341656 2316 58-59 -0.6614418900135846 2316 60-61 -0.6232441012536611 2316 62-63 -0.7929224332695028 2316 64-65 -0.6844187248317795 2316 66-67 -0.8392314959741967 2316 68-69 -1.0145321125241118 2316 70-71 -0.9808462025331401 2316 72-73 -0.9405904943573873 2316 74-75 -1.0036586532777676 2316 76-77 -0.8025497912844237 2316 78-79 -0.7313216435169885 2316 80-81 -0.5920939244793928 2316 82-83 -0.6184704380862023 2316 84-85 -0.8336597645611548 2316 86-87 -0.8533045184235384 2316 88-89 -1.018094315061333 2316 90-91 -1.0621219948988845 2316 92-93 -1.2267242723110705 2316 94-95 -1.2669878040620155 2316 96-97 -1.3346489988892252 2316 98-99 -1.7273828829648856 2316 100-101 -2.0810990402055722 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 26.0 5 60.0 6 178.0 7 423.0 8 998.0 9 2005.0 10 3196.0 11 5097.0 12 7382.0 13 9967.0 14 12741.0 15 16217.0 16 20162.0 17 25010.0 18 21806.0 19 15502.0 20 14135.0 21 14421.0 22 15730.0 23 17594.0 24 20780.0 25 24888.0 26 30713.0 27 38071.0 28 46902.0 29 57614.0 30 70907.0 31 86306.0 32 105777.0 33 133130.0 34 171618.0 35 227804.0 36 315429.0 37 403852.0 38 250913.0 39 16199.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21350000320966 19.593171315932096 15.296861229995809 21.896467450862435 2 15.463018687406446 21.43169660809095 39.31676184199897 23.78852286250364 3 20.414774380146163 24.812624491539 30.039034011462867 24.733567116851972 4 13.075120535161139 17.340134044448803 37.3607939730377 32.223951447352356 5 13.197943512393337 38.15420752662846 33.989906347129626 14.65794261384857 6 30.826166531204237 37.013554865461074 17.14564094604965 15.014637657285043 7 27.88107260831662 33.27819535906166 20.655638164191355 18.185093868430354 8 26.16467058123593 33.94284619954138 19.424677215945266 20.46780600327742 9 25.673415113434718 17.548276128107034 20.956355203383755 35.8219535550745 10-11 25.087659466711866 26.742689258337265 26.860929983383674 21.3087212915672 12-13 26.76423605438472 24.166712047777235 27.33783060139048 21.73122129644757 14-15 22.91837034764622 25.7525996545593 26.98701012270134 24.342019875093147 16-17 21.439323495629576 28.766366107980573 27.525375947953385 22.268934448436465 18-19 21.2035916377249 27.70581099791565 29.95972885730973 21.13086850704972 20-21 22.640774346263004 25.984837277099533 29.83616980143967 21.538218575197792 22-23 21.828024281017928 26.127281110508036 30.073354959220016 21.971339649254016 24-25 22.292577974852545 26.221533035361368 30.14570039522355 21.340188594562534 26-27 21.931831280445024 25.89967970041233 30.82225505794709 21.346233961195555 28-29 21.24772470766663 27.257763903278466 30.22012531522811 21.274386073826797 30-31 21.744284143453125 26.135331733197553 30.06696427558459 22.053419847764733 32-33 21.699599272063864 26.31253148414561 30.185342343282702 21.802526900507832 34-35 22.575314316884786 26.158580126554966 29.926343804095985 21.339761752464266 36-37 22.332183123248107 25.89109514113956 30.356878263116965 21.419843472495366 38-39 21.805114539602037 26.66100461934115 30.222126821086416 21.3117540199704 40-41 21.981627877267158 26.677857945637882 29.726687274776076 21.613826902318877 42-43 21.684592815788537 26.77181132055776 29.159816288457712 22.38377957519599 44-45 22.175640314659322 26.327259129231965 29.1481697969527 22.34893075915601 46-47 22.844226469278826 27.053255415329975 28.636953809666654 21.46556430572454 48-49 22.666267578035026 26.96051722975909 28.53230314609072 21.84091204611516 50-51 21.891370559988165 27.376959645445737 28.93147007189063 21.80019972267547 52-53 22.566271580454522 27.67011076557928 27.849641086848248 21.913976567117952 54-55 22.0936884674307 27.193818700613676 28.218868902453554 22.49362392950207 56-57 22.695446964453875 27.209453249980143 27.907986248993065 22.187113536572912 58-59 23.318284813656575 26.902609847159503 27.799122230194246 21.979983108989675 60-61 22.471054456470043 27.227622344616254 28.809370586462673 21.491952612451033 62-63 22.128764589329492 27.26510714347305 28.67411937586397 21.932008891333492 64-65 22.017983849847425 27.888561133152294 28.08341416276968 22.0100408542306 66-67 22.33754398974539 27.332673321695765 27.61783374741008 22.71194894114876 68-69 22.139933625557564 27.322844543758528 28.03920838970048 22.498013440983428 70-71 22.789280501884242 27.181221454970554 27.719243071136574 22.310254972008625 72-73 22.56098418788117 26.840394397618766 28.47032283624294 22.128298578257123 74-75 22.034995141958728 28.2662215693402 27.562883384772753 22.13589990392832 76-77 22.387794185719343 27.39457872332701 27.57354075714532 22.64408633380833 78-79 22.47276019783534 27.494210513775062 27.74659920870403 22.28643007968557 80-81 22.851542022373383 27.371855941446317 27.50202769281464 22.274574343365668 82-83 22.620759048773365 27.297966004538143 27.99552584992626 22.085749096762232 84-85 22.252645012780295 27.78311802892994 27.691826964970375 22.272409993319393 86-87 22.279470932657514 27.84565677067047 27.421824685966676 22.45304761070534 88-89 22.18372882125308 27.787128769050945 27.01478682236521 23.01435558733077 90-91 22.420937934834832 27.290861112264807 27.32311165792543 22.96508929497493 92-93 22.720401433717782 27.531493050607626 27.576807282226202 22.171298233448393 94-95 22.341494962330945 27.381387855132978 27.651130071707364 22.625987110828717 96-97 21.771872288202978 27.444248176401604 28.15344550263782 22.630434032757602 98-99 22.024056577548482 27.740721788097428 27.16062718204913 23.074594452304957 100-101 21.765074443221796 27.406415807755874 27.437505205204776 23.391004543817555 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 539.0 1 417.0 2 463.5 3 622.5 4 485.0 5 407.0 6 640.0 7 1249.0 8 1959.5 9 2382.5 10 2314.0 11 2112.5 12 2199.5 13 2401.0 14 2677.5 15 2934.0 16 3183.5 17 3473.5 18 3751.5 19 4087.0 20 4683.0 21 5371.5 22 6258.5 23 7576.5 24 9036.0 25 11013.5 26 14072.5 27 17885.0 28 20894.0 29 24171.0 30 28599.5 31 32792.0 32 37583.0 33 41794.0 34 45468.0 35 49361.0 36 54472.5 37 58869.5 38 60107.5 39 61195.5 40 62738.0 41 64229.0 42 65140.0 43 67163.5 44 70245.0 45 73545.0 46 76316.5 47 86780.5 48 110152.5 49 108608.0 50 85218.0 51 74140.5 52 70411.5 53 69146.0 54 67372.5 55 62747.5 56 56674.0 57 51017.5 58 47184.5 59 42482.0 60 35459.0 61 29378.0 62 24300.0 63 18597.5 64 13145.0 65 9541.5 66 7239.5 67 5762.5 68 4530.0 69 3616.5 70 2809.5 71 2025.0 72 1605.5 73 1427.0 74 1108.0 75 798.5 76 602.0 77 369.5 78 214.0 79 162.5 80 118.5 81 80.5 82 52.5 83 33.5 84 20.0 85 10.0 86 4.5 87 3.0 88 2.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0276538496970589 2 0.011844453732432429 3 0.004538104878326601 4 0.011980596878782227 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004220437536843738 10-11 3.403578658744951E-4 12-13 1.3614314634979803E-4 14-15 4.538104878326601E-5 16-17 0.0 18-19 0.0 20-21 4.538104878326601E-5 22-23 0.0014068125122812462 24-25 0.0 26-27 4.538104878326601E-5 28-29 0.0 30-31 0.0 32-33 0.0033128165611784185 34-35 6.580252073573571E-4 36-37 0.0023825050611214656 38-39 9.076209756653203E-5 40-41 0.0027909345001708597 42-43 0.009870378110360357 44-45 0.0016790988049808424 46-47 0.0022690524391633005 48-49 0.00950732972009423 50-51 3.403578658744951E-4 52-53 0.0016110272318059433 54-55 0.009416567622527698 56-57 0.003993532292927409 58-59 0.0010891451707983842 60-61 0.005218820610075591 62-63 0.003131292366045355 64-65 0.01651870175710883 66-67 0.00301783974408719 68-69 1.8152419513306405E-4 70-71 0.0012252883171481822 72-73 0.0 74-75 0.0 76-77 0.0010437641220151183 78-79 0.009779616012793825 80-81 0.014521935610645124 82-83 0.010868761183592208 84-85 0.007760159341938489 86-87 0.009711544439618925 88-89 0.026684056684560416 90-91 0.02266783386724137 92-93 0.0027682439757792266 94-95 0.00739711095167236 96-97 0.010301498073801384 98-99 0.009779616012793825 100-101 0.010868761183592208 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2203563.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.95083176961625 #Duplication Level Percentage of deduplicated Percentage of total 1 79.7340100527078 47.00386212934069 2 12.389128148489434 14.606988185076359 3 3.5508489465177018 6.2797649665645245 4 1.4135371368892515 3.3331675982745312 5 0.7566818975202816 2.2303513621916062 6 0.4524281966619191 1.6002611105548588 7 0.31454083433498214 1.2979710666689388 8 0.21017455903164675 0.991197205738231 9 0.1605754107140972 0.8519448621009402 >10 0.9112852449793531 10.022694053603624 >50 0.06790422242269722 2.7352239803384824 >100 0.03471357934296373 3.7190154186430324 >500 0.0022403952083399274 0.9261971985348928 >1k 0.0015451001436826452 2.260181667349573 >5k 3.0902002873652907E-4 1.4055316881144218 >10k+ 7.725500718413227E-5 0.7356475069053288 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16153 0.7330400809960959 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9480 0.4302123424653618 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8362 0.3794763299256704 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7799 0.3539267994606916 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5221 0.23693445569743185 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4830 0.21919046562317485 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4730 0.21465236074484823 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4164 0.18896668713351966 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3848 0.17462627571800762 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3581 0.1625095356928756 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3028 0.13741381571572947 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2907 0.13192270881295431 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2772 0.12579626722721338 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2710 0.12298264220265089 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2571 0.11667467642177691 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2355 0.10687236988459145 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 2.2690524391633006E-5 24-25 0.0 0.0 0.0 9.076209756653203E-5 4.538104878326601E-5 26-27 0.0 0.0 0.0 1.3614314634979803E-4 4.538104878326601E-5 28-29 0.0 0.0 0.0 2.9497681709122906E-4 4.538104878326601E-5 30-31 0.0 0.0 0.0 7.260967805322561E-4 4.538104878326601E-5 32-33 0.0 0.0 0.0 0.0014521935610645122 9.076209756653203E-5 34-35 0.0 0.0 0.0 0.0023825050611214656 9.076209756653203E-5 36-37 0.0 0.0 0.0 0.003948151244144143 9.076209756653203E-5 38-39 0.0 0.0 0.0 0.005150749036900692 9.076209756653203E-5 40-41 0.0 0.0 0.0 0.006693704695531736 9.076209756653203E-5 42-43 0.0 0.0 0.0 0.00937118657374443 9.076209756653203E-5 44-45 0.0 0.0 0.0 0.014158887220378995 9.076209756653203E-5 46-47 0.0 0.0 0.0 0.022985501208724234 9.076209756653203E-5 48-49 0.0 0.0 0.0 0.04029837131954021 9.076209756653203E-5 50-51 0.0 0.0 0.0 0.05382192385695349 9.076209756653203E-5 52-53 0.0 0.0 0.0 0.06845731208955677 9.076209756653203E-5 54-55 0.0 0.0 0.0 0.08812999673710259 9.076209756653203E-5 56-57 0.0 0.0 0.0 0.10260655129896445 9.076209756653203E-5 58-59 0.0 0.0 0.0 0.12357259583683333 9.076209756653203E-5 60-61 0.0 0.0 0.0 0.1591513380829139 9.076209756653203E-5 62-63 0.0 0.0 0.0 0.18436051068201817 9.076209756653203E-5 64-65 0.0 0.0 0.0 0.2151061712326809 9.076209756653203E-5 66-67 0.0 0.0 0.0 0.275009155626592 9.076209756653203E-5 68-69 0.0 0.0 0.0 0.34439677921620576 9.076209756653203E-5 70-71 0.0 0.0 0.0 0.4004650649879309 9.076209756653203E-5 72-73 0.0 0.0 0.0 0.4597100241744847 9.076209756653203E-5 74-75 0.0 0.0 0.0 0.5126243270557729 9.076209756653203E-5 76-77 0.0 0.0 0.0 0.5586860915707879 9.076209756653203E-5 78-79 0.0 0.0 0.0 0.6219245830502691 9.076209756653203E-5 80-81 0.0 0.0 0.0 0.7155910677389301 1.1345262195816503E-4 82-83 0.0 0.0 0.0 0.8226676523430463 1.3614314634979803E-4 84-85 0.0 0.0 0.0 0.9440846483626744 1.3614314634979803E-4 86-87 0.0 0.0 0.0 1.1424225220699387 1.3614314634979803E-4 88-89 0.0 0.0 0.0 1.3987346855978249 1.3614314634979803E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCTG 1850 0.0 24.644545 1 TACCTGG 1755 0.0 24.630024 2 GTACATG 9835 0.0 22.153912 1 GAGTACT 11115 0.0 21.917955 12-13 TACATGG 9625 0.0 21.665281 2 ACATGGG 9765 0.0 20.671778 3 AGTACTT 11555 0.0 20.56962 12-13 GTACTTT 11920 0.0 20.338158 14-15 GTGTAGC 1325 0.0 19.559008 1 AGAGTAC 17740 0.0 18.725191 10-11 TATACCG 155 9.639049E-4 18.384052 5 ACTTTTT 13585 0.0 18.144054 16-17 TACTTTT 13070 0.0 18.003635 14-15 CATGGGA 4930 0.0 17.244377 4 ACTATAC 700 0.0 16.963017 3 CATGGGG 4755 0.0 15.581724 4 GTATAGG 660 0.0 15.270185 1 ATGGGAT 2145 0.0 15.055782 5 GTGTACG 295 1.9892406E-5 14.64163 1 TGTACGC 195 0.004462703 14.61562 2 >>END_MODULE