FastQCFastQC Report
Thu 26 May 2016
SRR936729_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936729_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219297
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT139080.6266849367164466No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT90350.40711090043378595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89590.40368639258287653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70800.31901994190052074No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC54990.24778116673883666No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT52210.2352546774947202No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41310.18614002542246486No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40150.18091314501844502No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC32470.14630759199872753No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT31480.14184671992977957No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC30480.13734078854700385No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27570.12422852822312651No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA24820.1118372169204933No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC24710.11134156446838796No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA22650.10205934581986999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG18750.025.3462352
GTACCTG20500.024.5729311
GTACATG101900.024.0647011
TACATGG102150.023.773732
ACATGGG101250.022.8116133
GAGTACT89750.022.05365812-13
GTACACG3301.8189894E-1221.6014211
AGTACTT94100.021.38768812-13
GTACTTT95750.020.5722114-15
GTGTAGC14450.020.3905451
TAGCGCG1404.837057E-420.3675124
GTATAGG6100.020.2557581
CATGGGG46950.019.940934
AGAGTAC157150.019.836510-11
CGTATAG1300.007544086318.2781241
TACTTTT110900.018.01895714-15
CATGGGA53450.017.7826734
ACTTTTT114000.017.71515716-17
ATGGGGA26100.016.9331465
TAGCCCT17200.016.854514
Produced by FastQC (version 0.11.5)