##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936729_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2219297 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.189273450106047 31.0 31.0 34.0 28.0 34.0 2 31.21793928437699 31.0 31.0 34.0 28.0 34.0 3 31.261987467202452 31.0 31.0 34.0 27.0 34.0 4 34.88210591011478 37.0 35.0 37.0 32.0 37.0 5 34.8592054150481 37.0 35.0 37.0 32.0 37.0 6 34.985183596427156 37.0 35.0 37.0 32.0 37.0 7 34.852648383699886 37.0 35.0 37.0 32.0 37.0 8 34.802763217361175 37.0 35.0 37.0 32.0 37.0 9 36.24256329819758 39.0 35.0 39.0 32.0 39.0 10-11 36.13935358809569 39.0 35.0 39.0 31.0 39.0 12-13 36.03251367437527 39.0 35.0 39.0 30.0 39.0 14-15 37.017554658074154 39.0 36.0 41.0 31.0 41.0 16-17 36.894773435011174 39.0 36.0 41.0 31.0 41.0 18-19 36.867883613594756 39.0 36.0 41.0 30.5 41.0 20-21 36.780051746116 39.0 36.0 41.0 30.0 41.0 22-23 36.637465602846305 39.0 36.0 40.5 30.0 41.0 24-25 36.7619439849646 39.0 36.0 41.0 30.0 41.0 26-27 36.71834594468429 39.0 36.0 41.0 30.0 41.0 28-29 36.618941268338574 39.0 36.0 41.0 30.0 41.0 30-31 36.42550276055886 39.0 35.5 41.0 30.0 41.0 32-33 36.21397564183613 39.0 35.0 40.0 30.0 41.0 34-35 35.96543522565929 38.0 35.0 40.0 29.0 41.0 36-37 35.75268452126957 38.0 35.0 40.0 27.5 41.0 38-39 35.53168593478025 38.0 35.0 40.0 27.0 41.0 40-41 35.35125988094428 38.0 34.5 40.0 26.5 41.0 42-43 35.09911584614407 38.0 34.0 40.0 25.5 41.0 44-45 34.806126669841845 38.0 34.0 40.0 24.5 41.0 46-47 34.488604499532954 38.0 33.0 40.0 24.0 41.0 48-49 34.34036611593672 38.0 33.0 40.0 23.0 41.0 50-51 33.404576314030976 37.5 32.0 39.5 22.0 40.5 52-53 33.55659945469218 37.5 33.0 39.5 22.0 40.5 54-55 33.98434616907967 38.0 33.0 40.0 22.0 41.0 56-57 33.87150908598534 38.0 33.0 40.0 21.5 41.0 58-59 33.60692304815444 38.0 33.0 40.0 20.5 41.0 60-61 33.26268385889766 37.0 32.5 40.0 19.0 41.0 62-63 32.948904991085016 37.0 32.0 40.0 18.0 41.0 64-65 32.611146908232655 36.0 31.5 39.0 16.5 41.0 66-67 32.201178796709044 36.0 31.0 39.0 13.0 41.0 68-69 31.775050387577686 35.0 31.0 39.0 10.0 40.0 70-71 31.319150613910622 35.0 30.5 38.0 8.0 40.0 72-73 30.908149968210655 35.0 30.0 37.0 7.0 39.5 74-75 30.48496595994137 35.0 30.0 37.0 4.5 39.0 76-77 30.058203340967886 34.0 29.0 36.0 2.0 39.0 78-79 29.601045511258747 34.0 29.0 36.0 2.0 38.5 80-81 29.098179964195868 34.0 29.0 35.5 2.0 37.0 82-83 28.598115979970235 34.0 27.5 35.0 2.0 37.0 84-85 28.26279335303026 34.0 27.0 35.0 2.0 36.0 86-87 27.953149127854452 34.0 27.0 35.0 2.0 36.0 88-89 27.52983760172703 33.5 26.0 35.0 2.0 36.0 90-91 27.087827361547372 33.0 25.5 35.0 2.0 35.0 92-93 26.693348839745198 33.0 25.0 35.0 2.0 35.0 94-95 26.210583576691178 33.0 24.5 35.0 2.0 35.0 96-97 25.5980643870559 32.5 21.5 35.0 2.0 35.0 98-99 24.895645558030314 32.0 10.5 35.0 2.0 35.0 100-101 22.466285494911226 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.377945728656357 1101 2 0.435611404084316 1101 3 0.5130433600347288 1101 4 0.5982407157871705 1101 5 0.5195211219805458 1101 6 0.34892131782821423 1101 7 0.5981222927664263 1101 8 0.40804729790669114 1101 9 0.667527894986371 1101 10-11 0.664906108198899 1101 12-13 0.7593503373767874 1101 14-15 0.8468758307559767 1101 16-17 0.9225949549741017 1101 18-19 0.9391953358150502 1101 20-21 0.9336467510293645 1101 22-23 0.8251231321112584 1101 24-25 0.8148176440567951 1101 26-27 0.8268840012135499 1101 28-29 0.7768655954264787 1101 30-31 0.8342125718446312 1101 32-33 0.7028023762878632 1101 34-35 0.555983270710577 1101 36-37 0.7776323034842747 1101 38-39 0.5211808079906817 1101 40-41 0.4946350712060479 1101 42-43 0.5005832418702454 1101 44-45 0.46610744897441947 1101 46-47 0.6709661116014445 1101 48-49 0.7118671319161223 1101 50-51 0.6751903537427921 1101 52-53 0.7034226277186661 1101 54-55 0.8211988037221118 1101 56-57 0.611898454261727 1101 58-59 0.6929011985742264 1101 60-61 0.6312172981787185 1101 62-63 0.827602609601449 1101 64-65 0.851607382487579 1101 66-67 0.890898032560699 1101 68-69 0.902785924530999 1101 70-71 0.9398573035636169 1101 72-73 0.689659672695722 1101 74-75 0.7238366836056578 1101 76-77 0.7377527492961526 1101 78-79 0.6385005787178137 1101 80-81 0.7433937983543721 1101 82-83 0.8718765304138252 1101 84-85 0.9326658122683789 1101 86-87 0.7275828747055151 1101 88-89 0.7391307374417799 1101 90-91 0.6250783356269345 1101 92-93 0.7352784735608111 1101 94-95 0.785170450094963 1101 96-97 0.561091757759371 1101 98-99 0.5578480442010196 1101 100-101 0.7955854888565206 1102 1 0.3477846189781779 1102 2 0.45276014188251423 1102 3 0.3774245669638141 1102 4 0.40727909510032134 1102 5 0.5099970074605977 1102 6 0.46167731984316873 1102 7 0.5086627121328249 1102 8 0.38501936380465196 1102 9 0.5509169972763885 1102 10-11 0.5931126789533465 1102 12-13 0.43227752383435813 1102 14-15 0.43801929921001914 1102 16-17 0.3557721756200962 1102 18-19 0.6339182884480792 1102 20-21 0.6767188790723537 1102 22-23 0.4001032682071326 1102 24-25 0.24477604670462227 1102 26-27 0.30654613308217193 1102 28-29 0.47416765313652576 1102 30-31 0.39300202484724167 1102 32-33 0.15931600578234395 1102 34-35 0.18095804052298803 1102 36-37 0.2965891325993084 1102 38-39 0.2057902204518811 1102 40-41 0.3065207832166479 1102 42-43 0.5506279897501329 1102 44-45 0.33359703232578397 1102 46-47 0.5223695678342111 1102 48-49 0.6022941149742564 1102 50-51 0.7189960181146517 1102 52-53 0.6469974553484477 1102 54-55 0.7129384489588446 1102 56-57 0.7173513772417124 1102 58-59 0.9287369799137579 1102 60-61 0.729100732115441 1102 62-63 0.8011031228604963 1102 64-65 0.8246714957265553 1102 66-67 0.7960311423565969 1102 68-69 0.5331763995509498 1102 70-71 0.6152681271842688 1102 72-73 0.6827319738446285 1102 74-75 0.5138560887791002 1102 76-77 0.7573725740783424 1102 78-79 0.5916923056397643 1102 80-81 0.7357558459204192 1102 82-83 0.8790206982157756 1102 84-85 0.7935734758404891 1102 86-87 0.7488117189984713 1102 88-89 0.8833318622840416 1102 90-91 0.9803750305156385 1102 92-93 0.7054756065805918 1102 94-95 0.8399141581275984 1102 96-97 0.6854946721706128 1102 98-99 0.8191226057670065 1102 100-101 0.7041002227667583 1103 1 0.3790925002860561 1103 2 0.502328877814886 1103 3 0.3060271682936886 1103 4 0.5637051015263239 1103 5 0.6304992325082779 1103 6 0.4634126852148981 1103 7 0.40436123964953197 1103 8 0.46040552555444947 1103 9 0.5779844016165185 1103 10-11 0.6731791726562051 1103 12-13 0.5683748417498649 1103 14-15 0.45398337199227257 1103 16-17 0.7439781342805958 1103 18-19 0.6329359170111672 1103 20-21 0.7462627872526255 1103 22-23 0.8273807204845838 1103 24-25 0.867214235051911 1103 26-27 0.6808197709921799 1103 28-29 0.3818526585488158 1103 30-31 0.4545976159882841 1103 32-33 0.6819311670338664 1103 34-35 0.5852749766581056 1103 36-37 0.5530649663478684 1103 38-39 0.5637071806415648 1103 40-41 0.44732549447590486 1103 42-43 0.691965767451542 1103 44-45 0.6251465761480546 1103 46-47 0.873549530377808 1103 48-49 0.7475536465974173 1103 50-51 0.8870150384064033 1103 52-53 0.9818460833788905 1103 54-55 1.1595471842039373 1103 56-57 1.0882148503779092 1103 58-59 0.9069963740822402 1103 60-61 0.8311999228509919 1103 62-63 0.8611201328775095 1103 64-65 1.0197128180405954 1103 66-67 0.8634479393188457 1103 68-69 0.9094117735135931 1103 70-71 1.0756747353181488 1103 72-73 1.0347147576455917 1103 74-75 0.8620515665200337 1103 76-77 0.9791222003734212 1103 78-79 0.916623148752425 1103 80-81 0.8220236935519019 1103 82-83 0.7791452665221605 1103 84-85 0.9749788640640631 1103 86-87 0.9756540412953392 1103 88-89 1.1409167835053218 1103 90-91 1.3597606939922073 1103 92-93 1.202817576649835 1103 94-95 0.9998199157606287 1103 96-97 1.1871802102197861 1103 98-99 1.1955306571750555 1103 100-101 1.3971836749411217 1104 1 0.17386357399811914 1104 2 0.3336813902249425 1104 3 0.3069331191077751 1104 4 0.3822727505305181 1104 5 0.38826328443342106 1104 6 0.279914218516609 1104 7 0.29391574702207635 1104 8 0.38695903745006177 1104 9 0.3644636202005387 1104 10-11 0.5248831752751784 1104 12-13 0.3197210739699159 1104 14-15 0.4708859355123849 1104 16-17 0.3589284940553199 1104 18-19 0.5666847561781694 1104 20-21 0.659040134473635 1104 22-23 0.2862603412680045 1104 24-25 0.3769433517498655 1104 26-27 0.1082495030151378 1104 28-29 0.4192563678695507 1104 30-31 0.46712575992489747 1104 32-33 0.1502424306214607 1104 34-35 0.0952087006103497 1104 36-37 0.061863579361222776 1104 38-39 0.13563322806368916 1104 40-41 0.2030195890929818 1104 42-43 0.2795172950743634 1104 44-45 0.2599630476166155 1104 46-47 0.23139759654695524 1104 48-49 0.17384993794478731 1104 50-51 0.2192335823376652 1104 52-53 0.30068982690662693 1104 54-55 0.40313587852570976 1104 56-57 0.37088904213750595 1104 58-59 0.378002774531204 1104 60-61 0.4189423615690302 1104 62-63 0.6155773975207026 1104 64-65 0.28279599047190374 1104 66-67 0.4575039733120434 1104 68-69 0.3610067079390582 1104 70-71 0.3617298740502193 1104 72-73 0.36897787088276246 1104 74-75 0.1953080120661106 1104 76-77 0.39961866943680846 1104 78-79 0.3320036013269707 1104 80-81 0.3727945200649927 1104 82-83 0.25338880427345245 1104 84-85 0.42259723562415275 1104 86-87 0.5880634295112976 1104 88-89 0.5573558489595989 1104 90-91 0.7644175550997936 1104 92-93 0.7467087556552912 1104 94-95 0.7856986746881844 1104 96-97 0.7490611271275291 1104 98-99 1.1561777505828594 1104 100-101 1.4570529267299719 1105 1 0.3263648554380687 1105 2 0.22234237564812886 1105 3 0.3044622315372969 1105 4 0.3046439620926975 1105 5 0.36586778424828026 1105 6 0.40531449270670095 1105 7 0.4619288218459232 1105 8 0.3985413022549551 1105 9 0.3480549015776191 1105 10-11 0.3947203951742395 1105 12-13 0.3912217005435821 1105 14-15 0.2916434261865817 1105 16-17 0.3867019056902308 1105 18-19 0.28796825768430523 1105 20-21 0.3071120512993346 1105 22-23 0.2385378298508698 1105 24-25 0.417839651573388 1105 26-27 0.41988229742120353 1105 28-29 0.2621254399481856 1105 30-31 0.2851518559655517 1105 32-33 0.20533020222633525 1105 34-35 0.23706352413563536 1105 36-37 0.28530567432444087 1105 38-39 0.1942482272479964 1105 40-41 0.06729036289740975 1105 42-43 0.2212357680942958 1105 44-45 -0.09504545505595274 1105 46-47 0.04033953250904432 1105 48-49 0.3093637432473173 1105 50-51 0.3270470238733907 1105 52-53 0.05909132446237919 1105 54-55 0.23898945982360686 1105 56-57 0.5316945342782802 1105 58-59 0.39730660435730414 1105 60-61 0.26408617569687465 1105 62-63 0.22629521567436228 1105 64-65 0.3915155399344954 1105 66-67 0.5803713974704863 1105 68-69 0.6526362164319686 1105 70-71 0.5742391105105575 1105 72-73 0.47815663746413506 1105 74-75 0.4651879521453459 1105 76-77 0.2984858401199766 1105 78-79 0.39276040040413207 1105 80-81 0.22420423637453624 1105 82-83 0.17047806761010875 1105 84-85 0.4197445633296297 1105 86-87 0.39558262794703225 1105 88-89 0.2119035461565808 1105 90-91 0.3788688390380983 1105 92-93 0.5117538864978393 1105 94-95 0.5019808376727859 1105 96-97 0.47195945806233297 1105 98-99 0.6067603300809452 1105 100-101 1.0712159073609548 1106 1 0.2164631239082233 1106 2 0.23866992532620301 1106 3 0.3398608531978802 1106 4 0.2706419172794199 1106 5 0.35250230323346443 1106 6 0.3655706848955944 1106 7 0.4563687602087896 1106 8 0.40806817378317817 1106 9 0.6390410503819481 1106 10-11 0.43849713198268603 1106 12-13 0.6470180160101222 1106 14-15 0.8108025728564243 1106 16-17 0.8682077355279176 1106 18-19 0.7379444430291144 1106 20-21 0.6487094317040842 1106 22-23 0.5630521698736715 1106 24-25 0.3904511156842503 1106 26-27 0.15771154491343253 1106 28-29 0.5094118226593665 1106 30-31 0.6279579710239034 1106 32-33 0.45507990018988664 1106 34-35 0.35855813896155553 1106 36-37 0.273703874202333 1106 38-39 0.276976772649526 1106 40-41 0.23448152400106892 1106 42-43 0.03388728053099754 1106 44-45 0.3544737850151165 1106 46-47 0.405502990548392 1106 48-49 0.4852996188182317 1106 50-51 0.2545068775466035 1106 52-53 0.5742571631260347 1106 54-55 0.6471543114114482 1106 56-57 0.42105623926963176 1106 58-59 0.5727116764161977 1106 60-61 0.7461918723878327 1106 62-63 0.6426225802862433 1106 64-65 0.6564840727919048 1106 66-67 0.7334379696524493 1106 68-69 0.5446905505225565 1106 70-71 0.7361655588091942 1106 72-73 0.8844131200387189 1106 74-75 0.665184368590733 1106 76-77 0.5890003224755098 1106 78-79 0.759367711292974 1106 80-81 0.7179180422626388 1106 82-83 0.8835402419461715 1106 84-85 0.961501386678389 1106 86-87 1.0483414013888215 1106 88-89 1.0185409117964177 1106 90-91 1.1387302177240919 1106 92-93 1.2302842623386923 1106 94-95 0.96730733903496 1106 96-97 1.1374528050165367 1106 98-99 1.4819232514283165 1106 100-101 2.012850317830047 1107 1 0.10147083652719502 1107 2 0.12506391218546753 1107 3 0.0010425059757928068 1107 4 0.06831737369678592 1107 5 0.2846554237686618 1107 6 0.2252483476036602 1107 7 0.13180084225339783 1107 8 0.29698665666209934 1107 9 0.33254588564272325 1107 10-11 0.3078664627965537 1107 12-13 0.36610472981346476 1107 14-15 0.1931262672202081 1107 16-17 0.3455821791091509 1107 18-19 0.4690726940394043 1107 20-21 0.48511782675263504 1107 22-23 0.6475512910328618 1107 24-25 0.5772850516105237 1107 26-27 0.2792603556126494 1107 28-29 0.286466533667344 1107 30-31 0.37644284544608553 1107 32-33 0.22791713195798025 1107 34-35 0.3851003396836674 1107 36-37 0.31026945865202293 1107 38-39 0.3906275124349463 1107 40-41 0.2550136552701616 1107 42-43 0.5374429229105075 1107 44-45 0.5866580211973087 1107 46-47 0.5358619432702341 1107 48-49 0.6018785692340884 1107 50-51 0.6013606643829377 1107 52-53 0.6840243210809049 1107 54-55 0.6884631019845173 1107 56-57 0.6949080022462795 1107 58-59 0.7328083094648647 1107 60-61 0.9161981005521298 1107 62-63 0.8306828486427591 1107 64-65 0.8350430759633767 1107 66-67 0.820899538723566 1107 68-69 0.8058330653885015 1107 70-71 0.8863745824475195 1107 72-73 0.792140625515426 1107 74-75 0.6585506435594048 1107 76-77 0.7068186121319755 1107 78-79 0.9282143193769663 1107 80-81 0.8756442470583075 1107 82-83 0.9281635873749998 1107 84-85 1.081052603050118 1107 86-87 0.998523723304185 1107 88-89 0.855542081738033 1107 90-91 0.8202548575739037 1107 92-93 0.8904755514008684 1107 94-95 0.798026723059273 1107 96-97 1.0029569313641886 1107 98-99 1.10591801379557 1107 100-101 1.4241363187930567 1108 1 -0.08204282986539368 1108 2 0.18883258981936635 1108 3 0.197930454604883 1108 4 0.19098346598947558 1108 5 0.24125277112644739 1108 6 0.04480517374406645 1108 7 -0.1288385662944549 1108 8 -0.032699165612925185 1108 9 0.14628818810025024 1108 10-11 -0.1559943127332275 1108 12-13 0.17131506361601367 1108 14-15 0.2143693828771731 1108 16-17 0.2745931175910954 1108 18-19 0.10541387218012943 1108 20-21 0.08005765843427781 1108 22-23 0.3668156860977234 1108 24-25 0.21530672938909134 1108 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-2.143308196982744 2316 68-69 -2.8200691536946074 2316 70-71 -2.8403336284174863 2316 72-73 -2.3202017290890744 2316 74-75 -1.854450679527666 2316 76-77 -1.9985525818468126 2316 78-79 -2.72276830882085 2316 80-81 -2.418737108572536 2316 82-83 -2.168449849254774 2316 84-85 -2.224375242108227 2316 86-87 -2.849455779269025 2316 88-89 -3.00618072722855 2316 90-91 -3.030689880549275 2316 92-93 -3.3924222913173097 2316 94-95 -3.712407394193953 2316 96-97 -4.451580864904926 2316 98-99 -5.562720776076379 2316 100-101 -6.042099823433286 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5891.0 3 2403.0 4 2492.0 5 2751.0 6 3115.0 7 3697.0 8 4492.0 9 5737.0 10 7259.0 11 9120.0 12 11255.0 13 13558.0 14 15384.0 15 17673.0 16 21331.0 17 25266.0 18 21581.0 19 16583.0 20 16885.0 21 18028.0 22 19847.0 23 22502.0 24 25498.0 25 29199.0 26 33885.0 27 39768.0 28 46857.0 29 55782.0 30 66111.0 31 80321.0 32 99174.0 33 123928.0 34 159995.0 35 213506.0 36 288439.0 37 365342.0 38 280925.0 39 43700.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.917494560712456 18.48474317593092 14.479164835541274 22.11859742781535 2 15.344379385190047 20.438230564549276 40.309586304866706 23.907803745393974 3 20.373338212746624 24.60360024985961 29.55198332827064 25.47107820912312 4 12.544095298590257 16.57699213824499 38.04653938969627 32.83237317346848 5 13.378473906590663 37.721156949977185 34.07394951012304 14.82641963330911 6 30.89121122752858 36.244050522436034 17.786762789968 15.077975460067385 7 28.08851393477764 32.997389356454505 20.815543224385962 18.098553484381895 8 26.074493659217364 33.50217745356261 19.857718364040664 20.565610523179366 9 25.977076054181904 17.63693219238011 21.14480888973435 35.24118286370363 10-11 24.954783565200927 26.379146342562954 27.12445113649683 21.541618955739285 12-13 26.70295299553795 24.032439067434286 27.343805114961427 21.92080282206634 14-15 23.125695154232677 25.528801782299386 26.922998040327116 24.42250502314082 16-17 21.662335999653788 28.62423634803055 27.388170517083445 22.325257135232217 18-19 21.37670060609503 27.645993977594163 29.683892442419396 21.293412973891414 20-21 22.824560901479384 26.09567053849518 29.362994794876307 21.716773765149128 22-23 22.016025158570564 26.11238251987709 29.59721680158422 22.274375519968128 24-25 22.40337566964653 26.19111295664221 29.712692715241634 21.692818658469626 26-27 22.01586246521636 25.97888759956288 30.357175850297985 21.648074084922772 28-29 21.3229946331286 27.250773938003846 29.830274381863493 21.59595704700406 30-31 21.80824373446304 26.263443773577933 29.59019261607221 22.338119875886818 32-33 21.74462882033103 26.38712056915275 29.751870976783145 22.116379633733075 34-35 22.718148531098638 26.23507604012303 29.43458107014726 21.612194358631072 36-37 22.415082844247046 25.96219014521176 29.92788981498839 21.6948371955528 38-39 21.868341835355192 26.694035366007533 29.806586513329847 21.631036285307424 40-41 22.11584821287759 26.708965937386502 29.456225616938212 21.71896023279769 42-43 21.71975265640229 26.83830338117058 28.791250781228527 22.650693181198605 44-45 22.17342707948679 26.379770152648042 28.87299366837212 22.57380909949305 46-47 22.830350468416206 27.007863336287734 28.412458595936442 21.749327599359614 48-49 22.512109250117547 26.93515380814766 28.394932668311196 22.15780427342359 50-51 21.883683096016497 27.391389275085885 28.752725251606314 21.9722023772913 52-53 22.436545615780595 27.701384983633975 27.720603343859494 22.141466056725932 54-55 21.977252705409466 27.123602796547353 28.126885562813044 22.772258935230145 56-57 22.565083614150243 27.193915201743152 27.75194374873956 22.489057435367044 58-59 23.119771635658022 26.893555926529196 27.720923776934235 22.265748660878547 60-61 22.361381054460686 27.196635373016022 28.69547250738705 21.746511065136247 62-63 22.004066803728843 27.336175965302616 28.555631283972176 22.104125946996366 64-65 21.854317765633787 27.94859270687641 27.978196689400605 22.218892838089197 66-67 22.148470742029264 27.415227667890324 27.500439917815918 22.935861672264494 68-69 22.02895931639447 27.31019601471964 27.979907527939574 22.680937140946323 70-71 22.657256850753342 27.131403063845433 27.653714989194167 22.557625096207055 72-73 22.45599339053158 26.849906533338768 28.390456104239263 22.303643971890384 74-75 21.89346299453479 28.214153669792463 27.60391660221082 22.288466733461924 76-77 22.201571102351473 27.335220698986205 27.59822769380515 22.86498050485717 78-79 22.317325132087376 27.481451440287497 27.7202113397997 22.48101208782543 80-81 22.714031201577768 27.393749635266584 27.396611173111097 22.495607990044554 82-83 22.509406087714147 27.291161645918336 27.937190157871765 22.26224210849575 84-85 22.285940775394955 27.718103913117837 27.634370974323772 22.361584337163432 86-87 22.20730952819761 27.841333842222443 27.450249255028698 22.50110737455125 88-89 22.14665359874728 27.74630211677763 27.009834735881576 23.097209548593508 90-91 22.36118521135392 27.271320141887944 27.322622043099905 23.044872603658227 92-93 22.723690991205565 27.50647319160174 27.535653534911297 22.234182282281402 94-95 22.29982224547519 27.344116672220792 27.68759366248451 22.668467419819503 96-97 21.77665420674458 27.409778222399474 28.216000522730912 22.597567048125043 98-99 21.97912615211553 27.775296515812446 27.248740678179225 22.9968366538928 100-101 21.98038372072844 27.19824459114421 27.496011407633354 23.325360280494 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 320.0 1 239.5 2 310.5 3 459.0 4 383.0 5 354.0 6 581.5 7 1133.0 8 1876.0 9 2293.5 10 2162.5 11 2000.0 12 2100.0 13 2257.5 14 2485.5 15 2703.0 16 2819.5 17 3042.0 18 3435.5 19 3796.5 20 4127.5 21 4851.0 22 5791.0 23 7022.0 24 8581.0 25 10611.5 26 13733.5 27 17313.5 28 20310.5 29 23737.0 30 28017.5 31 32469.0 32 37418.5 33 41530.5 34 45521.0 35 49849.0 36 54810.0 37 58837.0 38 60321.0 39 61662.5 40 63096.5 41 64766.5 42 66048.5 43 68131.5 44 71360.0 45 74765.5 46 77638.5 47 87488.5 48 109152.5 49 108295.5 50 87015.0 51 76380.0 52 72228.0 53 70364.5 54 68296.5 55 63593.5 56 57629.0 57 52000.5 58 48015.5 59 43305.5 60 36277.5 61 30225.5 62 25127.0 63 19371.0 64 13685.5 65 10033.0 66 7697.5 67 6004.0 68 4667.5 69 3636.0 70 2855.0 71 2142.0 72 1722.0 73 1441.5 74 1095.0 75 822.5 76 621.0 77 393.0 78 249.0 79 172.5 80 116.0 81 73.5 82 52.5 83 39.0 84 25.0 85 17.5 86 14.5 87 11.5 88 6.0 89 4.0 90 3.5 91 3.0 92 3.5 93 4.0 94 4.0 95 1.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03226246870067413 2 0.020952580929907084 3 0.020321750536318486 4 0.04911465207225531 5 0.05357552414120327 6 0.046636389811728664 7 0.04821346579570017 8 0.03965219616842631 9 0.04136445009388108 10-11 0.04852888099249447 12-13 0.04156721700610599 14-15 0.03670081111270821 16-17 0.045464847652206986 18-19 0.04868658859089162 20-21 0.0105213497787813 22-23 0.018519377983208195 24-25 0.0055197659439002525 26-27 0.010003167669762092 28-29 0.005722532856125161 30-31 0.008718977225671012 32-33 0.003086562997201366 34-35 0.007772731635288112 36-37 0.008696447568757135 38-39 0.009777871100623305 40-41 0.0013968387286604723 42-43 0.0016221352977992582 44-45 0.0018699615238519224 46-47 4.505931382775717E-4 48-49 0.0010138345611245363 50-51 2.928855398804216E-4 52-53 0.002928855398804216 54-55 0.005947829425263946 56-57 0.014621747337107202 58-59 0.009530044874570641 60-61 0.009462455903829006 62-63 0.00493399486413941 64-65 4.5059313827757174E-5 66-67 0.005970359082177825 68-69 0.016063645379595433 70-71 0.0056324142284696465 72-73 0.0025458512312682797 74-75 0.0034920968216511803 76-77 0.006961663986388482 78-79 0.005497236286986375 80-81 0.009484985560742884 82-83 0.0055197659439002525 84-85 0.015207518416868043 86-87 0.003784982361531602 88-89 0.005497236286986375 90-91 0.0036498044200483307 92-93 0.015207518416868043 94-95 0.010160875268159243 96-97 0.008200795116651805 98-99 0.020006335339524183 100-101 0.03525891307021998 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2219297.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.86264669799397 #Duplication Level Percentage of deduplicated Percentage of total 1 81.11509245266139 49.368792138214474 2 11.549157601324039 14.058245974976732 3 3.3047729696279844 6.034116890026453 4 1.3292464434542406 3.2360582665008195 5 0.7016160516122923 2.1351104933460223 6 0.4200682184513257 1.5339878141195271 7 0.2879696349002227 1.22686159040778 8 0.19825643469249676 0.9653129072234676 9 0.14691943780792527 0.8047715252734489 >10 0.8453071007510587 9.501415952149776 >50 0.06590055959242727 2.7568122224794416 >100 0.031985703085428356 3.5201327014051613 >500 0.0016312524972205537 0.630081860319102 >1k 0.0016312524972203705 1.9853309899022877 >5k 3.707392039137206E-4 1.6153237436083576 >10k+ 7.41478407827441E-5 0.627644930047076 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13908 0.6266849367164466 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9035 0.40711090043378595 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8959 0.40368639258287653 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7080 0.31901994190052074 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5499 0.24778116673883666 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5221 0.2352546774947202 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4131 0.18614002542246486 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4015 0.18091314501844502 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3247 0.14630759199872753 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3148 0.14184671992977957 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3048 0.13734078854700385 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2757 0.12422852822312651 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2482 0.1118372169204933 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2471 0.11134156446838796 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2265 0.10205934581986999 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.505931382775717E-5 0.0 10-11 0.0 0.0 0.0 9.011862765551433E-5 0.0 12-13 0.0 0.0 0.0 9.011862765551433E-5 4.505931382775717E-5 14-15 0.0 2.2529656913878584E-5 0.0 1.1264828456939292E-4 4.505931382775717E-5 16-17 0.0 4.505931382775717E-5 0.0 1.351779414832715E-4 4.505931382775717E-5 18-19 0.0 4.505931382775717E-5 0.0 1.5770759839715007E-4 4.505931382775717E-5 20-21 0.0 4.505931382775717E-5 0.0 2.0276691222490727E-4 4.505931382775717E-5 22-23 0.0 4.505931382775717E-5 0.0 3.604745106220574E-4 4.505931382775717E-5 24-25 0.0 4.505931382775717E-5 0.0 4.505931382775717E-4 4.505931382775717E-5 26-27 0.0 4.505931382775717E-5 0.0 5.40711765933086E-4 6.758897074163575E-5 28-29 0.0 4.505931382775717E-5 0.0 7.434786781579933E-4 9.011862765551433E-5 30-31 0.0 4.505931382775717E-5 0.0 0.0014418980424882293 9.011862765551433E-5 32-33 0.0 4.505931382775717E-5 0.0 0.0026134402020099156 9.011862765551433E-5 34-35 0.0 4.505931382775717E-5 0.0 0.0035371561354789376 9.011862765551433E-5 36-37 0.0 4.505931382775717E-5 0.0 0.004708698295000624 9.011862765551433E-5 38-39 0.0 4.505931382775717E-5 0.0 0.005654943885383524 9.011862765551433E-5 40-41 0.0 4.505931382775717E-5 0.0 0.007029252957130118 9.011862765551433E-5 42-43 0.0 4.505931382775717E-5 0.0 0.009822930414451063 9.011862765551433E-5 44-45 0.0 4.505931382775717E-5 0.0 0.013788150031293692 9.011862765551433E-5 46-47 0.0 4.505931382775717E-5 0.0 0.023002779709070033 9.011862765551433E-5 48-49 0.0 4.505931382775717E-5 0.0 0.0424008143119195 1.351779414832715E-4 50-51 0.0 4.505931382775717E-5 0.0 0.05731544718890712 1.351779414832715E-4 52-53 0.0 4.505931382775717E-5 0.0 0.07259055457651681 1.351779414832715E-4 54-55 0.0 4.505931382775717E-5 0.0 0.09223641540541894 1.351779414832715E-4 56-57 0.0 4.505931382775717E-5 0.0 0.10931389534613889 1.351779414832715E-4 58-59 0.0 4.505931382775717E-5 0.0 0.13251944196743384 1.351779414832715E-4 60-61 0.0 4.505931382775717E-5 0.0 0.16962578690459185 1.351779414832715E-4 62-63 0.0 4.505931382775717E-5 0.0 0.19781038770385395 1.351779414832715E-4 64-65 0.0 4.505931382775717E-5 0.0 0.23099657233799714 1.351779414832715E-4 66-67 0.0 4.505931382775717E-5 0.0 0.29054245556137825 1.351779414832715E-4 68-69 0.0 4.505931382775717E-5 0.0 0.3600689767976075 1.351779414832715E-4 70-71 0.0 4.505931382775717E-5 0.0 0.4209441097789075 1.351779414832715E-4 72-73 0.0 4.505931382775717E-5 0.0 0.4837793229117149 1.351779414832715E-4 74-75 0.0 4.505931382775717E-5 0.0 0.5379856774465066 1.351779414832715E-4 76-77 0.0 4.505931382775717E-5 0.0 0.5832928175003165 1.351779414832715E-4 78-79 0.0 4.505931382775717E-5 0.0 0.6493948308856363 1.351779414832715E-4 80-81 0.0 4.505931382775717E-5 0.0 0.7441996271792375 1.351779414832715E-4 82-83 0.0 4.505931382775717E-5 0.0 0.851530912716955 1.351779414832715E-4 84-85 0.0 4.505931382775717E-5 0.0 0.9779448176607277 1.351779414832715E-4 86-87 0.0 4.505931382775717E-5 0.0 1.1813876195930513 1.351779414832715E-4 88-89 0.0 4.505931382775717E-5 0.0 1.4377525856160758 1.351779414832715E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1875 0.0 25.346235 2 GTACCTG 2050 0.0 24.572931 1 GTACATG 10190 0.0 24.064701 1 TACATGG 10215 0.0 23.77373 2 ACATGGG 10125 0.0 22.811613 3 GAGTACT 8975 0.0 22.053658 12-13 GTACACG 330 1.8189894E-12 21.601421 1 AGTACTT 9410 0.0 21.387688 12-13 GTACTTT 9575 0.0 20.57221 14-15 GTGTAGC 1445 0.0 20.390545 1 TAGCGCG 140 4.837057E-4 20.367512 4 GTATAGG 610 0.0 20.255758 1 CATGGGG 4695 0.0 19.94093 4 AGAGTAC 15715 0.0 19.8365 10-11 CGTATAG 130 0.0075440863 18.278124 1 TACTTTT 11090 0.0 18.018957 14-15 CATGGGA 5345 0.0 17.782673 4 ACTTTTT 11400 0.0 17.715157 16-17 ATGGGGA 2610 0.0 16.933146 5 TAGCCCT 1720 0.0 16.85451 4 >>END_MODULE