FastQCFastQC Report
Thu 26 May 2016
SRR936729_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936729_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219297
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT160540.7233822241908135No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97980.44149115688436474No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85620.38579784499325687No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT81420.3668729331855989No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52360.2359305672021365No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT48450.21831237549548346No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC46850.21110288528304236No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT42230.19028548229461853No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC38650.17415424794428144No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC36710.16541274106169657No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG30550.13765620374379817No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30370.1368451360948985No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA29120.13121272186642888No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA28050.12639137528685887No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26650.12008307135097285No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC23620.10643009926116244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG18100.024.9282552
GTACCTG18900.024.8861221
GGACCGT2354.660251E-922.232156
GAGTACT107200.021.97747412-13
GTACATG101700.021.2556461
TACATGG102700.020.3483452
GTACTTT114800.020.29472414-15
ACATGGG101200.019.8985943
AGTACTT112800.018.84407612-13
AGAGTAC176700.017.68865810-11
ACTTTTT131350.017.66448216-17
CTAATAC7200.017.1505453
ACACCGT4751.8189894E-1216.9985526
CATGGGA53800.016.772914
AGTACAT81100.016.3391762
TAGCCCT13450.016.2432384
TACTTTT128250.016.18490214-15
GAGTACA96100.016.0673351
CATGGGG45600.016.039584
TATACGG1800.002621521215.8316295
Produced by FastQC (version 0.11.5)