##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936729_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2219297 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78169708696042 31.0 31.0 34.0 30.0 34.0 2 31.91760814347967 31.0 31.0 34.0 30.0 34.0 3 32.02365118323505 31.0 31.0 34.0 30.0 34.0 4 35.78096307073817 37.0 35.0 37.0 35.0 37.0 5 35.553489235555226 37.0 35.0 37.0 33.0 37.0 6 35.65476995643215 37.0 35.0 37.0 33.0 37.0 7 35.60110161010446 37.0 35.0 37.0 33.0 37.0 8 35.58095514029893 37.0 35.0 37.0 33.0 37.0 9 37.18602647595162 39.0 37.0 39.0 33.0 39.0 10-11 37.05200633353715 39.0 37.0 39.0 33.0 39.0 12-13 36.956342932018565 39.0 37.0 39.0 33.0 39.0 14-15 38.00022281830688 40.0 37.0 41.0 33.0 41.0 16-17 37.94032231828368 40.0 37.0 41.0 33.0 41.0 18-19 38.119581335891496 40.0 37.0 41.0 33.0 41.0 20-21 38.15905577306688 40.0 37.0 41.0 33.0 41.0 22-23 38.06575618315169 40.0 37.0 41.0 33.0 41.0 24-25 37.934624568050154 40.0 37.0 41.0 33.0 41.0 26-27 37.70045897417065 39.5 37.0 41.0 32.0 41.0 28-29 37.61565779613995 39.0 37.0 41.0 32.0 41.0 30-31 37.576706047004976 39.0 37.0 41.0 32.0 41.0 32-33 37.40912910710013 39.0 36.0 41.0 32.0 41.0 34-35 37.21199348262085 39.0 36.0 41.0 31.0 41.0 36-37 36.932398187353925 39.0 35.5 41.0 30.5 41.0 38-39 36.755072439605875 39.0 35.0 41.0 30.0 41.0 40-41 36.55973851179 39.0 35.0 40.5 30.0 41.0 42-43 36.29314620801091 39.0 35.0 40.0 30.0 41.0 44-45 36.020794422738376 39.0 35.0 40.0 29.5 41.0 46-47 35.64116001598704 38.0 35.0 40.0 28.5 41.0 48-49 35.25246373063182 38.0 34.5 40.0 27.0 41.0 50-51 34.92148775040024 38.0 34.0 40.0 26.0 41.0 52-53 34.652492658711296 38.0 34.0 40.0 25.0 41.0 54-55 34.39378866370747 38.0 33.5 40.0 24.0 41.0 56-57 34.052739673869695 38.0 33.0 40.0 23.5 41.0 58-59 33.711849968706304 37.0 33.0 40.0 23.0 41.0 60-61 33.32170840586005 37.0 32.0 39.5 22.0 41.0 62-63 32.94162115300476 36.0 32.0 39.0 21.5 40.0 64-65 32.5270761416791 36.0 31.0 39.0 20.0 40.0 66-67 32.04123039863524 35.0 31.0 38.5 18.5 40.0 68-69 31.73542747996325 35.0 31.0 38.0 18.0 40.0 70-71 31.277574835634887 35.0 30.0 37.5 15.5 39.5 72-73 30.813567089037655 34.0 30.0 37.0 12.5 39.0 74-75 30.291680428532096 34.0 29.0 36.0 8.0 39.0 76-77 28.886831505652466 32.5 28.0 35.0 7.5 37.0 78-79 29.670673866544227 34.0 29.0 35.0 7.0 37.0 80-81 29.44655920320714 34.0 29.0 35.0 4.5 37.0 82-83 29.081340397432157 34.0 29.0 35.0 2.0 36.5 84-85 28.64422179636164 33.5 28.5 35.0 2.0 36.0 86-87 28.26561429137245 33.0 27.5 35.0 2.0 36.0 88-89 27.837467675574743 33.0 27.0 35.0 2.0 35.0 90-91 27.3164693594413 33.0 26.0 35.0 2.0 35.0 92-93 26.785306112701456 32.0 25.0 34.0 2.0 35.0 94-95 26.15686025800062 32.0 24.5 34.0 2.0 35.0 96-97 25.330205916558263 32.0 21.5 34.0 2.0 35.0 98-99 24.34453703132118 31.0 10.0 34.0 2.0 35.0 100-101 21.096036267340516 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2647351141102092 1101 2 0.323393812449261 1101 3 0.24667852252754585 1101 4 0.14393109781608615 1101 5 0.17219443127953582 1101 6 0.12487008683915235 1101 7 0.15451825147994214 1101 8 0.17975264105712796 1101 9 0.2863636556733198 1101 10-11 0.27853254647057923 1101 12-13 0.2156324717611966 1101 14-15 0.41767132461423984 1101 16-17 0.44631905631715796 1101 18-19 0.4085059050748825 1101 20-21 0.36134803051543685 1101 22-23 0.23613853193299406 1101 24-25 0.2767219037920867 1101 26-27 0.27604046074112887 1101 28-29 0.32002062215003235 1101 30-31 0.3852926776297352 1101 32-33 0.3744867882574283 1101 34-35 0.25227333351853787 1101 36-37 0.29779122492640653 1101 38-39 0.342299344061594 1101 40-41 0.38615501347153014 1101 42-43 0.5675549132457647 1101 44-45 0.42402960540017887 1101 46-47 0.43799902199581453 1101 48-49 0.38796534400511007 1101 50-51 0.5270490187913808 1101 52-53 0.49106337653316956 1101 54-55 0.4826824546326094 1101 56-57 0.45018537970991446 1101 58-59 0.423073203328201 1101 60-61 0.48285747536457535 1101 62-63 0.6000692432155077 1101 64-65 0.5202115869955222 1101 66-67 0.40202529254000297 1101 68-69 0.5214436139631289 1101 70-71 0.6579624692362849 1101 72-73 0.5884851727317866 1101 74-75 0.5594331920630751 1101 76-77 0.4371974984692457 1101 78-79 0.3919022998484145 1101 80-81 0.5473637097495612 1101 82-83 0.6709337902970915 1101 84-85 0.7781278325828147 1101 86-87 0.7218700510729015 1101 88-89 0.5298677656484152 1101 90-91 0.6347418708761303 1101 92-93 0.705211693103859 1101 94-95 0.7943823232476781 1101 96-97 0.7744977704863416 1101 98-99 1.008715404005418 1101 100-101 1.254298822680024 1102 1 0.20981778084150093 1102 2 0.1983753333924838 1102 3 0.2467645561689551 1102 4 0.18520283775560387 1102 5 0.0644451717400969 1102 6 0.15593636736421956 1102 7 0.25505384119122 1102 8 0.16177212932140606 1102 9 0.2924509252909786 1102 10-11 0.37523453251477434 1102 12-13 0.26242317458854103 1102 14-15 0.37186819105091473 1102 16-17 0.3787198969194989 1102 18-19 0.32826810947488383 1102 20-21 0.21499329494337616 1102 22-23 0.16927888101839272 1102 24-25 0.166932071687512 1102 26-27 0.12288344155579267 1102 28-29 0.12305014882652188 1102 30-31 0.282243763090392 1102 32-33 0.31366143654947365 1102 34-35 0.30952378835434047 1102 36-37 0.48547127671970003 1102 38-39 0.5503038482775864 1102 40-41 0.5440447484636834 1102 42-43 0.43386893430583484 1102 44-45 0.6295386146977009 1102 46-47 0.5610115496711288 1102 48-49 0.5845015811325212 1102 50-51 0.5992926965304761 1102 52-53 0.5229325223073928 1102 54-55 0.589945286541564 1102 56-57 0.5581221995118426 1102 58-59 0.3659753413594018 1102 60-61 0.5600633208052201 1102 62-63 0.5221012124165085 1102 64-65 0.6324706884719475 1102 66-67 0.6842660967208616 1102 68-69 0.5538175365951759 1102 70-71 0.600491996778306 1102 72-73 0.6535136226894664 1102 74-75 0.5012217397217285 1102 76-77 0.29982202653923906 1102 78-79 0.4392775813619174 1102 80-81 0.9684322579621529 1102 82-83 0.794170749795498 1102 84-85 0.6558205384187339 1102 86-87 0.7161262235971826 1102 88-89 0.7575429877865858 1102 90-91 0.9340388011888798 1102 92-93 1.0019476079080505 1102 94-95 0.8993021665522001 1102 96-97 1.188356197542106 1102 98-99 1.1546871427331915 1102 100-101 1.629984971263756 1103 1 0.23190159383440445 1103 2 0.25644478237102675 1103 3 0.19843119419922317 1103 4 0.17969605952155376 1103 5 0.053259796918361246 1103 6 0.13531902401960139 1103 7 0.1845713752542082 1103 8 0.20270355661646988 1103 9 0.23960303607945121 1103 10-11 0.12250347978372389 1103 12-13 0.2648844725043844 1103 14-15 0.28050635877998786 1103 16-17 0.3800149777600339 1103 18-19 0.236468140402188 1103 20-21 0.26469561964569976 1103 22-23 0.24490515891739761 1103 24-25 0.2646279875652411 1103 26-27 0.3859347636980246 1103 28-29 0.4556470973325659 1103 30-31 0.19335224919888105 1103 32-33 0.24154884398233634 1103 34-35 0.19715931008076382 1103 36-37 0.3007759965698753 1103 38-39 0.41482206961399015 1103 40-41 0.3166710779081967 1103 42-43 0.3272290140295482 1103 44-45 0.37278787158331994 1103 46-47 0.42315223453908146 1103 48-49 0.4140057992731059 1103 50-51 0.4171218575414528 1103 52-53 0.2763409688976566 1103 54-55 0.35553479531289156 1103 56-57 0.34523840026440666 1103 58-59 0.5737252082001731 1103 60-61 0.5816510083019963 1103 62-63 0.4915656546082161 1103 64-65 0.2995086259644282 1103 66-67 0.6475768645770827 1103 68-69 0.6208881195748432 1103 70-71 0.4720951479269111 1103 72-73 0.2985515086364394 1103 74-75 0.19270126756489603 1103 76-77 0.3837561513824532 1103 78-79 0.29050780077395544 1103 80-81 0.4771236893808606 1103 82-83 0.5127971479673548 1103 84-85 0.4767252479598092 1103 86-87 0.49464549302554417 1103 88-89 0.4841460530260129 1103 90-91 0.6266239801376976 1103 92-93 0.7493849371635584 1103 94-95 0.7615283310510925 1103 96-97 0.6827527714841324 1103 98-99 0.8774642427830202 1103 100-101 1.0122125103094781 1104 1 0.13990549556947585 1104 2 0.1758325306556614 1104 3 0.1338918862227203 1104 4 0.11208179680917851 1104 5 -1.1246468496040052E-4 1104 6 0.10471879820000396 1104 7 0.09789506560075267 1104 8 0.07513051600235343 1104 9 0.02700925079513894 1104 10-11 0.09305907616793974 1104 12-13 0.06355460886467057 1104 14-15 0.1582708115683218 1104 16-17 0.17213818306211692 1104 18-19 0.16100163586241223 1104 20-21 0.04603059429111056 1104 22-23 0.23022137316540636 1104 24-25 0.10955573197487212 1104 26-27 0.08698502526126362 1104 28-29 0.1283732008755223 1104 30-31 0.024951793551053925 1104 32-33 0.1344547346669458 1104 34-35 0.13343592572645946 1104 36-37 0.20518515763001943 1104 38-39 0.0840212451720248 1104 40-41 0.08275788483243929 1104 42-43 0.05225390095272786 1104 44-45 -0.035153896356973746 1104 46-47 0.0486631680826477 1104 48-49 7.671253285437274E-4 1104 50-51 -0.24865628472489476 1104 52-53 0.04077521731258571 1104 54-55 0.004796705960487202 1104 56-57 -0.14212939839537597 1104 58-59 -0.13359154721314326 1104 60-61 -0.11280889535721172 1104 62-63 -0.22847349636354153 1104 64-65 0.02853629888678455 1104 66-67 -0.3372878775008594 1104 68-69 -0.16423835276225418 1104 70-71 -0.24854346054332765 1104 72-73 -0.30381792688137566 1104 74-75 -0.09596900241646367 1104 76-77 -0.17064900104577418 1104 78-79 -0.01897027534996809 1104 80-81 0.24787971736111203 1104 82-83 0.007529304544515725 1104 84-85 -0.018993032069854365 1104 86-87 -0.022484727382401815 1104 88-89 0.10865265724710227 1104 90-91 0.09691600897312114 1104 92-93 0.22586529152662038 1104 94-95 0.3564168242731647 1104 96-97 0.7295941365569227 1104 98-99 0.9345394781231207 1104 100-101 1.2363539949731184 1105 1 0.06005184941367858 1105 2 0.09001311557467773 1105 3 0.06950337546937391 1105 4 0.07579082175332985 1105 5 0.006739971341488626 1105 6 0.027761421424230548 1105 7 0.09056843888346577 1105 8 -0.00471568473087558 1105 9 -0.11584193572394241 1105 10-11 -0.027948646886166273 1105 12-13 -0.0741345834591911 1105 14-15 0.0847585145429548 1105 16-17 0.05122898776669871 1105 18-19 0.019796963181391902 1105 20-21 0.11072602190819936 1105 22-23 0.058120264988708925 1105 24-25 0.12713445739132823 1105 26-27 0.22288888797958606 1105 28-29 0.11879623654969151 1105 30-31 0.2153324895888602 1105 32-33 0.314630899983122 1105 34-35 0.2007221122174201 1105 36-37 -0.013343785705679068 1105 38-39 0.08896111759388248 1105 40-41 0.08962306080417193 1105 42-43 -0.0664478092773848 1105 44-45 -0.010720584014222823 1105 46-47 -0.0958186487063486 1105 48-49 -0.09464406219650101 1105 50-51 0.05769274146346959 1105 52-53 -0.16442071146417447 1105 54-55 -0.07160168503405373 1105 56-57 -0.16802854898848096 1105 58-59 -0.020389026093795337 1105 60-61 -0.06941672443414149 1105 62-63 -0.023622621559475476 1105 64-65 -0.23510172981681166 1105 66-67 -0.04621396298556135 1105 68-69 0.08703655176261194 1105 70-71 -0.05166721240096095 1105 72-73 0.04320345736497089 1105 74-75 -0.2181545085943455 1105 76-77 -0.2693666180075738 1105 78-79 -0.15326898768894992 1105 80-81 0.006357323680916238 1105 82-83 -0.06398497197053743 1105 84-85 -0.11921296985259744 1105 86-87 0.02254270651796375 1105 88-89 0.155384927462336 1105 90-91 0.33131354688769576 1105 92-93 0.18333215691437843 1105 94-95 0.2053689720459424 1105 96-97 0.41210320094526764 1105 98-99 0.6561493373185989 1105 100-101 0.9250632763707145 1106 1 0.11233591122646303 1106 2 0.15778856432235955 1106 3 0.1726155328214105 1106 4 0.08872005126048776 1106 5 0.06365270707586745 1106 6 -3.3246905258010884E-4 1106 7 0.1229291147385041 1106 8 0.00664165976430553 1106 9 -0.007123618426597034 1106 10-11 0.08448083658228711 1106 12-13 0.023137725808638265 1106 14-15 0.10552546589190825 1106 16-17 0.12547300495497637 1106 18-19 -0.009993731992871346 1106 20-21 0.07370899632245909 1106 22-23 0.0578646212687417 1106 24-25 -0.06981610587447307 1106 26-27 0.05887077100798166 1106 28-29 0.017084105005984895 1106 30-31 0.11185634066845296 1106 32-33 0.1693444267577462 1106 34-35 0.21402256769898287 1106 36-37 0.09330927691281943 1106 38-39 0.18361675902576025 1106 40-41 0.27736876773098373 1106 42-43 0.3930846899457592 1106 44-45 0.40844174242943154 1106 46-47 0.2603992139169904 1106 48-49 0.4104011789186828 1106 50-51 0.420819041238083 1106 52-53 0.2641150498031166 1106 54-55 0.3911603876333629 1106 56-57 0.5387221108145894 1106 58-59 0.3915029746552179 1106 60-61 0.363263257913907 1106 62-63 0.4578022838758997 1106 64-65 0.5413072590840997 1106 66-67 0.3302980909649378 1106 68-69 0.45378198933397584 1106 70-71 0.5132561979518755 1106 72-73 0.2909370273138556 1106 74-75 0.44175202624462173 1106 76-77 0.1886664243848699 1106 78-79 0.31210563088540155 1106 80-81 0.3203827002948394 1106 82-83 0.6952428073410459 1106 84-85 0.4966344339798532 1106 86-87 0.4918753863993679 1106 88-89 0.40914248636511985 1106 90-91 0.6146819502660605 1106 92-93 0.6711174511839353 1106 94-95 0.7279758179138902 1106 96-97 0.7219857865354413 1106 98-99 1.1818792594444218 1106 100-101 1.2448154424444091 1107 1 0.021291427431521015 1107 2 0.06839670067803993 1107 3 0.03254007318170693 1107 4 0.01859437861958213 1107 5 0.03643322915077363 1107 6 0.04695760816088068 1107 7 -0.010305433967971567 1107 8 -0.038762010371570454 1107 9 0.04409803994339967 1107 10-11 0.1016860775044961 1107 12-13 0.01725042386249953 1107 14-15 0.10565863884708193 1107 16-17 0.016610197937609428 1107 18-19 0.02873467523340878 1107 20-21 0.08338321012790573 1107 22-23 -0.03825654648436938 1107 24-25 0.05539433173000674 1107 26-27 -0.04675845903751252 1107 28-29 -0.10485449042968753 1107 30-31 -0.07055788109548189 1107 32-33 -0.08031022144831468 1107 34-35 -0.0037760523036070026 1107 36-37 0.12941156716876634 1107 38-39 0.10999896758887928 1107 40-41 0.11197047486225387 1107 42-43 -0.13452683841823188 1107 44-45 -0.0302767964039532 1107 46-47 0.03990742893450516 1107 48-49 -0.05596094377263228 1107 50-51 0.04250622761660594 1107 52-53 0.010231593844935105 1107 54-55 0.1363443853293944 1107 56-57 0.015468954816945768 1107 58-59 0.06945174863327708 1107 60-61 0.06519893982405023 1107 62-63 0.1420433331344455 1107 64-65 0.1411735490735353 1107 66-67 0.3895833843574579 1107 68-69 0.2611178135973695 1107 70-71 0.14773042155858462 1107 72-73 0.27597541922230917 1107 74-75 0.26476441359515235 1107 76-77 0.07218640964269696 1107 78-79 0.2839078729909801 1107 80-81 0.42595134377850385 1107 82-83 0.3525847657840373 1107 84-85 0.15542400845911786 1107 86-87 0.20203154029058012 1107 88-89 0.1326673756100334 1107 90-91 0.2667724300025398 1107 92-93 0.04447181694920488 1107 94-95 0.4441188807441705 1107 96-97 0.7105243530708165 1107 98-99 0.8638828855753253 1107 100-101 0.787874949692732 1108 1 -0.06326477161269395 1108 2 0.007232793262758719 1108 3 -0.05571675300933876 1108 4 -0.03611643044254009 1108 5 -0.1573258217547462 1108 6 -0.08594087095783465 1108 7 -0.02676911446389596 1108 8 -0.06981787324833988 1108 9 0.04051523598447204 1108 10-11 -0.10273234268235854 1108 12-13 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#Quality Count 2 5.0 3 16.0 4 42.0 5 85.0 6 157.0 7 399.0 8 1105.0 9 1940.0 10 3217.0 11 5222.0 12 7268.0 13 9871.0 14 12573.0 15 16570.0 16 20590.0 17 25749.0 18 22863.0 19 15982.0 20 14479.0 21 15187.0 22 16479.0 23 18571.0 24 21554.0 25 25854.0 26 32097.0 27 38753.0 28 48254.0 29 59244.0 30 71792.0 31 87697.0 32 108017.0 33 135364.0 34 174512.0 35 231189.0 36 319397.0 37 405172.0 38 238480.0 39 13550.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.271602016691574 19.55753588597624 15.304768794920859 21.86609330241133 2 15.60312834017365 21.37688604341392 39.244708443958984 23.775277172453446 3 20.444008249845776 24.808748044897403 29.974725046447066 24.772518658809755 4 13.035618292591158 17.26644800726001 37.476146553771244 32.22178714637759 5 13.206823663742743 38.10484225267959 34.03680568040853 14.65152840316914 6 30.856670376561873 36.91072369992205 17.165354685507527 15.067251238008552 7 27.928934252603415 33.205605198402914 20.640905656160488 18.22455489283318 8 26.164005989283996 33.98693370017622 19.420023547997406 20.42903676254237 9 25.70577071080839 17.543604310594986 20.965756399834902 35.784868578761724 10-11 25.09009646337023 26.726770027143843 26.835160646117135 21.347972863368792 12-13 26.792365431217597 24.169435590197516 27.34247776525116 21.69572121333373 14-15 22.94593431549919 25.74274133770719 26.988998510754787 24.322325836038832 16-17 21.45350758830112 28.784648427827836 27.518065318351315 22.243778665519727 18-19 21.204169608664365 27.72454520508071 29.945406135366287 21.12587905088864 20-21 22.679839606866498 25.931657637531163 29.860987510909986 21.52751524469235 22-23 21.8447528294809 26.053911932653456 30.105699290419025 21.995635947446623 24-25 22.31650478568559 26.229337560540618 30.148456173402614 21.305701480371177 26-27 21.963799895682776 25.928665494318317 30.801631674584335 21.30590293541457 28-29 21.277951902753774 27.20432193436213 30.234592192697207 21.283133970186892 30-31 21.768899370828297 26.146110404921984 30.031875389486707 22.05311483476301 32-33 21.712349445092798 26.309765735888508 30.156423901337813 21.82146091768088 34-35 22.61834699496645 26.15751359779371 29.85446868339109 21.36967072384875 36-37 22.384931440487684 25.92361847315809 30.303653690812425 21.3877963955418 38-39 21.820608678097106 26.62538574127215 30.230917523468932 21.32308805716181 40-41 22.010562961414234 26.672221060675973 29.74486135944169 21.572354618468104 42-43 21.693067812381013 26.758235197372127 29.136140693962243 22.412556296284613 44-45 22.23655876186369 26.335494853940776 29.127988425027855 22.299957959167674 46-47 22.835366973272837 27.005697526220583 28.657347046687992 21.501588453818588 48-49 22.59848004895277 26.935107841184667 28.552905849729775 21.91350626013278 50-51 21.96503909603074 27.358862946028868 28.917116775313062 21.75898118262733 52-53 22.580762899781593 27.641722875773407 27.905371336081718 21.872142888363282 54-55 22.120698088504962 27.13083335379838 28.205529707017945 22.542938850678716 56-57 22.67651375960762 27.222585830685126 27.880077548109906 22.22082286159735 58-59 23.270401284745628 26.87825106611538 27.844085808319125 22.007261840819872 60-61 22.513031086477746 27.217808118870995 28.72415327509195 21.545007519559302 62-63 22.097231785262384 27.292433462269152 28.656060403966848 21.954274348501617 64-65 22.007079462573707 27.862416886163054 28.100193998003693 22.03030965325955 66-67 22.326898640062726 27.29366624309442 27.644442642009352 22.7349924748335 68-69 22.218965333890882 27.274709944178788 28.037632906578324 22.468691815352003 70-71 22.861556741767828 27.121241853552174 27.748062391871652 22.269139012808346 72-73 22.558131204043132 26.840713505373504 28.44890106807716 22.152254222506198 74-75 22.044825828790714 28.207836544072617 27.552094997807735 22.19524262932893 76-77 22.370974079461295 27.358055265667048 27.576395385095104 22.694575269776553 78-79 22.47369127795006 27.46072279254118 27.726130641944536 22.33945528756422 80-81 22.85230390160022 27.33546714299619 27.503343559277432 22.308885396126158 82-83 22.65651614368447 27.257574364769944 27.958931973561597 22.12697751798399 84-85 22.33600324444895 27.68900604954544 27.69290389446529 22.282086811540324 86-87 22.304786563879432 27.78574106484644 27.386297478564465 22.523174892709665 88-89 22.24902009028959 27.73232354640342 27.019371073263628 22.99928529004336 90-91 22.45587501991316 27.242470120114405 27.333524259359866 22.96813060061257 92-93 22.75969435861157 27.53428777738601 27.496775399991215 22.20924246401121 94-95 22.384464622983185 27.319905490359442 27.672683203675845 22.622946682981528 96-97 21.844597963428956 27.392121596768664 28.110623788593497 22.652656651208876 98-99 22.015522179837298 27.714595927427375 27.128239016425425 23.141642876309895 100-101 21.811853111575846 27.343082876070046 27.37379240359394 23.47127160876017 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 499.0 1 384.0 2 435.0 3 577.0 4 447.5 5 375.5 6 627.0 7 1245.0 8 1956.5 9 2378.0 10 2321.5 11 2053.5 12 2099.0 13 2350.0 14 2622.0 15 2892.0 16 3148.0 17 3459.0 18 3810.5 19 4200.5 20 4622.5 21 5316.0 22 6295.0 23 7512.0 24 9070.0 25 11032.5 26 14075.0 27 17960.5 28 21160.0 29 24589.0 30 28655.5 31 32755.5 32 37660.0 33 41909.0 34 45714.0 35 49688.0 36 54641.0 37 58975.5 38 60505.5 39 61468.5 40 62921.5 41 64797.0 42 65876.0 43 67349.0 44 70516.5 45 73935.0 46 76530.5 47 87023.5 48 110631.5 49 109390.0 50 85883.5 51 75342.0 52 71711.0 53 69909.0 54 68175.0 55 63618.0 56 57193.0 57 51237.5 58 47769.0 59 43213.0 60 35726.5 61 29806.0 62 24724.5 63 18776.0 64 13376.5 65 9816.5 66 7445.5 67 5766.0 68 4470.5 69 3513.0 70 2755.0 71 2102.5 72 1677.5 73 1437.0 74 1095.0 75 786.5 76 565.5 77 379.5 78 271.5 79 188.0 80 121.0 81 87.0 82 63.0 83 34.5 84 22.5 85 14.0 86 6.0 87 3.5 88 3.5 89 2.5 90 1.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03658816282813882 2 0.004550990696603474 3 0.004686168638086746 4 7.209490212441148E-4 5 9.011862765551434E-4 6 3.154151967943002E-4 7 0.0 8 0.0 9 9.462455903829005E-4 10-11 4.0553382444981454E-4 12-13 0.001036364218038415 14-15 0.002343084319043373 16-17 0.0026359698589237942 18-19 0.0 20-21 0.0 22-23 0.0013292497579188364 24-25 0.0010138345611245363 26-27 0.0047086982950006245 28-29 0.004753757608828381 30-31 0.001351779414832715 32-33 3.154151967943002E-4 34-35 0.008426091685790591 36-37 0.010791705661747843 38-39 0.008696447568757135 40-41 0.0014869573563159866 42-43 5.407117659330861E-4 44-45 0.0011715421595216865 46-47 2.2529656913878587E-5 48-49 6.758897074163575E-4 50-51 8.110676488996291E-4 52-53 0.002433202946698887 54-55 0.0029513850557180946 56-57 0.0013292497579188364 58-59 0.0011264828456939292 60-61 0.0038075120184454807 62-63 0.004731227951914503 64-65 0.009101981393206948 66-67 0.0034695671647373017 68-69 0.0031090926541152445 70-71 6.758897074163576E-5 72-73 0.0012841904440910792 74-75 0.005857710797608432 76-77 0.0023881436328711298 78-79 0.003199211281770759 80-81 0.004731227951914503 82-83 0.004550990696603474 84-85 0.005497236286986375 86-87 0.0038300416753593593 88-89 0.00968775247296779 90-91 0.012954552725480186 92-93 0.0013292497579188364 94-95 0.0023656139759572516 96-97 0.0012391311302633221 98-99 0.003852571332273238 100-101 0.005226880404019831 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2219297.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.41873051903049 #Duplication Level Percentage of deduplicated Percentage of total 1 79.54904500252182 46.47154223048552 2 12.436285725602104 14.530240489232298 3 3.539063061806841 6.202427138926464 4 1.4623606548656907 3.4171701207292697 5 0.7807079656670426 2.280398413018174 6 0.4653208133497684 1.6310070719985723 7 0.31320809562370894 1.2808053534234132 8 0.22272506177065535 1.0409052290731469 9 0.17301336508084017 0.9096499035763418 >10 0.948169436521147 10.228472756811376 >50 0.06924702551148466 2.7672231142296853 >100 0.03638638946393371 3.7808622562589704 >500 0.002387749459722669 0.9399778434769746 >1k 0.00169453187464173 2.3688431579627425 >5k 3.080967044803145E-4 1.4281003732058415 >10k+ 7.702417612007863E-5 0.7223745475911079 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16054 0.7233822241908135 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9798 0.44149115688436474 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8562 0.38579784499325687 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8142 0.3668729331855989 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5236 0.2359305672021365 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4845 0.21831237549548346 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4685 0.21110288528304236 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4223 0.19028548229461853 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3865 0.17415424794428144 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3671 0.16541274106169657 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3055 0.13765620374379817 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3037 0.1368451360948985 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2912 0.13121272186642888 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2805 0.12639137528685887 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2665 0.12008307135097285 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2362 0.10643009926116244 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.505931382775717E-5 7 0.0 0.0 0.0 0.0 4.505931382775717E-5 8 0.0 0.0 0.0 0.0 4.505931382775717E-5 9 0.0 0.0 0.0 0.0 9.011862765551433E-5 10-11 0.0 0.0 0.0 0.0 9.011862765551433E-5 12-13 0.0 0.0 0.0 0.0 1.351779414832715E-4 14-15 0.0 0.0 0.0 6.758897074163575E-5 2.0276691222490727E-4 16-17 0.0 0.0 0.0 9.011862765551433E-5 2.2529656913878584E-4 18-19 0.0 0.0 0.0 1.1264828456939292E-4 2.2529656913878584E-4 20-21 0.0 0.0 0.0 1.8023725531102867E-4 2.2529656913878584E-4 22-23 0.0 0.0 0.0 3.1541519679430014E-4 2.478262260526644E-4 24-25 0.0 0.0 0.0 4.0553382444981454E-4 2.70355882966543E-4 26-27 0.0 0.0 0.0 4.956524521053288E-4 2.70355882966543E-4 28-29 0.0 0.0 0.0 6.984193643302362E-4 2.70355882966543E-4 30-31 0.0 0.0 0.0 0.0013968387286604723 2.70355882966543E-4 32-33 0.0 0.0 0.0 0.0025458512312682797 2.70355882966543E-4 34-35 0.0 0.0 0.0 0.003424507850909545 2.70355882966543E-4 36-37 0.0 0.0 0.0 0.004573520353517353 2.70355882966543E-4 38-39 0.0 0.0 0.0 0.0055197659439002525 2.70355882966543E-4 40-41 0.0 0.0 0.0 0.006961663986388483 2.70355882966543E-4 42-43 0.0 0.0 0.0 0.00973281178679555 2.70355882966543E-4 44-45 0.0 0.0 0.0 0.013698031403638178 2.70355882966543E-4 46-47 0.0 0.0 0.0 0.02289013142450064 2.70355882966543E-4 48-49 0.0 0.0 0.0 0.04251346259648889 2.70355882966543E-4 50-51 0.0 0.0 0.0 0.057608332728787535 2.70355882966543E-4 52-53 0.0 0.0 0.0 0.07288344011639722 2.70355882966543E-4 54-55 0.0 0.0 0.0 0.09264194922986874 2.928855398804216E-4 56-57 0.0 0.0 0.0 0.10980954779824421 3.154151967943002E-4 58-59 0.0 0.0 0.0 0.13281232750731425 3.154151967943002E-4 60-61 0.0 0.0 0.0 0.1696933758753335 3.154151967943002E-4 62-63 0.0 0.0 0.0 0.1982835104990454 3.154151967943002E-4 64-65 0.0 0.0 0.0 0.23149222479010245 3.154151967943002E-4 66-67 0.0 0.0 0.0 0.29160134943633054 3.154151967943002E-4 68-69 0.0 0.0 0.0 0.36078992581885166 3.154151967943002E-4 70-71 0.0 2.2529656913878584E-5 0.0 0.42137217326027115 3.154151967943002E-4 72-73 2.2529656913878584E-5 4.505931382775717E-5 0.0 0.4842524457069063 3.154151967943002E-4 74-75 4.505931382775717E-5 4.505931382775717E-5 0.0 0.5388192747523202 3.154151967943002E-4 76-77 4.505931382775717E-5 4.505931382775717E-5 0.0 0.584689656228977 3.154151967943002E-4 78-79 4.505931382775717E-5 6.758897074163575E-5 0.0 0.6510394958403494 3.154151967943002E-4 80-81 4.505931382775717E-5 9.011862765551433E-5 0.0 0.7464525928706253 3.3794485370817876E-4 82-83 4.505931382775717E-5 9.011862765551433E-5 0.0 0.854324590174276 3.6047451062205734E-4 84-85 4.505931382775717E-5 9.011862765551433E-5 0.0 0.9808060840887902 3.6047451062205734E-4 86-87 4.505931382775717E-5 9.011862765551433E-5 0.0 1.1837081742551807 3.6047451062205734E-4 88-89 4.505931382775717E-5 9.011862765551433E-5 0.0 1.4392170133154778 3.6047451062205734E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1810 0.0 24.928255 2 GTACCTG 1890 0.0 24.886122 1 GGACCGT 235 4.660251E-9 22.23215 6 GAGTACT 10720 0.0 21.977474 12-13 GTACATG 10170 0.0 21.255646 1 TACATGG 10270 0.0 20.348345 2 GTACTTT 11480 0.0 20.294724 14-15 ACATGGG 10120 0.0 19.898594 3 AGTACTT 11280 0.0 18.844076 12-13 AGAGTAC 17670 0.0 17.688658 10-11 ACTTTTT 13135 0.0 17.664482 16-17 CTAATAC 720 0.0 17.150545 3 ACACCGT 475 1.8189894E-12 16.998552 6 CATGGGA 5380 0.0 16.77291 4 AGTACAT 8110 0.0 16.339176 2 TAGCCCT 1345 0.0 16.243238 4 TACTTTT 12825 0.0 16.184902 14-15 GAGTACA 9610 0.0 16.067335 1 CATGGGG 4560 0.0 16.03958 4 TATACGG 180 0.0026215212 15.831629 5 >>END_MODULE