FastQCFastQC Report
Thu 26 May 2016
SRR936728_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936728_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2245902
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT139050.6191276378043209No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT92650.41252913083473813No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86360.38452256598907697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71380.31782330662691427No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC54210.24137295394010957No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT51630.22988536454395608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40900.18210945980724003No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40320.17952697847012025No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC32400.14426275055634663No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT31960.1423036267833592No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC31000.138029174915023No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG26560.1182598350239681No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA25270.11251604032589133No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC24500.10908757372316334No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA23220.10338830456538176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG98200.024.6857221
TACCTGG16900.023.6227972
GTACCTG17950.023.5674551
TACATGG98500.023.498032
ACATGGG98050.022.396573
GTATAGG7850.021.1926631
GAGTACT95300.021.07109312-13
GTACTTT101400.019.7086614-15
AGTACTT98050.019.34098212-13
GGACCGT2001.2774179E-519.0141166
CATGGGG46900.018.9528834
AGAGTAC158450.018.18011310-11
TAGCCCT16500.017.8613474
TAAGGTG10150.017.3285225
CATGGGA50900.017.2766864
ACTTTTT120950.016.9346516-17
GTGTAGG10150.016.858681
TACTTTT116850.016.30965414-15
AGTACAT75050.016.1483522
GTGTAGC13650.016.0181481
Produced by FastQC (version 0.11.5)