##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936728_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2245902 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.14663774287569 31.0 31.0 34.0 28.0 34.0 2 31.188680539044 31.0 31.0 34.0 28.0 34.0 3 31.134873204618902 31.0 31.0 34.0 27.0 34.0 4 34.79723959460386 37.0 35.0 37.0 32.0 37.0 5 34.83657479266682 37.0 35.0 37.0 32.0 37.0 6 34.897001293912204 37.0 35.0 37.0 32.0 37.0 7 34.76280398699498 37.0 35.0 37.0 32.0 37.0 8 34.74748942741046 37.0 35.0 37.0 32.0 37.0 9 36.19541413650284 39.0 35.0 39.0 32.0 39.0 10-11 36.063655938682984 39.0 35.0 39.0 30.0 39.0 12-13 35.956425970500945 39.0 35.0 39.0 30.0 39.0 14-15 36.87489302738944 39.0 36.0 41.0 30.5 41.0 16-17 36.79754036462855 39.0 36.0 41.0 30.0 41.0 18-19 36.69826911414657 39.0 36.0 41.0 30.0 41.0 20-21 36.67862311000213 39.0 36.0 41.0 30.0 41.0 22-23 36.66362624014761 39.0 36.0 41.0 30.0 41.0 24-25 36.76217751264303 39.0 36.0 41.0 30.0 41.0 26-27 36.67164462207167 39.0 36.0 41.0 30.0 41.0 28-29 36.55293997690015 39.0 36.0 41.0 30.0 41.0 30-31 36.352623578410814 39.0 35.5 41.0 30.0 41.0 32-33 36.154472234318334 39.0 35.0 40.0 29.5 41.0 34-35 35.924384055938326 38.0 35.0 40.0 28.5 41.0 36-37 35.693211458024436 38.0 35.0 40.0 27.0 41.0 38-39 35.48229241525232 38.0 35.0 40.0 27.0 41.0 40-41 35.25825614830923 38.0 34.0 40.0 26.5 41.0 42-43 34.96800928980873 38.0 34.0 40.0 25.0 41.0 44-45 34.77689988254163 38.0 34.0 40.0 24.0 41.0 46-47 34.592437915812894 38.0 33.5 40.0 24.0 41.0 48-49 34.31987682454533 38.0 33.0 40.0 23.0 41.0 50-51 33.32787472472085 37.0 32.0 39.5 21.5 40.5 52-53 33.43850043323351 37.5 33.0 39.5 21.5 40.5 54-55 33.88355458074306 38.0 33.0 40.0 22.0 41.0 56-57 33.718371505079034 38.0 33.0 40.0 20.5 41.0 58-59 33.48010643385152 37.5 33.0 40.0 19.5 41.0 60-61 33.163244433639576 37.0 32.0 40.0 18.0 41.0 62-63 32.84427281332845 36.5 32.0 40.0 17.5 41.0 64-65 32.48856316971978 36.0 31.0 39.0 13.5 41.0 66-67 32.099620330717904 36.0 31.0 39.0 12.5 41.0 68-69 31.648695490720435 35.0 31.0 39.0 8.0 40.0 70-71 31.24110491018753 35.0 30.0 38.0 7.5 40.0 72-73 30.815263533315346 35.0 30.0 37.0 7.0 39.5 74-75 30.403790993551812 35.0 29.5 37.0 2.0 39.0 76-77 29.9466597384926 34.0 29.0 36.0 2.0 39.0 78-79 29.470208628871607 34.0 29.0 36.0 2.0 38.5 80-81 28.99916269721475 34.0 28.5 35.0 2.0 37.0 82-83 28.577135155496546 34.0 27.5 35.0 2.0 37.0 84-85 28.186928013777983 34.0 27.0 35.0 2.0 36.0 86-87 27.8522295273792 34.0 26.5 35.0 2.0 36.0 88-89 27.47584021920814 33.5 26.0 35.0 2.0 36.0 90-91 27.08781327057013 33.0 25.5 35.0 2.0 35.0 92-93 26.64218184943065 33.0 25.0 35.0 2.0 35.0 94-95 26.1318862087482 33.0 24.0 35.0 2.0 35.0 96-97 25.50489068534602 32.0 21.5 35.0 2.0 35.0 98-99 24.767904610263493 32.0 10.0 35.0 2.0 35.0 100-101 22.24659713558294 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.544155082291546 1101 2 0.5656726775707739 1101 3 0.6289232817710584 1101 4 0.7904262917054652 1101 5 0.5470405751126322 1101 6 0.49189458151190024 1101 7 0.6195911358620521 1101 8 0.5487788134828051 1101 9 0.735469373748117 1101 10-11 0.7081895086670897 1101 12-13 0.8086993084615557 1101 14-15 1.0413674655419598 1101 16-17 1.022610344278469 1101 18-19 1.0946550414086715 1101 20-21 0.9706555399770878 1101 22-23 1.0221049786251726 1101 24-25 0.9195817366092456 1101 26-27 0.8996209488999796 1101 28-29 0.8816637526613249 1101 30-31 0.7412029546070116 1101 32-33 0.7225097808536134 1101 34-35 0.6615365493246443 1101 36-37 0.7084461769891348 1101 38-39 0.7699769196901016 1101 40-41 0.827294105218968 1101 42-43 0.9627291345554951 1101 44-45 1.034895220478269 1101 46-47 1.0646703778657027 1101 48-49 1.027119325817715 1101 50-51 1.0382771970120572 1101 52-53 0.9756491827876346 1101 54-55 0.9560268174159958 1101 56-57 1.0340987196670355 1101 58-59 1.1214203904936326 1101 60-61 0.9681041532647114 1101 62-63 1.21609405884913 1101 64-65 0.9816268680023015 1101 66-67 1.0649501913364645 1101 68-69 0.9982794985805583 1101 70-71 0.8818534877632551 1101 72-73 0.9931928087261355 1101 74-75 0.8294506387351959 1101 76-77 0.861802002487714 1101 78-79 0.8330704118629519 1101 80-81 0.7830090435859631 1101 82-83 0.8387849557387916 1101 84-85 0.7867805792488198 1101 86-87 0.7325396024969493 1101 88-89 0.8642564327000315 1101 90-91 0.8488453749318836 1101 92-93 1.060201235913837 1101 94-95 1.2343045126907732 1101 96-97 1.1931526894000193 1101 98-99 1.290791266914379 1101 100-101 1.4382763635102336 1102 1 0.2969470248360899 1102 2 0.38171926768240283 1102 3 0.5797379763791461 1102 4 0.4180131515674077 1102 5 0.436279430539912 1102 6 0.3380057602106632 1102 7 0.49356177029009274 1102 8 0.4481015165395732 1102 9 0.4458292195383109 1102 10-11 0.5418173637582626 1102 12-13 0.4278799262492967 1102 14-15 0.6144013008338476 1102 16-17 0.3722688433790964 1102 18-19 0.507059562403569 1102 20-21 0.6305715755011576 1102 22-23 0.3263478446346184 1102 24-25 0.531295866986504 1102 26-27 0.3977345662526446 1102 28-29 0.35172296974894834 1102 30-31 0.29454953970111575 1102 32-33 0.4124249365927355 1102 34-35 0.0900641604816812 1102 36-37 0.12273879896134332 1102 38-39 0.07317993831473757 1102 40-41 0.3628400805121288 1102 42-43 0.09489568746872123 1102 44-45 0.2567299020132978 1102 46-47 0.28899122512610376 1102 48-49 0.4763430187012858 1102 50-51 0.3794313373968734 1102 52-53 0.14887815163155693 1102 54-55 0.4104989773773937 1102 56-57 0.26266788004289765 1102 58-59 0.25878944515782365 1102 60-61 0.36861147300674446 1102 62-63 0.31308253091699356 1102 64-65 0.4870908136590799 1102 66-67 0.27214341273090525 1102 68-69 0.26418023684384906 1102 70-71 0.06253459596237221 1102 72-73 0.2854358891831552 1102 74-75 0.05731725990048275 1102 76-77 0.20598255154225598 1102 78-79 0.025483074305277853 1102 80-81 0.17144921924052525 1102 82-83 0.13228903264849023 1102 84-85 0.15217210760710742 1102 86-87 0.24929374934267656 1102 88-89 0.4284206782397604 1102 90-91 0.6109407855474558 1102 92-93 0.48604833822526317 1102 94-95 0.6333862199684503 1102 96-97 0.5254057945941177 1102 98-99 0.577068907111201 1102 100-101 1.200162223785263 1103 1 0.285899223413967 1103 2 0.3330219474560714 1103 3 0.5128067002834484 1103 4 0.3866325898938783 1103 5 0.3988503918598738 1103 6 0.34204329208459683 1103 7 0.4204640395214554 1103 8 0.23179038031314292 1103 9 0.47942623046014887 1103 10-11 0.35016539021987114 1103 12-13 0.3125912853187316 1103 14-15 0.6595100366390838 1103 16-17 0.708540469567275 1103 18-19 0.80979840810609 1103 20-21 0.7099963370331821 1103 22-23 0.5403205603440497 1103 24-25 0.4795484330423463 1103 26-27 0.40692650742954584 1103 28-29 0.3893957013662259 1103 30-31 0.6322738387074409 1103 32-33 0.7546006045060665 1103 34-35 0.7130865785679106 1103 36-37 0.5063048261857332 1103 38-39 0.3837769452429711 1103 40-41 0.667991477973402 1103 42-43 0.7625782267075252 1103 44-45 0.7260470559327175 1103 46-47 0.8110998903776974 1103 48-49 0.802801698562817 1103 50-51 0.8351362980499104 1103 52-53 0.9535264384094759 1103 54-55 1.016862582881373 1103 56-57 0.8904232159947014 1103 58-59 0.8155421107209264 1103 60-61 0.8628819849799001 1103 62-63 0.7574836836037164 1103 64-65 0.8087378684107023 1103 66-67 0.9466815526307855 1103 68-69 0.928997033668665 1103 70-71 0.8141931888964926 1103 72-73 0.7425181434118819 1103 74-75 0.8092605633949539 1103 76-77 0.9062358291249026 1103 78-79 0.733871153269444 1103 80-81 0.8304242079752377 1103 82-83 0.5726345158510675 1103 84-85 0.7248120717179383 1103 86-87 0.6732326825050876 1103 88-89 0.5916945301955927 1103 90-91 0.6040370952592937 1103 92-93 0.5667459772741772 1103 94-95 0.7137111228074353 1103 96-97 0.9358132385355908 1103 98-99 0.9974255790560775 1103 100-101 1.2818533545937072 1104 1 0.2559702621351896 1104 2 0.3145271494558024 1104 3 0.394561581892404 1104 4 0.45374833592328656 1104 5 0.32841158956862415 1104 6 0.29342398183577956 1104 7 0.3575180821774424 1104 8 0.4098878528086729 1104 9 0.5664218453033527 1104 10-11 0.5248944399387838 1104 12-13 0.5382446573036788 1104 14-15 0.6772823278528008 1104 16-17 0.5037670769462963 1104 18-19 0.5226335286671926 1104 20-21 0.758244362484291 1104 22-23 0.43133913686728675 1104 24-25 0.6393209453564026 1104 26-27 0.3299911219574767 1104 28-29 0.09695733308675614 1104 30-31 0.2059860725169571 1104 32-33 0.25744608402767 1104 34-35 0.28950835613277803 1104 36-37 0.40981000361875886 1104 38-39 0.17097687318063493 1104 40-41 0.26207802647421374 1104 42-43 0.43609138831958916 1104 44-45 0.47405686623681476 1104 46-47 0.49605073649595255 1104 48-49 0.5397912945633578 1104 50-51 0.3451366665504807 1104 52-53 0.33997134962131526 1104 54-55 0.2681016046351914 1104 56-57 0.4601879079077449 1104 58-59 0.4309477274885225 1104 60-61 0.28076851137068815 1104 62-63 0.4609700265631105 1104 64-65 0.523364884415912 1104 66-67 0.401147492941881 1104 68-69 0.25909738865755116 1104 70-71 0.3124313467211941 1104 72-73 0.5365047054243881 1104 74-75 0.525381398826692 1104 76-77 0.5455324843680529 1104 78-79 0.3657390826647351 1104 80-81 0.6273431337551294 1104 82-83 0.5570392441228371 1104 84-85 0.7142171889625324 1104 86-87 0.5867448614489632 1104 88-89 0.4758115823548046 1104 90-91 0.723893216258503 1104 92-93 0.5087614297314609 1104 94-95 0.5052180854210029 1104 96-97 0.3606033365157977 1104 98-99 0.3783693499633678 1104 100-101 1.0929253227552564 1105 1 0.4112499232207867 1105 2 0.35517938147932426 1105 3 0.46881461746059827 1105 4 0.48246306946560225 1105 5 0.5265532305169955 1105 6 0.5868315134469952 1105 7 0.41097308124591336 1105 8 0.4854673851003142 1105 9 0.45263603207888536 1105 10-11 0.6564639441225637 1105 12-13 0.5922267395536096 1105 14-15 0.7670247399448513 1105 16-17 0.8217380456550316 1105 18-19 0.623787328088838 1105 20-21 0.7582207907606318 1105 22-23 0.5975988470403948 1105 24-25 0.5030948512153159 1105 26-27 0.46731107367999414 1105 28-29 0.5804194603740669 1105 30-31 0.329939236879099 1105 32-33 0.5783851458568705 1105 34-35 0.4735559693945035 1105 36-37 0.5379925508229562 1105 38-39 0.42225104229763133 1105 40-41 0.4356004196116814 1105 42-43 0.21616769266213254 1105 44-45 0.21124932004514818 1105 46-47 0.6067013484697412 1105 48-49 0.7454555554561608 1105 50-51 0.6959910807809919 1105 52-53 0.9735335149797848 1105 54-55 0.8109457639508832 1105 56-57 0.5910560115585284 1105 58-59 0.6568352312348864 1105 60-61 0.6870232803920899 1105 62-63 0.7543749660947512 1105 64-65 0.7796405520789094 1105 66-67 0.7323765436737446 1105 68-69 0.7706147272398454 1105 70-71 0.8226016139483967 1105 72-73 0.6723072375621229 1105 74-75 0.7253729745680673 1105 76-77 0.5541658104111598 1105 78-79 0.5825867898893442 1105 80-81 0.6823993089797078 1105 82-83 0.5513757932548984 1105 84-85 0.5402940765229829 1105 86-87 0.6744331314788496 1105 88-89 0.6593129148457102 1105 90-91 0.709935515757099 1105 92-93 0.7820969501319333 1105 94-95 0.8252514584455568 1105 96-97 0.9576956712504732 1105 98-99 1.2450482480055385 1105 100-101 1.633917522215782 1106 1 0.22098564406795873 1106 2 0.09012928673488219 1106 3 0.09718435711690532 1106 4 0.36023565049937645 1106 5 0.433654399916243 1106 6 0.1816094119694185 1106 7 0.3774565544463684 1106 8 0.4075557774309715 1106 9 0.5965305775082612 1106 10-11 0.49846740759313235 1106 12-13 0.5137456599316863 1106 14-15 0.34382270075569465 1106 16-17 0.20076616773083344 1106 18-19 0.4007316492067474 1106 20-21 0.05750602971143337 1106 22-23 0.00794215845181867 1106 24-25 -0.2390529290223995 1106 26-27 -0.3247775263006929 1106 28-29 0.026042263774641583 1106 30-31 -0.0984538881252277 1106 32-33 0.016856713130039225 1106 34-35 0.17759065881434566 1106 36-37 -0.07913293999742876 1106 38-39 0.06683932009117655 1106 40-41 0.055096746348667125 1106 42-43 0.2681714989200401 1106 44-45 0.07217192558491803 1106 46-47 0.309527053301494 1106 48-49 0.2672142292119375 1106 50-51 0.26903832182080833 1106 52-53 0.34063395595592993 1106 54-55 0.5215500222266414 1106 56-57 0.4976622817323104 1106 58-59 0.44375919605831626 1106 60-61 0.5303115371899096 1106 62-63 0.5592009174697026 1106 64-65 0.4357999626956257 1106 66-67 0.38223904969311917 1106 68-69 0.5420290480380459 1106 70-71 0.25558545251401554 1106 72-73 0.5433828580978535 1106 74-75 0.669588949051505 1106 76-77 0.5087937032149661 1106 78-79 0.5340311276522165 1106 80-81 0.7728960737593624 1106 82-83 0.7458950026949971 1106 84-85 0.8425966680941706 1106 86-87 0.8411812101393181 1106 88-89 0.6821975131796982 1106 90-91 0.9783233374457154 1106 92-93 1.2555252454471848 1106 94-95 1.2433396213723817 1106 96-97 1.1710122343722915 1106 98-99 1.3514936882911606 1106 100-101 1.849737339345328 1107 1 0.12512821723201029 1107 2 0.2184784528830903 1107 3 0.27054058590634256 1107 4 0.28072729582800804 1107 5 0.12267562732611026 1107 6 0.1821928936614583 1107 7 0.2751114834389625 1107 8 0.2116010546965441 1107 9 0.3208482049698844 1107 10-11 0.4388726499868696 1107 12-13 0.3682477449486896 1107 14-15 0.2173352589101043 1107 16-17 0.1424261568280798 1107 18-19 0.24706915126875373 1107 20-21 0.18109205336691048 1107 22-23 0.1790705999699327 1107 24-25 0.025535051483174698 1107 26-27 0.00172681954470022 1107 28-29 0.08814431204666562 1107 30-31 0.013911616163021279 1107 32-33 0.11914076004548946 1107 34-35 0.14894816910970832 1107 36-37 0.2075313216020831 1107 38-39 0.2182663619548464 1107 40-41 0.15434215245013405 1107 42-43 0.04737099662976618 1107 44-45 -0.04008719445746323 1107 46-47 0.09380998840368449 1107 48-49 0.176191982667099 1107 50-51 0.1412621131562659 1107 52-53 0.2885532383778724 1107 54-55 0.2582511348720615 1107 56-57 0.49079617397453745 1107 58-59 0.4784392364647445 1107 60-61 0.4392017520746663 1107 62-63 0.4704993812452969 1107 64-65 0.4706323185829149 1107 66-67 0.4778331265707507 1107 68-69 0.49628145640930654 1107 70-71 0.4616351754010033 1107 72-73 0.6421842078724502 1107 74-75 0.5696194121476772 1107 76-77 0.40204168113925576 1107 78-79 0.5658184792506837 1107 80-81 0.5242246122959955 1107 82-83 0.5111462530770119 1107 84-85 0.5556198619809258 1107 86-87 0.7097063541047781 1107 88-89 0.5433551402040386 1107 90-91 0.4813437036653241 1107 92-93 0.7020868234061979 1107 94-95 0.7380732748700787 1107 96-97 0.8618992652353086 1107 98-99 1.1174862834907806 1107 100-101 1.5094715712665732 1108 1 0.09025944723162382 1108 2 0.14826136142724522 1108 3 0.16218942540163184 1108 4 0.3715302739193902 1108 5 0.30309799231103796 1108 6 0.2635825693584479 1108 7 0.4718879755094463 1108 8 0.3228705629412971 1108 9 -0.11989686070234029 1108 10-11 0.17385147320388938 1108 12-13 0.11154146392009068 1108 14-15 0.541976551805682 1108 16-17 0.6663890961498637 1108 18-19 0.49750897173043995 1108 20-21 0.4614820940004307 1108 22-23 0.44517702147947347 1108 24-25 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0.8862337550899824 1108 92-93 0.9707543407194272 1108 94-95 0.9908317176244239 1108 96-97 1.3836682525625896 1108 98-99 1.4493099753273881 1108 100-101 1.5934667335684978 1109 1 0.2360410961338708 1109 2 0.20898465519826814 1109 3 0.24405923372487592 1109 4 0.29583404915614153 1109 5 0.2610311782400956 1109 6 0.3015082292552478 1109 7 0.36047214653402193 1109 8 0.2654344824615009 1109 9 0.47658877606209415 1109 10-11 0.43589704810394636 1109 12-13 0.3265758819948559 1109 14-15 0.16599564669177624 1109 16-17 0.11348574723245264 1109 18-19 0.29257100640361955 1109 20-21 0.17270407923017927 1109 22-23 0.2012447597765643 1109 24-25 0.08453424577035662 1109 26-27 0.08244332948061839 1109 28-29 -0.01311747252918849 1109 30-31 0.1442316822421006 1109 32-33 0.14698001269415073 1109 34-35 0.2015340856615495 1109 36-37 0.15587920802602895 1109 38-39 0.18799259177722405 1109 40-41 0.21420144959885334 1109 42-43 0.2690638367602176 1109 44-45 0.216521316167821 1109 46-47 0.14490613277737197 1109 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-5.374761956627271 2316 98-99 -7.143392180687048 2316 100-101 -7.963869076261362 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6544.0 3 2622.0 4 2709.0 5 2917.0 6 3188.0 7 3784.0 8 4888.0 9 6248.0 10 7667.0 11 9598.0 12 11799.0 13 13980.0 14 15779.0 15 18354.0 16 21819.0 17 25839.0 18 21830.0 19 16854.0 20 17270.0 21 18507.0 22 20387.0 23 22887.0 24 26265.0 25 30059.0 26 34930.0 27 40870.0 28 48307.0 29 56516.0 30 67670.0 31 82491.0 32 100253.0 33 125425.0 34 161499.0 35 214337.0 36 289569.0 37 366447.0 38 282579.0 39 43204.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.88672669054432 18.487330553448906 14.498235019949043 22.12770773605773 2 15.335780578163819 20.413442446841014 40.33081452990007 23.919962445095102 3 20.36033500521247 24.623114682774183 29.573868987792444 25.4426813242209 4 12.569517925916426 16.498371828717808 38.14953167808524 32.782578567280524 5 13.418647312916946 37.71567321485753 34.04284315205671 14.822836320168811 6 30.827220373859248 36.29605875567314 17.747714042659325 15.129006827808286 7 28.070547554541562 32.971668686724655 20.810681947811208 18.14710181092257 8 25.991408056717695 33.56533570102568 19.799871784648104 20.643384457608523 9 25.92138938693453 17.62447378458051 21.18019291297885 35.27394391550611 10-11 25.02527242763665 26.377776124383562 27.089725694473838 21.507225753505953 12-13 26.73356016216398 23.96038951248134 27.36663041592437 21.939419909430306 14-15 23.100951542662624 25.43612093632172 26.94275799260869 24.520169528406967 16-17 21.62564254833271 28.54938275042428 27.378968585882458 22.44600611536055 18-19 21.34433959637515 27.629915147681345 29.61035200514972 21.415393250793784 20-21 22.79546528926227 26.070244684932547 29.37488407264462 21.75940595316056 22-23 21.98257886347357 26.116653169935685 29.620941879595502 22.279826086995243 24-25 22.410310880713638 26.218594055834277 29.70486261882653 21.666232444625557 26-27 22.025182338470707 25.98791677086379 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22.002404848523707 52-53 22.42671359668017 27.68576927486673 27.73995696597908 22.147560162474022 54-55 22.001429716690353 27.123475471631746 28.054082562465503 22.821012249212398 56-57 22.6233314891727 27.156671649982023 27.689370621741876 22.530626239103405 58-59 23.143094283299625 26.86474254677385 27.755469528534523 22.236693641391998 60-61 22.42665475693778 27.171770380308335 28.67921789415985 21.722356968594028 62-63 22.092923329014216 27.36355823904748 28.51360076230169 22.029917669636614 64-65 21.89489540099199 27.963879741919285 27.92431852633693 22.216906330751797 66-67 22.161976477963783 27.398710713862723 27.4771427324709 22.96217007570259 68-69 22.038740817673702 27.325400827536463 27.977055176889536 22.658803177900303 70-71 22.685717237193806 27.106521503913665 27.640373542810355 22.567387716082173 72-73 22.440240642931787 26.865863410174086 28.347781009320606 22.346114937573525 74-75 21.940772685856892 28.153014137495227 27.539859399924648 22.366353776723233 76-77 22.240116259778965 27.295194240990078 27.56883049672507 22.895859002505883 78-79 22.363978430096328 27.40741417007375 27.720069554188292 22.50853784564163 80-81 22.71269796491544 27.369294561462997 27.38895495543236 22.529052518189204 82-83 22.516803110888176 27.299686813924456 27.914014203023036 22.26949587216433 84-85 22.307714571324887 27.676010713259995 27.646400081930167 22.369874633484947 86-87 22.274226494275585 27.80052207114193 27.46040774980659 22.464843684775893 88-89 22.176169840451763 27.71147961427018 26.996790779530006 23.115559765748056 90-91 22.32331883862111 27.291099255196954 27.346692870102192 23.038889036079745 92-93 22.71733036584611 27.51231146503651 27.490625296403614 22.27973287271377 94-95 22.30799186592356 27.33367637232481 27.703619655109996 22.654712106641632 96-97 21.774594506615855 27.39874382606028 28.186296174990655 22.640365492333213 98-99 21.986476068573626 27.727800483932818 27.257295400767717 23.02842804672584 100-101 21.98955222883558 27.14843984849396 27.517340583534295 23.344667339136166 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 301.0 1 243.5 2 338.0 3 488.5 4 425.0 5 379.0 6 590.5 7 1147.0 8 1858.5 9 2295.5 10 2151.0 11 1903.0 12 2014.0 13 2262.0 14 2508.5 15 2638.0 16 2800.0 17 3099.0 18 3412.0 19 3809.0 20 4249.5 21 4869.0 22 5808.5 23 7033.5 24 8594.0 25 10734.5 26 13853.0 27 17507.5 28 20422.0 29 23649.0 30 28052.0 31 32569.5 32 37775.0 33 42240.0 34 45986.0 35 50271.0 36 55505.0 37 59669.5 38 61023.0 39 62068.0 40 63704.5 41 65290.5 42 66881.0 43 69284.0 44 72342.0 45 75620.0 46 78324.0 47 88552.5 48 110098.0 49 109111.5 50 88198.5 51 77858.5 52 73387.0 53 71430.5 54 69639.0 55 64579.5 56 58221.5 57 52607.0 58 48935.0 59 44139.0 60 36924.0 61 30851.0 62 25518.0 63 19561.0 64 13911.5 65 10189.5 66 7776.5 67 6139.0 68 4728.0 69 3666.5 70 2914.5 71 2215.5 72 1733.5 73 1450.5 74 1119.0 75 821.5 76 610.5 77 379.0 78 257.5 79 189.0 80 116.0 81 69.5 82 41.5 83 29.0 84 21.0 85 16.0 86 14.0 87 8.5 88 6.0 89 8.0 90 7.0 91 3.5 92 1.0 93 2.0 94 4.0 95 2.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0749810098570641 2 0.029208754433630673 3 0.01447080059592983 4 0.019502186649283895 5 0.024444521622047626 6 0.029921163078353376 7 0.0775634911941839 8 0.055256195506304374 9 0.07008320042459555 10-11 0.060599260341724616 12-13 0.03964999363284774 14-15 0.0431229857758709 16-17 0.05461057517202443 18-19 0.049846342360441366 20-21 0.018812040774708782 22-23 0.013201822697517521 24-25 0.022574448929650534 26-27 0.024867514254851727 28-29 0.006166787330880867 30-31 0.0012912406685598927 32-33 0.010196348727593636 34-35 0.01139853831556319 36-37 0.004029561396712769 38-39 0.009461677312723352 40-41 0.004118612477303106 42-43 0.001825547152101917 44-45 2.003649313282592E-4 46-47 3.7846709250893406E-4 48-49 8.682480357557899E-4 50-51 0.0010018246566412961 52-53 2.003649313282592E-4 54-55 1.1131385073792177E-4 56-57 0.003317152751990069 58-59 0.004274451868336197 60-61 0.005298539295125076 62-63 0.003205838901252147 64-65 6.90145874575115E-4 66-67 3.562043223613497E-4 68-69 0.003539780453465913 70-71 0.0024489047162342794 72-73 0.02228503291773194 74-75 0.017097807473344787 76-77 0.02606970384282128 78-79 0.020414960225334854 80-81 0.012044158649843137 82-83 0.00500912328320648 84-85 0.0038737220056796777 86-87 0.0017587588416591643 88-89 0.001313503438707477 90-91 0.006255838411471204 92-93 0.010285399808183972 94-95 0.012801092834861005 96-97 0.012177735270728643 98-99 0.024444521622047626 100-101 0.019902916511940413 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2245902.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.854911248380716 #Duplication Level Percentage of deduplicated Percentage of total 1 81.0781551675137 49.34003936901487 2 11.598470480618555 14.116477834300115 3 3.2770191150636085 5.98268122219329 4 1.3430349479424346 3.2692109024204186 5 0.7023661405779525 2.1371214574369497 6 0.4181826446215676 1.5269080634435175 7 0.2820616144573899 1.201538416006561 8 0.18729797952300503 0.91184015366988 9 0.15647318693849793 0.856994571350422 >10 0.8562662667178158 9.622718086313865 >50 0.06561649590271183 2.7456234104085446 >100 0.03123732647098187 3.429457963252543 >500 0.00176244630086933 0.6793616283174504 >1k 0.0016155757757966471 1.9666719207335073 >5k 3.6717631268105616E-4 1.5919549589231439 >10k+ 7.343526253621124E-5 0.6214000422150385 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13905 0.6191276378043209 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9265 0.41252913083473813 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8636 0.38452256598907697 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7138 0.31782330662691427 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5421 0.24137295394010957 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5163 0.22988536454395608 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4090 0.18210945980724003 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4032 0.17952697847012025 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3240 0.14426275055634663 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3196 0.1423036267833592 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3100 0.138029174915023 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2656 0.1182598350239681 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2527 0.11251604032589133 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2450 0.10908757372316334 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2322 0.10338830456538176 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.2262770147584355E-5 0.0 20-21 0.0 0.0 0.0 4.452554029516871E-5 0.0 22-23 0.0 0.0 0.0 6.678831044275307E-5 0.0 24-25 0.0 0.0 0.0 8.905108059033742E-5 0.0 26-27 0.0 0.0 0.0 1.558393910330905E-4 2.2262770147584355E-5 28-29 0.0 0.0 0.0 4.4525540295168713E-4 4.452554029516871E-5 30-31 0.0 0.0 0.0 0.0010240874267888804 4.452554029516871E-5 32-33 0.0 0.0 0.0 0.0018255471521019173 4.452554029516871E-5 34-35 0.0 0.0 0.0 0.0027605834983004604 8.905108059033742E-5 36-37 0.0 0.0 0.0 0.004519342339959624 8.905108059033742E-5 38-39 0.0 0.0 0.0 0.005632480847338842 8.905108059033742E-5 40-41 0.0 0.0 0.0 0.007101823677079409 8.905108059033742E-5 42-43 0.0 0.0 0.0 0.009840144405232284 8.905108059033742E-5 44-45 0.0 0.0 0.0 0.01447080059592983 8.905108059033742E-5 46-47 4.452554029516871E-5 0.0 0.0 0.023220069263930485 8.905108059033742E-5 48-49 4.452554029516871E-5 0.0 0.0 0.041542329095392405 8.905108059033742E-5 50-51 4.452554029516871E-5 0.0 0.0 0.058239406706080674 8.905108059033742E-5 52-53 4.452554029516871E-5 0.0 0.0 0.0741350245914559 8.905108059033742E-5 54-55 4.452554029516871E-5 0.0 0.0 0.09630874365844992 8.905108059033742E-5 56-57 4.452554029516871E-5 0.0 0.0 0.11487589396153527 8.905108059033742E-5 58-59 4.452554029516871E-5 0.0 0.0 0.1387415835597457 8.905108059033742E-5 60-61 4.452554029516871E-5 0.0 0.0 0.1768999715927053 8.905108059033742E-5 62-63 4.452554029516871E-5 0.0 0.0 0.20512916413984225 2.2262770147584357E-4 64-65 4.452554029516871E-5 0.0 0.0 0.23778864794634852 2.2262770147584357E-4 66-67 4.452554029516871E-5 0.0 0.0 0.2952265949271161 2.2262770147584357E-4 68-69 4.452554029516871E-5 0.0 0.0 0.36364008759064287 2.2262770147584357E-4 70-71 4.452554029516871E-5 0.0 0.0 0.42305942111454553 2.2262770147584357E-4 72-73 4.452554029516871E-5 0.0 0.0 0.4870871480589981 2.8941601191859667E-4 74-75 4.452554029516871E-5 0.0 0.0 0.5429444383592872 3.11678782066181E-4 76-77 4.452554029516871E-5 0.0 0.0 0.5902750876930516 3.11678782066181E-4 78-79 4.452554029516871E-5 0.0 0.0 0.6576867556999371 3.11678782066181E-4 80-81 8.905108059033742E-5 0.0 0.0 0.7550195867851759 3.339415522137653E-4 82-83 1.7810216118067484E-4 0.0 0.0 0.8658659193499983 3.562043223613497E-4 84-85 1.7810216118067484E-4 0.0 0.0 0.9926301325703437 3.562043223613497E-4 86-87 1.7810216118067484E-4 0.0 0.0 1.1974698806982673 3.562043223613497E-4 88-89 1.7810216118067484E-4 0.0 0.0 1.453358160774602 3.562043223613497E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9820 0.0 24.685722 1 TACCTGG 1690 0.0 23.622797 2 GTACCTG 1795 0.0 23.567455 1 TACATGG 9850 0.0 23.49803 2 ACATGGG 9805 0.0 22.39657 3 GTATAGG 785 0.0 21.192663 1 GAGTACT 9530 0.0 21.071093 12-13 GTACTTT 10140 0.0 19.70866 14-15 AGTACTT 9805 0.0 19.340982 12-13 GGACCGT 200 1.2774179E-5 19.014116 6 CATGGGG 4690 0.0 18.952883 4 AGAGTAC 15845 0.0 18.180113 10-11 TAGCCCT 1650 0.0 17.861347 4 TAAGGTG 1015 0.0 17.328522 5 CATGGGA 5090 0.0 17.276686 4 ACTTTTT 12095 0.0 16.93465 16-17 GTGTAGG 1015 0.0 16.85868 1 TACTTTT 11685 0.0 16.309654 14-15 AGTACAT 7505 0.0 16.148352 2 GTGTAGC 1365 0.0 16.018148 1 >>END_MODULE