FastQCFastQC Report
Thu 26 May 2016
SRR936728_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936728_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2245902
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT162230.722337840208522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95100.42343788820705447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86380.38461161706966734No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT82500.3673357074351419No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53540.23838974274033325No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT49280.21942186257459143No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC48730.21697295785835713No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT43300.19279558947808054No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC38760.17258099418407394No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC37750.16808391461426186No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT31810.14163574367893167No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG30680.1366043576255776No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA29180.1299255265813023No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA27160.12093136744167822No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26090.11616713463009516No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC24380.1085532672396213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG104150.022.5827051
TACATGG104300.022.04422
TACCTGG16950.021.8604072
GAGTACT108300.021.77248812-13
GTACCTG18300.021.290841
GTATAGG6600.020.8777681
ACATGGG105200.020.7700123
GTACTTT114400.020.40397514-15
AGTACTT113250.019.39504612-13
AGAGTAC179350.018.4564710-11
ACTTTTT131600.018.151816-17
ACACCGT5250.018.0916546
CATGGGG49400.016.9204794
TAGACCG1700.001790117516.762134
TACTTTT130600.016.70935214-15
CCTATAC5450.016.5596733
TACACTG18350.016.3053995
GTACACG4106.1118044E-1016.2246071
GTGTAGC14200.016.0613821
ACCGTGC3854.667527E-916.0357868
Produced by FastQC (version 0.11.5)