FastQCFastQC Report
Thu 26 May 2016
SRR936727_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936727_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3448808
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT84310.24446127473608273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67450.1955748188939483No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT52110.1510956829142127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51750.15005184399943403No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42370.1228540411643675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG116200.026.2471491
TACATGG121950.024.5424692
ACATGGG119250.023.9428393
GTACGCT1601.858898E-623.7536053
TAGATCG1903.2851676E-722.5040685
GAGTACT80300.021.68465812-13
GTGTAGC24950.021.515981
TAGCCCT25300.020.8433884
GTATCAA204550.020.786281
CATGGGG70650.020.576674
AGAGTAC139850.020.41811610-11
GTACTTT88850.019.59994314-15
AGTACTT88500.019.35335212-13
GTAGCCC21850.019.3507633
TCAACGC224300.018.6812174
ATCAACG224950.018.6480873
CAACGCA225400.018.5692395
AACGCAG228100.018.2874856
TATCAAC231600.018.1536642
GAGTACA90000.016.7856371
Produced by FastQC (version 0.11.5)