##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936727_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3448808 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.34945204256079 31.0 31.0 34.0 28.0 34.0 2 31.434304838077388 31.0 31.0 34.0 28.0 34.0 3 31.454389458618746 31.0 31.0 34.0 28.0 34.0 4 35.08142842396561 37.0 35.0 37.0 32.0 37.0 5 35.081457419491024 37.0 35.0 37.0 32.0 37.0 6 35.07781239199167 37.0 35.0 37.0 32.0 37.0 7 34.98878511068172 37.0 35.0 37.0 32.0 37.0 8 34.94350424842438 37.0 35.0 37.0 32.0 37.0 9 36.43901922055388 39.0 37.0 39.0 32.0 39.0 10-11 36.31078302996281 39.0 35.0 39.0 32.0 39.0 12-13 36.203258778105365 39.0 35.0 39.0 32.0 39.0 14-15 37.29724269950661 40.0 37.0 41.0 31.5 41.0 16-17 37.19045667372611 39.0 36.0 41.0 31.0 41.0 18-19 37.133929606983045 39.0 36.0 41.0 31.0 41.0 20-21 36.981967392791944 39.0 36.0 41.0 31.0 41.0 22-23 37.091411873319714 39.0 36.0 41.0 31.0 41.0 24-25 37.130238766553546 39.0 36.0 41.0 31.0 41.0 26-27 37.04407725799754 39.0 36.0 41.0 31.0 41.0 28-29 36.904472646781144 39.0 36.0 41.0 30.0 41.0 30-31 36.72361972600389 39.0 36.0 41.0 30.0 41.0 32-33 36.5461788536793 39.0 35.5 41.0 30.0 41.0 34-35 36.366907493835555 39.0 35.0 40.5 30.0 41.0 36-37 36.169306467625916 39.0 35.0 40.0 29.5 41.0 38-39 35.980013094379274 38.5 35.0 40.0 29.0 41.0 40-41 35.780145922881175 38.0 35.0 40.0 27.5 41.0 42-43 35.555241695101614 38.0 35.0 40.0 27.0 41.0 44-45 35.32650382392989 38.0 34.0 40.0 26.5 41.0 46-47 35.22179605243319 38.0 34.0 40.0 26.0 41.0 48-49 35.10911103198555 38.0 34.0 40.0 26.0 41.0 50-51 34.14820482903078 37.5 33.0 39.5 24.5 40.5 52-53 34.26657282748126 37.5 33.5 39.5 24.0 40.5 54-55 34.7133727073238 38.0 34.0 40.0 25.0 41.0 56-57 34.61043337292189 38.0 34.0 40.0 25.0 41.0 58-59 34.37476803579672 38.0 34.0 40.0 24.0 41.0 60-61 34.07934640026351 37.5 33.0 40.0 23.0 41.0 62-63 33.70986178412947 37.0 33.0 40.0 22.5 41.0 64-65 33.35677341272695 36.5 32.5 39.5 22.0 41.0 66-67 32.89593389948063 36.0 32.0 39.0 21.0 41.0 68-69 32.49936238839622 35.5 31.5 39.0 20.0 40.0 70-71 32.09362089742311 35.0 31.0 38.0 19.5 40.0 72-73 31.689601015771245 35.0 31.0 37.5 18.0 39.5 74-75 31.251508927142364 35.0 31.0 37.0 17.0 39.0 76-77 30.784885966397667 34.5 30.0 36.0 12.5 39.0 78-79 30.289993673176355 34.0 30.0 36.0 8.0 38.5 80-81 29.832241168542872 34.0 29.0 35.5 7.0 37.0 82-83 29.418310036395184 34.0 29.0 35.0 4.0 37.0 84-85 29.04523548426007 34.0 29.0 35.0 2.0 36.0 86-87 28.687218598425893 34.0 29.0 35.0 2.0 36.0 88-89 28.283756735660553 34.0 27.5 35.0 2.0 35.5 90-91 27.869675116735984 33.5 27.0 35.0 2.0 35.0 92-93 27.41228317146098 33.0 26.0 35.0 2.0 35.0 94-95 26.903786177717052 33.0 25.0 35.0 2.0 35.0 96-97 26.298453987580636 33.0 24.5 35.0 2.0 35.0 98-99 25.526687336610216 32.5 22.0 35.0 2.0 35.0 100-101 22.64232120199211 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4622806408717146 1101 2 0.4375657123130843 1101 3 0.49706521081028043 1101 4 0.488984255720176 1101 5 0.38918493509503094 1101 6 0.505630850110478 1101 7 0.5298911015776255 1101 8 0.5774013464065746 1101 9 0.5779461457250932 1101 10-11 0.7549104407745375 1101 12-13 0.7506905383105291 1101 14-15 0.8074888371800526 1101 16-17 0.955299901451589 1101 18-19 0.7231117038453831 1101 20-21 0.8809113713306758 1101 22-23 0.7635037510683702 1101 24-25 0.7403284422178515 1101 26-27 0.750889790614913 1101 28-29 0.885979104422276 1101 30-31 0.8495647459540052 1101 32-33 0.7441797186562411 1101 34-35 0.6152552246233967 1101 36-37 0.5724033352348172 1101 38-39 0.5351891893474203 1101 40-41 0.6160721418417481 1101 42-43 0.5609267718991546 1101 44-45 0.6494141142338421 1101 46-47 0.630510392965725 1101 48-49 0.8444802160266889 1101 50-51 0.6404573569186596 1101 52-53 0.6757699670802779 1101 54-55 0.7307958480264531 1101 56-57 0.6161047009731035 1101 58-59 0.703540027624193 1101 60-61 0.6943602660144279 1101 62-63 0.7258653083111639 1101 64-65 0.5300164631292859 1101 66-67 0.7166858600490258 1101 68-69 0.5391606947184471 1101 70-71 0.5055739102081631 1101 72-73 0.5244782882664687 1101 74-75 0.5805120516637494 1101 76-77 0.5425160605852177 1101 78-79 0.552952287966221 1101 80-81 0.5056562192752487 1101 82-83 0.4899645355076139 1101 84-85 0.5462691958768531 1101 86-87 0.5459830068169857 1101 88-89 0.5384268146073303 1101 90-91 0.48927936317151577 1101 92-93 0.4357507115767021 1101 94-95 0.5828087634511157 1101 96-97 0.3362363117430398 1101 98-99 0.34088322030910945 1101 100-101 0.4572419331524813 1102 1 0.31496791539162317 1102 2 0.4005221188731376 1102 3 0.3991498979578232 1102 4 0.3329249567905279 1102 5 0.44969009763987344 1102 6 0.5003143127875376 1102 7 0.4170299288446273 1102 8 0.5258612349145295 1102 9 0.48200061698439356 1102 10-11 0.5903426529629741 1102 12-13 0.684257941452131 1102 14-15 0.5844342837582275 1102 16-17 0.4238414918689486 1102 18-19 0.47652238198492114 1102 20-21 0.4189935114401777 1102 22-23 0.43611375547526876 1102 24-25 0.4800150544580788 1102 26-27 0.17514170711787358 1102 28-29 0.10142481740619047 1102 30-31 0.3743022616084559 1102 32-33 0.24307955009858517 1102 34-35 0.15856504827276297 1102 36-37 -0.04377209916837188 1102 38-39 0.03144388568222922 1102 40-41 -0.07542217027124565 1102 42-43 -0.07929974767171188 1102 44-45 -0.03328650547072698 1102 46-47 0.057312833192490586 1102 48-49 0.15364068315795265 1102 50-51 0.09945035567626803 1102 52-53 0.11701259423922039 1102 54-55 -0.00998813177650959 1102 56-57 0.04450585572993759 1102 58-59 0.022512504425733937 1102 60-61 0.0028289185242442727 1102 62-63 0.10919543606143378 1102 64-65 0.0691883147487573 1102 66-67 0.06680512018800755 1102 68-69 0.1115974731510363 1102 70-71 0.016270225094764612 1102 72-73 -0.19668378850319712 1102 74-75 -0.11542936895107658 1102 76-77 -0.08352145905287145 1102 78-79 -0.06634161856270993 1102 80-81 0.004368570791392301 1102 82-83 -0.04939118586683833 1102 84-85 -0.4104361680906514 1102 86-87 -0.24030061445862216 1102 88-89 -0.09394285198713348 1102 90-91 -0.19308977434612729 1102 92-93 -0.2683233666469924 1102 94-95 -0.48284665068532107 1102 96-97 -0.4218289974992473 1102 98-99 -0.33778807484603846 1102 100-101 0.06863852788960045 1103 1 0.3509642475397321 1103 2 0.14447669294344223 1103 3 0.2745505317254455 1103 4 0.414449937032181 1103 5 0.43022056983890167 1103 6 0.4030021128492294 1103 7 0.5649566794048937 1103 8 0.46864507545654277 1103 9 0.3154222430007465 1103 10-11 0.4620711345721986 1103 12-13 0.20474809607076594 1103 14-15 0.4814084340395155 1103 16-17 0.5956263283036307 1103 18-19 0.6326103652928197 1103 20-21 0.6647008248862747 1103 22-23 0.4629863273883572 1103 24-25 0.4625202939197237 1103 26-27 0.38172627348368593 1103 28-29 0.46968841354917856 1103 30-31 0.32967880413041684 1103 32-33 0.2880623084742382 1103 34-35 0.5470225076991468 1103 36-37 0.5399327496461908 1103 38-39 0.3759087457128629 1103 40-41 0.3080711969811105 1103 42-43 0.42182978870417287 1103 44-45 0.4242847583138314 1103 46-47 0.44682751743986415 1103 48-49 0.40958195726985736 1103 50-51 0.4355356453711181 1103 52-53 0.5534715373486634 1103 54-55 0.5294977894900867 1103 56-57 0.5116531700739131 1103 58-59 0.41961081343328743 1103 60-61 0.4527160960294978 1103 62-63 0.5172122408886111 1103 64-65 0.5135308947506303 1103 66-67 0.6132719885950593 1103 68-69 0.48855047222626524 1103 70-71 0.416525294878916 1103 72-73 0.2768933070511963 1103 74-75 0.23841626079289924 1103 76-77 0.3594158491164059 1103 78-79 0.4341009122941344 1103 80-81 0.3669984837912317 1103 82-83 0.14990131983192612 1103 84-85 0.25342510912964755 1103 86-87 0.31879323605817333 1103 88-89 0.09378102486131823 1103 90-91 0.13777124186941236 1103 92-93 0.15496456503331757 1103 94-95 0.24715517860488134 1103 96-97 0.3148953970279642 1103 98-99 0.4547102433232162 1103 100-101 0.4047855767150601 1104 1 0.29090885122638355 1104 2 0.32082883683181507 1104 3 0.18878057068707577 1104 4 0.21150656400481438 1104 5 0.29639719219517957 1104 6 0.30321883760878166 1104 7 0.3581920482642218 1104 8 0.4540025603396316 1104 9 0.47765398646432544 1104 10-11 0.4952170947583241 1104 12-13 0.4408743268687587 1104 14-15 0.36886719474674834 1104 16-17 0.37679285792212625 1104 18-19 0.24940037531342085 1104 20-21 0.14329190310582618 1104 22-23 0.27934150076261943 1104 24-25 0.35640173116109963 1104 26-27 0.13736383600150504 1104 28-29 0.19045831355394682 1104 30-31 0.3119282917256214 1104 32-33 0.31154054666728825 1104 34-35 0.47383711980843657 1104 36-37 0.30002937138102936 1104 38-39 0.24189866074299005 1104 40-41 0.1477725540354342 1104 42-43 0.390092872365706 1104 44-45 0.3761625401975053 1104 46-47 0.18116435410174603 1104 48-49 0.441611280029953 1104 50-51 0.341987229636878 1104 52-53 0.38300996798619735 1104 54-55 0.33082982003351447 1104 56-57 0.2872409513694407 1104 58-59 0.2493201381464587 1104 60-61 0.2783605830864957 1104 62-63 0.38839361831706043 1104 64-65 0.3815995586585714 1104 66-67 0.36236009114927015 1104 68-69 0.288493710979715 1104 70-71 0.30799951151268345 1104 72-73 0.2558367155890231 1104 74-75 0.2806184972858894 1104 76-77 0.24233204554694154 1104 78-79 0.2230189863400973 1104 80-81 0.3900913780377593 1104 82-83 0.15327703267661263 1104 84-85 0.1760428290697682 1104 86-87 0.17447929254421268 1104 88-89 0.1469945547894227 1104 90-91 0.08080164880815488 1104 92-93 -0.14511738644386085 1104 94-95 -0.06494512068169556 1104 96-97 0.06352602501930349 1104 98-99 0.1367601510515115 1104 100-101 0.19130170123691315 1105 1 0.262385197332204 1105 2 0.1348533587532721 1105 3 0.29624343371731854 1105 4 0.29809901102137104 1105 5 0.2585486692892687 1105 6 0.3355657927572864 1105 7 0.4289494819919355 1105 8 0.45578506250215867 1105 9 0.5060541017991511 1105 10-11 0.5162612368711024 1105 12-13 0.20190394354936103 1105 14-15 0.3315197027363368 1105 16-17 0.2766135219177528 1105 18-19 0.2907193786564406 1105 20-21 0.2640080768850126 1105 22-23 -0.15143498251443788 1105 24-25 -0.012330104508492923 1105 26-27 -0.16208591070824951 1105 28-29 -0.18921808539356277 1105 30-31 -0.16020730873330535 1105 32-33 -0.21610778103804051 1105 34-35 -0.04896720548886435 1105 36-37 -0.08600642825970084 1105 38-39 -0.1223957398476756 1105 40-41 -0.08753413888317851 1105 42-43 -0.14466302436861156 1105 44-45 0.00964902997722561 1105 46-47 0.06474162783255366 1105 48-49 -0.044685350045654104 1105 50-51 0.08699109624901524 1105 52-53 0.19193188896253588 1105 54-55 0.020178781477177665 1105 56-57 -0.014735786223312175 1105 58-59 0.05517410447014015 1105 60-61 0.11566838350898223 1105 62-63 0.09204801919727146 1105 64-65 0.09606557409365735 1105 66-67 0.18651000953418873 1105 68-69 0.19706968779693312 1105 70-71 0.04998778258813985 1105 72-73 -0.05127561884336984 1105 74-75 0.20773219498558504 1105 76-77 0.01865915007576291 1105 78-79 0.10430693614290121 1105 80-81 0.20327465545300427 1105 82-83 0.07758529516227242 1105 84-85 0.02689843010778148 1105 86-87 -0.2073068530406701 1105 88-89 -0.1398376704368154 1105 90-91 -0.20705290625397055 1105 92-93 -0.41490291916167266 1105 94-95 -0.09582228064602916 1105 96-97 -0.21681294596723433 1105 98-99 -0.07690666400619861 1105 100-101 -0.06279346929817464 1106 1 0.3324153376912484 1106 2 0.19863177432067403 1106 3 0.3140156868291406 1106 4 0.36913486988962063 1106 5 0.21198253447835214 1106 6 0.38489975321486725 1106 7 0.43468682892042665 1106 8 0.44895308780470344 1106 9 0.4955968850309347 1106 10-11 0.5252914060266676 1106 12-13 0.4551064766833619 1106 14-15 0.5016732481880481 1106 16-17 0.511820508913722 1106 18-19 0.32616903633779515 1106 20-21 0.1519665714988605 1106 22-23 0.27039326063379576 1106 24-25 0.22689279736496815 1106 26-27 0.2797931702987668 1106 28-29 0.33054580712822457 1106 30-31 0.3843556680590936 1106 32-33 0.277717524865011 1106 34-35 0.21010723831832934 1106 36-37 0.1575376316178776 1106 38-39 0.1163459001622158 1106 40-41 0.15571835621541652 1106 42-43 0.09230972932274284 1106 44-45 0.06895666561484148 1106 46-47 0.013946963827962122 1106 48-49 0.08702045989250706 1106 50-51 -0.043032396913005755 1106 52-53 -0.04795051324327204 1106 54-55 0.06191651955841593 1106 56-57 0.14884939268706887 1106 58-59 0.10930418555633992 1106 60-61 0.1423215438072205 1106 62-63 0.20706018572754914 1106 64-65 0.19662506650696798 1106 66-67 0.2329926344331028 1106 68-69 0.24750103569010662 1106 70-71 0.14665218552826076 1106 72-73 -0.0978277753428678 1106 74-75 0.21678684118432656 1106 76-77 0.1079212272168455 1106 78-79 -0.03582635575543591 1106 80-81 0.23262886946395156 1106 82-83 0.11992344071428818 1106 84-85 0.13974395836796916 1106 86-87 0.0836008853045378 1106 88-89 0.16218846480604654 1106 90-91 0.062147414347297314 1106 92-93 0.11113087595587601 1106 94-95 0.1582070726080964 1106 96-97 0.24162443479717055 1106 98-99 0.30297164669386945 1106 100-101 0.15761546530252346 1107 1 0.20488651326019536 1107 2 0.2779484213914465 1107 3 0.22076180407209023 1107 4 0.36519449475650134 1107 5 0.4046335203262217 1107 6 0.3685187351722803 1107 7 0.4382830960565727 1107 8 0.3779305515010023 1107 9 0.4841654819444301 1107 10-11 0.42557484883025154 1107 12-13 0.36229556371991123 1107 14-15 0.08116598129197428 1107 16-17 0.25610610008387624 1107 18-19 0.32037407915122884 1107 20-21 0.3104982337436226 1107 22-23 0.3815011160222568 1107 24-25 0.31917215888765327 1107 26-27 0.34684766124669153 1107 28-29 0.35914068921429276 1107 30-31 0.3998558558103298 1107 32-33 0.147812631636981 1107 34-35 0.19831987736196766 1107 36-37 0.26007947551965316 1107 38-39 0.16795692885429503 1107 40-41 0.24889916988616534 1107 42-43 0.2607322804003118 1107 44-45 0.2488478930154443 1107 46-47 0.26974952340050606 1107 48-49 0.4620922054865062 1107 50-51 0.2904419858163436 1107 52-53 0.4052017068381062 1107 54-55 0.45165788454065847 1107 56-57 0.29667594132695285 1107 58-59 0.3716674195559335 1107 60-61 0.39388187966647337 1107 62-63 0.5900878128597569 1107 64-65 0.46997756482952013 1107 66-67 0.5035865439062306 1107 68-69 0.5393078181264457 1107 70-71 0.42058363478309957 1107 72-73 0.48839697827211737 1107 74-75 0.6754477439907447 1107 76-77 0.590346696133242 1107 78-79 0.44224882476275695 1107 80-81 0.45039961597516154 1107 82-83 0.6054366479362443 1107 84-85 0.45622888594524014 1107 86-87 0.4658103171877741 1107 88-89 0.3400159983704292 1107 90-91 0.2708900351082768 1107 92-93 0.38671093492822806 1107 94-95 0.4251967739912992 1107 96-97 0.5050546190396084 1107 98-99 0.6619563742083514 1107 100-101 0.515282117605711 1108 1 0.1839417312308136 1108 2 0.14902286004449294 1108 3 0.15495492214087747 1108 4 0.22644997670618494 1108 5 0.10993599242914343 1108 6 0.11706699120379227 1108 7 0.1308798140107541 1108 8 0.0894019294999282 1108 9 0.17756891743004388 1108 10-11 0.12862617130902265 1108 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#Quality Count 2 7400.0 3 3083.0 4 3305.0 5 3661.0 6 4051.0 7 4885.0 8 6022.0 9 7588.0 10 9653.0 11 11573.0 12 13956.0 13 16151.0 14 18558.0 15 21202.0 16 24071.0 17 28515.0 18 24882.0 19 21716.0 20 22905.0 21 25102.0 22 27683.0 23 31695.0 24 36641.0 25 42588.0 26 49067.0 27 58029.0 28 69111.0 29 82812.0 30 100316.0 31 122736.0 32 152923.0 33 193196.0 34 253237.0 35 341410.0 36 474955.0 37 614526.0 38 460169.0 39 59421.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.72003476424086 18.287939435750967 14.384125141987333 23.607900658020846 2 15.512246556226977 19.61255632325503 40.10639143161549 24.7688056889025 3 19.77102532849969 23.30105393075893 29.219456728171817 27.708464012569568 4 12.97277278833282 16.03367353330812 37.09230524730814 33.901248431050924 5 14.32440647801274 36.74151411191847 34.012786406336474 14.921293003732316 6 32.92474061542157 35.232121856649954 17.31763014483067 14.525507383097807 7 29.3523121270792 31.840379096456235 20.357963371446154 18.44934540501841 8 26.49784467444767 34.196749334608675 19.614463114683968 19.69094287625969 9 26.098735352129015 17.09977955679313 20.332927253741733 36.46855783733612 10-11 25.350344466897575 25.95630337220917 27.27409720027551 21.41925496061775 12-13 26.338236633441092 23.182827273238807 27.521096391671545 22.957839701648556 14-15 22.975905480477913 24.84541037020881 25.936369400186436 26.242314749126844 16-17 22.805710324584734 28.057219262194046 26.385800759326987 22.751269653894234 18-19 22.565617816973273 27.054852276621144 28.22393054787602 22.155599358529564 20-21 23.37674337643876 26.006295374930193 28.166218205818144 22.450743042812903 22-23 22.867554794617874 25.959328112906448 28.206950690136257 22.966166402339418 24-25 23.00910834382519 26.150779451771182 28.193502142542737 22.646610061860883 26-27 22.776854260921205 26.30825513885663 28.299006632213835 22.615883968008333 28-29 22.430331313261938 26.785984306740406 28.096583005333297 22.68710137466436 30-31 22.72181286786256 26.174365674719937 28.11321348319168 22.990607974225828 32-33 22.501907936552122 26.33104670907041 28.15601121797102 23.011034136406444 34-35 23.26174724716482 26.332431379189565 27.772175197923453 22.633646175722163 36-37 23.104042885653815 26.054011845862863 28.025509749915837 22.81643551856748 38-39 22.792087639593582 26.421299658302082 27.986913733780078 22.799698968324257 40-41 23.032782440796957 26.314752489802878 27.897289089115034 22.755175980285127 42-43 22.85804008990057 26.393271781619674 27.547620131533662 23.201067996946094 44-45 23.06356153174452 26.271772133785458 27.564932386577727 23.099733947892293 46-47 23.25789456820884 26.58660839291117 27.20200001188826 22.953497026991723 48-49 23.210661756710714 26.603475146972478 27.172387296938766 23.01347579937804 50-51 22.793191782782166 26.739927430320915 27.39356778111544 23.07331300578148 52-53 23.242664099521342 26.803824331289334 26.899671833578232 23.053839735611096 54-55 22.935113284725674 26.438210960950187 27.206761750705027 23.419914003619112 56-57 23.067734773068466 26.738595431866173 26.98460978620716 23.2090600088582 58-59 23.478390725605927 26.510590422007546 26.90183217417906 23.109186678207465 60-61 23.16229804481253 26.531791622580297 27.50795004411778 22.797960288489392 62-63 22.92498358845648 26.620349966017088 27.409379864021954 23.04528658150448 64-65 22.745909066526327 26.88574381992499 27.18740078027479 23.18094633327389 66-67 22.88768741493003 26.80905133582415 26.846171458134954 23.45708979111086 68-69 22.838295823913736 26.563053636263035 27.25238902545804 23.346261514365192 70-71 23.050109507616796 26.484365265596765 27.06695536043374 23.398569866352695 72-73 23.009904332264494 26.39613443072782 27.314241932738238 23.27971930426945 74-75 22.786194442675985 26.988492371778577 26.9521498980569 23.273163287488547 76-77 22.931723232732498 26.62333208281387 26.92663259859152 23.518312085862107 78-79 23.051650588414144 26.66203350728714 26.96543726928491 23.320878635013802 80-81 23.09756661304474 26.772392837518886 26.879849496835572 23.250191052600805 82-83 22.99574267609309 26.7166620468661 27.069710542297866 23.217884734742945 84-85 22.988461745928724 26.730013090956334 26.947983024097915 23.333542139017023 86-87 22.973158933514842 26.759602051024462 26.922864433350185 23.344374582110508 88-89 22.918114643356542 26.81633736782598 26.63627951787941 23.629268470938065 90-91 23.017757520891244 26.5971805051607 26.8598203276174 23.52524164633066 92-93 23.190809866186036 26.53935654968005 27.06317375919693 23.206659824936988 94-95 22.940142218453598 26.523152910212023 27.036310335617802 23.500394535716573 96-97 22.70665701902316 26.500457489708296 27.269185204304318 23.523700286964225 98-99 22.74764787768311 26.6475082363989 26.795315932059516 23.80952795385847 100-101 22.78042545659765 26.445509319104055 26.836974073058528 23.937091151239766 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 139.0 1 111.5 2 120.5 3 172.0 4 165.5 5 175.5 6 298.0 7 597.0 8 981.0 9 1272.5 10 1301.5 11 1259.0 12 1381.0 13 1578.0 14 1784.5 15 1960.0 16 2146.0 17 2347.0 18 2585.5 19 2849.0 20 3337.5 21 4067.0 22 4873.5 23 6038.0 24 7559.5 25 9533.0 26 12340.0 27 16113.0 28 20074.0 29 24906.5 30 31324.0 31 38440.5 32 46483.0 33 53008.5 34 59409.0 35 67077.0 36 75468.0 37 82850.5 38 87482.5 39 92100.0 40 97417.0 41 104960.0 42 112496.0 43 118588.5 44 123217.5 45 127961.0 46 131681.5 47 140236.0 48 156341.0 49 155332.0 50 140897.0 51 132015.5 52 125987.0 53 123132.0 54 120535.5 55 113236.0 56 104103.5 57 94863.0 58 86285.0 59 75866.0 60 63139.5 61 51456.0 62 41880.5 63 33315.5 64 25539.0 65 20112.5 66 15500.0 67 12289.5 68 9174.5 69 6098.5 70 4542.0 71 3535.0 72 2856.5 73 2412.0 74 1870.5 75 1413.5 76 1006.0 77 622.0 78 415.0 79 266.0 80 165.5 81 114.0 82 78.0 83 54.5 84 40.0 85 28.0 86 18.0 87 16.0 88 16.5 89 13.5 90 9.0 91 6.0 92 4.5 93 2.5 94 1.0 95 1.5 96 2.5 97 2.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01267104460439665 2 0.011888165418312646 3 0.013888856671638434 4 0.018557136262731935 5 0.031431149545002215 6 0.04668279591093503 7 0.005422163251766987 8 0.024269254768604108 9 0.03502659469590653 10-11 0.03999932730381048 12-13 0.030793247985970804 14-15 0.02812565964820309 16-17 0.029517444867907982 18-19 0.02297895388783603 20-21 0.053148798077480686 22-23 0.012497071451933538 24-25 0.0029575435918729022 26-27 0.0010148433893681526 28-29 7.248881352629662E-5 30-31 0.002450121897188826 32-33 0.0016237494229890443 34-35 0.0 36-37 2.609597286946678E-4 38-39 4.349328811577797E-5 40-41 0.0011888165418312646 42-43 0.0031605122697465327 44-45 0.0013772874569996359 46-47 8.263724741997815E-4 48-49 9.568523385471155E-4 50-51 0.0035519518627885344 52-53 0.002914050303757124 54-55 0.0050597191841355044 56-57 7.393858979682256E-4 58-59 0.005016225896019726 60-61 0.0024646196598940855 62-63 4.6392840656829835E-4 64-65 6.66897084441929E-4 66-67 0.015759068060616884 68-69 0.041449103574336406 70-71 0.05182950167130208 72-73 0.015048677688059179 74-75 0.030488794969160356 76-77 0.026313439310045674 78-79 0.055555426686553736 80-81 0.08024511657361036 82-83 0.048987940181071256 84-85 0.018151198906984675 86-87 0.019644468465626386 88-89 0.021732146295183725 90-91 0.03801313381318995 92-93 0.024631698836235595 94-95 0.01307698196014391 96-97 0.01864412283896349 98-99 0.0510756180106286 100-101 0.06938629230737113 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3448808.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.33494935012138 #Duplication Level Percentage of deduplicated Percentage of total 1 73.29004252589827 38.35630663461128 2 14.280187312543108 14.94705759424379 3 5.3068045456063295 8.331940413159034 4 2.429949510662796 5.0868513825555866 5 1.337320297950638 3.499429502906793 6 0.796681164085276 2.50165610223592 7 0.5009768292965783 1.835301789077458 8 0.36418011431703906 1.5247478269682921 9 0.2592445997691832 1.2210797698391422 >10 1.2928947563770044 12.116630745264208 >50 0.09037869137374814 3.280990931239544 >100 0.04722619904657528 4.384810098359672 >500 0.0026681857806007355 0.9746536941472567 >1k 0.0012229184786828563 1.1949057018911065 >5k 2.2234881430597387E-4 0.7436378135010094 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8431 0.24446127473608273 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6745 0.1955748188939483 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5211 0.1510956829142127 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5175 0.15005184399943403 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4237 0.1228540411643675 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 2.899552541051865E-5 0.0 0.0 0.0 14-15 0.0 8.698657623155594E-5 0.0 0.0 0.0 16-17 0.0 8.698657623155594E-5 0.0 0.0 0.0 18-19 0.0 8.698657623155594E-5 0.0 0.0 0.0 20-21 0.0 8.698657623155594E-5 0.0 0.0 0.0 22-23 0.0 8.698657623155594E-5 0.0 7.248881352629662E-5 1.4497762705259325E-5 24-25 0.0 8.698657623155594E-5 0.0 2.0296867787363053E-4 2.899552541051865E-5 26-27 0.0 8.698657623155594E-5 0.0 3.4794630492622383E-4 2.899552541051865E-5 28-29 0.0 8.698657623155594E-5 0.0 6.523993217366695E-4 2.899552541051865E-5 30-31 0.0 8.698657623155594E-5 0.0 0.0015367628467574885 2.899552541051865E-5 32-33 1.4497762705259325E-5 8.698657623155594E-5 0.0 0.0025806017615361597 2.899552541051865E-5 34-35 2.899552541051865E-5 8.698657623155594E-5 0.0 0.0038129115914832023 2.899552541051865E-5 36-37 2.899552541051865E-5 8.698657623155594E-5 0.0 0.005219194573893356 2.899552541051865E-5 38-39 2.899552541051865E-5 8.698657623155594E-5 0.0 0.007756303047313739 2.899552541051865E-5 40-41 2.899552541051865E-5 8.698657623155594E-5 0.0 0.012960999858501836 2.899552541051865E-5 42-43 2.899552541051865E-5 8.698657623155594E-5 0.0 0.017948230229111045 2.899552541051865E-5 44-45 2.899552541051865E-5 8.698657623155594E-5 2.899552541051865E-5 0.024182268192372555 2.899552541051865E-5 46-47 2.899552541051865E-5 8.698657623155594E-5 2.899552541051865E-5 0.036606850830779795 2.899552541051865E-5 48-49 2.899552541051865E-5 8.698657623155594E-5 2.899552541051865E-5 0.04521852187770384 2.899552541051865E-5 50-51 2.899552541051865E-5 8.698657623155594E-5 2.899552541051865E-5 0.056526776787806106 2.899552541051865E-5 52-53 2.899552541051865E-5 8.698657623155594E-5 2.899552541051865E-5 0.07111152606929699 2.899552541051865E-5 54-55 2.899552541051865E-5 8.698657623155594E-5 2.899552541051865E-5 0.09113293636526013 2.899552541051865E-5 56-57 2.899552541051865E-5 8.698657623155594E-5 2.899552541051865E-5 0.13481469539620644 2.899552541051865E-5 58-59 2.899552541051865E-5 8.698657623155594E-5 2.899552541051865E-5 0.17983024859603666 2.899552541051865E-5 60-61 4.349328811577798E-5 8.698657623155594E-5 2.899552541051865E-5 0.216132646410006 2.899552541051865E-5 62-63 5.79910508210373E-5 8.698657623155594E-5 2.899552541051865E-5 0.25882855757699474 2.899552541051865E-5 64-65 5.79910508210373E-5 8.698657623155594E-5 2.899552541051865E-5 0.30440952352233003 2.899552541051865E-5 66-67 5.79910508210373E-5 8.698657623155594E-5 2.899552541051865E-5 0.3796674097253312 2.899552541051865E-5 68-69 5.79910508210373E-5 8.698657623155594E-5 2.899552541051865E-5 0.47214863802218043 2.899552541051865E-5 70-71 5.79910508210373E-5 8.698657623155594E-5 2.899552541051865E-5 0.5241956061340614 2.899552541051865E-5 72-73 5.79910508210373E-5 8.698657623155594E-5 2.899552541051865E-5 0.5788811670582996 2.899552541051865E-5 74-75 5.79910508210373E-5 8.698657623155594E-5 2.899552541051865E-5 0.6750158315568742 2.899552541051865E-5 76-77 5.79910508210373E-5 8.698657623155594E-5 2.899552541051865E-5 0.7881418739460126 2.899552541051865E-5 78-79 5.79910508210373E-5 8.698657623155594E-5 2.899552541051865E-5 0.9060231825024763 2.899552541051865E-5 80-81 5.79910508210373E-5 8.698657623155594E-5 2.899552541051865E-5 1.009885154522954 2.899552541051865E-5 82-83 5.79910508210373E-5 8.698657623155594E-5 2.899552541051865E-5 1.1501945019844537 2.899552541051865E-5 84-85 8.698657623155594E-5 8.698657623155594E-5 2.899552541051865E-5 1.3579329437881147 2.899552541051865E-5 86-87 8.698657623155594E-5 8.698657623155594E-5 2.899552541051865E-5 1.5844169927696758 2.899552541051865E-5 88-89 8.698657623155594E-5 8.698657623155594E-5 2.899552541051865E-5 1.8710232636899473 2.899552541051865E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11620 0.0 26.247149 1 TACATGG 12195 0.0 24.542469 2 ACATGGG 11925 0.0 23.942839 3 GTACGCT 160 1.858898E-6 23.753605 3 TAGATCG 190 3.2851676E-7 22.504068 5 GAGTACT 8030 0.0 21.684658 12-13 GTGTAGC 2495 0.0 21.51598 1 TAGCCCT 2530 0.0 20.843388 4 GTATCAA 20455 0.0 20.78628 1 CATGGGG 7065 0.0 20.57667 4 AGAGTAC 13985 0.0 20.418116 10-11 GTACTTT 8885 0.0 19.599943 14-15 AGTACTT 8850 0.0 19.353352 12-13 GTAGCCC 2185 0.0 19.350763 3 TCAACGC 22430 0.0 18.681217 4 ATCAACG 22495 0.0 18.648087 3 CAACGCA 22540 0.0 18.569239 5 AACGCAG 22810 0.0 18.287485 6 TATCAAC 23160 0.0 18.153664 2 GAGTACA 9000 0.0 16.785637 1 >>END_MODULE