FastQCFastQC Report
Thu 26 May 2016
SRR936727_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936727_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3448808
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT98870.2866787597337979No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78540.22773085657421346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65180.18899283462576055No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47740.13842463830981602No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40710.11804078394622142No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39120.11343049540594895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG114250.022.7053991
TACATGG116600.021.7152442
ACATGGG114300.021.0279163
GAGTACT100000.020.66242212-13
AGAGTAC159850.019.43320710-11
GTATCAA224050.019.1697391
GTACTTT107000.019.15585714-15
AGTACTT106750.018.88868712-13
TAAGGTG14350.017.2114815
CATGGGG68400.017.0827734
TCAACGC250650.016.9034944
CAACGCA251800.016.8069445
ATCAACG252150.016.8034273
TATCAAC258500.016.4658262
AACGCAG257700.016.439396
GTAAGGT12900.016.201024
GTATAGG9100.016.185011
ATTCGCG5150.016.140680-81
TACTTTT123600.016.12196714-15
ACTTTTT130700.015.80881516-17
Produced by FastQC (version 0.11.5)