##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936727_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3448808 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.867640355740303 31.0 31.0 34.0 30.0 34.0 2 32.003918455303975 31.0 31.0 34.0 30.0 34.0 3 32.126212302917416 33.0 31.0 34.0 30.0 34.0 4 35.85151623401477 37.0 35.0 37.0 35.0 37.0 5 35.701836692561606 37.0 35.0 37.0 33.0 37.0 6 35.73917423063273 37.0 35.0 37.0 33.0 37.0 7 35.66225867024201 37.0 35.0 37.0 33.0 37.0 8 35.625941774665336 37.0 35.0 37.0 33.0 37.0 9 37.23804775447053 39.0 37.0 39.0 33.0 39.0 10-11 37.13242676890103 39.0 37.0 39.0 33.0 39.0 12-13 37.00655240883227 39.0 37.0 39.0 33.0 39.0 14-15 38.11374451694614 40.0 37.0 41.0 33.0 41.0 16-17 38.08807028399377 40.0 37.0 41.0 33.0 41.0 18-19 38.201000026675885 40.0 37.5 41.0 33.0 41.0 20-21 38.197375296044314 40.0 37.5 41.0 33.0 41.0 22-23 38.09805286348211 40.0 37.0 41.0 33.0 41.0 24-25 37.988162721728784 40.0 37.0 41.0 33.0 41.0 26-27 37.78694479367944 40.0 37.0 41.0 32.5 41.0 28-29 37.82661951607628 40.0 37.0 41.0 33.0 41.0 30-31 37.734147711325186 40.0 37.0 41.0 33.0 41.0 32-33 37.57320224842903 40.0 37.0 41.0 32.0 41.0 34-35 37.406171059682066 39.5 36.0 41.0 32.0 41.0 36-37 37.24456652849332 39.0 36.0 41.0 31.0 41.0 38-39 37.058338272237826 39.0 36.0 41.0 31.0 41.0 40-41 36.853485320145396 39.0 35.5 41.0 30.5 41.0 42-43 36.62265339212853 39.0 35.0 40.0 30.0 41.0 44-45 36.37638511624886 39.0 35.0 40.0 30.0 41.0 46-47 36.067937530880236 38.5 35.0 40.0 29.5 41.0 48-49 35.72951712591713 38.0 35.0 40.0 28.5 41.0 50-51 35.437374739330224 38.0 34.5 40.0 27.5 41.0 52-53 35.182234702540704 38.0 34.0 40.0 27.0 41.0 54-55 34.8514547635009 38.0 34.0 40.0 26.0 41.0 56-57 34.50344814788182 38.0 33.0 40.0 26.0 41.0 58-59 34.134408468085205 37.0 33.0 40.0 25.0 41.0 60-61 33.738631289419416 36.5 32.5 39.0 24.5 40.5 62-63 33.40258025381523 36.0 32.0 39.0 23.5 40.0 64-65 33.07779919902761 36.0 32.0 39.0 23.0 40.0 66-67 32.62018442313982 35.0 31.0 38.5 22.0 40.0 68-69 32.15932055365216 35.0 31.0 38.0 21.5 40.0 70-71 31.642555050904544 35.0 30.5 37.5 20.0 39.5 72-73 31.06931162882944 34.0 30.0 37.0 18.0 39.0 74-75 30.51794083637013 34.0 29.0 36.0 17.5 39.0 76-77 29.12839784064523 32.5 28.0 34.5 14.0 36.5 78-79 29.928276378389285 34.0 29.0 35.0 14.5 37.0 80-81 29.775699604037104 34.0 29.0 35.0 12.0 37.0 82-83 29.364413443717368 34.0 29.0 35.0 7.5 36.0 84-85 28.91083455501147 33.0 29.0 35.0 6.5 36.0 86-87 28.474609053330887 33.0 28.0 35.0 2.0 35.5 88-89 27.965194496185347 33.0 27.0 35.0 2.0 35.0 90-91 27.37612531634118 32.0 26.0 34.0 2.0 35.0 92-93 26.764871660005426 32.0 25.0 34.0 2.0 35.0 94-95 25.980822069538227 32.0 24.5 34.0 2.0 35.0 96-97 25.107280544466377 31.0 21.5 34.0 2.0 35.0 98-99 23.908411398952914 31.0 9.5 34.0 2.0 35.0 100-101 20.08816234478695 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26247171629829325 1101 2 0.4161742933547927 1101 3 0.30516222912482505 1101 4 0.28607534077215035 1101 5 0.2610274916842741 1101 6 0.30652919355186015 1101 7 0.27026551986841696 1101 8 0.22460067297117803 1101 9 0.2762799587573923 1101 10-11 0.29131278496766555 1101 12-13 0.34577283116635726 1101 14-15 0.35581976197117626 1101 16-17 0.40334675832021816 1101 18-19 0.4024496512090394 1101 20-21 0.2611357668447667 1101 22-23 0.33909453009367496 1101 24-25 0.366608405770549 1101 26-27 0.24825284224481692 1101 28-29 0.3036913820712215 1101 30-31 0.3897264773903757 1101 32-33 0.3648902095067399 1101 34-35 0.4316540814809855 1101 36-37 0.4172053252622945 1101 38-39 0.3683128045806967 1101 40-41 0.37497888315930084 1101 42-43 0.47944573358006437 1101 44-45 0.4328412956751464 1101 46-47 0.27755376510726393 1101 48-49 0.38161866969227987 1101 50-51 0.41952891579505547 1101 52-53 0.5319479781842134 1101 54-55 0.4629418917888586 1101 56-57 0.5193221567988289 1101 58-59 0.43746248301228974 1101 60-61 0.38903460162096337 1101 62-63 0.42194601627719663 1101 64-65 0.5277484588435399 1101 66-67 0.5903503043561003 1101 68-69 0.47795864292663026 1101 70-71 0.4507366449539312 1101 72-73 0.5383869280302811 1101 74-75 0.5954588672723808 1101 76-77 0.570987061319638 1101 78-79 0.6196872055824407 1101 80-81 0.6170808157127361 1101 82-83 0.6253820736656586 1101 84-85 0.5589128053385402 1101 86-87 0.6231791320098417 1101 88-89 0.46485216488648007 1101 90-91 0.5101558014989962 1101 92-93 0.44091862148365735 1101 94-95 0.27055070106898427 1101 96-97 0.47131414594377574 1101 98-99 -0.12684024407591465 1101 100-101 0.30436836297405634 1102 1 0.22639757832003937 1102 2 0.3679997328620175 1102 3 0.28531954544802574 1102 4 0.21661458831287916 1102 5 0.10284317128033393 1102 6 0.1693301137720553 1102 7 0.25317185708828305 1102 8 0.2020384099664696 1102 9 0.23088361175270933 1102 10-11 0.291823437462547 1102 12-13 0.30899685722930315 1102 14-15 0.4602313289597788 1102 16-17 0.4179642482304118 1102 18-19 0.3633126875373165 1102 20-21 0.2926704879777091 1102 22-23 0.2736747418737906 1102 24-25 0.17866310982365263 1102 26-27 0.29637935011374594 1102 28-29 0.2936397939693478 1102 30-31 0.41205834300237854 1102 32-33 0.279834886744986 1102 34-35 0.39088759233515447 1102 36-37 0.4272156764946047 1102 38-39 0.35061107692703786 1102 40-41 0.3791778235118102 1102 42-43 0.4225350255632492 1102 44-45 0.46014779049767185 1102 46-47 0.4360719329827063 1102 48-49 0.5482730742256621 1102 50-51 0.4623574040296319 1102 52-53 0.5881433432223204 1102 54-55 0.5098471658755486 1102 56-57 0.5330954268421237 1102 58-59 0.576026145269573 1102 60-61 0.42212188311223287 1102 62-63 0.44453694279651046 1102 64-65 0.3724856695007617 1102 66-67 0.5504673734946195 1102 68-69 0.3816062229915147 1102 70-71 0.22597199077099006 1102 72-73 0.20048735054480105 1102 74-75 0.3085459869283902 1102 76-77 0.2580824109211477 1102 78-79 0.27080271736778627 1102 80-81 0.3968073078960934 1102 82-83 0.4728317989191808 1102 84-85 0.32540805111650783 1102 86-87 0.410217279070789 1102 88-89 0.22520258802344273 1102 90-91 0.2684973510840756 1102 92-93 0.33543589179585 1102 94-95 0.443988026006803 1102 96-97 0.3893188478805705 1102 98-99 0.21022133797580622 1102 100-101 0.08726063297502762 1103 1 0.20556129808498014 1103 2 0.276951891159122 1103 3 0.2555913533290095 1103 4 0.16357584694748795 1103 5 0.16481762014032597 1103 6 0.11069895351226933 1103 7 0.1406092691653953 1103 8 0.1549605073956002 1103 9 0.11751806363692197 1103 10-11 0.21292759678292583 1103 12-13 0.15044961009147073 1103 14-15 0.3192395950458007 1103 16-17 0.28801105158695606 1103 18-19 0.2596859900990509 1103 20-21 0.29568842032837495 1103 22-23 0.21212775769259906 1103 24-25 0.19621825706747842 1103 26-27 0.1640022067135618 1103 28-29 0.24271300138236995 1103 30-31 0.2856441732802395 1103 32-33 0.21576251834118665 1103 34-35 0.22212504951319545 1103 36-37 0.25422783319236686 1103 38-39 0.19911132429903944 1103 40-41 0.3469946983265686 1103 42-43 0.49192267448128746 1103 44-45 0.3462147293436857 1103 46-47 0.35176160070151496 1103 48-49 0.3678231420326128 1103 50-51 0.40257682247881377 1103 52-53 0.45312129262033807 1103 54-55 0.4296462403974388 1103 56-57 0.3584469796374634 1103 58-59 0.1445584988499533 1103 60-61 0.005470114882754729 1103 62-63 0.2655892976431957 1103 64-65 0.3550164055713978 1103 66-67 0.17140809833149007 1103 68-69 0.2398948198405364 1103 70-71 0.017989350179913544 1103 72-73 0.171960975858223 1103 74-75 0.17517923351836018 1103 76-77 0.1620567222946434 1103 78-79 0.06845748071685875 1103 80-81 0.2884872520133861 1103 82-83 0.23523899476979793 1103 84-85 0.2845354010056802 1103 86-87 0.16597147638904275 1103 88-89 0.19820405798224527 1103 90-91 0.26876134468137636 1103 92-93 0.274155219085511 1103 94-95 0.3473675893279662 1103 96-97 0.4086330476557727 1103 98-99 0.39504284066908824 1103 100-101 0.35112546630131547 1104 1 0.24810948411094813 1104 2 0.30039656087302546 1104 3 0.26483405953656813 1104 4 0.17795036908216133 1104 5 0.07629337925957458 1104 6 0.09330049400028884 1104 7 0.13931702143112545 1104 8 0.1390806747830169 1104 9 0.2183067286989555 1104 10-11 0.2147466754419085 1104 12-13 0.16850972929563568 1104 14-15 0.14712179576200413 1104 16-17 0.16292629811275816 1104 18-19 0.12668018524898628 1104 20-21 0.17728379420355367 1104 22-23 0.15389726597264541 1104 24-25 0.16601106464629822 1104 26-27 0.23671708104537714 1104 28-29 0.3001764117401109 1104 30-31 0.2721635159372866 1104 32-33 0.33094651244645235 1104 34-35 0.29144368151722233 1104 36-37 0.327503939204405 1104 38-39 0.32234981142039487 1104 40-41 0.3746742674959478 1104 42-43 0.37351139014572254 1104 44-45 0.4238630604076761 1104 46-47 0.35214666722258414 1104 48-49 0.3124029700954196 1104 50-51 0.3873908309103271 1104 52-53 0.4197508952472262 1104 54-55 0.24395040743863206 1104 56-57 0.3468264599197255 1104 58-59 0.4248192343824968 1104 60-61 0.2758500656785827 1104 62-63 0.14087352001411801 1104 64-65 0.2975524403627645 1104 66-67 0.2970110825415446 1104 68-69 0.4330714299900862 1104 70-71 0.3988795085676493 1104 72-73 0.4899768179609758 1104 74-75 0.5076661418114945 1104 76-77 0.33249054911236087 1104 78-79 0.46418657143831155 1104 80-81 0.30009084877882586 1104 82-83 0.34298410292688075 1104 84-85 0.38883987876337045 1104 86-87 0.4241546948127599 1104 88-89 0.40186013698413703 1104 90-91 0.5088727920321325 1104 92-93 0.441933252094703 1104 94-95 0.3816895559858686 1104 96-97 0.6304996104543541 1104 98-99 0.28491520276921634 1104 100-101 0.1129043959495526 1105 1 0.11219595627677137 1105 2 0.2171140786166177 1105 3 0.1335581740461933 1105 4 0.04977223709616396 1105 5 0.13728583785787407 1105 6 0.10995937918543319 1105 7 0.12593480350379593 1105 8 0.14242357511611203 1105 9 0.13610520883687371 1105 10-11 0.11477623300738315 1105 12-13 0.042007269985546714 1105 14-15 0.11406871640657812 1105 16-17 0.19712259171469526 1105 18-19 0.2148585464569166 1105 20-21 0.14828082517626484 1105 22-23 0.110810478928272 1105 24-25 0.08245176674102339 1105 26-27 0.16984833714540315 1105 28-29 0.09836205979274837 1105 30-31 0.17777860887471064 1105 32-33 0.1530325285812637 1105 34-35 -0.07095004697060148 1105 36-37 0.14793605874726268 1105 38-39 0.09130972291660555 1105 40-41 0.15945622314511354 1105 42-43 0.22640350744462268 1105 44-45 0.06645008330936264 1105 46-47 0.19253315953580596 1105 48-49 0.045646967195160926 1105 50-51 0.3168951324409264 1105 52-53 0.24034612429422708 1105 54-55 0.20466725681931308 1105 56-57 0.29439338970904316 1105 58-59 0.2802321897650515 1105 60-61 0.06152646945181317 1105 62-63 0.2515027824865044 1105 64-65 0.05935477625494201 1105 66-67 0.17798451571912466 1105 68-69 0.06386064111676149 1105 70-71 0.044614894764389845 1105 72-73 0.1751859105919742 1105 74-75 0.16875722616395805 1105 76-77 0.09224412346652855 1105 78-79 0.05782493884729334 1105 80-81 0.24314747504211098 1105 82-83 0.26532141868975145 1105 84-85 0.3536336212457982 1105 86-87 0.319218521057703 1105 88-89 0.29295950951924965 1105 90-91 0.21126556743926983 1105 92-93 0.08370116850349163 1105 94-95 0.23954420756249206 1105 96-97 0.3193725141292596 1105 98-99 0.17403717417438358 1105 100-101 -0.042964530121547995 1106 1 0.06436853382940555 1106 2 0.0889268436346029 1106 3 0.09991375829162052 1106 4 0.07188712561763566 1106 5 0.0885532447551185 1106 6 0.05274657049272946 1106 7 0.07293192625137124 1106 8 0.08923987970651837 1106 9 0.11716777647870913 1106 10-11 0.12312020505556376 1106 12-13 0.18081490202359873 1106 14-15 0.14118556069178823 1106 16-17 0.1469431533908434 1106 18-19 0.14646950785277824 1106 20-21 -0.01940977779889863 1106 22-23 0.044101237404646554 1106 24-25 0.13135059976199415 1106 26-27 0.11482340415729908 1106 28-29 0.2313457753599124 1106 30-31 0.025333664224028496 1106 32-33 0.03421352191726612 1106 34-35 0.12460664978043212 1106 36-37 0.10810453039588452 1106 38-39 0.22365337580333744 1106 40-41 -0.0160478359645424 1106 42-43 0.08863087037917694 1106 44-45 0.06359994760575205 1106 46-47 0.11250498693905087 1106 48-49 0.1353617496494337 1106 50-51 -0.035134542374350985 1106 52-53 -0.07180936732238052 1106 54-55 -0.1295379111963939 1106 56-57 -0.08529750363103972 1106 58-59 -0.09364642569820347 1106 60-61 -0.03876119579823012 1106 62-63 -0.08410645591749955 1106 64-65 -0.09718509372876838 1106 66-67 -0.30539005263301533 1106 68-69 -0.06405890620192167 1106 70-71 -0.1738997086715166 1106 72-73 -0.1216412526867039 1106 74-75 -0.13570785445429934 1106 76-77 -0.29663352011412414 1106 78-79 -0.3793338523707277 1106 80-81 -0.30244083788968723 1106 82-83 -0.2287889727749608 1106 84-85 -0.09995886478188964 1106 86-87 -0.05978617517498819 1106 88-89 -0.09107779877390954 1106 90-91 0.03866587748181516 1106 92-93 -0.11699750709649592 1106 94-95 0.01232135608294982 1106 96-97 0.22321259024806395 1106 98-99 0.2855765402189654 1106 100-101 0.2328694291361728 1107 1 0.05263389711264921 1107 2 0.13176404177063006 1107 3 0.03481935943413106 1107 4 0.06638872304205279 1107 5 -0.034081674511853066 1107 6 0.07694327222680641 1107 7 8.163699844843109E-4 1107 8 0.039682233922299304 1107 9 0.03579780805785049 1107 10-11 0.029553577991066504 1107 12-13 0.058467950057135454 1107 14-15 0.12048097032803895 1107 16-17 0.12186940716895123 1107 18-19 0.153247850594191 1107 20-21 0.1164943213337537 1107 22-23 0.1045013937614101 1107 24-25 0.16012415330803975 1107 26-27 0.10933038788758154 1107 28-29 0.016350269077932467 1107 30-31 0.16393925736402792 1107 32-33 0.09863417085939119 1107 34-35 0.1064307299532885 1107 36-37 0.16632431014214433 1107 38-39 0.08566175997313508 1107 40-41 0.14474204270507585 1107 42-43 0.18571290843419774 1107 44-45 0.15087897056065458 1107 46-47 0.15895488813881542 1107 48-49 0.08308143398112833 1107 50-51 0.0410787307144318 1107 52-53 0.04845434621232414 1107 54-55 0.17563999079130355 1107 56-57 0.19557772490222192 1107 58-59 0.23773508480663708 1107 60-61 0.06140645681890078 1107 62-63 -0.08111644058961076 1107 64-65 0.006600802152405549 1107 66-67 0.16784450098072767 1107 68-69 0.0828952763415245 1107 70-71 0.016055046359294778 1107 72-73 0.09525749707476194 1107 74-75 -0.06558323072624006 1107 76-77 -0.11535351839050989 1107 78-79 0.08624266702920735 1107 80-81 0.1507345894752099 1107 82-83 0.2835833409973638 1107 84-85 0.21513904286912577 1107 86-87 0.35059129713939896 1107 88-89 0.1317362228574943 1107 90-91 0.10716408307249381 1107 92-93 0.24195256338716575 1107 94-95 0.39320880383578327 1107 96-97 0.47592688827390717 1107 98-99 0.6644393217688673 1107 100-101 0.38565953556957666 1108 1 -0.004128429347726836 1108 2 0.08580932529761753 1108 3 -0.07111866654729937 1108 4 0.004053776503297968 1108 5 0.022673762815038856 1108 6 -0.04288262058140191 1108 7 0.02315094075232338 1108 8 0.0010716468898408493 1108 9 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-1.1853130058637404 2316 88-89 -1.199967422742386 2316 90-91 -1.4143716899099381 2316 92-93 -1.1388246878988255 2316 94-95 -1.3234160280781566 2316 96-97 -1.089561361710082 2316 98-99 -0.4537250717339383 2316 100-101 -0.31047074980320133 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 17.0 4 55.0 5 104.0 6 212.0 7 541.0 8 1313.0 9 2366.0 10 3748.0 11 5891.0 12 8471.0 13 11633.0 14 15141.0 15 19355.0 16 23453.0 17 29122.0 18 27025.0 19 22263.0 20 21934.0 21 23562.0 22 25704.0 23 28786.0 24 34134.0 25 41241.0 26 51027.0 27 62729.0 28 77250.0 29 94499.0 30 115599.0 31 142478.0 32 174906.0 33 221619.0 34 287861.0 35 385719.0 36 533532.0 37 638879.0 38 306584.0 39 10049.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.25156552044529 19.34363848004886 15.01661362717617 23.388182372329684 2 15.840862067215609 20.419144225457487 39.034959133872995 24.70503457345391 3 19.808068711343346 23.632050988363495 29.590098096925445 26.969782203367714 4 13.532569898266006 16.775322880609416 36.59654755076986 33.09555967035471 5 14.248477869850007 37.08978168375127 33.924893457413845 14.736846988984873 6 32.8989900278589 35.86154984562782 16.81215654800151 14.427303578511763 7 29.253835900555526 32.19604853747638 20.171514779492803 18.378600782475292 8 26.55805498763461 34.62401764818967 19.241119725209707 19.57680763896601 9 26.03097364505888 17.09039339156223 20.165779257324935 36.712853706053956 10-11 25.411852442931004 26.26601712823677 27.002242513935247 21.319887914896974 12-13 26.382448660522705 23.353039658919837 27.499370797098592 22.765140883458866 14-15 22.86978371853573 25.10711718563567 25.88970982021826 26.133389275610337 16-17 22.699371737753395 28.3136146877631 26.382287855834164 22.60472571864934 18-19 22.522853626460552 27.154847645148237 28.356590367819685 21.965708360571533 20-21 23.331443781732144 26.063967362466258 28.45044203909197 22.154146816709627 22-23 22.81500558455763 26.051018964299427 28.414959887450575 22.71901556369237 24-25 23.101907306067545 26.214360336709298 28.401634079884808 22.28209827733835 26-27 22.812853791177144 26.344223438650534 28.509829568428586 22.333093201743733 28-29 22.4716097314867 26.865653465475724 28.314590126145145 22.34814667689243 30-31 22.744285933202978 26.21506692996663 28.31806445823124 22.722582678599156 32-33 22.533904584804848 26.40240752599795 28.389570460977087 22.674117428220114 34-35 23.232458838996116 26.382868230855372 27.997282512568233 22.387390417580278 36-37 23.12757990380344 26.095563870270343 28.258087226409863 22.51876899951635 38-39 22.860061621723347 26.490309243852945 28.183811995604618 22.46581713881909 40-41 23.019873248923467 26.427558863108313 28.019834104952814 22.532733783015402 42-43 22.951808626313884 26.488611815875316 27.708735048930773 22.850844508880026 44-45 23.084634055354453 26.41056396461261 27.625089908904393 22.879712071128548 46-47 23.33348217703044 26.76343536665422 27.266290266086134 22.636792190229205 48-49 23.42582357996755 26.75440249785138 27.12977321467302 22.69000070750805 50-51 22.925034073707653 26.855416860515774 27.372107794263673 22.847441271512903 52-53 23.43289104256076 26.963422211925526 26.89819638035054 22.705490365163172 54-55 23.126001434701095 26.667506571847266 27.10641260356495 23.10007938988669 56-57 23.263125049660626 26.914870232797416 26.97020014464654 22.851804572895418 58-59 23.647879366310097 26.68370003202636 26.825448213200165 22.842972388463377 60-61 23.301362047805423 26.728250838910974 27.475235000184856 22.495152113098754 62-63 23.066816999127667 26.778991710306236 27.391842468127685 22.762348822438412 64-65 22.91326680329913 27.026898501102455 27.117368212886834 22.942466482711584 66-67 23.103724186604108 26.90886727182023 26.854036073477282 23.133372468098376 68-69 22.99697991894741 26.720113107696893 27.15705421836356 23.125852754992138 70-71 23.225098267553648 26.674216545427363 26.964680515005927 23.136004672013055 72-73 23.242040988766494 26.552983282691077 27.189295331778546 23.015680396763884 74-75 23.001792062727993 27.14186293329931 26.77341715624583 23.082927847726864 76-77 23.095255182112425 26.85005128651219 26.80345486243254 23.251238668942843 78-79 23.15271043094434 26.85439397129126 26.822438660011144 23.170456937753258 80-81 23.251150579813356 26.969585025760463 26.71257483988151 23.066689554544677 82-83 23.196075975206917 26.862873705600716 26.918126345432952 23.02292397375942 84-85 23.1219395503427 26.954312991348285 26.769955672184388 23.153791786124632 86-87 23.083208458401568 26.974913476750068 26.686869922848167 23.255008142000193 88-89 23.058695570543087 26.94020981996646 26.480343350725782 23.52075125876467 90-91 23.12909822844164 26.792817493314157 26.682074253646675 23.396010024597526 92-93 23.217462622667085 26.706240832634887 26.875129849107545 23.201166695590487 94-95 22.965053441137186 26.703475663742793 26.848134906269422 23.483335988850598 96-97 22.8129954616151 26.630927707112377 27.036677062541102 23.51939976873142 98-99 22.812014729251633 26.81858690172926 26.535545069732557 23.833853299286545 100-101 22.606536442732768 26.75277769240003 26.55360269270449 24.087083172162707 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 204.0 1 150.5 2 134.0 3 190.5 4 175.5 5 168.5 6 296.5 7 615.0 8 1040.5 9 1319.5 10 1347.0 11 1313.0 12 1455.0 13 1643.5 14 1853.5 15 2179.0 16 2470.5 17 2770.5 18 3077.0 19 3370.0 20 3861.0 21 4577.0 22 5393.5 23 6677.0 24 8451.0 25 10538.5 26 13383.0 27 17290.5 28 21357.0 29 26231.5 30 32361.0 31 39537.0 32 47330.0 33 53462.5 34 60204.0 35 67494.0 36 75326.0 37 82908.0 38 87759.5 39 91936.5 40 96792.0 41 104779.0 42 112477.5 43 118519.5 44 123393.0 45 127119.0 46 130410.0 47 139442.5 48 156671.5 49 155782.5 50 140073.0 51 131060.5 52 125500.5 53 122073.0 54 118975.5 55 112646.5 56 103300.0 57 93672.0 58 85385.5 59 75118.5 60 62399.0 61 50790.5 62 41823.5 63 33307.0 64 25099.5 65 19503.5 66 14960.5 67 11959.0 68 9039.0 69 6011.5 70 4398.5 71 3387.0 72 2755.5 73 2301.5 74 1829.5 75 1448.5 76 1038.5 77 647.5 78 423.0 79 280.5 80 168.5 81 111.0 82 71.5 83 47.5 84 31.0 85 17.5 86 8.5 87 4.0 88 1.5 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012584058028165094 2 0.010235420469913083 3 0.0051612035230723195 4 2.899552541051865E-4 5 0.005422163251766987 6 0.0 7 2.609597286946678E-4 8 0.0020006912533257868 9 1.4497762705259324E-4 10-11 0.0 12-13 0.0 14-15 4.3493288115777973E-4 16-17 0.004885746031672392 18-19 0.0019282024397994902 20-21 5.219194573893356E-4 22-23 3.479463049262238E-4 24-25 4.929239319788171E-4 26-27 1.304798643473339E-4 28-29 8.698657623155594E-5 30-31 4.7842616927355774E-4 32-33 0.0035519518627885344 34-35 9.858478639576341E-4 36-37 0.0010438389147786713 38-39 0.0054076654890617285 40-41 2.8995525410518647E-5 42-43 0.0016817404738100818 44-45 0.005175701285777579 46-47 0.0 48-49 0.0024356241344835666 50-51 0.0010003456266628934 52-53 6.089060336208916E-4 54-55 1.739731524631119E-4 56-57 0.011656201215028496 58-59 0.002508112948009863 60-61 0.00407387132017787 62-63 2.1746644057888987E-4 64-65 0.00405937355747261 66-67 0.0012033143045365239 68-69 0.0017252337619258597 70-71 0.005436661014472247 72-73 0.0031025212189254954 74-75 0.007770800810018998 76-77 0.0013192964061785985 78-79 0.0070169171493455135 80-81 0.0032474988459780885 82-83 0.0030155346426939396 84-85 0.002044184541441565 86-87 6.958926098524476E-4 88-89 0.002595099524241419 90-91 0.008423200131755668 92-93 0.0027110816258834936 94-95 3.914395930420018E-4 96-97 1.1598210164207459E-4 98-99 7.103903725577069E-4 100-101 0.0023776330836625293 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3448808.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.820267435985514 #Duplication Level Percentage of deduplicated Percentage of total 1 71.17379200611569 35.458973521778915 2 15.242440909921456 15.187649650189867 3 5.695743135442792 8.512903387633157 4 2.6880415240843165 5.35675790435659 5 1.4288760688684166 3.5593493941952077 6 0.8984814670448241 2.6857552184669844 7 0.5849594087781184 2.039998392916528 8 0.39879793671134167 1.5894575887902613 9 0.2857425104464362 1.2812191459444202 >10 1.4424755059368677 12.755320273832726 >50 0.1017884114850628 3.5103254644427637 >100 0.05430497430141246 4.827883438621228 >500 0.0029206031190614516 1.0625955820212303 >1k 0.00146030155732961 1.4658487704028507 >5k 1.752361868795532E-4 0.7059622664073129 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9887 0.2866787597337979 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7854 0.22773085657421346 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6518 0.18899283462576055 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4774 0.13842463830981602 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4071 0.11804078394622142 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3912 0.11343049540594895 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 8.698657623155594E-5 0.0 0.0 0.0 5 0.0 1.4497762705259324E-4 0.0 2.899552541051865E-5 0.0 6 0.0 1.4497762705259324E-4 0.0 2.899552541051865E-5 0.0 7 0.0 1.4497762705259324E-4 0.0 2.899552541051865E-5 0.0 8 0.0 1.4497762705259324E-4 0.0 2.899552541051865E-5 0.0 9 0.0 1.4497762705259324E-4 0.0 2.899552541051865E-5 0.0 10-11 0.0 1.4497762705259324E-4 0.0 2.899552541051865E-5 0.0 12-13 0.0 1.4497762705259324E-4 0.0 2.899552541051865E-5 0.0 14-15 0.0 1.4497762705259324E-4 0.0 2.899552541051865E-5 0.0 16-17 0.0 1.4497762705259324E-4 0.0 2.899552541051865E-5 0.0 18-19 0.0 1.4497762705259324E-4 0.0 2.899552541051865E-5 0.0 20-21 0.0 1.4497762705259324E-4 0.0 2.899552541051865E-5 0.0 22-23 0.0 1.4497762705259324E-4 0.0 7.248881352629662E-5 0.0 24-25 0.0 1.4497762705259324E-4 0.0 1.8847091516837122E-4 0.0 26-27 0.0 1.4497762705259324E-4 0.0 3.4794630492622383E-4 0.0 28-29 0.0 1.4497762705259324E-4 0.0 6.23403796326151E-4 0.0 30-31 0.0 1.4497762705259324E-4 0.0 0.0015077673213469698 2.899552541051865E-5 32-33 0.0 1.4497762705259324E-4 0.0 0.0025226107107151224 2.899552541051865E-5 34-35 0.0 1.4497762705259324E-4 0.0 0.0038129115914832023 2.899552541051865E-5 36-37 0.0 1.4497762705259324E-4 0.0 0.005233692336598616 2.899552541051865E-5 38-39 0.0 1.4497762705259324E-4 0.0 0.007857787386250554 2.899552541051865E-5 40-41 0.0 1.4497762705259324E-4 0.0 0.013178466299080725 2.899552541051865E-5 42-43 0.0 1.4497762705259324E-4 0.0 0.01819469219510045 2.899552541051865E-5 44-45 0.0 1.4497762705259324E-4 0.0 0.024472223446477738 2.899552541051865E-5 46-47 0.0 1.4497762705259324E-4 0.0 0.03712877028816913 2.899552541051865E-5 48-49 0.0 1.4497762705259324E-4 0.0 0.045696948046977395 2.899552541051865E-5 50-51 0.0 1.4497762705259324E-4 0.0 0.057092189533311216 2.899552541051865E-5 52-53 0.0 1.4497762705259324E-4 0.0 0.0714594723742232 2.899552541051865E-5 54-55 0.0 1.4497762705259324E-4 0.0 0.09136490056854427 2.899552541051865E-5 56-57 0.0 1.4497762705259324E-4 0.0 0.13530761932818527 2.899552541051865E-5 58-59 0.0 1.4497762705259324E-4 0.0 0.18025068371448916 2.899552541051865E-5 60-61 0.0 1.4497762705259324E-4 0.0 0.21684303678256373 2.899552541051865E-5 62-63 0.0 1.4497762705259324E-4 0.0 0.2590895173056894 2.899552541051865E-5 64-65 0.0 1.4497762705259324E-4 0.0 0.30494594074242465 2.899552541051865E-5 66-67 0.0 1.4497762705259324E-4 0.0 0.38000085826755214 2.899552541051865E-5 68-69 0.0 1.4497762705259324E-4 0.0 0.4727285485303908 2.899552541051865E-5 70-71 0.0 1.4497762705259324E-4 0.0 0.5250364763709665 2.899552541051865E-5 72-73 0.0 1.4497762705259324E-4 0.0 0.5798090238714362 2.899552541051865E-5 74-75 0.0 1.4497762705259324E-4 0.0 0.6761901503360002 2.899552541051865E-5 76-77 0.0 1.4497762705259324E-4 0.0 0.7900700763858122 2.899552541051865E-5 78-79 0.0 1.4497762705259324E-4 0.0 0.9080528692812124 2.899552541051865E-5 80-81 0.0 1.4497762705259324E-4 0.0 1.0114799084205326 2.899552541051865E-5 82-83 0.0 1.4497762705259324E-4 0.0 1.1515862872041587 2.899552541051865E-5 84-85 0.0 1.4497762705259324E-4 0.0 1.3584258677200935 2.899552541051865E-5 86-87 0.0 1.4497762705259324E-4 0.0 1.5825467813806973 2.899552541051865E-5 88-89 0.0 1.4497762705259324E-4 0.0 1.8669493923697695 2.899552541051865E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11425 0.0 22.705399 1 TACATGG 11660 0.0 21.715244 2 ACATGGG 11430 0.0 21.027916 3 GAGTACT 10000 0.0 20.662422 12-13 AGAGTAC 15985 0.0 19.433207 10-11 GTATCAA 22405 0.0 19.169739 1 GTACTTT 10700 0.0 19.155857 14-15 AGTACTT 10675 0.0 18.888687 12-13 TAAGGTG 1435 0.0 17.211481 5 CATGGGG 6840 0.0 17.082773 4 TCAACGC 25065 0.0 16.903494 4 CAACGCA 25180 0.0 16.806944 5 ATCAACG 25215 0.0 16.803427 3 TATCAAC 25850 0.0 16.465826 2 AACGCAG 25770 0.0 16.43939 6 GTAAGGT 1290 0.0 16.20102 4 GTATAGG 910 0.0 16.18501 1 ATTCGCG 515 0.0 16.1406 80-81 TACTTTT 12360 0.0 16.121967 14-15 ACTTTTT 13070 0.0 15.808815 16-17 >>END_MODULE