FastQCFastQC Report
Thu 26 May 2016
SRR936726_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936726_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3667968
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT90540.24683966708542712No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73220.19962006211613625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56030.15275487681462868No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54640.1489653126744835No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44540.12142963079285315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG125650.023.9417271
TACATGG128000.023.055062
ACATGGG126000.022.327283
TAGATCG1954.206031E-721.934225
GAGTACT85750.021.775112-13
CATGGGG76950.020.811624
GTATCAA212950.020.4870451
GTACTTT95600.019.57829714-15
GTATAGC13050.019.3009911
AGAGTAC151500.019.28868910-11
ATCAACG237100.018.1785983
CAACGCA237100.018.1597945
TCAACGC237600.018.1203424
TATCAAC242250.017.9098362
AACGCAG241000.017.8844226
AGTACTT95650.017.45991312-13
GGTATCA169000.017.2661671
TAGCGCG2504.546633E-617.1075234
GTAAGGT13700.016.9965254
TAGCCCT24800.016.6706374
Produced by FastQC (version 0.11.5)