##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936726_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3667968 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.2121392553043 31.0 31.0 34.0 28.0 34.0 2 31.268924919737575 31.0 31.0 34.0 28.0 34.0 3 31.314379242130794 31.0 31.0 34.0 28.0 34.0 4 34.9586694867567 37.0 35.0 37.0 32.0 37.0 5 34.87872631386097 37.0 35.0 37.0 32.0 37.0 6 34.982235395728644 37.0 35.0 37.0 32.0 37.0 7 34.8227001980388 37.0 35.0 37.0 32.0 37.0 8 34.7955993618265 37.0 35.0 37.0 32.0 37.0 9 36.29507400282663 39.0 35.0 39.0 32.0 39.0 10-11 36.15114213101096 39.0 35.0 39.0 31.0 39.0 12-13 36.05327213868823 39.0 35.0 39.0 30.0 39.0 14-15 37.111266919449676 39.0 36.0 41.0 31.0 41.0 16-17 37.04416791531442 39.0 36.0 41.0 31.0 41.0 18-19 36.97645944566583 39.0 36.0 41.0 31.0 41.0 20-21 36.86255373547425 39.0 36.0 41.0 30.0 41.0 22-23 36.747980080524144 39.0 36.0 41.0 30.0 41.0 24-25 36.603864455742254 39.0 36.0 41.0 30.0 41.0 26-27 36.41071909569549 39.0 35.5 40.0 29.5 41.0 28-29 36.52063363148206 39.0 36.0 41.0 30.0 41.0 30-31 36.4722687057248 39.0 36.0 41.0 30.0 41.0 32-33 36.342217271252096 39.0 35.0 40.0 30.0 41.0 34-35 36.189391374188645 39.0 35.0 40.0 29.5 41.0 36-37 35.96174312316792 38.5 35.0 40.0 28.5 41.0 38-39 35.78683987973723 38.0 35.0 40.0 27.5 41.0 40-41 35.64964075477212 38.0 35.0 40.0 27.0 41.0 42-43 35.41011481016192 38.0 34.0 40.0 27.0 41.0 44-45 35.17472139887807 38.0 34.0 40.0 26.0 41.0 46-47 34.899949781459384 38.0 34.0 40.0 25.0 41.0 48-49 34.70534693868649 38.0 33.5 40.0 24.0 41.0 50-51 34.022160634989184 37.5 33.0 39.5 23.5 40.5 52-53 34.21988073505548 37.5 33.0 39.5 24.0 40.5 54-55 34.59071180555556 38.0 34.0 40.0 24.0 41.0 56-57 34.517242925783435 38.0 34.0 40.0 24.0 41.0 58-59 34.28279445185999 38.0 33.0 40.0 24.0 41.0 60-61 33.95923737611669 37.0 33.0 40.0 23.0 41.0 62-63 33.634096453404176 37.0 33.0 40.0 22.5 41.0 64-65 33.327671888086265 36.0 32.0 39.0 22.0 41.0 66-67 32.96230596886342 36.0 32.0 39.0 21.5 41.0 68-69 32.576477357490575 35.5 31.5 39.0 20.5 40.0 70-71 32.17436493448143 35.0 31.0 38.0 20.0 40.0 72-73 31.75712070007154 35.0 31.0 37.5 18.5 39.5 74-75 31.32568400269577 35.0 31.0 37.0 17.5 39.0 76-77 30.904670924064767 35.0 30.0 36.5 15.0 39.0 78-79 30.456286832382396 34.0 30.0 36.0 11.0 38.5 80-81 30.03900674705995 34.0 29.5 35.5 7.5 37.0 82-83 29.633557599193885 34.0 29.0 35.0 7.0 37.0 84-85 29.25350193894821 34.0 29.0 35.0 3.5 36.0 86-87 28.91932794942595 34.0 29.0 35.0 2.0 36.0 88-89 28.55082691561104 34.0 29.0 35.0 2.0 36.0 90-91 28.202453238414293 34.0 27.5 35.0 2.0 35.0 92-93 27.825715764150615 34.0 27.0 35.0 2.0 35.0 94-95 27.393496617200583 33.0 26.5 35.0 2.0 35.0 96-97 26.81051238724002 33.0 25.0 35.0 2.0 35.0 98-99 26.14225369468872 33.0 24.5 35.0 2.0 35.0 100-101 24.074635193109646 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3952420245757544 1101 2 0.4063385085128459 1101 3 0.35388942197905493 1101 4 0.366674295303298 1101 5 0.5000987965583192 1101 6 0.37616480390043705 1101 7 0.3714472378672795 1101 8 0.34740842670689887 1101 9 0.6472049227113246 1101 10-11 0.5054618245807205 1101 12-13 0.5385029144543907 1101 14-15 0.6851510227378625 1101 16-17 0.7155187306411719 1101 18-19 0.8147470834619028 1101 20-21 0.8128966079004343 1101 22-23 0.6540593861308608 1101 24-25 0.5373354400578663 1101 26-27 0.6819384689667416 1101 28-29 0.7096899210337995 1101 30-31 0.49791102260687126 1101 32-33 0.54180006415897 1101 34-35 0.41200058798561656 1101 36-37 0.5268661754908734 1101 38-39 0.5456534307503489 1101 40-41 0.5037645256446908 1101 42-43 0.2989268504123217 1101 44-45 0.3981344468589185 1101 46-47 0.5194772953367846 1101 48-49 0.36260698484932874 1101 50-51 0.5866833919555319 1101 52-53 0.6889207050840511 1101 54-55 0.6770648874991423 1101 56-57 0.575578498550577 1101 58-59 0.5179228328488179 1101 60-61 0.4828149975143958 1101 62-63 0.5438892371729835 1101 64-65 0.5574866872200488 1101 66-67 0.5157927723290783 1101 68-69 0.42017556886257523 1101 70-71 0.3828474045400796 1101 72-73 0.4094951431871081 1101 74-75 0.38046576312279257 1101 76-77 0.3622387138104095 1101 78-79 0.43547510486049745 1101 80-81 0.38977451520166184 1101 82-83 0.4139742358145213 1101 84-85 0.48309348199721924 1101 86-87 0.45084782516410726 1101 88-89 0.46352764643696176 1101 90-91 0.34628068285210034 1101 92-93 0.16207011721623488 1101 94-95 0.15053177704348997 1101 96-97 0.07408292111433568 1101 98-99 0.20558591985529517 1101 100-101 0.47803734413887256 1102 1 0.19416059856880352 1102 2 0.29165533841617375 1102 3 0.28113995627773747 1102 4 0.31055786597039514 1102 5 0.27076343610928433 1102 6 0.329921129926376 1102 7 0.08752521854232498 1102 8 0.1338685431103741 1102 9 0.3561686846212737 1102 10-11 0.4524454660528363 1102 12-13 0.5402878892900489 1102 14-15 0.383568248966462 1102 16-17 0.2093033029730833 1102 18-19 0.28339526193737186 1102 20-21 0.06343559430725776 1102 22-23 -0.04332904471655041 1102 24-25 0.15220087819908912 1102 26-27 0.1779365498531078 1102 28-29 0.19406459984460867 1102 30-31 0.11632661133469924 1102 32-33 0.10428609197399652 1102 34-35 -0.01541818030051445 1102 36-37 -0.1213917001337208 1102 38-39 -0.20823684190692404 1102 40-41 -0.3563779136293377 1102 42-43 -0.019550744390208763 1102 44-45 -0.1264488828591439 1102 46-47 -0.094967295789111 1102 48-49 0.10431057147099665 1102 50-51 -0.08158486537736565 1102 52-53 -0.25240595937405175 1102 54-55 -0.10766825374037126 1102 56-57 -8.959320088237632E-4 1102 58-59 -0.2015393063805604 1102 60-61 -0.5664541221851067 1102 62-63 -0.36947570404033314 1102 64-65 -0.20830245683697513 1102 66-67 -0.2865286819998758 1102 68-69 -0.35185616837142675 1102 70-71 -0.20709156827525277 1102 72-73 -0.41422423017651866 1102 74-75 -0.4187123535777886 1102 76-77 -0.29897981736421286 1102 78-79 -0.3572654267950064 1102 80-81 -0.27616700028200825 1102 82-83 -0.38927419419085396 1102 84-85 -0.4449782193398768 1102 86-87 -0.5692864755214515 1102 88-89 -0.6423763765681763 1102 90-91 -0.9057324618767026 1102 92-93 -0.7022637300586325 1102 94-95 -0.5549012839689667 1102 96-97 -0.6941889408166269 1102 98-99 -0.7592398706187993 1102 100-101 -0.4242770291056708 1103 1 0.2865541654195063 1103 2 0.43441726807287395 1103 3 0.36842673944186544 1103 4 0.37362079411388294 1103 5 0.41876927769227024 1103 6 0.2594417304005887 1103 7 0.34369563207570764 1103 8 0.14515118487591394 1103 9 0.5098885267688971 1103 10-11 0.5208150970235437 1103 12-13 0.5914501840199549 1103 14-15 0.608613781658228 1103 16-17 0.7302520566237121 1103 18-19 0.5629827088559196 1103 20-21 0.49008890559413487 1103 22-23 0.5246149113891505 1103 24-25 0.5752333809072141 1103 26-27 0.5350803623942895 1103 28-29 0.5755960038977364 1103 30-31 0.4237928467209571 1103 32-33 0.5599703490290366 1103 34-35 0.45568985989000055 1103 36-37 0.6096151955475833 1103 38-39 0.49106613622235074 1103 40-41 0.4264157275758578 1103 42-43 0.4237756517441795 1103 44-45 0.5387792138769143 1103 46-47 0.5765243719412112 1103 48-49 0.6595659926807613 1103 50-51 0.14156350754948477 1103 52-53 0.44936872812474604 1103 54-55 0.6793127431665056 1103 56-57 0.7425087798409535 1103 58-59 0.7062905103288841 1103 60-61 0.8302598187610357 1103 62-63 0.724804505027933 1103 64-65 0.6312541588060512 1103 66-67 0.6272258006045277 1103 68-69 0.5989977335786278 1103 70-71 0.6726351904172887 1103 72-73 0.6869218509538939 1103 74-75 0.6612060874153087 1103 76-77 0.5653176421965327 1103 78-79 0.6622125850432958 1103 80-81 0.7686861378883272 1103 82-83 0.7115180629279685 1103 84-85 0.5382317738391507 1103 86-87 0.5286268602313022 1103 88-89 0.4626359892666443 1103 90-91 0.6229061050663134 1103 92-93 0.46416348139716845 1103 94-95 0.5704765689228992 1103 96-97 0.5392483085520787 1103 98-99 0.42075957835317723 1103 100-101 0.4842459168387663 1104 1 0.24463942003268357 1104 2 0.26861081193073844 1104 3 0.2795776174371696 1104 4 0.29956117543469674 1104 5 0.20470439266819085 1104 6 0.3725638438563337 1104 7 0.34773130593495694 1104 8 0.41306294206331273 1104 9 0.5209145881557973 1104 10-11 0.534527373178804 1104 12-13 0.41452587318570266 1104 14-15 0.4547262195702899 1104 16-17 0.2300575216767342 1104 18-19 0.33504357915562366 1104 20-21 0.39289258236313174 1104 22-23 0.35705195761820363 1104 24-25 0.4600572425086469 1104 26-27 0.4447519891867273 1104 28-29 0.36030217505996376 1104 30-31 0.4128919529578141 1104 32-33 0.27983618092543594 1104 34-35 0.248206373770266 1104 36-37 0.44920699364443806 1104 38-39 0.29647650327744657 1104 40-41 0.10925843613971864 1104 42-43 0.23557728437796044 1104 44-45 0.12758323319234677 1104 46-47 0.19135888323756234 1104 48-49 0.0034785114043742738 1104 50-51 -0.024132378750948646 1104 52-53 0.12090300293701972 1104 54-55 0.3207701266689611 1104 56-57 0.3418260995187907 1104 58-59 0.2957498419109257 1104 60-61 0.21251895591399972 1104 62-63 0.20009285579725855 1104 64-65 0.14858788282506907 1104 66-67 0.1474221942614662 1104 68-69 0.25790665411609837 1104 70-71 0.28425256226825724 1104 72-73 0.0029997979842306677 1104 74-75 -0.17225617442075958 1104 76-77 0.08844025041680226 1104 78-79 0.15432838980564867 1104 80-81 0.31337212176658014 1104 82-83 0.1713450502377043 1104 84-85 0.2499342139865739 1104 86-87 0.10757475796124183 1104 88-89 0.049626039150940215 1104 90-91 0.03917331066972096 1104 92-93 -0.14011942428800594 1104 94-95 -0.05005486611931076 1104 96-97 0.15865939167853327 1104 98-99 0.2600040662418124 1104 100-101 0.48342777244196 1105 1 0.09263495310964487 1105 2 0.17128329051482893 1105 3 0.2109054870630871 1105 4 0.28932104343386555 1105 5 0.35502313108472805 1105 6 0.30170842344259086 1105 7 0.3994139111837569 1105 8 0.07812578267284209 1105 9 0.42371299383241734 1105 10-11 0.4032417339841814 1105 12-13 0.16070561400847794 1105 14-15 0.38050224943129507 1105 16-17 0.2838352846135024 1105 18-19 0.14609413007834604 1105 20-21 -0.035171649092859525 1105 22-23 -0.2648025231601352 1105 24-25 -0.09787495156659531 1105 26-27 -0.05478458914490858 1105 28-29 -0.04073026096807553 1105 30-31 -0.021179980983291102 1105 32-33 -0.18343646817523762 1105 34-35 -0.1966908045989797 1105 36-37 -0.22589554224146013 1105 38-39 -0.27275656750288135 1105 40-41 -0.475663688419786 1105 42-43 -0.5012969759249657 1105 44-45 -0.31156548969428854 1105 46-47 -0.26617178947892484 1105 48-49 -0.09803409226951487 1105 50-51 -0.16125560256426752 1105 52-53 -0.05945109142124494 1105 54-55 -0.08448045163211049 1105 56-57 -0.057376821856856 1105 58-59 -0.12732265953274435 1105 60-61 -0.01691173401363244 1105 62-63 -0.24056209134327133 1105 64-65 -0.14673482279327743 1105 66-67 -0.47806390425139256 1105 68-69 -0.28713711800835995 1105 70-71 -0.11016820300962138 1105 72-73 -0.19545224572276965 1105 74-75 -0.13923968561365285 1105 76-77 -0.04390862544300944 1105 78-79 -0.14195496675275976 1105 80-81 0.06002192948687579 1105 82-83 0.0631471799007457 1105 84-85 -0.12727607229654225 1105 86-87 -0.004918920764520607 1105 88-89 -0.07119843110800161 1105 90-91 -0.013970497405427551 1105 92-93 -0.14120782975862411 1105 94-95 -0.08027404571260632 1105 96-97 0.016269408431156762 1105 98-99 -0.15806540829998283 1105 100-101 0.015235161803882846 1106 1 0.059317641289755585 1106 2 0.0693839299557375 1106 3 -0.009247684013551805 1106 4 0.2011165278007212 1106 5 0.2682274270135707 1106 6 0.29727191764678906 1106 7 0.38210662058392586 1106 8 0.2973646458619612 1106 9 0.46703716042641474 1106 10-11 0.468634436827017 1106 12-13 0.5251795810771753 1106 14-15 0.293930052232831 1106 16-17 0.18686693352467643 1106 18-19 0.26359241131472544 1106 20-21 0.3753375511091406 1106 22-23 0.29426979403067577 1106 24-25 0.12321922991997525 1106 26-27 0.09678492766111901 1106 28-29 -0.008308920176965273 1106 30-31 0.08749083505254873 1106 32-33 0.049084533865645597 1106 34-35 0.047379714396655004 1106 36-37 0.09251446113184869 1106 38-39 0.1702056616101757 1106 40-41 -0.05219016008367561 1106 42-43 0.14550842217583693 1106 44-45 0.07352688075284419 1106 46-47 0.17115603840269955 1106 48-49 -0.02886332051253504 1106 50-51 0.1574598742853226 1106 52-53 0.07693854840567127 1106 54-55 0.2699908857451305 1106 56-57 0.13460679361060102 1106 58-59 0.16986644367487003 1106 60-61 0.195228229792626 1106 62-63 0.23932567018089657 1106 64-65 0.13319287421218462 1106 66-67 0.2090678797948229 1106 68-69 0.2072080313601461 1106 70-71 0.12165456187095458 1106 72-73 0.0798496617837472 1106 74-75 -0.010832409953710709 1106 76-77 -0.03274722458136381 1106 78-79 0.007430182318298506 1106 80-81 0.07900252182318113 1106 82-83 0.1394430465530796 1106 84-85 0.1269258569099989 1106 86-87 0.31042333459136273 1106 88-89 0.30743885805192406 1106 90-91 0.4050808059142703 1106 92-93 0.27511584469774064 1106 94-95 0.1405547160812901 1106 96-97 0.22086464213230883 1106 98-99 0.41977362754291647 1106 100-101 0.5801889630600492 1107 1 0.445630473919703 1107 2 0.4203706467625494 1107 3 0.3968146603418141 1107 4 0.39874898413675197 1107 5 0.3376959627058014 1107 6 0.39091478747210573 1107 7 0.4918671042603009 1107 8 0.527135206129536 1107 9 0.7223971785157204 1107 10-11 0.7228697540651225 1107 12-13 0.7317460964432101 1107 14-15 0.7787227840361552 1107 16-17 0.8419534874974204 1107 18-19 0.9084231124421152 1107 20-21 0.8935456023662027 1107 22-23 0.8747362688779035 1107 24-25 0.9210712770310678 1107 26-27 0.9246264049743687 1107 28-29 0.8013028806646716 1107 30-31 0.780731855277061 1107 32-33 0.8437724706380791 1107 34-35 0.7585806649955629 1107 36-37 0.7939927911493143 1107 38-39 0.8742520807022771 1107 40-41 0.7517270009278079 1107 42-43 0.8008328535085738 1107 44-45 0.748444704937576 1107 46-47 0.8891142994622925 1107 48-49 0.8565368901246373 1107 50-51 0.7485321890027592 1107 52-53 0.8278109550871804 1107 54-55 0.8339246943779202 1107 56-57 0.7686764844686778 1107 58-59 0.9069042141987111 1107 60-61 0.9679955971162357 1107 62-63 0.9976107830796934 1107 64-65 0.7741879451808273 1107 66-67 0.7477614588512935 1107 68-69 0.8695781093475006 1107 70-71 0.8988361286898296 1107 72-73 0.8538030013997684 1107 74-75 0.717089194470276 1107 76-77 0.8485969304645984 1107 78-79 0.8241449176636095 1107 80-81 0.8797273079611934 1107 82-83 1.0243418936635749 1107 84-85 1.0590860413237557 1107 86-87 0.9020671999353276 1107 88-89 0.9939800453596668 1107 90-91 1.0340858335869605 1107 92-93 1.0061833487534173 1107 94-95 1.0706770085664878 1107 96-97 1.0659275677127376 1107 98-99 1.3506981396097828 1107 100-101 1.5041446548923965 1108 1 0.04330519455083959 1108 2 -0.04256334820412633 1108 3 -0.13933048547274396 1108 4 -0.11891423544604152 1108 5 0.11369585932558834 1108 6 0.03834091100499393 1108 7 0.12072840355570236 1108 8 -0.19629152476208844 1108 9 0.16005690901643277 1108 10-11 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-1.6270931618933915 2316 58-59 -1.745782948559544 2316 60-61 -1.9282835450159794 2316 62-63 -1.72056710275222 2316 64-65 -1.6510025173319072 2316 66-67 -2.552583733081569 2316 68-69 -2.1432661422408295 2316 70-71 -1.658032489790159 2316 72-73 -1.5462819715524958 2316 74-75 -1.816462298796857 2316 76-77 -2.412173433978751 2316 78-79 -2.274751972702475 2316 80-81 -2.3093811086353746 2316 82-83 -2.054092244604689 2316 84-85 -2.2630665954023392 2316 86-87 -2.2498266539727467 2316 88-89 -2.527756446163554 2316 90-91 -2.606152877149384 2316 92-93 -2.8560382237762134 2316 94-95 -2.895702254613724 2316 96-97 -3.1987982278187452 2316 98-99 -3.660738159741207 2316 100-101 -4.404170897380503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8438.0 3 3540.0 4 3570.0 5 4084.0 6 4628.0 7 5308.0 8 6542.0 9 8329.0 10 10407.0 11 12669.0 12 15151.0 13 17299.0 14 19206.0 15 21785.0 16 24749.0 17 28706.0 18 26399.0 19 22630.0 20 23976.0 21 26287.0 22 29088.0 23 33396.0 24 38851.0 25 45571.0 26 53237.0 27 63450.0 28 75199.0 29 90037.0 30 108897.0 31 133663.0 32 165191.0 33 207317.0 34 269716.0 35 360826.0 36 496067.0 37 639581.0 38 493418.0 39 70744.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78084044428508 18.24292801703629 14.361909384274968 23.614322154403656 2 15.50776405178452 19.62395340778251 40.0793731938795 24.788909346553474 3 19.72388314043052 23.339713440486413 29.213338241108065 27.723065177975 4 12.979946202116516 16.058256404214486 37.03722188449682 33.92457550917218 5 14.357510586213715 36.74772421975684 33.97691148493382 14.917853709095622 6 32.85123690768305 35.273903750755295 17.32090856144438 14.553950780117273 7 29.361115641019627 31.874923983893037 20.35486364005753 18.409096735029802 8 26.487398477852945 34.198786656438664 19.61202130209593 19.701793563612455 9 26.105780255562948 17.12385747742358 20.290268424808307 36.480093842205164 10-11 25.39863617754403 25.97032768342474 27.184394785715238 21.446641353315986 12-13 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26.06235604229739 27.952264389226727 22.815400965825315 38-39 22.81832716422338 26.4018184514916 27.928100604841788 22.851753779443232 40-41 23.078500198277922 26.323169425858307 27.83561622173756 22.76271415412622 42-43 22.907434640389233 26.40264093885993 27.508793769142226 23.181130651608616 44-45 23.09657582125654 26.325030344206358 27.463551509903716 23.11484232463339 46-47 23.278764013912152 26.612262747374295 27.170801715326565 22.938171523386988 48-49 23.28744883199473 26.634203408096884 27.109797143711262 22.968550616197124 50-51 22.84142892624419 26.732414533543846 27.3433497796151 23.082806760596863 52-53 23.29252466650127 26.86216748956496 26.844773482591002 23.000534361342773 54-55 23.01204809422765 26.473499836281583 27.112315196531622 23.402136872959144 56-57 23.109808330802352 26.78915243183941 26.932409494089505 23.16862974326873 58-59 23.49894134973955 26.54748876598404 26.851206610470683 23.102363273805725 60-61 23.21318593357196 26.515558192216393 27.470903959962257 22.800351914249394 62-63 22.95421463778235 26.650138675363333 27.369661107565353 23.025985579288964 64-65 22.779374920167495 26.915111105718893 27.13753111337736 23.16798286073626 66-67 22.953353191353624 26.772925387380663 26.842202623510662 23.431518797755047 68-69 22.823699352227084 26.61197874810799 27.187213420433753 23.377108479231172 70-71 23.07139019466087 26.52617179677027 27.02504251096124 23.37739549760762 72-73 23.050203145303282 26.41250192893977 27.285814072563035 23.251480853193915 74-75 22.800816664937678 27.008764957183978 26.917171652178823 23.273246725699522 76-77 22.96099936120842 26.66967114093411 26.894420538607903 23.474908959249568 78-79 23.057349785583302 26.699174623884414 26.92196399370332 23.321511596828966 80-81 23.121299643222308 26.801535097137968 26.82217794599291 23.25498731364682 82-83 23.069914109954418 26.764265727764297 27.011550974704658 23.154269187576627 84-85 23.018287736912665 26.819714754601655 26.84325844703242 23.318739061453265 86-87 23.031568385672387 26.800184585728417 26.888605784731322 23.279641243867875 88-89 22.967640352939974 26.822320147501372 26.597346474860355 23.6126930246983 90-91 23.05346456633872 26.610225295141337 26.856740483528046 23.47956965499189 92-93 23.22533051230003 26.59553278224205 27.020528293428907 23.15860841202901 94-95 22.959208295264837 26.586546287064127 27.000712429564764 23.45353298810627 96-97 22.73244238569667 26.555328884275188 27.25466847791636 23.45756025211178 98-99 22.75637765571471 26.667314764998 26.795534611586852 23.780772967700432 100-101 22.681145130755937 26.467818256054766 26.97486724756172 23.876169365627586 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 136.0 1 122.0 2 142.5 3 176.5 4 180.5 5 206.5 6 323.5 7 631.0 8 1067.0 9 1389.5 10 1407.5 11 1359.5 12 1481.0 13 1685.5 14 1916.0 15 2078.0 16 2277.5 17 2508.5 18 2774.0 19 3078.0 20 3475.0 21 4140.5 22 4998.5 23 6359.0 24 8034.0 25 10185.0 26 13290.0 27 17259.0 28 21328.0 29 26301.5 30 33123.5 31 40845.5 32 49327.0 33 56270.0 34 62909.0 35 71030.5 36 80051.5 37 87779.5 38 92856.5 39 97472.0 40 102995.0 41 111183.0 42 119315.5 43 125932.5 44 131245.0 45 136129.5 46 139925.0 47 148939.5 48 166384.0 49 165388.0 50 149174.5 51 139888.0 52 133943.5 53 130844.0 54 128167.0 55 121013.0 56 111287.0 57 101247.5 58 92223.5 59 81052.0 60 67349.0 61 54774.0 62 44773.0 63 35789.5 64 27582.5 65 21794.0 66 16706.0 67 13308.5 68 10082.0 69 6822.0 70 4940.0 71 3773.0 72 3022.5 73 2492.5 74 1985.5 75 1508.0 76 1028.0 77 664.0 78 427.0 79 280.0 80 200.5 81 141.5 82 98.5 83 66.5 84 40.5 85 35.0 86 29.5 87 18.5 88 12.0 89 7.0 90 5.5 91 6.0 92 5.0 93 4.0 94 1.5 95 2.5 96 3.0 97 2.5 98 1.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013713314838079284 2 0.006297764865996649 3 0.008669650335008374 4 0.03492396880234506 5 0.04479319339754327 6 0.059242610622557236 7 0.026772316443327754 8 0.02483663979620324 9 0.014585732481853714 10-11 0.045120350013958685 12-13 0.04247583403126745 14-15 0.01894782070072585 16-17 0.022778279417922948 18-19 0.02975762056811837 20-21 0.023936959101060862 22-23 0.013822367043551088 24-25 0.003244303112786153 26-27 0.0027944627652149636 28-29 0.0014858362995533222 30-31 0.006761236739251815 32-33 0.0015948885050251257 34-35 0.0018675190187046342 36-37 0.006106923506420994 38-39 0.006325027917364601 40-41 0.0037214065117252932 42-43 8.178915410385259E-4 44-45 0.001417678671133445 46-47 0.002589989879955332 48-49 7.224708612506979E-4 50-51 0.0018266244416527081 52-53 0.0010359959519821327 54-55 0.0023173593662758236 56-57 0.0023446224176437744 58-59 0.002453674623115578 60-61 7.906284896705752E-4 62-63 0.0013358895170295926 64-65 0.0036123543062534895 66-67 0.0026308844570072584 68-69 0.006747605213567839 70-71 0.010482643250977109 72-73 0.00408945770519263 74-75 0.003366986843941932 76-77 0.002862620393634841 78-79 0.0347876535455053 80-81 0.0228600685720268 82-83 0.0037895641401451702 84-85 0.008696913386376326 86-87 0.00805623167922948 88-89 0.005602557056113903 90-91 0.01214568938442211 92-93 0.014108629082914572 94-95 0.006461343174204355 96-97 0.004103089230876605 98-99 0.02260106958403127 100-101 0.0424485709798995 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3667968.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.7354516258416 #Duplication Level Percentage of deduplicated Percentage of total 1 73.20008337112142 37.87039372254225 2 14.16265773045446 14.654229878145566 3 5.2586400348594315 8.161743514235521 4 2.5233501458660053 5.221866376260442 5 1.3645767837179286 3.5298498091892707 6 0.8374198007691118 2.5994574955927376 7 0.5173139811293112 1.8734430712260903 8 0.3587534363972063 1.4848216843465685 9 0.28489733547150703 1.3265363085855586 >10 1.3428421622713422 12.328061981883003 >50 0.09721272044089427 3.4987583198027314 >100 0.04802257243637413 4.476814537441913 >500 0.0026965772454181592 0.9544852966562999 >1k 0.0013218515685846388 1.2688447515821728 >5k 2.1149625097354212E-4 0.7506932525098535 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9054 0.24683966708542712 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7322 0.19962006211613625 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5603 0.15275487681462868 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5464 0.1489653126744835 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4454 0.12142963079285315 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 5.452610273590173E-5 0.0 0.0 0.0 5 0.0 8.17891541038526E-5 0.0 0.0 0.0 6 2.7263051367950864E-5 8.17891541038526E-5 0.0 0.0 0.0 7 2.7263051367950864E-5 1.0905220547180346E-4 0.0 0.0 0.0 8 2.7263051367950864E-5 1.0905220547180346E-4 0.0 0.0 0.0 9 2.7263051367950864E-5 1.0905220547180346E-4 0.0 0.0 0.0 10-11 2.7263051367950864E-5 1.0905220547180346E-4 0.0 0.0 0.0 12-13 2.7263051367950864E-5 1.0905220547180346E-4 0.0 0.0 0.0 14-15 2.7263051367950864E-5 1.4994678252372976E-4 0.0 5.452610273590173E-5 0.0 16-17 2.7263051367950864E-5 1.635783082077052E-4 0.0 5.452610273590173E-5 0.0 18-19 2.7263051367950864E-5 1.635783082077052E-4 0.0 5.452610273590173E-5 0.0 20-21 2.7263051367950864E-5 1.635783082077052E-4 0.0 6.815762841987716E-5 0.0 22-23 2.7263051367950864E-5 1.635783082077052E-4 0.0 1.9084135957565606E-4 0.0 24-25 2.7263051367950864E-5 1.635783082077052E-4 0.0 3.271566164154104E-4 0.0 26-27 2.7263051367950864E-5 1.635783082077052E-4 0.0 3.5441966778336124E-4 0.0 28-29 2.7263051367950864E-5 1.635783082077052E-4 0.0 5.997871300949191E-4 0.0 30-31 2.7263051367950864E-5 1.635783082077052E-4 0.0 0.0014313101968174203 0.0 32-33 2.7263051367950864E-5 1.635783082077052E-4 0.0 0.0021537810580681185 0.0 34-35 2.7263051367950864E-5 1.635783082077052E-4 0.0 0.0029989356504745955 0.0 36-37 2.7263051367950864E-5 1.635783082077052E-4 0.0 0.004362088218872138 0.0 38-39 2.7263051367950864E-5 1.635783082077052E-4 0.0 0.006556763853992183 0.0 40-41 2.7263051367950864E-5 1.635783082077052E-4 0.0 0.01056443240508096 0.0 42-43 2.7263051367950864E-5 1.635783082077052E-4 0.0 0.015117361983528754 0.0 44-45 2.7263051367950864E-5 1.635783082077052E-4 0.0 0.02095165497627024 0.0 46-47 4.0894577051926295E-5 1.635783082077052E-4 0.0 0.03195229620323842 0.0 48-49 5.452610273590173E-5 1.635783082077052E-4 0.0 0.03988584415131212 0.0 50-51 5.452610273590173E-5 1.635783082077052E-4 0.0 0.050368487402289226 0.0 52-53 5.452610273590173E-5 1.635783082077052E-4 0.0 0.06371375104690116 0.0 54-55 5.452610273590173E-5 1.635783082077052E-4 0.0 0.08088947340871022 0.0 56-57 5.452610273590173E-5 1.635783082077052E-4 0.0 0.12192036571747628 0.0 58-59 5.452610273590173E-5 1.635783082077052E-4 0.0 0.16263773293551087 0.0 60-61 5.452610273590173E-5 1.635783082077052E-4 0.0 0.19371761149497485 0.0 62-63 5.452610273590173E-5 1.635783082077052E-4 0.0 0.2322539346035734 0.0 64-65 5.452610273590173E-5 1.635783082077052E-4 0.0 0.2731485116554997 0.0 66-67 5.452610273590173E-5 1.635783082077052E-4 0.0 0.34126524549832493 0.0 68-69 5.452610273590173E-5 1.635783082077052E-4 0.0 0.42245461247208266 2.7263051367950864E-5 70-71 5.452610273590173E-5 1.635783082077052E-4 0.0 0.4668524916247906 2.7263051367950864E-5 72-73 5.452610273590173E-5 1.635783082077052E-4 0.0 0.5175345041178113 2.7263051367950864E-5 74-75 5.452610273590173E-5 1.635783082077052E-4 0.0 0.6054442132537688 2.7263051367950864E-5 76-77 5.452610273590173E-5 1.635783082077052E-4 0.0 0.7095890694793412 2.7263051367950864E-5 78-79 5.452610273590173E-5 1.635783082077052E-4 0.0 0.8129841917922949 2.7263051367950864E-5 80-81 5.452610273590173E-5 1.635783082077052E-4 0.0 0.906619141715522 2.7263051367950864E-5 82-83 5.452610273590173E-5 1.635783082077052E-4 0.0 1.039785516122278 2.7263051367950864E-5 84-85 5.452610273590173E-5 1.635783082077052E-4 0.0 1.2341847039014517 2.7263051367950864E-5 86-87 5.452610273590173E-5 1.635783082077052E-4 0.0 1.452084641959799 2.7263051367950864E-5 88-89 5.452610273590173E-5 1.635783082077052E-4 0.0 1.7213890633724178 2.7263051367950864E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12565 0.0 23.941727 1 TACATGG 12800 0.0 23.05506 2 ACATGGG 12600 0.0 22.32728 3 TAGATCG 195 4.206031E-7 21.93422 5 GAGTACT 8575 0.0 21.7751 12-13 CATGGGG 7695 0.0 20.81162 4 GTATCAA 21295 0.0 20.487045 1 GTACTTT 9560 0.0 19.578297 14-15 GTATAGC 1305 0.0 19.300991 1 AGAGTAC 15150 0.0 19.288689 10-11 ATCAACG 23710 0.0 18.178598 3 CAACGCA 23710 0.0 18.159794 5 TCAACGC 23760 0.0 18.120342 4 TATCAAC 24225 0.0 17.909836 2 AACGCAG 24100 0.0 17.884422 6 AGTACTT 9565 0.0 17.459913 12-13 GGTATCA 16900 0.0 17.266167 1 TAGCGCG 250 4.546633E-6 17.107523 4 GTAAGGT 1370 0.0 16.996525 4 TAGCCCT 2480 0.0 16.670637 4 >>END_MODULE