FastQCFastQC Report
Thu 26 May 2016
SRR936726_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936726_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3667968
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103290.2816000575795645No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79790.21753188686487995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67390.18372570316862089No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT48060.13102622487437185No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT41340.11270545435510888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39910.10880683800949191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG123150.021.2469221
GAGTACT106450.020.65513412-13
TACATGG126550.020.2687932
ACATGGG124900.019.964193
AGAGTAC172200.019.44300710-11
GTATCAA235800.019.322191
GTACTTT113800.019.17489214-15
AGTACTT113450.018.71094312-13
TAAGGTG15250.017.7503365
ACCGTCG2755.927359E-717.2693028
ATCAACG264400.017.1732033
TCAACGC264650.017.1380984
CAACGCA265500.017.0820665
TATCAAC271000.016.84422
TGCACCG10450.016.8146175
AACGCAG270550.016.7634476
CATGGGG75800.016.353194
TACTTTT129550.016.27553414-15
ACTTTTT136600.016.11400816-17
GGTATCA189850.016.0834941
Produced by FastQC (version 0.11.5)