##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936726_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3667968 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42025175792155 31.0 31.0 34.0 30.0 34.0 2 31.655924206536152 31.0 31.0 34.0 30.0 34.0 3 31.936265256403544 31.0 31.0 34.0 30.0 34.0 4 35.742490665131214 37.0 35.0 37.0 35.0 37.0 5 35.532914409286015 37.0 35.0 37.0 33.0 37.0 6 35.626168767012146 37.0 35.0 37.0 33.0 37.0 7 35.55780775622906 37.0 35.0 37.0 33.0 37.0 8 35.52994300931742 37.0 35.0 37.0 33.0 37.0 9 37.158123789520516 39.0 37.0 39.0 33.0 39.0 10-11 37.04470745110099 39.0 37.0 39.0 33.0 39.0 12-13 36.914072314698494 39.0 37.0 39.0 33.0 39.0 14-15 38.04687949840347 40.0 37.0 41.0 33.0 41.0 16-17 37.9583864962835 40.0 37.0 41.0 33.0 41.0 18-19 37.99017276050391 40.0 37.0 41.0 33.0 41.0 20-21 38.05170328639726 40.0 37.0 41.0 33.0 41.0 22-23 37.96443085108703 40.0 37.0 41.0 33.0 41.0 24-25 37.86161697702924 40.0 37.0 41.0 32.5 41.0 26-27 37.65707388941234 39.0 37.0 41.0 32.0 41.0 28-29 37.58855488924658 39.0 37.0 41.0 32.0 41.0 30-31 37.61403520968558 39.0 37.0 41.0 32.0 41.0 32-33 37.48137511014272 39.0 36.0 41.0 32.0 41.0 34-35 37.33201870899637 39.0 36.0 41.0 31.5 41.0 36-37 37.12529430463952 39.0 36.0 41.0 31.0 41.0 38-39 36.98518389473409 39.0 36.0 41.0 31.0 41.0 40-41 36.77495005408989 39.0 35.5 41.0 30.5 41.0 42-43 36.55684564314629 39.0 35.0 40.0 30.0 41.0 44-45 36.33429817272125 39.0 35.0 40.0 30.0 41.0 46-47 36.020552660219494 38.5 35.0 40.0 29.5 41.0 48-49 35.635162983973686 38.0 35.0 40.0 28.0 41.0 50-51 35.46325799461718 38.0 34.5 40.0 27.5 41.0 52-53 35.195303503193045 38.0 34.0 40.0 27.0 41.0 54-55 34.918588711788104 38.0 34.0 40.0 26.5 41.0 56-57 34.638882209441306 38.0 33.5 40.0 26.0 41.0 58-59 34.266250550713636 37.0 33.0 40.0 25.5 41.0 60-61 33.8781728739182 37.0 33.0 39.5 25.0 41.0 62-63 33.58414604489461 36.0 32.0 39.0 24.0 40.5 64-65 33.14849475240787 36.0 32.0 39.0 23.0 40.0 66-67 32.7362583315885 35.0 31.0 38.5 22.0 40.0 68-69 32.272396324068254 35.0 31.0 38.0 22.0 40.0 70-71 31.79191680516297 35.0 31.0 37.5 20.5 39.5 72-73 31.36249184834764 34.0 30.0 37.0 20.0 39.0 74-75 30.983440422599106 34.0 30.0 36.0 18.5 39.0 76-77 29.54468427750733 32.5 28.5 35.0 17.5 37.0 78-79 30.366572309245882 34.0 30.0 35.0 18.0 37.0 80-81 30.216306821651663 34.0 30.0 35.0 17.5 37.0 82-83 29.870291262082986 34.0 29.5 35.0 13.5 36.5 84-85 29.415148387335986 34.0 29.0 35.0 7.5 36.0 86-87 29.029153471349805 34.0 29.0 35.0 7.0 36.0 88-89 28.610101969264726 33.0 29.0 35.0 2.0 35.0 90-91 28.144824736747978 33.0 27.5 35.0 2.0 35.0 92-93 27.664381068755233 33.0 27.0 35.0 2.0 35.0 94-95 27.108037611015146 32.5 25.5 34.0 2.0 35.0 96-97 26.39949735112193 32.0 25.0 34.0 2.0 35.0 98-99 25.49594707478364 32.0 23.5 34.0 2.0 35.0 100-101 22.64450316905709 28.5 8.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4154903899276974 1101 2 0.41689269132959694 1101 3 0.22295728868542142 1101 4 0.16095266985860235 1101 5 0.2404421062777331 1101 6 0.2333784714645546 1101 7 0.25263439211004624 1101 8 0.22041966164815108 1101 9 0.3065036384163946 1101 10-11 0.25109425803754704 1101 12-13 0.2509899978601524 1101 14-15 0.3558444193696957 1101 16-17 0.2863424124440286 1101 18-19 0.33695278011427376 1101 20-21 0.20632577748480685 1101 22-23 0.19487911648493395 1101 24-25 0.05806885766945413 1101 26-27 0.24991095294627286 1101 28-29 0.23491512441308515 1101 30-31 0.034248719287198526 1101 32-33 0.15407157160934304 1101 34-35 0.227990750431168 1101 36-37 0.09056352273178447 1101 38-39 0.10260578570344592 1101 40-41 0.2996820361614638 1101 42-43 0.33717287907493443 1101 44-45 0.2311052222245067 1101 46-47 0.27556281086149426 1101 48-49 0.2965683827189878 1101 50-51 0.16654729824378478 1101 52-53 0.16935659057637764 1101 54-55 0.01828226068332839 1101 56-57 0.11927466089345984 1101 58-59 0.12171928331431303 1101 60-61 0.21318906784885883 1101 62-63 0.05614926805009901 1101 64-65 0.25303070731670374 1101 66-67 0.06484410405405328 1101 68-69 -0.07732193251158748 1101 70-71 0.018408122688882145 1101 72-73 -7.992723285710213E-5 1101 74-75 0.06770959534768295 1101 76-77 0.43328640985729905 1101 78-79 0.3343702988574364 1101 80-81 0.2831284838439281 1101 82-83 0.3294114590945405 1101 84-85 0.36644877774699225 1101 86-87 0.2647112755008756 1101 88-89 0.11595031821250856 1101 90-91 -0.013641738636067657 1101 92-93 0.2254424662407608 1101 94-95 0.03523495643483443 1101 96-97 -0.022692913345892407 1101 98-99 0.007862632790516244 1101 100-101 0.071486632826808 1102 1 0.41331732391539333 1102 2 0.47282685261794555 1102 3 0.18715250742774714 1102 4 0.07801016948693729 1102 5 0.12894490007416692 1102 6 0.15932995287575125 1102 7 0.14916501596580645 1102 8 -0.009942514273589609 1102 9 0.013523945464093856 1102 10-11 0.10841957872011676 1102 12-13 0.14287159281152384 1102 14-15 0.22822202332056207 1102 16-17 0.30033591054107234 1102 18-19 0.16811061137145344 1102 20-21 0.2412238771278865 1102 22-23 0.13057864813759323 1102 24-25 0.09279752955676202 1102 26-27 -0.14196770125868596 1102 28-29 -0.09032199437519495 1102 30-31 0.044843850075004354 1102 32-33 0.2662814283973205 1102 34-35 0.33696788254655274 1102 36-37 0.2694256588515387 1102 38-39 0.17017528675078353 1102 40-41 0.31364033265054303 1102 42-43 0.0982338852376401 1102 44-45 0.09042701855353386 1102 46-47 0.18130210548182646 1102 48-49 0.1520143335179185 1102 50-51 0.14114990586477205 1102 52-53 0.004845966591339845 1102 54-55 0.1947121374788665 1102 56-57 0.04550801721508435 1102 58-59 0.0887964335342204 1102 60-61 0.20981312009142528 1102 62-63 0.10509941867163519 1102 64-65 0.0023711794235410366 1102 66-67 0.046651598601584965 1102 68-69 0.04926366117357617 1102 70-71 -0.025276606935108248 1102 72-73 0.04483950825331107 1102 74-75 -0.4755261317978743 1102 76-77 0.04425591222069514 1102 78-79 0.3190320352802587 1102 80-81 0.28634967846130266 1102 82-83 0.3282346073225426 1102 84-85 0.4276483309165897 1102 86-87 0.21892513508145584 1102 88-89 0.004938276933280861 1102 90-91 0.11611199976595898 1102 92-93 0.16115434320505528 1102 94-95 0.06914021932853487 1102 96-97 0.019069645431912363 1102 98-99 -0.31622727462022837 1102 100-101 -0.4981177773843761 1103 1 0.4680107422686177 1103 2 0.4472265135870934 1103 3 0.2862517538054661 1103 4 0.15647330202524046 1103 5 0.012254026853078415 1103 6 0.06281922351985259 1103 7 0.0374314515055616 1103 8 0.24525565489578582 1103 9 7.340362733998518E-4 1103 10-11 0.12403967987948761 1103 12-13 0.156440629335016 1103 14-15 0.23211948912324942 1103 16-17 0.15504059524529623 1103 18-19 0.212097509479797 1103 20-21 0.16751410438865122 1103 22-23 0.16516263026736766 1103 24-25 0.17892649652268489 1103 26-27 0.1409362312922795 1103 28-29 0.2766546960533134 1103 30-31 0.2795300581922291 1103 32-33 0.18448986366465192 1103 34-35 0.12343254790306446 1103 36-37 0.17945741555688954 1103 38-39 0.25054403882035814 1103 40-41 0.3431684897538929 1103 42-43 0.4063505223227466 1103 44-45 0.43774595568906705 1103 46-47 0.3176270960946326 1103 48-49 0.4865484487546823 1103 50-51 0.1760708210537274 1103 52-53 0.320380275430999 1103 54-55 0.2904216755167468 1103 56-57 0.2232431354143145 1103 58-59 0.32533976042766 1103 60-61 0.3363521902362123 1103 62-63 0.15003658289965216 1103 64-65 0.28903030508497096 1103 66-67 0.2503300193324023 1103 68-69 0.2030776468021429 1103 70-71 0.20388677418885592 1103 72-73 0.1676264433788468 1103 74-75 0.2182294974478367 1103 76-77 0.0717565548485588 1103 78-79 0.21531492826655807 1103 80-81 0.17770464322666157 1103 82-83 0.2445245047667406 1103 84-85 0.39913660330826417 1103 86-87 0.4604470630530635 1103 88-89 0.23125800477116698 1103 90-91 -0.06236920694882997 1103 92-93 0.1851447829831514 1103 94-95 0.1673835145492859 1103 96-97 -0.11654180961760474 1103 98-99 -0.17287024612916468 1103 100-101 0.2015860393363873 1104 1 0.261885490624028 1104 2 0.36815712553726243 1104 3 0.07383485808734136 1104 4 0.03132690632906332 1104 5 -0.035072205495026765 1104 6 0.0497576630013441 1104 7 -0.04387702459073495 1104 8 0.008889426184872207 1104 9 -0.009384678239221955 1104 10-11 0.024217399518718707 1104 12-13 -0.03677433270072328 1104 14-15 0.09442933629672012 1104 16-17 -0.01148962116069896 1104 18-19 0.07722266707455816 1104 20-21 -0.04207096089909612 1104 22-23 0.04526266073326468 1104 24-25 0.07612133768878948 1104 26-27 0.10051416483916142 1104 28-29 0.029332915471577792 1104 30-31 -0.00284241792368789 1104 32-33 0.047601502376004134 1104 34-35 0.061210560433977435 1104 36-37 0.08698171974970847 1104 38-39 0.05583545066658502 1104 40-41 0.08634553098627862 1104 42-43 0.13603258262919837 1104 44-45 0.23773288454527375 1104 46-47 0.2098135886665915 1104 48-49 0.2055273260983128 1104 50-51 0.23428942250597373 1104 52-53 0.14297050476772455 1104 54-55 0.3080534283002976 1104 56-57 0.16265167005774828 1104 58-59 0.20519630880325934 1104 60-61 0.010976486784691986 1104 62-63 -0.0650882849681409 1104 64-65 -0.02307540157806187 1104 66-67 0.049428600717710935 1104 68-69 0.12598907191786424 1104 70-71 -0.00953240970375191 1104 72-73 0.08441117378678697 1104 74-75 0.05443500012079383 1104 76-77 0.18284679075985721 1104 78-79 0.23350501835604476 1104 80-81 0.2987790656169409 1104 82-83 0.1965082486320462 1104 84-85 0.3276751112544716 1104 86-87 0.3256408079368782 1104 88-89 0.28468569215041484 1104 90-91 0.24288568258385013 1104 92-93 0.22452820609841595 1104 94-95 0.34154513940946885 1104 96-97 -0.021108837172647554 1104 98-99 0.27927730490903 1104 100-101 0.2789704143161913 1105 1 0.3405912525028718 1105 2 0.34856034475207665 1105 3 0.1414587035618453 1105 4 0.08777131666133187 1105 5 -0.0606237761281605 1105 6 0.004303631041778999 1105 7 0.03696002840961654 1105 8 -4.795162734225755E-4 1105 9 -0.04537557161718553 1105 10-11 0.007578659427586842 1105 12-13 0.029751222524787124 1105 14-15 0.115802968293238 1105 16-17 0.09416138203632585 1105 18-19 -0.010497929613862311 1105 20-21 0.07634843479705467 1105 22-23 0.014049105661932515 1105 24-25 -0.112054274453115 1105 26-27 -0.0582369540512957 1105 28-29 -0.0740298861304538 1105 30-31 -0.024627804931860453 1105 32-33 0.08991715873526118 1105 34-35 -0.02883561726343231 1105 36-37 0.05463997089817241 1105 38-39 0.05195737799568434 1105 40-41 0.047121899753967966 1105 42-43 0.13652565863267085 1105 44-45 -0.10221660506790187 1105 46-47 0.18559530019852133 1105 48-49 0.17034914424630898 1105 50-51 -0.03304573760085816 1105 52-53 -0.0193564119323284 1105 54-55 0.07885922546531532 1105 56-57 0.12045205601265252 1105 58-59 0.03402359531031607 1105 60-61 0.02626034771598995 1105 62-63 -0.06237429031488517 1105 64-65 -0.07266941130269089 1105 66-67 0.029588438414634766 1105 68-69 -0.21488451987501378 1105 70-71 -0.15709487319652382 1105 72-73 -0.20582020644724608 1105 74-75 -0.25510600393346294 1105 76-77 -0.3459150900792203 1105 78-79 -0.16349899669141266 1105 80-81 -0.34285660105570415 1105 82-83 -0.13213081175912933 1105 84-85 -0.0674402466010875 1105 86-87 -0.2289684392982103 1105 88-89 -0.1648977018206388 1105 90-91 -0.3586517576340107 1105 92-93 -0.11875685629031807 1105 94-95 0.022775324166772748 1105 96-97 -0.06226215988690598 1105 98-99 -0.013058536333787174 1105 100-101 0.22579304783379328 1106 1 0.3369590334421382 1106 2 0.4065279718307302 1106 3 0.11904430204648975 1106 4 -0.03823602754634692 1106 5 -0.12333583302684303 1106 6 -0.008746635273631398 1106 7 -0.02761596595513538 1106 8 0.024060608829579166 1106 9 0.006500162279671429 1106 10-11 -0.09134075033174582 1106 12-13 0.047056316081643956 1106 14-15 0.03731867782658327 1106 16-17 0.1082572718083128 1106 18-19 -0.09363682464320533 1106 20-21 0.00239148940337941 1106 22-23 -0.0029276144706287255 1106 24-25 0.006560104618600349 1106 26-27 -0.03296741067794784 1106 28-29 0.04398909429288267 1106 30-31 -0.03242384295355549 1106 32-33 0.047430741530995135 1106 34-35 -0.17658684236840116 1106 36-37 0.018056247674216763 1106 38-39 -0.015629663777197322 1106 40-41 0.012512895663412849 1106 42-43 -0.15233724318112962 1106 44-45 -0.05112099043329721 1106 46-47 0.0474649263158895 1106 48-49 -0.06678961963647367 1106 50-51 -0.01597488262665081 1106 52-53 -0.03602427852246848 1106 54-55 -0.07755823568589904 1106 56-57 0.026418531840782578 1106 58-59 -0.27893960050164424 1106 60-61 -0.018901898838194597 1106 62-63 -0.29319892166797246 1106 64-65 -0.07848253353061097 1106 66-67 0.018499117463157688 1106 68-69 -0.05951741427295332 1106 70-71 -0.1656346947695937 1106 72-73 -0.012486761571729232 1106 74-75 0.01653765800314133 1106 76-77 0.06688418942588115 1106 78-79 0.03481184906197754 1106 80-81 -0.05161776625914527 1106 82-83 0.09803774875858906 1106 84-85 0.23921963289279446 1106 86-87 0.040449787099969825 1106 88-89 0.026487810945535983 1106 90-91 -0.008008260644633225 1106 92-93 0.33835255509820783 1106 94-95 0.17642552517930454 1106 96-97 0.1640891229495942 1106 98-99 0.09266359451397932 1106 100-101 0.2755330869479202 1107 1 0.531375023656917 1107 2 0.57130489825796 1107 3 0.3425158546458391 1107 4 0.21552068954880355 1107 5 0.030671357167300073 1107 6 0.1788460306676427 1107 7 0.08125257246869921 1107 8 0.19191590351675814 1107 9 0.1708756943737626 1107 10-11 0.23675377993065894 1107 12-13 0.2598328722244858 1107 14-15 0.46391017980016613 1107 16-17 0.4767213352544175 1107 18-19 0.34063978537298567 1107 20-21 0.3608459239381645 1107 22-23 0.3172260990719238 1107 24-25 0.30257457757797823 1107 26-27 0.30627681463098355 1107 28-29 0.363918398810803 1107 30-31 0.3561529978833491 1107 32-33 0.42260355355249146 1107 34-35 0.3845550372497186 1107 36-37 0.49010148458791747 1107 38-39 0.5869396772835458 1107 40-41 0.709545157448396 1107 42-43 0.5003942490497195 1107 44-45 0.3639947310217977 1107 46-47 0.47154240225119537 1107 48-49 0.6389961116675806 1107 50-51 0.4928280972520369 1107 52-53 0.32935363917423643 1107 54-55 0.43565021772428025 1107 56-57 0.46702108619594895 1107 58-59 0.36911458545699105 1107 60-61 0.41916955694089353 1107 62-63 0.3497298987486559 1107 64-65 0.47662363489111215 1107 66-67 0.41091936594825285 1107 68-69 0.41484115151246215 1107 70-71 0.43936122299692215 1107 72-73 0.5465237065496673 1107 74-75 0.6958749931393307 1107 76-77 0.5162923793878385 1107 78-79 0.5944261159368516 1107 80-81 0.7038974222292609 1107 82-83 0.5947820158003942 1107 84-85 0.7325814508069719 1107 86-87 0.8999543724042347 1107 88-89 0.9638386159346624 1107 90-91 0.8111730770228469 1107 92-93 1.018628500423592 1107 94-95 1.1412261255256126 1107 96-97 1.10634847225068 1107 98-99 1.2948481585117122 1107 100-101 1.162695373180778 1108 1 0.234454009367834 1108 2 0.31251625586625664 1108 3 0.07955943909616536 1108 4 0.07537847714770152 1108 5 -0.09959261773715866 1108 6 -0.059698763654196796 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-2.608366634848057 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 14.0 4 42.0 5 94.0 6 228.0 7 623.0 8 1496.0 9 2614.0 10 4335.0 11 6614.0 12 9289.0 13 12439.0 14 16044.0 15 20651.0 16 24602.0 17 29138.0 18 26869.0 19 21592.0 20 21481.0 21 22831.0 22 24679.0 23 28481.0 24 33565.0 25 40738.0 26 51096.0 27 63327.0 28 78160.0 29 97055.0 30 119118.0 31 146098.0 32 180104.0 33 226630.0 34 292106.0 35 389203.0 36 542220.0 37 696912.0 38 415383.0 39 22089.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.10734429494565 19.351936532832344 15.069842596861086 23.470876575360926 2 15.72536271953216 20.518615585460896 39.050358111579 24.70566358342795 3 19.80413920135972 23.591522895548813 29.634040376693555 26.970297526397914 4 13.554864688297199 16.84192530562787 36.49773198979586 33.105478016279065 5 14.24396286990508 37.07554155325237 33.94031790898939 14.740177667853155 6 32.85047743055556 35.955275509491905 16.782343793620882 14.411903266331658 7 29.19831906930486 32.20428858703238 20.20712830646287 18.390264037199888 8 26.559010329424904 34.67254894262982 19.25556602456728 19.512874703378 9 25.973057565529388 17.05533532179878 20.179888871318656 36.79171824135317 10-11 25.414123189167388 26.249682895985384 26.99460640320418 21.341587511643052 12-13 26.372347553100056 23.347148924162834 27.48314286629548 22.79736065644163 14-15 22.859017777154968 25.08281156368407 25.873304169122612 26.18486649003835 16-17 22.696844683486876 28.291154666561972 26.400462054194584 22.61153859575656 18-19 22.499558679295824 27.13820392356257 28.362301465321053 21.999935931820552 20-21 23.309298678355248 26.070991632286816 28.396816493057802 22.22289319630013 22-23 22.845571866207965 25.997415699076026 28.424052183303516 22.732960251412486 24-25 23.075392151730878 26.18260301071329 28.4063137955402 22.335691042015636 26-27 22.841434276416805 26.360467157837803 28.485444802135678 22.312653763609717 28-29 22.47901290305695 26.8619300931742 28.296334646321885 22.362722357446955 30-31 22.760697852420993 26.17395055981276 28.3199619407751 22.745389646991146 32-33 22.558604706261807 26.408430304087 28.36917505801478 22.66378993163642 34-35 23.213585147222684 26.362867293338756 28.038205023136918 22.385342536301643 36-37 23.201285219835572 26.112060045994184 28.2070719807626 22.479582753407644 38-39 22.85378092793764 26.485347994335818 28.177213940602435 22.4836571371241 40-41 23.080050051304084 26.386045288550893 27.987834245065173 22.546070415079843 42-43 22.94900022221828 26.487451483477457 27.669298294645085 22.894249999659174 44-45 23.13196231167219 26.39514781455083 27.62156704989513 22.851322823881855 46-47 23.36996557701441 26.722444299159896 27.245441394498975 22.662148729326713 48-49 23.437807591990627 26.72661864618898 27.16577480326382 22.669798958556573 50-51 22.96696438965515 26.825358354023816 27.367922853931255 22.83975440238978 52-53 23.43762971501513 26.954618266900386 26.867483856210228 22.74026816187425 54-55 23.142194580622643 26.58900419350089 27.134431968764144 23.13436925711232 56-57 23.266951106099327 26.903000955068695 26.942520568875228 22.88752736995675 58-59 23.684681179733545 26.65678002722529 26.81658479903967 22.841953994001493 60-61 23.33654829912405 26.69197742207911 27.436304484297903 22.535169794498934 62-63 23.068943076042654 26.754494423531344 27.34215909546344 22.83440340496256 64-65 22.92360892115235 27.009795881527765 27.112282543059873 22.954312654260015 66-67 23.120550843414225 26.90987586665685 26.825970669305832 23.143602620623092 68-69 23.016130500949032 26.665354401549855 27.156458716266652 23.162056381234464 70-71 23.21939663798006 26.62916851376559 27.00544505678159 23.145989791472754 72-73 23.260266073786095 26.53935728710791 27.18805441978425 23.012322219321735 74-75 23.01279618579006 27.14515230231016 26.78247192996231 23.059579581937463 76-77 23.125854536284137 26.787407048944566 26.801093261176277 23.285645153595016 78-79 23.19104289517116 26.798227927000145 26.863161947328052 23.14756723050064 80-81 23.250077337153314 26.9362339662596 26.74104030843788 23.072648388149208 82-83 23.25945531640603 26.829502054131343 26.899809051811225 23.0112335776514 84-85 23.114073355073018 26.943225071162928 26.77569227077903 23.167009302985026 86-87 23.122376502184686 26.904085124366915 26.720980484380007 23.252557889068388 88-89 23.049467840831124 26.954431859955474 26.496094299673732 23.50000599953967 90-91 23.16666553042957 26.7436580926215 26.707566657349524 23.38210971959941 92-93 23.27814811931915 26.655457588967735 26.86815651794827 23.198237773764845 94-95 23.03362783070357 26.65599047240319 26.86496559183556 23.445416105057678 96-97 22.851105767271022 26.601876203199044 27.11231002900697 23.434708000522964 98-99 22.792326018124996 26.76240171502384 26.63895544414414 23.806316822707025 100-101 22.55609043180464 26.674982924405217 26.747975127572758 24.020951516217384 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 231.0 1 181.0 2 156.0 3 197.0 4 194.0 5 195.5 6 334.0 7 688.0 8 1132.5 9 1421.5 10 1464.0 11 1442.5 12 1523.5 13 1756.5 14 2008.5 15 2240.0 16 2576.5 17 2859.5 18 3153.0 19 3601.0 20 4135.0 21 4910.5 22 5883.0 23 7145.0 24 8984.0 25 11301.0 26 14384.5 27 18415.5 28 22670.0 29 27949.5 30 34789.5 31 42188.5 32 50113.5 33 57146.0 34 63907.0 35 71116.0 36 79971.0 37 88014.0 38 92895.5 39 97249.5 40 102569.5 41 111196.5 42 119395.0 43 125967.5 44 131183.5 45 135121.0 46 138846.5 47 148815.5 48 166602.5 49 165332.5 50 148660.0 51 138758.5 52 132599.0 53 129442.5 54 127010.5 55 120120.0 56 110093.5 57 100228.5 58 91176.0 59 80250.5 60 66872.0 61 54291.0 62 44683.5 63 35564.0 64 26859.5 65 20908.0 66 16030.0 67 12853.0 68 9626.0 69 6322.0 70 4663.5 71 3643.5 72 2974.0 73 2444.0 74 1884.0 75 1506.5 76 1081.5 77 674.0 78 441.5 79 303.0 80 191.5 81 132.5 82 100.0 83 63.0 84 37.5 85 22.0 86 9.5 87 4.0 88 2.0 89 1.5 90 0.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0599601741345617 2 0.010796168341708543 3 0.004334825167504187 4 0.011886690396426577 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004034931602456728 10-11 2.0447288525963147E-4 12-13 2.8626203936348406E-4 14-15 5.4526102735901735E-5 16-17 0.0 18-19 1.3631525683975434E-5 20-21 1.3631525683975434E-5 22-23 0.0014040471454494696 24-25 0.0 26-27 0.0 28-29 0.0 30-31 1.3631525683975434E-5 32-33 0.004157615333612507 34-35 5.725240787269681E-4 36-37 0.003080724804578448 38-39 1.635783082077052E-4 40-41 0.0035850912548855388 42-43 0.0109870097012842 44-45 0.002589989879955332 46-47 0.0019084135957565605 48-49 0.009978276800670016 50-51 4.6347187325516465E-4 52-53 0.001949308172808487 54-55 0.010578063930764936 56-57 0.004607455681183696 58-59 0.0014994678252372975 60-61 0.00614781808347292 62-63 0.003953142448352875 64-65 0.018034508479899496 66-67 0.003762301088777219 68-69 2.7263051367950865E-4 70-71 0.0014722047738693466 72-73 1.3631525683975434E-5 74-75 0.0 76-77 0.0011723112088218872 78-79 0.010877957495812395 80-81 0.015935253524567282 82-83 0.013113527707984365 84-85 0.008942280848687884 86-87 0.01071437918760469 88-89 0.02780831239530988 90-91 0.024345904871580122 92-93 0.002930778022054718 94-95 0.00835612524427694 96-97 0.010946115124232273 98-99 0.010373591045505304 100-101 0.012350162269681744 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3667968.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.39467827310943 #Duplication Level Percentage of deduplicated Percentage of total 1 70.7256884822413 34.9347262822447 2 15.347711575809148 15.161905510311403 3 5.838736137517124 8.65207479102708 4 2.7348000984244254 5.403382840117699 5 1.524140566645103 3.7642216466214773 6 0.9265309584727185 2.745941916230138 7 0.5708686362673474 1.9738510837244045 8 0.41514104938296226 1.6404606857785962 9 0.2972065315820881 1.321237890734758 >10 1.4576522761765742 12.832952359277169 >50 0.1015991949977498 3.4666772349378254 >100 0.05520881415087488 4.852846200645072 >500 0.0031622784212809217 1.1381479245851318 >1k 0.0013869642065994009 1.4251991635173902 >5k 1.1095713652795202E-4 0.40332412876190094 >10k+ 5.547856826397601E-5 0.28305034148536995 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10329 0.2816000575795645 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7979 0.21753188686487995 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6739 0.18372570316862089 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4806 0.13102622487437185 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4134 0.11270545435510888 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3991 0.10880683800949191 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 2.7263051367950864E-5 0.0 0.0 0.0 5 0.0 5.452610273590173E-5 0.0 0.0 0.0 6 0.0 5.452610273590173E-5 0.0 0.0 0.0 7 0.0 5.452610273590173E-5 0.0 0.0 0.0 8 0.0 5.452610273590173E-5 0.0 0.0 0.0 9 0.0 5.452610273590173E-5 0.0 0.0 0.0 10-11 0.0 5.452610273590173E-5 0.0 0.0 0.0 12-13 0.0 5.452610273590173E-5 0.0 0.0 0.0 14-15 0.0 9.542067978782803E-5 0.0 0.0 0.0 16-17 0.0 1.0905220547180346E-4 0.0 0.0 0.0 18-19 0.0 1.0905220547180346E-4 0.0 0.0 0.0 20-21 0.0 1.0905220547180346E-4 0.0 1.3631525683975432E-5 0.0 22-23 0.0 1.0905220547180346E-4 0.0 1.0905220547180346E-4 0.0 24-25 0.0 1.0905220547180346E-4 0.0 2.181044109436069E-4 0.0 26-27 0.0 1.0905220547180346E-4 0.0 2.4536746231155777E-4 0.0 28-29 0.0 1.2268373115577888E-4 0.0 5.04366450307091E-4 0.0 30-31 0.0 1.3631525683975432E-4 0.0 0.0014176786711334452 0.0 32-33 0.0 1.3631525683975432E-4 0.0 0.0021946756351200443 0.0 34-35 0.0 1.3631525683975432E-4 0.0 0.0030398302275265214 0.0 36-37 0.0 1.3631525683975432E-4 0.0 0.004471140424343942 0.0 38-39 0.0 1.3631525683975432E-4 0.0 0.006570395379676158 0.0 40-41 0.0 1.3631525683975432E-4 0.0 0.010605326982132887 0.0 42-43 0.0 1.3631525683975432E-4 0.0 0.015185519611948632 0.0 44-45 0.0 1.3631525683975432E-4 0.0 0.021169759387213848 0.0 46-47 0.0 1.3631525683975432E-4 0.0 0.032306715871021775 0.0 48-49 0.0 1.3631525683975432E-4 0.0 0.04039021060161921 0.0 50-51 0.0 1.3631525683975432E-4 0.0 0.05092737995533222 0.0 52-53 0.0 1.3631525683975432E-4 0.0 0.06427264359994417 0.0 54-55 0.0 1.3631525683975432E-4 0.0 0.08142110291038526 0.0 56-57 0.0 1.3631525683975432E-4 0.0 0.12288820404103852 0.0 58-59 0.0 1.3631525683975432E-4 0.0 0.16402814855527637 0.0 60-61 0.0 1.3631525683975432E-4 0.0 0.19499897490926854 0.0 62-63 0.0 1.3631525683975432E-4 0.0 0.2338215600572306 0.0 64-65 0.0 1.3631525683975432E-4 0.0 0.2747570316862088 0.0 66-67 0.0 1.3631525683975432E-4 0.0 0.3428056079006142 0.0 68-69 0.0 1.3631525683975432E-4 0.0 0.4237905019891122 0.0 70-71 0.0 1.3631525683975432E-4 0.0 0.46822927571887213 0.0 72-73 1.3631525683975432E-5 1.3631525683975432E-4 0.0 0.5185296054927415 0.0 74-75 2.7263051367950864E-5 1.3631525683975432E-4 0.0 0.6068346288735342 0.0 76-77 2.7263051367950864E-5 1.3631525683975432E-4 0.0 0.7113339047668901 0.0 78-79 2.7263051367950864E-5 1.3631525683975432E-4 0.0 0.8149471314907872 0.0 80-81 2.7263051367950864E-5 1.3631525683975432E-4 0.0 0.9089228695561139 0.0 82-83 2.7263051367950864E-5 1.3631525683975432E-4 0.0 1.0424164005792853 0.0 84-85 2.7263051367950864E-5 1.3631525683975432E-4 0.0 1.237415375488554 0.0 86-87 2.7263051367950864E-5 1.3631525683975432E-4 0.0 1.4552471559184812 0.0 88-89 2.7263051367950864E-5 1.3631525683975432E-4 0.0 1.7236382651102735 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12315 0.0 21.246922 1 GAGTACT 10645 0.0 20.655134 12-13 TACATGG 12655 0.0 20.268793 2 ACATGGG 12490 0.0 19.96419 3 AGAGTAC 17220 0.0 19.443007 10-11 GTATCAA 23580 0.0 19.32219 1 GTACTTT 11380 0.0 19.174892 14-15 AGTACTT 11345 0.0 18.710943 12-13 TAAGGTG 1525 0.0 17.750336 5 ACCGTCG 275 5.927359E-7 17.269302 8 ATCAACG 26440 0.0 17.173203 3 TCAACGC 26465 0.0 17.138098 4 CAACGCA 26550 0.0 17.082066 5 TATCAAC 27100 0.0 16.8442 2 TGCACCG 1045 0.0 16.814617 5 AACGCAG 27055 0.0 16.763447 6 CATGGGG 7580 0.0 16.35319 4 TACTTTT 12955 0.0 16.275534 14-15 ACTTTTT 13660 0.0 16.114008 16-17 GGTATCA 18985 0.0 16.083494 1 >>END_MODULE