FastQCFastQC Report
Thu 26 May 2016
SRR936725_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936725_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3704566
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT93160.25147345195091675No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74340.2006712797126573No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT55790.15059793778812416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54860.1480875222630667No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44060.11893430971401239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG126400.025.11341
TACATGG129450.023.4574492
ACATGGG128100.022.6656513
GAGTACT92300.020.97884412-13
GTATCAA216050.020.7631781
AGAGTAC156750.019.73329710-11
GTACTTT97850.019.5944214-15
CATGGGG78650.019.3949344
GTATAGC11950.018.689861
TCAACGC243350.018.2779084
ATCAACG242850.018.2761573
GTGTAGG17950.018.2667271
CAACGCA244900.018.0838765
AGTACTT100650.017.98733112-13
TATCAAC248150.017.9818062
AACGCAG247950.017.8992736
TAGCCCT23650.017.8830184
GTGTAGC25700.017.3808421
GGTATCA172000.017.2950551
ACCGTGC5750.016.5268978
Produced by FastQC (version 0.11.5)