FastQCFastQC Report
Thu 26 May 2016
SRR936725_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936725_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3704566
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103240.27868311699670084No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81730.22061963533650097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67570.18239653443885195No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT50700.13685813668861616No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42880.11574905130587497No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41570.11221287459853596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG129200.023.2036821
TACATGG131600.021.9442372
ACATGGG130150.021.0935963
GAGTACT107600.020.15271612-13
AGAGTAC174650.019.56933410-11
GTATCAA244750.019.198341
GTACTTT114600.018.58977314-15
TAAGGTG16300.018.3570275
AGTACTT115200.018.32839212-13
CATGGGG78400.018.2347494
GTACCGT2905.226684E-818.0158926
TCAACGC275600.016.6474324
ATCAACG275900.016.647223
CAACGCA276800.016.592425
AACGCAG280000.016.4541246
TATCAAC280850.016.4385932
ATTCGCG5750.016.10759780-81
GGTATCA198100.015.9247891
TACTTTT131950.015.87542814-15
ACTTTTT138800.015.62070716-17
Produced by FastQC (version 0.11.5)