##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936725_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3704566 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.783513102479482 31.0 31.0 34.0 30.0 34.0 2 31.93790932595073 31.0 31.0 34.0 30.0 34.0 3 32.0509641345302 33.0 31.0 34.0 30.0 34.0 4 35.8019643866515 37.0 35.0 37.0 35.0 37.0 5 35.55842141832539 37.0 35.0 37.0 33.0 37.0 6 35.65002567102327 37.0 35.0 37.0 33.0 37.0 7 35.59165230151116 37.0 35.0 37.0 33.0 37.0 8 35.557888022510596 37.0 35.0 37.0 33.0 37.0 9 37.184540645246976 39.0 37.0 39.0 33.0 39.0 10-11 37.04532582224206 39.0 37.0 39.0 33.0 39.0 12-13 36.94290748227998 39.0 37.0 39.0 33.0 39.0 14-15 38.04783097399263 40.0 37.0 41.0 33.0 41.0 16-17 37.96695496854422 40.0 37.0 41.0 33.0 41.0 18-19 38.09243255485258 40.0 37.0 41.0 33.0 41.0 20-21 38.11310879601011 40.0 37.0 41.0 33.0 41.0 22-23 38.01973928929866 40.0 37.0 41.0 33.0 41.0 24-25 37.89645629204608 40.0 37.0 41.0 33.0 41.0 26-27 37.68070267880232 39.5 37.0 41.0 32.0 41.0 28-29 37.60507398167559 39.0 37.0 41.0 32.0 41.0 30-31 37.61507947219728 39.5 37.0 41.0 32.0 41.0 32-33 37.50252364244557 39.5 36.5 41.0 32.0 41.0 34-35 37.34984151989734 39.0 36.0 41.0 31.5 41.0 36-37 37.09968765572 39.0 36.0 41.0 31.0 41.0 38-39 36.951136516396254 39.0 36.0 41.0 31.0 41.0 40-41 36.75931971518391 39.0 35.0 40.5 30.0 41.0 42-43 36.53632463289897 39.0 35.0 40.0 30.0 41.0 44-45 36.33005850077985 39.0 35.0 40.0 30.0 41.0 46-47 36.047753906935384 38.5 35.0 40.0 29.5 41.0 48-49 35.752955676859315 38.0 35.0 40.0 28.5 41.0 50-51 35.45242384668001 38.0 34.5 40.0 27.5 41.0 52-53 35.174341339849256 38.0 34.0 40.0 27.0 41.0 54-55 34.90310281960154 38.0 34.0 40.0 26.5 41.0 56-57 34.57804935854834 38.0 33.5 40.0 26.0 41.0 58-59 34.230605825351745 37.0 33.0 40.0 25.5 41.0 60-61 33.854982338012064 37.0 33.0 39.5 25.0 41.0 62-63 33.45497056875219 36.0 32.0 39.0 23.5 40.0 64-65 33.03661346565293 36.0 31.5 39.0 23.0 40.0 66-67 32.56290493947199 35.0 31.0 38.5 22.0 40.0 68-69 32.25950138288803 35.0 31.0 38.0 22.0 40.0 70-71 31.81129044535851 35.0 31.0 37.5 20.5 39.5 72-73 31.347833457414445 34.5 30.0 37.0 19.5 39.0 74-75 30.807117082000968 34.0 30.0 36.0 18.0 39.0 76-77 29.381356142662867 32.5 28.0 35.0 17.0 37.0 78-79 30.18415989889234 34.0 29.5 35.0 17.5 37.0 80-81 29.98200275011972 34.0 29.0 35.0 13.5 37.0 82-83 29.63587381085936 34.0 29.0 35.0 9.5 36.5 84-85 29.211454594141394 34.0 29.0 35.0 7.0 36.0 86-87 28.81919919904248 33.0 29.0 35.0 4.5 36.0 88-89 28.405924067758544 33.0 28.5 35.0 2.0 35.0 90-91 27.899222607992407 33.0 27.0 35.0 2.0 35.0 92-93 27.375556138019945 32.5 26.0 34.0 2.0 35.0 94-95 26.74169740800947 32.0 25.0 34.0 2.0 35.0 96-97 25.91727371033476 32.0 24.5 34.0 2.0 35.0 98-99 24.901411663336543 31.5 19.5 34.0 2.0 35.0 100-101 21.619071842693586 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3250737450775141 1101 2 0.34528993955334286 1101 3 0.30046969199617735 1101 4 0.19224214362558456 1101 5 0.2500583141874273 1101 6 0.24635750994325178 1101 7 0.22102405485753707 1101 8 0.2336209654215864 1101 9 0.28044272596019226 1101 10-11 0.3368536085989078 1101 12-13 0.34140250853775456 1101 14-15 0.48039256713434497 1101 16-17 0.4183556329978657 1101 18-19 0.4311607521315608 1101 20-21 0.3626342750744982 1101 22-23 0.3866662204886211 1101 24-25 0.26798585322550394 1101 26-27 0.31889916176793065 1101 28-29 0.25309406599741635 1101 30-31 0.3545199650769817 1101 32-33 0.4170268991272934 1101 34-35 0.37345635363553953 1101 36-37 0.3302101766247034 1101 38-39 0.392436643266997 1101 40-41 0.43706742838957524 1101 42-43 0.4123333281245678 1101 44-45 0.37902303295328466 1101 46-47 0.3656288705632562 1101 48-49 0.27173415989652483 1101 50-51 0.3591776826816897 1101 52-53 0.3458118663685994 1101 54-55 0.248517828186003 1101 56-57 0.3697926293992495 1101 58-59 0.33989278638932063 1101 60-61 0.37200383911415 1101 62-63 0.2565952818632198 1101 64-65 0.13645597333374582 1101 66-67 0.06670162627959542 1101 68-69 0.13446025620373803 1101 70-71 0.20624829084290397 1101 72-73 0.26670156740145856 1101 74-75 0.17571806865588613 1101 76-77 0.03449725690905936 1101 78-79 -0.1629942676479459 1101 80-81 -0.039476178202921375 1101 82-83 0.23792128712038618 1101 84-85 0.2340931015802603 1101 86-87 0.17858348536620028 1101 88-89 0.11030838841086421 1101 90-91 0.18913341719961707 1101 92-93 0.07470093085024132 1101 94-95 0.2193769771645222 1101 96-97 0.21911579882163323 1101 98-99 0.48336638006751187 1101 100-101 0.8731844251264249 1102 1 0.20080803339171638 1102 2 0.27315502893602783 1102 3 0.21865972927565736 1102 4 0.10823621413369722 1102 5 0.09317100388713584 1102 6 0.10393581152542453 1102 7 0.1845321418625545 1102 8 0.2041000213995119 1102 9 0.2032011138428942 1102 10-11 0.2483816269157515 1102 12-13 0.17664188975711426 1102 14-15 0.33007260940399163 1102 16-17 0.30644542663981866 1102 18-19 0.31918978315864877 1102 20-21 0.12189573044186375 1102 22-23 0.1139751998528169 1102 24-25 0.17403232048120998 1102 26-27 -0.04563822044946875 1102 28-29 0.07855301453999175 1102 30-31 0.20843489741620402 1102 32-33 0.2608838574740773 1102 34-35 0.19953907298271645 1102 36-37 0.2796319043964459 1102 38-39 0.2934763591210512 1102 40-41 0.260110255759173 1102 42-43 0.2740041532632702 1102 44-45 0.3074012288613446 1102 46-47 0.3233897535291774 1102 48-49 0.2810119066896135 1102 50-51 0.23977004479164776 1102 52-53 0.13327671504914207 1102 54-55 0.19716402538563926 1102 56-57 0.20804621791599232 1102 58-59 0.05517076801375964 1102 60-61 0.2935127773828512 1102 62-63 0.16737066566572167 1102 64-65 0.3398561846819632 1102 66-67 0.19912229731021824 1102 68-69 0.18393599518867632 1102 70-71 0.3181125583158213 1102 72-73 0.3842544778426422 1102 74-75 0.3174106156955432 1102 76-77 0.08212707462121571 1102 78-79 0.08055256653176102 1102 80-81 0.4525398490375139 1102 82-83 0.20524083389586067 1102 84-85 0.06168108995610666 1102 86-87 0.17727811653419323 1102 88-89 0.13432738039724157 1102 90-91 0.249763058788254 1102 92-93 0.2559007723390714 1102 94-95 0.323869594653587 1102 96-97 0.5744646701634011 1102 98-99 0.6131636912484204 1102 100-101 0.9512263718784126 1103 1 0.17356792377800545 1103 2 0.2044544291845618 1103 3 0.2379868407522494 1103 4 0.17750567045245447 1103 5 0.014160467464471083 1103 6 0.16954209800494624 1103 7 0.17628136719315535 1103 8 0.21860787375636903 1103 9 0.155233745284022 1103 10-11 0.18748462980769176 1103 12-13 0.19149705127195205 1103 14-15 0.275345575566611 1103 16-17 0.4304023506567418 1103 18-19 0.16038938944972614 1103 20-21 0.23316984051956524 1103 22-23 0.23651371510617025 1103 24-25 0.19459115056387333 1103 26-27 0.30468950662800864 1103 28-29 0.33581032882032247 1103 30-31 0.2737508407901075 1103 32-33 0.3218226603141119 1103 34-35 0.2812389583614987 1103 36-37 0.35166283604407766 1103 38-39 0.4327390622061742 1103 40-41 0.4627058863710758 1103 42-43 0.38801251900370204 1103 44-45 0.45205985749552013 1103 46-47 0.4525023924126401 1103 48-49 0.42555321511713373 1103 50-51 0.4901088549821395 1103 52-53 0.43464751810969204 1103 54-55 0.46136683372014176 1103 56-57 0.46443497217409657 1103 58-59 0.44454481905810184 1103 60-61 0.4307619717037099 1103 62-63 0.40980434500657026 1103 64-65 0.35259883594635966 1103 66-67 0.5507669049450499 1103 68-69 0.5170896634119515 1103 70-71 0.5096601054448975 1103 72-73 0.3251001136228382 1103 74-75 0.3198062908696002 1103 76-77 0.22091435235598667 1103 78-79 0.27364095349664197 1103 80-81 0.44662801319162426 1103 82-83 0.6809944855806052 1103 84-85 0.4166044040021468 1103 86-87 0.23328929841899182 1103 88-89 0.2958211428442077 1103 90-91 0.1876310123976701 1103 92-93 0.29523060578227955 1103 94-95 0.578788544364965 1103 96-97 0.657852519396311 1103 98-99 0.8459233634179775 1103 100-101 1.114803874422357 1104 1 0.16164832670164486 1104 2 0.24617787653377476 1104 3 0.17527420791442694 1104 4 0.144822097885573 1104 5 0.046917500305703186 1104 6 0.17324755241611456 1104 7 0.18757141028643787 1104 8 0.13438257285615407 1104 9 0.19145880756617117 1104 10-11 0.15069540625381705 1104 12-13 0.13475859086976527 1104 14-15 0.13123367505897932 1104 16-17 0.2371097623041507 1104 18-19 0.11777490249275502 1104 20-21 0.07376289162075977 1104 22-23 0.09555938489853588 1104 24-25 0.15720791007380086 1104 26-27 0.1775073586677678 1104 28-29 0.1867942874371309 1104 30-31 0.07797369869589232 1104 32-33 0.05394053763602358 1104 34-35 0.018908853000262127 1104 36-37 0.15032271156498922 1104 38-39 0.15272269982487785 1104 40-41 0.17015923511989683 1104 42-43 0.09750122299515596 1104 44-45 0.14200557188674168 1104 46-47 0.0025149198608218626 1104 48-49 -0.015795516656275765 1104 50-51 -0.12798171761598098 1104 52-53 -0.12089884623168246 1104 54-55 -0.07082583900098172 1104 56-57 0.04221866490220805 1104 58-59 -0.14167544245592723 1104 60-61 -0.026539740949012014 1104 62-63 -0.10322175810483003 1104 64-65 0.09977393899243481 1104 66-67 -0.08764548955878126 1104 68-69 0.06097203607087209 1104 70-71 -0.17449806992233619 1104 72-73 -0.1223360773511466 1104 74-75 0.02823703623856133 1104 76-77 -0.1186751393591372 1104 78-79 -0.18866602863825577 1104 80-81 -0.001749383996656917 1104 82-83 -0.037228093937439866 1104 84-85 -0.07680536286232709 1104 86-87 -0.03751336728563004 1104 88-89 -0.00955656966079843 1104 90-91 0.06696330793181815 1104 92-93 0.07120653944645738 1104 94-95 0.10633526663343318 1104 96-97 0.3492453955109269 1104 98-99 0.47790662038406495 1104 100-101 0.9519385544327008 1105 1 0.10550494244624176 1105 2 0.14944148262642543 1105 3 0.14403199809149214 1105 4 0.0896637069930506 1105 5 -0.006801479602955851 1105 6 -0.012183860277659164 1105 7 0.05495644189711868 1105 8 0.07770281944197421 1105 9 -0.04283878282237907 1105 10-11 -0.024100839837032595 1105 12-13 -0.07900763632216723 1105 14-15 0.12486482564282397 1105 16-17 0.2544470685340414 1105 18-19 0.09405311024580953 1105 20-21 0.20064195544959773 1105 22-23 0.12075017392817244 1105 24-25 0.11894767878710155 1105 26-27 0.23836115644940037 1105 28-29 0.14640759092218758 1105 30-31 0.19959163691444104 1105 32-33 0.11610198835261087 1105 34-35 0.20257532655303123 1105 36-37 0.17134727452761922 1105 38-39 0.2830588586207483 1105 40-41 0.22291282366354892 1105 42-43 0.1536577408703792 1105 44-45 0.2336617579060487 1105 46-47 0.21662489916200656 1105 48-49 0.2050094870560102 1105 50-51 0.24183207746580138 1105 52-53 0.3778058188750819 1105 54-55 0.3812567537499305 1105 56-57 0.27358065221293515 1105 58-59 0.4347419197957336 1105 60-61 0.20343191068101163 1105 62-63 0.31507565229406254 1105 64-65 0.17334673355674823 1105 66-67 0.5168735162233844 1105 68-69 0.5204767832979087 1105 70-71 0.33796185425702774 1105 72-73 0.4508833187835748 1105 74-75 0.1180378101940569 1105 76-77 0.007180402072229697 1105 78-79 0.314912728083236 1105 80-81 0.5209232654509997 1105 82-83 0.434333175664559 1105 84-85 0.3646228550151456 1105 86-87 0.3898054056349274 1105 88-89 0.4156805104570971 1105 90-91 0.4156813597688398 1105 92-93 0.29435225231796736 1105 94-95 0.32434851837145473 1105 96-97 0.49336021203397706 1105 98-99 0.6097526673613594 1105 100-101 0.9550458226397751 1106 1 0.07266786609971376 1106 2 0.12375340191228545 1106 3 0.13892638676306746 1106 4 0.13796840923885867 1106 5 0.015548891051089697 1106 6 0.04191385014716076 1106 7 0.0395899138268021 1106 8 0.017399047250599153 1106 9 -0.010302150531252607 1106 10-11 0.06462059746180415 1106 12-13 0.08910961892306091 1106 14-15 0.17313653147207475 1106 16-17 0.16793986745080502 1106 18-19 0.14566075948764023 1106 20-21 0.10827256199097235 1106 22-23 0.06267260556460741 1106 24-25 -0.06806290286164796 1106 26-27 0.02979263104158747 1106 28-29 0.01202920009871633 1106 30-31 0.0846778265178969 1106 32-33 0.1042670456603716 1106 34-35 0.11339000180902303 1106 36-37 0.14980676928922065 1106 38-39 0.3178799941077912 1106 40-41 0.1804962436196007 1106 42-43 0.17504198328632725 1106 44-45 0.12494967814020441 1106 46-47 0.011276573259998202 1106 48-49 0.04734611205259398 1106 50-51 0.07471548556821972 1106 52-53 -0.058842030146088575 1106 54-55 0.09460645421768987 1106 56-57 0.19559598671339273 1106 58-59 0.05855074345924294 1106 60-61 0.08758268891774179 1106 62-63 0.08261196544886928 1106 64-65 0.12621896049002856 1106 66-67 0.12165594503318289 1106 68-69 0.24849889032711303 1106 70-71 0.20265984871317144 1106 72-73 0.017512942840983925 1106 74-75 0.11310783782214529 1106 76-77 -0.03359957850604545 1106 78-79 0.033174392515949336 1106 80-81 0.09527217035749302 1106 82-83 0.2508540084280071 1106 84-85 0.10126836750249169 1106 86-87 0.18024875437798826 1106 88-89 0.12298497107111572 1106 90-91 0.28362018361470476 1106 92-93 0.1901844458787103 1106 94-95 0.4747932576370779 1106 96-97 0.4064951223566666 1106 98-99 0.7396071297990154 1106 100-101 0.9683322087400228 1107 1 0.03721670481182926 1107 2 0.12236737265638098 1107 3 0.10148171278414253 1107 4 0.030025523222782624 1107 5 0.025376098563626215 1107 6 0.01950651084898425 1107 7 0.01574223611612524 1107 8 -0.12329466629265795 1107 9 -0.006596982797312023 1107 10-11 0.08040983897829079 1107 12-13 0.04630403539179895 1107 14-15 0.16066364308426984 1107 16-17 0.08870555781298606 1107 18-19 0.031365993675819936 1107 20-21 0.07893948760003155 1107 22-23 0.0014236209968885305 1107 24-25 0.03911169191069064 1107 26-27 0.057771241941686924 1107 28-29 -0.05461884792249094 1107 30-31 -0.05787179360445549 1107 32-33 -0.04882582643469391 1107 34-35 0.06393108829006167 1107 36-37 0.019556053473912982 1107 38-39 0.02631011575343223 1107 40-41 0.03865642355019361 1107 42-43 -0.15724206047722333 1107 44-45 -0.04632696514144641 1107 46-47 -0.032580820519200415 1107 48-49 -0.09478077888568492 1107 50-51 0.05414331454250032 1107 52-53 -0.05251375306398387 1107 54-55 0.0287452587975352 1107 56-57 -0.17717822583247766 1107 58-59 -0.1215030730193476 1107 60-61 -0.045417685746691916 1107 62-63 0.023645454949665634 1107 64-65 0.0937549746791504 1107 66-67 0.12805703534106527 1107 68-69 0.16374097281973832 1107 70-71 0.007162410065905789 1107 72-73 -0.0221747336780993 1107 74-75 -0.012650170200483757 1107 76-77 -0.08178881498117718 1107 78-79 0.021794848277473022 1107 80-81 0.3258039990751165 1107 82-83 0.2094395527970967 1107 84-85 0.15973475254066116 1107 86-87 0.23952548015455832 1107 88-89 0.15938201683632158 1107 90-91 0.23609594786713473 1107 92-93 0.17800355513979582 1107 94-95 0.4298870325932249 1107 96-97 0.5057259717908309 1107 98-99 0.8272436044702083 1107 100-101 0.9761430455937123 1108 1 -0.03567103575829478 1108 2 -0.022627057049856347 1108 3 -0.0035859001504690013 1108 4 -0.13846067668419693 1108 5 -0.08943327520625388 1108 6 0.0703506423298208 1108 7 0.06428321105873636 1108 8 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-0.9720770300456714 2316 88-89 -0.7524029097505078 2316 90-91 -1.0186390329028683 2316 92-93 -0.7364851399543291 2316 94-95 -0.8434556244858946 2316 96-97 -1.0638797040620602 2316 98-99 -1.2979557209442802 2316 100-101 -1.507959931609129 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 24.0 4 43.0 5 128.0 6 242.0 7 602.0 8 1510.0 9 2803.0 10 4306.0 11 6515.0 12 9296.0 13 12656.0 14 16403.0 15 20582.0 16 25166.0 17 30064.0 18 27611.0 19 22551.0 20 22428.0 21 24320.0 22 26174.0 23 29893.0 24 35687.0 25 43004.0 26 52618.0 27 65515.0 28 81022.0 29 100189.0 30 122081.0 31 149502.0 32 184414.0 33 231661.0 34 297443.0 35 396419.0 36 551382.0 37 699338.0 38 392912.0 39 18059.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.24005988211519 19.358562526800597 15.016812141466096 23.384565449618123 2 15.902594228012068 20.460114476563835 38.939362731110734 24.69792856431336 3 19.85129667967184 23.595118592018245 29.545006064372238 27.008578663937676 4 13.54786515543664 16.79299841383473 36.52261608213939 33.136520348589244 5 14.266367358088916 37.08332860939745 33.9106792803853 14.739624752128336 6 32.93719949165298 35.83976055430124 16.79129698673741 14.431742967308365 7 29.25989171201161 32.17915404935423 20.157502930167798 18.403451308466362 8 26.606706426609755 34.636068030641106 19.240121514908896 19.517104027840237 9 25.988903249770278 17.042263102144737 20.132686985694185 36.836146662390796 10-11 25.476643072283466 26.213114993059893 26.975608939699626 21.33463299495702 12-13 26.35913327736582 23.29974382715216 27.521925858341007 22.819197037141016 14-15 22.886867463944018 25.06467502545902 25.84911657701177 26.19934093358519 16-17 22.695012486970104 28.274392917837044 26.377787972691845 22.652806622501004 18-19 22.51049110746036 27.121719521261063 28.32902693594877 22.038762435329808 20-21 23.370119468785276 26.03655866841082 28.381381246818115 22.211940615985785 22-23 22.896049893805063 25.965148408371636 28.39777913828233 22.74102255954097 24-25 23.12736956190183 26.202910756680666 28.333893457613442 22.335826223804066 26-27 22.85580622668489 26.333795035162133 28.447275423812314 22.363123314340662 28-29 22.500408300995446 26.825388898262194 28.275309600134975 22.39889320060739 30-31 22.76007110771826 26.170148066396177 28.290624927031715 22.779155898853844 32-33 22.58373551882536 26.369619422164792 28.333015256099863 22.71362980290999 34-35 23.287274796648326 26.33736434953987 27.922198791086583 22.453162062725223 36-37 23.22218534192022 26.107658775886833 28.141701169396537 22.52845471279641 38-39 22.877750124789397 26.448927054152254 28.15742820189075 22.515894619167597 40-41 23.114281965952795 26.342932819076452 27.980237030382476 22.562548184588273 42-43 22.988573076741904 26.422453742913593 27.665468902283564 22.923504278060935 44-45 23.16161594075557 26.359644185733256 27.580248653198243 22.89849122031293 46-47 23.386178986881582 26.741514921332133 27.207318975900524 22.664987115885765 48-49 23.468565956524994 26.69453785453939 27.124078074418094 22.712818114517514 50-51 22.985222408900675 26.802321375354698 27.358438106015125 22.8540181097295 52-53 23.472320095841088 26.887320181548457 26.849392982604375 22.790966740006084 54-55 23.170312797994523 26.574055565311085 27.09928851815174 23.15634311854265 56-57 23.287723557165727 26.868120454943313 26.91506329252312 22.929092695367842 58-59 23.655147988923844 26.646792821985166 26.826478040152956 22.87158114893803 60-61 23.361667045943214 26.653396036789555 27.42828096482554 22.55665595244169 62-63 23.101268471034704 26.732374450684205 27.31500890432167 22.85134817395943 64-65 22.94099861374886 26.98535595068887 27.074024731152317 22.999620704409953 66-67 23.144617229384863 26.880980400705152 26.794611175845663 23.179791194064318 68-69 23.030382296288227 26.649697856101735 27.140486823459447 23.17943302415059 70-71 23.23817228595445 26.576772098833818 26.98995184855861 23.195103766653116 72-73 23.25728073505889 26.471368284064912 27.23855280016747 23.032798180708728 74-75 23.04333659348292 27.105610759735434 26.74253555772179 23.108517089059855 76-77 23.143033570997144 26.750331525828646 26.795952179297267 23.310682723876948 78-79 23.183946777201065 26.784138709692655 26.850545732100308 23.181368781005972 80-81 23.31022558400382 26.89870248367813 26.70019449922187 23.090877433096182 82-83 23.221514846675483 26.83295443517359 26.883286724113052 23.06224399403787 84-85 23.160288649713724 26.896759122345703 26.755560318722456 23.187391909218118 86-87 23.14062135992569 26.907226847473954 26.684682067895594 23.267469724704764 88-89 23.07628139471725 26.94800181199426 26.436340203541597 23.5393765897469 90-91 23.198239788342647 26.72904349234632 26.66418293242623 23.408533786884803 92-93 23.28375875956939 26.646461619858986 26.843491194540725 23.2262884260309 94-95 23.027087261018576 26.633836077044176 26.8786617903982 23.460414871539047 96-97 22.874366197335178 26.597718546604295 27.03108050709342 23.496834748967107 98-99 22.815501678729902 26.74688127414756 26.55924145028766 23.87837559683488 100-101 22.57742360221117 26.67024351594836 26.67511612949814 24.07721675234233 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 211.0 1 168.0 2 156.5 3 201.0 4 195.5 5 199.0 6 352.5 7 711.5 8 1136.0 9 1446.5 10 1463.0 11 1405.0 12 1547.5 13 1763.0 14 1986.5 15 2225.0 16 2492.0 17 2788.5 18 3103.0 19 3500.5 20 4013.0 21 4752.5 22 5682.5 23 7105.5 24 8981.0 25 11218.0 26 14422.0 27 18395.5 28 22673.0 29 28118.0 30 34672.5 31 41917.0 32 50272.0 33 57188.5 34 63884.5 35 71594.5 36 80349.0 37 88491.5 38 93491.5 39 97993.0 40 103527.0 41 111886.5 42 119885.5 43 126518.5 44 132228.0 45 136821.0 46 140027.0 47 149511.0 48 168393.0 49 167309.5 50 150220.0 51 140831.5 52 134488.5 53 130953.0 54 128824.5 55 121920.0 56 111730.5 57 101657.0 58 92709.5 59 81738.5 60 67879.5 61 55319.5 62 45744.0 63 36190.5 64 27389.5 65 21566.5 66 16628.0 67 13231.0 68 9772.5 69 6511.5 70 4869.0 71 3773.0 72 3029.0 73 2464.5 74 2006.5 75 1661.5 76 1147.5 77 688.0 78 445.0 79 308.0 80 203.5 81 146.5 82 97.5 83 57.5 84 40.0 85 24.5 86 12.0 87 6.5 88 3.0 89 2.5 90 2.0 91 2.5 92 2.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03482189276692601 2 0.0061275733783660484 3 0.003860101291217379 4 9.177863209887474E-4 5 7.558240290495567E-4 6 2.6993715323198454E-4 7 0.0 8 0.0 9 0.0015656354887455103 10-11 3.239245838783815E-4 12-13 9.717737516351443E-4 14-15 0.002253975229487071 16-17 0.0028208432512742384 18-19 0.0 20-21 0.0 22-23 0.0015656354887455103 24-25 8.368051750191521E-4 26-27 0.004184025875095761 28-29 0.005155799626730904 30-31 0.0012282140472055297 32-33 4.0490572984797676E-4 34-35 0.008921422914317089 36-37 0.010541045833708995 38-39 0.008772957480039497 40-41 0.0016736103500383042 42-43 6.883397407415606E-4 44-45 0.0013226920508367242 46-47 1.079748612927938E-4 48-49 6.343523100951637E-4 50-51 0.0010257611822815412 52-53 0.0027128683899814445 54-55 0.003090780404506223 56-57 0.0016331197770535063 58-59 8.637988903423504E-4 60-61 0.003914088721863776 62-63 0.0049938373347917136 64-65 0.008934919771978687 66-67 0.003279736411768612 68-69 0.0033067301270918105 70-71 1.079748612927938E-4 72-73 0.001255207762528728 74-75 0.006397510531598034 76-77 0.00279384953595104 78-79 0.003738629572262986 80-81 0.004885862473498919 82-83 0.004980340477130115 84-85 0.005790151936826068 86-87 0.003860101291217379 88-89 0.009717737516351443 90-91 0.012579071340610478 92-93 0.001241710904867129 94-95 0.0025374092403806546 96-97 0.0011202391859127358 98-99 0.0034686924190310015 100-101 0.005088315338422909 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3704566.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.81980956976046 #Duplication Level Percentage of deduplicated Percentage of total 1 70.2883541709713 34.31464065598697 2 15.54012564591028 15.173319494469812 3 5.852826568162567 8.572016355075935 4 2.8114713380274137 5.4902198133335185 5 1.6039546720054723 3.915238082291738 6 0.9545473618788112 2.796049225934447 7 0.5926328005177064 2.0252554326247783 8 0.4033946670560837 1.5754920661707943 9 0.3028900665313202 1.3308331831768017 >10 1.483783688900767 12.996577242782934 >50 0.10408156533136059 3.519734806754787 >100 0.05694688949204207 4.936750987956081 >500 0.0032161420418106304 1.1053064064606426 >1k 0.0015526202763497614 1.4276671249581248 >5k 1.6635217246604574E-4 0.5414187587538868 >10k+ 5.5450724155348566E-5 0.2794803632687563 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10324 0.27868311699670084 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8173 0.22061963533650097 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6757 0.18239653443885195 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5070 0.13685813668861616 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4288 0.11574905130587497 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4157 0.11221287459853596 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 2.699371532319845E-5 0.0 0.0 0.0 5 0.0 5.39874306463969E-5 0.0 0.0 2.699371532319845E-5 6 0.0 5.39874306463969E-5 0.0 0.0 2.699371532319845E-5 7 0.0 8.098114596959535E-5 0.0 0.0 2.699371532319845E-5 8 0.0 8.098114596959535E-5 0.0 0.0 2.699371532319845E-5 9 0.0 8.098114596959535E-5 0.0 2.699371532319845E-5 2.699371532319845E-5 10-11 0.0 8.098114596959535E-5 0.0 5.39874306463969E-5 2.699371532319845E-5 12-13 0.0 8.098114596959535E-5 0.0 5.39874306463969E-5 2.699371532319845E-5 14-15 0.0 8.098114596959535E-5 0.0 8.098114596959535E-5 2.699371532319845E-5 16-17 0.0 8.098114596959535E-5 0.0 8.098114596959535E-5 2.699371532319845E-5 18-19 0.0 8.098114596959535E-5 0.0 8.098114596959535E-5 2.699371532319845E-5 20-21 0.0 8.098114596959535E-5 0.0 8.098114596959535E-5 2.699371532319845E-5 22-23 0.0 8.098114596959535E-5 0.0 1.079748612927938E-4 2.699371532319845E-5 24-25 0.0 8.098114596959535E-5 0.0 2.4294343790878608E-4 2.699371532319845E-5 26-27 0.0 8.098114596959535E-5 0.0 3.3742144153998065E-4 2.699371532319845E-5 28-29 0.0 8.098114596959535E-5 0.0 7.018365984031598E-4 2.699371532319845E-5 30-31 0.0 8.098114596959535E-5 0.0 0.0012147171895439304 5.39874306463969E-5 32-33 0.0 8.098114596959535E-5 0.0 0.0021864909411790745 5.39874306463969E-5 34-35 0.0 8.098114596959535E-5 0.0 0.0034686924190310015 5.39874306463969E-5 36-37 0.0 8.098114596959535E-5 0.0 0.005263774488023698 5.39874306463969E-5 38-39 0.0 8.098114596959535E-5 0.0 0.007949649162681944 5.39874306463969E-5 40-41 0.0 8.098114596959535E-5 0.0 0.012660052486580075 5.39874306463969E-5 42-43 0.0 8.098114596959535E-5 0.0 0.017208493518539016 5.39874306463969E-5 44-45 0.0 8.098114596959535E-5 0.0 0.02355201661949065 5.39874306463969E-5 46-47 0.0 8.098114596959535E-5 0.0 0.03560471051129876 5.39874306463969E-5 48-49 0.0 8.098114596959535E-5 0.0 0.0436758313929351 5.39874306463969E-5 50-51 0.0 8.098114596959535E-5 0.0 0.053960436931073705 5.39874306463969E-5 52-53 0.0 8.098114596959535E-5 0.0 0.0681591311910761 5.39874306463969E-5 54-55 0.0 8.098114596959535E-5 0.0 0.08642037960721985 5.39874306463969E-5 56-57 0.0 8.098114596959535E-5 0.0 0.1270459211686335 8.098114596959535E-5 58-59 0.0 8.098114596959535E-5 0.0 0.1672665570001992 8.098114596959535E-5 60-61 0.0 8.098114596959535E-5 0.0 0.2021019466247868 8.098114596959535E-5 62-63 0.0 8.098114596959535E-5 0.0 0.24278147561684688 8.098114596959535E-5 64-65 0.0 8.098114596959535E-5 0.0 0.28512111810128365 8.098114596959535E-5 66-67 0.0 8.098114596959535E-5 0.0 0.35592563339403316 8.098114596959535E-5 68-69 0.0 8.098114596959535E-5 0.0 0.4416306795451883 8.098114596959535E-5 70-71 0.0 8.098114596959535E-5 0.0 0.48706110243413125 8.098114596959535E-5 72-73 0.0 8.098114596959535E-5 0.0 0.5379172621030373 8.098114596959535E-5 74-75 0.0 8.098114596959535E-5 0.0 0.6294799444793264 8.098114596959535E-5 76-77 0.0 8.098114596959535E-5 0.0 0.7356462268454658 8.098114596959535E-5 78-79 0.0 8.098114596959535E-5 0.0 0.8434321321309972 8.098114596959535E-5 80-81 0.0 8.098114596959535E-5 0.0 0.9423236082175348 8.098114596959535E-5 82-83 0.0 8.098114596959535E-5 0.0 1.0742688887173288 8.098114596959535E-5 84-85 0.0 8.098114596959535E-5 0.0 1.2685696516137113 8.098114596959535E-5 86-87 1.3496857661599226E-5 8.098114596959535E-5 0.0 1.489769651829661 8.098114596959535E-5 88-89 2.699371532319845E-5 8.098114596959535E-5 0.0 1.7632834723419692 8.098114596959535E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12920 0.0 23.203682 1 TACATGG 13160 0.0 21.944237 2 ACATGGG 13015 0.0 21.093596 3 GAGTACT 10760 0.0 20.152716 12-13 AGAGTAC 17465 0.0 19.569334 10-11 GTATCAA 24475 0.0 19.19834 1 GTACTTT 11460 0.0 18.589773 14-15 TAAGGTG 1630 0.0 18.357027 5 AGTACTT 11520 0.0 18.328392 12-13 CATGGGG 7840 0.0 18.234749 4 GTACCGT 290 5.226684E-8 18.015892 6 TCAACGC 27560 0.0 16.647432 4 ATCAACG 27590 0.0 16.64722 3 CAACGCA 27680 0.0 16.59242 5 AACGCAG 28000 0.0 16.454124 6 TATCAAC 28085 0.0 16.438593 2 ATTCGCG 575 0.0 16.107597 80-81 GGTATCA 19810 0.0 15.924789 1 TACTTTT 13195 0.0 15.875428 14-15 ACTTTTT 13880 0.0 15.620707 16-17 >>END_MODULE