FastQCFastQC Report
Thu 26 May 2016
SRR936724_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936724_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3755487
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT92180.2454541847701776No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73550.19584677033897332No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT55030.14653226066286476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54420.14490797065733418No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44020.11721515744828834No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37810.10067935263788691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG125300.024.8838371
TACATGG129100.023.5610792
ACATGGG127450.022.8240933
GTATCAA224900.021.3872851
GAGTACT91400.020.85299912-13
AGAGTAC154800.020.52733810-11
GTACTTT99300.019.04646914-15
CATGGGG77150.019.0375164
AGTACTT99950.018.9502912-13
TATCAAC253650.018.7934932
TCAACGC251000.018.7288174
ATCAACG250900.018.7170853
CAACGCA250700.018.7133065
AACGCAG256000.018.3815846
GTGTAGC25700.017.3843841
TAGCCCT23300.017.3400654
ACGCAGA276750.016.9003317
CGCAGAG279050.016.6435728
TACTTTT118150.016.1485114-15
GTATATG14250.016.0099871
Produced by FastQC (version 0.11.5)