FastQCFastQC Report
Thu 26 May 2016
SRR936724_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936724_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3755487
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT105650.2813217034168937No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80990.2156577828654446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69540.18516906063048547No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT51850.1380646504700988No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT43800.11662934793809697No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40120.10683035249489613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG116650.022.769121
TACATGG118900.021.575252
GAGTACT111700.021.36540812-13
ACATGGG117950.020.8199043
GTACTTT118700.019.80457114-15
AGTACTT116950.019.55349312-13
AGAGTAC173500.019.40748610-11
GTATCAA245300.017.858811
ACCGTCG2956.362461E-817.7087298
CATGGGG71850.017.1189924
GGTATCA201100.016.8932481
TACTTTT139300.016.4837614-15
ACTTTTT145500.016.48289716-17
GAGTACA94700.015.9549831
ATACCGT4852.5465852E-1115.6667036
CCTATAC8800.015.6531393
TCAACGC277400.015.6303794
CAACGCA277800.015.6249685
TAAGGTG15200.015.6218195
ATCAACG277750.015.579393
Produced by FastQC (version 0.11.5)