##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936724_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3755487 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.798662064334135 31.0 31.0 34.0 30.0 34.0 2 31.968061132950268 31.0 31.0 34.0 30.0 34.0 3 32.05490579517384 33.0 31.0 34.0 30.0 34.0 4 35.76263051902456 37.0 35.0 37.0 35.0 37.0 5 35.57367446618774 37.0 35.0 37.0 33.0 37.0 6 35.650013433677174 37.0 35.0 37.0 33.0 37.0 7 35.588557223071206 37.0 35.0 37.0 33.0 37.0 8 35.54993693228069 37.0 35.0 37.0 33.0 37.0 9 37.169751086876346 39.0 37.0 39.0 33.0 39.0 10-11 37.05621814161519 39.0 37.0 39.0 33.0 39.0 12-13 36.950728627206004 39.0 37.0 39.0 33.0 39.0 14-15 38.07817827621291 40.0 37.0 41.0 33.0 41.0 16-17 37.970440584669845 40.0 37.0 41.0 33.0 41.0 18-19 38.09187596708496 40.0 37.0 41.0 33.0 41.0 20-21 38.09935502372928 40.0 37.0 41.0 33.0 41.0 22-23 38.01320547774496 40.0 37.0 41.0 33.0 41.0 24-25 37.88082930389587 40.0 37.0 41.0 33.0 41.0 26-27 37.68054728987212 39.5 37.0 41.0 32.0 41.0 28-29 37.60621911352642 39.0 37.0 41.0 32.0 41.0 30-31 37.617880583796456 39.5 37.0 41.0 32.0 41.0 32-33 37.491642362228916 39.5 36.5 41.0 32.0 41.0 34-35 37.335399643242006 39.0 36.0 41.0 31.5 41.0 36-37 37.14820368170626 39.0 36.0 41.0 31.0 41.0 38-39 36.997516433953834 39.0 36.0 41.0 31.0 41.0 40-41 36.823000452404706 39.0 35.5 41.0 30.5 41.0 42-43 36.57149445597868 39.0 35.0 40.0 30.0 41.0 44-45 36.34205230906138 39.0 35.0 40.0 30.0 41.0 46-47 36.044380795353575 38.5 35.0 40.0 29.5 41.0 48-49 35.72301062418802 38.0 35.0 40.0 28.0 41.0 50-51 35.44999463451744 38.0 34.0 40.0 27.5 41.0 52-53 35.09103892517801 38.0 34.0 40.0 27.0 41.0 54-55 34.9018203231698 38.0 34.0 40.0 26.0 41.0 56-57 34.598209499859806 38.0 33.5 40.0 26.0 41.0 58-59 34.22267578079754 37.0 33.0 40.0 25.5 41.0 60-61 33.79021841907588 37.0 32.5 39.5 24.0 41.0 62-63 33.456350401425965 36.0 32.0 39.0 23.5 40.0 64-65 33.03330260496175 36.0 31.5 39.0 23.0 40.0 66-67 32.60245968099477 35.0 31.0 38.5 22.0 40.0 68-69 32.235513529936334 35.0 31.0 38.0 22.0 40.0 70-71 31.855357906977176 35.0 31.0 37.5 20.5 39.5 72-73 31.386572367312148 34.5 30.0 37.0 19.5 39.0 74-75 30.87475259533584 34.0 30.0 36.0 18.0 39.0 76-77 29.372897309989355 32.5 28.0 35.0 16.5 37.0 78-79 30.16365214418263 34.0 29.0 35.0 17.5 37.0 80-81 29.934534588989393 34.0 29.0 35.0 13.0 37.0 82-83 29.6350067248269 34.0 29.0 35.0 11.0 36.5 84-85 29.255684682173044 34.0 29.0 35.0 7.0 36.0 86-87 28.870045482782928 33.5 29.0 35.0 4.5 36.0 88-89 28.40241971280955 33.0 28.0 35.0 2.0 35.0 90-91 27.9164257525056 33.0 27.0 35.0 2.0 35.0 92-93 27.280363505452158 32.5 26.0 34.0 2.0 35.0 94-95 26.711269670218535 32.0 25.0 34.0 2.0 35.0 96-97 25.95283141707054 32.0 24.5 34.0 2.0 35.0 98-99 25.03330912874948 32.0 20.5 34.0 2.0 35.0 100-101 21.784806737448434 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.37366489041761497 1101 2 0.41983630596041976 1101 3 0.3778323729457469 1101 4 0.3160465181924863 1101 5 0.30828428207855296 1101 6 0.2997565380401923 1101 7 0.33492434756744416 1101 8 0.2567246231059528 1101 9 0.2395909967819918 1101 10-11 0.41243284696449933 1101 12-13 0.39264708218489375 1101 14-15 0.6014711200633229 1101 16-17 0.6206451949479543 1101 18-19 0.5280725879259052 1101 20-21 0.4605978734606424 1101 22-23 0.45903556320025274 1101 24-25 0.49311368329168914 1101 26-27 0.5565474588250936 1101 28-29 0.46185557033344793 1101 30-31 0.6013687668397409 1101 32-33 0.5072032005998608 1101 34-35 0.499966876363402 1101 36-37 0.5892868582516542 1101 38-39 0.4434831252941578 1101 40-41 0.6247228372312321 1101 42-43 0.6181143472432709 1101 44-45 0.5468952469126833 1101 46-47 0.5543029452994617 1101 48-49 0.5279947811892711 1101 50-51 0.4489718007624717 1101 52-53 0.5769742359035206 1101 54-55 0.552540049037411 1101 56-57 0.5315409528130317 1101 58-59 0.46811705934307923 1101 60-61 0.5692006869878483 1101 62-63 0.5006359903256765 1101 64-65 0.41554093714561446 1101 66-67 0.37832284779967296 1101 68-69 0.35624296608137485 1101 70-71 0.3894804530782672 1101 72-73 0.5209940634646308 1101 74-75 0.5975455273580685 1101 76-77 0.39980478365839645 1101 78-79 0.4490221075335086 1101 80-81 0.5672620831224151 1101 82-83 0.7198468037456074 1101 84-85 0.7085325801732267 1101 86-87 0.7159286710432298 1101 88-89 0.7171813094514192 1101 90-91 0.8498120641681233 1101 92-93 0.7656619830175835 1101 94-95 0.7335978683276956 1101 96-97 0.8272572577703841 1101 98-99 0.7235285437317636 1101 100-101 1.0825261061823568 1102 1 0.28588147178401613 1102 2 0.2687370265029081 1102 3 0.2751666241132469 1102 4 0.2156790289596202 1102 5 0.2224679726489711 1102 6 0.20517043218158193 1102 7 0.23107195673033232 1102 8 0.25800525303602484 1102 9 0.3262105296300035 1102 10-11 0.3129853845555175 1102 12-13 0.29392290012281563 1102 14-15 0.4084205137878669 1102 16-17 0.41170268829827705 1102 18-19 0.3118542543306333 1102 20-21 0.3401660955385921 1102 22-23 0.3393946824113172 1102 24-25 0.3496139142910195 1102 26-27 0.39174686137952364 1102 28-29 0.3295131636689064 1102 30-31 0.4790300296155223 1102 32-33 0.50674926143909 1102 34-35 0.34574436510558826 1102 36-37 0.4352780421131257 1102 38-39 0.34474069084807013 1102 40-41 0.3361464828865621 1102 42-43 0.4126282969240549 1102 44-45 0.45488183828471307 1102 46-47 0.36792198533310483 1102 48-49 0.5325447212527123 1102 50-51 0.5010755420982136 1102 52-53 0.5810691108474302 1102 54-55 0.48738431234867363 1102 56-57 0.42874400015521275 1102 58-59 0.5678617156020991 1102 60-61 0.7209612005388237 1102 62-63 0.658091781036731 1102 64-65 0.5386778606043663 1102 66-67 0.33017104390707885 1102 68-69 0.41201958487227586 1102 70-71 0.477627200823445 1102 72-73 0.5598853306944669 1102 74-75 0.508168106694896 1102 76-77 0.3911138636031275 1102 78-79 0.5488434475097606 1102 80-81 0.5219202574525923 1102 82-83 0.512795433714551 1102 84-85 0.5336331401421432 1102 86-87 0.545623794598832 1102 88-89 0.5380230785862601 1102 90-91 0.4450910494872815 1102 92-93 0.5059386773069825 1102 94-95 0.3729837988686029 1102 96-97 0.6299254600077475 1102 98-99 0.766822191167293 1102 100-101 1.5026443697785474 1103 1 0.1252810880598254 1103 2 0.1671855188426825 1103 3 0.14731133206810654 1103 4 0.16161994106701627 1103 5 0.20929054078448672 1103 6 0.12533012756630768 1103 7 0.09657138631546758 1103 8 0.04657139870877103 1103 9 0.19440042658594336 1103 10-11 0.10780753782287178 1103 12-13 0.15869313599856838 1103 14-15 0.15537592515666887 1103 16-17 0.1818173588284253 1103 18-19 0.10826826505417841 1103 20-21 0.018395548107697834 1103 22-23 0.026768665052188112 1103 24-25 0.05384272412614877 1103 26-27 -0.02021754283966004 1103 28-29 -0.014660668631314877 1103 30-31 0.09673077692075083 1103 32-33 0.006461601042730081 1103 34-35 -0.06867430059112678 1103 36-37 -0.04568555210886416 1103 38-39 -0.0055649714097683045 1103 40-41 0.11639474696803376 1103 42-43 0.059728756314939346 1103 44-45 0.1317712516779821 1103 46-47 0.12872391025673835 1103 48-49 0.09584362960921311 1103 50-51 0.1215582955761434 1103 52-53 0.06435398710208773 1103 54-55 0.005329063083202357 1103 56-57 -0.1517906083802032 1103 58-59 0.029340069561285986 1103 60-61 -0.019113592318561246 1103 62-63 0.12997014917115735 1103 64-65 -0.008946441989436948 1103 66-67 -0.15294685772344963 1103 68-69 -0.10542816673043376 1103 70-71 -0.1645659765229972 1103 72-73 -0.21585378164234115 1103 74-75 -0.24056299005192017 1103 76-77 -0.18191695842339684 1103 78-79 -0.2816203425452457 1103 80-81 -0.15656874744444593 1103 82-83 -0.17248749910093153 1103 84-85 0.13069915993460768 1103 86-87 0.13344000892568175 1103 88-89 -0.03295755709011772 1103 90-91 -1.782125333598117E-4 1103 92-93 0.1088759069688372 1103 94-95 0.06184589306630173 1103 96-97 0.17690968358959935 1103 98-99 0.05914339008780445 1103 100-101 0.4876063800214254 1104 1 0.246182187418178 1104 2 0.22816909517279882 1104 3 0.2001418157417092 1104 4 0.17779989355614134 1104 5 0.16386007087256615 1104 6 0.1375516632001066 1104 7 0.1280326324740173 1104 8 0.12413201976529109 1104 9 0.1765177048985862 1104 10-11 0.22945706155613266 1104 12-13 0.2173216373962461 1104 14-15 0.2810382899814172 1104 16-17 0.3721487825187779 1104 18-19 0.16429629176933247 1104 20-21 0.16984684144456708 1104 22-23 0.1341403367129388 1104 24-25 0.24341355439548096 1104 26-27 0.2780578902283679 1104 28-29 0.23985240717696144 1104 30-31 0.19147580147726728 1104 32-33 0.12892305811271854 1104 34-35 0.050594912860610464 1104 36-37 0.1506201722384617 1104 38-39 0.20543504434830595 1104 40-41 0.16234638291683723 1104 42-43 0.11350806107434153 1104 44-45 0.15704588377603557 1104 46-47 0.11506311854757456 1104 48-49 0.3043634872212735 1104 50-51 0.12640238906604395 1104 52-53 0.37136483252609764 1104 54-55 0.34790341301079053 1104 56-57 0.2645087577807388 1104 58-59 0.33119990357213425 1104 60-61 0.4058694817720294 1104 62-63 0.26653157714198983 1104 64-65 0.3764555198290296 1104 66-67 0.33208344908960186 1104 68-69 0.26195889625010693 1104 70-71 0.3417410942704002 1104 72-73 0.39698950252187615 1104 74-75 0.35530711048403063 1104 76-77 0.31533967873978597 1104 78-79 0.3371012638316486 1104 80-81 0.5470201790376379 1104 82-83 0.4505401831236675 1104 84-85 0.6598512875353038 1104 86-87 0.6789151454054583 1104 88-89 0.5178589214133744 1104 90-91 0.6247600099301458 1104 92-93 0.6613969211449877 1104 94-95 0.7214657847797348 1104 96-97 0.775873497725307 1104 98-99 0.8027858065373508 1104 100-101 1.3620622007481593 1105 1 -0.0028279043461694187 1105 2 0.07224282722888731 1105 3 0.05928992437336689 1105 4 0.1419307745892482 1105 5 0.1283543329701189 1105 6 0.0992129448786443 1105 7 0.02209421040284809 1105 8 0.1054159341575982 1105 9 0.0873160766818799 1105 10-11 0.10130260157137627 1105 12-13 0.11886423116377642 1105 14-15 0.16633870570724696 1105 16-17 0.15565919715043464 1105 18-19 0.09265206121838077 1105 20-21 0.1768861275275384 1105 22-23 0.20569033894712874 1105 24-25 0.16971566021057072 1105 26-27 0.14051674888042953 1105 28-29 0.07893278409685678 1105 30-31 0.1288283591546957 1105 32-33 0.19166116619259554 1105 34-35 0.31662255025789676 1105 36-37 0.1755485054174173 1105 38-39 0.13309630802073258 1105 40-41 0.29002635969226986 1105 42-43 0.29111772286814386 1105 44-45 0.3467221829959044 1105 46-47 0.3223926731376068 1105 48-49 0.3589319560514497 1105 50-51 0.38862217512357944 1105 52-53 0.4711893502370188 1105 54-55 0.36742361523602796 1105 56-57 0.21666885909218792 1105 58-59 0.26841771917526813 1105 60-61 0.43204305375596874 1105 62-63 0.4545826035982117 1105 64-65 0.468419899095025 1105 66-67 0.34313210586384457 1105 68-69 0.2982301425676184 1105 70-71 0.4125260023406234 1105 72-73 0.5071838299219742 1105 74-75 0.3902718088338375 1105 76-77 0.2063910320125153 1105 78-79 0.36930693939206805 1105 80-81 0.4423056756482069 1105 82-83 0.550911042801296 1105 84-85 0.5304449607279693 1105 86-87 0.7763616961050168 1105 88-89 0.5588023954147836 1105 90-91 0.7165369543820042 1105 92-93 0.5143898383901551 1105 94-95 0.7188667733816985 1105 96-97 0.8171748570599107 1105 98-99 0.6382858664380571 1105 100-101 1.13720471555391 1106 1 0.06587894446272813 1106 2 0.1407242470281922 1106 3 0.0788546821358409 1106 4 0.11407133161605998 1106 5 0.10690688471627396 1106 6 -0.007075841036773056 1106 7 -0.0237071219322047 1106 8 0.08201745018697437 1106 9 0.12738313437142068 1106 10-11 0.11068874349663105 1106 12-13 0.16001296227954498 1106 14-15 0.129933160792973 1106 16-17 0.0962004770051692 1106 18-19 0.13243851766394954 1106 20-21 -0.0165275533346545 1106 22-23 -0.04517929892900696 1106 24-25 -0.07993536576149296 1106 26-27 -0.16409105865766094 1106 28-29 -0.2582408343815743 1106 30-31 -0.21992294050693317 1106 32-33 -0.07726020946009982 1106 34-35 -0.024843616871308427 1106 36-37 -0.34082610115660117 1106 38-39 0.004460727849142643 1106 40-41 0.023165206765632718 1106 42-43 0.07891106167791406 1106 44-45 -0.039758850322172634 1106 46-47 0.0038409514552384394 1106 48-49 -0.20414790358068302 1106 50-51 0.0014341821009651312 1106 52-53 -0.1787082090888461 1106 54-55 -0.08559700672315529 1106 56-57 -0.28628588688844303 1106 58-59 -0.18055215292655902 1106 60-61 -0.15206492310189645 1106 62-63 -0.14422998629780182 1106 64-65 -0.19070644278631477 1106 66-67 -0.16770899016428586 1106 68-69 -0.15254098448482978 1106 70-71 0.0582729947097711 1106 72-73 0.12820500790263978 1106 74-75 -0.01681010137552974 1106 76-77 -0.26396532881147294 1106 78-79 -0.265331694047358 1106 80-81 -0.16671895861664865 1106 82-83 -0.05096636195852966 1106 84-85 0.2895738710669704 1106 86-87 0.383756269185465 1106 88-89 0.20891430520966736 1106 90-91 0.2648877289759213 1106 92-93 0.20412503040496333 1106 94-95 0.08245510495569519 1106 96-97 0.26647857354099 1106 98-99 0.44324521095002467 1106 100-101 1.0717814138886688 1107 1 0.0472522113142837 1107 2 0.06932825276658505 1107 3 0.06046456696233804 1107 4 0.11035271775502054 1107 5 0.01308019697086138 1107 6 -0.028116336482227666 1107 7 0.026482107709590252 1107 8 0.12944759339642076 1107 9 0.006775079045326038 1107 10-11 0.10533098639766081 1107 12-13 0.054111420820795786 1107 14-15 0.07177080633514521 1107 16-17 0.16332728632885107 1107 18-19 -0.009984193604722691 1107 20-21 0.004139241081986711 1107 22-23 -0.014487476739695637 1107 24-25 0.10360047393128013 1107 26-27 0.08167665715563288 1107 28-29 0.11675878797915828 1107 30-31 -0.0025948332694625265 1107 32-33 0.06718497587662142 1107 34-35 0.06899960607914579 1107 36-37 0.18466937774608994 1107 38-39 0.046252608907010995 1107 40-41 0.06740052177720202 1107 42-43 0.09404179931314616 1107 44-45 0.2398097917646993 1107 46-47 0.15144570966684512 1107 48-49 0.11138737202691829 1107 50-51 0.2952027349786661 1107 52-53 0.37492902890503643 1107 54-55 0.10668172148273669 1107 56-57 0.06877500258797653 1107 58-59 0.14423763147809865 1107 60-61 0.2163947154606376 1107 62-63 0.17561768696955937 1107 64-65 0.20075941150275867 1107 66-67 0.23839542989833262 1107 68-69 0.2556593065347741 1107 70-71 0.19701612383304834 1107 72-73 0.43836361747370134 1107 74-75 0.35629199156602454 1107 76-77 0.18664603679253844 1107 78-79 0.28903301204571363 1107 80-81 0.45550873757665755 1107 82-83 0.399680205020001 1107 84-85 0.669051836571029 1107 86-87 0.5777827119185197 1107 88-89 0.4042468133472532 1107 90-91 0.49121789316052755 1107 92-93 0.7258669907886635 1107 94-95 0.7342983514276789 1107 96-97 0.9115481584312732 1107 98-99 0.7274800717155081 1107 100-101 1.1850873824017505 1108 1 0.15585578333821104 1108 2 0.15075661573217403 1108 3 0.08543691665289543 1108 4 0.10898329767800874 1108 5 0.16792148549999553 1108 6 0.11387130068683149 1108 7 0.08656278201107881 1108 8 0.15351345843046715 1108 9 0.15669844251389975 1108 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12 9500.0 13 12731.0 14 16393.0 15 20862.0 16 25001.0 17 30574.0 18 28101.0 19 22944.0 20 22846.0 21 24435.0 22 26576.0 23 30041.0 24 36003.0 25 43206.0 26 53829.0 27 66012.0 28 82004.0 29 101377.0 30 124087.0 31 150946.0 32 186234.0 33 234052.0 34 301723.0 35 403278.0 36 558890.0 37 707803.0 38 400181.0 39 19411.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.16136418959696 19.3526874767297 15.043840048407553 23.442108285265792 2 15.932346024889545 20.463623072231883 38.86508352885094 24.738947374027628 3 19.82912342334761 23.514663226730097 29.54330474649464 27.11290860342765 4 13.488157461335906 16.79430657062586 36.52556912059608 33.19196684744215 5 14.267329116067822 37.023813454703095 33.960961357591664 14.747896071637415 6 32.983418661814035 35.819295872945375 16.765548649216466 14.431736816024127 7 29.22158963671023 32.141051213863875 20.19130940940549 18.446049740020403 8 26.567845927838384 34.67190806412058 19.218625973142764 19.54162003489827 9 26.037874715050275 17.02173379910515 20.113503255370077 36.8268882304745 10-11 25.4853897776773 26.22657194659441 26.94883513110284 21.33920314462545 12-13 26.377174622012827 23.318940478927765 27.482088372063917 22.821796526995488 14-15 22.889134958895408 25.073449322929513 25.814552891434268 26.222862826740812 16-17 22.73504560264403 28.247637321847197 26.347935445870625 22.66938162963815 18-19 22.543312966815904 27.145410969746862 28.26987652673989 22.04139953669734 20-21 23.368833389650927 26.042481308016775 28.35729427368541 22.231391028646886 22-23 22.863521337925903 25.983558180043083 28.361973002418196 22.790947479612818 24-25 23.12283333692807 26.204124791272076 28.333449163850123 22.33959270794973 26-27 22.853044027610707 26.340609690907364 28.39289268665152 22.413453594830415 28-29 22.506922413975598 26.858034227487625 28.216953653211856 22.418089705324917 30-31 22.78835217116977 26.21660185605934 28.227197448387724 22.767848524383165 32-33 22.57614853436399 26.392859182915597 28.323690340012213 22.707301942708195 34-35 23.280714711099517 26.333755789466395 27.948954837136036 22.43657466229805 36-37 23.19574198236619 26.063475904993172 28.165710347064334 22.5750717655763 38-39 22.865834621130634 26.469829728499445 28.10837122166796 22.555964428701962 40-41 23.08186662132848 26.385079086417836 27.95348025372519 22.57957403852849 42-43 22.96710627051281 26.46808822256692 27.618549906464906 22.94625560045536 44-45 23.180415423076674 26.3384738529559 27.55905490843978 22.922055815527646 46-47 23.4077072950176 26.715326212541086 27.173563120143594 22.703403372297718 48-49 23.458867268174377 26.712430354588474 27.082439077905935 22.74626329933121 50-51 23.00778315228918 26.786803882908817 27.32411152918126 22.881301435620742 52-53 23.511118489960733 26.910671968964806 26.794321948587907 22.783887592486547 54-55 23.16889890819078 26.582367129304913 27.081366175482994 23.167367787021313 56-57 23.27563753273752 26.8779897871189 26.877443828887635 22.968928851255942 58-59 23.667593660713788 26.604311867397147 26.81291882200995 22.915175649879117 60-61 23.403017416737256 26.690060859436286 27.355438364123497 22.55148335970296 62-63 23.149109855505984 26.749209565324843 27.25472290044784 22.846957678721328 64-65 22.993629385789696 26.962332944491497 27.040607338990185 23.003430330728627 66-67 23.155945385882497 26.832870617670824 26.782449433690353 23.228734562756333 68-69 23.05422363197387 26.66084221003779 27.098888036493303 23.18604612149504 70-71 23.270518962471808 26.590863732992037 26.943148598575924 23.19546870596023 72-73 23.28577136410955 26.489524060331465 27.16665337500215 23.05805120055684 74-75 23.09804334566974 27.08315497978852 26.706605434205617 23.11219624033612 76-77 23.115033346665253 26.766843354552567 26.796028010879276 23.322095287902904 78-79 23.228118315764355 26.780776244812394 26.768379783888808 23.22272565553444 80-81 23.341257866107487 26.882742349083994 26.663928834473143 23.11207095033538 82-83 23.265796927370953 26.801224577303774 26.855169364793802 23.07780913053147 84-85 23.180375848386383 26.868168060744747 26.724427481526014 23.22702860934286 86-87 23.153980291085183 26.86796347960028 26.6886450573787 23.289411171935832 88-89 23.082577708173137 26.895531727599064 26.45958517014503 23.56230539408277 90-91 23.206405121795697 26.689357267334874 26.634757565468693 23.469480045400736 92-93 23.3270957241502 26.63346363903567 26.7647104652397 23.27473017157443 94-95 23.059978379426546 26.618476520688507 26.811562065788923 23.50998303409602 96-97 22.881907951262203 26.543809801641377 27.038833852174804 23.535448394921616 98-99 22.8460372765991 26.70103323498836 26.563812957345423 23.889116531067117 100-101 22.6370528654843 26.617757356712463 26.65210133739031 24.093088440412927 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 234.0 1 178.0 2 144.0 3 192.5 4 208.0 5 214.5 6 348.0 7 687.5 8 1137.5 9 1409.5 10 1420.0 11 1422.0 12 1520.0 13 1663.0 14 1920.0 15 2219.0 16 2496.0 17 2815.5 18 3194.0 19 3576.5 20 4074.0 21 4885.0 22 5889.5 23 7110.0 24 8917.0 25 11345.0 26 14387.0 27 18395.5 28 22863.5 29 28096.0 30 34939.5 31 42596.5 32 50861.5 33 57736.5 34 64444.0 35 72356.5 36 81192.0 37 89194.0 38 94218.0 39 98739.5 40 104655.0 41 113187.0 42 121587.5 43 128670.0 44 133939.0 45 138402.0 46 141836.5 47 151511.5 48 170350.5 49 169744.5 50 152802.5 51 142744.0 52 136735.0 53 133306.0 54 130974.5 55 124299.0 56 114218.0 57 103944.0 58 94290.5 59 82876.0 60 69213.5 61 56298.0 62 46272.0 63 36885.5 64 27910.0 65 21808.0 66 16761.5 67 13475.5 68 10033.0 69 6632.0 70 4856.0 71 3732.0 72 3037.0 73 2506.0 74 2016.0 75 1600.0 76 1122.5 77 716.5 78 439.0 79 301.0 80 222.5 81 163.0 82 111.5 83 70.5 84 44.5 85 24.0 86 17.0 87 10.5 88 5.0 89 4.0 90 3.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018666021211097256 2 0.013233969389322878 3 0.010091900198296519 4 0.0 5 0.00348822935613943 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.452835278087768E-4 14-15 1.730800825565366E-4 16-17 7.988311502609383E-5 18-19 0.0015577207430088294 20-21 0.0 22-23 6.656926252174484E-5 24-25 0.0 26-27 0.0015843484480175273 28-29 0.003022244518487216 30-31 0.0015310930380001316 32-33 0.009119988965479045 34-35 0.0017973700880871111 36-37 8.121450027652871E-4 38-39 0.0011583051678783604 40-41 1.730800825565366E-4 42-43 0.005645073461843963 44-45 0.0016642315630436214 46-47 1.5976623005218766E-4 48-49 9.31969675304428E-4 50-51 9.319696753044279E-5 52-53 2.2633549257393247E-4 54-55 0.0018373116456001579 56-57 0.01653580481040142 58-59 0.02817211189920242 60-61 0.022673490814906296 62-63 0.015736973660140482 64-65 0.01989089564149736 66-67 0.003221952306052451 68-69 0.0015444068905044806 70-71 9.319696753044279E-5 72-73 1.331385250434897E-5 74-75 0.0019038809081219027 76-77 0.0023698657457741166 78-79 0.010198411018331311 80-81 0.018413058013514626 82-83 0.01922520301627991 84-85 0.03072837158003742 86-87 0.011583051678783604 88-89 0.04224485399629928 90-91 0.04802306598318674 92-93 0.02957006641215906 94-95 0.031753538222872295 96-97 0.05144472607680442 98-99 0.05413412428268292 100-101 0.060764422829848695 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3755487.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.42814080421067 #Duplication Level Percentage of deduplicated Percentage of total 1 69.97922728059336 33.88963872114436 2 15.642391604872827 15.150638863107682 3 5.897190244915981 8.56769878590026 4 2.912927430736689 5.64270639072656 5 1.5722290376583645 3.8070064606093967 6 0.9397473555027014 2.7306130351601667 7 0.6189942473108077 2.0983718396034936 8 0.42842181196937584 1.6598137466918401 9 0.3104236416955678 1.3529915846089922 >10 1.5283430565601352 13.134490677756187 >50 0.10702626056810155 3.5826881765366836 >100 0.0580055672010633 5.002292281995179 >500 0.0034183972539850237 1.1913749619144005 >1k 0.0014335214062418118 1.367201480108173 >5k 1.654063161048243E-4 0.54037621190589 >10k+ 5.513543870160811E-5 0.2820967822307406 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10565 0.2813217034168937 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8099 0.2156577828654446 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6954 0.18516906063048547 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5185 0.1380646504700988 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4380 0.11662934793809697 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4012 0.10683035249489613 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 5.325541001739588E-5 0.0 0.0 0.0 3 0.0 5.325541001739588E-5 0.0 2.662770500869794E-5 0.0 4 0.0 7.988311502609381E-5 0.0 2.662770500869794E-5 0.0 5 0.0 7.988311502609381E-5 0.0 2.662770500869794E-5 0.0 6 0.0 7.988311502609381E-5 0.0 2.662770500869794E-5 0.0 7 0.0 1.0651082003479176E-4 0.0 2.662770500869794E-5 0.0 8 0.0 1.0651082003479176E-4 0.0 2.662770500869794E-5 0.0 9 0.0 1.0651082003479176E-4 0.0 2.662770500869794E-5 0.0 10-11 0.0 1.0651082003479176E-4 0.0 2.662770500869794E-5 0.0 12-13 0.0 1.0651082003479176E-4 0.0 2.662770500869794E-5 0.0 14-15 0.0 1.1982467253914073E-4 0.0 2.662770500869794E-5 0.0 16-17 0.0 1.331385250434897E-4 0.0 2.662770500869794E-5 0.0 18-19 0.0 1.331385250434897E-4 0.0 3.994155751304691E-5 0.0 20-21 0.0 1.331385250434897E-4 0.0 6.656926252174484E-5 0.0 22-23 0.0 1.331385250434897E-4 0.0 1.331385250434897E-4 0.0 24-25 0.0 1.331385250434897E-4 0.0 1.4645237754783868E-4 0.0 26-27 0.0 1.331385250434897E-4 0.0 3.062186076000263E-4 0.0 28-29 0.0 1.4645237754783868E-4 0.0 6.257510677044016E-4 0.0 30-31 0.0 1.5976623005218763E-4 0.0 0.0014245822179653398 0.0 32-33 0.0 1.5976623005218763E-4 0.0 0.0026494566483654452 0.0 34-35 0.0 1.5976623005218763E-4 0.0 0.003967528046295993 0.0 36-37 0.0 1.5976623005218763E-4 0.0 0.005738270429374406 0.0 38-39 0.0 1.5976623005218763E-4 0.0 0.008174705437670267 0.0 40-41 0.0 1.5976623005218763E-4 0.0 0.013540187996922902 0.0 42-43 0.0 1.5976623005218763E-4 0.0 0.01809352555341025 0.0 44-45 0.0 1.5976623005218763E-4 0.0 0.024404291640471663 0.0 46-47 0.0 1.5976623005218763E-4 0.0 0.03605391258177701 0.0 48-49 0.0 1.5976623005218763E-4 0.0 0.04428187342946467 0.0 50-51 0.0 1.5976623005218763E-4 0.0 0.05525248789304822 0.0 52-53 0.0 1.5976623005218763E-4 1.331385250434897E-5 0.06908558064506681 0.0 54-55 2.662770500869794E-5 1.5976623005218763E-4 2.662770500869794E-5 0.08772497415115536 0.0 56-57 2.662770500869794E-5 1.5976623005218763E-4 2.662770500869794E-5 0.12773310092672402 0.0 58-59 2.662770500869794E-5 1.5976623005218763E-4 2.662770500869794E-5 0.16814064327742315 0.0 60-61 2.662770500869794E-5 1.5976623005218763E-4 2.662770500869794E-5 0.203502235528974 0.0 62-63 2.662770500869794E-5 1.5976623005218763E-4 2.662770500869794E-5 0.24408285796222967 1.331385250434897E-5 64-65 2.662770500869794E-5 1.5976623005218763E-4 2.662770500869794E-5 0.28749933097891167 2.662770500869794E-5 66-67 2.662770500869794E-5 1.5976623005218763E-4 2.662770500869794E-5 0.35497393547095224 2.662770500869794E-5 68-69 2.662770500869794E-5 1.5976623005218763E-4 2.662770500869794E-5 0.439397074201029 2.662770500869794E-5 70-71 2.662770500869794E-5 1.5976623005218763E-4 2.662770500869794E-5 0.48552957312859824 2.662770500869794E-5 72-73 2.662770500869794E-5 1.5976623005218763E-4 2.662770500869794E-5 0.5365349420727592 2.662770500869794E-5 74-75 2.662770500869794E-5 1.5976623005218763E-4 2.662770500869794E-5 0.6247392149140711 2.662770500869794E-5 76-77 2.662770500869794E-5 1.5976623005218763E-4 2.662770500869794E-5 0.7319689829840976 2.662770500869794E-5 78-79 2.662770500869794E-5 1.5976623005218763E-4 2.662770500869794E-5 0.8381735844112894 2.662770500869794E-5 80-81 2.662770500869794E-5 1.5976623005218763E-4 2.662770500869794E-5 0.9378677119638545 2.662770500869794E-5 82-83 2.662770500869794E-5 1.5976623005218763E-4 2.662770500869794E-5 1.071006237007344 2.662770500869794E-5 84-85 2.662770500869794E-5 1.5976623005218763E-4 2.662770500869794E-5 1.2628987931525257 2.662770500869794E-5 86-87 2.662770500869794E-5 1.5976623005218763E-4 2.662770500869794E-5 1.48235102398171 2.662770500869794E-5 88-89 2.662770500869794E-5 1.5976623005218763E-4 2.662770500869794E-5 1.7515704354721504 2.662770500869794E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11665 0.0 22.76912 1 TACATGG 11890 0.0 21.57525 2 GAGTACT 11170 0.0 21.365408 12-13 ACATGGG 11795 0.0 20.819904 3 GTACTTT 11870 0.0 19.804571 14-15 AGTACTT 11695 0.0 19.553493 12-13 AGAGTAC 17350 0.0 19.407486 10-11 GTATCAA 24530 0.0 17.85881 1 ACCGTCG 295 6.362461E-8 17.708729 8 CATGGGG 7185 0.0 17.118992 4 GGTATCA 20110 0.0 16.893248 1 TACTTTT 13930 0.0 16.48376 14-15 ACTTTTT 14550 0.0 16.482897 16-17 GAGTACA 9470 0.0 15.954983 1 ATACCGT 485 2.5465852E-11 15.666703 6 CCTATAC 880 0.0 15.653139 3 TCAACGC 27740 0.0 15.630379 4 CAACGCA 27780 0.0 15.624968 5 TAAGGTG 1520 0.0 15.621819 5 ATCAACG 27775 0.0 15.57939 3 >>END_MODULE